49 results on '"Ji, Jun"'
Search Results
2. Diagnostic value of peripheral TiM-3, NT proBNP, and Sestrin2 testing in left-to-right shunt congenital heart disease with heart failure
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Wang, Qianqian, Liu, Guotao, Teng, Yan, Feng, Xing, Chen, Zhiyun, Wang, Fen, Gu, Yuan, Jia, Lishan, Cao, Ji Jun, and Lu, Zhong Xing
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- 2023
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- View/download PDF
3. Biochemical and transcriptomic analyses of the symbiotic interaction between Cremastra appendiculata and the mycorrhizal fungus Coprinellus disseminatus
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Gao, Yanyan, Ji, Jun, Zhang, Yujin, Yang, Ningxian, and Zhang, Mingsheng
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- 2022
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4. Molecular evolutionary process of advanced gastric cancer during sequential chemotherapy detected by circulating tumor DNA
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Xi, Wenqi, Zhou, Chenfei, Xu, Fei, Sun, Debin, Wang, Shengzhou, Chen, Yawei, Ji, Jun, Ma, Tao, Wu, Junwei, Shangguan, Chengfang, Zhu, Zhenggang, and Zhang, Jun
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- 2022
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- View/download PDF
5. Common DNA methylation changes in biliary tract cancers identify subtypes with different immune characteristics and clinical outcomes
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Qiu, Zhiquan, Ji, Jun, Xu, Yu, Zhu, Yan, Gao, Chunfang, Wang, Guoqiang, Li, Chengcheng, Zhang, Yuzi, Zhao, Jing, Wang, Chenyang, Wen, Xiaofang, Zhang, Zhou, Li, Bingsi, Zhang, Zhihong, Cai, Shangli, Li, Bin, and Jiang, Xiaoqing
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- 2022
- Full Text
- View/download PDF
6. The effect of obstructive jaundice on the sensitivity of intravenous anesthetic of remimazolam: study protocol for a controlled multicenter trial
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Liu, Wen, Yang, Bin, Ji, Jun-Wei, Yang, Hua, Song, Hong-Hao, Qiu, Hai-Bo, and Song, Jin-Chao
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- 2022
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7. Can paraspinal muscle degeneration be a reason for refractures after percutaneous kyphoplasty? A magnetic resonance imaging observation
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Zhao, He, He, Yan, Yang, Jun-Song, Bao, Wei, Chen, Jian, Liu, Ji-Jun, Li, Qing-Da, Liu, Peng, Qian, Bing, Zhao, Yuan-Ting, and Hao, Ding-Jun
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- 2021
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8. STAT3/HIF-1α signaling activation mediates peritoneal fibrosis induced by high glucose
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Yang, Xiaoxiao, Bao, Manchen, Fang, Yi, Yu, Xiaofang, Ji, Jun, and Ding, Xiaoqiang
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- 2021
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9. KK-LC-1 may be an effective prognostic biomarker for gastric cancer
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Ji, Jun, Chen, Jiahui, Wang, Anqiang, Zhang, Wei, Ju, Hongge, Liu, Yang, and Li, Leping
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- 2021
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10. Retraction Note to: Injectable hydrogel encapsulating Cu2MnS2 nanoplates for photothermal therapy against breast cancer
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Fu, Ji‑jun, Chen, Ming‑ yue, Li, Jie‑xia, Zhou, Jun‑ hua, Xie, Sheng‑nan, Yuan, Ping, Tang, Bo, and Liu, Cheng‑ cheng
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- 2021
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- View/download PDF
11. Increased circulating level of interleukin-6 and CD8+ T cell exhaustion are associated with progression of COVID-19
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Yang, Peng-Hui, Ding, Yi-Bo, Xu, Zhe, Pu, Rui, Li, Ping, Yan, Jin, Liu, Ji-Luo, Meng, Fan-Ping, Huang, Lei, Shi, Lei, Jiang, Tian-Jun, Qin, En-Qiang, Zhao, Min, Zhang, Da-Wei, Zhao, Peng, Yu, Ling-Xiang, Wang, Zhao-Hai, Hong, Zhi-Xian, Xiao, Zhao-Hui, Xi, Qing, Zhao, De-Xi, Yu, Peng, Zhu, Cai-Zhong, Chen, Zhu, Zhang, Shao-Geng, Ji, Jun-Sheng, Wang, Fu-Sheng, and Cao, Guang-Wen
- Published
- 2020
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12. The microneedles carrying cisplatin and IR820 to perform synergistic chemo-photodynamic therapy against breast cancer
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Fu, Ji-jun, Li, Chu-wen, Liu, Yang, Chen, Ming-yue, Zhang, Qiang, Yu, Xi-yong, Wu, Bo, Li, Jie-xia, Du, Ling-ran, Dang, Yuan-ye, Wu, Dan, Wei, Min-yan, Lin, Zhi-qiang, and Lei, Xue-ping
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- 2020
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13. Bismuth chelate as a contrast agent for X-ray computed tomography
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Fu, Ji-jun, Guo, Jun-jie, Qin, Ai-ping, Yu, Xi-yong, Zhang, Qiang, Lei, Xue-ping, Huang, Yu-gang, Chen, Ming-yue, Li, Jie-xia, Zhang, Yu, Liu, Jing-ping, Dang, Yuan-ye, Wu, Dan, Zhao, Xiao-ya, Lin, Zhong-xiao, Lin, Yin-lei, Li, Song-pei, and Zhang, Ling-yan
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- 2020
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14. Preparation, characterization and in vitro–in vivo evaluation of bortezomib supermolecular aggregation nanovehicles
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Chen, Ming-yue, Xiao, Ze-kuan, Lei, Xue-ping, Li, Jie-xia, Yu, Xi-yong, Zhang, Jian-ye, Ye, Guo-dong, Guo, Yu-juan, Mo, Guangquan, Li, Chu-wen, Zhang, Yu, Zhang, Ling-min, Lin, Zhi-qiang, and Fu, Ji-jun
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- 2020
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15. Phase II study of R–CVP followed by rituximab maintenance therapy for patients with advanced marginal zone lymphoma: consortium for improving survival of lymphoma (CISL) study
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Oh, Sung Yong, Kim, Won Seog, Kim, Jin Seok, Kim, Seok Jin, Yoon, Dok Hyun, Yang, Deok-Hwan, Lee, Won Sik, Kim, Hyo Jung, Yhim, Ho-Young, Jeong, Seong Hyun, Won, Jong Ho, Lee, Suee, Kong, Jee Hyun, Lim, Sung-Nam, Ji, Jun Ho, Kwon, Kyung A., Lee, Gyeong-Won, Lee, Jae Hoon, Lee, Ho Sup, Shin, Ho-Jin, and Suh, Cheolwon
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- 2019
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16. Novel polymerase spiral reaction assay for the visible molecular detection of porcine circovirus type 3
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Ji, Jun, Xu, Xin, Wang, Xueyu, Zuo, Kejing, Li, Zhili, Leng, Chaoliang, Kan, Yunchao, Yao, Lunguang, and Bi, Yingzuo
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- 2019
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17. A positive feedback between IDO1 metabolite and COL12A1 via MAPK pathway to promote gastric cancer metastasis
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Xiang, Zhen, Li, Jun, Song, Shuzheng, Wang, Jiexuan, Cai, Wei, Hu, Wenjun, Ji, Jun, Zhu, Zhenggang, Zang, Lu, Yan, Ranlin, and Yu, Yingyan
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- 2019
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18. Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method
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Wang, Xueyu, Xu, Xin, Hu, Wen, Zuo, Kejing, Li, Zhili, Kan, Yunchao, Yao, Lunguang, Ji, Jun, and Bi, Yingzuo
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- 2019
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- View/download PDF
19. QTL for spike-layer uniformity and their influence on yield-related traits in wheat
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Zhao, Chunhua, Zhang, Na, Wu, Yongzhen, Sun, Han, Liu, Cheng, Fan, Xiaoli, Yan, Xuemei, Xu, Hongxing, Ji, Jun, and Cui, Fa
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- 2019
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20. Autoantibody detection is not recommended for chronic pancreatitis: a cross-sectional Study of 557 patients
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Zeng, Xiang-Peng, Liu, Ting-Ting, Hao, Lu, Xin, Lei, Wang, Teng, He, Lin, Pan, Jun, Wang, Dan, Bi, Ya-Wei, Ji, Jun-Tao, Liao, Zhuan, Du, Ting-Ting, Lin, Jin-Huan, Zhang, Di, Guo, Hong-Lei, Chen, Hui, Zou, Wen-Bin, Li, Bai-Rong, Cong, Zhi-Jie, Wang, Li-Sheng, Xu, Zheng-Lei, Xie, Ting, Liu, Ming-Hao, Deng, An-Mei, Li, Zhao-Shen, and Hu, Liang-Hao
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- 2019
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21. Risk factor for steatorrhea in pediatric chronic pancreatitis patients
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Hao, Lu, Wang, Teng, He, Lin, Bi, Ya-Wei, Zhang, Di, Zeng, Xiang-Peng, Xin, Lei, Pan, Jun, Wang, Dan, Ji, Jun-Tao, Du, Ting-Ting, Lin, Jin-Huan, Wang, Li-Sheng, Zou, Wen-Bin, Chen, Hui, Xie, Ting, Guo, Hong-Lei, Li, Bai-Rong, Liao, Zhuan, Xu, Zheng-Lei, Li, Zhao-Shen, and Hu, Liang-Hao
- Published
- 2018
- Full Text
- View/download PDF
22. RETRACTED ARTICLE: Injectable hydrogel encapsulating Cu2MnS2 nanoplates for photothermal therapy against breast cancer
- Author
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Fu, Ji-jun, Chen, Ming-yue, Li, Jie-xia, Zhou, Jun-hua, Xie, Sheng-nan, Yuan, Ping, Tang, Bo, and Liu, Cheng-cheng
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- 2018
- Full Text
- View/download PDF
23. N-glycosylation of serum proteins for the assessment of patients with IgD multiple myeloma.
- Author
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Jie Chen, Meng Fang, Xiaoling Chen, Changhong Yi, Jun Ji, Cheng Cheng, Mengmeng Wang, Xing Gu, Quansheng Sun, Chunfang Gao, Chen, Jie, Fang, Meng, Chen, Xiaoling, Yi, Changhong, Ji, Jun, Cheng, Cheng, Wang, Mengmeng, Gu, Xing, Sun, Quansheng, and Gao, Chunfang
- Subjects
GLYCOSYLATION ,MULTIPLE myeloma ,NUCLEOTIDE sequence ,PROGRESSION-free survival ,INDIVIDUALIZED medicine - Abstract
Background: Because glycosylation is one of the most common post-translational modifications of proteins and because changes in glycosylation have been shown to have a significant correlation with the development of many cancer types, we investigated the serum N-glycome used to diagnose, stage and evaluate the pathological outcomes in IgD multiple myeloma.Methods: Serum samples were available for 20 patients with IgD multiple myeloma, 41 patients with light chain multiple myeloma and 42 healthy control subjects. Serum N-glycans were released and analysed using DNA sequencer-assisted fluorophore-assisted capillary electrophoresis.Results: Characteristic changes were revealed in the serum N-glycome of IgD myeloma. In particular, three N-glycans (NG1(6)A2F, Peak3; NG1(3)A2F, Peak4; NA2FB, Peak7) showed increased clinical value. The best area under the ROC curve of NG1(6)A2F to diagnose IgD myeloma was 0.981, with a 95.0% sensitivity and 95.2% specificity, and that of NG1(3)A2F was 0.936, with a 95.0% sensitivity and 78.6% specificity. The best area under the ROC curve of NA2FB/NG1(3)A2F to differentially diagnose IgD myeloma versus light chain myeloma was 0.744, with a 95.3% sensitivity and 50.0% specificity. The level of NG1(3)A2F was correlated with the international staging system, while the higher abundance of NA2FB presented in IgD myeloma was predictive of a shorter progression-free survival.Conclusions: The advent of serum N-glycan signatures may play a role in the diagnosis, staging and prognosis of IgD myeloma and will serve as the foundation for a precision medicine approach to this rare subtype of multiple myeloma. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
24. Epidermal growth factor receptor inhibitor ameliorates excessive astrogliosis and improves the regeneration microenvironment and functional recovery in adult rats following spinal cord injury.
- Author
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Zai-Wang Li, Ji-Jun Li, Lan Wang, Jian-Ping Zhang, Jing-Jing Wu, Xu-Qiang Mao, Guo-Feng Shi, Qian Wang, Feng Wang, and Jian Zou
- Subjects
- *
EPIDERMAL growth factor receptors , *SPINAL cord injuries , *BONE regeneration , *GLIAL fibrillary acidic protein , *VIMENTIN - Abstract
Background Astrogliosis is a common phenomenon after spinal cord injury (SCI). Although this process exerts positive effects on axonal regeneration, excessive astrogliosis imparts negative effects on neuronal repair and recovery. Epidermal growth factor receptor (EGFR) pathway is critical to the regulation of reactive astrogliosis, and therefore is a potential target of therapeutics to better control the response. In this report, we aim to investigate whether blocking EGFR signaling using an EGFR tyrosine kinase specific inhibitor can attenuate reactive astrogliosis and promote functional recovery after a traumatic SCI. Method The astrocyte scratch injury model in vitro and the weight-drop SCI model in vivo were used as model systems. PD168393 was used to inhibit EGFR signaling activation. Astrocytic activation and phosphorylated EGFR (pEGFR) were observed after immunofluorescence staining and Western blot analysis. The rate of proliferation was determined by immunofluorescence detection of BrdU-incorporating cells located next to the wound. The levels of TNF-a, iNOS, COX-2 and IL-1ß in the culture medium under different conditions were assayed by ELISA. Western blot was performed to semi-quantify the expression of EGFR/pEGFR, glial fibrillary acid protein (GFAP) and chondroitin sulfate proteoglycans (CSPGs). Myelin was stained by Luxol Fast Blue Staining. Cresyl violet eosin staining was performed to analyze the lesion cavity volume and neuronal survival following injury. Finally, functional scoring and residual urine recording were performed to show the rats’ recovery. Results EGFR phosphorylation was found to parallel astrocyte activation, and EGFR inhibitor PD168393 potently inhibited scratch-induced reactive astrogliosis and proinflammatory cytokine/mediator secretion of reactive astrocytes in vitro. Moreover, local administration of PD168393 in the injured area suppressed CSPGs production and glial scar formation, and resulted in reduced demyelination and neuronal loss, which correlated with remarkable hindlimb motor function and bladder improvement in SCI rats. Conclusions The specific EGFR inhibitor PD168393 can ameliorate excessive reactive astrogliosis and facilitate a more favorable environment for axonal regeneration after SCI. As such, EGFR inhibitor may be a promising therapeutic intervention in CNS injury. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
25. Association between metabolic abnormalities and HBV related hepatocelluar carcinoma in Chinese: a cross-sectional study.
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Zhao, Jinyan, Zhao, Yunpeng, Wang, Hao, Gu, Xing, Ji, Jun, and Gao, Chunfang
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LIVER cancer ,METABOLIC disorders ,BODY mass index ,BLOOD sugar ,BLOOD lipids ,CHINESE people - Abstract
Background: Previous studies suggested that the abnormality of metabolism is a newly identified risk factor in HBV-related hepatocellular carcinoma (HCC). The association between metabolic factors and hepatocellular carcinoma (HCC) has not been clarified up to now. This study was conducted to investigate the prevalence of metabolic abnormalities in HCC and to probe the association between metabolic parameters and liver function as well, so as to evaluate the interactions between metabolism and the development of HBV-related HCC.Methods: Totally 179 cases of HBV-related HCC, who were surgically treated and pathologically confirmed were enrolled. HBV carriers (n = 100) and healthy controls (n = 150) were recruited from routine physical examination during the same period. Body mass index (BMI) was obtained from medical documentation. All the metabolic-related parameters and liver function tests were determined with routine biochemical or immunological analytic methods. Malondialdehyde (MDA) and total antioxidant capacity(TAOC)were detected by chemical analytic methods. A stratified analysis was conducted according to BMI, glycated albumin (GA), free fatty acids (FFA), and the relationships between the metabolic-related parameters and liver functions were analyzed in HCC and control subjects.Results: HCC group showed significantly high levels of mean BMI, serum glucose, low serum lipids levels than controls (P < 0.05). Acquired by stratified analysis, the higher the BMI, the higher level of insulin and homeostasis model assessment for insulin resistance (HOMA-IR) (P < 0.01) were found in HCC patients. Elevated level of MDA and γ-glutamyltransferase (GGT) were revealed in those with high serum FFA level for the first time. Strong associations between metabolic factors and liver function were shown in HCC (P < 0.05). Higher GA level was strongly associated with increased risk of cancer compared to healthy controls (OR = 9.87, 95% confidence interval: 1.86~52.29). Serum triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C) levels were negative contributory factors for HCC (OR = 0.05, 95% confidence interval: 0.01~0.27 and OR = 0.32, 95% confidence interval, 0.11~0.95: respectively).Conclusions: Metabolic abnormalities are closely associated with the occurrence and development of HBV-related HCC. Oxidative stress and/or lipid peroxidation might be involved in the pathogenesis and acceleration of liver function impairments in HCC. [ABSTRACT FROM AUTHOR]- Published
- 2011
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- View/download PDF
26. Injectable hydrogel encapsulating Cu2MnS2 nanoplates for photothermal therapy against breast cancer.
- Author
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Fu, Ji-jun, Chen, Ming-yue, Li, Jie-xia, Zhou, Jun-hua, Xie, Sheng-nan, Yuan, Ping, Tang, Bo, and Liu, Cheng-cheng
- Subjects
- *
HYDROGELS , *ENCAPSULATION (Catalysis) , *MANGANESE-copper alloys , *NANOSTRUCTURED materials , *PHOTOTHERMAL effect , *BREAST cancer treatment - Abstract
Background: In order to explore the possibility of treating breast cancer by local photo-therapy, a photothermal agents loaded in situ hydrogel was established. In detail, The Cu2MnS2 nanoplates were prepared by one-pot synthesis and, the thermosensitive Pluronic F127 was used as the hydrogel matrix. The Cu2MnS2 nanoplates and the hydrogel were characterized by morphous, particle size, serum stability, photothermal performance upon repeated 808 nm laser irradiation as well as the rheology features. The therapeutic effects of the Cu2MnS2 nanoplates and the hydrogel were evaluated qualitatively and quantitatively in 4T1 mouse breast cancer cells. The retention, photothermal efficacy, therapeutic effects and systemic toxicity of the hydrogel were assessed in tumor bearing mouse model. Results: The Cu2MnS2 nanoplates with a diameter of about 35 nm exhibited satisfying serum stability, photo-heat conversion ability and repeated laser exposure stability. The hydrogel encapsulation did not negatively influence the above features of the photothermal agent. The nanoplates loaded in situ hydrogel shows a phase transition at body temperature and, as a result, a long retention in vivo. Conclusions: The photothermal agent embedded hydrogel played a promising photothermal therapeutic effects in tumor bearing mouse model with low systemic toxicity after peritumoral administration. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
27. Epidermal growth factor receptor inhibitor ameliorates excessive astrogliosis and improves the regeneration microenvironment and functional recovery in adult rats following spinal cord injury.
- Author
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Li, Zai-Wang, Li, Ji-Jun, Wang, Lan, Zhang, Jian-Ping, Wu, Jing-Jing, Mao, Xu-Qiang, Shi, Guo-Feng, Wang, Qian, Wang, Feng, and Zou, Jian
- Abstract
Background: Astrogliosis is a common phenomenon after spinal cord injury (SCI). Although this process exerts positive effects on axonal regeneration, excessive astrogliosis imparts negative effects on neuronal repair and recovery. Epidermal growth factor receptor (EGFR) pathway is critical to the regulation of reactive astrogliosis, and therefore is a potential target of therapeutics to better control the response. In this report, we aim to investigate whether blocking EGFR signaling using an EGFR tyrosine kinase specific inhibitor can attenuate reactive astrogliosis and promote functional recovery after a traumatic SCI.Method: The astrocyte scratch injury model in vitro and the weight-drop SCI model in vivo were used as model systems. PD168393 was used to inhibit EGFR signaling activation. Astrocytic activation and phosphorylated EGFR (pEGFR) were observed after immunofluorescence staining and Western blot analysis. The rate of proliferation was determined by immunofluorescence detection of BrdU-incorporating cells located next to the wound. The levels of TNF-α, iNOS, COX-2 and IL-1β in the culture medium under different conditions were assayed by ELISA. Western blot was performed to semi-quantify the expression of EGFR/pEGFR, glial fibrillary acid protein (GFAP) and chondroitin sulfate proteoglycans (CSPGs). Myelin was stained by Luxol Fast Blue Staining. Cresyl violet eosin staining was performed to analyze the lesion cavity volume and neuronal survival following injury. Finally, functional scoring and residual urine recording were performed to show the rats' recovery.Results: EGFR phosphorylation was found to parallel astrocyte activation, and EGFR inhibitor PD168393 potently inhibited scratch-induced reactive astrogliosis and proinflammatory cytokine/mediator secretion of reactive astrocytes in vitro. Moreover, local administration of PD168393 in the injured area suppressed CSPGs production and glial scar formation, and resulted in reduced demyelination and neuronal loss, which correlated with remarkable hindlimb motor function and bladder improvement in SCI rats.Conclusions: The specific EGFR inhibitor PD168393 can ameliorate excessive reactive astrogliosis and facilitate a more favorable environment for axonal regeneration after SCI. As such, EGFR inhibitor may be a promising therapeutic intervention in CNS injury. [ABSTRACT FROM AUTHOR]- Published
- 2014
- Full Text
- View/download PDF
28. Integrating multiple 'omics' analyses identifies serological protein biomarkers for preeclampsia.
- Author
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Liu, Linda Y, Yang, Ting, Ji, Jun, Wen, Qiaojun, Morgan, Alexander A, Jin, Bo, Chen, Gongxing, Lyell, Deirdre J, Stevenson, David K, Ling, Xuefeng B, and Butte, Atul J
- Abstract
Background: Preeclampsia (PE) is a pregnancy-related vascular disorder which is the leading cause of maternal morbidity and mortality. We sought to identify novel serological protein markers to diagnose PE with a multi-'omics' based discovery approach.Methods: Seven previous placental expression studies were combined for a multiplex analysis, and in parallel, two-dimensional gel electrophoresis was performed to compare serum proteomes in PE and control subjects. The combined biomarker candidates were validated with available ELISA assays using gestational age-matched PE (n=32) and control (n=32) samples. With the validated biomarkers, a genetic algorithm was then used to construct and optimize biomarker panels in PE assessment.Results: In addition to the previously identified biomarkers, the angiogenic and antiangiogenic factors (soluble fms-like tyrosine kinase (sFlt-1) and placental growth factor (PIGF)), we found 3 up-regulated and 6 down-regulated biomakers in PE sera. Two optimal biomarker panels were developed for early and late onset PE assessment, respectively.Conclusions: Both early and late onset PE diagnostic panels, constructed with our PE biomarkers, were superior over sFlt-1/PIGF ratio in PE discrimination. The functional significance of these PE biomarkers and their associated pathways were analyzed which may provide new insights into the pathogenesis of PE. [ABSTRACT FROM AUTHOR]- Published
- 2013
- Full Text
- View/download PDF
29. Analysis of factors related to death of severe acute pancreatitis.
- Author
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Chen Ji-jun, Li Chao, Wang Yan-jun, Zhao Bing-gang, Han Qiang, and Yin Wen
- Subjects
- *
PANCREATITIS - Abstract
An abstract to the article "Analysis of factors related to death of severe acute pancreatitis," by Chen Ji-jun and colleagues is presented.
- Published
- 2012
- Full Text
- View/download PDF
30. Overexpression of epithelial growth factor receptor (EGFR) predicts better response to neo-adjuvant chemotherapy in patients with triple-negative breast cancer.
- Author
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Tang, Yiqing, Zhu, Li, Li, Yafen, Ji, Jun, Li, Jianfang, Yuan, Fei, Wang, Dengbin, Chen, Weiguo, Huang, Ou, Chen, Xiaosong, Wu, Jiayi, Shen, Kunwei, Loo, Wings Ty, Chow, Louis Wc, Loo, Wings T Y, and Chow, Louis W C
- Abstract
Background: Triple negative breast cancer (TNBC) occurs in approximately 10% to 25% of all patients with breast cancer and is associated with poor prognosis. Neo-adjuvant chemotherapy has been reported to produce a higher pathologic complete response (pCR) rate in TNBC. If pCR is achieved, patients with TNBC had a similar survival with non-TNBC patients. The aim of our study was to investigate the protein expression of epithelial growth factor receptor (EGFR) and response to neo-adjuvant chemotherapy and clinical outcome in patients with TNBC compared with non-TNBC.Methods: A total of 198 locally advanced breast cancer patients who received neo-adjuvant chemotherapy were studied. Immunohistochemistry (IHC) was carried out to detect the protein expression of EGFR in tumor samples. Clinical and pathological parameters, pCR rate and survival data were compared between 40 TNBCs and 158 non-TNBCs.Results: In 198 cases who received neo-adjuvant chemotherapy, significant differences exist in surgical therapy (P=0.005) and pCR rate (P=0.012) between patients with TNBCs and non-TNBCs. Overexpression of EGFR was significantly associated with pCR rate in patients with TNBCs (P < 0.001). Survival analysis revealed that patients with TNBCs had worse DFS and OS than those with non-TNBCs (P = 0.001, P < 0.001 respectively). Furthermore, for patients with non-TNBCs, those who achieved pCR had better DFS and OS than those who achieved RD (both P < 0.001).Conclusions: Our results suggested that patients with TNBCs had increased pCR rates compared with non-TNBC. Overexpression of EGFR predicted better response to neo-adjuvant chemotherapy in patients with TNBCs. [ABSTRACT FROM AUTHOR]- Published
- 2012
- Full Text
- View/download PDF
31. Injectable hydrogel encapsulating Cu 2 MnS 2 nanoplates for photothermal therapy against breast cancer.
- Author
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Fu JJ, Chen MY, Li JX, Zhou JH, Xie SN, Yuan P, Tang B, and Liu CC
- Subjects
- Animals, Cell Line, Tumor, Female, Mammary Neoplasms, Animal pathology, Mice, Inbred BALB C, Nanoparticles ultrastructure, Poloxamer chemistry, Copper chemistry, Hydrogels chemistry, Hyperthermia, Induced, Injections, Mammary Neoplasms, Animal therapy, Manganese chemistry, Nanoparticles chemistry, Phototherapy, Sulfides chemistry
- Abstract
Background: In order to explore the possibility of treating breast cancer by local photo-therapy, a photothermal agents loaded in situ hydrogel was established. In detail, The Cu
2 MnS2 nanoplates were prepared by one-pot synthesis and, the thermosensitive Pluronic F127 was used as the hydrogel matrix. The Cu2 MnS2 nanoplates and the hydrogel were characterized by morphous, particle size, serum stability, photothermal performance upon repeated 808 nm laser irradiation as well as the rheology features. The therapeutic effects of the Cu2 MnS2 nanoplates and the hydrogel were evaluated qualitatively and quantitatively in 4T1 mouse breast cancer cells. The retention, photothermal efficacy, therapeutic effects and systemic toxicity of the hydrogel were assessed in tumor bearing mouse model., Results: The Cu2 MnS2 nanoplates with a diameter of about 35 nm exhibited satisfying serum stability, photo-heat conversion ability and repeated laser exposure stability. The hydrogel encapsulation did not negatively influence the above features of the photothermal agent. The nanoplates loaded in situ hydrogel shows a phase transition at body temperature and, as a result, a long retention in vivo., Conclusions: The photothermal agent embedded hydrogel played a promising photothermal therapeutic effects in tumor bearing mouse model with low systemic toxicity after peritumoral administration.- Published
- 2018
- Full Text
- View/download PDF
32. N-glycosylation of serum proteins for the assessment of patients with IgD multiple myeloma.
- Author
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Chen J, Fang M, Chen X, Yi C, Ji J, Cheng C, Wang M, Gu X, Sun Q, and Gao C
- Subjects
- Case-Control Studies, Female, Follow-Up Studies, Glycosylation, Humans, Male, Middle Aged, Multiple Myeloma blood, Prognosis, Protein Processing, Post-Translational, Survival Rate, Biomarkers, Tumor blood, Blood Proteins analysis, Immunoglobulin D blood, Multiple Myeloma diagnosis, Polysaccharides blood, Polysaccharides chemistry
- Abstract
Background: Because glycosylation is one of the most common post-translational modifications of proteins and because changes in glycosylation have been shown to have a significant correlation with the development of many cancer types, we investigated the serum N-glycome used to diagnose, stage and evaluate the pathological outcomes in IgD multiple myeloma., Methods: Serum samples were available for 20 patients with IgD multiple myeloma, 41 patients with light chain multiple myeloma and 42 healthy control subjects. Serum N-glycans were released and analysed using DNA sequencer-assisted fluorophore-assisted capillary electrophoresis., Results: Characteristic changes were revealed in the serum N-glycome of IgD myeloma. In particular, three N-glycans (NG1(6)A2F, Peak3; NG1(3)A2F, Peak4; NA2FB, Peak7) showed increased clinical value. The best area under the ROC curve of NG1(6)A2F to diagnose IgD myeloma was 0.981, with a 95.0% sensitivity and 95.2% specificity, and that of NG1(3)A2F was 0.936, with a 95.0% sensitivity and 78.6% specificity. The best area under the ROC curve of NA2FB/NG1(3)A2F to differentially diagnose IgD myeloma versus light chain myeloma was 0.744, with a 95.3% sensitivity and 50.0% specificity. The level of NG1(3)A2F was correlated with the international staging system, while the higher abundance of NA2FB presented in IgD myeloma was predictive of a shorter progression-free survival., Conclusions: The advent of serum N-glycan signatures may play a role in the diagnosis, staging and prognosis of IgD myeloma and will serve as the foundation for a precision medicine approach to this rare subtype of multiple myeloma.
- Published
- 2017
- Full Text
- View/download PDF
33. The effectiveness of long-needle acupuncture at acupoints BL30 and BL35 for CP/CPPS: a randomized controlled pilot study.
- Author
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Zhou M, Yang M, Chen L, Yu C, Zhang W, Ji J, Chen C, Shen X, and Ying J
- Subjects
- Acupuncture Points, Adult, Humans, Male, Middle Aged, Needles, Pilot Projects, Treatment Outcome, Young Adult, Acupuncture Therapy instrumentation, Chronic Pain therapy, Pelvic Pain therapy, Prostatitis therapy
- Abstract
Background: The chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is one of the commonest chronic inflammatory diseases in adult men, for which acupuncture has been used to relieve related symptoms. The present study aimed to evaluate the therapeutic effect of the long-needle acupuncture on CP/CPPS., Methods: A randomized traditional acupuncture-controlled single blind study was conducted on 77 patients who were randomized into long-needle acupuncture (LA) and traditional acupuncture (TA) groups. The patients received six sessions of acupuncture for 2 weeks and a follow-up was scheduled at week 24. The primary outcome was measured by the total National Institutes of Health-Chronic Prostatitis Symptom Index (NIH-CPSI) score at week 2. Four domains of the NIH-CPSI (urination, pain or discomfort, effects of symptoms, and quality of life) and the clinical efficacy score served as the secondary outcome., Results: The total NIH-CPSI score at week 2 and week 24 was significantly improved in the LA group compared with the TA group. LA significantly improved urination, pain or discomfort, the effects of symptoms, and the quality of life at week 2 and week 24 and patients undergoing LA treatment had a higher clinical efficacy score., Conclusion: Needling at the BL30 and BL35 using LA benefits patients with CP/CPPS., Trial Registration: The study was registered at the Chinese Clinical Trial Register ( ChiCTR-ICR-15006138 ).
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- 2017
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34. Prospective stratification of patients at risk for emergency department revisit: resource utilization and population management strategy implications.
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Jin B, Zhao Y, Hao S, Shin AY, Wang Y, Zhu C, Hu Z, Fu C, Ji J, Wang Y, Zhao Y, Jiang Y, Dai D, Culver DS, Alfreds ST, Rogow T, Stearns F, Sylvester KG, Widen E, and Ling XB
- Subjects
- Adolescent, Adult, Aged, Child, Child, Preschool, Female, Forecasting, Humans, Infant, Male, Middle Aged, Prospective Studies, Retrospective Studies, Risk Assessment methods, Young Adult, Emergency Service, Hospital statistics & numerical data, Patient Readmission trends
- Abstract
Background: Estimating patient risk of future emergency department (ED) revisits can guide the allocation of resources, e.g. local primary care and/or specialty, to better manage ED high utilization patient populations and thereby improve patient life qualities., Methods: We set to develop and validate a method to estimate patient ED revisit risk in the subsequent 6 months from an ED discharge date. An ensemble decision-tree-based model with Electronic Medical Record (EMR) encounter data from HealthInfoNet (HIN), Maine's Health Information Exchange (HIE), was developed and validated, assessing patient risk for a subsequent 6 month return ED visit based on the ED encounter-associated demographic and EMR clinical history data. A retrospective cohort of 293,461 ED encounters that occurred between January 1, 2012 and December 31, 2012, was assembled with the associated patients' 1-year clinical histories before the ED discharge date, for model training and calibration purposes. To validate, a prospective cohort of 193,886 ED encounters that occurred between January 1, 2013 and June 30, 2013 was constructed., Results: Statistical learning that was utilized to construct the prediction model identified 152 variables that included the following data domains: demographics groups (12), different encounter history (104), care facilities (12), primary and secondary diagnoses (10), primary and secondary procedures (2), chronic disease condition (1), laboratory test results (2), and outpatient prescription medications (9). The c-statistics for the retrospective and prospective cohorts were 0.742 and 0.730 respectively. Total medical expense and ED utilization by risk score 6 months after the discharge were analyzed. Cluster analysis identified discrete subpopulations of high-risk patients with distinctive resource utilization patterns, suggesting the need for diversified care management strategies., Conclusions: Integration of our method into the HIN secure statewide data system in real time prospectively validated its performance. It promises to provide increased opportunity for high ED utilization identification, and optimized resource and population management.
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- 2016
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35. MTA2 enhances colony formation and tumor growth of gastric cancer cells through IL-11.
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Zhou C, Ji J, Cai Q, Shi M, Chen X, Yu Y, Zhu Z, and Zhang J
- Subjects
- Apoptosis genetics, Cell Line, Tumor, Cell Movement genetics, Cell Proliferation genetics, Gene Expression Regulation, Neoplastic, Histone Deacetylases genetics, Humans, Interleukin-11 metabolism, Neoplasm Invasiveness genetics, Neoplasm Invasiveness pathology, Repressor Proteins genetics, Stomach Neoplasms pathology, Cell Transformation, Neoplastic genetics, Histone Deacetylases biosynthesis, Interleukin-11 genetics, Repressor Proteins biosynthesis, Stomach Neoplasms genetics
- Abstract
Background: We have preliminarily reported MTA2 expression in gastric cancer and its biological functions by using knockdown cell models, while the molecular mechanisms of MTA2 in regulating malignant behaviors are still unclear., Methods: MTA2 overexpression models were established by transfection assay in gastric cancer cells BGC-823 and MKN28. Cell proliferation assay, colony formation in soft agar, wound-healing assay and transwell migration assay were performed with MTA2 overexpression and negative control (NC) cells. Subcutaneous xenografts and pulmonary metastasis models by BGC-823/MTA2 and BGC-823/NC cells were used to observe the capacity of growth and metastasis in vivo. Differential gene expression in MTA2 knockdown and overexpression cells was analyzed by microarrays. IL-11, which demonstrated as differential expression in microarray, was detected by real-time PCR, western blot, ELISA and immunohistochemistry staining. Recombinant human IL-11 (rhIL-11) was administrated in cell proliferation and colony formation as rescue assay., Results: The numbers of colonies in soft agar were significantly more in BGC-823/MTA2 and MKN28/MTA2 cells, comparing with those in their NC cells. Capabilities of cell proliferation, wound-healing and cell migration were not significantly changed in MTA2 overexpression cells. The sizes of subcutaneous xenografts and pulmonary metastases of BGC-832/MTA2 cells were significantly larger than those in BGC-823/NC group. Differential expression of IL-11 was identified by genome expression microarray both in MTA2 knockdown and overexpression cells. IL-11 expression was elevated in BGC-823/MTA2 cells, whereas reduced in SGC-7901/shMTA2 cells. Administration of rhIL-11 recovered colony formation capacity of SGC-7901/shMTA2 cells., Conclusions: MTA2 overexpression enhances colony formation and tumor growth of gastric cancer cells, but not plays important role in cancer cell migration and metastasis. IL-11 is one of the downstream effectors of MTA2 in regulating gastric cancer cells growth.
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- 2015
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36. The Ebola epidemic is ongoing in West Africa and responses from China are positive.
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Zhao JM, Dong SJ, Li J, and Ji JS
- Abstract
The ongoing Ebola outbreak poses an alarming risk to the countries of West Africa and beyond. On August 8, 2014, the World Health Organization (WHO) declared the cross-country Ebola outbreak a Public Emergency of International Concern. China has had no confirmed cases of Ebola. In this paper, virologic characteristics, pathogenesis, clinical manifestations, laboratory examination and prophylactic vaccines and therapeutic drugs of Ebola are summarized. Importantly, active responses and actions from China are introduced. Moreover, the key issues in the future prevention and control of Ebola were also addressed.
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- 2015
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37. MAE-FMD: multi-agent evolutionary method for functional module detection in protein-protein interaction networks.
- Author
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Ji JZ, Jiao L, Yang CC, Lv JW, and Zhang AD
- Subjects
- Fungal Proteins metabolism, Humans, Yeasts metabolism, Algorithms, Computational Biology methods, Evolution, Molecular, Protein Interaction Mapping methods
- Abstract
Background: Studies of functional modules in a Protein-Protein Interaction (PPI) network contribute greatly to the understanding of biological mechanisms. With the development of computing science, computational approaches have played an important role in detecting functional modules., Results: We present a new approach using multi-agent evolution for detection of functional modules in PPI networks. The proposed approach consists of two stages: the solution construction for agents in a population and the evolutionary process of computational agents in a lattice environment, where each agent corresponds to a candidate solution to the detection problem of functional modules in a PPI network. First, the approach utilizes a connection-based encoding scheme to model an agent, and employs a random-walk behavior merged topological characteristics with functional information to construct a solution. Next, it applies several evolutionary operators, i.e., competition, crossover, and mutation, to realize information exchange among agents as well as solution evolution. Systematic experiments have been conducted on three benchmark testing sets of yeast networks. Experimental results show that the approach is more effective compared to several other existing algorithms., Conclusions: The algorithm has the characteristics of outstanding recall, F-measure, sensitivity and accuracy while keeping other competitive performances, so it can be applied to the biological study which requires high accuracy.
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- 2014
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38. Evaluation of the benefit of using blue dye in addition to indocyanine green fluorescence for sentinel lymph node biopsy in patients with breast cancer.
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Guo W, Zhang L, Ji J, Gao W, Liu J, and Tong M
- Subjects
- Adult, Aged, Breast Neoplasms surgery, Carcinoma, Ductal, Breast surgery, Female, Follow-Up Studies, Humans, Middle Aged, Neoplasm Staging, Prognosis, Prospective Studies, Breast Neoplasms diagnosis, Carcinoma, Ductal, Breast diagnosis, Coloring Agents, Indocyanine Green, Lymph Nodes pathology, Rosaniline Dyes, Sentinel Lymph Node Biopsy
- Abstract
Background: Near infrared-guided indocyanine green (ICG) fluorescence has vast potential for guiding sentinel lymph node biopsy (SLNB) in patients with breast cancer. The purpose of this study was to evaluate any additional clinical benefit for SLNB when blue dye is used in combination with ICG., Methods: Between November of 2009 and September of 2013, 86 patients diagnosed with breast cancer were investigated by SLNB using a combination of patent blue and ICG. A lymph node was considered as the sentinel lymph node (SLN) when it was stained with blue dye and/or fluorescence. A levelIandIIaxillary dissection was performed for verification of axillary node status after the SLNB., Results: The SLN identification rate of SLN for ICG-patent blue combination was comparable to that for ICG alone (98.8% versus 93%; P = 0.054), but the false-negative rate was reduced from 12% (3/25) to 4% (1/25). Twenty-four patients had positive SLNs. In two of those patients, although there were SLNs identified by both tracers, the positive SLNs were identified by blue dye only., Conclusion: Although blue dye did not improve the identification rate significantly, there was a definite benefit in improving the false-negative rate. The use of a fluorescence method together with blue dye is an ideal method for hospitals that do not have access to conventional radiation-based detection methods.
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- 2014
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39. A novel genetic map of wheat: utility for mapping QTL for yield under different nitrogen treatments.
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Cui F, Fan X, Zhao C, Zhang W, Chen M, Ji J, and Li J
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- Breeding, DNA, Plant genetics, Genetic Linkage, Genetic Markers, Microsatellite Repeats, Phenotype, Triticum metabolism, Chromosome Mapping, Nitrogen metabolism, Quantitative Trait Loci, Triticum genetics
- Abstract
Background: Common wheat (Triticum aestivum L.) is one of the most important food crops worldwide. Wheat varieties that maintain yield (YD) under moderate or even intense nitrogen (N) deficiency can adapt to low input management systems. A detailed genetic map is necessary for both wheat molecular breeding and genomics research. In this study, an F6:7 recombinant inbred line population comprising 188 lines was used to construct a novel genetic map and subsequently to detect quantitative trait loci (QTL) for YD and response to N stress., Results: A genetic map consisting of 591 loci distributed across 21 wheat chromosomes was constructed. The map spanned 3930.7 cM, with one marker per 6.7 cM on average. Genomic simple sequence repeat (g-SSR), expressed sequence tag-derived microsatellite (e-SSR), diversity arrays technology (DArT), sequence-tagged sites (STS), sequence-related amplified polymorphism (SRAP), and inter-simple sequence repeat (ISSR) molecular markers were included in the map. The linear relationships between loci found in the present map and in previously compiled physical maps were presented, which were generally in accordance. Information on the genetic and physical positions and allele sizes (when possible) of 17 DArT, 50 e-SSR, 44 SRAP, five ISSR, and two morphological markers is reported here for the first time. Seven segregation distortion regions (SDR) were identified on chromosomes 1B, 3BL, 4AL, 6AS, 6AL, 6BL, and 7B. A total of 22 and 12 QTLs for YD and yield difference between the value (YDDV) under HN and the value under LN were identified, respectively. Of these, QYd-4B-2 and QYddv-4B, two major stable QTL, shared support interval with alleles from KN9204 increasing YD in LN and decreasing YDDV. We probe into the use of these QTLs in wheat breeding programs. Moreover, factors affecting the SDR and total map length are discussed in depth., Conclusions: This novel map may facilitate the use of novel markers in wheat molecular breeding programs and genomics research. Moreover, QTLs for YD and YDDV provide useful markers for wheat molecular breeding programs designed to increase yield potential under N stress.
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- 2014
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40. MTA2 promotes gastric cancer cells invasion and is transcriptionally regulated by Sp1.
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Zhou C, Ji J, Cai Q, Shi M, Chen X, Yu Y, Liu B, Zhu Z, and Zhang J
- Subjects
- Actin Cytoskeleton metabolism, Adenocarcinoma mortality, Adenocarcinoma secondary, Animals, Binding Sites, Cell Line, Tumor, Cell Movement, Gene Expression Regulation, Neoplastic, Histone Deacetylases metabolism, Humans, Lung Neoplasms metabolism, Lung Neoplasms secondary, Male, Mice, Mice, Inbred BALB C, Mice, Nude, Neoplasm Invasiveness, Neoplasm Transplantation, Promoter Regions, Genetic, Repressor Proteins metabolism, Stomach Neoplasms mortality, Stomach Neoplasms pathology, Transcription, Genetic, Adenocarcinoma metabolism, Histone Deacetylases genetics, Lung Neoplasms genetics, Repressor Proteins genetics, Sp1 Transcription Factor physiology, Stomach Neoplasms genetics, Stomach Neoplasms metabolism
- Abstract
Background: MTA2 gene belongs to metastasis associated family, and is highly expressed in some solid tumors, including gastric cancer. Its biological function in gastric cancer is currently undefined., Methods: Metastasis-associated tumor gene family 2 (MTA2) and transcription factor specificity protein 1 (Sp1) expression were detected in 127 gastric cancer samples by immunohistochemistry staining. SGC-7901 and AGS gastric cancer cell lines transfected by MTA2 shRNA was used for biological function investigation. Binding and regulation activities of Sp1 on MTA2 promoter were investigated by chromatin immunoprecipitation and luciferase reporter gene., Results: The expression rate of MTA2 in gastric cancer tissues was 55.9% (71/127), and its expression was closely related to the depth of tumor invasion, lymph nodes metastasis, and TNM staging. MTA2 knockdown in human SGC-7901 and AGS gastric cancer cells significantly inhibited migration and invasion in vitro, and disrupted structure of cytoskeleton. MTA2 knockdown also attenuated xenografts growth and lung metastasis in nude mice model. MTA2 expression was positively correlated with transcription factor Sp1 in gastric cancer tissues (r = 0.326, P < 0.001). Sp1 bound to human MTA2 gene promoter at region from -1043 bp to -843 bp. Transcriptional activity of MTA2 promoter could be enhanced by Sp1 overexpression., Conclusions: MTA2 knockdown impairs invasion and metastasis of gastric cancer cells, and attenuates xenografts growth in vivo. Sp1 regulates MTA2 expression at transcriptional level.
- Published
- 2013
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41. Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo.
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Kallon S, Li X, Ji J, Chen C, Xi Q, Chang S, Xue C, Ma J, Xie Q, and Zhang Y
- Abstract
This study investigated the humoral immunization of Astragalus polysaccharide (APS) against H9N2 avian influenza virus (H9N2 AIV) infection in chickens.The effects of APS treatment on H9N2 infection was evaluated by an MTT [3(4, 5-dimethylthiazol-2-yl)-2, 3-diphenyl tetrazolium bromide] assay and analysis of MHC and cytokine mRNA expression. The effect on lymphocyte and serum antibody titers in vivo was also investigated. IL-4, IL-6, IL-10, LITAF, IL-12 and antibody titers to H9N2 AIV were enhanced in the first week after APS treatment. The results indicated that APS treatment reduces H9N2 AIV replication and promotes early humoral immune responses in young chickens.
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- 2013
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42. Cloud-based solution to identify statistically significant MS peaks differentiating sample categories.
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Ji J, Ling J, Jiang H, Wen Q, Whitin JC, Tian L, Cohen HJ, and Ling XB
- Subjects
- Algorithms, Area Under Curve, Biomarkers metabolism, Data Interpretation, Statistical, Humans, Internet, Proteome, Reproducibility of Results, Statistics as Topic, Computational Biology methods, Mass Spectrometry methods, Proteomics methods
- Abstract
Background: Mass spectrometry (MS) has evolved to become the primary high throughput tool for proteomics based biomarker discovery. Until now, multiple challenges in protein MS data analysis remain: large-scale and complex data set management; MS peak identification, indexing; and high dimensional peak differential analysis with the concurrent statistical tests based false discovery rate (FDR). "Turnkey" solutions are needed for biomarker investigations to rapidly process MS data sets to identify statistically significant peaks for subsequent validation., Findings: Here we present an efficient and effective solution, which provides experimental biologists easy access to "cloud" computing capabilities to analyze MS data. The web portal can be accessed at http://transmed.stanford.edu/ssa/., Conclusions: Presented web application supplies large scale MS data online uploading and analysis with a simple user interface. This bioinformatic tool will facilitate the discovery of the potential protein biomarkers using MS.
- Published
- 2013
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43. Phylogenetic diversity and genotypic complexity of H1N1 subtype swine influenza viruses isolated in mainland China.
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Liu Y, Wang J, Ji J, Chang S, Xue C, Ma J, Bi Y, and Xie Q
- Subjects
- Animals, China, Databases, Genetic, Evolution, Molecular, Hemagglutinin Glycoproteins, Influenza Virus genetics, Humans, Influenza A Virus, H1N1 Subtype genetics, Influenza A Virus, H1N1 Subtype isolation & purification, Orthomyxoviridae Infections virology, RNA-Dependent RNA Polymerase genetics, Receptors, Virus genetics, Sequence Analysis, Protein, Swine virology, Time Factors, Viral Proteins genetics, Genes, Viral, Genetic Variation, Genotype, Influenza A Virus, H1N1 Subtype classification, Phylogeny
- Abstract
Background: After the occurrence of 2009 pandemic H1N1, close attention has been paid to the H1N1 subtype swine influenza viruses (H1N1 SIV) by scientific communities in many countries. A large-scale sequence analysis of the NCBI Influenza Virus Resource Database on H1N1 SIVs submitted primarily by scientists in China during 1992 to 2011 was performed. The aims of this study were to elucidate the genetic and evolutionary characteristics of H1N1 SIVs, to identify and unify the lineages and genetic characteristics of the H1N1 SIVs isolated in mainland China., Results: Most of the strains were isolated during the period of 2008 to 2010 from Guangdong and Shandong provinces, China. Based on the phylogenetic and genotypic analyses, all of the H1N1 SIV strains can be classified into 8 lineages and 10 genotypes. All strains were of the characteristics of low pathogenic influenza viruses. The viruses of different lineage are characterized with different amino acid residues at the receptor-binding sites. Viruses containing PB2 genes of the classical swine, early seasonal human and recent seasonal human lineage might be more infectious to human. Some genotypes were directly related with human influenza viruses, which include strains that harbored genes derived from human influenza viruses., Conclusions: Phylogenetic diversity and complexity existed in H1N1 SIVs isolated in mainland China. These H1N1 SIV strains were closely related to other subtype influenza viruses, especially to human influenza viruses. Moreover, it was shown that, novel lineages and genotypes of H1N1 SIVs emerged recently in mainland China. These findings provided new and essential information for further understanding of the genetic and evolutionary characteristics and monitoring the H1N1 SIVs in mainland China.
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- 2012
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44. A diagnostic algorithm combining clinical and molecular data distinguishes Kawasaki disease from other febrile illnesses.
- Author
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Ling XB, Lau K, Kanegaye JT, Pan Z, Peng S, Ji J, Liu G, Sato Y, Yu TT, Whitin JC, Schilling J, Burns JC, and Cohen HJ
- Subjects
- Adult, Area Under Curve, Biomarkers blood, Biomarkers metabolism, Biomarkers urine, Diagnosis, Differential, Female, Fever blood, Fever diagnosis, Fever genetics, Fever urine, Gene Expression Profiling methods, Humans, Male, Middle Aged, Mucocutaneous Lymph Node Syndrome blood, Mucocutaneous Lymph Node Syndrome genetics, Mucocutaneous Lymph Node Syndrome urine, Multivariate Analysis, Proteinuria blood, Proteinuria diagnosis, Proteinuria urine, Proteomics methods, ROC Curve, Transcriptome, Algorithms, Diagnosis, Computer-Assisted methods, Mucocutaneous Lymph Node Syndrome diagnosis
- Abstract
Background: Kawasaki disease is an acute vasculitis of infants and young children that is recognized through a constellation of clinical signs that can mimic other benign conditions of childhood. The etiology remains unknown and there is no specific laboratory-based test to identify patients with Kawasaki disease. Treatment to prevent the complication of coronary artery aneurysms is most effective if administered early in the course of the illness. We sought to develop a diagnostic algorithm to help clinicians distinguish Kawasaki disease patients from febrile controls to allow timely initiation of treatment., Methods: Urine peptidome profiling and whole blood cell type-specific gene expression analyses were integrated with clinical multivariate analysis to improve differentiation of Kawasaki disease subjects from febrile controls., Results: Comparative analyses of multidimensional protein identification using 23 pooled Kawasaki disease and 23 pooled febrile control urine peptide samples revealed 139 candidate markers, of which 13 were confirmed (area under the receiver operating characteristic curve (ROC AUC 0.919)) in an independent cohort of 30 Kawasaki disease and 30 febrile control urine peptidomes. Cell type-specific analysis of microarrays (csSAM) on 26 Kawasaki disease and 13 febrile control whole blood samples revealed a 32-lymphocyte-specific-gene panel (ROC AUC 0.969). The integration of the urine/blood based biomarker panels and a multivariate analysis of 7 clinical parameters (ROC AUC 0.803) effectively stratified 441 Kawasaki disease and 342 febrile control subjects to diagnose Kawasaki disease., Conclusions: A hybrid approach using a multi-step diagnostic algorithm integrating both clinical and molecular findings was successful in differentiating children with acute Kawasaki disease from febrile controls.
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- 2011
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45. Epidemiology and immunoprotection of nephropathogenic avian infectious bronchitis virus in southern China.
- Author
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Xie Q, Ji J, Xie J, Chen F, Cai M, Sun B, Xue C, Ma J, and Bi Y
- Subjects
- Animals, Antibodies, Viral blood, China, Coronavirus Infections epidemiology, Coronavirus Infections prevention & control, Poultry, Poultry Diseases prevention & control, Viral Vaccines administration & dosage, Coronavirus Infections veterinary, Infectious bronchitis virus immunology, Infectious bronchitis virus isolation & purification, Poultry Diseases epidemiology, Poultry Diseases virology, Viral Vaccines immunology
- Abstract
Background: In last three years, 96 suspected poultry farms from different provinces in China were diagnosed for avian infectious bronchitis (IB) survey. Finally, 221 IBV strains were confirmed by dwarf embryo test and RT-PCR assay. By virus recovery trials, 187 of the isolates caused the birds died or distressed from nephritis, which was accordant with the clinical record., Results: Based on epidemiology analysis of recent field isolates of nephropathogenic IB in vaccinated farms in China, YL6 strain were used for vaccination and evaluated by antibody titer and challenge tests. The immunoprotection test indicated that the practical application of vaccine based on the recent field strains could finely facilitate controlling the nephropathogenic IB., Conclusions: Our study was aim at setting a guide for safeguard against nephropathogenic IBV-caused disease in China.
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- 2011
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46. Subchronic inhalation toxicity of gold nanoparticles.
- Author
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Sung JH, Ji JH, Park JD, Song MY, Song KS, Ryu HR, Yoon JU, Jeon KS, Jeong J, Han BS, Chung YH, Chang HK, Lee JH, Kim DW, Kelman BJ, and Yu IJ
- Subjects
- Administration, Inhalation, Animals, Blood drug effects, Blood Chemical Analysis, Body Weight, Eating drug effects, Female, Humans, Kidney pathology, Kidney physiology, Lung pathology, Lung physiology, Male, Materials Testing, Metal Nanoparticles chemistry, Metal Nanoparticles ultrastructure, Organ Size, Particle Size, Rats, Rats, Sprague-Dawley, Tissue Distribution, Gold administration & dosage, Gold toxicity, Metal Nanoparticles administration & dosage, Metal Nanoparticles toxicity
- Abstract
Background: Gold nanoparticles are widely used in consumer products, including cosmetics, food packaging, beverages, toothpaste, automobiles, and lubricants. With this increase in consumer products containing gold nanoparticles, the potential for worker exposure to gold nanoparticles will also increase. Only a few studies have produced data on the in vivo toxicology of gold nanoparticles, meaning that the absorption, distribution, metabolism, and excretion (ADME) of gold nanoparticles remain unclear., Results: The toxicity of gold nanoparticles was studied in Sprague Dawley rats by inhalation. Seven-week-old rats, weighing approximately 200 g (males) and 145 g (females), were divided into 4 groups (10 rats in each group): fresh-air control, low-dose (2.36 × 104 particle/cm3, 0.04 μg/m3), middle-dose (2.36 × 105 particle/cm3, 0.38 μg/m3), and high-dose (1.85 × 106 particle/cm3, 20.02 μg/m3). The animals were exposed to gold nanoparticles (average diameter 4-5 nm) for 6 hours/day, 5 days/week, for 90-days in a whole-body inhalation chamber. In addition to mortality and clinical observations, body weight, food consumption, and lung function were recorded weekly. At the end of the study, the rats were subjected to a full necropsy, blood samples were collected for hematology and clinical chemistry tests, and organ weights were measured. Cellular differential counts and cytotoxicity measurements, such as albumin, lactate dehydrogenase (LDH), and total protein were also monitored in a cellular bronchoalveolar lavage (BAL) fluid. Among lung function test measurements, tidal volume and minute volume showed a tendency to decrease comparing control and dose groups during the 90-days of exposure. Although no statistically significant differences were found in cellular differential counts, histopathologic examination showed minimal alveoli, an inflammatory infiltrate with a mixed cell type, and increased macrophages in the high-dose rats. Tissue distribution of gold nanoparticles showed a dose-dependent accumulation of gold in only lungs and kidneys with a gender-related difference in gold nanoparticles content in kidneys., Conclusions: Lungs were the only organ in which there were dose-related changes in both male and female rats. Changes observed in lung histopathology and function in high-dose animals indicate that the highest concentration (20 μg/m3) is a LOAEL and the middle concentration (0.38 μg/m3) is a NOAEL for this study., (© 2011 Sung et al; licensee BioMed Central Ltd.)
- Published
- 2011
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47. Phylogenetic distribution and predominant genotype of the avian infectious bronchitis virus in China during 2008-2009.
- Author
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Ji J, Xie J, Chen F, Shu D, Zuo K, Xue C, Qin J, Li H, Bi Y, Ma J, and Xie Q
- Subjects
- Animals, Chickens, China epidemiology, Coronavirus Infections epidemiology, Coronavirus Infections virology, Genotype, Infectious bronchitis virus isolation & purification, Molecular Sequence Data, Pandemics, Poultry Diseases epidemiology, Coronavirus Infections veterinary, Infectious bronchitis virus classification, Infectious bronchitis virus genetics, Phylogeny, Poultry Diseases virology
- Abstract
Background: The nephropathogenic avian infectious bronchitis (IB) caused unprecedented economic losses to the commercial chicken industry of China in 2008-2009. To investigate the prevalence of nephropathogenic IB in China, eighty IBV isolates from different provinces during 2008-2009 were identified by dwarf embryo test and RT-PCR., Results: The strains were mostly isolated in winter and spring with a wide age range of IB outbreaks, from 4 to 69 days. By the virus recovery trials, 70/80 of the strains resulted in the deaths or distresses of birds from nephritis. To learn more about the molecular evolutionary characteristics of the circulating field strains, the coding region of major spike 1 (S1) protein gene of these strains was RT-PCR amplified and sequenced. Compared to the published representative strains, nucleotides and amino acids sequence analysis indicated that the S1 genes of these strains and the reference strains displayed homologies ranging from 75.1% to 99.8% and from 73.1% to 99.8% respectively. S1 protein of the major pandemic strains contained 540 or 542 amino acids with the cleavage site of HRRRR or RRFRR. Phylogenetic analysis revealed that recent field isolates of IBV in China were mostly belonged to A2-branch (QXIBV-branch) and HN08-branch, only one isolate was belonged to Gray-branch and M41-branch respectively. Most of the 80 strains showed evolutionarily distant from vaccine strains., Conclusions: The results of this study suggested that nephropathogenic IBVs were mainly A2-like strains in China during 2008-2009.
- Published
- 2011
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48. Isolation and complete genomic characterization of H1N1 subtype swine influenza viruses in southern China through the 2009 pandemic.
- Author
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Liu Y, Ji J, Xie Q, Wang J, Shang H, Chen C, Chen F, Xue C, Cao Y, Ma J, and Bi Y
- Subjects
- Animals, China epidemiology, Genomics, Host Specificity, Humans, Influenza A Virus, H1N1 Subtype classification, Influenza A Virus, H1N1 Subtype physiology, Molecular Sequence Data, Orthomyxoviridae Infections virology, Phylogeny, Swine, Viral Proteins genetics, Influenza A Virus, H1N1 Subtype genetics, Influenza A Virus, H1N1 Subtype isolation & purification, Orthomyxoviridae Infections veterinary, Pandemics, Swine Diseases virology
- Abstract
Background: The swine influenza (SI) is an infectious disease of swine and human. The novel swine-origin influenza A (H1N1) that emerged from April 2009 in Mexico spread rapidly and caused a human pandemic globally. To determine whether the tremendous virus had existed in or transmitted to pigs in southern China, eight H1N1 influenza strains were identified from pigs of Guangdong province during 2008-2009., Results: Based on the homology and phylogenetic analyses of the nucleotide sequences of each gene segments, the isolates were confirmed to belong to the classical SI group, with HA, NP and NS most similar to 2009 human-like H1N1 influenza virus lineages. All of the eight strains were low pathogenic influenza viruses, had the same host range, and not sensitive to class of antiviral drugs., Conclusions: This study provides the evidence that there is no 2009 H1N1-like virus emerged in southern China, but the importance of swine influenza virus surveillance in China should be given a high priority.
- Published
- 2011
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49. The radiosensitizing effect of doranidazole on human colorectal cancer cells exposed to high doses of irradiation.
- Author
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Zhang L, Gong A, Ji J, Wu Y, Zhu X, Lv S, Lv H, and Sun X
- Subjects
- Cell Line, Tumor, Cell Proliferation drug effects, Cell Proliferation radiation effects, Gamma Rays therapeutic use, Humans, Colorectal Neoplasms drug therapy, Colorectal Neoplasms radiotherapy, Imidazoles therapeutic use, Radiation-Sensitizing Agents therapeutic use
- Abstract
Background: This paper investigates the effects of a new radiosensitizer, doranidazole, and enhancing irradiation on colorectal cancer cells., Methods: The radiosensitizing effect of doranidazole was determined using colony formation and propidium iodide (PI) assays to measure cell growth inhibition and the cell killing effect of human colorectal cancer cell lines exposed to high doses of gamma-ray irradiation under hypoxic conditions in vitro. Fluorescence staining and cell migration assays were also used to assess the radiosensitizing effect., Results: Cell proliferation evaluated by clonogenic survival curves was significantly inhibited by 5 mmol/L doranidazole, particularly at doses ranging from 10 to 30 Gy of irradiation. The radiosensitizing effect of doranidazole on colorectal cancer cells occurs in a time- and dose-dependent manner. Doranidazole also inhibited the mobility of cell invasion and migration., Conclusion: Doranidazole can enhance the killing effect and the cell growth inhibition of colorectal cancer after high-dose irradiation in a time and dose-dependent manner.
- Published
- 2007
- Full Text
- View/download PDF
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