29 results on '"Yong Wang"'
Search Results
2. Dealuminated Beta zeolite reverses Ostwald ripening for durable copper nanoparticle catalysts.
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Lujie Liu, Jiaye Lu, Yahui Yang, Ruettinger, Wolfgang, Xinhua Gao, Ming Wang, Hao Lou, Zhandong Wang, Yifeng Liu, Xin Tao, Lina Li, Yong Wang, Hangjie Li, Hang Zhou, Chengtao Wang, Qingsong Luo, Huixin Wu, Kaidi Zhang, Jiabi Ma, and Xiaoming Cao
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- 2024
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3. The oscillating Fischer-Tropsch reaction.
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Rui Zhang, Yong Wang, Gaspard, Pierre, and Kruse, Norbert
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OSCILLATING chemical reactions , *CARBON monoxide , *ATMOSPHERIC pressure , *PARTIAL pressure , *CERIUM , *OSCILLATIONS - Abstract
The mechanistic steps that underlie the formation of higher hydrocarbons in catalytic carbon monoxide (CO) hydrogenation at atmospheric pressure over cobalt-based catalysts (Fischer-Tropsch synthesis) have remained poorly understood. We reveal nonisothermal rate-and-selectivity oscillations that are self-sustained over extended periods of time (’24 hours) for a cobalt/cerium oxide catalyst with an atomic ratio of cobalt to cerium of 2:1 (Co2Ce1) at 220°C and equal partial pressures of the reactants. A microkinetic mechanism was used to generate rate-and-selectivity oscillations through forced temperature oscillations. Experimental and theoretical oscillations were in good agreement over an extended range of reactant pressure ratios. Additionally, phase portraits for hydrocarbon production were constructed that support the thermokinetic origin of our rate-and-selectivity oscillations. [ABSTRACT FROM AUTHOR]
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- 2023
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4. Meteorin-like promotes heart repair through endothelial KIT receptor tyrosine kinase.
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Reboll, Marc R., Klede, Stefanie, Taft, Manuel H., Chen-Leng Cai, Field, Loren J., Lavine, Kory J., Koenig, Andrew L., Fleischauer, Jenni, Meyer, Johann, Schambach, Axel, Niessen, Hans W., Kosanke, Maike, van den Heuvel, Joop, Pich, Andreas, Bauersachs, Johann, Xuekun Wu, Linqun Zheng, Yong Wang, Korf-Klingebiel, Mortimer, and Polten, Felix
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- 2022
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5. DNA methyltransferase 3B deficiency unveils a new pathological mechanism of pulmonary hypertension
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Xin Jiang, Yi Yan, Jiwang Chen, Shan-Shan Guo, Shuyang Zhang, Xi-Qi Xu, Su-Qi Li, Yang-Yang He, Zhi-Cheng Jing, Ru-Jiao Zhang, Jue Ye, Dan Lu, Xue Zhang, Tian-Yu Lian, Kai Sun, Yong Wang, Jun-Han Zhao, Xu Zhang, and Lian-Feng Zhang
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Hypertension, Pulmonary ,DNMT3B ,Becaplermin ,Diseases and Disorders ,030204 cardiovascular system & hematology ,Vascular Remodeling ,Pathogenesis ,Rats, Sprague-Dawley ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Mediator ,Right ventricular hypertrophy ,medicine.artery ,medicine ,Animals ,Humans ,Epigenetics ,Health and Medicine ,DNA (Cytosine-5-)-Methyltransferases ,Hypoxia ,Research Articles ,Cells, Cultured ,030304 developmental biology ,Cell Proliferation ,0303 health sciences ,Multidisciplinary ,business.industry ,SciAdv r-articles ,medicine.disease ,Pulmonary hypertension ,Rats ,Disease Models, Animal ,embryonic structures ,DNA methylation ,Pulmonary artery ,Cancer research ,business ,Research Article - Abstract
DNA methyltransferase 3B is identified as a protective target against pulmonary vascular remodeling., DNA methylation plays critical roles in vascular pathology of pulmonary hypertension (PH). The underlying mechanism, however, remains undetermined. Here, we demonstrate that global DNA methylation was elevated in the lungs of PH rat models after monocrotaline administration or hypobaric hypoxia exposure. We showed that DNA methyltransferase 3B (DNMT3B) was up-regulated in both PH patients and rodent models. Furthermore, Dnmt3b−/− rats exhibited more severe pulmonary vascular remodeling. Consistently, inhibition of DNMT3B promoted proliferation/migration of pulmonary artery smooth muscle cells (PASMCs) in response to platelet-derived growth factor–BB (PDGF-BB). In contrast, overexpressing DNMT3B in PASMCs attenuated PDGF-BB–induced proliferation/migration and ameliorated hypoxia-mediated PH and right ventricular hypertrophy in mice. We also showed that DNMT3B transcriptionally regulated inflammatory pathways. Our results reveal that DNMT3B is a previously undefined mediator in the pathogenesis of PH, which couples epigenetic regulations with vascular remodeling and represents a therapeutic target to tackle PH.
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- 2020
6. Retinoid X receptor alpha is a spatiotemporally predominant therapeutic target for anthracycline-induced cardiotoxicity
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Alexey V. Dvornikov, Yong Wang, Qi Qiu, Xueying Lin, Tzung K. Hsiai, Xiaolei Xu, Yonghe Ding, Maengjo Kim, Zhang Hong, Yue Yu, Nadine Norton, Joerg Herrmann, Matthew R. Lowerison, Stephen C. Ekker, Xiao Ma, Ping Zhu, and Zheng Wang
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Cardiotonic Agents ,Mutant ,030204 cardiovascular system & hematology ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,In vivo ,law ,Neoplasms ,Genetics ,Medicine ,Animals ,Humans ,Health and Medicine ,Isotretinoin ,Zebrafish ,Research Articles ,030304 developmental biology ,Bexarotene ,0303 health sciences ,Cardiotoxicity ,Multidisciplinary ,Retinoid X Receptor alpha ,Retinoid X receptor alpha ,biology ,business.industry ,Myocardium ,SciAdv r-articles ,Endothelial Cells ,Heart ,biology.organism_classification ,3. Good health ,Disease Models, Animal ,Cancer research ,Zonula Occludens-1 Protein ,Suppressor ,business ,Pericardium ,medicine.drug ,Research Article - Abstract
RXRA is an endothelial- and early stage–predominant therapeutic target for treating anthracycline-induced cardiotoxicity., To uncover the genetic basis of anthracycline-induced cardiotoxicity (AIC), we recently established a genetic suppressor screening strategy in zebrafish. Here, we report the molecular and cellular nature of GBT0419, a salutary modifier mutant that affects retinoid x receptor alpha a (rxraa). We showed that endothelial, but not myocardial or epicardial, RXRA activation confers AIC protection. We then identified isotretinoin and bexarotene, two FDA-approved RXRA agonists, which exert cardioprotective effects. The therapeutic effects of these drugs only occur when administered during early, but not late, phase of AIC or as pretreatment. Mechanistically, these spatially- and temporally-predominant benefits of RXRA activation can be ascribed to repair of damaged endothelial cell-barrier via regulating tight-junction protein Zonula occludens-1. Together, our study provides the first in vivo genetic evidence supporting RXRA as the therapeutic target for AIC, and uncovers a previously unrecognized spatiotemporally-predominant mechanism that shall inform future translational efforts.
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- 2020
7. How Pore Hydrophilicity Influences Water Permeability?
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Yang Song, Wei Zhou, Yong Wang, Mingjie Wei, Xin Zhang, and Fang Xu
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Pressure drop ,Multidisciplinary ,Materials science ,Science ,Clean water ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Membrane technology ,Permeability (earth sciences) ,Membrane ,Chemical engineering ,Molecule ,lcsh:Q ,Wetting ,0210 nano-technology ,lcsh:Science ,Research Article ,Nanosheet - Abstract
Membrane separation is playing increasingly important role in providing clean water. Simulations predict that membrane pores with strong hydrophobicity produce ultrahigh water permeability as a result of low friction. However, experiments demonstrate that hydrophilic pores favor higher permeability. Herein we simulate water molecules transporting through interlayers of two-dimensional nanosheets with various hydrophilicities using nonequilibrium molecular dynamics. We reveal that there is a threshold pressure drop (Δ P T ), exceeding which stable water permeability appears. Strongly hydrophobic pores exhibit extremely high Δ P T , prohibiting the achievement of ultrahigh water permeability under the experimentally accessible pressures. Under pressures < Δ P T , water flows in hydrophobic pores in a running-stop mode because of alternative wetting and nonwetting, thus leading to significantly reduced permeability. We discover that hydrophilic modification to one surface of the nanosheet can remarkably reduce Δ P T by > 99%, indicating a promising strategy to experimentally realize ultrafast membranes.
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- 2019
8. Selective Electrochemical Reduction of Nitrogen to Ammonia by Adjusting the Three-Phase Interface
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Shanjun Mao, Yong Wang, Ruxue Fan, Jiadong Chen, Yuzhuo Chen, Haiyan Wang, and Zhe Wang
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Multidisciplinary ,Aqueous solution ,Materials science ,Science ,02 engineering and technology ,Electrolyte ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Electrochemistry ,01 natural sciences ,Redox ,0104 chemical sciences ,Catalysis ,Ammonia ,chemistry.chemical_compound ,Adsorption ,Chemical engineering ,chemistry ,0210 nano-technology ,Selectivity ,Research Article - Abstract
The electrochemical nitrogen reduction reaction (NRR) provides a sustainable and alternative avenue to the Haber-Bosch process for ammonia (NH 3 ) synthesis. Despite the great efforts made on catalysts and electrolytes, unfortunately, current NRR suffers from low selectivity due to the overwhelming competition with the hydrogen evolution reaction (HER). Here, we present an adjusted three-phase interface to enhance nitrogen (N 2 ) coverage on a catalyst surface and achieve a record-high Faradic efficiency (FE) up to 97% in aqueous solution. The almost entirely suppressed HER process combined with the enhanced NRR activity, benefiting from the efficient three-interface contact line, is responsible for the excellent selectivity toward NH 3 , as evidenced by the theoretical and experimental results. Our strategy also demonstrates the applicability to other catalysts that feature strong H adsorption ability, to boost the FE for NH 3 synthesis above 90% and to improve the NRR activity by engineering the catalysts.
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- 2019
9. Structural basis for sugar perception by Drosophila gustatory receptors.
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Demin Ma, Meiqin Hu, Xiaotong Yang, Qiang Liu, Fan Ye, Weijie Cai, Yong Wang, Ximing Xu, Shenghai Chang, Ruiying Wang, Wei Yang, Sheng Ye, Nannan Su, Minrui Fan, Haoxing Xu, and Jiangtao Guo
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- 2024
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10. In situ stiffness manipulation using elegant curved origami.
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Zirui Zhai, Yong Wang, Ken Lin, Lingling Wu, and Hanqing Jiang
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METAMATERIALS , *STIFFNESS (Engineering) , *ORIGAMI , *MECHANICAL buckling , *UNIT cell , *FINITE element method - Abstract
The article focuses on the study of the In situ stiffness manipulation using elegant curved origami. It mentions that the capability of stiffness manipulation for materials and structures is essential for tuning motion, saving energy, and delivering high power; and high-efficiency in situ stiffness manipulation has not yet been successfully achieved despite many studies from different perspectives.
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- 2020
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11. Visualizing H2O molecules reacting at TiO2 active sites with transmission electron microscopy.
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Wentao Yuan, Beien Zhu, Xiao-Yan Li, Hansen, Thomas W., Yang Ou, Ke Fang, Hangsheng Yang, Ze Zhang, Wagner, Jakob B., Yi Gao, and Yong Wang
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- 2020
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12. Activation of surface lattice oxygen in single-atom Pt/CeO2 for low-temperature CO oxidation.
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Lei Nie, Donghai Mei, Haifeng Xiong, Bo Peng, Zhibo Ren, Pereira Hernandez, Xavier Isidro, DeLaRiva, Andrew, Meng Wang, Engelhard, Mark H., Kovarik, Libor, DatyeDepartment of Chemical and Biological Engineering andCenter for Micro-Engineered Materials, University of NewMexico, Albuquerque, NM 87131, USA.†Corresponding author. Email: datye@unm.edu, Abhaya K., and Yong Wang
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- 2017
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13. Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9.
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Dong Niu, Hong-Jiang Wei, Lin Lin, George, Haydy, Tao Wang, I-Hsiu Lee, Hong-Ye Zhao, Yong Wang, Yinan Kan, Shrock, Ellen, Lesha, Emal, Gang Wang, Yonglun Luo, Yubo Qing, Deling Jiao, Heng Zhao, Xiaoyang Zhou, Shouqi Wang, Hong Wei, and Güell, Marc
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- 2017
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14. Noninvasive Electroanatomic Mapping of Human Ventricular Arrhythmias with Electrocardiographic Imaging (ECGI).
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Yong Wang, Cuculich, Phillip S., Junjie Zhang, Desouza, Kavit A., Vijayakumar, Ramya, Jane Chen, Faddis, Mitchell N., Lindsay, Bruce D., Smith, Timothy W., and Rudy, Yoram
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- 2011
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15. DNMT1 Stability Is Regulated by Proteins Coordinating Deubiquitination and Acetylation-Driven Ubiquitination.
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Zhanwen Du, Jing Song, Yong Wang, Yiqing Zhao, Guda, Kishore, Shuming Yang, Hung-Ying Kao, Yan Xu, Willis, Joseph, Markowitz, Sanford D., Sedwick, David, Ewing, Robert M., and Zhenghe Wang
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- 2010
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16. Nuclear pyruvate dehydrogenase complex regulates histone acetylation and transcriptional regulation in the ethylene response.
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Zhengyao Shao, Liangqiao Bian, Ahmadi, Shyon K., Daniel, Tyler J., Belmonte, Miguel A., Burns, Jackson G., Kotla, Prashanth, Yang Bi, Zhouxin Shen, Shou-Ling Xu, Zhi-Yong Wang, Briggs, Steven P., and Hong Qiao
- Abstract
Ethylene plays its essential roles in plant development, growth, and defense responses by controlling the transcriptional reprograming, in which EIN2-C-directed regulation of histone acetylation is the first key step for chromatin to perceive ethylene signaling. But how the nuclear acetyl coenzyme A (acetyl CoA) is produced to ensure the ethylene-mediated histone acetylation is unknown. Here we report that ethylene triggers the accumulation of the pyruvate dehydrogenase complex (PDC) in the nucleus to synthesize nuclear acetyl CoA to regulate ethylene response. PDC is identified as an EIN2-C nuclear partner, and ethylene triggers its nuclear accumulation. Mutations in PDC lead to an ethylene hyposensitivity that results from the reduction of histone acetylation and transcription activation. Enzymatically active nuclear PDC synthesizes nuclear acetyl CoA for EIN2-C-directed histone acetylation and transcription regulation. These findings uncover a mechanism by which PDC-EIN2 converges the mitochondrial enzyme-mediated nuclear acetyl CoA synthesis with epigenetic and transcriptional regulation for plant hormone response. [ABSTRACT FROM AUTHOR]
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- 2024
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17. ROBO1 deficiency impairs HSPC homeostasis and erythropoiesis via CDC42 and predicts poor prognosis in MDS.
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Jia-Cheng Jin, Bing-Yi Chen, Chu-Han Deng, Jia-Nan Chen, Feng Xu, Ying Tao, Cheng-Long Hu, Chun-Hui Xu, Bin-He Chang, Yong Wang, Ming-Yue Fei, Ping Liu, Peng-Cheng Yu, Zi-Juan Li, Xi-Ya Li, Shu-Bei Chen, Yi-Lun Jiang, Xin-Chi Chen, Li-Juan Zong, and Jia-Ying Zhang
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CELL cycle proteins , *ERYTHROPOIESIS , *HEMATOPOIESIS , *HOMEOSTASIS , *HEMATOPOIETIC stem cells , *HEMATOLOGIC malignancies , *MYELODYSPLASTIC syndromes - Abstract
Myelodysplastic syndrome (MDS) is a group of clonal hematopoietic neoplasms originating from hematopoietic stem progenitor cells (HSPCs). We previously identified frequent roundabout guidance receptor 1 (ROBO1) mutations in patients with MDS, while the exact role of ROBO1 in hematopoiesis remains poorly delineated. Here, we report that ROBO1 deficiency confers MDS-like disease with anemia and multilineage dysplasia in mice and predicts poor prognosis in patients with MDS. More specifically, Robo1 deficiency impairs HSPC homeostasis and disrupts HSPC pool, especially the reduction of megakaryocyte erythroid progenitors, which causes a blockage in the early stages of erythropoiesis in mice. Mechanistically, transcriptional profiling indicates that Cdc42, a member of the Rho-guanosine triphosphatase family, acts as a downstream target gene for Robo1 in HSPCs. Overexpression of Cdc42 partially restores the self-renewal and erythropoiesis of HSPCs in Robo1-deficient mice. Collectively, our result implicates the essential role of ROBO1 in maintaining HSPC homeostasis and erythropoiesis via CDC42. [ABSTRACT FROM AUTHOR]
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- 2023
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18. An Aboriginal Australian Genome Reveals Separate Human Dispersals into Asia.
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Rasmussen, Morten, Xiaosen Guo, Yong Wang, Lohmueller, Kirk E., Rasmussen, Simon, Albrechtsen, Anders, Skotte, Line, Lindgreen, Stinus, Metspalu, Mait, Jombart, Thibaut, Kivisild, Toomas, Weiwei Zhai, Eriksson, Anders, Manica, Andrea, Orlando, Ludovic, De La Vega, Francisco M., Tridico, Silvana, Metspalu, Ene, Nielsen, Kasper, and Ávila-Arcos, María C.
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FOSSIL DNA , *ABORIGINAL Australians , *NUCLEOTIDE sequence , *HUMAN migrations , *HUMAN population genetics , *GENETICS , *BIOLOGICAL evolution - Abstract
We present an Aboriginal Australian genomic sequence obtained from a 100-year-old lock of hair donated by an Aboriginal man from southern Western Australia in the early 20th century. We detect no evidence of European admixture and estimate contamination levels to be below 0.5%. We show that Aboriginal Australians are descendants of an early human dispersal into eastern Asia, possibly 62,000 to 75,000 years ago. This dispersal is separate from the one that gave rise to modern Asians 25,000 to 38,000 years ago. We also find evidence of gene flow between populations of the two dispersal waves prior to the divergence of Native Americans from modern Asian ancestors. Our findings support the hypothesis that present-day Aboriginal Australians descend from the earliest humans to occupy Australia, likely representing one of the oldest continuous populations outside Africa. [ABSTRACT FROM AUTHOR]
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- 2011
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19. Order and disorder--An integrative structure of the full-length human growth hormone receptor.
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Kassem, Noah, Araya-Secchi, Raul, Bugge, Katrine, Barclay, Abigail, Steinocher, Helena, Khondker, Adree, Yong Wang, Lenard, Aneta J., Bürck, Jochen, Sahin, Cagla, Ulrich, Anne S., Landreh, Michael, Pedersen, Martin Cramer, Rheinstädter, Maikel C., Pedersen, Per Amstrup, Lindorff-Larsen, Kresten, Arleth, Lise, and Kragelund, Birthe B.
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SOMATOTROPIN receptors , *HUMAN growth hormone , *SMALL-angle X-ray scattering , *MEMBRANE proteins , *MOLECULAR dynamics , *MEMBRANE lipids , *CYTOKINE receptors - Abstract
Because of its small size (70 kilodalton) and large content of structural disorder (>50%), the human growth hormone receptor (hGHR) falls between the cracks of conventional high-resolution structural biology methods. Here, we study the structure of the full-length hGHR in nanodiscs with small-angle x-ray scattering (SAXS) as the foundation. We develop an approach that combines SAXS, x-ray diffraction, and NMR spectroscopy data obtained on individual domains and integrate these through molecular dynamics simulations to interpret SAXS data on the full-length hGHR in nanodiscs. The hGHR domains reorient freely, resulting in a broad structural ensemble, emphasizing the need to take an ensemble view on signaling of relevance to disease states. The structure provides the first experimental model of any full-length cytokine receptor in a lipid membrane and exemplifies how integrating experimental data from several techniques computationally may access structures of membrane proteins with long, disordered regions, a widespread phenomenon in biology. [ABSTRACT FROM AUTHOR]
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- 2021
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20. DNA methyltransferase 3B deficiency unveils a new pathological mechanism of pulmonary hypertension.
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Yi Yan, Yang-Yang He, Xin Jiang, Yong Wang, Ji-Wang Chen, Jun-Han Zhao, Jue Ye, Tian-Yu Lian, Xu Zhang, Ru-Jiao Zhang, Dan Lu, Shan-Shan Guo, Xi-Qi Xu, Kai Sun, Su-Qi Li, Lian-Feng Zhang, Xue Zhang, Shu-Yang Zhang, and Zhi-Cheng Jing
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BONE morphogenetic protein receptors , *DNA methyltransferases , *MEDICAL sciences , *PULMONARY hypertension , *VASCULAR endothelial growth factor receptors , *VASCULAR remodeling - Abstract
The article discusses the critical role of DNA methylation in the vascular pathology of pulmonary hypertension (PH). Topics include a study which showed that global DNA methylation in the lungs of PH rat models following monocrotaline administration or hypobaric hypoxia exposure, the up-regulation of DNA methyl-transferase 3B (DNMT3B), and the migration of pulmonary artery smooth muscle cells (PASMC).
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- 2020
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21. Retinoid X receptor alpha is a spatiotemporally predominant therapeutic target for anthracycline-induced cardiotoxicity.
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Xiao Ma, Ping Zhu, Yonghe Ding, Hong Zhang, Qi Qiu, Dvornikov, Alexey V., Zheng Wang, Maengjo Kim, Yong Wang, Lowerison, Matthew, Yue Yu, Norton, Nadine, Herrmann, Joerg, Ekker, Stephen C., Hsiai, Tzung K., Xueying Lin, and Xiaolei Xu
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ANTHRACYCLINES , *RETINOID X receptors , *MEDICAL sciences , *TIGHT junctions , *CARDIOTOXICITY , *RETINOIC acid receptors , *PHARMACOLOGY , *PLURIPOTENT stem cells - Abstract
The article presents a study on how retinoid X receptor alpha is a spatiotemporally predominant therapeutic target for anthracycline-induced cardiotoxicity. Topics discussed include unrecognized spatiotemporally-predominant mechanism that shall inform future translational efforts; Therapeutic strategies based on these noncardiomyocytes, and remain underexplored; and elucidate the disease mechanisms and to search for potential therapeutic targets, several genetic strategies.
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- 2020
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22. Discovery of log-periodic oscillations in ultraquantum topological materials.
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Huichao Wang, Haiwen Liu, Yanan Li, Yongjie Liu, Junfeng Wang, Jun Liu, Ji-Yan Dai, Yong Wang, Liang Li, Jiaqiang Yan, Mandrus, David, Xie, X. C., and Jian Wang
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OSCILLATIONS , *SINGLE crystals - Abstract
The article discusses the research on quantum oscillations in ultraquantum topological materials and tackles the discrete scale invariance (DSI) of single crystal.
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- 2018
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23. Thermally stable single-atom platinum-on-ceria catalysts via atom trapping.
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Jones, John, Haifeng Xiong, DeLaRiva, Andrew T., Peterson, Eric J., Hien Pham, Challa, Sivakumar R., Gongshin Qi, Se Oh, Wiebenga, Michelle H., Pereira Hernández, Xavier Isidro, Yong Wang, and Datye, Abhaya K.
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PLATINUM catalysts , *ALUMINUM catalysts , *ATOM trapping , *CATALYSIS , *MOLECULE trapping - Abstract
Catalysts based on single atoms of scarce precious metals can lead to more efficient use through enhanced reactivity and selectivity. However, single atoms on catalyst supports can be mobile and aggregate into nanoparticles when heated at elevated temperatures. High temperatures are detrimental to catalyst performance unless these mobile atoms can be trapped. We used ceria powders having similar surface areas but different exposed surface facets. When mixed with a platinum/aluminum oxide catalyst and aged in air at 800°C, the platinum transferred to the ceria and was trapped. Polyhedral ceria and nanorods were more effective than ceria cubes at anchoring the platinum. Performing synthesis at high temperatures ensures that only the most stable binding sites are occupied, yielding a sinter-resistant, atomically dispersed catalyst. [ABSTRACT FROM AUTHOR]
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- 2016
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24. A mutually assured destruction mechanism attenuates light signaling in Arabidopsis.
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Weimin Ni, Shou-Ling Xu, Tepperman, James M., Stanley, David J., Maltby, Dave A., Gross, John D., Burlingame, Alma L., Zhi-Yong Wang, and Quail, Peter H.
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ARABIDOPSIS , *CHROMOSOMAL translocation , *PHYTOCHROMES , *PLANT phosphorylation , *GENETIC regulation in plants , *EFFECT of light on plants , *HELIX-loop-helix motif genetics , *PLANTS - Abstract
After light-induced nuclear translocation, phytochrome photoreceptors interact with and induce rapid phosphorylation and degradation of basic helix-loop-helix transcription factors, such as PHYTOCHROME-INTERACTING FACTOR 3 (PIF3), to regulate gene expression. Concomitantly, this interaction triggers feedback reduction of phytochrome B (phyB) levels. Light-induced phosphorylation of PIF3 is necessary for the degradation of both proteins. We report that this PIF3 phosphorylation induces, and is necessary for, recruitment of LRB [Light-Response Bric-a-Brack/Tramtrack/Broad (BTB)] E3 ubiquitin ligases to the PIF3-phyB complex. The recruited LRBs promote concurrent polyubiqutination and degradation of both PIF3 and phyB in vivo. These data reveal a linked signal-transmission and attenuation mechanism involving mutually assured destruction of the receptor and its immediate signaling partner. [ABSTRACT FROM AUTHOR]
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- 2014
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25. Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli.
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Ajikumar, Parayil Kumaran, Wen-Hai Xiao, Tyo, Keith E. J., Yong Wang, Simeon, Fritz, Leonard, Effendi, Mucha, Oliver, Too Heng Phon, Pfeifer, Blaine, and Stephanopoulos, Gregory
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ISOPENTENOIDS , *INDUSTRIAL efficiency , *PACLITAXEL , *COST effectiveness , *ESCHERICHIA coli , *INDOLE , *METABOLISM , *CANCER treatment - Abstract
Taxol (paclitaxel) is a potent anticancer drug first isolated from the Taxus brevifolia Pacific yew tree. Currently, cost-efficient production of Taxol and its analogs remains limited. Here, we report a multivariate-modular approach to metabolic-pathway engineering that succeeded in increasing titers of taxadiene--the first committed Taxol intermediate--approximately 1 gram per liter (∼15,000-fold) in an engineered Escherichia coli strain. Our approach partitioned the taxadiene metabolic pathway into two modules: a native upstream methylerythritol-phosphate (AAEP) pathway forming isopentenyl pyrophosphate and a heterologous downstream terpenoid-forming pathway. Systematic multivariate search identified conditions that optimally balance the two pathway modules so as to maximize the taxadiene production with minimal accumulation of indole, which is an inhibitory compound found here. We also engineered the next step in Taxol biosynthesis, a P450-mediated 5a-oxidation of taxadiene to taxadien-5a-ol. More broadly, the modular pathway engineering approach helped to unlock the potential of the MEP pathway for the engineered production of terpenoid natural products. [ABSTRACT FROM AUTHOR]
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- 2010
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26. BSKs Mediate Signal Transduction from the Receptor Kinase BRI1 in Arabidopsis.
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Wenqiang Tang, Tae-Wuk Kim, Oses-Prieto, Juan A., Yu Sun, Zhiping Deng, Shengwei Zhu, Ruiju Wang, Burlingame, Alma L., and Zhi-Yong Wang
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BRASSINOSTEROIDS , *PLANT hormones , *FOCAL adhesion kinase , *GENETIC transduction , *MICROBIAL genetics , *GENETIC regulation , *PLANT development , *CELL membranes , *DEVELOPMENTAL biology - Abstract
Brassinosteroids (BRs) bind to the extracellular domain of the receptor kinase BRI1 to activate a signal transduction cascade that regulates nuclear gene expression and plant development. Many components of the BR signaling pathway have been identified and studied in detail. However, the substrate of BRI1 kinase that transduces the signal to downstream components remains unknown. Proteomic studies of plasma membrane proteins lead to the identification of three homologous BR-signaling kinases (BSK1, BSK2, and BSK3). The BSKs are phosphorylated by BRI1 in vitro and interact with BRI1 in vivo. Genetic and transgenic studies demonstrate that the BSKs represent a small family of kinases that activate BR signaling downstream of BRI1. These results demonstrate that BSKs are the substrates of BRI1 kinase that activate downstream BR signal transduction. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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27. Divergence of Transcription Factor Binding Sites Across Related Yeast Species.
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Borneman, Anthony R., Gianoulis, Tara A., Zhang, Zhengdong D., Haiyuan Yu, Rozowsky, Joel, Seringhaus, Michael R., Lu Yong Wang, Gerstein, Mark, and Snyder, Michael
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BIOLOGICAL divergence , *TRANSCRIPTION factors , *BINDING sites , *YEAST , *GENETIC regulation , *GENETIC transcription regulation , *SACCHAROMYCES , *MOLECULAR genetics , *DNA microarrays - Abstract
Characterization of interspecies differences in gene regulation is crucial for understanding the molecular basis of both phenotypic diversity and evolution. By means of chromatin immunoprecipitation and DNA microarray analysis, the divergence in the binding sites of the pseudohyphal regulators Ste12 and Tec1 was determined in the yeasts Saccharomyces cerevisiae, S. mikatae, and S. bayanus under pseudohyphal conditions. We have shown that most of these sites have diverged across these species, far exceeding the interspecies variation in orthologous genes. A group of Ste12 targets was shown to be bound only in S. mikatae and S. bayanus under pseudohyphal conditions. Many of these genes are targets of Ste12 during mating in S. cerevisiae, indicating that specialization between the two pathways has occurred in this species. Transcription factor binding sites have therefore diverged substantially faster than ortholog content. Thus, gene regulation resulting from transcription factor binding is likely to be a major cause of divergence between related species. [ABSTRACT FROM AUTHOR]
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- 2007
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28. Yersinia YopJ Acetylates and Inhibits Kinase Activation by Blocking Phosphorylation.
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Mukherjee, Sohini, Keitany, Gladys, Yan Li, Yong Wang, Ball, Haydn L., Goldsmith, Elizabeth J., and Orth, Kim
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YERSINIA , *IMMUNE response , *CHEMICAL reactions , *MITOGEN-activated protein kinases , *ACETYLCOENZYME A , *PHOSPHOTRANSFERASES , *ACYLATION , *AMINO acids , *ACETYLTRANSFERASES - Abstract
Yersinia species use a variety of type III effector proteins to target eukaryotic signaling systems. The effector YopJ inhibits mitogen-activated protein kinase (MAPK) and the nuclear factor κB (NFκB) signaling pathways used in innate immune response by preventing activation of the family of MAPK kinases (MAPKK). We show that YopJ acted as an acetyltransferase, using acetylcoenzyme A (CoA) to modify the critical serine and threonine residues in the activation loop of MAPKK6 and thereby blocking phosphorylation. The acetylation on MAPKK6 directly competed with phosphorytation, preventing activation of the modified protein. This covalent modification may be used as a general regulatory mechanism in biological signaling. [ABSTRACT FROM AUTHOR]
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- 2006
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29. Large Electrocaloric Effect in Ferroelectric Polymers Near Room Temperature.
- Author
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Neese, Bret, Chu, Baojin, Sheng-Guo Lu, Yong Wang, Furman, E., and Zhang, Q. M.
- Subjects
- *
PYROELECTRICITY , *PYROELECTRIC detectors , *FERROELECTRIC crystals , *FERROELECTRIC devices , *POLYMERS , *ELECTRIC fields , *MAXWELL equations , *ISOTHERMAL transformation diagrams , *THERMODYNAMICS - Abstract
Applying an electrical field to a polar polymer may induce a large change in the dipolar ordering, and if the associated entropy changes are large, they can be explored in cooling applications. With the use of the Maxwell relation between the pyroelectric coefficient and the electrocaloric effect (ECE), it was determined that a large ECE can be realized in the ferroelectric poly(vinylidene fluoride-trifluoroethylene) [P(VDF-TrFE)] copolymer at temperatures above the ferroetectric-paraelectric transition (above 70°C), where an isothermal entropy change of more than 55 joules per kilogram per kelvin degree and adiabatic temperature change of more than 12°C were observed. We further showed that a similar level of ECE near room temperature can be achieved by working with the relaxor ferroelectric polymer of P(VDF-TrFE-chlorofluoroethylene). [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
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