204 results on '"Jun-Hee Park"'
Search Results
2. Infrared thermography for automatic condition-based monitoring in high voltage electrical equipment (Conference Presentation)
- Author
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Jun Su Lee, Ju-Sik Kim, Jun-Hee Park, and Joo-Hyung Kim
- Published
- 2023
3. Electrochemical One-Step Immunoassay Based on Switching Peptides and Pyrolyzed Carbon Electrodes
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Jun-Hee Park, Zhiquan Song, Ji-Hong Bong, Hong-Rae Kim, Moon-Ju Kim, Kyung-Hak Choi, Seung-Shick Shin, Min-Jung Kang, Do Young Lee, and Jae-Chul Pyun
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Immunoassay ,Fluid Flow and Transfer Processes ,Immunoglobulin G ,Process Chemistry and Technology ,Animals ,Bioengineering ,Peptides ,Electrodes ,Instrumentation ,Carbon - Abstract
Switching peptides were designed to bind reversibly to the binding pocket of antibodies (IgG) by interacting with frame regions (FRs). These peptides can be quantitatively released when antigens bind to IgG. As FRs have conserved amino acid sequences, switching peptides can be used as antibodies for different antigens and different source animals. In this study, an electrochemical one-step immunoassay was conducted using switching peptides labeled with ferrocene for the quantitative measurement of analytes. For the effective amperometry of the switching peptides labeled with ferrocene, a pyrolyzed carbon electrode was prepared by pyrolysis of the parylene-C film. The feasibility of the pyrolyzed carbon electrode for the electrochemical one-step immunoassay was determined by analyzing its electrochemical properties, such as its low double-layer capacitance (
- Published
- 2022
4. Carbon electrode obtained via pyrolysis of plasma-deposited parylene-C for electrochemical immunoassays
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Zhiquan Song, Jun-Hee Park, Hong-Rae Kim, Ga-Yeon Lee, Min-Jung Kang, Moo-Hwan Kim, and Jae-Chul Pyun
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Electrochemistry ,Environmental Chemistry ,Biochemistry ,Spectroscopy ,Analytical Chemistry - Abstract
In this study, parylene-C films from plasma deposition as well as thermal deposition were pyrolyzed to prepare a carbon electrode for application in electrochemical immunoassays.
- Published
- 2022
5. Rapid Analysis of Bacterial Contamination in Platelets without Pre-Enrichment Using Pig Serum-Derived Antibodies
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Ga Yeon Lee, Jae Chul Pyun, Hyun Ok Kim, Jeong Soo Sung, Min Jung Kang, Ji Hong Bong, Jun Hee Park, and Chang Kyu Lee
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Blood Platelets ,Immunoassay ,biology ,Swine ,Chemistry ,Biochemistry (medical) ,Biomedical Engineering ,Membrane Proteins ,Biosensing Techniques ,General Chemistry ,Contamination ,Gram-Positive Bacteria ,Shelf life ,Antibodies ,Microbiology ,Biomaterials ,Pre enrichment ,Gram-Negative Bacteria ,Escherichia coli ,biology.protein ,Animals ,Platelet ,Antibody - Abstract
As the shelf life of platelets collected from donated blood is very short, approximately 5 days, the determination of bacterial contamination in platelets has become necessary. In this study, rapid analysis of Gram-positive and Gram-negative bacterial contamination in platelet samples was presented without pre-enrichment using pig serum-derived antibodies against the outer membrane proteins (OMP) of Gram-negative bacteria and antibodies against lipoteichoic acid (LTA) on the surface of Gram-positive bacteria. The anti-OMP antibodies against Gram-negative bacteria were isolated using sequential incubation with (1) the modified Gram-negative bacteria ClearColi, which lacks lipopolysaccharide (LPS) on the outer membrane, and (2) the Gram-positive bacteria
- Published
- 2021
6. One-step immunoassay for the detection of food-poisoning related bacteria using a switching peptide
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Chang Kyu Lee, Jaeyong Jung, Hong-Rae Kim, Ji-Hong Bong, Tae-Hun Kim, Jun-Hee Park, Soonil Kwon, Min-Jung Kang, and Jae-Chul Pyun
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Immunoassay ,Bacteria ,Electrochemistry ,Fluorescence Resonance Energy Transfer ,Environmental Chemistry ,Peptides ,Biochemistry ,Spectroscopy ,Antibodies ,Analytical Chemistry - Abstract
A one-step immunoassay was developed for five types of food-poisoning-related bacteria using a switching peptide and antibodies isolated from unimmunized horse serum. The one-step immunoassay involves mixing samples and reagents in a homogeneous solution without any washing steps. In this work, a one-step immunoassay configuration was developed using isolated antibodies labelled with an organic fluorescence quencher and a switching-peptide labelled with a fluorescent dye. The fluorescence-labelled switching-peptide was bound to the antigen-binding site of the isolated antibodies before binding to the bacteria (no fluorescence signal), and the switching-peptide dissociated from the antibodies as soon as they bound to the bacteria (fluorescence signal turns on). By quantifying the generated fluorescence signal, the one-step immunoassay presented here allows microbial detection without any washing step.
- Published
- 2022
7. Immunoaffinity biosensors for the detection of SARS-CoV-1 using screened Fv-antibodies from an autodisplayed Fv-antibody library
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Jaeyong Jung, Ji-Hong Bong, Jeong Soo Sung, Jun-Hee Park, Tae-Hun Kim, Soonil Kwon, Min-Jung Kang, Joachim Jose, and Jae-Chul Pyun
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Electrochemistry ,Biomedical Engineering ,Biophysics ,General Medicine ,Biotechnology - Published
- 2023
8. Cesium Lead Bromide (CsPbBr3) Perovskite Quantum Dot-Based Photosensor for Chemiluminescence Immunoassays
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Jae Chul Pyun, Jun Hee Park, Hong-Rae Kim, Dong Hee Son, Ji-Hong Bong, Min Jung Kang, and Zhiquan Song
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Photomultiplier ,Materials science ,Fabrication ,Passivation ,medicine.diagnostic_test ,business.industry ,Photodetector ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,law.invention ,Quantum dot ,law ,Immunoassay ,medicine ,Optoelectronics ,General Materials Science ,0210 nano-technology ,business ,Perovskite (structure) ,Chemiluminescence - Abstract
Chemiluminescence immunoassays have been widely employed for diagnosing various diseases. However, because of the extremely low intensity chemiluminescence signals, highly sensitive transducers, such as photomultiplier tubes and image sensors with cooling devices, are required to overcome this drawback. In this study, a hypersensitive photosensor was developed based on cesium lead bromide (CsPbBr3) perovskite quantum dots (QDs) with sufficient high sensitivity for chemiluminescence immunoassays. First, CsPbBr3 QDs with a highly uniform size, that is, 5 nm, were synthesized under thermodynamic control to achieve a high size confinement effect. For the fabrication of the photosensor, MoS2 nanoflakes were used as an electron transfer layer and heat-treated at an optimum temperature. Additionally, a parylene-C film was used as a passivation layer to improve the physical stability and sensitivity of the photosensor. In particular, the trap states on the CsPbBr3 QDs were reduced by the passivation layer, and the sensitivity was increased. Finally, a photosensor based on CsPbBr3 QDs was employed in chemiluminescence immunoassays for the detection of human hepatitis B surface antigen, human immunodeficiency virus antibody, and alpha-fetoprotein (AFP, a cancer biomarker). When compared with the conventionally used equipment, the photosensor was determined to be feasible for application in chemiluminescence immunoassays.
- Published
- 2021
9. Carbon electrode obtained
- Author
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Zhiquan, Song, Jun-Hee, Park, Hong-Rae, Kim, Ga-Yeon, Lee, Min-Jung, Kang, Moo-Hwan, Kim, and Jae-Chul, Pyun
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Immunoassay ,Polymers ,Humans ,Xylenes ,Electrodes ,Carbon ,Pyrolysis - Abstract
In this study, parylene-C films from plasma deposition as well as thermal deposition were pyrolyzed to prepare a carbon electrode for application in electrochemical immunoassays. Plasma deposition could prepare parylene-C in a faster deposition rate and more precise control over the thickness in comparison with the conventional thermal deposition. To analyze the influence of the deposition method, the crystal and electronic structures of the pyrolyzed parylene-C films obtained
- Published
- 2022
10. Differentiation-promoting and Protective Effects of the Fractions of Various Ginseng Species in C2C12 Cells
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Ji Won Lee, Wan Kyunn Whang, Jun Kee Hong, Jun Hee Park, Sung Su Kim, Ji Won Moon, and Eun Young Hong
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Ginseng ,Traditional medicine ,Chemistry ,Pharmaceutical Science ,Plant Science ,Agronomy and Crop Science ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,C2C12 - Published
- 2021
11. Anti-SARS-CoV-2 Nucleoprotein Antibodies Derived from Pig Serum with a Controlled Specificity
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Jae Chul Pyun, Jaeyong Jung, Hyun Ok Kim, Ji Hong Bong, Chang Kyu Lee, Hong Rae Kim, Min Jung Kang, and Jun Hee Park
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2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,viruses ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Biomedical Engineering ,Bioengineering ,Surface plasmon resonance biosensor ,02 engineering and technology ,01 natural sciences ,medicine ,Electrical and Electronic Engineering ,skin and connective tissue diseases ,biology ,Strain (chemistry) ,medicine.diagnostic_test ,Chemistry ,fungi ,010401 analytical chemistry ,virus diseases ,021001 nanoscience & nanotechnology ,Molecular biology ,respiratory tract diseases ,0104 chemical sciences ,Nucleoprotein ,Immunoassay ,biology.protein ,Antibody ,0210 nano-technology ,Biotechnology - Abstract
Antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleoprotein (NP) were purified from pig serum through two steps: (1) isolation of anti-NP IgG antibodies using magnetic beads with immobilized human SARS-CoV-2 NP and (2) filtration of anti-spike protein (SP) IgG antibodies using magnetic beads with immobilized human SARS-CoV SP. The enhanced specificity of the purified antibodies to the NP of SARS-CoV-2 was demonstrated using an immunoassay with anti-NP IgG antibodies after the isolation and filtration steps. The binding constants (Kd) of the purified anti-NP IgG antibodies to the NP of SARS-CoV-2 and the SP of SARS-CoV were estimated using a surface plasmon resonance biosensor (SPR). A competitive assay using the two-step purified anti-NP IgG antibodies from pig serum demonstrated (a) the detection of SARS-CoV-2 in viral fluid and (b) the discrimination of SARS-CoV-2 from SARS-CoV, MERS-CoV, and CoV strain 229E in viral fluids.
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- 2021
12. The downregulation of IGFBP3 by TGF-β signaling in oral cancer contributes to the osteoclast differentiation
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Jun-Hee Park, Won Yoon Chung, and Min Ju Jung
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Male ,0301 basic medicine ,Osteolysis ,medicine.medical_treatment ,Biophysics ,Down-Regulation ,Osteoclasts ,Biochemistry ,Small hairpin RNA ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Osteogenesis ,Transforming Growth Factor beta ,Osteoclast ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Insulin-Like Growth Factor I ,Molecular Biology ,Mice, Inbred ICR ,Squamous Cell Carcinoma of Head and Neck ,Chemistry ,Macrophages ,Growth factor ,RANK Ligand ,Osteoprotegerin ,Receptor, Transforming Growth Factor-beta Type II ,Cancer ,Cell Differentiation ,Cell Biology ,medicine.disease ,Insulin-Like Growth Factor Binding Protein 3 ,030104 developmental biology ,Cytokine ,medicine.anatomical_structure ,Matrix Metalloproteinase 9 ,Gene Knockdown Techniques ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,Mouth Neoplasms ,Bone marrow ,Signal Transduction - Abstract
Oral squamous cell carcinoma (OSCC) frequently invades nearby bone and bone involvement determines the prognosis of patients. Growth factors, stored in the bone matrix and released during bone destruction, are known as key components in the bone-tumor interaction. However, the coordination of growth factor signals and the precise mechanism of bone destruction in oral cancer are still unclear. In the study, we investigated the differential cytokine expression profile of oral cancer cells by TGF-β treatment and the function of altered expression of cytokines on the osteoclast differentiation. We established TGFBR2-knockdown cells using small hairpin RNA. TGF-β was treated to both TGFBR2 expressing and knockdown cells and the culture supernatants were analyzed using a cytokine array kit. We found that the TGF-β inhibited IGFBP3 level and enhanced MMP9 level. We confirmed this regulation of IGFBP3 and MMP9 by TGF-β using ELISA and zymography, respectively. IGFBP3 is known as to modulate the bioavailability of IGF1, which is abundant in the bone microenvironment and regulates osteoclast differentiation. Therefore, we further analyzed the function of IGFBP3 on osteoclastogenesis. Although IGFBP3 increased the viability of murine bone marrow macrophages, the osteoclast differentiation of these cells was blocked by IGFBP3 in a dose-dependent manner. These results revealed a novel pathway for the regulation of osteoclastogenesis by oral cancer cells, which may be a new therapeutic target for osteolysis induced by oral cancer infiltrating into the bone.
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- 2021
13. Differentiating and quantifying exosome secretion from a single cell using quasi-bound states in the continuum
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Liyi Hsu, Jun-Hee Park, Rushin Contractor, Boubacar Kante, Yu-Hwa Lo, Abdoulaye Ndao, Jeongho Ha, and Wei Cai
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Physics ,Continuum (topology) ,QC1-999 ,Cell ,exosomes ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,Exosome ,Atomic and Molecular Physics, and Optics ,Microvesicles ,Electronic, Optical and Magnetic Materials ,010309 optics ,medicine.anatomical_structure ,bound states in continuum ,0103 physical sciences ,Bound state ,medicine ,Biophysics ,Secretion ,Electrical and Electronic Engineering ,0210 nano-technology ,sensing ,Biotechnology - Abstract
One of the key challenges in biology is to understand how individual cells process information and respond to perturbations. However, most of the existing single-cell analysis methods can only provide a glimpse of cell properties at specific time points and are unable to provide cell secretion and protein analysis at single-cell resolution. To address the limits of existing methods and to accelerate discoveries from single-cell studies, we propose and experimentally demonstrate a new sensor based on bound states in the continuum to quantify exosome secretion from a single cell. Our optical sensors demonstrate high-sensitivity refractive index detection. Because of the strong overlap between the medium supporting the mode and the analytes, such an optical cavity has a figure of merit of 677 and sensitivity of 440 nm/RIU. Such results facilitate technological progress for highly conducive optical sensors for different biomedical applications.
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- 2020
14. Differentiation of adipose-derived stem cells into functional chondrocytes by a small molecule that induces Sox9
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Byeong-Wook Song, Jun-Hee Park, Sang Woo Kim, Sunhye Shin, Jiyun Lee, Chang Youn Lee, Jong Chul Park, Hyang-Hee Seo, Il-Kwon Kim, Soyeon Lim, Seahyoung Lee, and Ki-Chul Hwang
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Male ,0301 basic medicine ,medicine.medical_treatment ,Cellular differentiation ,Clinical Biochemistry ,Stem-cell differentiation ,QD415-436 ,Biology ,Biochemistry ,Article ,Chondrocyte ,Cell Line ,Cell therapy ,03 medical and health sciences ,Chondrocytes ,0302 clinical medicine ,Osteoarthritis ,medicine ,Animals ,Humans ,Bone regeneration ,Molecular Biology ,Cell Proliferation ,Stem Cells ,Cartilage ,Regeneration (biology) ,Cell Differentiation ,SOX9 Transcription Factor ,Stem-cell therapy ,Rats ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,Adipose Tissue ,Gene Expression Regulation ,030220 oncology & carcinogenesis ,Medicine ,Molecular Medicine ,Tumor Suppressor Protein p53 ,Stem cell ,Chondrogenesis ,Biomarkers - Abstract
Osteoarthritis (OA) is a common joint disease that results from the disintegration of joint cartilage and the underlying bone. Because cartilage and chondrocytes lack the ability to self-regenerate, efforts have been made to utilize stem cells to treat OA. Although various methods have been used to differentiate stem cells into functional chondrocytes, the currently available methods cannot induce stem cells to undergo differentiation into chondrocyte-like cells without inducing characteristics of hypertrophic chondrocytes, which finally lead to cartilage disintegration and calcification. Therefore, an optimized method to differentiate stem cells into chondrocytes that do not display undesired phenotypes is needed. This study focused on differentiating adipose-derived stem cells (ASCs) into functional chondrocytes using a small molecule that regulated the expression of Sox9 as a key factor in cartilage development and then explored its ability to treat OA. We selected ellipticine (ELPC), which induces chondrocyte differentiation of ASCs, using a GFP-Sox9 promoter vector screening system. An in vivo study was performed to confirm the recovery rate of cartilage regeneration with ASC differentiation into chondrocytes by ELPC in a collagenase-induced animal model of OA. Taken together, these data indicate that ellipticine induces ASCs to differentiate into mature chondrocytes without hypertrophic chondrocytes in vitro and in vivo, thus overcoming a problem encountered in previous studies. These results indicate that ELPC is a novel chondrocyte differentiation-inducing drug that shows potential as a cell therapy for OA., Osteoarthritis: Drug-induced stem cell differentiation shows therapeutic promise A novel method of generating healthy cartilage cells from stem cells could repair and regenerate joints and bones damaged by osteoarthritis. The cells that produce and maintain cartilage tissue are known as chondrocytes. Stem cell therapy could potentially re-start cartilage and bone regeneration in patients with osteoarthritis, however scientists have struggled to successfully induce stem cells to differentiate into functioning chondrocytes. A new method developed by Soyeon Lim and Ki-Chul Hwang at the Catholic Kwandong University, Gangneung, South Korea, and co-workers uses a drug called ellipticine to boost the expression of Sox-9, a key protein involved in cartilage development. The researchers found that the ellipticine-based method induced a mature, healthy chondrocyte population in cell cultures and rat models of osteoarthritis. Rats injected with these cells recovered from cartilage damage, suggesting a potential osteoarthritis therapy.
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- 2020
15. Clinical Significance of Jagged-1 Activated by APEX1 as a Chemoresistance Factor in Advanced Gastric Cancer
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Sang-Gon Park, Hee-Jeong Lee, Gee-Beum Kim, Hong-Beum Kim, Jun Hee Park, and Hyun-Jong Lim
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Cancer Research ,Apoptosis ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Stomach Neoplasms ,Cell Line, Tumor ,DNA-(Apurinic or Apyrimidinic Site) Lyase ,medicine ,Animals ,Humans ,MTT assay ,Clinical significance ,IC50 ,Cell Proliferation ,Neoplasm Staging ,Cisplatin ,Gene knockdown ,business.industry ,Stomach ,General Medicine ,Xenograft Model Antitumor Assays ,Staining ,Oncology ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,Immunohistochemistry ,Fluorouracil ,business ,Jagged-1 Protein ,medicine.drug - Abstract
Background/aim We investigated the clinical role of the molecular targets, APEX1 and Jagged-1, and the Apex1 - Jagged-1 cascade in gastric cancer cells. Materials and methods We used 6 human gastric cancer cell lines (SNU-1, SNU-5, SNU-16, NCI-N87, KATO- III and AGS), and demonstrated the chemosensitivity of APEX1 and Jagged-1 through the MTT assay and immunoblotting. Tumor growth was assayed following cisplatin and 5-FU treatment using a xenograft model injected with KATO-III cells. Moreover, gastric tumor samples from 9 patients, divided in 2 groups according to chemotherapy response, were examined by immunocytochemical (IHC) staining, and protein expression levels were scored. Results Following APEX1 knockdown, the MTT assay revealed that the IC50 of cisplatin and 5-FU in AGS cells was decreased approximately 7% and 15%, respectively, however, their decrease in chemoresistant KATO-III cells was decreased by approximately 21% and 67% for cisplatin and 5-FU, respectively. The tumor volume of KATO-III/sicontrol mice treated with cisplatin and 5-FU was affected less, compared with KATO-III/siAPEX1 mice treated with cisplatin and 5-FU. Also, the expression levels of APEX1, Jagged-1 and CD133, assayed by IHC staining, were higher in the chemorefractory group than in the chemoresponsive group. Conclusion Jagged-1-activated signaling by APEX1 plays a role in advanced gastric cancer.
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- 2020
16. Symmetry-breaking-induced plasmonic exceptional points and nanoscale sensing
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Jun-Hee Park, Ashok Kodigala, Boubacar Kante, Abdoulaye Ndao, Liyi Hsu, Thomas Lepetit, Wei Cai, and Yu-Hwa Lo
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Diffraction ,Physics ,Photon ,business.industry ,Avoided crossing ,Optical physics ,Physics::Optics ,General Physics and Astronomy ,Metamaterial ,01 natural sciences ,010305 fluids & plasmas ,Wavelength ,0103 physical sciences ,Optoelectronics ,Symmetry breaking ,010306 general physics ,business ,Plasmon - Abstract
Singularities of open systems, known as exceptional points (EPs), have been shown to exhibit increased sensitivities, but the observation of EPs has so far been limited to wavelength-scaled systems subject to the diffraction limit. Plasmons, the collective oscillations of free electrons coupled to photons, shrink the wavelength of light to electronic and molecular length scales. We propose a novel approach to EPs based on spatial symmetry breaking and report their observation in plasmonics at room temperature. The plasmonic EPs are based on the hybridization of detuned resonances in multilayered plasmonic structures to reach a critical complex coupling rate between nanoantenna arrays, resulting in the simultaneous coalescence of the resonances and loss rates. Their utility as sensors of anti-immunoglobulin G, the most abundant immunoglobulin isotype in human serum, is evaluated. Our work opens the way to a new class of nanoscale devices, sensors and imagers based on topological polaritonic effects. The hybridized modes of an asymmetric plasmonic dimer show avoided crossing of both the real and imaginary parts. This can lead to plasmonic exceptional points, which are used for biosensing with very high sensitivity.
- Published
- 2020
17. Effect of the Artistic Value and Brand Asset of Taekwondo Performances Perceived by University Taekwondo Demonstration Player’s
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Jun-Hee Park
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Microeconomics ,Value (economics) ,Economics ,General Medicine ,Asset (economics) - Published
- 2020
18. Enhanced anti-tumor immunity of vaccine combined with anti-PD-1 antibody in a murine bladder cancer model
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Soyeon Lim, Jun-Hee Park, and Hyun Chang
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Urology - Published
- 2023
19. CCL28-induced RARβ expression inhibits oral squamous cell carcinoma bone invasion
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Jun-Hee Park, Na-Young Song, Sun Kyoung Lee, Won Yoon Chung, Kwang Kyun Park, Ki Rim Kim, Xianglan Zhang, Jae Hoon Shim, and Seung Hwa Son
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Male ,0301 basic medicine ,Chemokine ,Epithelial-Mesenchymal Transition ,Osteolysis ,Bone disease ,Receptors, Retinoic Acid ,Osteoclasts ,Histone Deacetylase 1 ,Receptors, CCR10 ,Bone and Bones ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Osteoclast ,Cell Line, Tumor ,Animals ,Humans ,Medicine ,Neoplasm Invasiveness ,Mice, Inbred BALB C ,Mice, Inbred ICR ,biology ,Squamous Cell Carcinoma of Head and Neck ,business.industry ,Retinoic Acid Receptor alpha ,RANK Ligand ,Cancer ,General Medicine ,medicine.disease ,stomatognathic diseases ,Retinoic acid receptor ,030104 developmental biology ,medicine.anatomical_structure ,Chemokines, CC ,030220 oncology & carcinogenesis ,biology.protein ,Cancer research ,CCL28 ,Mouth Neoplasms ,business ,Research Article - Abstract
Oral squamous cell carcinoma (OSCC) frequently invades the maxillary or mandibular bone, and this bone invasion is closely associated with poor prognosis and survival. Here, we show that CCL28 functions as a negative regulator of OSCC bone invasion. CCL28 inhibited invasion and epithelial-mesenchymal transition (EMT), and its inhibition of EMT was characterized by induced E-cadherin expression and reduced nuclear localization of β-catenin in OSCC cells with detectable RUNX3 expression levels. CCL28 signaling via CCR10 increased retinoic acid receptor-β (RARβ) expression by reducing the interaction between RARα and HDAC1. In addition, CCL28 reduced RANKL production in OSCC and osteoblastic cells and blocked RANKL-induced osteoclastogenesis in osteoclast precursors. Intraperitoneally administered CCL28 inhibited tumor growth and osteolysis in mouse calvaria and tibia inoculated with OSCC cells. RARβ expression was also increased in tumor tissues. In patients with OSCC, low CCL28, CCR10, and RARβ expression levels were highly correlated with bone invasion. Patients with OSCC who had higher expression of CCL28, CCR10, or RARβ had significantly better overall survival. These findings suggest that CCL28, CCR10, and RARβ are useful markers for the prediction and treatment of OSCC bone invasion. Furthermore, CCL28 upregulation in OSCC cells or CCL28 treatment can be a therapeutic strategy for OSCC bone invasion.
- Published
- 2019
20. Material enhancements of newly developed stiff type polyurea for retrofitting of concrete structures
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Tae-Hee Lee, Jun-Hee Park, Dal-Hun Yang, Jang-Ho Jay Kim, and Norhazilan Bin Md. Noor
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Materials Science (miscellaneous) - Published
- 2022
21. Pixel-based Continuous State Prediction with Perceptual Loss
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Donghun Lee and Jun Hee Park
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- 2021
22. Cesium Lead Bromide (CsPbBr
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Hong-Rae, Kim, Ji-Hong, Bong, Jun-Hee, Park, Zhiquan, Song, Min-Jung, Kang, Dong Hee, Son, and Jae-Chul, Pyun
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Immunoassay ,Titanium ,Hepatitis B Surface Antigens ,Lead ,Polymers ,Luminescent Measurements ,Quantum Dots ,Cesium ,Humans ,Oxides ,Calcium Compounds ,HIV Antibodies ,Xylenes - Abstract
Chemiluminescence immunoassays have been widely employed for diagnosing various diseases. However, because of the extremely low intensity chemiluminescence signals, highly sensitive transducers, such as photomultiplier tubes and image sensors with cooling devices, are required to overcome this drawback. In this study, a hypersensitive photosensor was developed based on cesium lead bromide (CsPbBr
- Published
- 2021
23. Suppressing Pyroptosis Augments Post-Transplant Survival of Stem Cells and Cardiac Function Following Ischemic Injury
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Jung-Won Choi, Byeong-Wook Song, Seahyoung Lee, Jiyun Lee, Sang Woo Kim, Il-Kwon Kim, Seongtae Jeong, Gyoonhee Han, Chang Youn Lee, Hyang-Hee Seo, Sunhye Shin, Jun-Hee Park, Ki-Chul Hwang, and Soyeon Lim
- Subjects
0301 basic medicine ,Male ,Necrosis ,Cardiac fibrosis ,Cell ,030204 cardiovascular system & hematology ,Rats, Sprague-Dawley ,Mice ,0302 clinical medicine ,pyroptosis ,M1 macrophage ,stem cells ,miR-762 ,I/R injury ,Biology (General) ,Spectroscopy ,Stem Cells ,Pyroptosis ,General Medicine ,Computer Science Applications ,Chemistry ,medicine.anatomical_structure ,Stem cell ,medicine.symptom ,Programmed cell death ,QH301-705.5 ,Myocardial Reperfusion Injury ,Catalysis ,Article ,Inorganic Chemistry ,03 medical and health sciences ,medicine ,Animals ,Humans ,Physical and Theoretical Chemistry ,QD1-999 ,Molecular Biology ,business.industry ,Macrophages ,Organic Chemistry ,medicine.disease ,Rats ,Transplantation ,MicroRNAs ,030104 developmental biology ,RAW 264.7 Cells ,Apoptosis ,Cancer research ,business ,Stem Cell Transplantation - Abstract
The acute demise of stem cells following transplantation significantly compromises the efficacy of stem cell-based cell therapeutics for infarcted hearts. As the stem cells transplanted into the damaged heart are readily exposed to the hostile environment, it can be assumed that the acute death of the transplanted stem cells is also inflicted by the same environmental cues that caused massive death of the host cardiac cells. Pyroptosis, a highly inflammatory form of programmed cell death, has been added to the list of important cell death mechanisms in the damaged heart. However, unlike the well-established cell death mechanisms such as necrosis or apoptosis, the exact role and significance of pyroptosis in the acute death of transplanted stem cells have not been explored in depth. In the present study, we found that M1 macrophages mediate the pyroptosis in the ischemia/reperfusion (I/R) injured hearts and identified miRNA-762 as an important regulator of interleukin 1β production and subsequent pyroptosis. Delivery of exogenous miRNA-762 prior to transplantation significantly increased the post-transplant survival of stem cells and also significantly ameliorated cardiac fibrosis and heart functions following I/R injury. Our data strongly suggest that suppressing pyroptosis can be an effective adjuvant strategy to enhance the efficacy of stem cell-based therapeutics for diseased hearts.
- Published
- 2021
24. Effect of Online and Offline Voice Therapy Programs in Patients with Hyperfunctional Voice Disorder: A Case Study
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Ha-Na Lee, Jae-Yeon Yoo, and Jun-Hee Park
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Online and offline ,Speech and Hearing ,Linguistics and Language ,medicine.medical_specialty ,Voice therapy ,business.industry ,Communication ,medicine ,In patient ,Audiology ,business ,Voice Disorder - Published
- 2019
25. Effects of vocal aerobic treatment on voice improvement in patients with voice disorders*
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Jae-Yeon Yoo, Ha-Na Lee, and Jun-Hee Park
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medicine.medical_specialty ,business.industry ,medicine ,In patient ,Audiology ,business - Published
- 2019
26. Hypersensitive electrochemical immunoassays based on highly N-doped silicon carbide (SiC) electrode
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Ga Yeon Lee, Seong Min Jeong, Jun Hee Park, Zhiquan Song, Jae Chul Pyun, and Min Jung Kang
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Carbon Compounds, Inorganic ,Analytical chemistry ,Biosensing Techniques ,02 engineering and technology ,Electrochemistry ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,law.invention ,Luminol ,chemistry.chemical_compound ,stomatognathic system ,law ,Silicon carbide ,medicine ,Humans ,Environmental Chemistry ,Electrodes ,Spectroscopy ,Chemiluminescence ,Immunoassay ,Hepatitis B Surface Antigens ,medicine.diagnostic_test ,Benzidines ,Silicon Compounds ,010401 analytical chemistry ,HIV ,Electrochemical Techniques ,Chronoamperometry ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,chemistry ,Electrode ,0210 nano-technology ,Electrochemical window - Abstract
Highly N-doped SiC was presented as an optimal electrode for electrochemical immunoassays with a far higher sensitivity than chemiluminescence detection. As the first step, the electrochemical properties of highly N-doped SiC, such as the double-layer capacitance (Cdl), rate constant for electron transfer (kapp) and ideal polarizable potential range (electrochemical window) were analyzed and compared with those of Au, Pt, and graphite electrodes. The highly N-doped SiC electrode was used for the quantification of oxidized 3,3′,5,5′-tetramethylbenzidine (TMB) which was widely used as chromogenic substrate for commercialized immunoassay kits. In order to enhance the sensitivity for the quantification of the oxidized TMB the chronoamperometry was applied to avoid the background current of i-V measurement. Finally, the chronoamperometry based on the highly N-doped SiC electrode was applied to commercial immunoassay kits for the medical diagnosis of the human immunodeficiency virus (HIV) and the human hepatitis B surface antigen (hHBsAg). The chronoamperometric measurement based on the highly N-doped SiC electrode was proved to detect at far lower limits in comparison with the conventional optical density measurement as well as the chemiluminescence assay based on luminol as a chemiluminescent probe.
- Published
- 2019
27. Development of smartphone-based voice therapy program
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Jae-Yeon Yoo, Jun-Hee Park, and Ha-Na Lee
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Multimedia ,Voice therapy ,Psychology ,computer.software_genre ,computer - Published
- 2019
28. Capacitive biosensor based on vertically paired electrode with controlled parasitic capacitance
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Jae Chul Pyun, Min Jung Kang, Jun Hee Park, Sungbo Cho, Young Wook Chang, and Ga Yeon Lee
- Subjects
Materials science ,Capacitive measurement ,02 engineering and technology ,01 natural sciences ,chemistry.chemical_compound ,Parylene ,Parasitic capacitance ,Materials Chemistry ,Electrical and Electronic Engineering ,Instrumentation ,Electrical impedance ,business.industry ,010401 analytical chemistry ,Metals and Alloys ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,chemistry ,Electrode ,Optoelectronics ,Capacitive biosensor ,Nanometre ,0210 nano-technology ,business ,Sensitivity (electronics) - Abstract
A capacitive biosensor based on vertically paired electrodes with controlled parasitic capacitance is presented to improve the sensitivity of capacitive measurement. The vertically paired electrodes were fabricated with a parylene film as a dielectric layer, with the distance between the electrodes less than hundreds of nanometer. The problem of parasitic capacitance owing to the electrode configuration was analyzed according to the superposed area of the electrode. In this work, two kinds of vertically paired electrodes were fabricated to control the parasitic capacitance—square-type and circular-type electrodes with different superimposed areas of 21.8 (100%) and 9.3 (42%) mm2 and the same electrode area of 9.4 × 10−5 mm2, respectively. The effect of superimposed area of the vertically paired electrodes on capacitive measurement was analyzed using the electrodes. The sensitivity of capacitive measurement was observed to increase with a decrease in superimposed electrode area and frequency. Further, the effect of parasitic capacitance was estimated by computer simulation of the sensitivity of impedance and capacitive measurement when 10% change in RS or CS occurred. The results showed that adsorption of proteins could be sensitively measured when the parasitic capacitance decreased. Finally, the effect of superimposed area of the vertically paired electrodes was measured from the interaction between antigens (human serum albumin, HSA) and immobilized antibodies (anti-HSA antibodies).
- Published
- 2018
29. Exceptional points at the subwavelength scale
- Author
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Liyi Hsu, Boubacar Kante, Abdoulaye Ndao, Jun-Hee Park, and Mutasem Odeh
- Subjects
Singularity ,Scale (ratio) ,Exceptional point ,Computer science ,Path (graph theory) ,Physics::Optics ,Metamaterial ,ComputerApplications_COMPUTERSINOTHERSYSTEMS ,Plasmonic nanostructures ,Engineering physics ,Plasmon - Abstract
We present our latest work and investigation of exceptional point in plasmonic nanostructures. We show that the non-Hermitian singularity can be effectively deployed in small scale sensors and pave the path for engineering metamaterials with novel properties.
- Published
- 2021
30. Recent advances in stem cell therapy for neurodegenerative disease: Three dimensional tracing and its emerging use
- Author
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Ki-Chul Hwang, Jun-Hee Park, Byeong-Wook Song, Il-Kwon Kim, and Bomi Kim
- Subjects
Cell tracing ,Histology ,Tissue clearing ,business.industry ,medicine.medical_treatment ,Cell Biology ,Disease ,Stem-cell therapy ,Review ,Stem cells ,Tracing ,Neurodegenerative disease ,Three dimensional imaging ,Genetics ,Three-dimensional imaging ,Medicine ,Stem cell ,business ,Molecular Biology ,Neuroscience ,Genetics (clinical) - Abstract
Neurodegenerative disease is a brain disorder caused by the loss of structure and function of neurons that lowers the quality of human life. Apart from the limited potential for endogenous regeneration, stem cell-based therapies hold considerable promise for maintaining homeostatic tissue regeneration and enhancing plasticity. Despite many studies, there remains insufficient evidence for stem cell tracing and its correlation with endogenous neural cells in brain tissue with three-dimensional structures. Recent advancements in tissue optical clearing techniques have been developed to overcome the existing shortcomings of cross-sectional tissue analysis in thick and complex tissues. This review focuses on recent progress of stem cell treatments to improve neurodegenerative disease, and introduces tissue optical clearing techniques that can implement a three-dimensional image as a proof of concept. This review provides a more comprehensive understanding of stem cell tracing that will play an important role in evaluating therapeutic efficacy and cellular interrelationship for regeneration in neurodegenerative diseases.
- Published
- 2021
31. Capacitive biosensor based on vertically paired electrodes for the detection of SARS-CoV-2
- Author
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Jun-Hee Park, Ga-Yeon Lee, Zhiquan Song, Ji-Hong Bong, Young Wook Chang, Sungbo Cho, Min-Jung Kang, and Jae-Chul Pyun
- Subjects
Immunoassay ,Capacitive biosensor ,SARS-CoV-2 ,viruses ,Biomedical Engineering ,Biophysics ,COVID-19 ,Biosensing Techniques ,General Medicine ,Nucleoprotein (NP) ,Sensitivity and Specificity ,Article ,Electrochemistry ,Humans ,PEDOT:PSS ,Computer Simulation ,Electrodes ,Vertically paired electrode ,Biotechnology - Abstract
Vertically paired electrodes (VPEs) with multiple electrode pairs were developed for the enhancement of capacitive measurements by optimizing the electrode gap and number of electrode pairs. The electrode was fabricated using a conductive polymer layer of PEDOT:PSS instead of Ag and Pt metal electrodes to increase the VPE fabrication yield because the PEDOT:PSS layer could be effectively etched using a reactive dry etching process. In this study, sensitivity enhancement was realized by decreasing the electrode gap and increasing the number of VPE electrode pairs. Such an increase in sensitivity according to the electrode gap and the number of electrode pairs was estimated using a model analyte for an immunoassay. Additionally, a computer simulation was performed using VPEs with different electrode gaps and numbers of VPE electrode pairs. Finally, VPEs with multiple electrode pairs were applied for SARS-CoV-2 nucleoprotein (NP) detection. The capacitive biosensor based on the VPE with immobilized anti-SARS-CoV-2 NP was applied for the specific detection of SARS-CoV-2 in viral cultures. Using viral cultures of SARS-CoV-2, SARS-CoV, MERS-CoV, and CoV-strain 229E, the limit of detection (LOD) was estimated to satisfy the cutoff value (dilution factor of 1/800) for the medical diagnosis of COVID-19, and the assay results from the capacitive biosensor were compared with commercial rapid kit based on a lateral flow immunoassay.
- Published
- 2022
32. Bioassay-Guided Isolation of Anti-Alzheimer Active Components from the Aerial Parts of
- Author
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Jun Hee, Park and Wan Kyunn, Whang
- Subjects
Glycation End Products, Advanced ,Spectrometry, Mass, Electrospray Ionization ,Flavonols ,iridoid glycoside ,Article ,Inhibitory Concentration 50 ,Alzheimer Disease ,Hedyotis ,Aspartic Acid Endopeptidases ,Humans ,simultaneous analysis ,Glycosides ,Hedyotis diffusa ,Least-Squares Analysis ,Chromatography, High Pressure Liquid ,Plant Extracts ,Stereoisomerism ,Plant Components, Aerial ,Molecular Docking Simulation ,Calibration ,Iridoid Glycosides ,Acetylcholinesterase ,Linear Models ,Solvents ,flavonol glycoside ,Biological Assay ,Quercetin ,Amyloid Precursor Protein Secretases ,Alzheimer’s disease ,Protein Binding ,bioassay-guided isolation - Abstract
Previous studies have reported that Hedyotis diffusa Willdenow extract shows various biological activities on cerebropathia, such as neuroprotection and short-term memory enhancement. However, there has been a lack of studies on the inhibitory activity on neurodegenerative diseases such as Alzheimer’s disease (AD) through enzyme assays of H. diffusa. Therefore, H. diffusa extract and fractions were evaluated for their inhibitory effects through assays of enzymes related to AD, including acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), and β-site amyloid precursor protein cleaving enzyme 1 (BACE1), and on the formation of advanced glycation end-product (AGE). In this study, ten bioactive compounds, including nine iridoid glycosides 1–9 and one flavonol glycoside 10, were isolated from the ethyl acetate and n-butanol fractions of H. diffusa using a bioassay-guided approach. Compound 10 was the strongest inhibitor of cholinesterase, BACE1, and the formation of AGEs of all isolated compounds, while compound 5 had the lowest inhibitory activity. Compounds 3, 6, and 9 exhibited better inhibitory activity than other compounds on AChE, and two pairs of diastereomeric iridoid glycoside structures (compounds 4, 8, and 6, 7) showed higher inhibitory activity than others on BChE. In the BACE1 inhibitory assay, compounds 1–3 were good inhibitors, and compound 10 showed higher inhibitory activity than quercetin, the positive control. Moreover, compounds 1 and 3 were stronger inhibitors of the formation of AGE than aminoguanidine (AMG), the positive control. In conclusion, this study is significant since it demonstrated that the potential inhibitory activity of H. diffusa on enzymes related to AD and showed the potential use for further study as a natural medicine for AD treatment on the basis of the bioactive components isolated from H. diffusa.
- Published
- 2020
33. Robotic Behavioral Cloning Through Task Building
- Author
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Hyunseok Kim, Chan-Won Park, Young-Sung Son, Jinchul Choi, and Jun-Hee Park
- Subjects
Cloning (programming) ,Computer science ,05 social sciences ,Robot manipulator ,010501 environmental sciences ,01 natural sciences ,Robot learning ,Task (project management) ,Action (philosophy) ,Human–computer interaction ,0502 economics and business ,Trajectory ,Robot ,050207 economics ,0105 earth and related environmental sciences - Abstract
Robot learning by demonstration is a research paradigm that can play an important role in expanding areas where robots can be applied. An easy method to get a policy to reproduce the demonstrated behavior is to learn a model that maps directly from demonstrations to action. This is referred to as behavioral cloning (BC). In this paper, a novel robotic BC method for directly imitating a policy from trajectories generated by performing a task is proposed. The proposed method not only allows a user to obtain a demonstrated trajectory for performing a task by manipulating the robot, but also automatically generates a trajectory by having the robot perform the task on behalf of the user. Experiment results showed that the proposed method can effectively learn to make accurate behavior prediction for robot manipulators.
- Published
- 2020
34. Learning Robot Manipulation based on Modular Reward Shaping
- Author
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Hyunseok Kim, Chan-Won Park, Ingook Jang, Jun-Hee Park, and Seonghyun Kim
- Subjects
Computer science ,business.industry ,Deep learning ,020206 networking & telecommunications ,02 engineering and technology ,Modular design ,Robot control ,Action (philosophy) ,Human–computer interaction ,0202 electrical engineering, electronic engineering, information engineering ,Task analysis ,Robot ,Reinforcement learning ,020201 artificial intelligence & image processing ,Artificial intelligence ,business ,Video game - Abstract
Recently, reinforcement learning utilizing deep learning, called deep reinforcement learning, is being developed for discontinuous action space. It is shown that deep reinforcement learning has higher playing skills than those of human in the video game as Atari. The development of deep reinforcement learning for discontinuous action space, has been extended to the continuous action space as robot control area. The area of control deals with issues closely related to real world environment. However, deep reinforcement learning has limitations to solve control problems in the real world due to numerous trial-and-error based training. To address the limitations of learning in the real world, virtual world environments are used to emulate control problem in real world. In this paper, to solve a complex robot manipulation task, the learning robot manipulation based modular reward shaping is proposed. Since it is difficult to solve the complex task using only single primitive manipulation skill with a reward, it is required to combine various primitive manipulation skills with various rewards. In simulation results, it is shown that the combination of the reward functions affects to the robot manipulation performance.
- Published
- 2020
35. Learning Control Policy with Previous Experiences from Robot Simulator
- Author
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Hyunseok Kim, Seonghyun Kim, Chan-Won Park, Jun-Hee Park, and Donghun Lee
- Subjects
Computer science ,Control (management) ,Robot manipulator ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,Robot learning ,Field (computer science) ,0202 electrical engineering, electronic engineering, information engineering ,Key (cryptography) ,Reinforcement learning ,Robot ,020201 artificial intelligence & image processing ,Simulation ,0105 earth and related environmental sciences - Abstract
Advances in deep reinforcement learning enabled cost-efficient training of control policy of physical robot actions from robot simulators. Learning control policy in a simulated environment is cost-efficient over learning in a real environment. Reward engineering is one of the key components to train efficient control policy. For tasks with long horizons such as navigation and manipulation, a sparse reward is providing limited information. The robot simulator for a physical engine of physical robot manipulation has made it easy for researchers in the field of deep reinforcement learning to simulate complicated robot manipulation environments. In this paper, A robot manipulation simulator and a deep RL framework are utilized for implement a training control policy by utilizing previous experiences. For implementation, Recent innovation Hindsight Experience Replay (HER) algorithms with previous experiences to calculate dense rewards from a sparse reward is leveraged . Proposed implementation showed an approach to investigate the reward engineering method to formulate dense reward in robot manipulator tasks.
- Published
- 2020
36. An Experimental Study on Reinforcement Learning on IoT Devices with Distilled Knowledge
- Author
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Hyunseok Kim, Ingook Jang, Jun-Hee Park, Seonghyun Kim, and Chan-Won Park
- Subjects
Multimedia ,business.industry ,Computer science ,020208 electrical & electronic engineering ,0202 electrical engineering, electronic engineering, information engineering ,Reinforcement learning ,02 engineering and technology ,Internet of Things ,business ,computer.software_genre ,computer - Abstract
This paper provides an experimental study of reinforcement learning on IoT devices using distilled knowledge, whose a teacher with a well-trained model transfers to a student with a new model to be trained. The experimental results show that the distilled knowledge is effective to a new model training on IoT devices.
- Published
- 2020
37. Autonomous Mobile Robot Navigation in Indoor Environments: Mapping, Localization, and Planning
- Author
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Jun-Hee Park, Samyeul Noh, and Jiyoung Park
- Subjects
0209 industrial biotechnology ,Computer science ,Autonomous Navigation System ,Real-time computing ,Monte Carlo localization ,020206 networking & telecommunications ,Mobile robot ,02 engineering and technology ,Simultaneous localization and mapping ,Collision ,Mobile robot navigation ,Computer Science::Robotics ,020901 industrial engineering & automation ,0202 electrical engineering, electronic engineering, information engineering ,Collision avoidance - Abstract
Developing an autonomous indoor mobile robot navigation system from scratch is very difficult and it takes a long time. It requires a series of complex tasks such as handling given sensor inputs, calculating all the information needed for autonomous navigation, and controlling actuators required for movement. In this paper, an autonomous navigation system for indoor mobile robots is introduced mainly based on open source provided by the robot operating system. The presented system is capable of autonomously navigating an unstructured indoor environment avoiding collision with static or dynamic objects. To this end, the system consists of three main modules: mapping, localization, and planning. The mapping module builds a global map for an unknown environment by means of a simultaneous localization and mapping algorithm based on laser scanner data. The localization module estimates the mobile robot’s pose within the prebuilt map by way of an adaptive Monte Carlo localization approach. The planning module builds a local cost map for collision avoidance, generates collision-free trajectories to reach a goal pose based on the cost map, and produces control commands to follow the trajectories. The presented system has been tested not only in simulation environments built in the Gazebo simulator but also in real environments utilizing the Jackal mobile robot, to validate its performance for autonomous navigation including collision avoidance.
- Published
- 2020
38. Position-Based Visual Servoing of Multiple Robotic Manipulators: Verification in Gazebo Simulator
- Author
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Samyeul Noh, Chan-Won Park, and Jun-Hee Park
- Subjects
Inverse kinematics ,Computer science ,020208 electrical & electronic engineering ,GRASP ,Robot manipulator ,020206 networking & telecommunications ,02 engineering and technology ,Object (computer science) ,Visual servoing ,Task (project management) ,Position (vector) ,0202 electrical engineering, electronic engineering, information engineering ,Motion planning ,Robotic arm ,Simulation - Abstract
Today, a paradigm in the manufacturing industry has been shifted from mass production to small and custom production. To adapt to today’s industrial expectations, this paper presents position-based visual servoing of multiple robotic manipulators. The presented visual servoing consists of two modules: task planning and motion planning. The task planning module manipulates each robotic arm not to collide with each other by distributing a task to them in a systematic manner. The motion planning module employs the MoveIt framework to solve inverse kinematics for each robotic arm so that it moves each end-effector’s pose to the desired pose to complete the task. In addition, the module controls a robotic hand to grasp or release a target object. The presented visual servoing has been tested and verified in the Gazebo simulator.
- Published
- 2020
39. An Application of LSTM Recurrent Networks to Expanding of Search Tree Nodes in Symbolic Planning
- Author
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Joonmyun Cho, Jun-Hee Park, Chan-Won Park, and Young-Sung Son
- Subjects
Computer science ,business.industry ,Heuristic ,Node (networking) ,Deep learning ,0102 computer and information sciences ,02 engineering and technology ,01 natural sciences ,Search tree ,010201 computation theory & mathematics ,Simple (abstract algebra) ,0202 electrical engineering, electronic engineering, information engineering ,020201 artificial intelligence & image processing ,Artificial intelligence ,business - Abstract
Applying deep learning to symbolic planning is not straightforward because they differently internalize knowledge. One represents knowledge numerically while the other does symbolically. Hybrid methods that take strengths from both techniques will deliver better performance on automated planning. This paper presents a method to improve the performance of symbolic planning by applying LSTM recurrent networks to inferring the guidance knowledge for search tree node expansion. The networks learn sequential patterns across previous node expansions and generalizes the learned knowledge to predict promising actions and their probability scores at the present expansion step. The prediction consists with node cost estimation for heuristic search. A simple experiment vindicates the method of this research.
- Published
- 2020
40. The Present and Future of Continual Learning
- Author
-
Soonyong Song, Jun-Hee Park, and Heechul Bae
- Subjects
Categorization ,business.industry ,Computer science ,Lifelong learning ,Benchmark (computing) ,Robot ,Artificial intelligence ,Baseline (configuration management) ,Machine learning ,computer.software_genre ,business ,Continual learning ,computer - Abstract
This paper addresses a continual lifelong learning problem that learns incremental multiple tasks in real-world environments. We overview and summarize representative approaches and categorization of the state-of-the-art in continual learning. Comparable scenarios, benchmark datasets, and baseline approaches for different continual scenarios introduced in this paper. We suggested a comparison of the differences and similarities with other machine learning methods. We also report real-world applications, especially robots and healthcare fields. We summarize current states and suggest future direction of continual learning problems.
- Published
- 2020
41. Switching-peptides for one-step immunoassay and its application to the diagnosis of human hepatitis B
- Author
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Jae Chul Pyun, Hong Rae Kim, Hyun Ok Kim, Ji Hong Bong, Min Jung Kang, Jaeyong Jung, Kyung Hak Choi, Jeong Soo Sung, Do Young Lee, Jun Hee Park, Chang Kyu Lee, and Seong Shick Shin
- Subjects
HBsAg ,Biomedical Engineering ,Biophysics ,One-Step ,02 engineering and technology ,Biosensing Techniques ,01 natural sciences ,Antigen ,Electrochemistry ,medicine ,Animals ,Humans ,Detection limit ,Immunoassay ,Chromatography ,Hepatitis B Surface Antigens ,biology ,medicine.diagnostic_test ,Chemistry ,010401 analytical chemistry ,General Medicine ,021001 nanoscience & nanotechnology ,Hepatitis B ,0104 chemical sciences ,Förster resonance energy transfer ,biology.protein ,Antibody ,Human hepatitis ,0210 nano-technology ,Peptides ,Biotechnology - Abstract
Herein, we present switching-peptides for a one-step immunoassay, without the need for additional antibody treatment or washing steps to detect antigen–antibody interactions. Fluorescently labeled switching-peptides were dissociated from the immobilized antibody soon after the antigens were bound to the binding pockets. In this study, four different parts of the antibody (IgG) frame regions were chemically synthesized, and these peptides were bound to immobilized antibodies as switching-peptides. We presented the design principle of switching-peptides and used Pymol software, based on the changes in thermodynamic parameters, to study the interaction between antibodies and switching-peptides. The binding properties of switching-peptides were analyzed based on Forster resonance energy transfer between switching-peptides as well as between switching-peptides and antibodies (IgGs) isolated from different animals. The binding constants of the four switching-peptides to antibodies were estimated to be in the range of 1.48–3.29 μM. Finally, the feasibility of using switching-peptides for the quantitative one-step immunoassay was demonstrated by human hepatitis B surface antigen (hHBsAg) detection and statistical comparison of the assay results with those of conventional ELISA. The limit of detection for HBsAg was determined to be 56 ng/mL, and the dynamic range was estimated to be 136 ng/mL–33 μg/mL. These results demonstrate the feasibility of the one-step immunoassay for HBsAg.
- Published
- 2020
42. System Design for Automation in Multi-Agent-Based Manufacturing Systems
- Author
-
Samyeul Noh and Jun-Hee Park
- Subjects
0209 industrial biotechnology ,Inverse kinematics ,Computer science ,020208 electrical & electronic engineering ,Control engineering ,02 engineering and technology ,Workspace ,Task (project management) ,020901 industrial engineering & automation ,0202 electrical engineering, electronic engineering, information engineering ,Task analysis ,Robot ,Systems design ,Motion planning ,Robotic arm - Abstract
This paper proposes a system design for automation in multi-agent-based manufacturing systems to conduct a given complex task automatically by controlling multiple robotic manipulators in a systematic manner. To this end, the proposed system is designed with three-module configurations: environmental perception, task planning, and motion planning. The environmental perception module utilizes a vision sensor to recognize all objects placed on the workspace and extract their unique ID, size, and pose. The task planning module divides a given task into primitive skill levels and distributes each primitive skill to the associated robotic manipulator with the relevant object information in a systematic manner for robotic manipulators not to collide with each other. The motion planning module determines the motion of a robotic arm and a robotic hand by solving inverse kinematics for the robotic arm and by opening or closing two fingers. The proposed system has been tested and verified in real robot environments through a complex task "peg in hole" that requires at least two robotic manipulators.
- Published
- 2020
43. Transforming growth factor-β-regulated fractalkine as a marker of erosive bone invasion in oral squamous cell carcinoma
- Author
-
Seung Hwa Son, Hyungkeun Kim, Sun Kyoung Lee, Kwang Kyun Park, Jun-Hee Park, Min Ju Jung, Ki Rim Kim, and Won Yoon Chung
- Subjects
0206 medical engineering ,Cell ,Calvaria ,02 engineering and technology ,Transforming Growth Factor beta1 ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Transforming Growth Factor beta ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Secretion ,Neoplasm Invasiveness ,CX3CL1 ,General Dentistry ,Gene knockdown ,biology ,business.industry ,Chemokine CX3CL1 ,Squamous Cell Carcinoma of Head and Neck ,030206 dentistry ,020601 biomedical engineering ,stomatognathic diseases ,medicine.anatomical_structure ,Cell culture ,Head and Neck Neoplasms ,Cancer research ,biology.protein ,Carcinoma, Squamous Cell ,Mouth Neoplasms ,Antibody ,business ,Biomarkers ,Transforming growth factor - Abstract
Patients with oral squamous cell carcinoma (OSCC) bone invasion are surgically treated with bone resection, which results in severe physical and psychological damage. Here, we investigated the potential of fractalkine (CX3CL1), which is regulated by transforming growth factor (TGF-β), as a novel biomarker for correct prediction and early detection of OSCC-associated bone invasion. TGF-β knockdown and treatment with a TGF-β-neutralizing antibody decreased the level of fractalkine in the culture media of HSC-2 and YD10B OSCC cells. Treatment with a fractalkine-neutralizing antibody reduced TGF-β-stimulated invasion by HSC-2 and YD10B cells. Fractalkine treatment increased the viability, invasion, and uPA secretion of both OSCC cell lines. Furthermore, OSCC cell bone invasion was assessed following subcutaneous inoculation of wild-type or TGF-β knockdown OSCC cells in mouse calvaria. TGF-β knockdown prevented erosive bone invasion, reduced the number of osteoclasts at the tumor-bone interface, and downregulated fractalkine expression in mouse tumor tissues. Our results indicate that the production of fractalkine is stimulated by TGF-β and mediates TGF-β-induced cell invasion in several OSCC cell lines showing an erosive pattern of bone invasion. Fractalkine may be a useful predictive marker and therapeutic target for OSCC-induced bone destruction.
- Published
- 2020
44. Diagnosis of severe sepsis using phospholipids enzymatic assay based on cyclic voltammetry
- Author
-
Kwang Hwan Park, Jae Chul Pyun, Zhiquan Song, Ga Yeon Lee, Jong Min Park, Nam Su Ku, Jeong Soo Sung, Min Jung Kang, Eun Hye Lee, Sang-Guk Lee, Moo Suk Park, and Jun Hee Park
- Subjects
0106 biological sciences ,0301 basic medicine ,Double-layer capacitance ,Phospholipid ,Optical density measurement ,Bioengineering ,Electrochemistry ,01 natural sciences ,Applied Microbiology and Biotechnology ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,010608 biotechnology ,Sepsis ,Humans ,Electrodes ,Severe sepsis ,Phospholipids ,Enzyme Assays ,chemistry.chemical_classification ,Chromatography ,Chemistry ,Electrochemical Techniques ,030104 developmental biology ,Enzyme ,Graphite ,Cyclic voltammetry ,Biotechnology ,Electrochemical window - Abstract
In this work phospholipid quantification was carried out using an enzymatic assay based on cyclic voltammetry of the condensation product of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt (DAOS) and 4-aminoantipyrine (4-AP) with a graphite electrode. For the optimization of electrochemical measurement for the product, electrochemical properties such as the electrochemical window, double layer capacitance (Cdl) and electron transfer rate (kapp) were analyzed for a graphite-electrode and Au-electrode. The phospholipid enzymatic assay based the on electrochemical measurement using the graphite electrode was applied to the diagnosis of sepsis for sera from healthy volunteers (n = 16), patients with systemic inflammatory response syndrome (SIRS, n = 16) and severe sepsis patients (n = 24). Finally, the phospholipid quantification results from the electrochemical measurement were statistically compared with the conventional method based on optical density measurement.
- Published
- 2020
45. Octave bandwidth photonic fishnet-achromatic-metalens
- Author
-
Boubacar Kante, Liyi Hsu, Jun-Hee Park, Connie J. Chang-Hasnain, Abdoulaye Ndao, and Jeongho Ha
- Subjects
Infrared ,Science ,General Physics and Astronomy ,Physics::Optics ,02 engineering and technology ,01 natural sciences ,General Biochemistry, Genetics and Molecular Biology ,Article ,law.invention ,010309 optics ,Planar ,Optics ,law ,0103 physical sciences ,Broadband ,Chromatic aberration ,lcsh:Science ,Physics ,Nanophotonics and plasmonics ,Multidisciplinary ,business.industry ,General Chemistry ,021001 nanoscience & nanotechnology ,Polarization (waves) ,Achromatic lens ,Metamaterials ,lcsh:Q ,Photonics ,0210 nano-technology ,business ,Energy harvesting ,Sub-wavelength optics - Abstract
Planar structured interfaces, also known as metasurfaces, are continuously attracting interest owing to their ability to manipulate fundamental attributes of light, including angular momentum, phase, or polarization. However, chromatic aberration, limiting broadband operation, has remained a challenge for metasurfaces-based optical components and imagers. The limitation stems from the intrinsic dispersion of existing materials and design principles. Here we report and experimentally demonstrate polarization-independent fishnet-achromatic-metalenses with measured average efficiencies over 70% in the continuous band from the visible (640 nm) to the infrared (1200 nm). Results of the scalable platform are enabling for applications requiring broad bandwidth and high efficiency including energy harvesting, virtual reality and information processing devices, or medical imaging., Here the authors demonstrate all-dielectric fishnet-achromatic-metalenses from the visible to the near-infrared region. This metalens performs efficiently independent of polarization over about an octave from 640 nm to 1200 nm.
- Published
- 2020
46. Observation of plasmonic exceptional points and attomolar immuno-assay nanosensing (Conference Presentation)
- Author
-
Boubacar Kante, Jun-Hee Park, and Abdoulaye Ndao
- Subjects
Coupling ,Physics ,Singularity ,Exceptional point ,business.industry ,Physics::Optics ,Optoelectronics ,business ,Plasmon ,Immuno assay - Abstract
We have demonstrated the first plasmonic exceptional points (EPs) at subwavelength scale. The plasmonic EPs are based on the hybridization of detuned resonances in multilayered plasmonic crystals to reach a critical complex coupling rate between nanoantennas arrays, and, exhibit the dispersion of exceptional points around the non-Hermitian singularity and enhanced nanosensing was observed. The ability to drive plasmons to EPs lays the foundation to explore topological physics at small scales and to novel sensors and optoelectronic devices based on topological polaritonic effects
- Published
- 2020
47. Transcultural Adaptation and Validation of the Korean Version of the Vocal Tract Discomfort Scale
- Author
-
Jun-Hee Park, Do Yoon Jung, Ha Na Lee, Yoon Sik Park, Ji Hye Han, and Jae Yeon Yoo
- Subjects
Adult ,medicine.medical_specialty ,Voice Quality ,Audiology ,Severity of Illness Index ,Voice Disorder ,Correlation ,030507 speech-language pathology & audiology ,03 medical and health sciences ,Speech and Hearing ,Disability Evaluation ,0302 clinical medicine ,Cronbach's alpha ,Quality of life ,Surveys and Questionnaires ,Republic of Korea ,medicine ,Humans ,Prospective Studies ,Voice Handicap Index ,030223 otorhinolaryngology ,Prospective cohort study ,Reliability (statistics) ,Voice Disorders ,business.industry ,Reproducibility of Results ,LPN and LVN ,Dysphonia ,Otorhinolaryngology ,Quality of Life ,0305 other medical science ,business ,Vocal tract - Abstract
The present study translated the Vocal Tract Discomfort Scale (VTDS) into Korean (K-VTDS) and evaluated its reliability and validity.This was a prospective study.The VTDS was first translated into Korean and validated. One hundred and fifty-nine patients with voice disorders were divided into three different diagnostic groups (functional, structural, and neurologic voice disorder) and 131 vocally healthy adults were also included. All participants completed the K-VTDS and the Korean version of the Voice Handicap Index (K-VHI) and Korean versions of the Voice-Related Quality of Life (K-VRQOL). The internal consistency of the K-VTDS was analyzed through Cronbach's α coefficient. The VTDS score differences related to the diagnostic groups were assessed with t test and analysis of variance. We assessed the correlation between the K-VTDS, the K-VHI, and the K-VRQOL using Pearson's correlation analysis.High internal consistency and the test-retest reliability of the K-VTDS were found. The voice disorder group had significantly higher K-VTDS scores for the subscales and total scores than those in the healthy group (P0.001). The K-VTDS scores for the subscale of frequency and total scores were highest in the functional voice disorder group. We found a significant difference in frequency and total score of the K-VTDS between the functional voice disorder group and the structural voice disorder group (P0.05). We observed a strong positive correlation among the scores for the subscales of frequency and severity, and total scores in the K-VTDS. The K-VTDS showed moderate correlation with the K-VHI and the K-VRQOL.The K-VTDS is a reliable and valid instrument for voice assessment for voice disorders in Korean-speaking patients.
- Published
- 2020
48. Observation of plasmonic exceptional point and nano-scale sensing
- Author
-
Boubacar Kante, Abdoulaye Ndao, Jun-Hee Park, and Liyi Hsu
- Subjects
Materials science ,Exceptional point ,business.industry ,Blood circulation ,Optoelectronics ,Resonance ,Symmetry breaking ,business ,Nanoscopic scale ,Electron-beam lithography ,Plasmon ,Photonic crystal - Abstract
We propose a novel approach to EPs based on spatial symmetry breaking and report their observation in plasmonics at room temperature. The sensing of anti-Immunoglobulin G, the most common antibody found in blood circulation, is evaluated.
- Published
- 2020
49. Observation of plasmonic exceptional points at subwavelength scale
- Author
-
Boubacar Kante, Abdoulaye Ndao, Liyi Hsu, and Jun-Hee Park
- Subjects
Physics ,business.industry ,Scale (descriptive set theory) ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,010309 optics ,Resonator ,Nanosensor ,0103 physical sciences ,Optoelectronics ,Surface plasmon resonance ,0210 nano-technology ,business ,Refractive index ,Electron-beam lithography ,Plasmon ,Photonic crystal - Abstract
We propose a novel approach to EPs and report their first observation in plasmonics at room temperature. Enhanced sensing of anti-Immunoglobulin G (attomolar detection), the most common antibody found in blood circulation, is reported.
- Published
- 2020
50. The FaaS system using additive manufacturing for personalized production
- Author
-
Hyoung Seok Kang, Ji Yeon Son, Sang Do Noh, Ju Yeon Lee, Hyun Kim, and Jun-Hee Park
- Subjects
0209 industrial biotechnology ,business.industry ,Computer science ,Mechanical Engineering ,Mass customization ,3D printing ,02 engineering and technology ,Virtualization ,computer.software_genre ,Industrial and Manufacturing Engineering ,Manufacturing engineering ,Personalization ,020901 industrial engineering & automation ,0202 electrical engineering, electronic engineering, information engineering ,Numerical control ,Factory (object-oriented programming) ,020201 artificial intelligence & image processing ,Manufacturing operations ,business ,computer ,Distributed manufacturing - Abstract
Purpose In this paper, a three-dimensional (3D) printer-based manufacturing line and supporting system, which supports personalized/customized manufacturing for individual businesses or start-up companies, was studied to evaluate the practicality of using additive manufacturing for personalization/mass customization. Design/methodology/approach First, factory-as-a-service (FaaS) system, which provides factory as a service to customers, was proposed and designed to manufacture various products within a distributed manufacturing environment. This system includes 3D printer-based material extrusion processes, vapor machine/computer numerical control machines as post-processes and assembly and inspection processes with an automated material handling robot in the factory. Second, a virtualization module for the FaaS factory was developed using a simulation model interfaced with a cloud-based order and production-planning system and an internet-of-things-based control and monitoring system. This is part of the system for manufacturing operations, which is capable of dynamic scheduling in a distributed manufacturing environment. In addition, simulation-based virtual production was conducted to verify and evaluate the FaaS factory for the target production scenario. Main information of the simulation also has been identified and included in the virtualization module. Finally, the established system was applied in a sample production scenario to evaluate its practicality and efficiency. Findings Additive manufacturing is a reliable, feasible and applicable technology, and it can be a core element in smart manufacturing and the realization of personalization/mass customization. Originality/value Various studies on additive manufacturing have been conducted with regard to replacing the existing manufacturing methods or integrating with them, but these studies mostly focused on materials or types of additive manufacturing, with few advanced or applied studies on the establishment of a new manufacturing environment for personalization/mass customization.
- Published
- 2018
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