43 results on '"Ma, Xiaoming"'
Search Results
2. Identification of circRNA-associated ceRNA networks in the longissimus dorsi of yak under different feeding systems.
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Ma, Xiaoming, Guo, Xian, Yongfu, La, Wang, Tong, Bao, Pengjia, Chu, Min, Wu, Xiaoyun, Yan, Ping, and Liang, Chunnian
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COMPETITIVE endogenous RNA , *YAK , *ERECTOR spinae muscles , *MUSCLE growth , *MEAT quality , *SHEARING force , *ANIMAL species , *SKELETAL muscle , *HUMAN fingerprints - Abstract
Background: Yaks (Bos grunniens), prized for their ability to thrive in high-altitude environments, are indispensable livestock in the plateau region. Modifying their feeding systems holds significant promise for improving their growth and meat quality. Tenderness, a key determinant of yak meat quality and consumer appeal, is demonstrably influenced by dietary regimen. Indoor feeding regimes have been shown to enhance tenderness by lowering shear stress and optimizing pH values. CircRNAs, well-known modulators of circulatory function, also play a crucial role in skeletal muscle development across various animal species. However, their functional significance in yak skeletal muscle remains largely unexplored. Results: In this study, we identified a total of 5,534 circRNAs within the longissimus dorsi muscle, and we found 51 differentially expressed circRNAs (20 up-regulated and 31 down-regulated) between the two feeding groups. Constructing a comprehensive ceRNA network illuminated intricate regulatory mechanisms, with PGP and circRNA_0617 converging on bta-miR-2285q, mirrored by KLF15/circRNA_0345/bta-miR-20b and CTSF/circRNA_0348/bta-miR-146a. These findings shed light on the potential of circRNAs to influence yak muscle development and meat quality, offering valuable insights for future research. Conclusions: This investigation unraveled a complex interaction network between circRNAs、mRNAs and miRNAs in yak skeletal muscle. We further elucidated the target genes regulated by these target genes within the network, offering valuable insights into the potential regulatory mechanisms governing muscle development and meat quality-related traits in yaks. [ABSTRACT FROM AUTHOR]
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- 2024
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3. A comprehensive study on the longissius dorsi muscle of Ashdan yaks under different feeding regimes based on transcriptomic and metabolomic analyses.
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Wang, Tong, Ma, Xiaoming, Zheng, Qingbo, Ma, Chaofan, Zhang, Zhilong, Pan, Heping, Guo, Xian, Wu, Xiaoyun, Chu, Min, Liang, Chunnian, and Yan, Ping
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AbstractYak is an important dominant livestock species at high altitude, and the growth performance of yak has obvious differences under different feeding methods. This experiment was conducted to compare the effects of different feeding practices on growth performance and meat quality of yaks through combined transcriptomic and metabolomic analyses. In terms of yak growth performance, compared with traditional grazing, in-house feeding can significantly improve the average daily weight gain, carcass weight and net meat weight of yaks; in terms of yak meat quality, in-house feeding can effectively improve the quality of yak meat. A combined transcriptomic and metabolomic analysis revealed 31 co-enriched pathways, among which arginine metabolism, proline metabolism and glycerophospholipid metabolism may be involved in the development of the longissimus dorsi muscle of yak and the regulation of meat quality-related traits. The experimental results increased our understanding of yak meat quality and provided data materials for subsequent deep excavation of the mechanism of yak meat quality. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Remote Radical 1,3-, 1,4-, 1,5-, 1,6- and 1,7-Difunctionalization Reactions †.
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Ma, Xiaoming, Zhang, Qiang, and Zhang, Wei
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RADICALS (Chemistry) , *ABSTRACTION reactions , *FUNCTIONAL groups , *ELECTROPHILES , *ORGANIC synthesis , *RADICALS - Abstract
Radical transformations are powerful in organic synthesis for the construction of molecular scaffolds and introduction of functional groups. In radical difunctionalization reactions, the radicals in the first functionalized intermediates can be relocated through resonance, hydrogen atom or group transfer, and ring opening. The resulting radical intermediates can undertake the following paths for the second functionalization: (1) couple with other radical groups, (2) oxidize to cations and then react with nucleophiles, (3) reduce to anions and then react with electrophiles, (4) couple with metal-complexes. The rearrangements of radicals provide the opportunity for the synthesis of 1,3-, 1,4-, 1,5-, 1,6-, and 1,7-difunctionalization products. Multiple ways to initiate the radical reaction coupling with intermediate radical rearrangements make the radical reactions good for difunctionalization at the remote positions. These reactions offer the advantages of synthetic efficiency, operation simplicity, and product diversity. [ABSTRACT FROM AUTHOR]
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- 2023
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5. Genome-Wide Landscape of mRNAs, lncRNAs, and circRNAs during Testicular Development of Yak.
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La, Yongfu, Ma, Xiaoming, Bao, Pengjia, Chu, Min, Yan, Ping, Liang, Chunnian, and Guo, Xian
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YAK , *LINCRNA , *GENE expression , *CELL differentiation , *MESODERM , *CIRCULAR RNA - Abstract
Testicular development is a tightly regulated process in mammals. Understanding the molecular mechanisms of yak testicular development will benefit the yak breeding industry. However, the roles of different RNAs, such as mRNA, lncRNA, and circRNA in the testicular development of yak, are still largely unclear. In this study, transcriptome analyses were performed on the expression profiles of mRNAs, lncRNAs, and circRNAs in testis tissues of Ashidan yak at different developmental stages, including 6-months-old (M6), 18-months-old (M18), and 30-months-old (M30). A total of 30, 23, and 277 common differentially expressed (DE) mRNAs, lncRNAs, and circRNAs were identified in M6, M18, and M30, respectively. Furthermore, functional enrichment analysis showed that the common DE mRNAs during the entire developmental process were mainly involved in gonadal mesoderm development, cell differentiation, and spermatogenesis processes. Additionally, co-expression network analysis identified the potential lncRNAs related to spermatogenesis, e.g., TCONS_00087394 and TCONS_00012202. Our study provides new information about changes in RNA expression during yak testicular development, which greatly improves our understanding of the molecular mechanisms regulating testicular development in yaks. [ABSTRACT FROM AUTHOR]
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- 2023
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6. Visible light-driven selective oxidation of amines by cooperative photocatalysis of niobium oxide nanorods with an electron–proton transfer mediator.
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Ma, Xiaoming, Li, Xia, Wang, Yuexin, and Lang, Xianjun
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NIOBIUM oxide , *PHOTOCATALYSIS , *NANORODS , *OXIDATION-reduction reaction , *AMINES , *PROTON transfer reactions , *REACTIVE oxygen species , *ALCOHOL oxidation - Abstract
[Display omitted] • Nb 2 O 5 nanorods are synthesized with a specific surface area of 166 m2/g. • The dissociative adsorption of amine on Nb 2 O 5 affords a surface complex. • The surface complex can facilitate selective oxidation of amine to imine. • TEMPO+PF 6 − acts as an electron–proton transfer mediator. • Superoxide is the dominant reactive oxygen species. Cooperative photocatalysis has gained increasing interest to better an existing redox reaction with a mediator. Herein, an ingenious system is constructed for the visible light-driven selective oxidation of a variety of amines by merging niobium oxide (Nb 2 O 5) photocatalysis with an electron–proton transfer mediator, 2,2,6,6-tetramethylpiperidine-1-oxoammonium hexafluorophosphate (TEMPO+PF 6 −). The visible light-driven dissociative adsorption of amine over Nb 2 O 5 nanorods gives rise to an amine–Nb 2 O 5 complex with a weak response to visible light (λ < 460 nm). With adding 4 mol% of TEMPO+PF 6 −, the conversion of benzylamine over Nb 2 O 5 is approximately 2.5 times that without TEMPO+PF 6 −. The proton and electron transfer between the amine–Nb 2 O 5 complex and TEMPO+PF 6 − is the key to assuring cooperative photocatalysis. Besides, the oxidant dioxygen is predominantly reduced to superoxide anion in affording the imine products. This work highlights it is feasible to ameliorate selective organic conversions over metal oxide photocatalysts with a suited mediator. [ABSTRACT FROM AUTHOR]
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- 2023
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7. Identification and profiling of microRNAs during yak's testicular development.
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La, Yongfu, Ma, Xiaoming, Bao, Pengjia, Chu, Min, Guo, Xian, Liang, Chunnian, and Yan, Ping
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YAK , *SPERMATOGENESIS , *NON-coding RNA , *GENE expression , *MICRORNA , *TESTIS development - Abstract
Background: Normal testicular development is highly crucial for male reproduction and is a precondition for spermatogenesis that is the production of spermatozoa in the testes. MiRNAs have been implicated in several testicular biological processes, including cell proliferation, spermatogenesis, hormone secretion, metabolism and reproductive regulation. In the present study, we used deep sequencing data to study the functions of miRNAs during testicular development and spermatogenesis, by analyzing the expression patterns of small RNAs in 6-, 18- and 30-month-old yak testis tissues. Results: A total of 737 known and 359 novel miRNAs were obtained from 6-, 18- and 30-month-old yak testes. In all, we obtained 12, 142 and 139 differentially expressed (DE) miRNAs in 30- vs. 18-, 18- vs. 6-, and 30- vs. 6-month-old testes, respectively. Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of all DE miRNA target genes revealed BMP2, TGFB2, GDF6, SMAD6, TGFBR2 and other target genes as participants in different biological processes, including TGF-β, GnRH, Wnt, PI3K–Akt, MAPK signaling pathways and several other reproductive pathways. In addition, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was used to detect the expression of seven randomly selected miRNAs in 6-, 18- and 30-month-old testes, and the results were consistent with the sequencing data. Conclusions: The differential expression of miRNAs in yak testes at different development stages was characterized and investigated using deep sequencing technology. We believe that the results will contribute to further understanding the functions of miRNAs in regulating the development of yak testes and improving the reproductive performance of male yaks. [ABSTRACT FROM AUTHOR]
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- 2023
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8. Identification and Characterization of Piwi-Interacting RNAs for Early Testicular Development in Yak.
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La, Yongfu, Ma, Xiaoming, Bao, Pengjia, Chu, Min, Yan, Ping, Guo, Xian, and Liang, Chunnian
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YAK , *SPERMATOGENESIS , *NON-coding RNA , *RNA sequencing , *GERM cells , *RNA - Abstract
Normal testicular development plays a crucial role in male reproduction and is the precondition for spermatogenesis. PIWI-interacting RNAs (piRNAs) are novel noncoding RNAs expressed in animal germ cells that form complexes with PIWI family proteins and are involved in germ cell development, differentiation, and spermatogenesis. However, changes in piRNA expression profiles during early testicular development in yak have not been investigated. In this study, we used small RNA sequencing to evaluate the differences and potential functions of piRNA expression profiles in 6-, 18-, and 30-month-old yak testis tissues. Differential expression analysis found 109, 293, and 336 differentially expressed piRNAs in M30 vs. M18, M18 vs. M6, and M30 vs. M6, respectively, and found 30 common differentially expressed piRNAs in the three groups of M6, M18, and M30. In addition, the functional enrichment analysis of differentially expressed piRNAs target genes indicated that they were related to testicular development and spermatogenesis. Finally, we detected the expression of the PIWI protein family in the yak testis at different developmental stages and found that PIWIL1, PIWIL2, PIWIL3, and PIWIL4 were highly expressed in 18- and 30-month-old yak testis and almost not expressed in 6-month-old yak testis. In conclusion, this study summarizes the changes of piRNA expression patterns during the early development of yak testis and provides new clues for the regulatory role of piRNA in yak testis. [ABSTRACT FROM AUTHOR]
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- 2022
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9. Study on the Seismic Performance of Stiffened Corrugated Steel Plate Shear Walls with Atmospheric Corrosion.
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Ma, Xiaoming, Hu, Yi, Jiang, Liqiang, Jiang, Lizhong, Nie, Guibo, and Zheng, Hong
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SHEAR walls , *CYCLIC loads , *IRON & steel plates , *ENERGY dissipation , *CORROSION & anti-corrosives , *PERFORMANCE theory - Abstract
Corrugated steel plate shear walls (CSPWs) with three different stiffening methods are proposed in this paper, including unstiffened CSPWs (USWs), cross stiffened CSPWs (CSWs) and asymmetric diagonal-stiffened CSPWs (ASWs). A numerical model was established by ABAQUS 6.13 based on the validation of an existing cyclic test on a CSPW. This paper presents an investigation of the lateral performance under monotonic loading, seismic performance under cyclic loading and seismic performance under atmospheric corrosion of USW, CSW and ASW. The results show that (1) Stiffeners can improve the elastic critical buckling load, the initial stiffness and the ultimate shear resistance of CSPWs, and the effect of asymmetric diagonal stiffeners is more significant than that of cross stiffeners; (2) Stiffeners can improve the energy dissipation capacity and ductility, delay stiffness degradation and reduce the out-of-plane deformation of CSPWs, and the hysteretic performance of ASWs is obviously better than that of CSWs; and (3) Under atmospheric corrosion, stiffeners are conducive to inhibiting buckling and improving the seismic performance of CSPWs, while the seismic performance of CSWs is significantly affected by corrosion, so asymmetric diagonal stiffeners are better than cross stiffeners in improving the seismic performance of CSPWs. Meanwhile, the formula of ultimate shear resistance of corroded specimens is also fitted in this paper, which can provide design suggestions for practical engineering. [ABSTRACT FROM AUTHOR]
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- 2022
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10. Blue light-powered hydroxynaphthoic acid-titanium dioxide photocatalysis for the selective aerobic oxidation of amines.
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Li, Xia, Ma, Xiaoming, and Lang, Xianjun
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METAL oxide semiconductors , *PHOTOCATALYSIS , *OXIDATION , *ATMOSPHERIC oxygen , *AMINES , *SALICYLIC acid - Abstract
Cooperative photocatalysis of 2H1NA-TiO 2 with TEMPO enables the highly selective aerobic oxidation of benzylamines. [Display omitted] Solar photocatalysis is the key to resolve many environmental challenges but is usually hard to achieve over a metal oxide semiconductor. Therefore, assembling π-conjugated molecules onto semiconductors becomes an efficient approach to solar conversion via ligand-to-metal charge transfer. Here, a rational design of ligands for titanium dioxide (TiO 2) is presented to produce robust visible light photocatalysts. Three hydroxynaphthoic acids (HNAs) were selected as ligands by extending an extra benzene ring of salicylic acid (SA) at 3,4 or 4,5 or 5,6 positions. These ligands could regulate the performance of TiO 2 in which 2-hydroxy-1-naphthoic acid (2H1NA) endows the best outcome. In detail, blue light-powered cooperative photocatalysis of 2H1NA-TiO 2 with 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO, 5 mol%) inaugurates the expeditious formation of imines by oxidation of amines with atmospheric oxygen (O 2). Interestingly, the increase of the O 2 pressure from 1 atm to 0.4 MPa promoted the selective oxidation of benzylamine but thereafter declined with a further boost to 0.6 MPa. Notably, an electron transfer between the oxidatively quenched 2H1NA-TiO 2 and TEMPO is established, offering a new pathway for environmental applications. This work presents a strategy in designing cutting-edge visible light photocatalysts via altering semiconductors with surface ligands. [ABSTRACT FROM AUTHOR]
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- 2021
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11. Whole genome resequencing-based analysis of plateau adaptation in Meiren yak (<italic>Bos grunniens</italic>)
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Wang, Tong, Ma, XiaoMing, Ma, ChaoFan, Wu, XiaoYun, ZhaXi, Ta, Yin, LiXin, Li, WeiGuo, Li, YuFei, Liang, ChunNian, and Yan, Ping
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AbstractThe Meiren yak is an important genetic resource in Gansu Province, China. In this study, we aimed to explore the evolutionary history and population structure of the genetic resource of Meiren yak and to mine the characteristic genes of Meiren yak. We analysed a total of 93 yaks of eight yak breeds based on whole genome resequencing combined with population genomics and used θπ ratio and Fst method to screen the selected sites in the genome region. The results proved that Meiren yak can be used as a potential genetic resource in Gansu Province. The genes in Meiren yak with positive selection in selection signal analysis were subjected to the Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses, which indicated that the genes were related to the adaptability to high altitude and hypoxic environment. By analysing the genetic variation of Meiren yak at the genome-wide level, this study provided a theoretical basis for genetic improvement of Meiren yak and for the development of high-quality yak resources. [ABSTRACT FROM AUTHOR]
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- 2024
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12. Genome-Wide Transcriptome Profiling Reveals the Mechanisms Underlying Hepatic Metabolism under Different Raising Systems in Yak.
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Zhang, Mengfan, Zha, Xita, Ma, Xiaoming, La, Yongfu, Guo, Xian, Chu, Min, Bao, Pengjia, Yan, Ping, Wu, Xiaoyun, and Liang, Chunnian
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YAK , *GENE expression , *LIPID metabolism , *MEAT quality , *TRANSCRIPTOMES - Abstract
Simple Summary: Yaks are a major economic source for people in the Tibetan Plateau region. Yaks are rich in nutrients, but their low fat content is not conducive to the large-scale promotion of yak meat, so the study of the mechanism of yak lipid deposition is beneficial to the marketing of yak meat. In this paper, the results of transcriptome sequencing analysis of yak liver showed that it could be determined that the expression levels of genes associated with partial lipid deposition were significantly up-regulated during yak fattening. In addition, this study found that the tenderness of yak meat improved during this process. Fattening significantly affects fat deposition in yaks, which may be realized through its effects on lipid metabolic pathways. Therefore, studying the mechanism of lipid deposition in yaks and fattening yaks will improve the quality of yak meat. Yak meat is nutritionally superior to beef cattle but has a low fat content and is slow-growing. The liver plays a crucial role in lipid metabolism, and in order to determine whether different feeding modes affect lipid metabolism in yaks and how it is regulated, we employed RNA sequencing (RNA-seq) technology to analyze the genome-wide differential gene expression in the liver of yaks maintained under different raising systems. A total of 1663 differentially expressed genes (DEGs) were identified (|log2FC| ≥ 0 and p-value ≤ 0.05), including 698 down-regulated and 965 up-regulated genes. According to gene ontology (GO) and KEGG enrichment analyses, these DEGs were significantly enriched in 13 GO terms and 26 pathways (p < 0.05). Some DEGs were enriched in fatty acid degradation, PPAR, PI3K-Akt, and ECM receptor pathways, which are associated with lipid metabolism. A total of 16 genes are well known to be related to lipid metabolism (e.g., APOA1, FABP1, EHHADH, FADS2, SLC27A5, ACADM, CPT1B, ACOX2, HMGCS2, PLIN5, ACAA1, IGF1, FGFR4, ALDH9A1, ECHS1, LAMA2). A total of 11 of the above genes were significantly enriched in the PPAR signaling pathway. The reliability of the transcriptomic data was verified using qRT-PCR. Our findings provide new insights into the mechanisms regulating yak meat quality. It shows that fattening improves the expression of genes that regulate lipid deposition in yaks and enhances meat quality. This finding will contribute to a better understanding of the various factors that determine yak meat quality and help develop strategies to improve yield and quality. [ABSTRACT FROM AUTHOR]
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- 2024
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13. Proteomic Analysis Reveals the Effects of Different Dietary Protein Levels on Growth and Development of Jersey-Yak.
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Yang, Guowu, Dai, Rongfeng, Ma, Xiaoming, Huang, Chun, Ma, Xiaoyong, Li, Xinyi, La, Yongfu, Dingkao, Renqing, Renqing, Ji, Guo, Xian, Zhaxi, Ta, and Liang, Chunnian
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DIETARY proteins , *PROTEINS in animal nutrition , *PROTEOMICS , *YAK , *JERSEY cattle , *MUSCLE growth , *MYOSIN - Abstract
Simple Summary: The aim of our study was to investigate the effects of dietary supplementation with different protein levels after grazing on growth performance as well as on the proteomics of the LL muscle of Jersey-yak. The results showed that a certain amount of crude protein supplementation to Jersey-yak at the end of grazing significantly improved growth performance and increased their economic efficiency. In addition, we analyzed the proteomic differences in the longest dorsal muscle of Jersey-yak. From our analyses, we identified differences in the abundance of 434 proteins involved in pathways related to muscle growth and development and energy metabolism, among others. Several of the proteins enriched in these pathways, such as MYH8, are associated with myoblast development and differentiation as well as muscle development. In conclusion, the results of this study provide proteomic insights into the different feeding patterns of yak crossbred progeny, suggesting that a greater economic income can be achieved by improving their feeding conditions. Jersey-yak is a hybrid offspring of Jersey cattle and yak (Bos grunniens). Changing the feeding system of Jersey-yak can significantly improve its growth performance. In this study, tandem mass tag (TMT) proteomics technology was used to determine the differentially expressed proteins (DEPs) of the longissimus lumborum (LL) muscle of Jersey-yak fed different protein levels of diet. The results showed that compared with the traditional grazing feeding, the growth performance of Jersey-yaks was significantly improved by crude protein supplementation after grazing. A total of 3368 proteins were detected in these muscle samples, of which 3365 were quantified. A total of 434 DEPs were identified. Through analyses, it was found that some pathways related to muscle growth and development were significantly enriched, such as Rap1 signaling pathway, mTOR signaling pathway, and TGF-beta signaling pathway. A number of DEPs enriched in these pathways are related to muscle cell development, differentiation, and muscle development, including integrin subunit alpha 7 (ITGA7), myosin heavy chain 8 (MYH8), and collagen type XII alpha 1 chain (COL12A1). In conclusion, the results of this study provide insights into the proteomics of different feeding patterns of Jersey-yak, providing a stronger basis for further understanding the biological mechanism of hybrid varieties. [ABSTRACT FROM AUTHOR]
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- 2024
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14. Enhanced visible light photoelectrocatalytic degradation of o-chloronitrobenzene through surface plasmonic Au nanoparticles and g-C3N4 co-modified TiO2 nanotube arrays photoanode.
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Xin, Shuaishuai, Ma, Xiaoming, Lu, Jinren, Zhang, Guangshan, Huo, Siyue, Gao, Mengchun, Xu, Peng, Liu, Wenjie, and Fu, Wenxian
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GOLD nanoparticles , *VISIBLE spectra , *NANOTUBES , *TITANIUM dioxide , *PLASMONICS , *INDUSTRIAL chemistry , *CHEMICAL-looping combustion - Abstract
Chloronitrobenzenes are typical refractory aromatic halogenated nitroaromatic and high-toxic contaminants. The reduction process of chloronitrobenzenes cannot realize their mineralization, and the existence of chlorine group is detrimental to degradation through single chemical oxidation technology. Herein, the Au nanoparticles and graphitic carbon nitride co-modified TiO 2 nanotube arrays (Au/g-C 3 N 4 /TNAs) photoanodes were fabricated to degrade o -chloronitrobenzene (o -CNB) target pollutant in photoelectrocatalytic (PEC) system. The Au/g-C 3 N 4 /TNAs fabricated with 0.20 mM HAuCl 4 0.4 H 2 O (Au/g-C 3 N 4 /TNAs-0.20) had more superior optical and photoelectrochemical properties than other fabricated photoanodes. Au/g-C 3 N 4 /TNAs-0.20 photoanode increased dechlorination efficiency through reduction process, which was beneficial to o -CNB degradation and TOC removal. The coexistence of anions and humic acid inhibited the PEC degradation of o -CNB. The photogenerated electron, •O 2 −, hole and •OH participated in o -CNB degradation in PEC system. The degradation pathway of o -CNB was inferred through GC-MS spectra and DFT calculation. The acute toxicity and bioaccumulation factor of o -CNB were effectively reduced by PEC degradation. [Display omitted] • Au/g-C 3 N 4 /TNAs photoanodes were fabricated for PEC degradation of o -CNB. • The g-C 3 N 4 and Au nanoparticles improved photoelectrochemical properties of TNAs. • Au/g-C 3 N 4 /TNAs photoanode increased dechlorination and TOC removal rate of o -CNB. • Degradation pathways of o -CNB were proposed by GC-MS analysis and DFT calculation. • Overall toxicity of o -CNB was reduced after PEC degradation by Au/g-C 3 N 4 /TNA-0.20. [ABSTRACT FROM AUTHOR]
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- 2023
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15. Inserting acetylene into an olefin-linked covalent organic framework for boosting the selective photocatalytic aerobic oxidation of sulfides.
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Zhang, Fulin, Ma, Xiaoming, Dong, Xiaoyun, Miao, Xin, and Lang, Xianjun
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ACETYLENE , *SULFIDES , *SULFOXIDES , *CHARGE transfer , *CHARGE exchange , *ENERGY transfer , *PHOTOCATALYTIC oxidation - Abstract
[Display omitted] • TBPA-COF is constructed by inserting acetylene into the olefin-linked TBP-COF. • Both TBP-COF and TBPA-COF are observed microspheres. • TBPA-COF is superior to TBP-COF for blue light photocatalysis. • Selective photocatalytic oxidation of sulfides over TBPA-COF is achieved. • Both electron and energy transfer contribute to the formation of sulfoxides. Covalent organic frameworks (COFs) with superior semiconducting properties imparted by sufficient π-conjugations and ordered structures have great potential in visible light photocatalysis. Thus, introducing extra π-conjugated rigid units into the olefin-linked COFs is effective to promote charge transfer and performance. Here, two olefin-linked COFs, TBP-COF and TBPA-COF, were synthesized by reacting 2,4,6-trimethyl-1,3,5-triazine (TMT) with 4,4′-bis(formyl)biphenyl (BFP) and bis(4-formylphenyl)acetylene (BFPA) through organic acid-induced solvothermal condensation. Both TBP-COF and TBPA-COF microspheres were with high crystallinity and uniform micropores. Owing to the insertion of acetylene, TBPA-COF possessed an extended π-conjugated structure that is conducive to smooth charge transfer. Compared to TBP-COF, TBPA-COF delivered superior photocatalytic performance on the selective aerobic oxidation of organic sulfides. Besides, both electron and energy transfer pathways contributed to the formation of sulfoxides over TBPA-COF. This work presents the ascendancy of inserting acetylene into olefin-linked COFs in building efficient visible light photocatalysts for organic oxidations. [ABSTRACT FROM AUTHOR]
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- 2023
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16. The Novel Structural Variation in the GHR Gene Is Associated with Growth Traits in Yaks (Bos grunniens).
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Wang, Fubin, Wu, Xiaoyun, Ma, Xiaoming, Bao, Qi, Zheng, Qingbo, Chu, Min, Guo, Xian, Liang, Chunnian, and Yan, Ping
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YAK , *SOMATOTROPIN receptors , *ANGIOTENSIN converting enzyme , *RUNX proteins , *TRANSCRIPTION factors , *LIVESTOCK growth , *LIVESTOCK breeding - Abstract
Simple Summary: Yak is the dominant animal species in China's plateau regions. The development of the yak industry is conducive to protecting the ecosystem of the Qinghai–Tibet Plateau and promoting local economic development. However, the slow growth and development of yak have seriously affected the development of the yak industry. Structural variation (SV) has been widely applied in livestock breeding for growth traits. Therefore, it is of great significance to use SV to improve yak growth traits in yak breeding. Meanwhile, the GHR gene plays an important role in the formation and normal development of bones. This study associated the correlation between the yak GHR SV gene and growth traits and confirmed that GHR-SV can be used as a molecular marker for the early reproduction of yaks. This study provides a theoretical basis for the early growth and development of yaks. The growth hormone receptor (GHR) is a member of the cytokine/hematopoietic factor receptor superfamily, which plays an important role in the growth and development, immunity, and metabolism of animals. This study identified a 246 bp deletion variant in the intronic region of the GHR gene, and three genotypes, including type II, type ID, and type DD, were observed. Genotype analysis of structural variation (SV) was performed on 585 individuals from 14 yak breeds, and it was found that 246 bp deletion was present in each breed. The II genotype was dominant in all yak breeds except for SB yak. The association analysis of gene polymorphisms and growth traits in the ASD yak population showed that the 246 bp SV was significantly associated with body length at 6 months (p < 0.05). GHR messenger RNA (mRNA) was expressed in all the tested tissues, with significantly higher levels in the liver, muscle, and fat than in other organs. The results of transcription activity showed that the luciferase activity of the pGL4.10-DD vector was significantly higher than that of the pGL4.10-II vector (p < 0.05). Additionally, the transcription-factor binding prediction results showed that the SV in the runt-related transcription factor 1 (Runx1) transcription-factor binding site may affect the transcriptional activity of the GHR gene, regulating yak growth and development. This study showed that the novel SV of the GHR gene could be used as a candidate molecular marker for the selection of the early growth trait in ASD yak. [ABSTRACT FROM AUTHOR]
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- 2023
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17. Anchoring dye onto 1D Nb2O5 in cooperation with TEMPO for the selective photocatalytic aerobic oxidation of amines.
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Ma, Xiaoming, Li, Xia, Zhou, Jun, Wang, Yuexin, and Lang, Xianjun
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PHOTOCATALYTIC oxidation , *ALCOHOL oxidation , *CHEMICAL amplification , *SEMICONDUCTOR materials , *SEMICONDUCTOR design , *AMINES , *SECONDARY amines , *NITROXIDES - Abstract
[Display omitted] • Semiconductor materials are the key to anchor visible light-active dye molecules. • 1D Nb 2 O 5 nanorods with a specific surface area of 264.3 m2•g−1 are synthesized. • Dye-Nb 2 O 5 nanorods can facilitate the green light-driven selective oxidation of amines in air. • TEMPO enhances photocatalytic efficiency. • Superoxide (O 2 •−) is the central reactive oxygen species. Visible light-driven photocatalysis over semiconductors has emerged as one of the most desired strategies for animating diverse selective chemical transformations. Herein, one-dimensional (1D) Nb 2 O 5 nanorods with a specific surface area of 264 m2•g−1 were synthesized via a straightforward hydrothermal method. Thereafter, an efficient visible light photocatalyst was constructed by in-situ anchoring of alizarin red S (ARS) onto the Nb 2 O 5 nanorods to afford ARS-Nb 2 O 5. Resultantly, the ARS-Nb 2 O 5 nanorods can facilitate the selective aerobic oxidation of amines into imines, affording about twice of the result over 3D hierarchical Nb 2 O 5. Notably, 2,2,6,6-tetramethylpiperidine- N -oxyl (TEMPO) could synchronize the intricate electron and proton transfers between ARS-Nb 2 O 5 nanorods, amines, and superoxide (O 2 •−). In most cases, the green light-driven primary and secondary amines into imines could be completed with about 95% selectivity within 2 h. This work highlights the dimension of designing semiconductor materials to anchor visible light-active molecules for selective oxidation reactions under benign conditions. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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18. Selective photocatalytic formation of sulfoxides by aerobic oxidation of sulfides over conjugated microporous polymers with thiazolo[5,4‑d]thiazole linkage.
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Li, Xia, Ma, Xiaoming, Zhang, Fulin, Dong, Xiaoyun, and Lang, Xianjun
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CONJUGATED polymers , *SULFOXIDES , *PHOTOCATALYSTS , *SULFIDES , *OXIDATION - Abstract
[Display omitted] • Thiazolo[5,4- d ]thiazole (TzTz) is employed as the linkage to synthesize CMPs. • The TzTz-linked CMPs are thoroughly characterized. • TzTz-CMP-3 displays excellent photocatalytic activity for selective aerobic oxidation. • The photocatalytic properties of TzTz-linked CMPs could be tailored by molecular symmetry. • TzTz-CMP-3 with unprecedent activity is uncovered for the formation of sulfoxides. Developing selective formation of sulfoxides by aerobic oxidation of sulfides over highly efficient visible-light photocatalysts is an essential but challenging task. Herein, a conjugated microporous polymer (CMP) photocatalyst with the desired linkage thiazolo[5,4‑ d ]thiazole (TzTz) is presented to undertake this task. Remarkably, a catalyst-free solvothermal process afforded TzTz-CMP-3 from tetra(4-formylphenyl)-benzene and dithiooxamide. The photocatalytic activity of TzTz-CMP-3 is demonstrated with the blue light-powered selective formation of sulfoxides by aerobic oxidation of sulfides. A wide range of sulfoxides can be effectively formed by oxidation of sulfides with excellent product selectivities (up to 99 %) over the TzTz-CMP-3 photocatalyst. The more symmetric π-conjugated aromatic ring of TzTz-CMP-3 endows it with more favorable activity than the less symmetric ones. This work underscores the symmetry of building blocks for CMPs for selective photocatalytic formation of valuable organic molecules. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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19. Analysis of Copy Number Variation in the Whole Genome of Normal-Haired and Long-Haired Tianzhu White Yaks.
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Meng, Guangyao, Bao, Qi, Ma, Xiaoming, Chu, Min, Huang, Chun, Guo, Xian, Liang, Chunnian, and Yan, Ping
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YAK , *INTEGERS , *HAIR growth , *PHENOTYPIC plasticity , *GERMPLASM - Abstract
Long-haired individuals in the Tianzhu white yak population are a unique genetic resource, and have important landscape value. Copy number variation (CNV) is an important source of phenotypic variation in mammals. In this study, we used resequencing technology to detect the whole genome of 10 long-haired Tianzhu white yaks (LTWY) and 10 normal-haired Tianzhu white yaks (NTWY), and analyzed the differences of CNV in the genome of LTWYs and NTWYs. A total of 110268 CNVs were identified, 2006 CNVRs were defined, and the distribution map of these CNVRs on chromosomes was constructed. The comparison of LTWYs and NTWYs identified 80 differential CNVR-harbored genes, which were enriched in lipid metabolism, cell migration and other functions. Notably, some differential genes were identified as associated with hair growth and hair-follicle development (e.g., ASTN2, ATM, COL22A1, GK5, SLIT3, PM20D1, and SGCZ). In general, we present the first genome-wide analysis of CNV in LTWYs and NTWYs. Our results can provide new insights into the phenotypic variation of different hair lengths in Tianzhu white yaks. [ABSTRACT FROM AUTHOR]
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- 2022
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20. Intravoxel Incoherent Motion Diffusion-Weighted Imaging and 3D-ASL to Assess the Value of Ki-67 Labeling Index and Grade in Glioma.
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Zhou, Jian, Li, Huafeng, Ma, Xiaoming, Jin, Miao, Meng, Xin, and Zhang, Guangfeng
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GLIOMAS , *KI-67 antigen , *DIFFUSION magnetic resonance imaging , *CEREBRAL circulation , *CENTRAL nervous system diseases , *MAGNETIC resonance angiography , *MANN Whitney U Test , *COBALT - Abstract
Objective. To determine the proportion of intravoxel incoherent motion diffusion-weighted images (IVIM-DWI) and three-dimensional arterial circulation markers (3D-ASL) in Ki-67 labeling index (Ki-67 LI) and glioma grading. Methods. According to the classification of diseases of the central nervous system dealt with by WHO in 2007, patients with stage II glioma were classified as low (n = 20) and patients with stages III-IV were divided into higher levels (n = 22). Prior to surgery, brain MRI, IVIM-DWI, and 3D-ASL were performed in all patients, and the actual water molecular diffusion coefficient (D), microcirculation coefficient (D ∗), blood flow fraction (f), and cerebral blood flow (CBF) were measured. A rank sum (Mann–Whitney U test) was used to compare the four upper and lower level Ki-67 LI measurements. Spearman's method is used to identify the relationship between 4 groups of quantification and Ki-67 LI. Reciprocal grafting (ROC) curves were used to measure the diagnosis of four groups of glioma grading defects. Results. There were significant differences in D , D ∗ , f , and CBF between the solid region of the tumor and the normal white matter contralateral to it (P < 0.05). The significant differences of r D , r D ∗ , r f , and r CBF were shown between patients with low-grade glioma and high-grade glioma (P < 0.05). Ki-67 LI was found to have negative correlation with r D (r = 00.693 , P < 0.001) and r f (r = 00.539 , P < 0.001), but similarly correlated with r CBF (r = 0.665 , P < 0.001) in patients with glioma. Recipient efficacy for predicting advanced and secondary glioma from r D , r f , r D ∗ , r CBF, and Ki-67 LI raises AUCs of 0.819, 0.747, 0.719, 0.836, and 0.907, respectively. Conclusion. IVIM-DWI has good application value for preoperative grading of glioma. [ABSTRACT FROM AUTHOR]
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- 2022
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21. Serum Metabolomic Analysis of Synchronous Estrus in Yaks Based on UPLC-Q-TOF MS Technology.
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Feng, Fen, Huang, Chun, Luosang, Dunzhu, Ma, Xiaoming, La, Yongfu, Wu, Xiaoyun, Guo, Xian, Pingcuo, Zhandui, and Liang, Chunnian
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ESTRUS , *LIQUID chromatography-mass spectrometry , *YAK , *BLOOD serum analysis , *GONADOTROPIN releasing hormone , *ESTRUS synchronization - Abstract
Simple Summary: The synchronization process of yak estrus plays a crucial role in enhancing the reproductive success rate of yaks and safeguarding the continuation of the species. Metabolomics, a branch of bio-chemistry, primarily focuses on the quantitative analysis of all metabolites within an organism, aiming to identify the relative relationships between metabolites and physiological changes. It serves as a valuable tool for delving into the molecular mechanisms and responses of metabolic pathways under various perturbations. In this study, metabolomics methods were utilized to analyze blood samples collected from non-estrus yaks as well as those that underwent hormonally induced synchronized estrus to identify differential metabolites. The research results provide a theoretical basis for the optimization and application of synchronized estrus technology in yaks, promoting the healthy development of the yak industry. The yak is a unique species of livestock found in the Qinghai-Tibet Plateau and its surrounding areas. Due to factors such as late sexual maturity and a low rate of estrus, its reproductive efficiency is relatively low. The process of estrus synchronization in yaks plays a crucial role in enhancing their reproductive success and ensuring the continuation of their species. In order to clarify the characteristics of the serum metabolites of yak estrus synchronization, the yaks with inactive ovaries were compared with the estrus synchronization yaks. In this study, yaks were divided into the inactive ovaries group (IO), gonarelin-induced yak estrus group (GnRH), and chloprostenol sodium-induced yak estrus group (PGF). After the completion of the estrus synchronization treatment, blood samples were collected from the jugular veins of the non-estrus yaks in the control group and the yaks with obvious estrus characteristics in the GnRH and PGF groups. Metabolites were detected by ultra-high performance liquid chromatography-mass spectrometry, and differential metabolites were screened by multivariate statistical analysis. The results showed that a total of 70 significant differential metabolites were screened and identified in the GnRH vs. IO group, and 77 significant differential metabolites were screened and identified in the PGF vs. IO group. Compared with non-estrus yaks, 36 common significant differential metabolites were screened out after the induction of yak estrus by gonarelin (GnRH) and cloprostenol sodium (PGF), which were significantly enriched in signaling pathways such as the beta oxidation of very long chain fatty acids, bile acid biosynthesis, oxidation of branched chain fatty acids, steroidogenesis, steroid biosynthesis, and arginine and proline metabolism. This study analyzed the effects of gonadotropin releasing hormone (GnRH) and prostaglandin F (PGF) on the reproductive performance of yaks treated with estrus synchronization, which provides a theoretical basis for the optimization and application of yak estrus synchronization technology and promotes the healthy development of the yak industry. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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22. Age-dependent changes in the expression and localization of LYZL4, LYZL6 and PCNA during testicular development in the Ashidan yak.
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La, Yongfu, Li, Zhongbang, Ma, Xiaoming, Bao, Pengjia, Chu, Min, Guo, Xian, Liang, Chunnian, and Yan, Ping
- Abstract
AbstractLysozyme like 4 (LYZL4), lysozyme like 6 (LYZL6) and proliferating cell nuclear antigen (PCNA) are implicated in the regulation of testicular function, but there was no research reported available on the expression patterns of
LYZL4 ,LYZL6 andPCNA genes at different developmental stages of yak testes. In this study, we used the qRT-PCR, western blotting and immunohistochemistry estimated theLYZL4 ,LYZL6 andPCNA gene expression and protein lo-calization at different developmental stages of yak testes. The qPCR results showed that the mRNA expression ofLYZL4 ,LYZL6 andPCNA genes significantly increased with age in the testes of yaks. Western blot results showed that the protein abundance of LYZL4, LYZL6 and PCNA in yak testes was significantly higher after puberty than before puberty. Furthermore, the results of immunohistochemistry indicated that LYZL4, LYZL6 and PCNA may be involved in the regulation of spermatogonia proliferation and Leydig cell function in immature testis. In adult yak testes, LYZL4, LYZL6 and PCNA may involve in the development of round spermatids and primary spermatocytes during testicular development. Our results indicated that LYZL4, LYZL6 and PCNA may be involved in the development of Sertoli cells, Leydig cells and gonocytes in yak testes. [ABSTRACT FROM AUTHOR]- Published
- 2024
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23. Early Growth and Development and Nonlinear Model Fitting Analysis of Ashidan Yak.
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Meng, Guangyao, La, Yongfu, Bao, Qi, Wu, Xiaoyun, Ma, Xiaoming, Huang, Chun, Chu, Min, Liang, Chunnian, and Yan, Ping
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YAK , *CHEST (Anatomy) , *BODY weight , *BODY size , *ANIMAL genetics - Abstract
Simple Summary: Ashidan yak is a new breed of hornless yak developed by Chinese scientists, which has an important economic value. However, little is known about the growth of Ashidan yaks. This study analyzed the body weight and body size measurements of 260 female Ashdan yaks and compared the performance of five nonlinear models (Logistic model, Gompertz model, Brody model, von Bertalanffy model and Richards model). Our results showed that the early growth and development of Ashidan yak change with the seasons, and the Richards model performs better among the five models. Understanding animal growth plays an important role in improving animal genetics and breeding. In order to explore the early growth and development law of Ashidan yak, the body weight (BW), wither height (WH), body oblique length (BL) and chest girth (CG) of 260 female Ashidan yaks were measured. These individuals grew under grazing conditions, and growth traits were measured at 6, 12, 18 and 30 months of age. Then the absolute growth and relative growth of Ashidan yak were calculated, and five nonlinear models (Logistic model, Gompertz model, Brody model, von Bertalanffy model and Richards model) were used to fit the growth curve of Ashidan yak. The fitting effect of the model was evaluated according to MSE, AIC and BIC. The results showed that the growth rate of Ashidan yak was the fastest from 12 to 18 months old, and the growth was slow or even stagnant from 6 to 12 months old. The AIC and BIC values of the Richards model were the lowest among the five models, with an AIC value of 4543.98 and a BIC value of 4563.19. The Richards model estimated body weight at 155.642 kg. In summary, the growth rate of female Ashidan yak changes with the seasons, growing faster in warm seasons and slower in cold seasons. Richards model is the best model to describe the growth curve of female Ashidan yak in five nonlinear models. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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24. Micro-structure and morphology of tailings sand under different oxidation and acidification degree.
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Wang, Gang, Liu, Xiqi, Song, Leibo, Ma, Xiaoming, Chen, Wenzhao, and Qiao, Jiaxing
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ACIDIFICATION , *FRACTAL dimensions , *MICROSTRUCTURE , *SAND , *SAND filtration (Water purification) , *OXIDATION , *COHESION , *INTERNAL friction - Abstract
The tailings pond is a dangerous source of man-made debris flow with high potential energy. The oxidative acidification of tailings may cause the instability of the pond and induce serious safety accidents. The influence of oxidation and acidification degree on macro mechanical properties of tailings is discussed from the aspects of mineral composition and microstructure. The results show that as the degree of oxidation and acidification of tailings sand increases, the overall structural performance and load-bearing capacity decrease, and its cohesion (c) and internal friction angle (φ) show a decreasing trend. In fact, the engineering properties of tailings with different oxidation and acidification degrees are dominated by the physicochemical composition and structural characteristics. On the one hand, as the degree of oxidation increases, acidic substance will neutralize with CaCO3 and CaMg(CO3)2, resulting in the loss of cemented substance and the decrease of cementation force between tailing sand particles as well as the gradual destruction of the integrity of tailing sand. On the other hand, the increase of oxidation and acidification degree of tailing sand leads to a gradual reduction of outline (2D) fractal dimension and gray surface (3D) fractal dimension of surface laminated structure as well as the obvious reduction of laminated structure and its roughness of tailings sand. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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25. The combination of in situ photodynamic promotion and ion-interference to improve the efficacy of cancer therapy.
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Yang, Pengfei, Chang, Yi, Zhang, Jie, Gao, Fangli, Liu, Xinhe, Wei, Qingcong, Ma, Xiaoming, and Guo, Yuming
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CANCER treatment , *CALCIUM ions , *PHOTODYNAMIC therapy , *TUMOR growth , *TUMOR treatment - Abstract
[Display omitted] Photodynamic therapy (PDT) is proved to be a promising modality for clinical cancer treatment. However, it also suffers from a key obstacle in association with its oxygen-dependent nature which greatly limits its effective application against hypoxic tumors. Herein, on the basis of the unique property of calcium peroxide (CaO 2), we propose an O 2 -self-supply strategy for the promotion of PDT by combining the in situ O 2 -generation characteristic of calcium peroxide with the photosensitive nature of porphyrin. A shell of ZIF-8 was synthesized surround the CaO 2 core to prevent the CaO 2 from premature decomposition and increased the loading of THPP efficiently. Depending on the in situ self-supply of O 2 , the photosensitizer was able to exhibit an enhanced PDT effect that significantly inhibit the growth of tumor. Moreover, the enrichment of free calcium ions derived from the decomposition of CaO 2 under acidic tumor microenvironment also shows the unique ion-interference effect and contributes to the obvious inhibition against tumor growth. This work presents a synergistic strategy for the construction of a photodynamic promotion/ion-interference combined nano-platform which can also serve as an inspiration for the future design of effective nanocomposites in tumor treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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26. Comparative Study of the Expression Profiles of miRNAs of Milk-Derived Exosomes of Yak and Jeryak.
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Ren, Wenwen, Zhang, Yongfeng, Dingkao, Renqing, Huang, Chun, Ma, Xiaoming, Wu, Xiaoyun, La, Yongfu, Chu, Min, Bao, Pengjia, Guo, Xian, Pei, Jie, Yan, Ping, and Liang, Chunnian
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EXOSOMES , *YAK , *GENE expression , *MICRORNA , *DENSITY gradient centrifugation , *NON-coding RNA , *MILKFAT - Abstract
Simple Summary: Exosomes are multivesicular bodies produced and secreted by living cells as membranous vesicles with a diameter of about 30–150 nm, density of 1.13–1.19 g/mL, and a typical "cup dish" shape. Exosomes carry proteins, miRNA, lncRNA, circRNA, and mRNA and their degradation fragments are involved in intracellular signal transduction important for regulation of cellular activities. Of these, miRNAs play an important role in the regulation of gene expression in important biological processes such as cell division, tissue development, and immune response. In this study, milk quality was determined and exosome miRNAs from yak and Jeryak milk sampled in June and December were characterized by sequencing. Differentially expressed miRNAs (DEMs) and pathways related to lactation performance were identified. In summary, this study provides data that can be used to develop alternative strategies for improving the lactation performance of yaks and Jeryaks. The Jeryak is the hybrid offspring of yaks and Jersey cattle and exhibit improved milk and meat yields. Biomolecules carried within milk exosomes are important for cell growth, development, immune regulation, and various pathophysiological processes. Previous studies showed that miRNAs regulate mammary gland development, lactation, and milk quality. This study explored the relationship between milk exosomes miRNAs and lactation performance. A comparison of the milk content showed that yak milk was of a better quality compared to Jeryak milk (casein, fat, TS, SNF, lactose). Milk collected in December was superior to that collected in June for both yak and Jeryak, except for lactose concentrations. Exosomes were extracted by density gradient centrifugation and miRNA expression profiles in milk exosomes from three yaks and three Jeryaks collected in June and December were detected by small RNA sequencing. In all, 22, 120, 78, and 62 differentially expressed miRNAs (DEMs) were identified in Jun_ JY vs. Jun_ Y (P1: Jeryak in June vs. Yak in June), Jun_ JY vs. Dec_ JY (P2: Jeryak in June vs. Jeryak in December), Dec_ JY vs. Dec_ Y (P3: Jeryak in December vs. Yak in December), and Jun_ Y vs. Dec_ Y (P4: Yak in June vs. Yak in December) groups. These DEMs were enriched in functions and signaling pathways related to lactation performance. In conclusion, these findings are a reference tool to study the molecular basis of lactation performance. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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27. Copy Number Variation of the SOX6 Gene and Its Associations with Growth Traits in Ashidan Yak.
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Li, Xinyi, Huang, Chun, Liu, Modian, Dai, Rongfeng, Wu, Xiaoyun, Ma, Xiaoming, Chu, Min, Bao, Pengjia, Pei, Jie, Guo, Xian, Yan, Ping, and Liang, Chunnian
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SOX transcription factors , *YAK , *CHEST (Anatomy) , *GENE expression , *TRANSCRIPTION factors - Abstract
Simple Summary: The SOX6 (sex determining region Y-box 6) gene belongs to one of the transcription factors in the SRY (sex-determining region Y) family, which affects sex determination, embryonic and nervous system development, bone and various organ formation. In the previous study, the whole-genome sequencing was used to detect multiple genes located in the copy number variation region, including SOX6 gene. In this study, we identified the correlation between the growth traits and CNV of SOX6 in 311 Ashidan yaks. The results showed that SOX6-CNV was significantly correlated with the chest girth of the 6-months old yaks (p < 0.05) and 30-months yaks (p < 0.05), and withers height of 6 months yaks (p < 0.05) and 18-months yaks (p < 0.05), suggesting the SOX6-CNV affect growth traits in yaks, and could be new markers for the selection of yak breeding. Copy number variation (CNV) is a fundamental type of structural variation of the genome affecting the economic traits of livestock. The SOX6 gene (sex-determining region Y-box 6), as a transcription factor, has multiple functions with regard to sex determination, embryonic growth, the nervous system development, as well as bone, and various organ formation. This study employed quantitative real-time fluorescence quota PCR (qPCR) for detecting the SOX6-CNV of the 311 Ashidan yaks and analyzed the correlation of the SOX6-CNV with four phenotypes (including body weight, withers height, body length, and chest girth) of the yaks aged 6, 12, 18, and 30 months using ANOVA and multiple comparisons. Furthermore, the SOX6 gene expression was identified in seven different tissues of the yaks. The experiment results demonstrated the expression of SOX6 in each tissue, and the kidney and muscle tissue were found to have higher relative expression levels. Based on the processing by IBM SPSS software, SOX6-CNV was significantly correlated with the chest girth of the 6-months old yaks (p < 0.05) and 30-months yaks (p < 0.05), and withers height of 6 months yaks (p < 0.05) and 18-months yaks (p < 0.05), as well as the normal type of CNV, was chosen for yak breeding. In conclusion, SOX6 might be prominently involved in promoting growth and development of yaks, suggesting that the SOX6 gene can be used in breeding yaks by molecular marker-assisted selection (MAS). The study also offered some important insights into the references and clues for the genetic breeding of yaks. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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28. Transcriptome-Wide Study of mRNAs and lncRNAs Modified by m 6 A RNA Methylation in the Longissimus Dorsi Muscle Development of Cattle-Yak.
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Huang, Chun, Dai, Rongfeng, Meng, Guangyao, Dingkao, Renqing, Wang, Xingdong, Ren, Wenwen, Ma, Xiaoming, Wu, Xiaoyun, Chu, Min, La, Yongfu, Bao, Pengjia, Guo, Xian, Pei, Jie, Yan, Ping, and Liang, Chunnian
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MUSCLE growth , *RNA methylation , *LINCRNA , *ERECTOR spinae muscles , *GENE expression - Abstract
Cattle-yak is a hybrid F1 generation of cattle and yak, which has a history of more than 3000 years and has shown better production performance and higher economic benefits than those of yaks. However, up to now, there has been no study on the transcriptome-wide m6A methylation profile of bovine skeletal muscle and its potential biological function during muscle development. Here, we observed significant changes in the expression levels of muscle-related marker genes and methylation-related enzymes during the development of cattle-yak, and the overall m6A content in the Longissimus dorsi muscle of 18-month-old cattle-yak decreased significantly. A total of 36,602 peaks, 11,223 genes and 8388 lncRNAs were identified in the two groups, including 2989 differential peaks (427 up-regulated peaks and 2562 down-regulated peaks), 1457 differentially expressed genes (833 up-regulated genes and 624 down-regulated genes) and 857 differentially expressed lncRNAs (293 up-regulated lncRNAs and 564 down-regulated lncRNAs). GO and KEGG analysis revealed that they were significantly enriched in some muscle-related pathways (Wnt signaling pathway and MAPK signaling pathway) and high-altitude adaptation-related pathway (HIF-1 signaling pathway). Moreover, m6A abundance was positively correlated with gene expression levels, while it was negatively correlated with lncRNA expression levels. This indicates that m6A modification played an important role in the Longissimus dorsi muscle development of cattle-yak; however, the regulation mechanism of m6A-modified mRNA and lncRNA may be different. This study was the first report of transcriptome-wide m6A-modified mRNAs and lncRNAs atlas in the Longissimus dorsi muscle development of cattle-yak, one which will provide new perspectives for genetic improvement in bovines. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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29. Association of HSF1 gene copy number variation with growth traits in the Ashidan yak.
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Ren, Wenwen, Huang, Chun, Ma, Xiaoming, La, Yongfu, Chu, Min, Guo, Xian, Wu, Xiaoyun, Yan, Ping, and Liang, Chunnian
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YAK , *HEAT shock factors , *POLYMERASE chain reaction , *GENES , *GENE expression - Abstract
• The CNV of the HSF1 gene was detected in Ashidan yaks. • There were significant correlations between HSF1-CNV and growth traits of yaks. • The expression level of the HSF1 gene was variable in different tissues of yaks. Copy Number Variation (CNV) is the major manner for the variation of genome structure, which is associated with numerous important traits. The heat shock factor 1 (HSF1) gene is a stress response transcriptional regulator. It participates in the heat shock response, simultaneously participated in the development of tissue. The objective of this research was to explore the influence of CNV of the HSF1 gene on the growth traits of the Ashidan yak. In this study, the growth traits (withers height, body weight, chest girth, and body length) of 274 Ashidan yaks were divided into four stages (6, 12, 18, and 30 months old). Moreover, quantitative polymerase chain reaction (qPCR) was exploited for determining the HSF1 gene relative expression level, and SPSS software was utilized for the statistical analysis. The outcomes indicated that HSF1 -CNV was significantly associated with body length (p < 0.05) and was extremely significant associated with withers height (p < 0.01) of 18-month-old Ashidan yaks. Besides, the HSF1 relative expression in heart and muscle was higher than that existed in other tissues (p < 0.01). The outcomes suggested that the CNV of HSF1 gene would affect the growth and development of the Ashidan yak, which is conducive to the early breeding of yak. [ABSTRACT FROM AUTHOR]
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- 2022
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30. Fine-Grained Butterfly Recognition via Peer Learning Network with Distribution-Aware Penalty Mechanism.
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Xu, Chudong, Cai, Runji, Xie, Yuhao, Cai, Huiyi, Wang, Min, Gao, Yuefang, and Ma, Xiaoming
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INTERACTIVE learning , *BUTTERFLIES , *PRODUCTION management (Manufacturing) , *SPECIES distribution - Abstract
Simple Summary: Automatic species recognition, such as butterflies or other insects, plays a crucial role in intelligent agricultural production management and the study of species diver-sity. However, the quite diverse and subtle interspecific differences and the long-tailed distribution of sample data in fine-grained species recognition are insufficient to learn robust feature representation and alleviate the bias and variance problems of the long-tailed classifier on insect recognition. The objective of this study is to develop a peer learning network with a distribution-aware penalty mechanism proposed to learn discriminative feature representation and mitigate the bias and variance problems in the long-tailed distribution. The results of various contrast experiments on collecting the butterfly-914 dataset show that the proposed PLN-DPM has a higher Rank-1 ac-curacy rate (86.2% on the butterfly dataset and 73.51% on the IP102 dataset). Addi-tionally, we deployed the PLN-DPM model on the smartphone app for butterfly recognition in a real-life environment. Automatic species recognition plays a key role in intelligent agricultural production management and the study of species diversity. However, fine-grained species recognition is a challenging task due to the quite diverse and subtle interclass differences among species and the long-tailed distribution of sample data. In this work, a peer learning network with a distribution-aware penalty mechanism is proposed to address these challenges. Specifically, the proposed method employs the two-stream ResNeSt-50 as the backbone to obtain the initial predicted results. Then, the samples, which are selected from the instances with the same predicted labels by knowledge exchange strategy, are utilized to update the model parameters via the distribution-aware penalty mechanism to mitigate the bias and variance problems in the long-tailed distribution. By performing such adaptive interactive learning, the proposed method can effectively achieve improved recognition accuracy for head classes in long-tailed data and alleviate the adverse effect of many head samples relative to a few samples of the tail classes. To evaluate the proposed method, we construct a large-scale butterfly dataset (named Butterfly-914) that contains approximately 72,152 images belonging to 914 species and at least 20 images for each category. Exhaustive experiments are conducted to validate the efficiency of the proposed method from several perspectives. Moreover, the superior Top-1 accuracy rate (86.2%) achieved on the butterfly dataset demonstrates that the proposed method can be widely used for agricultural species identification and insect monitoring. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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31. Copy Number Variations in the MICALL2 and MOGAT2 Genes Are Associated with Ashidan Yak Growth Traits.
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Liu, Modian, Huang, Chun, Dai, Rongfeng, Ren, Wenwen, Li, Xinyi, Wu, Xiaoyun, Ma, Xiaoming, Chu, Min, Bao, Pengjia, Guo, Xian, Pei, Jie, Xiong, Lin, Yan, Ping, and Liang, Chunnian
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YAK , *LIVESTOCK breeding , *GENE rearrangement , *GENES , *MITOCHONDRIAL proteins , *ACYLTRANSFERASES - Abstract
Simple Summary: Yaks are among the largest high-altitude mammals in the world, and they are ideally adapted to the harsh environmental conditions of the plateau regions. Yaks are thus central to the lives of herdsmen and other local populations in these high-altitude areas. Copy number variations (CNVs) are an important cause of genomic variation in livestock and identifying advantageous CNVs can aid in livestock breeding efforts. In this study, an association between CNVs in the MICALL2 and MOGAT2 genes and Ashidan yak growth traits was confirmed, providing a theoretical foundation for Ashidan yak breeding and meat production efforts. Copy number variations (CNVs) are a result of genomic rearrangement affecting DNA regions over 1 kb in length, and can include inversions, translocations, deletions, and duplications. The molecule interacting with CasL-like protein 2 (MICALL2) gene is primarily associated with mitochondrial protein targeting and exhibits predicted stress fiber colocalization. The monoacylglycerol O-acyltransferase 2 (MOGAT2) gene encodes an enzyme responsible for catalyzing diacylglycerol synthesis from 2-monoacylglycerol and fatty acyl-CoA. For this study, blood samples were obtained from 315 yaks, and the body weight, body length, withers height, and chest girth of these animals were measured at 6, 12, 18, and 30 months of age. Genomic DNA was harvested from the collected blood samples, and CNVs in these samples were detected by qPCR. The resultant data were compared using ANOVAs, revealing significant associations between MICALL2 gene CNVs and body weight at 6 months of age (p < 0.05), body length and chest girth at 30 months of age (p < 0.05), and withers height at 18 months of age (p < 0.01) in Ashidan yaks. Similarly, MOGAT2 CNVs were significantly associated with body weight at 6 and 30 months of age (p < 0.05), and with withers height at 18 months of age (p < 0.01) in these Ashidan yaks. MICALL2 and MOGAT2 gene expression was further analyzed in yak tissue samples, revealing that MICALL2 was most highly expressed in the adipose tissue, whereas MOGAT2 was most highly expressed in the lung. These results thus confirmed the relationship between CNVs in the MICALL2 and MOGAT2 genes and Ashidan yak growth traits, providing a valuable gene locus that can be leveraged for future marker-assisted yak breeding efforts. [ABSTRACT FROM AUTHOR]
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- 2022
- Full Text
- View/download PDF
32. Carbon inequality in global trade: Evidence from the mismatch between embodied carbon emissions and value added.
- Author
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Wang, Yihan, Xiong, Siqin, and Ma, Xiaoming
- Subjects
- *
CARBON emissions , *INTERNATIONAL trade , *ENVIRONMENTAL indicators , *EQUALITY , *CARBON , *COLLOIDAL carbon - Abstract
Global trade not only brings about the exchange of goods and services, but also stimulates the transfer of carbon emissions. Given different economic development stages and industrial structures, global carbon inequality occurs due to the mismatch between trade-related carbon emissions and value added. Combining the multi-regional input-output model and regional environmental index (REI), this paper constructs an integrated research framework to trace the embodied carbon emissions and value-added among six regions from 2010 to 2015 to shed light on global carbon inequality from a multi-regional perspective. The results indicate that major carbon exporters are placed in inferior positions in global trade, rendering them victims of carbon inequality. The EU, Japan and the USA have been favored by global trade from both economic and environmental perspectives, and the advantaged position of the EU has improved whereas the positions of Japan and the USA have slightly decreased. For disadvantage regions, China and ASEAN10 has improved their situations, yet the inequality suffered by the RoW148 has deteriorated. Adjusting the export structure from primary to advanced products is an effective measure to mitigate global carbon inequality. Our major implications help strike thinking on the rationality of global trade and offer integral solutions towards sustainable development. • Embodied carbon emissions and value added are traced based on the MRIO model • An optimized REI index is established to identify carbon inequality from a multi-regional perspective • Carbon inequality in global trade and its sectoral contributors are explored • Export structure changing from primary to advanced products can alleviate carbon inequality [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
33. Fenton-like reaction and glutathione depletion by chiral manganese dioxide nanoparticles for enhanced chemodynamic therapy and chemotherapy.
- Author
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Gao, Fangli, Sun, Mengmeng, Zhang, Jie, Chang, Yi, Gao, Weihua, Ma, Guanglei, Ma, Xiaoming, and Guo, Yuming
- Subjects
- *
MANGANESE dioxide , *GLUTATHIONE , *SEMICONDUCTOR nanoparticles , *CANCER chemotherapy , *NANOPARTICLES , *SELF-healing materials - Abstract
[Display omitted] Chirality-based nanomaterials, especially semiconductors nanoparticles (NPs), are the emerging research in biomedicine field. Herein, chiral manganese dioxide (L/D-MnO 2) NPs were synthesized by using threonine molecules as chiral ligands. Then cisplatin loaded L/D-MnO 2 (L/D-MnO 2 @Pt) NPs were successfully constructed based on the high specific surface area of L/D-MnO 2 NPs. L/D-MnO 2 @Pt NPs could be specifically internalized by tumor cells and efficiently deplete the glutathione (GSH) through redox reaction to release Mn2+ and Pt. The released Mn2+ exhibited strong chemodynamic effect through Fenton-like reaction. The depletion of GSH further improved the chemodynamic therapy (CDT) efficiency. The combination of the CDT of Mn2+ with the chemotherapy of Pt considerably enhanced the tumor therapy efficiency. It was particularly noteworthy that L/D-MnO 2 @Pt NPs showed chirality-based different internalization by tumor cells and further led to different tumor cell ablation effects, in which D-MnO 2 @Pt NPs exhibited stronger therapeutic efficiency than L-MnO 2 @Pt NPs. These findings provided deep insights for novel biomedical applications of chirality-based semiconductors NPs. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
34. Mechanistic insight into pH-dependent adsorption and coprecipitation of chelated heavy metals by in-situ formed iron (oxy)hydroxides.
- Author
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Yang, Zhengheng, Ma, Jinxing, Liu, Fang, Zhang, Hailong, Ma, Xiaoming, and He, Di
- Subjects
- *
INDUSTRIAL metals , *HYDROXIDES , *STABILITY constants , *IRON , *INDUSTRIAL wastes , *ARSENIC removal (Water purification) , *HEAVY metals , *COPPER - Abstract
[Display omitted] Fe(III) coagulation-precipitation process has been widely used to remove heavy metals from industrial effluents; however, the influences of organic ligands on the sequestration of different heavy metals in the Fe(III)/metal-EDTA ternary system are not well understood. In this study, the pH-dependent mechanisms of CuII-EDTA and NiII-EDTA removed by in-situ formed iron (oxy)hydroxides were studied using surface complex modeling and a suite of characterization techniques. Results of surface complex model indicated that there should be minimal difference between removal of CuII-EDTA and NiII-EDTA by iron (oxy)hydroxides if adsorption was the dominant mechanism. However, through the speciation analysis and characterization of the precipitates generated after coagulation and precipitation, we have demonstrated that at neutral pH the complexation of Fe(III) and EDTA influenced the surface properties of iron (oxy)hydroxides formed, with the higher removal of Cu2+ (compared to Ni2+) contributed by its coprecipitation with Fe(III). Moreover, at basic pH, decomplexation of CuII-EDTA occurred on the iron (oxy)hydroxides surface with the released copper ions involved in the formation of (oxy)hydroxides. The low removal of nickel (from NiII-EDTA) was ascribed to the higher conditional stability constant of NiII-EDTA. Results of this study have advanced our understanding of the complicated interactions among Fe(III), organic ligands and heavy metals in the industrial effluents, and provide insight to optimization of the process efficiency. [ABSTRACT FROM AUTHOR]
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- 2022
- Full Text
- View/download PDF
35. Cooperative photocatalysis of dye-TiO2 nanotubes with TEMPO+BF4− for selective aerobic oxidation of amines driven by green light.
- Author
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Zhou, Jun, Li, Xia, Ma, Xiaoming, Sheng, Wenlong, and Lang, Xianjun
- Subjects
- *
PHOTOCATALYSTS , *PHOTOCATALYSIS , *NANOTUBES , *CHARGE exchange , *SECONDARY amines , *OXIDATION - Abstract
The selective aerobic oxidation of benzylamine by cooperative ARS-TNTs photocatalysis with TEMPO+BF 4 −. [Display omitted] • Anatase TNTs deliver outstanding photocatalytic activity. • ARS binds with TNTs through surface hydroxyl sites. • Electron transfer occurs smoothly between ARS-TNTs and TEMPO+BF 4 −. • With increased polarity, TEMPO+BF 4 − can increase cooperative photocatalysis. • Green light-driven selective aerobic oxidation of amines has been achieved. Visible light photocatalysis could offer an eco-friendly alternative for selective transformation of organic molecules. Herein, a cooperative system is created with alizarin red S (ARS), TiO 2 nanotubes (TNTs), and an extra redox mediator. The electron transfer between oxidatively quenched ARS-TNTs and the redox mediator is the key to secure cooperative photocatalysis. The selective aerobic oxidation of primary and secondary amines was constructed by cooperative photocatalysis of ARS-TNTs with 2,2,6,6-tetramethylpiperidine-1-oxoammonium tetrafluoroborate (TEMPO+BF 4 −) driven by green light. Specifically, the activity of anatase TNTs could reach about 2.2 times that of the P25 (Aeroxide P25) TiO 2 precursor. Due to increased polarity, TEMPO+BF 4 − serves more efficiently for electron transfer than (2,2,6,6-tetramethylpiperidin-1-yl)oxy (TEMPO), conferring above 1.5 times of activity that of TEMPO for the selective aerobic conversion of amines. This work features the potential of designing redox mediators in amplifying cooperative photocatalysis driven by visible light. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
36. Eddy current thermal effect based on magnesium microrods for combined tumor therapy.
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Yang, Xiaoyuan, Yang, Nailin, Zhang, Lei, Zhao, Dongxu, Lei, Huali, Cheng, Shuning, Ge, Jun, Ma, Xiaoming, Ni, Caifang, Liu, Zhuang, and Cheng, Liang
- Subjects
- *
MAGNETOTHERAPY , *EDDIES , *MAGNESIUM , *THERMOTHERAPY , *LIVER tumors - Abstract
• (Mg@DOX) microrods were prepared by electrospinning technology for the combined MHT & chemotherapy under AMF. • Mg@DOX microrods exhibited good magnetothermal conversion efficiency and excellent penetration depth. • The controlled release of DOX could be realized under the eddy thermal effect. • Mg@DOX microrods with unique eddy thermal effects could achieve deep-seated tumor therapy through combined therapy. Magnetic hyperthermia therapy (MHT) is a minimally invasive cancer treatment to ablate tumors under an alternating magnetic field (AMF), possessing advantages including excellent tissue penetration, tumor-targeted selectivity, and negligible damage to the normal tissues. However, the low magnetothermal conversion efficiency (MTCE) of the magnetocaloric nanoagents limits its application. Herein, magnesium @ doxorubicin (Mg@DOX) microrods were prepared by electrospinning technology for the combined MHT and chemotherapy under AMF. The constructed Mg@DOX rods exhibited apparent magnetic field intensity-dependent temperature rise under AMF, stemming from the eddy thermal effect with good thermal stability, outstanding MTCE, and excellent penetration depth. Meanwhile, the DOX release increased with the prolonged magnetothermal time and the decreased pH value, thus realizing combined therapy. In vitro experiments showed that Mg@DOX microrods had an obviously cell-killing ability on account of the MHT and accelerated DOX release. In vivo experiments were further performed by implanting Mg@DOX microrods into 4T1 mouse breast tumors and the deep-seated rat liver tumors, showing excellent combined therapeutic effects. Moreover, Mg@DOX microrods showed excellent biocompatibility and displayed insignificant toxicity to the implanted animals. Our work highlighted the effective combined therapy of the unique eddy thermal effect of Mg@DOX microrods. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
37. Heterogeneous photo-electro-Fenton degradation of tetracycline through nitrogen/oxygen self-doped porous biochar supported CuFeO2 multifunctional cathode catalyst under visible light.
- Author
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Xin, Shuaishuai, Huo, Siyue, Xin, Yanjun, Gao, Mengchun, Wang, Yanhao, Liu, Wenjie, Zhang, Chunlei, and Ma, Xiaoming
- Subjects
- *
TETRACYCLINE , *TETRACYCLINES , *VISIBLE spectra , *BIOCHAR , *CATHODES , *SILVER phosphates , *CATALYSTS , *INTERSTITIAL hydrogen generation - Abstract
The nitrogen/oxygen self-doped porous biochar (NO/PBC) and NO/PBC supported CuFeO 2 (CuFeO 2 -NO/PBC) were prepared and mixed as cathode catalysts for gas diffusion electrode (GDE) fabrication. The fabricated GDE could realize H 2 O 2 electrogeneration and activate H 2 O 2 to produce active radicals through NO/PBC and CuFeO 2 , respectively. The GDE with the NO/PBC to CuFeO 2 -NO/PBC ratio of 1:1 (CuFeO 2 -NO/PBC-GDE-1.0) possessed more superior catalytic performance for tetracycline degradation in heterogeneous photo-electro-Fenton (hetero-PEF) system under visible light than other as-fabricated GDEs. The •OH was primary species, and •O 2 - was auxiliary species for hetero-PEF degradation of tetracycline through CuFeO 2 -NO/PBC-GDE-1.0. The electron on the cathode surface and photoinduced electron could accelerate Fe3+/ Fe2+ and Cu2+/ Cu+ redox cycle, promoting •OH/•O 2 - production and tetracycline degradation. The toxicity estimation of tetracycline and intermediates, TOC removal and Escherichia coli growth inhibition confirmed that the remission of overall toxicity, the inhibition of antibacterial property and the mineralization of tetracycline were achieved in hetero-PEF system. [Display omitted] • CuFeO 2 -NO/PBC was prepared by hydrothermal method using NO/PBC as a support. • CuFeO 2 -NO/PBC-GDE-1.0 was fabricated for the hetero-PEF degradation of tetracycline. • CuFeO 2 -NO/PBC-GDE-1.0 could generate and activate H 2 O 2 to produce active radicals • Cathode electron and photoinduced electron promoted regeneration of Fe2+ and Cu+. • Hetero-PEF system relieved the toxicity and antibacterial property of tetracycline. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
38. Copy number variation (CNV) of the AHR gene in the Ashidan yak and its association with growth traits.
- Author
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Dai, Rongfeng, Huang, Chun, Wu, Xiaoyun, Ma, Xiaoming, Chu, Min, Bao, Pengjia, Pei, Jie, Guo, Xian, Yan, Ping, and Liang, Chunnian
- Subjects
- *
YAK , *GENE expression profiling , *REGULATOR genes , *SINGLE nucleotide polymorphisms , *BIOLOGICAL evolution - Abstract
• The CNV of the AHR gene was verified by the real-time quantitative PCR (qRT-PCR). • Copy number variations have greater effects on animal performance than single nucleotide polymorphisms. • AHR gene may be used as a new molecular marker in yak breeding. Copy number variation (CNV) is a principal genomic structure variation affecting the gene expression through the dose-effect and change of gene regulatory region. It plays an important role in regulating the various complex traits of vertebrates. The aromatic hydrocarbon receptor (AHR) is a member of ligand-dependent transcription factors which belong to the alkaline helix-loop-helix PASS family. It is used as a conservative environmental sensor during biological evolution. This study, tracked the growth data (body weight, withers height, body length, chest girth) of 332 yaks in four stages (6, 12, 18, and 30 months) were tracked. The CNV of the yaks was analyzed using real-time quantitative PCR, and the correlation between CNV of AHR and yak growth traits was analyzed using the SPSS and R software. The AHR gene expression profiles were assessed in different tissues of the 18-month-old yak. The statistical analysis indicated the AHR -CNV of the Ashidan yak to significantly correlate with the body length (P < 0.05), and was found to be correlated with the withers height at 18 months old (P < 0.01) with extreme significance. To sum up, this study for the first time discussed the relationship between AHR -CNV and the growth traits of the Ashidan yak. The results indicated that the AHR gene might become a new molecular marker in the breeding yak. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
39. Coupling nitrogen/oxygen self-doped biomass porous carbon cathode catalyst with CuFeO2/biochar particle catalyst for the heterogeneous visible-light driven photo-electro-Fenton degradation of tetracycline.
- Author
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Xin, Shuaishuai, Huo, Siyue, Zhang, Chunlei, Ma, Xiaoming, Liu, Wenjie, Xin, Yanjun, and Gao, Mengchun
- Subjects
- *
HETEROGENEOUS catalysts , *TETRACYCLINE , *TETRACYCLINES , *BIOCHAR , *ELECTROSTATIC induction , *CATALYSTS , *SILVER phosphates - Abstract
The heterogeneous visible-light driven photo-electro-Fenton (H-VL-PEF) system was built to degrade tetracycline using nitrogen/oxygen biomass self-doped porous carbon (NO/PC) cathode catalyst coupling CuFeO 2 /biochar particle catalyst. The NO/PC possessed the H 2 O 2 selectivity of 85.3%, and the gas diffusion electrode with NO/PC as cathode catalyst (NO/PC-GDE) exhibited fine H 2 O 2 electrogeneration performance and stability. The generated electrons on CuFeO 2 /biochar by electrostatic induction and light excitation promoted Fe3+/ Fe2+ and Cu2+/ Cu+ redox cycle, which was beneficial for HO•/O 2 •- formation and tetracycline degradation. The introduction of CuFeO 2 /biochar catalyst and visible light could reduce energy consumption and promote tetracycline mineralization. The CuFeO 2 /biochar loads, initial pH and current density of H-VL-PEF system were optimized. The degradation pathway of tetracycline was speculated based on the intermediates identified by HPLC/MS. Escherichia coli growth inhibition experiments and toxicity prediction analysis demonstrated that the comprehensive toxicity of tetracycline was alleviated due to the catalytic degradation of H-VL-PEF system. [Display omitted] • Heterogeneous visible-light driven photo-electro-Fenton (H-VL-PEF) system was built. • Nitrogen/oxygen biomass self-doped porous carbon (NO/PC) had fine H 2 O 2 selectivity. • Cathode with NO/PC as electrocatalyst had good stability for H 2 O 2 generation. • H-VL-PEF system promoted regeneration of Fe2+ and Cu+ on CuFeO 2 /biochar catalyst. • The toxicity was alleviated after tetracycline was degraded in H-VL-PEF system. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
40. Podofilox suppresses gastric cancer cell proliferation by regulating cell cycle arrest and the c-Myc/ATG10 axis.
- Author
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An, Juan, Liu, Yan, Duo, Shenglan, Ma, Xiaoming, An, Ling, Yan, Yunfei, Ji, Dongde, Yan, Yupeng, Cheng, Qiliang, and Su, Zhanhai
- Subjects
- *
CANCER cell proliferation , *CELL cycle , *CELL proliferation , *STOMACH cancer , *INHIBITION of cellular proliferation , *SMALL interfering RNA - Abstract
Gastric cancer (GC) is a malignancy for which effective therapeutic drugs are limited. Podofilox exhibits antitumor effects in various types of cancer; however, whether it may inhibit GC growth remains unknown. The aim of the present study was to investigate the role of podofilox in GC. Cell Counting Kit-8, colony formation and cell cycle assays were used to detect the role of podofilox on cellular proliferation and the cell cycle, respectively. A microarray was used to detect the transcriptional changes induced by podofilox in GC cells. The results of the present study demonstrated that podofilox inhibited GC cell proliferation and colony formation. The half maximal inhibitory concentration of podofilox in AGS and HGC-27 cells was 2.327 and 1.981 nM, respectively. In addition, treatment with podofilox induced G0/G1 cell cycle arrest. Molecular analysis based on microarray data demonstrated that podofilox altered the expression levels of genes involved in the cell cycle, c-Myc and p53 signaling. Autophagy-related 10 (ATG10), which was highly expressed in GC tissues, was also downregulated by podofilox, as demonstrated by the results of the microarray analysis and immunoblotting. To determine the involvement of ATG10 in GC, ATG10 was knocked down in GC cells by small interfering RNA, which suppressed the proliferation and colony formation of GC cells compared with those observed in the control-transfected cells. Taken together, the results of the present study suggested that podofilox may inhibit GC cell proliferation by preventing the cell cycle progression and regulating the c-Myc/ATG10 signaling pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
41. Hollow hierarchically porous La2O3 with controllable multishells: A high-performance adsorbent for phosphate removal.
- Author
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Chang, Yi, Wei, Zipeng, Chang, Xiang, Ma, Guanglei, Meng, Lili, Liu, Tingting, Yang, Lin, Guo, Yuming, and Ma, Xiaoming
- Subjects
- *
PHOSPHATES , *ADSORPTION capacity , *SURFACE area , *LANTHANUM oxide , *POROSITY - Abstract
Multi-shelled nanomaterials, especially with hierarchically porous structures, are very promising for a variety of potential applications. Herein, the multi-shelled hollow La 2 O 3 nanospheres (MHLN) with high specific surface areas and numerous active sites are first fabricated via a facile and universal synthetic strategy characterized by step-by-step thermal removal of templates. The shell numbers can be easily controlled by adjusting the precursor amount adsorbed into the template. The formation mechanism of the MHLN reveals that the hierarchical assembly of the La 2 O 3 nanoparticles generates the smaller mesoporous of the shells, which providing a complicated but ordered three dimensional network for the MHLN. Notably, the combination of the multi-shelled hollow structure and the special hierarchical pores endows La 2 O 3 nanospheres not only with a large specific surface area but also with good porosity and permeability. Further functional investigation shows that MHLN exhibit a higher removal capacity (192.68 mg P g−1) for phosphate than the reported previously, a fast sorption and exhibit a good stability in wide pH range and excellent reusability. [Display omitted] • Hierarchical porous La 2 O 3 hollow nanospheres with multishells are tunably obtained. • The materials have the unique hollow multi-shell and hierarchical porous structure. • The synthetic strategy is simple and universal. • The La 2 O 3 nanospheres exhibit a high phosphate adsorption capacity and reusability. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
42. Catalytic activation of peroxydisulfate by alfalfa-derived nitrogen self-doped porous carbon supported CuFeO2 for nimesulide degradation: Performance, mechanism and DFT calculation.
- Author
-
Xin, Shuaishuai, Ma, Bingrui, Zhang, Chunlei, Ma, Xiaoming, Xu, Peng, Zhang, Guangshan, Gao, Mengchun, and Xin, Yanjun
- Subjects
- *
NIMESULIDE , *NITROGEN , *HIGH performance liquid chromatography , *CARBON , *SURFACE area , *RAW materials - Abstract
[Display omitted] • CuFeO2/NPC composites were prepared at no nitrogen sources and reductant. • CuFeO2/NPC can effectively activate PDS to degrade nimesulide. • The OH, SO4- and 1O2 were the main reactive species for nimesulide degradation. • Mechanism of PDS activated by CuFeO2/NPC was clarified in detail. • Degradation pathway was proposed combined with HPLC/MS spectra and DFT calculation. The nitrogen self-doped porous carbons (NPCs) were prepared using alfalfa as raw material by pyrolysis method without additional nitrogen sources at various pyrolysis temperatures. The NPC supported CuFeO 2 (CuFeO 2 /NPC) composites with large BET surface area (113.18 - 517.56 m2 g−1) were successfully prepared by hydrothermal method without reductant. The NPC prepared at 600 °C (NPC-600) supported CuFeO 2 (CuFeO 2 /NPC-600) possessed the highest catalytic performance for activating peroxydisulfate (PDS) to degrade nimesulide compared to other CuFeO 2 /NPC composites. The OH, SO 4 - and 1O 2 were the main reactive species for nimesulide degradation. The oxygen-containing functional groups, pyridinic N, graphitic N, and Fe3+/Fe2+ and Cu2+/Cu+ redox cycles were demonstrated to be extremely important in activating PDS to produce reactive species. The degradation pathway of nimesulide was proposed combined with the HPLC/MS spectra and DFT calculation, and the overall toxicity of nimesulide were effectively alleviated in CuFeO 2 /NPC-600+PDS system according to toxicity prediction. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
43. Hollow hierarchically porous La2O3 with controllable multishells: A high-performance adsorbent for phosphate removal.
- Author
-
Chang, Yi, Wei, Zipeng, Chang, Xiang, Ma, Guanglei, Meng, Lili, Liu, Tingting, Yang, Lin, Guo, Yuming, and Ma, Xiaoming
- Subjects
- *
ADSORPTION capacity , *PHOSPHATES , *SURFACE area , *LANTHANUM oxide , *POROSITY - Abstract
Multi-shelled nanomaterials, especially with hierarchically porous structures, are very promising for a variety of potential applications. Herein, the multi-shelled hollow La 2 O 3 nanospheres (MHLN) with high specific surface areas and numerous active sites are first fabricated via a facile and universal synthetic strategy characterized by step-by-step thermal removal of templates. The shell numbers can be easily controlled by adjusting the precursor amount adsorbed into the template. The formation mechanism of the MHLN reveals that the hierarchical assembly of the La 2 O 3 nanoparticles generates the smaller mesoporous of the shells, which providing a complicated but ordered three dimensional network for the MHLN. Notably, the combination of the multi-shelled hollow structure and the special hierarchical pores endows La 2 O 3 nanospheres not only with a large specific surface area but also with good porosity and permeability. Further functional investigation shows that MHLN exhibit a higher removal capacity (192.68 mg P g−1) for phosphate than the reported previously, a fast sorption and exhibit a good stability in wide pH range and excellent reusability. [Display omitted] • Hierarchical porous La 2 O 3 hollow nanospheres with multishells are tunably obtained. • The materials have the unique hollow multi-shell and hierarchical porous structure. • The synthetic strategy is simple and universal. • The La 2 O 3 nanospheres exhibit a high phosphate adsorption capacity and reusability. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
Catalog
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