1. Antimicrobial peptides in saliva of children with severe early childhood caries
- Author
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Laís Fernanda Fonseca Ribas, Natália Helena Colombo, Paula Fernanda Kreling, Anne C.R. Tanner, Jesse Augusto Pereira, Cristiane Duque, Christine A. Kressirer, Universidade Estadual Paulista (Unesp), Forsyth Institute, and Harvard School of Dental Medicine
- Subjects
Male ,0301 basic medicine ,Saliva ,beta-Defensins ,Histatin-5 ,Human cathelicidin LL-37 ,medicine.medical_treatment ,Antimicrobial peptides ,Histatins ,Dental Caries ,Cathelicidin ,Salivary Peptides ,Streptococcus mutans ,Defensins ,03 medical and health sciences ,0302 clinical medicine ,Anti-Infective Agents ,Cathelicidins ,medicine ,Humans ,Salivary Proteins and Peptides ,Children ,General Dentistry ,Innate immunity ,biology ,business.industry ,030206 dentistry ,Cell Biology ,General Medicine ,biology.organism_classification ,medicine.disease ,stomatognathic diseases ,030104 developmental biology ,Beta defensin ,Otorhinolaryngology ,Child, Preschool ,Immunology ,Dental caries ,Female ,business ,Early childhood caries ,Antimicrobial Cationic Peptides - Abstract
Made available in DSpace on 2018-12-11T17:02:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-09-01 Objective Controversies exist regarding the relationship between the concentrations of antimicrobial peptides (AMPs) and presence of dental caries in children. Thus, the aim of this study was to examine levels of AMPs in saliva of caries-free (CF), early childhood caries (ECC) and severe early childhood caries (S-ECC) children to determine if the levels of these salivary peptides individually or in combinations were related to caries severity and mutans streptococci levels. Design 36 to 60 month-old children were selected to participate in this study. Children were grouped into CF group (n = 29), ECC group (n = 25) and S-ECC group (n = 29). Saliva was collected from children for microbiological analysis by culture. Salivary concentrations of cathelicidin LL-37, human β-defensin 2 (hBD-2), human β-defensin 3 (hBD-3) and histatin-5 (HTN-5) were determined by ELISA. Results Salivary concentrations of AMPs did not differ among CF, ECC and S-ECC groups. Data showed positive correlations between mutans streptococci levels and salivary hBD-2 or HTN-5. Positive correlations were found between hBD-2, hBD-3, LL-37 and HTN-5. Combinations among AMPs, mainly LL-37, were positively associated with caries levels. Conclusions Salivary concentrations of AMPs individually were not associated with the severity of early childhood caries. The stimulus of caries appears to trigger a biological response, however, with a combination of these peptides. UNESP-Univ. Estadual Paulista Department of Pediatric Dentistry and Public Health Araçatuba Dental School, Rua José Bonifácio, 1193 Forsyth Institute Department of Microbiology Harvard University Harvard School of Dental Medicine UNESP-Univ. Estadual Paulista Department of Pediatric Dentistry and Public Health Araçatuba Dental School, Rua José Bonifácio, 1193
- Published
- 2016