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A recent study published in Stem Cell Week discusses the limitations of current single-cell proteomics (SCP) analyses, which primarily measure protein abundances. The researchers introduce a new method called SC-pSILAC, which combines protein abundance and turnover measurements in single cells using a stable isotope labeling technique. The study demonstrates the effectiveness of SC-pSILAC in analyzing drug effects on protein turnover and in studying the differentiation of human induced pluripotent stem cells. This comprehensive analysis provides new insights into cellular diversity and expands the scientific relevance of SCP in biological research. However, it is important to note that this study has not yet undergone peer review. [Extracted from the article]

A preprint abstract from biorxiv.org discusses the potential sex differences in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) and the importance of considering sex as a variable in in vitro studies. The study generated three female and three male hiPSC-lines to study sex differences in cardiac models. The results showed enhanced calcium handling in female-derived hiPSC-CM compared to male, and over 300 differentially expressed genes between the two sexes. The findings suggest that sex should be considered as a variable in studies related to cardiomyocyte function and cardiac health. However, it is important to note that this preprint has not been peer-reviewed. [Extracted from the article]

A recent preprint abstract discusses the development of a method to induce meiosis, an important reproductive process, from human pluripotent stem cells. The researchers used DNMT1 inhibition, retinoid signaling activation, and overexpression of certain regulatory factors to rapidly activate meiosis in the stem cells. The study demonstrated key aspects of meiosis, such as chromosome synapsis and sex body formation, and found that the meiotic cells expressed genes similar to those found in meiotic oogonia in vivo. This research provides an accessible system for inducing human meiosis in vitro, which could accelerate research and the development of therapies for infertility. [Extracted from the article]

Researchers at Texas A&M University have discovered new ways to use inorganic materials for tissue repair and regeneration. In two recently published articles, they found that inorganic ions can activate cellular pathways for bone and cartilage formation in stem cells, and mineral-based nanomaterials can aid musculoskeletal regeneration. These findings have the potential to improve treatment outcomes, reduce the need for invasive procedures and long-term medication, and provide tailored solutions for complex medical issues. The researchers are also exploring the use of inorganic biomaterials in conjunction with 3D bioprinting techniques to design custom bone implants for reconstructive injuries. [Extracted from the article]

A recent preprint abstract suggests that extracellular vesicles (EVs) derived from induced neural stem cells (iNSCs) could be an effective treatment for brain cancer. The researchers found that these EVs, called Exo-iNSC-TRAIL, were able to selectively accumulate within tumor foci and kill brain cancer cells more effectively than free TRAIL. In mouse models, Exo-iNSC-TRAIL significantly reduced tumor growth and increased survival rates. These findings indicate that Exo-iNSC-TRAIL could be a promising and easily generated treatment option for various forms of brain cancer. However, it is important to note that this research has not yet undergone peer review. [Extracted from the article]

Researchers at the German Cancer Research Center have developed a new method for generating induced pluripotent stem cells (iPSCs) using nonviral SMAR DNA vectors. iPSCs are important for biomedical research, but their production has safety concerns when using viruses or virus-based methods. The study shows that iPSCs can be generated using nonviral plasmids without the use of potentially oncogenic systems, and the resulting iPSCs have comparable pluripotency and cellular function to those generated with viruses. This research provides a promising alternative for the production of iPSCs with fewer safety concerns. [Extracted from the article]

A recent study conducted by researchers at the School of Stomatology in Shandong, China, explores the role of autophagy in the differentiation of mesenchymal stem cells (MSCs) and its potential impact on regenerative medicine. Autophagy is a cellular process that helps maintain cellular homeostasis by eliminating harmful substances. The researchers highlight the need for a deeper understanding of how autophagy functions and its influence on MSC differentiation. This study provides valuable insights into the potential therapeutic applications of MSCs in treating various diseases. [Extracted from the article]

According to a preprint abstract, researchers have discovered that aneuploid human pluripotent stem cells (hPSCs), which have chromosome imbalances, are eliminated through cell competition with neighboring diploid hPSCs. Aneuploid hPSCs with lower Myc or higher p53 levels are outcompeted, but gain a selective advantage when the relative abundance of Myc and p53 switches. These findings have implications for the use of hPSCs in regenerative medicine and the establishment of diploid human embryos. The preprint has not yet been peer-reviewed. [Extracted from the article]

A recent study from Tabriz University of Medical Sciences in Iran discusses the pros and cons of stem cell-based therapies and stem cell-free therapies. The research highlights the therapeutic effects of stem cells and their ability to release extracellular vesicles containing cytokines and bioactive factors. However, the study also acknowledges the limitations of transplanted stem cells in terms of stability and durability at the site of injury. As an alternative, the study suggests using exosomes, which have fewer side effects. The effectiveness and limitations of both whole stem cell-based therapies and stem cell-free therapies are discussed in the clinical setting. [Extracted from the article]

A recent study from Ancona, Italy, discusses the challenges in treating FLT3mut AML, a type of leukemia. Despite the availability of target drugs, only 30% of patients with FLT3mut AML are cured. The study highlights the difficulty in eradicating FLT3mut leukemic stem cells (LSC) due to their metabolic and genomic characteristics. The researchers propose potential therapeutic targets, such as reactivation of glycogen synthesis, inhibition of the RAS/MAPK pathway, and degradation of FLT3. They also explore new treatment options, including chimeric antigen receptor T cells (CAR-T), bispecific T-cell engager molecules (BiTEs), CAR-NK, and nanoparticles. The combination of these therapies, along with a minimal residual disease (MRD)-driven approach, could provide a promising solution for treating FLT3mut AML. [Extracted from the article]

Researchers from Comenius University in Bratislava have conducted a study comparing different methods of extracting metabolites from human mesenchymal stem cells (MSCs) and fibroblasts. The study aimed to determine the most efficient method for extracting metabolites from these cells, which are commonly used in regenerative medicine. The researchers found that direct scraping to organic solvent yielded higher abundances of metabolites, and extraction with polar reagents such as methanol, acetonitrile, and methanol-chloroform showed the same quality. This study provides a protocol for maximizing extraction yield and may be useful for large-scale comparative metabolomic studies of human MSC samples. [Extracted from the article]

According to a preprint abstract, researchers have discovered that disrupting epigenetic barriers in olfactory placode-derived cells can transform them into cells with stem cell characteristics. This transformation was dependent on the expression of the large T-antigen and was enhanced by Sox2 over-expression. These findings suggest that there is inherent cellular plasticity within early cell types of the olfactory lineage and may contribute to our understanding of cellular reprogramming. However, it is important to note that this preprint has not yet undergone peer review. [Extracted from the article]

A study conducted in Uttar Pradesh, India, has found that exposure to bisphenol-F (BPF) and bisphenol-S (BPS), which are alternatives to bisphenol-A (BPA), can impair the stemness of neural stem cells (NSCs) and affect neuronal fate decision in the hippocampus. The research showed that exposure to BPF and BPS decreased the number and size of neurospheres, as well as markers for NSC proliferation and neuronal differentiation. Additionally, BPF and BPS treatment led to increased apoptosis in NSCs, myelin degeneration, synapse degeneration, and impaired learning and memory in rats. This study provides insights into the neurotoxic effects of BPF and BPS on NSCs and highlights the potential risks associated with their usage. [Extracted from the article]

A preprint abstract from biorxiv.org discusses the potential use of bacterial protein polymers to support the growth and differentiation of induced pluripotent stem cells (iPSCs). The study focuses on the Caf1 protein of Yersinia pestis, which is known for its stability, modularity, and ease of production. The researchers have developed novel variants of Caf1 that perform as well as commonly used attachment substrates like GelTrex. This research suggests that Caf1 laminin and vitronectin mimics could be viable alternatives for supporting iPSC growth and differentiation, offering more cost-effective and readily available attachment substrates in iPSC research and applications. However, it is important to note that this preprint has not been peer-reviewed. [Extracted from the article]

A recent study conducted by the Research Center for Medical Genetics in Moscow, Russia, has found that human-induced airway basal cells (hiBCs) derived from human-induced pluripotent stem cells (hiPSCs) show promise as a cell model for studying lung diseases, regenerative medicine, and developing new gene therapy methods. The researchers developed a protocol for obtaining hiBCs and demonstrated that these cells can differentiate into lung organoids (LOs) and assess cystic fibrosis transmembrane conductance regulator (CFTR) channel function. They also tested different transgene delivery methods and found that recombinant adeno-associated virus (rAAV) serotype 6 was the most effective for gene therapy research. This research provides new insights into cystic fibrosis research and potential treatments. [Extracted from the article]

This article discusses the mechanisms behind resistance to KRAS-targeted therapy in colorectal cancer (CRC). The study found that cancer-associated fibroblasts (CAFs) play a role in inducing therapy resistance by generating and maintaining cancer stem cells (CSCs). Additionally, CAFs secrete factors that can modulate the proteomic profile of KRAS mutant CRC cells, leading to increased stemness and resistance to KRAS inhibition. The findings suggest that the limited success of KRAS-targeted therapies may be influenced by external factors from the tumor microenvironment, highlighting the need to consider these factors in improving therapy responses in CRC. [Extracted from the article]

Purdue University researchers are developing a patent-pending treatment for incurable glioblastoma brain tumors. Traditional methods like chemotherapy and immunotherapy are often ineffective against glioblastoma. The researchers are developing a novel immunotherapy using genetically engineered immune cells sourced from induced pluripotent stem cells. Animal studies have shown promising results, and the next step is to conduct clinical trials. The researchers are seeking funding and collaborations to bring this therapy to patients with brain tumors. [Extracted from the article]

A recent preprint abstract discusses the findings of a study on autism spectrum disorder (ASD) using human stem cell models. The study aimed to identify shared and distinct mutational mechanisms in ASD by analyzing a large cohort of hiPS cell lines. The researchers found convergent patterns of dysregulation across ASD post-mortem brain tissue and identified a shared RNA and protein interaction network enriched in ASD risk genes. These findings provide new insight into the impact of genetic risk on human neurodevelopment in ASD. However, it is important to note that this preprint has not yet undergone peer review. [Extracted from the article]

Researchers from Edge University in Izmir, Turkey have conducted a study on the effects of a synthetic cannabinoid called JWH-018 on leukemia cells and leukemic stem cells (LSCs). The study found that JWH-018 exhibited antileukemic effects and could potentially be used as a therapeutic approach for leukemia. However, further research is needed to fully understand the effects of synthetic cannabinoids on leukemogenesis. This research has been peer-reviewed and published in the journal Cannabis and Cannabinoid Research. [Extracted from the article]

A recent report from the Steadman Philippon Research Institute in Vail, Colorado discusses the challenges of treating segmental bone defects caused by trauma, infection, tumor resection, or osteoporotic fractures. The report highlights the need for new bone tissue engineering strategies and summarizes the progress made in using different types of stem cells for bone tissue engineering and repair. The advantages and disadvantages of each type of stem cell are discussed, as well as the use of exosomes or extracellular vesicles for bone repair. The researchers hope that these preclinical results will lead to new regenerative therapies for bone defect repair in the future. [Extracted from the article]

A recent study conducted by researchers at Southern Medical University in Guangzhou, China, explores the therapeutic potential of mesenchymal stem cells (MSCs) for cerebral small vessel disease (CSVD). CSVD is a common and progressive vascular disease that affects the brain and is a leading cause of stroke and vascular cognitive impairment. The current treatment for CSVD focuses on managing vascular risk factors, but controlling these factors alone does not halt the progression of brain injury. MSC therapy has emerged as a promising treatment modality for central nervous system diseases due to their paracrine properties and immunoregulatory effects. The study aims to provide clinicians and researchers with a better understanding of recent progress and future directions in the field of MSC therapy for CSVD. [Extracted from the article]

Pelage Pharmaceuticals, a regenerative medicine company, has secured $16.75 million in Series A financing to develop a treatment for hair loss. The company has completed a Phase 1 clinical trial for a topical agent called PP405, which showed promising results in activating hair follicle stem cells. The treatment has the potential to address androgenetic alopecia (pattern baldness), as well as other forms of hair loss. Pelage Pharmaceuticals aims to advance to a Phase 2a clinical trial later this year. [Extracted from the article]

A preprint abstract from biorxiv.org discusses the evaluation of melanocyte stem cell population in vitiliginous skin before and after narrow-band UVB phototherapy. Vitiligo vulgaris is an autoimmune disorder that causes the destruction of epidermal melanocytes, resulting in white patches on the skin. The study found that narrow-band UVB phototherapy promotes the migration of melanocyte stem cells to the epidermis, leading to repigmentation in affected individuals. Additionally, the therapy was effective in reducing overall immune load, which is considered a major contributor to the disease. However, it is important to note that this preprint has not been peer-reviewed. [Extracted from the article]

This article discusses a study on the regeneration process in Arabidopsis thaliana (Arabidopsis), a plant species known for its remarkable regenerative abilities. The researchers aimed to understand the role of cell division and chromatin remodeling in this process. They found that while cell cycle inhibition blocks regeneration, some partial reprogramming can still occur. The study also revealed that the cell cycle is accelerated during root regeneration and that histone deacetylase (HDAC) activity is critical in the early stages of regeneration. The researchers propose that Class I HDACs, particularly HDA19, play a key role in early reprogramming during root tip regeneration. [Extracted from the article]

A recent preprint abstract discusses the development of an atrial-specific in-silico model for stem cell-derived cardiomyocytes. This model aims to improve our understanding of cardiac electrophysiology and its potential applications in personalized medicine, particularly in the treatment of conditions like atrial fibrillation. By leveraging experimental data and using automatic optimization techniques, the model was able to accurately simulate action potential shape and validate against rate dependence and ion current blocking data. This research offers new tools for drug evaluation, therapeutic improvement, and a deeper comprehension of cardiac phenotypes. However, it is important to note that this preprint has not yet undergone peer review. [Extracted from the article]

A study conducted by researchers at the University of Michigan explores the limitations of studying morphogenetic events during development due to technical and ethical constraints. The researchers discuss the use of stem cell-based in vitro models and theoretical models as alternatives to studying natural development. These models offer accessibility, efficiency, and modulability, but their physiological relevance is often unclear. The study highlights the accomplishments, challenges, and opportunities of these models in advancing our understanding of mammalian development, including human development. [Extracted from the article]

A recent report from the University of Michigan discusses the advances in embryonic stem cells, specifically focusing on pituitary stem cells. These cells have the ability to self-renew and differentiate into multiple pituitary hormone-producing cell types. However, the ability to mobilize these stem cells decreases with age. Pituitary organoids, which can be formed from embryonic stem cells or induced pluripotent stem cells, have the potential for therapeutic applications and can be used to study hormone production and genetic variants. The research also highlights the need for further understanding of the regulation of stem cell quiescence and activation, as well as the roles of stem cells in pituitary tumors. [Extracted from the article]

Scientists have successfully grown "mini-placentas" in the lab, allowing them to study the development and interaction of the placenta with the lining of the womb. This research could provide insights into the causes of pre-eclampsia and other pregnancy disorders. The study, published in Cell Stem Cell, demonstrates that it is possible to experiment on a developing human placenta rather than just observing specimens. By using these "mini-placentas," researchers were able to identify proteins and genes that play a crucial role in successful implantation and blood flow, offering potential avenues for future research on pre-eclampsia. [Extracted from the article]

A preprint abstract from biorxiv.org discusses the potential effects of nicotine on intestinal stem cells (ISCs). The study found that nicotine increases the abundance and proliferative activity of ISCs in vivo and ex vivo. It also activates the Yes-associated protein (YAP)/Transcriptional coactivator with PDZ-binding motif (TAZ) and Notch signaling pathways in ISCs through the a7-nicotinic acetylcholine receptor (nAchR) and protein kinase C (PKC) activation. The study suggests that inhibiting Notch signaling with dibenzazepine (DBZ) could potentially be a therapeutic strategy for treating intestinal tumors. However, it is important to note that this preprint has not been peer-reviewed. [Extracted from the article]

A study conducted in Bucharest, Romania, examined the impact of stem cell transplants on women's sexual dysfunctions and their impact on couple relationships. The study included 38 patients who underwent stem cell transplants and 38 healthy patients as a control group. The results showed that the transplanted women experienced vulvovaginal atrophy and sexual dysfunctions, which had a negative impact on their relationships. The study highlights the importance of addressing and assessing these difficulties to improve the quality of life and relationships of long-term survivors after stem cell transplants. [Extracted from the article]

A preprint abstract from biorxiv.org discusses the directed differentiation of pluripotent stem cells into adrenocortical cells. The researchers used single-cell transcriptomic data to develop an in vitro differentiation system from mouse embryonic stem cells. They found that culturing cells on fibronectin during differentiation increased the expression of the steroidogenic marker NR5A1. Additionally, 3D cultures in the presence of PKA-pathway activators led to the formation of aggregates composed of different cell types expressing adrenal progenitor or steroidogenic markers. The in vitro differentiated cells secreted the zona Fasciculata specific corticosterone in response to cAMP activators, confirming the specificity of differentiation towards the adrenal lineage. However, it is important to note that this preprint has not been peer-reviewed. [Extracted from the article]

A recent article from Stem Cell Week discusses new research on mesenchymal stem cells conducted by Korea University. The researchers highlight the importance of stem cell tracking in understanding their mechanisms of action and ensuring safe and effective treatments. They emphasize the advantages of fluorescence-based imaging for stem cell tracking, including high resolution, real-time monitoring, and multi-fluorescence detection. The article also explores various labeling strategies for stem cells, such as green fluorescence protein tagging and nanoparticle uptake. The research concludes by presenting several combinations of strategies for efficient and precise detection. For more information, readers can refer to the original article published in Biomolecules. [Extracted from the article]

A recent article from Stem Cell Week discusses the challenges of visualizing and tracking stem cells in vivo for clinical applications. The article highlights the limitations of current imaging methods, such as optical imaging, magnetic resonance imaging (MRI), and nuclear medicine imaging. These methods either lack tissue penetration, have low cellular resolution, or involve radioactive materials. The researchers propose multimodal imaging as a solution, which combines the advantages of different imaging modalities to track stem cells in a multidimensional way. The article provides an overview of various multimodal imaging technologies and labeling methods, comparing their advantages and disadvantages, and discussing the prospects and challenges of human stem cell tracking techniques. [Extracted from the article]

New findings in ovarian cancer were discussed by investigators from the Medical University in Lublin, Poland. Ovarian cancer is a significant health concern, with a high mortality rate and challenges in early diagnosis. The presence of cancer stem cells (CSCs) has been linked to tumor recurrence and resistance to chemotherapy. This review focuses on the expression of specific CSC markers that show potential prognostic significance, although the determination of specific CSC phenotypes remains difficult due to their heterogeneity and plasticity. Further research is needed to develop reliable diagnostic and prognostic biomarkers for ovarian cancer. [Extracted from the article]

A preprint abstract from biorxiv.org discusses the role of quiescence and its associated molecular mechanisms in acute myeloid leukemia (AML) and leukemic stem cells (LSCs). The study found that a subset of quiescent cells in AML patients exhibited a distinct gene set of prognostic significance. These cells also displayed heightened autophagic activity, specifically relying on ferritinophagy, a form of autophagy mediated by Nuclear Receptor Coactivator 4 (NCOA4) that regulates iron bioavailability. Inhibition of NCOA4 showed potent anti-leukemic effects, particularly targeting the LSC compartment. The study suggests that ferritinophagy inhibition may be a promising therapeutic strategy for AML patients. [Extracted from the article]

A preprint abstract from biorxiv.org discusses the use of a mathematical model to study hematopoiesis, the process of blood cell formation. The researchers parameterized the model using mouse data and included homeostatic control mechanisms and clonal evolution. They found that non-linear feedback control affects the interpretation of kinetic estimates at homeostasis and that short-term hematopoietic stem cells and multipotent progenitors can dynamically adjust to sustain themselves even in the absence of long-term stem cells. The presence of feedback control in the model also makes the system resilient against mutant invasion, although age-related changes in stem cell differentiation and a mutant-associated inflammatory environment can overcome invasion barriers. This research provides insights into the evolution of certain mutants and potential strategies to reduce mutant burden. [Extracted from the article]

A recent report from the Department of Pharmacology and Toxicology highlights the role of stem cell therapy in the treatment of cancer. Stem cell therapy shows promise in addressing the challenges of cancer treatment, including immune rejection and disease relapse. The use of cancer stem cells as a therapeutic source also holds potential for early cancer diagnosis. While there are challenges to overcome, continued advancements in stem cell therapy offer hope and innovation in the fight against cancer. [Extracted from the article]

A recent preprint abstract discusses the impact of genetically variant human pluripotent stem cells on cardiomyocyte differentiation and phenotype. The study found that the presence of recurrent gains of chromosome 1q in these stem cells affects the efficiency of differentiation and the characteristics of the resulting cardiomyocytes. The researchers identified aberrant activation of the Wnt signaling pathway as the underlying mechanism for this skewed differentiation. These findings have implications for the production of cardiomyocytes for research and clinical applications, highlighting the need to address genetically variant cells in cultures. However, it is important to note that this preprint has not yet undergone peer review. [Extracted from the article]

A recent study on Alzheimer's disease highlights the neurodegenerative nature of the disorder, particularly affecting the hippocampus region of the brain. The cognitive decline and memory loss associated with Alzheimer's are major characteristics of the disease. While there is currently no definitive cure or medication to halt the progression of neurodegeneration, progress has been made in addressing the symptoms. Stem cell therapy, specifically the use of mesenchymal stem cells, is being investigated as a potential treatment option for Alzheimer's disease. [Extracted from the article]

A report from Urmia University discusses the use of exosomes derived from mesenchymal stem cells (MSCs) as carriers for nanoparticles in cancer therapy. The researchers highlight the unique properties of MSCs-Exo in delivering therapeutic agents to tumor cells. They also discuss the incorporation of different nanoparticles, such as gold nanoparticles, into MSCs-Exo for improved anticancer effects. However, the use of nanoparticle-loaded MSCs-Exo as a drug-delivery tool still faces challenges and requires further investigation before translation to clinical applications. The study provides valuable insights into the potential of engineered MSCs-Exo for targeted cancer therapy. [Extracted from the article]

A recent study published in the journal Neuron by scientists at the Institute of Science and Technology Austria (ISTA) has provided new insights into the development of the superior colliculus in the mammalian brain. The researchers mapped the lineage of neural stem cells in this brain region and discovered that the development of the superior colliculus differs from other brain regions. They also found that a critical gene called Pten plays a role in establishing the appropriate balance of cell types in the superior colliculus, and its disruption may contribute to neurodevelopmental disorders such as autism and ADHD. This research sheds light on the complex processes involved in brain development and may have implications for understanding and treating neurological disorders. [Extracted from the article]

A recent preprint abstract discusses a novel model for studying human lung regeneration using human pluripotent stem cells. The researchers developed expandable cell lines that resemble airway secretory and basal cells, which were then engrafted into the lungs of rats after a conditioning process. These engrafted cells, called distal lung epithelial progenitors (DLEPs), contributed to the repair of alveolar epithelial cells and generated structures involved in lung repair. The study suggests that this model could help further our understanding of lung regeneration and potentially lead to the development of cell therapies for lung injury and disease. However, it is important to note that this research has not yet undergone peer review. [Extracted from the article]

A new study conducted by researchers at the Joint Shantou International Eye Center in Guangdong, China, explores the transdifferentiation potential of human adipose-derived stem cells (ASCs) into retinal lineage. The study found that continuous non-adherent culture and NDI induction medium both independently promote the transdifferentiation of ASCs into retinal lineage. However, the combination of the two did not produce an enhancement effect. This research provides valuable insights into the potential applications of stem cells in retinal regeneration and further understanding of the transdifferentiation process. [Extracted from the article]

A recent report from the National and Kapodistrian University of Athens discusses the role of cancer stem cells (CSCs) in tumor development and resistance to treatment. The study highlights the metabolic characteristics of CSCs and their similarities and differences to normal stem cells. The researchers emphasize the potential of targeting CSC metabolism as a strategy for more effective clinical responses in the future. This information may be valuable for individuals researching cancer, oncology, stem cell research, and potential therapies. [Extracted from the article]

A recent report from the Department of Tissue Engineering discusses the treatment of premature ovarian insufficiency (POI) using stem cell therapy and exosome therapy. The report highlights the features, limitations, and challenges of both therapies, providing insights for comparing and selecting chemical agents and optimizing doses. While stem cell therapy has shown some improvement in ovarian function in animals with POI, it has drawbacks such as high cost, limited cell sources, and potential side effects. Exosome therapy, a newer therapy, shows promise but requires further research to determine its effectiveness and safety. The study offers a new perspective for researchers in the field of cell-based and cell-free therapies. [Extracted from the article]

This article discusses a preprint abstract that focuses on the differentiation of pluripotent stem cells into various cardiac cell types. The researchers developed a guided differentiation protocol that allows for the rapid generation of diverse cardiac-relevant cell types. This protocol enables the characterization of regulatory variation and gene by environment interactions in spatially and temporally diverse cardiac cell types. The article emphasizes the potential of induced pluripotent stem cells (iPSCs) combined with single-cell RNA sequencing for mapping regulatory quantitative trait loci (QTLs) in different contexts. However, it is important to note that this preprint has not yet undergone peer review. [Extracted from the article]

A preprint abstract from biorxiv.org discusses a study on the metabolic pathways of hematopoietic stem cells (HSCs) in response to stress. The researchers aimed to identify the key metabolic enzymes that contribute to glycolytic metabolism differences between steady-state and stress conditions in HSCs. Through their analysis, they found that HSCs activate the enzyme phosphofructokinase (PFK) during proliferation and oxidative phosphorylation (OXPHOS) inhibition, leading to accelerated glycolysis to meet ATP requirements. The study also revealed the regulation of PFKFB3, an allosteric PFK activator, through methylation and phosphorylation. This research provides insights into the flexible regulation of HSC metabolism under stress and offers techniques to better understand the metabolism of rare hematopoietic cells. [Extracted from the article]

This article discusses the creation of a human endoderm organoid cell atlas (HEOCA) through the integration of single-cell transcriptomes from various organoids derived from endodermal tissues. The atlas includes data from diverse conditions, sources, and protocols, covering tissues such as lung, pancreas, intestine, liver, biliary system, stomach, and prostate. The researchers align and compare cell types and states between organoid models and map the atlas to primary tissue counterparts. The HEOCA can be used to assess organoid fidelity, characterize perturbed and diseased states, and streamline protocol development. However, it is important to note that this preprint has not been peer-reviewed. [Extracted from the article]

A recent report from investigators at the University of California San Diego (UCSD) discusses the use of biophysical cues and biomaterials to improve genetic models in personalized medicine. The researchers highlight the advancements made possible by induced pluripotent stem cells and modern differentiation protocols in understanding diseases through in vitro disease modeling. They suggest that combining biomaterials and advanced microphysiological systems with stem cells expressing disease-mimicking genetics could enhance disease modeling efforts. The report concludes by discussing the current challenges and future potential of creating more personalized therapeutics. This research has been peer-reviewed and is available in the journal Current Opinion in Biomedical Engineering. [Extracted from the article]