10 results on '"Li-Chuan Liao"'
Search Results
2. Targeting the VEGF-C/VEGFR3 axis suppresses Slug-mediated cancer metastasis and stemness via inhibition of KRAS/YAP1 signaling
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Chien Ping Chiang, Sin Ying Tsai, Earl Fu, Yung Luen Yu, Yu Wen Yeh, Pei Wen Tsai, Li Chuan Liao, Ting Yu Chang, Chi Feng Tseng, Shu Ting Yang, Jen Liang Su, and Ching Chia Cheng
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cancer stemness ,0301 basic medicine ,Oncology ,Gerontology ,medicine.medical_specialty ,Skin Neoplasms ,Slug ,Vascular Endothelial Growth Factor C ,VEGF-C ,YAP1 ,Review ,medicine.disease_cause ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Cell Movement ,Internal medicine ,Humans ,metastasis ,Medicine ,Neoplasm Invasiveness ,skin cancer ,biology ,business.industry ,Cancer ,Vascular Endothelial Growth Factor Receptor-3 ,medicine.disease ,biology.organism_classification ,Molecular medicine ,030104 developmental biology ,030220 oncology & carcinogenesis ,Neoplastic Stem Cells ,Snail Family Transcription Factors ,KRAS ,Skin cancer ,business ,Signal Transduction ,Biomedical sciences - Abstract
// Yu-Wen Yeh 1, 2 , Ching-Chia Cheng 3, 4 , Shu-Ting Yang 4 , Chi-Feng Tseng 4 , Ting-Yu Chang 4 , Sin-Ying Tsai 5 , Earl Fu 6 , Chien-Ping Chiang 7 , Li-Chuan Liao 8 , Pei-Wen Tsai 9 , Yung-Luen Yu 5, 10, 11 , Jen-Liang Su 4, 5, 10, 11 1 Division of Dermatology, Tri-Service General Hospital Songshan Branch, National Defense Medical Center, Taipei 10581, Taiwan 2 Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei 11490, Taiwan 3 Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 11490, Taiwan 4 National Institute of Cancer Research, National Health Research Institutes, Zhunan, Miaoli County 35053, Taiwan 5 Department of Biotechnology, Asia University, Taichung 41354, Taiwan 6 Department of Periodontology, Tri-Service General Hospital, National Defense Medical Center, Taipei 11490, Taiwan 7 Department of Dermatology, Tri-Service General Hospital, National Defense Medical Center, Taipei 11490, Taiwan 8 Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 30062, Taiwan 9 Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu 30013, Taiwan 10 Graduate Institute of Biomedical Sciences, China Medical University, Taichung 40402, Taiwan 11 Center for Molecular Medicine, China Medical University Hospital, Taichung 40447, Taiwan Correspondence to: Jen-Liang Su, email: jlsu@nhri.org.tw Yung-Luen Yu, email: ylyu@mail.cmu.edu.tw Keywords: VEGF-C, YAP1, metastasis, cancer stemness, skin cancer Received: August 08, 2016 Accepted: November 07, 2016 Published: November 25, 2016 ABSTRACT Vascular endothelial growth factor-C (VEGF-C) has been implicated in epithelial-mesenchymal transition (EMT) processes and various human cancers, including skin cancer. Skin cancer is an aggressive human malignancy with increasing incidence worldwide; however, the underlying mechanisms involved in VEGF-C-induced skin cancer stemness and metastasis remain unclear. Here, we report that VEGF-C enhances skin cancer migration, invasion and stemness through Slug up-regulation. Oncomine database analysis indicated that the KRAS/MAPK (mitogen-activated protein kinases) pathway and YAP1 (yes-associated protein 1) expression are positively correlated with metastatic skin cancer. We show that VEGF-C triggers the activation of KRAS/MAPK signaling to increase YAP1 and downstream Slug expression, which are suppressed by an anti-VEGFR3 (VEGF receptor 3) peptide, a specific peptide targeting VEGFR3. The VEGF-C-induced migration, invasion and stemness of skin cancer cells are also abrogated by the anti-VEGFR3 peptide. Based on these data, we reveal the role of the VEGF-C/VEGFR3-mediated KRAS/MAPK-YAP1/Slug pathway in skin cancer progression and propose that the VEGF-C/VEGFR3 axis is a promising target for the anti-VEGFR3 peptide.
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- 2016
3. Fluorescent Fe Embedded Magnetic Nanodiamonds Made by Ion Implantation
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Hung-Kai Yu, Tung-Yuan Hsiao, Li-Chuan Liao, C. P. Lee, Huan Niu, Yu-Jen Chang, Srinivasu Kunuku, Tzung-Yuang Chen, Chien-Hsu Chen, and Bo-Rong Lin
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0301 basic medicine ,Multidisciplinary ,Materials science ,Biocompatibility ,Annealing (metallurgy) ,lcsh:R ,lcsh:Medicine ,Diamond ,Nanoparticle ,Nanotechnology ,engineering.material ,equipment and supplies ,Fluorescence ,Article ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Ion implantation ,engineering ,lcsh:Q ,Graphite ,Surface layer ,lcsh:Science ,human activities ,030217 neurology & neurosurgery - Abstract
We demonstrate fluorescent Fe embedded magnetic nanodiamonds by ion implantation and two-step annealing. The diamond characteristics with a highly ordered core and a graphite surface layer are maintained after the implantation process. After the two-step annealing process, a bright red fluorescence associated with nitrogen-vacancy centers is observed. These new fluorescent magnetic nanodiamonds can be used as a dual-function in vivo tracer with both optical visibility and magnetic resonance imaging capabilities. They are potentially useful for the more advanced in vivo biological and medical applications.
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- 2019
4. Iron Embedded Magnetic Nanodiamonds for in vivo MRI Contrast Enhancement
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C. P. Lee, Huan Niu, Tzung-Yuang Chen, Chun-Hsiang Chang, Fang-Hsin Chen, Chien-Hsu Chen, Srinivasu Kunuku, Yu-Jen Chang, Li Chuan Liao, Bo-Rong Lin, Hung-Kai Yu, and Tung-Yuan Hsiao
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Materials science ,Contrast enhancement ,Acoustics and Ultrasonics ,FOS: Physical sciences ,02 engineering and technology ,Image enhancement ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,Physics - Medical Physics ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,In vivo ,Biological Physics (physics.bio-ph) ,Drug delivery ,Medical imaging ,Physics - Biological Physics ,Medical Physics (physics.med-ph) ,Mr images ,0210 nano-technology ,Biomedical engineering - Abstract
Although nanodiamonds have long being considered as a potential tool for biomedical research, the practical in vivo application of nanodiamonds remains relatively unexplored. In this paper, we present the first application of in vivo MRI contrast enhancement using only iron embedded magnetic nanodiamonds. MR image enhancement was clearly demonstrated in the rendering of T2-weighted images of mice obtained using an unmodified commercial MRI scanner. The excellent contrast obtained using these nanodiamonds opens the door to the non-invasive in vivo tracking of NDs and image enhancement. In the future, one can apply these magnetic nanodiamonds together with surface modifications to facilitate drug delivery, targeted therapy, localized thermal treatment, and diagnostic imaging., Comment: 19 pages, 6 figures
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- 2019
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5. Secondary ion mass spectrometry to verify the implantation of magnetic ions in nanodiamonds
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Bo-Rong Lin, Chun-Hsiang Chang, Srinivasu Kunuku, Chien-Hsu Chen, Hung-Kai Yu, Tung-Yuan Hsiao, Li-Chuan Liao, Fang-Hsin Chen, C. P. Lee, Chiung-Chi Wang, Yu-Jen Chang, Tzung-Yuang Chen, and Huan Niu
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010302 applied physics ,Range (particle radiation) ,Materials science ,General Physics and Astronomy ,Nanoparticle ,Nanotechnology ,02 engineering and technology ,engineering.material ,equipment and supplies ,021001 nanoscience & nanotechnology ,01 natural sciences ,Ion ,Secondary ion mass spectrometry ,Ion implantation ,Coating ,0103 physical sciences ,engineering ,Surface modification ,Wafer ,0210 nano-technology ,human activities - Abstract
Ion implantation is used to create nanodiamonds (NDs) with embedded magnetic ions for use in a wide range of biological and medical applications; however, the effectiveness of this process depends heavily on separating magnetic NDs from nonmagnetic ones. In this study, we use secondary ion mass spectrometry to verify the implantation of magnetic ions in NDs and the success of separation. When applied to a series of NDs with embedded iron or manganese ions, the sorting tool used in this study proved highly effective in selecting magnetic NDs. Besides, multienergy ion implantation and precise thickness control of NDs coating on the silicon wafer were suggested to improve this technology.
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- 2019
6. A Synthetic Peptide-Acrylate Surface for Production of Insulin-Producing Cells from Human Embryonic Stem Cells
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Yao-Chen Yang, Shih-Han Hung, I-Ming Chu, Pei-Yi Lin, Li-Chuan Liao, Hsan-Jan Yen, Yi-Cheng Hsieh, Huai-En Lu, Shiaw-Min Hwang, and Maw-Sheng Lee
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KOSR ,Surface Properties ,Cellular differentiation ,Cell Culture Techniques ,Biocompatible Materials ,Germ layer ,Biology ,Original Research Reports ,Humans ,Insulin ,Vitronectin ,Cells, Cultured ,Embryonic Stem Cells ,Matrigel ,Molecular Mimicry ,Cell Differentiation ,Cell Biology ,Hematology ,Molecular biology ,Embryonic stem cell ,Peptide Fragments ,Culture Media ,Drug Combinations ,Acrylates ,Cell culture ,Proteoglycans ,Collagen ,Laminin ,Subculture (biology) ,Stem cell ,Developmental Biology - Abstract
Human embryonic stem cells (hESCs), due to their self-renewal capacity and pluripotency, have become a potential source of transplantable β-cells for the treatment of diabetes. However, it is imperative that the derived cells fulfill the criteria for clinical treatment. In this study, we replaced common Matrigel with a synthetic peptide-acrylate surface (Synthemax) to expand undifferentiated hESCs and direct their differentiation in a defined and serum-free medium. We confirmed that the cells still expressed pluripotent markers, had the ability to differentiate into three germ layers, and maintained a normal karyotype after 10 passages of subculture. Next, we reported an efficient protocol for deriving nearly 86% definitive endoderm cells from hESCs under serum-free conditions. Moreover, we were able to obtain insulin-producing cells within 21 days following a simple three-step protocol. The results of immunocytochemical and quantitative gene expression analysis showed that the efficiency of induction was not significantly different between the Synthemax surface and the Matrigel-coated surface. Thus, we provided a totally defined condition from hESC culture to insulin-producing cell differentiation, and the derived cells could be a therapeutic resource for diabetic patients in the future.
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- 2014
7. Fluorescent 10B embedded nanodiamonds as 10B delivery agent for boron neutron capture therapy
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Bo-Rong Lin, Srinivasu Kunuku, Tzung-Yuang Chen, Yu-Jen Chang, Li-Chuan Liao, Chien-Hsu Chen, Tung-Yuan Hsiao, Hung-Kai Yu, Huan Niu, and Chien-Ping Lee
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inorganic chemicals ,Boron Delivery Agent ,Materials science ,Acoustics and Ultrasonics ,Annealing (metallurgy) ,chemistry.chemical_element ,Nanotechnology ,02 engineering and technology ,Isotopes of boron ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Fluorescence ,Neutron therapy ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,03 medical and health sciences ,Neutron capture ,0302 clinical medicine ,Ion implantation ,chemistry ,030220 oncology & carcinogenesis ,0210 nano-technology ,Boron - Abstract
Boron neutron capture therapy is a powerful anti-cancer treatment, the success of which depends heavily on the boron delivery agent. Enabling the real-time tracing of delivery agents as they move through the body is crucial to the further development of boron neutron therapy. In this study, we fabricate highly bio-compatible boron-10 embedded nanodiamonds using physical ion implantation in conjunction with a two-step annealing process. The red fluorescence of the nanodiamonds allows their use in fluorescence microscopy and in vivo imaging systems, thereby making it possible to conduct tracking in real time. The proposed fluorescent boron-10 embedded nanodiamonds, combining optical visibility and boron-10 transport capability, are a promising boron delivery agent suitable for a wide range of biomedical applications.
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- 2019
8. Anchoring effect on foreign institutional investors’ momentum trading behavior: Evidence from the Taiwan stock market
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Ray Yeutien Chou, Banghan Chiu, and Li-Chuan Liao
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Economics and Econometrics ,Momentum (finance) ,Foreign ownership ,Institutional investor ,Anchoring ,Financial system ,Stock market ,Profitability index ,Business ,Market value ,Finance - Abstract
On Taiwan's stock market, foreign institutional investors hold over one-third of the total market value and have enjoyed remarkable returns on their investments. Hence, it is important to investigate the trading behavior of foreign institutional investors. Previous studies have found that foreign institutional investors are momentum traders. This study documents a cognitive bias – anchoring effect – on foreign institutional investors’ trading decisions. Moreover, we document that foreign institutional investors’ momentum behavior is influenced (anchored) by prior foreign ownership (anchor). We also show that foreign investors’ momentum behavior is strengthened when prior foreign ownership is high. However, the anchoring effect cannot improve momentum profitability. In some cases, momentum profitability suffers because of the anchoring effect.
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- 2013
9. Highly efficient drug delivery systems based on functional supramolecular polymers: In vitro evaluation
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Jem-Kun Chen, Duu-Jong Lee, Chih-Chia Cheng, Shiaw Min Hwang, Wan Yi Kao, Yu Jen Chang, Mei-Chih Liang, Li Chuan Liao, and Feng-Chih Chang
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Drug ,Materials science ,Polymers ,media_common.quotation_subject ,Biomedical Engineering ,Supramolecular chemistry ,Nanotechnology ,Biocompatible Materials ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Micelle ,Biomaterials ,Drug Delivery Systems ,Humans ,Scattering, Radiation ,Thermoresponsive polymers in chromatography ,Molecular Biology ,Micelles ,media_common ,chemistry.chemical_classification ,Temperature ,General Medicine ,Polymer ,Hep G2 Cells ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Supramolecular polymers ,HEK293 Cells ,chemistry ,Drug delivery ,Nanocarriers ,0210 nano-technology ,Biotechnology - Abstract
The novel concept of modifying and enhancing the properties of existing functional micelles through self-complementary interactions has significant potential. In this study, a practical approach to living polymerization of functionalized thermoresponsive monomers enabled the incorporation of self-constituted multiple hydrogen bonded groups into micelles that have potential as supramolecular drug-delivery systems. Phase transitions and morphological studies in aqueous solution showed that the microstructure can be controlled to achieve well-defined vesicle-like micelles with respect to the strength of the hydrogen bond segment. Thus, the resulting micelles have a very low critical micellization concentration and very high loading capacity (16.1%), making the loading process extremely stable and efficient. Incorporation of the anticancer drug doxorubicin (DOX) affected the micellization process in aqueous solution and enabled fine-tuning of drug loading and precise control of drug release rate with excellent sensitivity. Release studies in vitro showed that DOX-loaded micelles exerted dose-dependent cytotoxicity against human liver carcinoma (HepG2) cells at the physiological temperature of 37 °C. In addition, DOX-loaded micelles were efficiently endocytosed by the cancer cells, which may enable the micelles to serve as suitable vehicles for effective delivery of anticancer drugs to primary tumors and metastatic disease. This newly developed material may provide a potential route towards next-generation drug delivery vehicles. Statement of Significance A breakthrough innovation in water-based thermo-responsive polymers has enabled significant progress in developing smart stimuli-responsive nanocarriers by generating novel “supramolecular polymeric micelles” via self-complementary hydrogen-bonding interactions. These newly developed micelles exhibit extremely high micellar stability and drug loading capacity (up to 16%), excellent thermo-responsive behavior and precise control of drug release rate due to hydrogen-bond-induced physical cross-linking. In addition, doxorubicin-loaded micelles were efficiently endocytosed by the cancer cells, which allows them to serve as suitable vehicles for effective delivery of anticancer drugs to primary tumors and metastatic disease. Thus, this work provides a potential route for the development of next generation multifunctional nanocarriers that have improved safety and to increase the therapeutic efficacy of anticancer therapy.
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- 2015
10. A 2-yr service report of cell line authentication
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Shin-Ying Lee, Li-Chuan Liao, Shiaw-Min Hwang, Chiung-Yun Chen, Mei-Ling Wu, and Gwo-Fang Yuan
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Quality Control ,Service (business) ,Challenge-Handshake Authentication Protocol ,business.industry ,Computer science ,Cell Culture Techniques ,Taiwan ,Biological Specimen Banks ,Cell Biology ,General Medicine ,Authentication (law) ,Cell Line ,Cell culture ,Authentication protocol ,Humans ,Periodicals as Topic ,business ,Editorial Policies ,Microsatellite Repeats ,Developmental Biology ,Computer network - Published
- 2013
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