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Alkynones represent one class of interesting building block which are frequently founded in a variety of biologically active compounds.[1] They have been emerged as key intermediates in the synthesis of various natural products and heterocycles.[2] Therefore, a considerable interest has been attracted and numerous strategies have been explored for the synthesis of alkynones. The traditional methods mainly rely on the reactions of acid chlorides with terminal alkynes.>[3–4] However, some drawbacks remain existing, including high reaction temperature, unstable acid chlorides, and poor functional group tolerance. [ABSTRACT FROM AUTHOR]

Stochastic averaging, as an effective technique for dimension reduction, is of great significance in stochastic dynamics and control. However, its practical applications in industrial and engineering fields are severely hindered by its dependence on governing equations and the complexity of mathematical operations. Herein, a data-driven method, named data-driven stochastic averaging, is developed to automatically discover the low-dimensional stochastic differential equations using only the random state data captured from the original high-dimensional dynamical systems. This method includes two successive steps, that is, extracting all slowly varying processes hidden in fast-varying state data and identifying drift and diffusion coefficients by their mathematical definitions. It automates dimension reduction and is especially suitable for cases with unavailable governing equations and excitation data. Its application, efficacy, and comparison with theory-based stochastic averaging are illustrated through several examples, numerical or experimental, with pure Gaussian white noise excitation or combined excitations. [ABSTRACT FROM AUTHOR]

Soil wind erosion is one of the critical drivers of land degradation in the black soil region of northeastern China. In this study, 137Cs background value and soil wind erosion rate was studied by using 137Cs nuclide tracing technique in the black soil region of northeastern China, and wind erosion characteristics such as background value and soil erosion rate over the years were clarified, which has guiding significance for soil and water conservation and agricultural sustainable development in the black soil region of northeast China. The mean area activity of the Hilltop area was 1541 Bq·m-2, which was lower than the background value of 1879.7 Bq·m-2 in the study area. At the top of the hill, soil erosion occurred to some extent. The 137Cs profile has distributional characteristics that can be divided into three categories. Most of the 137Cs area activity in Hilltop area is evenly distributed in 0-15 cm, and the 137Cs area activity below 15 cm decreased gradually until it disappeared. The spatial difference of wind erosion rate is significant in the study area, mainly between 7.17-10.13 t·hm-2a-1. The average wind erosion rate of hilltops with different tillage quality depths has little difference. The average wind erosion rate is 8.60 t·hm-2a-1, which belongs to mild erosion. However, its erosion rate is higher than the tolerable soil loss of 2 t·hm-2a-1 in the black soil region of northeast China. As a result, soil erosion control in the black soil region of northeast China still requires attention. [ABSTRACT FROM AUTHOR]

High borated steels (HBSs) exhibit poor mechanical properties because brittle and coarse borides served as crack source during deformation, causing a premature failure. Here we proposed a novel strategy of tailoring strain hardening behaviour of metal matrix to enhance performance. Metastable austenite was introduced as matrix, where various deformation mechanism was obtained by manipulating deform temperature to change stacking fault energy (SFE). Unexpectedly, extraordinary strength-ductility synergy was achieved in HBSs with a moderate SFE at 100 °C. During hetero-deformation, hard borides caused serious stress gradients in austenite, triggering discontinuous transformation-induced plasticity effect. The cavities originating from fractured borides and interface debonding were arrested effectively by transformed martensite, which highly retarded the coalescence to form large cracks, allowing residual strain hardening to extend much longer to induce additional ductility. This work provides a new pathway to enhance strength-ductility synergy of materials containing many coarse and brittle secondary phases. [Display omitted] [ABSTRACT FROM AUTHOR]

C8H6N8O8, orthorhombic, P212121 (no. 19), a = 6.7563(9) Å, b = 7.2512(12) Å, c = 25.884(3) Å, V = 1268.1(3) Å3, β = 90°, Z = 4, Rgt(F) = 0.0473, wRref(F2) = 0.1170, T = 170 K. [ABSTRACT FROM AUTHOR]

Drought and flooding are the two most important environmental factors limiting maize (Zea mays L.) production globally. This study aimed to investigate the physiological mechanisms and accurate evaluation indicators and methods of maize germplasm involved in drought and flooding stresses. The twice replicated pot experiments with 60 varieties, combined with the field validation experiment with 3 varieties, were conducted under well-watered, drought, and flooding conditions. Most varieties exhibited stronger tolerance to drought than flooding due to higher antioxidant enzyme activities, osmotic adjustment substances, and lower reactive oxygen species. In contrast, flooding stress resulted in higher levels of reactive oxygen species (particularly O2 -), ascorbate peroxidase, catalase, peroxidase, and soluble sugars but lower levels of superoxide dismutase, proline, and soluble protein compared with well-watered conditions. Superoxide dismutase, peroxidase, catalase, ascorbate peroxidase, proline, soluble sugars, and protein contents, in addition to plant height, leaf area/plant, and stem diameter, were accurate and representative indicators for evaluating maize tolerance to drought and flooding stresses and could determine a relatively high mean forecast accuracy of 100.0% for the comprehensive evaluation value. A total of 4 principal components were extracted, in which different principal components played a vital role in resisting different water stresses. Finally, the accuracy of the 3 varieties screened by multivariate analysis was verified in the field. This study provides insights into the different physiological mechanisms and accurate evaluation methods of maize germplasm involved in drought and flooding stresses, which could be valuable for further research and breeding. [ABSTRACT FROM AUTHOR]

Canonical equations play a pivotal role in various sub-fields of physics and mathematics. However, for complex systems and systems without first principles, deriving canonical equations analytically is quite laborious or might even be impossible. This work is devoted to automatedly distilling the canonical equations solely from random state data. The random state data are collected from stochastically excited, dissipative dynamical systems either experimentally or numerically, while other information, such as the system characterization itself and the excitations, is not needed. The identification procedure comes down to a nested optimization problem, and the explicit expressions of the momentum (density) functions and energy (density) functions are identified simultaneously. Three representative examples are investigated to illustrate its high accuracy of identification, the small requirement for data amount, and high robustness to excitations and dissipation. The identification procedure serves as a filter, filtering out nonconservative information while retaining conservative information, which is especially suitable for systems with unobtainable excitations. [ABSTRACT FROM AUTHOR]

Aiming at SDN-based storage environment, the integrity and availability of storage service are threatened by many attacks from data plane, such as packet tampering and dropping. While existing packet forwarding verification methods to deal with the sophisticated attacks are extremely limited due to the assumption that all or part of the switches are trusted. In this paper, for the untrusted data plane, a transparent gateway-based packet forwarding verification mechanism, named GwPFV, was designed to mitigate and trace back the attacks, specially utilizing the characteristics of TCP protocol to realize a packet obfuscation method to proactively defend attacks, a fast event-counting method to find attacks, and a path switching method involved with SDN controller to locate malicious switches. Finally, both the effectiveness and security of GwPFV are analyzed in depth. The experiments performed in an SDN-based prototype storage system reveal that it can achieve the security goal with relative low performance overhead. [ABSTRACT FROM AUTHOR]

In this paper, we prove the Kastler-Kalau-Walze type theorems for twisted Dirac operators on 5-dimensional manifolds with boundary. [ABSTRACT FROM AUTHOR]

Objective: Adipocyte differentiation is regulated by a variety of functional genes and noncoding RNAs. However, the role of miRNAs in lipid deposition of goat white adipose tissue is still unclear. Therefore, this study revealed the miRNA expression profile in goat subcutaneous adipocytes by sRNA-seq. Methods: The miRNA expressed in goat subcutaneous preadipocytes and the mature adipocytes were sequenced by sRNA-seq. The differentially expressed miRNAs (DEm) were screened and gene ontology (GO) and Kyoto encyclopedia for genes and genomes (KEGG) analyses were performed. Gain-of-function and loss-of-function combined with oil red O staining, Bodipy staining, and quantitative reverse-transcription polymerase chain reaction (qPCR) were utilized to determine the effect of miR-133a-3p on adipocyte differentiation. Results: A total of 218 DEm were screened out. The target genes of these DEm were significantly enriched in GO items such as biological regulation and in KEGG terms such as FAK signaling pathway and MAPK signaling pathway. qPCR verified that the expression trend of miRNA was consistent with miRNA-seq. The gain-of-function or loss-of-function of miR-133a-3p showed that it promoted or inhibited the accumulation of lipid droplets, and CCAAT enhancer binding protein α (C/EBPα) and C/EBPβ were extremely significantly up-regulated or down-regulated respectively (p<0.01), the loss-of-function also led to a significant down-regulation of peroxisome proliferator activated receptor gamma (PPARγ) (p<0.01). Conclusion: This study successfully identified miRNAs expression patterns in goat subcutaneous adipocytes, and functional identification indicates that miR-133a-3p is a positive regulator of the differentiation process of goat subcutaneous adipocytes. Our results lay the foundation for the molecular mechanism of lipid deposition in meat-source goats from the perspective of miRNA. [ABSTRACT FROM AUTHOR]

Men or mice with homozygous serine/threonine kinase 33 (STK33) mutations are sterile owing to defective sperm morphology and motility. To chemically evaluate STK33 for male contraception with STK33-specific inhibitors, we screened our multibillion-compound collection of DNA-encoded chemical libraries, uncovered potent STK33-specific inhibitors, determined the STK33 kinase domain structure bound with a truncated hit CDD-2211, and generated an optimized hit CDD-2807 that demonstrates nanomolar cellular potency (half-maximal inhibitory concentration = 9.2 nanomolar) and favorable metabolic stability. In mice, CDD-2807 exhibited no toxicity, efficiently crossed the blood-testis barrier, did not accumulate in brain, and induced a reversible contraceptive effect that phenocopied genetic STK33 perturbations without altering testis size. Thus, STK33 is a chemically validated, nonhormonal contraceptive target, and CDD-2807 is an effective tool compound. [ABSTRACT FROM AUTHOR]

The endosomal sorting complexes required for transport (ESCRTs) are responsible for membrane remodeling in many cellular processes, such as multivesicular body biogenesis, viral budding, and cytokinetic abscission. ESCRT-III, the most abundant ESCRT subunit, assembles into flat spirals as the primed state, essential to initiate membrane invagination. However, the three-dimensional architecture of ESCRT-III flat spirals remained vague for decades due to highly curved filaments with a small diameter and a single preferred orientation on the membrane. Here, we unveiled that yeast Snf7, a component of ESCRT-III, forms flat spirals on the lipid monolayers using cryogenic electron microscopy. We developed a geometry-constrained Euler angle--assigned reconstruction strategy and obtained moderate-resolution structures of Snf7 flat spirals with varying curvatures. Our analyses showed that Snf7 subunits recline on the membrane with N-terminal motifs α0 as anchors, adopt an open state with fused α2/3 helices, and bend α2/3 gradually from the outer to inner parts of flat spirals. In all, we provide the orientation and conformations of ESCRT-III flat spirals on the membrane and unveil the underlying assembly mechanism, which will serve as the initial step in understanding how ESCRTs drive membrane abscission. [ABSTRACT FROM AUTHOR]

Plants often adapt to adverse or stress conditions via differential growth. The trans-Golgi network (TGN) has been implicated in stress responses, but it is not clear in what capacity it mediates adaptive growth decisions. In this study, we assess the role of the TGN in stress responses by exploring the previously identified interactome of the Transport Protein Particle II (TRAPPII) complex required for TGN structure and function. We identified physical and genetic interactions between AtTRAPPII and shaggy-like kinases (GSK3/AtSKs) and provided in vitro and in vivo evidence that the TRAPPII phosphostatus mediates adaptive responses to abiotic cues. AtSKs are multifunctional kinases that integrate a broad range of signals. Similarly, the AtTRAPPII interactome is vast and considerably enriched in signaling components. An AtSK-TRAPPII interaction would integrate all levels of cellular organization and instruct the TGN, a central and highly discriminate cellular hub, as to how to mobilize and allocate resources to optimize growth and survival under limiting or adverse conditions. [ABSTRACT FROM AUTHOR]

A miniaturized, low-cost, 4-channel fiber Bragg grating (FBG) interrogation system for real-time remote monitoring is presented in this paper. A superluminescent light emitting diode (SLED) as the broadband light source combined with an interrogation module and an optical switch is applied in the system. The moving average method is employed to remove noise effects, and then Gaussian fitting method is used to realize the interrogation of FBG central wavelength with high precision. On software design, the WebSocket network protocol is introduced to establish communication between data processing core and webpages to achieve cross-platform remote access through the browser without user's software installation. The proposed interrogation system not only displays the reflected spectrum and demodulated central wavelength in real time on the webpage, but also realizes the data storage and channel switch remotely. The interrogator for remote monitoring of FBG sensors proposed in this paper has been proved experimentally with the interrogation resolution 0.1 pm, the precision of 5 pm for the FBG central wavelength, interrogation stability up to ± 1.1 pm, and interrogation frequency of 4 kHz. [ABSTRACT FROM AUTHOR]

Epidermal growth factor receptor (EGFR) is frequently overexpressed in multiple malignancies. Icotinib (IH), a new EGFR tyrosine kinase inhibitor, enhances radiosensitivity in various types of cancer, but its effect on nasopharyngeal carcinoma (NPC) remains unclear. Total 115 NPC tissue sections and 30 nasopharyngitis tissue sections were enrolled. The correlation of EGFR expression and clinicopathologic features of NPC was analyzed. Survival analysis was calculated by using univariate and multivariate regression analysis. A radioresistant NPC cell line, CNE-2R, was established with a gradient irradiation schedule. Cell viability, colony formation and EGFR expression of CNE-2/2R cells were examined. Significant higher expression of EGFR was observed in NPC tissues than chronic nasopharyngitis lesions. EGFR expression was significantly correlated with both tumor stage (P < 0.001) and tumor-node-metastasis stage of NPC (P = 0.006). EGFR expression was an independent prognostic factor of disease-free survival (P = 0.047) and the overall survival of NPC (P = 0.016). Cell viability was higher in CNE-2R than CNE-2 on days 1, 2, 4, and 6 after radiation of 4 Gy. The colony number of CNE-2R was significantly higher than that of CNE-2 (P < 0.05), while IH enhanced the radiosensitizing effect of CNE-2R with lower survival fraction (P < 0.05). EGFR mRNA and protein expression levels were significantly higher in CNE-2R cells compared to CNE-2 cells, but significantly decreased after IH treatment (all P < 0.05). In conclusion, high EGFR expression is a poor prognostic factor for NPC patients. IH enhances the radiosensitivity of CNE-2R cells and reduce EGFR expression. [ABSTRACT FROM AUTHOR]

Background: To systematically analyze the influence of genetic polymorphisms of matrix metalloproteinase 9 (MMP9) on susceptibility to chronic obstructive pulmonary disease (COPD). Methods: Relevant literatures reporting MMP9 and susceptibility to COPD in PubMed, Web of Science, VIF, Wanfang and CNKI databases were searched using the key words »matrix metalloproteinases 9/MMP9, COPD/chronic obstructive pulmonary disease«. Data of eligible literatures were extracted and analyzed for the odds ratio (OR) and corresponding 95% CI. Results: A total of 16 independent studies reporting MMP9-1562C/T and COPD patients were enrolled and analyzed. None of the genetic models revealed the relationship between MMP9-1562C/T and susceptibility to COPD. Subgroup analyses identified lower risk of COPD in Chinese population carrying the TT genotype for theMMP-9 rs3918242 relative to those carrying CT and CC genotypes (P=0.03, OR=0.67, 95% CI=0.46-0.97). Conclusions: Chinese population carrying the TT genotype for the MMP-9 rs3918242 present lower susceptibility to COPD relative to those carrying CT and CC genotypes. [ABSTRACT FROM AUTHOR]

Objective: Herein, a feasible strategy based on a femtosecond laser (fs-laser) was provided to decelerate dentin wear, and the effect of wear resistance improvement and its potential mechanism were explored. Background: Tooth wear is a common phenomenon that exists throughout life. While once dentin is exposed, it wears away very quickly. Decelerating tooth wear, especially dentin wear, is an important issue, but there is no ideal treatment. Materials and methods: Sixteen third molar dentin samples were randomly divided into the amalgam (N1 = 8) and polymer infiltrated ceramic network (PICN) (N2 = 8) groups. One half of the sample was used as the experimental object and the other for the blank self-control. Array microcavities were fabricated on the experimental parts of all the samples with an fs-laser and then filled with the corresponding materials. The experimental and control parts of all the samples were subjected to 3600 cycles of sliding wear tests with titanium balls. The coefficients of friction (COFs) of every friction pair and the temperature rise were recorded in real time. The wear volume and depth were measured by a laser confocal microscopy. Statistical differences of wear volume and depth between the experimental and self-control parts were calculated by paired t-test. The wear mode was observed with scanning electron microscopy (SEM). Results: The COFs in the two experimental groups were significantly decreased. The maximum temperature rise on the dentin surface was 2.0C. The wear resistance in the amalgam and PICN groups was 4.48 and 3.53 times higher than blank dentin, respectively ( p < 0.001). The SEM images showed fewer plough grooves and cracks in dentin after microtexturing. Conclusions: This method could significantly improve tooth wear resistance by reducing the COFs between the friction pairs and reducing the dentin’s ploughing effect and provide new treatment ideas for slowing the loss of severely worn dentin. [ABSTRACT FROM AUTHOR]

Peste des petits ruminants is an acute and highly contagious disease caused by the Peste des petits ruminants virus (PPRV). Host proteins play a crucial role in viral replication. However, the effect of fusion (F) protein-interacting partners on PPRV infection is poorly understood. In this study, we found that the expression of goat plasminogen activator urokinase (PLAU) gradually decreased in a time- and dosedependent manner in PPRV-infected goat alveolar macrophages (GAMs). Goat PLAU was subsequently identified using co-immunoprecipitation and confocal microscopy as an F protein binding partner. The overexpression of goat PLAU inhibited PPRV growth and replication, whereas silencing goat PLAU promoted viral growth and replication. Additionally, we confirmed that goat PLAU interacted with a virus-induced signaling adapter (VISA) to antagonize F-mediated VISA degradation, increasing the production of type I interferon. We also found that goat PLAU reduced the inhibition of PPRV replication in VISA-knockdown GAMs. Our results show that the host protein PLAU inhibits the growth and replication of PPRV by VISA-triggering RIG-I-like receptors and provides insight into the host protein that antagonizes PPRV immunosuppression. IMPORTANCE The role of host proteins that interact with Peste des petits ruminants virus (PPRV) fusion (F) protein in PPRV replication is poorly understood. This study confirmed that goat plasminogen activator urokinase (PLAU) interacts with the PPRV F protein. We further discovered that goat PLAU inhibited PPRV replication by enhancing virus-induced signaling adapter (VISA) expression and reducing the ability of the F protein to degrade VISA. These findings offer insights into host resistance to viral invasion and suggest new strategies and directions for developing PPR vaccines. [ABSTRACT FROM AUTHOR]

Based on the laser flash method, the selection of experimental parameters on the accuracy of measurement results for measuring thermal diffusivity is investigated in this paper. High purity graphite is employed as the experimental material. Three experimental parameters are taken into consideration, including specimen thickness, laser pulse power, and laser pulse width. Firstly, the principle of the laser flash method is introduced. Then, a numerical simulation model is established and independence tests are performed. In order to investigate the impact of different experimental parameters to the precision of the measurement results using the laser flash method, six thicknesses of the specimens, five laser pulse powers, and five laser pulse widths are selected for numerical simulation and LFA 427 measurement experiments. Finally, an orthogonal design method with three-factor and three-level is constructed to investigate the influence degree of these three factors on the measurement results. It is found that the laser pulse width has the most significant influence, while the laser pulse power has the least impact. For high purity graphite specimens, it is required to choose a thicker specimen, a lower laser pulse power, and a smaller laser pulse width to ensure better measurement accuracy. [ABSTRACT FROM AUTHOR]

Background: Sperm quality plays an important role in the animal industry. The study of the sperm quality of Congjiang Xiang pigs is limited and the Rh123/PI double staining has not been used to detect the sperm quality of boars. Thus, the study aimed to evaluate the sperm quality of the Congjiang Xiang pigs and the feasibility of the flow cytometry (FCM) method in assessing sperm quality in pigs. Methods: Semen samples were evaluated for routine indicators of semen quality and using FCM with single staining and double staining to analyze the integrity of membrane and acrosome, the mitochondrial function. The sperm quality of the Congjiang Xiang pigs was compared with the Large White pigs. Result: The semen of the Congjiang Xiang pigs had a lower sperm concentration than that of the Large White pigs (P<0.01). There were no significant differences in sperm vitality, sperm motility and abnormality between the two breeds (P>0.05). Single sperm staining revealed a lower sperm acrosomal integrity in the semen of the Congjiang Xiang pigs than that of the Large White pigs (P<0.01). No significant difference between the two breeds in membrane integrity and mitochondrial function was observed (P>0.05). Rh123/PI double staining demonstrated that the rate of live sperm with a normal mitochondrial function of the Congjiang Xiang pigs was significantly higher than that of the Large White pigs (P<0.05). The rate of necrotic sperms in the semen of the Congjiang Xiang pigs was significantly lower than that in the semen of the Large White pigs (P<0.05). Correlation analysis revealed a positive association between the rates of live sperm in the semen of the congjiang xiang pigs and their sperm motility (P=0.051). Our results suggest that Rh123/PI double staining with flow cytometry can provide more reliable information to assess the boar sperm quality. [ABSTRACT FROM AUTHOR]

Organic electrodes mainly consisting of C, O, H, and N are promising candidates for advanced batteries. However, the sluggish ionic and electronic conductivity limit the full play of their high theoretical capacities. Here, we integrate the idea of metal-support interaction in single-atom catalysts with π--d hybridization into the design of organic electrode materials for the applications of lithium (LIBs) and potassium-ion batteries (PIBs). Several types of transition metal single atoms (e.g., Co, Ni, Fe) with π--d hybridization are incorporated into the semiconducting covalent organic framework (COF) composite. Single atoms favorably modify the energy band structure and improve the electronic conductivity of COF. More importantly, the electronic interaction between single atoms and COF adjusts the binding affinity and modifies ion traffic between Li/K ions and the active organic units of COFs as evidenced by extensive in situ and ex situ characterizations and theoretical calculations. The corresponding LIB achieves a high reversible capacity of 1,023.0 mA h g-1 after 100 cycles at 100 mA g-1 and 501.1 mA h g-1 after 500 cycles at 1,000 mA g-1. The corresponding PIB delivers a high reversible capacity of 449.0 mA h g-1 at 100 mA g-1 after 150 cycles and stably cycled over 500 cycles at 1,000 mA g-1. This work provides a promising route to engineering organic electrodes. [ABSTRACT FROM AUTHOR]

Nitrogen (N) is an essential nutrient for plant growth, and most plants absorb it as nitrate. AtNRG2 has been reported to play an important role in nitrate regulation. In this study, we investigated the functions of AtNRG2 family members of Arabidopsis thaliana and maize in nitrate signalling and metabolism. Our results showed that both AtNRG2.10 and AtNRG2.15 regulated nitrate signalling and metabolism. Overexpression of AtNRG2.11 (AtNRG2) could promote plant growth and improve nitrogen use efficiency (NUE). In addition, the maize genome harbors 23 ZmNRG2 members. We detected the expression of these genes treated with nitrate and the expression of four genes was strongly induced with ZmNRG2.7 having the highest levels. Overexpression of ZmNRG2.7 in the atnrg2 mutant could restore the defects of atnrg2, suggesting that ZmNRG2.7 is involved in nitrate signalling and metabolism. Moreover, the overexpression lines of ZmNRG2.7 showed increased biomass and NUE. These findings demonstrate that at least a part of NRG2 family genes in Arabidopsis and maize regulate nitrate signalling and provide a molecular basis for improving the NUE of crops. [ABSTRACT FROM AUTHOR]

Data-driven equation identification for dynamical systems has achieved great progress, which for static systems, however, has not kept pace. Unlike dynamical systems, static systems are time invariant, so we cannot capture discrete data along the time stream, which requires identifying governing equations only from scarce data. This work is devoted to this topic, building a data-driven method for extracting the differential-variational equations that govern static behaviors only from scarce, noisy data of responses, loads, as well as the values of system attributes if available. Compared to the differential framework typically adopted in equation identification, the differential-variational framework, due to its spatial integration and variation arbitrariness, brings some advantages, such as high robustness to data noise and low requirements on data amounts. The application, efficacy, and all the aforementioned advantages of this method are demonstrated by four numerical examples, including three continuous systems and one discrete system. [ABSTRACT FROM AUTHOR]

Diffusible signal factor (DSF) family signals represent a unique group of quorum sensing (QS) chemicals that modulate a wide range of behaviors for bacteria to adapt to different environments. However, whether DSF-mediated QS signaling acts as a public language to regulate the behavior of biocontrol and pathogenic bacteria remains unknown. In this study, we present groundbreaking evidence demonstrating that RpfFXc1 or RpfFOH11 could be a conserved DSF-family signal synthase in Xanthomonas campestris or Lysobacter enzymogenes. Interestingly, we found that both RpfFOH11 and RpfFXc1 have the ability to synthesize DSF and BDSF signaling molecules. DSF and BDSF positively regulate the biosynthesis of an antifungal factor (heat-stable antifungal factor, HSAF) in L. enzymogenes. Finally, we show that RpfFXc1 and RpfFOH11 have similar functions in regulating HSAF production in L. enzymogenes, as well as the virulence, synthesis of virulence factors, biofilm formation, and extracellular polysaccharide production in X. campestris. These findings reveal a previously uncharacterized mechanism of DSF-mediated regulation in both biocontrol and pathogenic bacteria. [ABSTRACT FROM AUTHOR]

Bicyclol, an innovative hepatoprotective drug, was approved by the Chinese National Medical Products Administration (NMPA) in 2001 to treat Hepatitis B and drug-induced liver injury. Two active metabolites of bicyclol have been identified as M2 and M3. To evaluate the impact on drug safety and efficacy of possible drug-drug interactions (DDIs) associated with these metabolites, a sufficient quantity of these metabolites is required. Herein, we report a concise novel route for the synthesis of M2 and M3 using the Suzuki-Miyaura coupling as the key step. Furthermore, we complete the gram-scale syntheses of M2 and M3. [ABSTRACT FROM AUTHOR]

Background: Soybean has long been utilized in the realm of traditional Chinese medicine. One of its extracts, soybean glycolipids, serves as a vital by-product of soybean oil refining, but its chemical composition and pharmacological potential have yet to be fully elucidated. Methods: In this study, the chemical components were isolated, and the inhibitory effects of these isolates were explored in different zebrafish inflammatory models by survival rate, Histological examination assay and quantitative Real-time PCR assay. The cytotoxicity of daidzin in RAW264.7 cells was evaluated by cell viability assay, and the effect of daidzin on the release of inflammatory cytokines in RAW264.7 cells was detected by enzyme linked immunosorbent assay (ELISA). Western blotting, immunofluorescence assay and alpha7 nicotinic acetylcholine receptors siRNA transfection assay were used to further explore the anti-inflammatory mechanism of daidzin. Results: Four compounds (verticilloside, soya-cerebroside I, soya-cerebroside II and daidzin) were firstly isolated from the soybean glycolipids, among which verticilloside and daidzin inhibited the lipopolysaccharide, CuSO4- and tail cut-stimulated zebrafish inflammation. Noticeably, daidzin exhibited anti-inflammatory activities by increasing the survival rate, alleviating the inflammatory cells infiltration, and down-regulating the expression of pro-inflammatory cytokines and nuclear factor kappa-B, NF-kappa-B inhibitor alpha, and signal transducer and activator of transcription3 in zebrafish. Moreover, daidzin decreased the secretion of IL-6 and TNF-α, inhibited the nuclear translocations of nuclear factor kappa-B p65 and p-signal transducer and activator of transcription3 as well as the NF-kappa-B inhibitor alpha phosphorylation at Ser32 in RAW 264.7 cells. More importantly, it elevated the expression level of alpha7 nicotinic acetylcholine receptors in both zebrafish and RAW 264.7 cells, and the inhibitory effect of daidzin was attenuated after the addition of alpha7 nicotinic acetylcholine receptors siRNA. Conclusion: Our study revealed that daidzin inhibited inflammation by activating the cholinergic anti-inflammatory pathway and further inhibiting the nuclear factor kappa-B and signal transducer and activator of transcription3 signaling. At the same time, it also promotes the recycling of crude soybean glycolipids and supports the potential use of daidzin as a functional food or natural dietary anti-inflammatory agent. [ABSTRACT FROM AUTHOR]

In this study, a hybrid dynamic model of high-speed thin-rimmed gears is developed. In this model, the translational and angular displacements (including the rigid and vibration displacements) with a total of six degrees-of-freedom (DOFs) are selected as the generalized coordinates for each gear, and the meshing force distributions along the contact line and between the teeth are considered. Thus, the model can be implemented under stationary and nonstationary conditions. The condensed finite element models (FEMs) are developed with the centrifugal and inertia forces for gear bodies. This paper proposes a novel method to couple the lumped-parameter model and condensed finite element model for the hybrid dynamic model system, which considers the variation of the meshing tooth during the gear operation, namely, the variations of the acting point of meshing force. Based on the model, the dynamic analysis of high-speed thin-rimmed gears is conducted under stationary speed and acceleration processes. The effects of the flexible gear body, high speed, and tooth errors on the system dynamics and tooth load distribution are investigated. The analysis results are also compared with the current reference and pure finite element method to validate the proposed model. [ABSTRACT FROM AUTHOR]

Among the extensive repertoire of carbohydrate-active enzymes, lytic polysaccharide monooxygenases (LPMOs) have a key role in recalcitrant biomass degradation. LPMOs are copper-dependent enzymes that catalyze oxidative cleavage of glycosidic bonds in polysaccharides such as cellulose and chitin. Several LPMOs contain carbohydrate-binding modules (CBMs) that are known to promote LPMO efficiency. However, structural and functional properties of some CBMs remain unknown, and it is not clear why some LPMOs, like CjLPMO10A from the soil bacterium Cellvibrio japonicus, have multiple CBMs (CjCBM5 and CjCBM73). Here, we studied substrate binding by these two CBMs to shine light on their functional variation and determined the solution structures of both by NMR, which constitutes the first structure of a member of the CBM73 family. Chitin-binding experiments and molecular dynamics simulations showed that, while both CBMs bind crystalline chitin with Kd values in the micromolar range, CjCBM73 has higher affinity for chitin than CjCBM5. Furthermore, NMR titration experiments showed that CjCBM5 binds soluble chitohexaose, whereas no binding of CjCBM73 to this chitooligosaccharide was detected. These functional differences correlate with distinctly different arrangements of three conserved aromatic amino acids involved in substrate binding. In CjCBM5, these residues show a linear arrangement that seems compatible with the experimentally observed affinity for single chitin chains. On the other hand, the arrangement of these residues in CjCBM73 suggests a wider binding surface that may interact with several chitin chains. Taken together, these results provide insight into natural variation among related chitin-binding CBMs and the possible functional implications of such variation. [ABSTRACT FROM AUTHOR]

C6H9N3O3, monoclinic, P21/n (no. 14), a = 9.5584(4) Å, b = 6.7828(3) Å, c = 12.8337(6) Å, β = 103.8810(10)∘, V = 807.74(6) Å3, Z = 4, Rgt(F) = 0.0455, wRref(F2) = 0.1166, T = 170.0 K. [ABSTRACT FROM AUTHOR]

C5H7N3O3, monoclinic, P21/n (no. 14), a = 8.7821(7) Å, b = 7.1320(5) Å, c = 11.6293(10) Å, β = 104.718 (3) ∘ , V = 704.49(10) Å3, Z = 4, R g t (F) = 0.0468, w R r e f (F2) = 0.1125, T = 170.0 K. [ABSTRACT FROM AUTHOR]

Global cropland expansion has been recognized as a key driver of food security. However, cropland expansion induced alterations in biophysical properties of the Earth's surface and greenhouse gas emissions may potentially impact the Earth's climate system. These changes could, in turn, affect cropland productivity and the potential distribution of croplands, although the underlying mechanisms remain relatively underexplored. In this study, a global climate model was employed to quantify the impact of global cropland expansion on cropping potential, utilizing observed and derived cropland expansion data. Our findings reveal that since 10000 BC, a 28% increase in cropland expansion has led to a 1.2% enhancement in global cropping potential, owing to more favorable precipitation and temperature conditions. This suggests that global cropland expansion yields dual benefits to crop production. However, in regions with low growth rates of cropping potential, cropland expansion proves to be an inefficient method for augmenting local crop potential yield. As croplands continue to expand worldwide, the capacity to support populations in different regions is altered, thereby reducing cropping potential inequality among nations. [ABSTRACT FROM AUTHOR]

Copper nanoparticle-based catalysts have been extensively applied in industry, but the nanoparticles tend to sinter into larger ones in the chemical atmospheres, which is detrimental to catalyst performance. In this work, we used dealuminated Beta zeolite to support copper nanoparticles (Cu/Beta-deAl) and showed that these particles become smaller in methanol vapor at 200°C, decreasing from ~5.6 to ~2.4 nanometers in diameter, which is opposite to the general sintering phenomenon. A reverse ripening process was discovered, whereby migratable copper sites activated by methanol were trapped by silanol nests and the copper species in the nests acted as new nucleation sites for the formation of small nanoparticles. This feature reversed the general sintering channel, resulting in robust catalysts for dimethyl oxalate hydrogenation performed with supported copper nanoparticles for use in industry. [ABSTRACT FROM AUTHOR]

Purpose: To compare the accuracy, safety, and consistency of asymmetric trapezoid and near-square side-port incision in cataract surgery. Setting: Aier Eye Hospital of Wuhan University, Wuhan, Hubei Province, China. Design: Prospective pilot study. Methods: This study included patients who underwent phacoemulsification between January 2022 and August 2022. They were divided into Group A and Group B using the random number table method. Group A was given a near-square side-port incision and Group B was given an asymmetric trapezoid side-port incision. We contrasted the differences in incision length, width, and shape; surgical time; and postoperative intraocular pressure (IOP) between the 2 groups. Results: 220 eyes of 220 patients were included. The mean external width of the incision in Group A was much smaller than that in Group B (P < .01), and the consistency of the incision diameter in Group A was better than that in Group B. There was no statistically significant difference in incision length between the 2 groups (P = .75). 1 day after surgery, there was no statistically significant difference in incision morphology between the 2 groups (P = .72). The operating time for Group A was significantly shorter than that of Group B (P < .01). There was no obvious incision leakage in both groups after surgery, and the IOP was generally elevated after surgery, but there was no significant statistical difference between the 2 groups (P = .98). Conclusions: The present study suggests that a near-square side-port results in better consistency of incision width and shorter surgical time. [ABSTRACT FROM AUTHOR]

Hepatoblastoma, a common extracranial malignant solid tumor in childhood, is often detected at an advanced stage and is difficult to treat surgically. Despite the availability of multiple comprehensive treatments that can be combined with surgery, hepatoblastoma treatment outcomes remain poor. Surgery is the main treatment strategy for hepatoblastoma, but it faces many challenges, including tumor attachment to surrounding tissues, tumor wrapping or invading of vital organs and tissues, the presence of giant or multiple tumors, distant metastasis, the formation of a tumor thrombus, and significant surgical trauma. In this review, we discuss recent research advances and propose potential strategies for overcoming these challenges. Such strategies may improve the rate of hepatoblastoma resection and local control in children, as well as reduce complications and trauma. [ABSTRACT FROM AUTHOR]

The proliferation and myogenic differentiation of muscle stem cells (MuSCs) are important factors affecting muscle development and beef quality. There is increasing evidence that circRNAs can regulate myogenesis. We found a novel circRNA, named circRRAS2 that is significantly upregulated in the differentiation phase of bovine MuSCs. Here, we aimed to determine its roles in the proliferation and myogenic differentiation of these cells. The results showed that circRRAS2 was expressed in several bovine tissues. CircRRAS2 inhibited MuSCs proliferation and promoted myoblast differentiation. In addition, chromatin isolation by using RNA purification and mass spectrometry in differentiated muscle cells identified 52 RNA-binding proteins that could potentially bind to circRRAS2, in order to regulate their differentiation. The results suggest that circRRAS2 could be a specific regulator of myogenesis in bovine muscle. CircRRAS2 expression is higher in DM cells than in GM cells. CircRRAS2 could significantly inhibit the proliferation and apoptosis of bovine MuSCs. CircRRAS2 promotes the differentiation of bovine MuSCs into myotubes. CircRRAS2 may exert regulatory effects through multiple RNA binding proteins. [ABSTRACT FROM AUTHOR]

Objective: Toll-like receptor 4 (TLR4) is crucial in induction of innate immune response through recognition of invading pathogens or endogenous alarming molecules. Ligand-triggered dimerization of TLR4 is essential for the activation of NF-κB and IRF3 through MyD88- or TRIF-dependent pathways. Saquinavir (SQV), an FDA-approved HIV protease inhibitor, has been shown to attenuate the activation of NF-κB induced by HMGB1 by blocking TLR4-MyD88 association in proteasome independent pathway. This study aims to define whether SQV is an HMGB1-specific and MyD88-dependent TLR4 signaling inhibitor and which precise signaling element of TLR4 is targeted by SQV. Materials and Methods: PMA differentiated human THP-1 macrophages or reconstituted HEK293 cells were pretreated with SQV before stimulated by different TLR agonists. TNF-a level was evaluated through ELISA assay. NF-κB activation was analyzed using NF-κB SEAP reporting system. The levels of MyD88/TRIF pathways-related factors were examined by immunoblot. TLR4 endocytosis was assessed by immunocytochemistry. TLR4 dimerization was determined using immunoprecipitation between different tagged TLR4 and an in silico molecular docking experiment was performed to explore the possible binding site of SQV on its target. Results: Our data showed that SQV suppresses both MyD88- and TRIF-dependent pathways in response to lipopolysaccharide (LPS), a critical sepsis inducer and TLR4 agonist, leading to downregulation of NF-κB and IRF3. SQV did not suppress MyD88-dependent pathway triggered by TLR1/2 agonist Pam3csk4. In the only TRIF-dependent pathway, SQV did not alleviate IRF3 phosphorylation induced by TLR3 agonist Poly(I:C). Furthermore, dimerization of TLR4 following LPS or HMGB1 stimulation was decreased by SQV. Conclusion: We concluded that TLR4 receptor complex is one of the mammalian targets of SQV, and TLR4-mediated immune responses and consequent risk for uncontrolled inflammation could be modulated by FDA-approved drug SQV. [ABSTRACT FROM AUTHOR]

While the detailed mechanisms for how particulate matter (PM) causes adverse health effects in the lungs remain largely unknown, endoplasmic reticulum (ER) stress has been implicated in PM-induced lung injury. The present study was undertaken to examine how/if ER stress might regulate PM-induced inflammation, and to begin to define potential underlying molecular mechanisms. Here, ER stress hallmarks were examined in human bronchial epithelial (HBE) cells exposed to PM. To confirm roles of certain pathways, siRNA targeting ER stress genes and an ER stress inhibitor were employed. Expression of select inflammatory cytokines and related signaling pathway components by the cells were assessed as well. The results showed that PM exposure induced elevations in two ER stress hallmarks, i.e. GRP78 and IRE1a, in time-and/or dose-related manners in the HBE cells. Inhibition of ER stress by siRNA for GRP78 or IRE1a significantly alleviated the PM-induced effects. Further, ER stress appeared to regulate PM-induced inflammation -- likely through downstream autophagy and NF-jB pathways -- as implied by studies showing that inhibition of ER stress by siRNA of GRP78 or IRE1a caused significant amelioration of PM-induced autophagy and subsequent activation of NF-jB pathways. Moreover, the ER stress inhibitor 4-PBA were used to confirm the protective effects against PM-induced outcomes. Together, the results suggest ER stress plays a deleterious role in PM-induced airway inflammation, possibly through activation of autophagy and NF-jB signaling. Accordingly, protocols/treatments that could lead to inhibited ER stress could potentially be effective for treatment of PM-related airway disorders. [ABSTRACT FROM AUTHOR]

BACKGROUND: 2,4,6-Trichlorophenol (TCP), 2,4,6-tribromophenol (TBP) and 2,4,6-triiodophenol (TIP) are three widely detected trihalophenolic disinfection by-products (DBPs). Previous studies have mainly focused on the carcinogenic risk and developmental toxicity of 2,4,6-trihalophenols. Very little is known about their immunotoxicity in mammals. OBJECTIVES: We investigated the effects of 2,4,6-trihalophenols on mammalian immunity using a mouse macrophage model infected with bacteria or intracellular parasites and aimed to elucidate the underlying mechanisms from an epitranscriptomic perspective. The identified mechanisms were further validated in human peripheral blood mononuclear cells (PBMCs). METHODS: The mouse macrophage cell line RAW264.7 and primary mouse peritoneal macrophages were exposed to different concentrations of TCP, TBP, and TIP. The pro-inflammatory marker Ly6C, the survival of the bacterium Escherichia coli (E. coli), and the parasite burden of Toxoplasma gondii (T. gondii) were assessed. Furthermore, the global gene expression profiling of macrophages following exposure to 2,4,6-trihalophenols was obtained through RNA-sequencing (RNA-seq). The effects of 2,4,6-trihalophenols on RNA 푁[sup 6]-methyladenosine (m[sup 6]A) methyltransferases and total RNA m[sup 6]A levels were evaluated using Western blotting and dot blot, respectively. Transcriptome-wide m[sup 6]A methylome was analyzed by m[sup 6]A-seq. In addition, expression of m[sup 6]A regulators and total RNA m[sup 6]A levels in human PBMCs exposed to 2,4,6-trihalophenols were detected using quantitative reverse transcriptase polymerase chain reaction and dot blot, respectively. RESULTS: Mouse macrophages exposed to TCP, TBP, or TIP had lower expression of the pro-inflammatory marker Ly6C, with a greater difference from control observed for TIP-exposed cells. Consistently, macrophages exposed to such DBPs, especially TIP, were susceptible to infection with the bacterium E. coli and the intracellular parasite T. gondii, indicating a compromised ability of macrophages to defend against pathogens. Intriguingly, macrophages exposed to TIP had significantly greater m[sup 6]A levels, which correlated with the greater expression levels of m[sup 6]A methyltransferases. Macrophages exposed to each of the three 2,4,6-trihalophenols exhibited transcriptome-wide redistribution of m[sup 6]A. In particular, the m[sup 6]A peaks in genes associated with immune-related pathways were altered after exposure. In addition, differences in m[sup 6]A were also observed in human PBMCs after exposure to 2,4,6-trihalophenols. DISCUSSION: These findings suggest that 2,4,6-trihalophenol exposure impaired the ability of macrophages to defend against pathogens. This response might be associated with notable differences in m[sup 6]A after exposure. To the best of our knowledge, this study presents the first m[sup 6]A landscape across the transcriptome of immune cells exposed to pollutants. However, significant challenges remain in elucidating the mechanisms by which m[sup 6]A mediates immune dysregulation in infected macrophages after 2,4,6-trihalophenol exposure. [ABSTRACT FROM AUTHOR]

Myelodysplastic syndrome (MDS) is a group of clonal hematopoietic neoplasms originating from hematopoietic stem progenitor cells (HSPCs). We previously identified frequent roundabout guidance receptor 1 (ROBO1) mutations in patients with MDS, while the exact role of ROBO1 in hematopoiesis remains poorly delineated. Here, we report that ROBO1 deficiency confers MDS-like disease with anemia and multilineage dysplasia in mice and predicts poor prognosis in patients with MDS. More specifically, Robo1 deficiency impairs HSPC homeostasis and disrupts HSPC pool, especially the reduction of megakaryocyte erythroid progenitors, which causes a blockage in the early stages of erythropoiesis in mice. Mechanistically, transcriptional profiling indicates that Cdc42, a member of the Rho-guanosine triphosphatase family, acts as a downstream target gene for Robo1 in HSPCs. Overexpression of Cdc42 partially restores the self-renewal and erythropoiesis of HSPCs in Robo1-deficient mice. Collectively, our result implicates the essential role of ROBO1 in maintaining HSPC homeostasis and erythropoiesis via CDC42. [ABSTRACT FROM AUTHOR]

A considerable number of lytic polysaccharide monooxygenases (LPMOs) and other carbohydrate-active enzymes are modular, with catalytic domains being tethered to additional domains, such as carbohydrate-binding modules, by flexible linkers. While such linkers may affect the structure, function, and stability of the enzyme, their roles remain largely enigmatic, as do the reasons for natural variation in length and sequence. Here, we have explored linker functionality using the two-domain cellulose-active ScLPMO10C from Streptomyces coelicolor as a model system. In addition to investigating the WT enzyme, we engineered three linker variants to address the impact of both length and sequence and characterized these using small-angle X-ray scattering, NMR, molecular dynamics simulations, and functional assays. The resulting data revealed that, in the case of ScLPMO10C, linker length is the main determinant of linker conformation and enzyme performance. Both the WT and a serine-rich variant, which have the same linker length, demonstrated better performance compared with those with either a shorter linker or a longer linker. A highlight of our findings was the substantial thermostability observed in the serine-rich variant. Importantly, the linker affects thermal unfolding behavior and enzyme stability. In particular, unfolding studies show that the two domains unfold independently when mixed, whereas the full-length enzyme shows one cooperative unfolding transition, meaning that the impact of linkers in biomass-processing enzymes is more complex than mere structural tethering [ABSTRACT FROM AUTHOR]

With the increasing prominence of data security in cloud storage, we propose a practical and robust cloud storage scheme, which uses quantum random numbers as encryption keys, disperses the keys using Shamir’s secret sharing scheme, applies erasure coding to the ciphertext, and securely transmits the data through quantum key distribution protected networks to the distributed clouds. This system offers several key advantages, including quantum-level security, fault tolerance, and storage space saving. To validate its feasibility, we conduct comprehensive experimental tests covering essential functionalities such as encryption/decryption, key preservation, and data storage. By successfully demonstrating the effectiveness of our proposal, we aim to accelerate the application of quantum technology in cloud storage. [ABSTRACT FROM AUTHOR]

Poor soil physical properties, serious salinization and low soil nutrients are the limiting factors for crop yield in saline-sodic soil. Long-term cattle manure application is an important measure that can affect the physicochemical properties and increase the maize yield of saline-sodic soil. This experiment included five treatments according to the history of cattle manure application: a control treatment with no cattle manure (CK) and treatments with cattle manure application for 14 years (14 a), 17 years (17 a), 20 years (20 a), and 25 years (25 a). The results indicated that compared with the CK treatment, longterm cattle manure application to saline-sodic soil resulted in significant increases in soil organic matter (SOM), soil total nitrogen (TN) and available nutrients at the 0-20 cm and 20-40 cm depths (p<0.05). The soil physical properties improved significantly, and cattle manure application significantly decreased the soil bulk density (ρb) and soil density (ρd) and increased the soil total porosity (ft) and water-holding capacity (WHC). With the number of years of cattle manure application, the soil pH, electrical conductivity (EC), exchangeable sodium percentage (ESP) and sodium adsorption ratio (SAR1:5) decreased significantly, and the maize yield gradually increased over time from 8690 kg/hm² in the CK treatment to 14 690 kg/hm² in the 25a treatment. There were significant differences among all treatments (p<0.05). The results showed that long-term cattle manure application decreased the soil ρb and saline-alkaline properties, which was the main factor that affected the maize yield in the saline-sodic soil, especially for soil ρb. [ABSTRACT FROM AUTHOR]

• Polymorphism of formed NiO nanoparticles with size in 25-50 nm was observed. • High toxicity of nanoparticles was witnessed against MG-63 cancer cells. • NiO nanoparticles revealed high biocompatibility on HEK293 normal cells. • Nanoparticles showed bactericidal activity on various human pathogenic bacteria. The green synthesis of metal oxide nanoparticles is interestingly becoming familiar as the reason of its non-toxicity, economical, eco-friendly, and commerciality. The secondary metabolites and bioactive molecules in biomaterial extract play an important role of reducing, capping, stabilizing and chelating activities to form the nanoparticles as a substitute of hazardous chemical surfactants. Hence this present work concentrated on the biogenic synthesis of NiO nanoparticles using grains extract of Oryza longistaminata (red rice), and their toxicity assessment as well as biomedical applications. The synthesized nanoparticles were widely characterized by using several analytical and biomedical techniques. The reduction mechanism of Ni2+ ions using major active phytocompound in red rice grains extract was stated. UV–visible spectroscopic analysis revealed the formation of NiO nanoparticles by emerging the sharp surface Plasmon resonance band at 326 nm. The absorption peaks of red rice grains extract at 206, 260, 330 and 410 nm stated the presence of phytocompounds in them. X-ray diffraction pattern explicated the high crystallinity of NiO nanoparticles and their average crystallite size was found to be at 36 nm. The diffraction pattern of red rice grains revealed the amorphous characteristic. Fourier transform infrared analysis displayed the different vibrational functional groups of nanoparticles and red rice grains. Dynamic light scattering analysis showed the particle size distribution was in between 30-65 nm. High-resolution transmission electron microscopic analysis showed the hexagonal, rectangular, oval and spherical shaped polymorphism of NiO nanoparticles and their size was in 25-50 nm. Energy dispersive X-ray spectrum and mapping analyses validated the formation of NiO nanoparticles by the major existence of Ni and O elements. The high toxicity of nanoparticles towards MG-63 osteosarcoma cancer cells was revealed at 94.69% in 100 μg/mL concentration. The nanoparticles showed no severe toxicity against HEK293 normal cells and red blood cells till higher concentration. The biomedical analyses such as antioxidant, anti-inflammatory and anti-diabetic activities exposed the potential bio-drug activity of NiO nanoparticles with maximum activity of 86.22, 84.23 and 85.18% in the high concentration of 80 μg/mL. The antibacterial activity divulged the good bactericidal drug nature of NiO nanoparticles against both Gram-positive (S. aureus and B. Subtilis) and Gram-negative (S. typhi and K. pneumonia) bacteria with low MBC/MIC ratio. As per the assessment of results, the biosynthesized NiO nanoparticles have non-carcinogenic, high biocompatible and potent nature and it suggests that they can be used as therapeutic candidates in biomedical, clinical and pharmaceutical fields for treating various diseases. [Display omitted] [ABSTRACT FROM AUTHOR]

A novel synthetic protocol for the direct oxidation of alkanes, including cyclic and linear ones, to give ketones and alcohols using hydrogen peroxide as terminal oxidant under ambient conditions is presented. The active catalyst for this challenging transformation is conveniently generated by combination of simple Fe salts with N‐methyl bis(picolylamine) (Me‐bpa). Utilizing picolinic acid as additive leads to improved yields of ketones and alcohols (32‐57 %). The reaction can be conveniently scaled up to multi‐g scale. [ABSTRACT FROM AUTHOR]

Purpose: The relationship between contrast-enhanced ultrasound (CEUS) hemodynamics and the molecular biomarkers of adult-type diffuse gliomas, particularly isocitrate dehydrogenase (IDH), remains unclear. This study was conducted to provide a comprehensive description of the vascularization of adult-type diffuse gliomas using quantitative indicators. Additionally, it was designed to identify any variables with the potential to intraoperatively predict IDH mutation status. Methods: This prospective study enrolled patients with adult-type diffuse gliomas between November 2021 and September 2022. Intraoperative CEUS was performed, and CEUS videos were recorded for 90-second periods. Hemodynamic parameters, including the peak enhancement (PE) difference, were calculated based on the time-intensity curve of the region of interest. A differential analysis was performed on the CEUS parameters with respect to molecular biomarkers and grades. Receiver operating characteristic curves for various parameters were analyzed to evaluate the ability of those parameters to predict IDH mutation status. Results: Sixty patients with adult-type diffuse gliomas were evaluated. All hemodynamic parameters, apart from rising time, demonstrated significant differences between IDH-mutant and IDH-wildtype adult-type diffuse gliomas. The PE difference emerged as the optimal indicator for differentiating between IDH-wildtype and IDH-mutant gliomas, with an area under the curve of 0.958 (95% confidence interval, 0.406 to 0.785). Additionally, the hemodynamic parameters revealed significant differences across both grades and types of adult-type diffuse gliomas. Conclusion: Hemodynamic parameters can be used intraoperatively to effectively distinguish between IDH-wildtype and IDH-mutant adult-type diffuse gliomas. Additionally, quantitative CEUS equips neurosurgeons with dynamic perfusion information for various types and grades of adult-type diffuse gliomas. [ABSTRACT FROM AUTHOR]

Modern pollen datasets are essential for pollen-based quantitative paleoclimate (e.g. precipitation) reconstructions, which can aid to better understand recent climate change and its underlying forcing mechanisms. A modern pollen dataset based on surface sediments from 90 lakes in the shrub, meadow, steppe and desert regions of the central and western Tibetan Plateau (TP) was established to fill geographical gaps left by previous datasets. Ordination analyses of pollen data and climatic parameters revealed that annual precipitation is the dominant factor for modern pollen distribution on the central and western TP. A regional transfer function for annual precipitation was developed with the weighted averaging partial least squares (WAPLS), which suggests a good inference power of the modern pollen dataset for annual precipitation. A case study in which the transfer function was effectively applied to a fossil pollen record from Lake Tangra Yumco on the central TP for paleoprecipitation reconstruction demonstrated the significance of the modern pollen dataset in less data region for paleoclimate change studies. Data from this study are openly available via the Zenodo portal (Ma et al., 2023; https://doi.org/10.5281/zenodo.8008474). [ABSTRACT FROM AUTHOR]

A new species, Achalinus sheni sp. nov., from central Hunan Province is described, based on the results of molecular systematics and morphological characters according to five specimens. Our molecular phylogeny inferred from the mitochondrial CO1 gene fragment revealed that this new species is most closely related to A. yunkaiensis, but a considerable amount of genetic divergence exists between them (p-distance ranging from 5.8% to 6.4%) and much distinct genetic divergence exists compared with other known Achalinus species (p-distance ranging from 10.4% to 15.8%), supporting its validity. Morphologically, it can be distinguished from its congeners by: (1) dorsal scales strongly keeled, 23 rows throughout the body, the outmost row smooth and significantly enlarged; (2) tail relatively short, TaL/TL 0.183 ~ 0.224; (3) the suture between internasals subequal to the suture between prefrontals; (4) loreal one, subrectangular, LorH/LorL 0.53 ~ 0.57; (5) ventrals 161-170, anal entire, subcaudals 55-61, not paired; (6) the length of supraocular equal to or longer than the length of upper anterior temporal; and (7) vertebral line inconspicuous and subcaudal streak absent. Currently, 27 species of Achalinus are known in the world, amongst which 20 species are distributed in China. Moreover, a key to species of the genus Achalinus is provided in this study. [ABSTRACT FROM AUTHOR]

Neuroendocrine prostate cancer (NEPC) is a lethal subtype of prostate cancer. It is characterized by the loss of androgen receptor (AR) signaling in neuroendocrine transdifferentiation, and finally, resistance to AR-targeted therapy. With the application of a new generation of potent AR inhibitors, the incidence of NEPC is gradually increasing. The molecular mechanism of neuroendocrine differentiation (NED) after androgen deprivation therapy (ADT) remains largely unclear. In this study, using NEPC-related genome sequencing database analyses, we screened RACGAP1, a common differentially expressed gene. We investigated RACGAP1 expression in clinical prostate cancer specimens by IHC. Regulated pathways were examined by Western blotting, qRT-PCR, luciferase reporter, chromatin immunoprecipitation, and immunoprecipitation assays. The corresponding function of RACGAP1 in prostate cancer was analyzed by CCK-8 and Transwell assays. The changes of neuroendocrine markers and AR expression in C4-2-R and C4-2B-R cells were detected in vitro. We confirmed that RACGAP1 contributed to NE transdifferentiation of prostate cancer. Patients with high tumor RACGAP1 expression had shorter relapse-free survival time. The expression of RACGAP1 was induced by E2F1. RACGAP1 promoted neuroendocrine transdifferentiation of prostate cancer by stabilizing EZH2 expression in the ubiquitin-proteasome pathway. Moreover, overexpression of RACGAP1 promoted enzalutamide resistance of castration-resistant prostate cancer (CRPC) cells. Our results showed that the upregulation of RACGAP1 by E2F1 increased EZH2 expression, which drove NEPC progression. This study explored the molecular mechanism of NED and may provide novel methods and ideas for targeted therapy of NEPC. [ABSTRACT FROM AUTHOR]

The novel protein elicitor GP1pro is the protein component of glycoprotein GP‐1 isolated and identified from Streptomyces kanasensis ZX01. GP1pro induces the production of reactive oxygen species (ROS) and a hypersensitive response (HR), along with the accumulation of resistance‐related genes and secondary metabolites. It ultimately regulates plant defence responses. Further analysis revealed that GP1pro interacts with the PIP2‐family aquaporin protein NbPIP2;4 on the plant plasma membrane (PM) in Nicotiana benthamiana. PM localization is necessary for inducing GP1pro resistance. These results demonstrate that NbPIP2;4 acts as a H2O2 transporter to positively regulate plant immunity and ROS accumulation. In summary, this study elucidates a conserved and novel pathway caused by GP1pro to initiate host cellular defences by targeting the plant aquaporin protein NbPIP2;4 and transporting apoplast‐to‐cytoplast H2O2 to regulate plant immunity. Summary Statement: We found a protein elicitor from actinomycetes that interacts with the tobacco aquaporin NbPIP2;4 to regulate plant immunity and enhance tobacco disease resistance. This provides a basis for the development of protein elicitors for green plant protection strategies. [ABSTRACT FROM AUTHOR]

In this study, the fast response of East Asian summer precipitation to COVID-19–induced aerosol emission reductions is examined using the Community Earth System Model, version 2.2 (CESM2.2). The emission reductions decreased aerosol optical depth and cloud cover over northern China in June 2020. The troposphere became warmer, strengthening the land–sea thermal contrast and anomalous southerly winds. The subtropical westerly jet accelerated and shifted southward, favoring low-level convergence, upward air motions, and subsequent condensational heating over the Yangtze River basin (YRB). The feedback of condensational heating in return strengthened the convergence and ascent. The western North Pacific subtropical high was intensified, which further enhanced the moisture advection and convergence over the YRB. Both the enhanced moisture convergence and ascent increased precipitation over the YRB during June 2020. Furthermore, local and remote emission reductions show different impacts on convection and moisture transport over the YRB. The emission reductions over China caused stronger convective precipitation (1.15 vs 0.63 mm day-1 ) but weaker larger-scale precipitation (1.17 vs 2.24 mm day-1 ) than the emission reductions outside China. In addition to the emission reductions, the sea surface temperature (SST) anomalies in 2020 also play an important role in increasing precipitation over the YRB, contributing about 42.8%. The relative contribution of SST anomalies also increases under the COVID-19–induced emission scenario. [ABSTRACT FROM AUTHOR]