16 results on '"protective agent"'
Search Results
2. Current guidelines for the management of non-alcoholic fatty liver disease: A systematic review with comparative analysis
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Simona Leoni, Francesco Tovoli, Lucia Napoli, S. Ferri, Ilaria Serio, Luigi Bolondi, Leoni, Simona, Tovoli, Francesco, Napoli, Lucia, Serio, Ilaria, Ferri, Silvia, and Bolondi, Luigi
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0301 basic medicine ,Fibrosi ,Biopsy ,Bariatric Surgery ,Disease ,Global Health ,Chronic liver disease ,0302 clinical medicine ,Non-alcoholic Fatty Liver Disease ,Prevalence ,Ultrasonography ,Evidence-Based Medicine ,Non-invasive diagnosi ,Liver Function Test ,Fatty liver ,Gastroenterology ,General Medicine ,Liver biopsy ,Magnetic Resonance Imaging ,Metformin ,Liver ,Liver steatosi ,Practice Guidelines as Topic ,030211 gastroenterology & hepatology ,Human ,medicine.medical_specialty ,Alcohol Drinking ,digestive system ,03 medical and health sciences ,Internal medicine ,medicine ,Obesity ,Intensive care medicine ,Clinical guideline ,Protective Agent ,Pioglitazone ,Hypoglycemic Agent ,business.industry ,Risk Factor ,Significant difference ,Metabolic risk ,nutritional and metabolic diseases ,Non alcoholic ,Hepatology ,medicine.disease ,digestive system diseases ,Liver Transplantation ,030104 developmental biology ,Diabetes Mellitus, Type 2 ,business ,Dyslipidemia - Abstract
The current epidemic of non-alcoholic fatty liver disease (NAFLD) is reshaping the field of hepatology all around the world. The widespread diffusion of metabolic risk factors such as obesity, type2-diabetes mellitus, and dyslipidemia has led to a worldwide diffusion of NAFLD. In parallel to the increased availability of effective anti-viral agents, NAFLD is rapidly becoming the most common cause of chronic liver disease in Western Countries, and a similar trend is expected in Eastern Countries in the next years. This epidemic and its consequences have prompted experts from all over the word in identifying effective strategies for the diagnosis, management, and treatment of NAFLD. Different scientific societies from Europe, America, and Asia-Pacific regions have proposed guidelines based on the most recent evidence about NAFLD. These guidelines are consistent with the key elements in the management of NAFLD, but still, show significant difference about some critical points. We reviewed the current literature in English language to identify the most recent scientific guidelines about NAFLD with the aim to find and critically analyse the main differences. We distinguished guidelines from 5 different scientific societies whose reputation is worldwide recognised and who are representative of the clinical practice in different geographical regions. Differences were noted in: the definition of NAFLD, the opportunity of NAFLD screening in high-risk patients, the non-invasive test proposed for the diagnosis of NAFLD and the identification of NAFLD patients with advanced fibrosis, in the follow-up protocols and, finally, in the treatment strategy (especially in the proposed pharmacological management). These difference have been discussed in the light of the possible evolution of the scenario of NAFLD in the next years.
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- 2018
3. Enhancement of viability, acid, and bile tolerance and accelerated stability in lyophilized
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Mina, Kim, Dong-Geon, Nam, Sang-Bum, Kim, Pureum, Im, Jeong-Sook, Choe, and Ae-Jin, Choi
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lactic acid bacteria ,freeze‐drying ,accelerated storage ,Weissella ,food and beverages ,probiotic ,Original Research ,protective agent - Abstract
Dietary supplementation with lactic acid bacteria to maintain or improve intestinal health is advocated. Weissella spp. are present in different fermented vegetable‐based foods like kimchi, as well as in the normal gastrointestinal (GI) tract of humans. Weissella cibaria strains have been proposed as potential probiotics. Freeze‐drying is a promising treatment method for these strains for industrial applications and to increase the accessibility of their health‐promoting benefits. Moreover, probiotic strains need to be able to survive in the host GI tract, and acid and bile are both environmental stressors that can reduce strain survival. Therefore, this study evaluated the effect of the combination of protective agents on the acid and bile resistance of W. cibaria JW15 after freeze‐drying. A protective agent combination with a 1:1 ratio of 5 g + 5 g/100 ml w/v soy flour + yeast extract (SFY) retained nearly 100% viability after freeze‐drying and was resistant to artificial bile acids. Remarkably, skim milk + soy flour (SSF) was resistant to an acidic solution, and the viability of W. cibaria JW15 in artificial gastric acid was enhanced when treated with this mixture. Furthermore, SFY and SSF were found to maintain high numbers of viable cells with a low specific rate of cell death (k) after storage at 50°C, 60°C, and 70°C. These results support an effective probiotic formulation system with a high number of viable cells, and its protective effects can be leveraged in the development of probiotic products with health benefits.
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- 2018
4. [Application of pegylated recombinant human granulocyte colony-stimulating factor to prevent chemotherapy-induced neutropenia in patients with lymphoma: a prospective, multicenter, open-label clinical trial]
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H Q, Huang, B, Bai, Y H, Gao, D H, Zou, S H, Zou, H, Tan, Y P, Song, Z Y, Li, J, Jin, W, Li, H, Su, Y P, Gong, M Z, Zhong, Y R, Shuang, J, Zhu, J Q, Zhang, Z, Cai, Q L, Teng, W J, Sun, Y, Yang, Z J, Xia, H L, Chen, L M, Hua, Y Y, Bao, and N, Wu
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聚乙烯二醇类 ,Lung Neoplasms ,Neutropenia ,Lymphoma ,Polyethylene glycols ,预防和防护用药 ,Recombinant Proteins ,论著 ,Granulocyte colony-stimulating factor ,中性粒细胞减少 ,粒细胞集落刺激因子 ,Antineoplastic Combined Chemotherapy Protocols ,Humans ,淋巴瘤 ,Prospective Studies ,Neoplasm Recurrence, Local ,Protective agent - Abstract
目的 评价聚乙二醇化重组人粒细胞刺激因子(PEG-rhG-CSF)预防淋巴瘤患者化疗后发生中性粒细胞减少症的有效性和安全性。 方法 本研究为多中心、开放、单臂、Ⅳ期临床试验。纳入410例淋巴瘤患者接受多周期化疗并预防性使用PEG-rhG-CSF。主要观察患者各化疗周期Ⅲ/Ⅳ度中性粒细胞减少症和发热性中性粒细胞减少(FN)的发生率,同时观察患者整个化疗期间抗生素的使用率。 结果 ①410例患者中,违背入选标准8例(1.95%),失访35例(8.54%),发生不良事件19例(4.63%),出现符合终止研究标准者12例(2.93%),疾病进展或复发15例(3.66%),故最终321例(78.29%)进入符合方案集。②在第1~4个治疗周期,初级预防给予PEG-rhG-CSF后,Ⅳ度中性粒细胞减少症的发生率分别为19.14%(49/256)、12.50%(32/256)、12.18%(24/197)、13.61%(20/147),FN的发生率分别为3.52%(9/256)、0.39%(1/256)、2.54%(5/197)、2.04%(3/147);次级预防给药后,Ⅳ度中性粒细胞减少症的发生率从61.54%(40/65)降至16.92%(11/65)、18.46%(12/65)、20.75%(11/53),FN的发生率从16.92%(11/65)降至1.54%(1/65)、4.62%(3/65)、3.77%(2/53)。③整个化疗期间接受抗生素治疗的受试者比例为34.39%(141/410)。④与PEG-rhG-CSF相关的不良事件发生率为4.63%(19/410),最常见的不良反应为骨痛[3.90%(16/410)]、乏力(0.49%)和发热(0.24%)。 结论 在淋巴瘤患者化疗过程中,预防性使用PEG-rhG-CSF能够有效降低化疗过程中Ⅲ/Ⅳ度中性粒细胞减少症和FN的发生率,确保淋巴瘤患者接受标准剂量化疗,提高治愈率。
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- 2017
5. Protective effects of diltiazem and tadalafil on shock wave-induced kidney injury in rats
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O Kaygisiz, H Vuruskan, G Ozmerdiven, B A Vuruskan, Uludağ Üniversitesi/Tıp Fakültesi/Üroloji Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı., Özmerdiven, Gökhun, Vuruşkan, Berna Aytaç, Kaygısız, Onur, Vuruşkan, Hakan, AAH-9746-2021, AAM-9726-2020, and L-9439-2019
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Male ,medicine.medical_treatment ,Kidney Glomerulus ,Ischemia/reperfusion injury ,030204 cardiovascular system & hematology ,Nephrectomy ,Tadalafil ,Rats, Sprague-Dawley ,Tubular dysfunction ,Diltiazem ,0302 clinical medicine ,Lithotripsy ,Mechanisms ,Pathology ,Materials Chemistry ,Renal tubules ,Injuries ,Heat shock protein ,Ultrasonic Lithotripsy ,Shock Waves ,Urolithiasis ,05 social sciences ,Forestry ,Acute Kidney Injury ,Medicine, general & internal ,Sprague dawley rat ,Combination drug therapy ,Kidney Tubules ,Shock (circulatory) ,Reperfusion Injury ,Drug Therapy, Combination ,Renal ischemia-reperfusion ,medicine.symptom ,medicine.drug ,Economics and Econometrics ,medicine.medical_specialty ,Nifedipine ,Allopurinol ,Ischemia ,Urology ,Ischemia-reperfusion injury ,Protective Agents ,03 medical and health sciences ,0502 economics and business ,Media Technology ,medicine ,Animals ,Cell damage ,Pig ,Kidney tubule ,Drug effects ,Adverse effects ,Animal ,urogenital system ,business.industry ,Shock wave lithotripsy ,medicine.disease ,Rats ,Verapamil ,Glomerulus ,Rat ,Calcium ,050211 marketing ,General & internal medicine ,Protective agent ,business ,Reperfusion injury - Abstract
Background We aimed to compare the protective effects of tadalafil and diltiazem on renal histology after ischemia and reperfusion injury in a rat model of shock wave lithotripsy. Methods A total of 40 adult, male Sprague-Dawley rats were randomized into four groups as follows; control group (group C), group S (SWL + nephrectomy), group T (SWL + tadalafil given before nephrectomy) and group D (SWL + diltiazem given before nephrectomy). Both kidneys were evaluated regarding tubular damage, peritubular fibrosis and heat shock protein-70 (HSP-70) immune-expression of glomeruli, cortical and medullar collector tubules on light microscopy. Results HSP-70 levels of cortical and medullar collector tubules, tubular damage and peritubular fibrosis scores were decreased in group T compared with group S. Similarly, HSP-70 immunostaining levels on cortical and medullar collector tubules, tubular damage and peritubular fibrosis scores were decreased in group D compared with group S. No significant difference was detected between group D and group T for all parameters. Conclusion As a result, shock waves induced renal cell damage due to increment of HSP-70 levels, morphological irregularity in tubules and increased peritubular fibrosis. Tadalafil and diltiazem had beneficial effects in decreasing renal tissue damage which was caused by SWL (Tab. 2, Fig. 6, Ref. 29).
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- 2017
6. Preventive Strategies for Contrast-Induced Acute Kidney Injury in Patients Undergoing Percutaneous Coronary Procedures: Evidence From a Hierarchical Bayesian Network Meta-Analysis of 124 Trials and 28 240 Patients
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Roxana Mehran, Davide Capodanno, Patrizia Aruta, Corrado Tamburino, Salvatore Cassese, Adnan Kastrati, Giuseppe Gargiulo, Robert A. Byrne, Daniele Giacoppo, George Dangas, Sergio Buccheri, Giacoppo, D., Gargiulo, G., Buccheri, S., Aruta, P., Byrne, R. A., Cassese, S., Dangas, G., Kastrati, A., Mehran, R., Tamburino, C., and Capodanno, D.
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Cardiac Catheterization ,Percutaneous ,medicine.medical_treatment ,Contrast Media ,030204 cardiovascular system & hematology ,Coronary Angiography ,Radiography, Interventional ,meta-analysi ,0302 clinical medicine ,Risk Factors ,Odds Ratio ,Medicine ,030212 general & internal medicine ,Cardiac catheterization ,Randomized Controlled Trials as Topic ,Interventional ,Acute kidney injury ,creatinine ,Acute Kidney Injury ,Markov Chains ,Treatment Outcome ,Meta-analysis ,Anesthesia ,Cardiology and Cardiovascular Medicine ,Risk assessment ,Monte Carlo Method ,Human ,medicine.medical_specialty ,Protective Agents ,Risk Assessment ,Article ,03 medical and health sciences ,Percutaneous Coronary Intervention ,acetylcysteine ,Humans ,In patient ,Intensive care medicine ,Protective Agent ,Protective Factor ,business.industry ,Risk Factor ,Percutaneous coronary intervention ,acute kidney injury ,chronic kidney disease ,contrast media ,meta-analysis ,percutaneous coronary intervention ,Bayes Theorem ,Protective Factors ,Fluid Therapy ,Odds ratio ,Markov Chain ,medicine.disease ,Radiography ,business - Abstract
Background— The effectiveness of currently available effective preventive strategies for contrast-induced acute kidney injury (CIAKI) is a matter of debate. Methods and Results— We performed a Bayesian random-effects network meta-analysis of 124 trials (28 240 patients) comparing a total of 10 strategies: saline, statin, N-acetylcysteine (NAC), sodium bicarbonate (NaHCO 3 ), NAC+NaHCO 3 , ascorbic acid, xanthine, dopaminergic agent, peripheral ischemic preconditioning, and natriuretic peptide. Compared with saline, the risk of CIAKI was reduced by using statin (odds ratio [OR], 0.42; 95% credible interval [CrI], 0.26–0.67), xanthine (OR, 0.32; 95% CrI, 0.17–0.57), ischemic preconditioning (OR, 0.48; 95% CrI, 0.26–0.87), NAC+NaHCO 3 (OR, 0.50; 95% CrI, 0.33–0.76), NAC (OR, 0.68; 95% CrI, 0.55–0.84), and NaHCO 3 (OR, 0.66; 95% CrI, 0.47–0.90). The benefit of statin therapy was consistent across multiple sensitivity analyses, whereas the efficacy of all the other strategies was questioned by restricting the analysis to high-quality trials. Overall, high heterogeneity was observed for comparisons involving xanthine and ischemic preconditioning, although the impact of NAC and xanthine was probably influenced by publication bias/small-study effect. Hydration alone was the least effective preventive strategy for CIAKI. Meta-regressions did not reveal significant associations with baseline creatinine and contrast volume. In patients with diabetes mellitus, no strategy was found to reduce the incidence of CIAKI. Conclusions— In patients undergoing percutaneous coronary procedures, statin administration is associated with a marked and consistent reduction in the risk of CIAKI compared with saline. Although xanthine, NAC, NaHCO 3 , NAC+NaHCO 3 , ischemic preconditioning, and natriuretic peptide may have nephroprotective effects, these results were not consistent across multiple sensitivity analyses.
- Published
- 2016
7. PVP Protective mechanism of palladium nanoparticles obtained by sonochemical process
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Hayet Moumeni, Jean-Luc Rehspringer, and Abderrafik Nemamcha
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Materials science ,Absorption spectroscopy ,chemistry.chemical_element ,Nanoparticle ,Physics and Astronomy(all) ,Palladium nanoparticles ,UV-Visible spectroscopy ,chemistry.chemical_compound ,Ultraviolet visible spectroscopy ,Adsorption ,chemistry ,EDX ,FT-IR spectroscopy ,MET ,Protective agent ,Fourier transform infrared spectroscopy ,High-resolution transmission electron microscopy ,Ethylene glycol ,Nuclear chemistry ,Palladium - Abstract
The protective effect of polyvinylpyrrolidone (PVP) on the palladium nanoparticles has been investigated using Fourier Transform Infrared Spectroscopy (FT-IR). Palladium nanoparticles have been obtained by ultrasonic irradiation of Pd(NO3)2 solution in presence of ethylene glycol (EG) and PVP. The sonochemical reduction process of palladium ions (Pd(II)) to palladium atoms (Pd(0)) can be explained by considering the intense ultrasonic waves that are strong enough to produce cavitation: formation, growth and collapse of bubbles. The UV-Visible absorption spectroscopy revealed that the reduction of Pd (II) to metallic Pd has been successfully achieved. The FT-IR spectroscopy spectra analysis show that, in presence of ethylene glycol, the stabilization of the nanoparticles results from the adsorption of the PVP chain on the palladium particle surface via the coordination of the PVP carbonyl group to the palladium atoms. The transmission electron microscopy (HRTEM) and electrondispersive X-ray (EDX) results confirm the dispersion, the nanometric size and the composition of the obtained palladium particles.
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- 2009
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8. Periapical tissue healing after post space preparation with or without use of a protection plug and root canal exposure to the oral environment: study in dogs
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Sueli Satomi Murata, Luciana Noronha Manne, Eloi Dezan Junior, Valdir de Souza, Roberto Holland, Universidade Estadual Paulista (Unesp), and Universidade de Marília (UNIMAR)
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Hydrocortisone ,Root canal ,Dentistry ,wound healing ,Dexamethasone ,post space preparation ,Gram positive bacterium ,Root Canal Obturation ,tooth root ,Dentin ,animal ,tooth crown ,root canal filling material ,tooth disease ,Coltosol F ,instrumentation ,Dental Cementum ,Dental Leakage ,Dental Pulp Cavity ,biology ,Periapical Tissue ,zinc oxide ,periapical repair ,methodology ,root canal therapy ,Endodontics ,unclassified drug ,Drug Combinations ,medicine.anatomical_structure ,dog ,coronal leakage ,tooth pulp ,Zinc Oxide ,Root Canal Preparation ,cementum ,Post and Core Technique ,medicine.medical_specialty ,Periodontal Ligament ,drug combination ,Gram negative bacterium ,dentin ,Gram-Positive Bacteria ,Protective Agents ,Calcium Sulfate ,Calcium Hydroxide ,Root Canal Filling Materials ,Dogs ,Tooth Apex ,corticosteroid methanetriol mixture ,thymol ,Formaldehyde ,Gram-Negative Bacteria ,medicine ,Animals ,General Dentistry ,Dental Pulp ,protective agent ,Wound Healing ,Calcibiotic Root Canal Sealer ,isolation and purification ,business.industry ,microbiology ,zinc sulfate ,root canal sealer ,Gutta-percha ,biology.organism_classification ,gutta percha ,Thymol ,Zinc Sulfate ,endodontics ,drug derivative ,physiology ,pathology ,Dental cementum ,Gutta-Percha ,business ,periodontium - Abstract
The purpose of this study was to evaluate the influence of coronal leakage on the healing of dogs' periapical tissues after root canal filling, post space preparation and protection or not with a temporary sealer plug. Forty root canals of dogs' teeth were instrumented and filled by the lateral condensation technique with gutta-percha points and Endomethasone or CRCS sealers. After post space preparation, the remaining filling material was protected or not with a plug of temporary Coltosol sealer and exposed to the oral environment for 90 days. Thereafter, the animals were sacrificed and the specimens were removed and prepared for histomorphological and histobacteriological analysis. The findings revealed 35% of microbial leakage in the groups without plugs and 15% of leakage in the groups with plugs. Statistical analysis showed that the use of a Coltosol plug improved significantly the histomorphological results regardless of the type of root canal sealer (p=0.05) and that CRCS and Endomethasone sealers showed similar results (p>0.05). O propósito deste trabalho foi avaliar a influência da infiltração coronária no reparo dos tecidos periapicais após obturação dos canais radiculares, preparo para pino e proteção ou não de um "plug" de cimento temporário. Quarenta canais de dentes de cães foram instrumentados e obturados pela técnica da condensação lateral ativa com cones de guta-percha e os cimentos Endomethasone e CRCS. Após preparo para pino os remanescentes do material obturador foram protegidos ou não com um "plug" do cimento temporário Coltosol e expostos ao meio oral por 90 dias. Decorrido este período, os animais foram mortos e os espécimes foram removidos e preparados para análises histomorfológica e histobacteriológica. Foi observado 35% de casos de infiltração bacteriana nos grupos sem "plug" e 15% nos grupos com "plug". Concluiu-se estatisticamente que o "plug" de Coltosol foi eficiente no controle da infiltração coronária de microorganismos (p=0,05), e que os cimentos CRCS e Endomethasone apresentaram resultados semelhantes (p>0,05).
- Published
- 2007
9. Reversal of radiocontrast medium toxicity in human renal proximal tubular cells by white grape juice extract
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Ashour Michael, Michele Navarra, Domenico Russo, Massimo Sabbatini, Fulvio Mattivi, Michele Andreucci, Antonio Pisani, Teresa Faga, Giovambattista De Sarro, Andreucci, Michele, Faga, Teresa, Pisani, Antonio, Sabbatini, Massimo, Russo, Domenico, Mattivi, Fulvio, De Sarro, Giovambattista, Navarra, Michele, and Michael, Ashour
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Kinase ,Cell Survival ,Signaling ,Renal cell ,Vitis spp ,Cell injury ,Contrast Media ,Diatrizoate ,FOXO1 ,Signalling ,Pharmacology ,Biology ,Toxicology ,Protective Agents ,Plant Extract ,Nephrotoxicity ,Cell Line ,Beverages ,Kidney Tubules, Proximal ,medicine ,Settore CHIM/08 - CHIMICA FARMACEUTICA ,Humans ,Vitis ,Viability assay ,Phosphorylation ,Beverage ,Protein kinase B ,Protective Agent ,Chinasi ,Forkhead Box Protein O1 ,Plant Extracts ,Forkhead Box Protein O3 ,Forkhead Transcription Factors ,Forkhead Transcription Factor ,General Medicine ,Viti ,Acute Kidney Injury ,Biochemistry ,Cellule renali ,Toxicity ,Danno cellulare ,Proto-Oncogene Proteins c-akt ,Human ,medicine.drug ,Signal Transduction - Abstract
Radiocontrast media (RCM)-induced nephrotoxicity (CIN) is a major clinical problem accounting for 12% of all hospital-acquired cases of acute kidney injury. The pathophysiology of CIN is not well understood, but direct toxic effects on renal cells have been postulated as contributing to CIN. We have investigated the effect of a white grape (Vitis vinifera) juice extract (WGJe) on human renal proximal tubular (HK-2) cells treated with the radiocontrast medium (RCM) sodium diatrizoate. WGJe caused an increase in phosphorylation of the prosurvival kinases Akt and ERK1/2 in HK-2 cells. Treatment of HK-2 cells with 75 mg I/ml sodium diatrizoate for 2.5 h and then further incubation (for 27.5 h) after removal of the RCM caused a drastic decrease in cell viability. However, pre-treatment with WGJe, prior to incubation with diatrizoate, dramatically improved cell viability. Analysis of key signaling molecules by Western blotting showed that diatrizoate caused a drastic decrease in phosphorylation of Akt (Ser473), FOXO1 (Thr24) and FOXO3a (Thr32) during the initial 2.5 h incubation period, and WGJe pre-treatment caused a reversal of these effects. Further analysis by Western blotting of samples from HK-2 cells cultured for longer periods of time (for up to 27.5 h after an initial 2.5 h exposure to diatrizoate with or without WGJe pre-treatment) showed that WGJe pre-treatment caused a negative effect on phosphorylation of p38, NF-jB (Ser276) and pERK1/2 whilst having a positive effect on the phosphorylation of Akt, FOXO1/FOXO3a and maintained levels of Pim-1 kinase. WGJe may alleviate RCM toxicity through modulation of signaling molecules that are known to be involved in cell death and cell survival and its possible beneficial effects should be further investigated.
- Published
- 2015
10. ALTERNATIVE METHODS TO PRESERVE PEPPER PASTE QUALITY: EFFECT OF TEMPERATURE AND PROTECTIVE AGENTS
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Ruhan Aşkin Uzel
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Chemistry ,Enthalpy,pepper paste,polyethylene terephthalate (PET),protective agent ,Final product ,Mühendislik ,food and beverages ,General Medicine ,Raw material ,medicine.disease_cause ,Engineering ,Starter ,Enthalpy ,lcsh:TA1-2040 ,Protective Agents ,Mold ,lcsh:Technology (General) ,Pepper ,medicine ,lcsh:T1-995 ,pepper paste ,Food science ,lcsh:Engineering (General). Civil engineering (General) ,Sugar ,polyethylene terephthalate (PET) ,Flavor ,protective agent - Abstract
Unlike conventional methods, red pepper paste was produced by various techniques using starter cultures. Products from paste process were stored at 37°C for 60 days. Salinity, acidity, color parameters, invert sugar, brown pigment formation values were determined together with microbiological, statistical and sensory evaluations at different filling temperatures with and without protective agents. Microbiological analysis showed that E. coli was not detected in raw material and in the final product at any production stage, however mold and yeast growth started from beginning of the second storage period. Increase in total sugar content of the final product via protective agent use caused a higher acidity, in other terms a lower pH value. Sweet red pepper paste treated with protective agents at higher temperature was found to be best with respect to panelists’ flavor and color quality scores. The study showed the necessity to use protective agents in red pepper paste production.
- Published
- 2017
11. The renoprotective effects of mannitol and udenafil in renal ischemia-reperfusion injury model
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Vural Kucukatay, Ali Ersin Zumrutbas, Nilay Şen Türk, Yusuf Özlülerden, Hülya Aybek, and Cihan Toktaş
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creatinine blood level ,medicine.medical_treatment ,pyrimidine derivative ,030232 urology & nephrology ,Urine ,Wistar rat ,Kidney ,lcsh:RC870-923 ,Nephrectomy ,neutrophil gelatinase-associated lipocalin protein, rat ,urea nitrogen blood level ,Udenafil ,chemistry.chemical_compound ,0302 clinical medicine ,Ngal protein ,Malondialdehyde ,creatinine clearance ,Medicine ,Mannitol ,rat ,animal ,ligation ,kidney function ,Basic and Translational Research ,Comet assay ,Ischemia reperfusion injury ,Acute-Phase Proteins/metabolism ,Animals ,Constriction ,Diuretics, Osmotic/*pharmacology ,Female ,Kidney/*blood supply ,Lipocalins/metabolism ,Malondialdehyde/metabolism ,Mannitol/*pharmacology ,Phosphodiesterase 5 Inhibitors/*pharmacology ,Protective Agents/pharmacology ,Proto-Oncogene Proteins/metabolism ,Pyrimidines/*pharmacology ,Rats, Wistar ,Reperfusion Injury/*prevention & control ,Sulfonamides/*pharmacology ,Blood urea nitrogen ,Sulfonamides ,acute phase protein ,malonaldehyde ,osmotic diuretic agent ,reperfusion injury ,Diuretics, Osmotic ,Lipocalins ,female ,Udenafil this ,oncoprotein ,Reperfusion Injury ,030220 oncology & carcinogenesis ,histopathology ,Original Article ,lipocalin ,medicine.drug ,medicine.medical_specialty ,Urology ,Protective Agents ,Article ,animal tissue ,lipid peroxidation assay ,03 medical and health sciences ,Lipocalin-2 ,vascularization ,comet assay ,Proto-Oncogene Proteins ,udenafil ,sulfonamide ,spectrophotometry ,controlled study ,protective agent ,Creatinine ,nonhuman ,business.industry ,animal model ,kidney ischemia ,Phosphodiesterase 5 Inhibitors ,lcsh:Diseases of the genitourinary system. Urology ,Pyrimidines ,chemistry ,DNA damage ,phosphodiesterase V inhibitor ,business ,experimental kidney disease ,metabolism ,neutrophil gelatinase associated lipocalin ,urine sampling ,Acute-Phase Proteins - Abstract
Purpose: The aim of this study was to investigate and compare the effects of udenafil and mannitol in an experimental renal ischemia-reperfusion (I/R) injury model., Materials and Methods: A total of 64 female Wister Albino rats were used. Right nephrectomy was performed in all groups. In the control group; I/R injury was not performed. In the I/R group; left renal pedicle was clamped for 45 minutes and then underwent 60 minutes and 24 hours of reperfusion. In the mannitol group; 1 mL 20% mannitol was given intravenously 15 minutes before clamping. In the udenafil group; 10-mg/kg udenafil was given orally 1 hour before clamping. Creatinine (Cr), blood urea nitrogen (BUN), Cr clearance, malondialdehyde, neutrophil gelatinase associated lipocalin (NGAL), histological examination and DNA damage (Comet Assay method) levels were compared in tissue, serum and urine samples., Results: Udenafil had a better protective effect than mannitol according to biochemical parameters (Cr, BUN, Cr clearance, and NGAL levels) and histopathological findings when compared with the I/R group. In the Comet sampling analysis no significant difference was detected., Conclusions: Udenafil has a better renoprotective effect than mannitol against I/R injury and this effect supports more functional improvements. Further clinical trials are needed to demonstrate those effects and clinical utility of udenafil for that purpose in humans.
- Published
- 2017
12. Beneficial effects montelukast, cysteinyl-leukotriene receptor antagonist, on renal damage after unilateral ureteral obstruction in rats
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Nurver Ozbay, Mustafa Cekmen, Emin Ozbek, Levent Ozcan, Adnan Somay, Emre Can Polat, Suleyman Sami Cakir, Alper Otunctemur, Murat Dursun, and Bayburt University
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Cyclopropanes ,Male ,Wistar rat ,Acetates ,lcsh:RC870-923 ,Kidney ,chemistry.chemical_compound ,Fibrosis ,Malondialdehyde ,Urea ,animal ,Renal Insufficiency ,Leukotriene ,leukotriene ,malonaldehyde ,Glutathione ,medicine.anatomical_structure ,Treatment Outcome ,Creatinine ,montelukast [Supplementary Concept] ,Montelukast Sodium ,montelukast ,Quinolines ,acetic acid derivative ,Original Article ,Cysteinyl-leukotriene[supplementary concept] ,quinoline derivative ,medicine.drug ,Ureteral Obstruction ,medicine.medical_specialty ,Leukotrienes ,leukotriene receptor blocking agent ,Urology ,complication ,cysteinyl-leukotriene [Supplementary Concept] ,Sulfides ,Nitric Oxide ,Protective Agents ,blood ,Internal medicine ,medicine ,Animals ,Cysteine ,Rats, Wistar ,reproducibility ,Montelukast ,antagonists and inhibitors ,protective agent ,business.industry ,urogenital system ,Antagonist ,Reproducibility of Results ,medicine.disease ,lcsh:Diseases of the genitourinary system. Urology ,Oxidative Stress ,Endocrinology ,chemistry ,ureter obstruction ,drug effects ,Leukotriene Antagonists ,pathology ,cysteinyl-leukotriene ,Lipid Peroxidation ,business - Abstract
WOS: 000354634500014 PubMed ID: 26005969 Introduction: Ureteral obstruction is a common pathology and caused kidney fibrosis and dysfunction at late period. In this present, we investigated the antifibrotic and antiinflammatory effects of montelukast which is cysteinyl leukotriene receptor antagonist, on kidney damage after unilateral ureteral obstruction(UUO) in rats. Materials and Methods: 32 rats divided four groups. Group 1 was control, group 2 was sham, group 3 was rats with UUO and group 4 was rats with UUO which were given montelukast sodium (oral 10 mg/kg/day). After 14 days, rats were killed and their kidneys were taken and blood analysis was performed. Tubular necrosis, mononuclear cell infiltration and interstitial fibrosis scoring were determined histopathologically in a part of kidneys; nitric oxide(NO), malondialdehyde(MDA) and reduced glutathione(GSH) levels were determined in the other part of kidneys. Urea-creatinine levels were investigated at blood analysis. Statistical analyses were made by the Chi-square test and one-way analysis of variance (ANOVA). Results: There was no difference significantly for urea-creatinine levels between groups. Pathologically, there was serious tubular necrosis and fibrosis in group 3 and there was significantly decreasing for tubular necrosis and fibrosis in group 4(p
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- 2014
13. [60]Fullerene derivative modulates adenosine and metabotropic glutamate receptors gene expression: a possible protective effect against hypoxia
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Mairena Martín, Davide Giust, Tatiana Da Ros, José Luis Albasanz, Giust, Davide, Da Ros, Tatiana, Martín, Mairena, and Albasanz, José L.
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Adenosine ,Cell Survival ,Biomedical Engineering ,Pharmaceutical Science ,Adenosine receptors ,Fullerenes ,Hypoxia ,Metabotropic glutamate receptors ,Neuroprotection ,Cell Hypoxia ,Cell Line, Tumor ,Gene Expression Regulation, Neoplastic ,Humans ,Protective Agents ,Receptors, Metabotropic Glutamate ,Tetrazolium Salts ,Thiazoles ,Bioengineering ,Medicine (miscellaneous) ,Molecular Medicine ,Applied Microbiology and Biotechnology ,3003 ,Biology ,Pharmacology ,Cell Line ,Fullerene ,Receptors ,Metabotropic Glutamate ,Protective Agent ,Neoplastic ,Tumor ,Metabotropic glutamate receptor 5 ,Metabotropic glutamate receptor ,Metabotropic glutamate receptor 4 ,Research ,Metabotropic glutamate receptor 7 ,Adenosine receptor ,Metabotropic glutamate receptor 6 ,Gene Expression Regulation ,Metabotropic glutamate receptor 1 ,Metabotropic glutamate receptor 3 ,Thiazole ,Metabotropic glutamate receptor 2 ,Tetrazolium Salt ,Human - Abstract
Background: Glutamate, the main excitatory neurotransmitter, is involved in learning and memory processes but at higher concentration results excitotoxic causing degeneration and neuronal death. Adenosine is a nucleoside that exhibit neuroprotective effects by modulating of glutamate release. Hypoxic and related oxidative conditions, in which adenosine and metabotropic glutamate receptors are involved, have been demonstrated to contribute to neurodegenerative processes occurring in certain human pathologies. Results: Human neuroblastoma cells (SH-SY5Y) were used to evaluate the long time (24, 48 and 72 hours) effects of a [60]fullerene hydrosoluble derivative (t3ss) as potential inhibitor of hypoxic insult. Low oxygen concentration (5% O2) caused cell death, which was avoided by t3ss exposure in a concentration dependent manner. In addition, gene expression analysis by real time PCR of adenosine A1 ,A 2A and A2B and metabotropic glutamate 1 and 5 receptors revealed that t3ss significantly increased A1 and mGlu1 expression in hypoxic conditions. Moreover, t3ss prevented the hypoxia-induced increase in A2A mRNA expression. Conclusions: As t3ss causes overexpression of adenosine A1 and metabotropic glutamate receptors which have been shown to be neuroprotective, our results point to a radical scavenger protective effect of t3ss through the enhancement of these neuroprotective receptors expression. Therefore, the utility of these nanoparticles as therapeutic target to avoid degeneration and cell death of neurodegenerative diseases is suggested.
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- 2014
14. Protective Effect Of Esterified Glucomannan On Aflatoxin-Induced Changes In Testicular Function, Sperm Quality, And Seminal Plasma Biochemistry In Rams
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Emrah Sur, Kenan Çoyan, Nurcan Dönmez, Hasan Hüseyin Dönmez, Mehmet Bozkurt Ataman, and Mustafa Numan Bucak
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animal food ,Male ,Aflatoxin ,Glucomannan ,Mannans ,chemistry.chemical_compound ,Food Animals ,Aflatoxins ,reproductive parameters ,Malondialdehyde ,animal ,Sperm quality ,glutathione ,Small Animals ,Sperm motility ,(1-6)-alpha-glucomannan ,drug effect ,malonaldehyde ,article ,mannan ,ultrastructure ,Testicular function ,Sperm Motility ,acrosome ,medicine.medical_specialty ,sheep ,Hyaluronoglucosaminidase ,testis ,Protective Agents ,sperm ,Andrology ,Semen ,Internal medicine ,medicine ,Animals ,Acrosome ,protective agent ,Equine ,(1 6) alpha glucomannan ,spermatozoon motility ,Animal Feed ,Abnormal spermatozoa ,RAM ,Endocrinology ,chemistry ,physiology ,Animal Science and Zoology ,metabolism - Abstract
The aim of this study was to determine the effect of aflatoxin (AF) on spermatologic, biochemical, and testis parameters in rams, and the protective efficiency of esterified glucomannan (EG) co-administered with AF. Thirty-two Merino rams (12-14 months old) were used. The experimental design consisted of four dietary treatments. The control group was fed commercial feed. The AF group was fed with commercial feed plus 250 mu g/d of total AF. The EG group received commercial feed plus 2 g/d of EG. The AF + EG group was given commercial feed plus 250 mu g/d of total AF and 2 g/d of EG. There were treatment, time, and treatment-by-time interaction effects on sperm motility, abnormal spermatozoa, damaged acrosome, and dead spermatozoa (P < 0.01). The percentage of motile sperm was lower and the percentages of abnormal sperm, sperm with damaged acrosomes, and dead sperm were greater in the AF group than in the control, AF+EG, and EG groups, as from week 3 until the end of week 12 (P < 0.05). As from week 3, hyaluronidase activity in the seminal plasma increased significantly in the AF group, compared with the control. The co-administration of AF+EG was found to be effective in preventing the increase in hyaluronidase activity. As week 4, malondialdehyde (MDA) levels were significantly higher in the AF group compared with the control. The combined administration of AF+EG was found to be effective in lowering the MDA levels, increased by AF, to the levels measured in the control (P < 0.05). Although glutathione (GSH) levels were determined to have significantly decreased in the AF group in comparison to the control, it was observed that, in the group co-administered with AF and EG, particularly after week 7, the GSH levels, which had decreased owing to AF, were largely ameliorated (P < 0.05). In conclusion, AF adversely affected spermatologic, biochemical, and testis parameters, and the combined administration of EG with AF reversibly eliminated these adverse effects in rams. Crown Copyright (C) 2014 Published by Elsevier Inc. All rights reserved.
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- 2014
15. Growth Hormone and Hexarelin Prevent Endothelial Vasodilator Dysfunction in Aortic Rings of the Hypophysectomized Rat
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Antonio Torsello, M Bernareggi, Boghen M, Locatelli, Nilsson M, Eugenio E. Müller, Ferruccio Berti, Giuseppe Rossoni, Schweiger F, De Gennaro Colonna, Rossoni, G, Locatelli, V, De Gennaro Colonna, V, Torsello, A, Schweiger, F, Boghen, M, Nilsson, M, Bernareggi, M, Müller, E, and Berti, F
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Male ,medicine.medical_specialty ,Hypophysectomy ,Endothelium ,medicine.medical_treatment ,Vasodilation ,6-Ketoprostaglandin F1 alpha ,Biology ,Protective Agents ,Rats, Sprague-Dawley ,Internal medicine ,medicine.artery ,Homeostasi ,medicine ,Animals ,Homeostasis ,Humans ,Enzyme Inhibitor ,Enzyme Inhibitors ,BIO/14 - FARMACOLOGIA ,Aorta ,Protective Agent ,Pharmacology ,omega-N-Methylarginine ,Endothelin-1 ,Animal ,Human Growth Hormone ,Body Weight ,Biological activity ,Endothelin 1 ,Acetylcholine ,Rats ,Endocrinology ,medicine.anatomical_structure ,Circulatory system ,Rat ,Oligopeptide ,Endothelium, Vascular ,Cardiology and Cardiovascular Medicine ,Oligopeptides ,Human ,Blood vessel - Abstract
The endothelial vasodilation mechanism(s) has been investigated in aortic rings of hypophysectomized male rats as well as hypophysectomized rats treated for 7 days with growth hormone (GH, 400 microg/kg, s.c.) or hexarelin (80 microg/kg, s.c.). Tissue preparations from intact animals were taken as controls. The results obtained indicate that the release of 6-keto-prostaglandin F1alpha (6-keto-PGF1alpha) from aortic rings of hypophysectomized rats was markedly reduced (51%; p
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- 1999
16. Artichoke for biochemistry, histology, and gene expression in obstructive jaundice
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Metabolic Syndrome ,Leaf Extract Supplementation ,Oxidative Stress ,Damage ,Liver ,Obstructive jaundice ,Toxicity ,Cynara scolymus ,Apoptosis ,Disease ,Protective agent ,Antioxidant ,Tcf7l2-Rs7903146 - Abstract
OBJECTIVE: This study aimed to evaluate the hepatoprotective effect of artichoke leaf extract (Cynara scolymus) in experimental obstructive jaundice. METHODS: Rats were separated into three groups, namely, sham, control, and artichoke leaf extract. Ischemia was created for 60 min, and then liver tissue and blood samples were taken at the 90th minute of reperfusion. Artichoke leaf extract was given at a 300 mg/kg dose 2 h before the operation. Antioxidant enzyme activities and biochemical parameters were examined from the tissue and serum. Histopathological findings of the liver were scored semiquantitatively. RESULTS: Antioxidant enzyme activities in the artichoke leaf extract group were statistically significantly higher than that in the other two groups. Biochemical parameters, which show hepatocellular damage, were found to be similar in both sham and artichoke leaf extract groups. Although the values in the sham group were higher than the artichoke group in terms of protein and gene expressions, no statistically significant difference was found between these two groups. Regarding the hepatocellular effects of obstructive jaundice, the artichoke leaf extract group showed lower scores than the control group in all histopathological scores. CONCLUSION: The results of this study showed that artichoke leaf extract had a hepatoprotective effect that was associated with the antioxidant and anti-inflammatory effects of artichoke leaf extract.
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