27 results on '"CANBIN LIN"'
Search Results
2. Circular RNA atlas in osteoclast differentiation with and without alendronate treatment
- Author
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Jianbiao Lin, Shaofeng Ma, Cong Zhu, Changqing Chen, Weibin Lin, Canbin Lin, Guofeng Huang, and Zhenqi Ding
- Subjects
Alendronate ,Osteoporosis ,MCSF ,RANKL ,Orthopedic surgery ,RD701-811 ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background Alendronate (AL) is the most widely used bisphosphonate in the treatment of osteoporosis (OP). However, the role of circular RNAs (circRNAs) in the treatment of OP with AL remains unclear. Methods In this study, we showed that osteoclast (OC) precursors (OPCSs) could be induced into OCs with macrophage colony-stimulating factor (MCSF) and receptor activator of nuclear factor-κB ligand (RANKL) treatment. Subsequently, the OCs were treated with AL. OC differentiation-related biomarkers including RANK, tartrate-resistant acid phosphatase (TRAP), and cathepsin K (CTSK) were analyzed with TRAP staining, quantitative real-time (qPCR), and western blotting. Differentially expressed circRNAs (DECs) were identified among the OPCS, OC, and OC + AL groups. In addition, the expression levels of 10 DECs related to OC differentiation were verified by qPCR. Results TRAP staining showed that MCSF and RANKL treatment effectively induced OPCSs to differentiate into OCs. In addition, qPCR and western blot analysis revealed that the three biomarkers of OC (RANK, TRAP, and CTSK) were expressed significantly more in the OC group than those in the OPCS group. In contrast, the mRNA and protein expression levels of these three biomarkers decreased significantly in OCs treated with AL compared with those non-treated OCs. GO analysis of the DECs in the OPCS group vs. the OC group revealed that their functions were mainly related to cell, cell part, binding, and single-organism terms. KEGG analysis of the top 20 DECs in a comparison between the OPCS and OC groups showed that genes involved in mitogen-activated protein kinase signaling were the most common. Results of functional analyses of DECs in an OC vs. OC + AL comparison were similar to those in the OPCS vs. OC comparison. Finally, qPCR showed that, in the OC + AL vs. OC group comparison, the expression levels of seven and three DECs significantly decreased and increased, respectively. Conclusions Having successfully induced OPCSs to differentiate into OCs, we showed that AL suppresses the differentiation of OPCS into OC and that 10 DECs were involved in the regulation of this process. This indicates that these DECs might be important to the treatment of OP.
- Published
- 2020
- Full Text
- View/download PDF
3. MiR-23a-3p acts as an oncogene and potential prognostic biomarker by targeting PNRC2 in RCC
- Author
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Jing Quan, Xiang Pan, Yawen Li, Yimin Hu, Lingzhi Tao, Zuwei Li, Liwen Zhao, Jingyao Wang, Hang Li, Yulin Lai, Liang Zhou, Canbin Lin, Yaoting Gui, Jing Ye, Fangting Zhang, and Yongqing Lai
- Subjects
miR-23a-3p ,PNRC2 ,Renal cell carcinoma ,Oncogene ,Prognosis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: Renal cell carcinoma (RCC) is a most common kidney malignancy, with atypical symptoms in the early stage and poor outcome in the late stage. Recently, emerging evidence revealed that some miRNAs play an essential role in the tumorigenesis and progression of RCC. Therefore, the aim of this study is that understand the detailed molecular mechanism of miR-23a-3p in RCC and identify its potential clinical value. Methods: In this study, RT-qPCR, wound scratch assay, cell proliferation assay, transwell assay and flow cytometry assay were performed to detect miR-23a-3p expression and its proliferation, migration and apoptosis in RCC. The bioinformatics analysis, RT-qPCR, western blot and luciferase reporter assay were performed to discern and examine the relationship between miR-23a-3p and its potential targets. Moreover, we analyzed the relationship between miR-23a-3p expression and clinicopathological variables or overall survival (OS) from 118 formalin-fixed paraffin-embedded RCC samples. Results: miR-23a-3p is significantly up-regulated in RCC tissue samples, RCC cell lines and the TCGA database. Upregulating miR-23a-3p enhances, while silencing miR-23a-3p suppresses cell viability, proliferation and mobility in ACHN and 786-O cell lines. Besides, overexpression of miR-23a-3p inhibits the cell apoptosis. Then our study further reveals that miR-23a-3p regulates tumorigenesis by targeting Proline-Rich Nuclear Receptor Coactivator 2 (PNRC2). Also, the cox proportional hazard regression analysis indicates that low expression of miR-23a-3p patients has a remarkable longer OS. Conclusions: Our results reveals that miR-23a-3p may not only serve as a new biomarker for prognosis but also serve as a new therapeutic strategy in the RCC treatment.
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- 2019
- Full Text
- View/download PDF
4. Corrigendum to 'MiR-23a-3p acts as an oncogene and potential prognostic biomarker by targeting PNRC2 in RCC' [Biomed. Pharmacother. 110 (2019) 656–666]
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Jing Quan, Xiang Pan, Yawen Li, Yimin Hu, Lingzhi Tao, Zuwei Li, Liwen Zhao, Jingyao Wang, Hang Li, Yulin Lai, Liang Zhou, Canbin Lin, Yaoting Gui, Jing Ye, Fangting Zhang, and Yongqing Lai
- Subjects
Therapeutics. Pharmacology ,RM1-950 - Published
- 2019
- Full Text
- View/download PDF
5. A Case Report of Giant Adrenal Ganglioneuroma
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Peijie Chen, Canbin Lin, Lu Jin, Yu Ding, Jian Peng, Zebo Chen, Shangqi Yang, Xiangming Mao, and Yongqing Lai
- Subjects
Ganglioneuroma ,Adrenal tumor ,Laparoscopic adrenalectomy ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Adrenal ganglioneuroma (AGN) is an extremely rare and benign entity comprising Schwann cells and ganglion cells. If the lesion approaches to 6 cm, laparoscopic resection is considered as relative contraindication. Now we present a giant adrenal AGN in a 25-year-old male patient. After abdominal computed tomography (CT) scanning, the patient underwent an exploratory laparotomy with right adrenalectomy and histopathological examination further confirmed the lesions as giant AGN, which measured 7 cm × 5 cm × 3.5 cm. Because giant AGN with lesion size greater than 6 cm should be treated by open surgery, it is significant to report the laparoscopic adrenalectomy in patients with large adrenal lesions.
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- 2017
- Full Text
- View/download PDF
6. Laparoscopic radical nephroureterectomy in the oblique supine lithotomy integrative position for rare renal malignant perivascular epithelioid cell tumor with renal vein cancerous thrombosis: A case report and literature review
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Canbin Lin, Shulin Liang, Yongxing Wang, Aidi Liang, Weiting Qin, Jiapeng Huang, Hao Meng, Hong Liu, Ming Chen, and Lei Meng
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Neuroendocrine Tumors ,Perivascular Epithelioid Cell Neoplasms ,Humans ,Female ,Kidney Diseases ,Laparoscopy ,Sarcoma ,Thrombosis ,General Medicine ,Kidney ,Nephroureterectomy ,Renal Veins ,Aged - Abstract
Perivascular epithelioid cell tumor (PEComa) is a mesenchymal tumor that arises from perivascular epithelioid cells and can differentiate into melanocytes and smooth muscle cells. Malignant renal perivascular epithelioid cell tumor is extremely rare. Due to the lack of specific clinical manifestations and imaging features, diagnosing PEComa depends on postoperative pathology and immunohistochemistry. Surgery is the primary treatment for malignant PEComa because the efficacy of radiotherapy and chemotherapy is uncertain. There is still a lack of unified diagnostic criteria and treatment guidelines for renal malignant PEComa, especially with vascular invasion. Hence, the treatment experience depends on a small number of cases reported worldwide.A 68-year-old woman was admitted to our hospital due to intermittent hematuria for over 8 months. The color Doppler ultrasound and computed tomography scan revealed a mass in the lower middle part of the left kidney.Rare renal malignant perivascular epithelioid cell tumor with renal vein cancerous thrombosis.A laparoscopic radical left nephroureterectomy in the oblique supine lithotomy position was performed.The operation process went smoothly, and no pulmonary embolism occurred after the operation. The final pathological diagnosis was a renal malignant perivascular epithelioid cell tumor. After a 12-month follow-up, no recurrence or metastasis was found.Renal malignant PEComa is an extremely rare mesenchymal tumor diagnosed mainly based on pathology. Surgery is currently the effective treatment for malignant PEComa. For the surgical treatment of malignant renal PEComa with vascular invasion, laparoscopic radical nephroureterectomy in the oblique supine lithotomy integrative position has many benefits, as exemplified by our current case.
- Published
- 2022
7. Laparoscopic surgery combined with the double-J tube implantation for the rare cystic-solid schwannoma of seminal vesicle: A Case Report and Literature Review
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Canbin Lin, Aidi Liang, Shulin Liang, Xiao Wang, Lei Meng, and Ming Chen
- Subjects
Male ,Semen ,Genital Neoplasms, Male ,Humans ,Seminal Vesicles ,Laparoscopy ,General Medicine ,Middle Aged ,Neurilemmoma ,Pelvic Neoplasms - Abstract
Schwannoma is common in young and middle-aged people and occurs in the head, neck, posterior mediastinum, and retroperitoneal. Schwannoma, on the other hand, is a rare occurrence in the seminal vesicle. Early diagnosis and treatment are crucial since the disease can cause lower abdominal pain, nocturia, frequent urination, blood sperm, and other symptoms. There is no standard diagnostic or treatment guideline for seminal vesicle schwannomas currently. Therefore, the treatment experience relies on the few cases reported throughout the world.A 45-year-old male patient discovered that the tumor beside the right side spermatophore is bigger than 3 years ago.Schwannoma of seminal vesicle.Ureter double-J tube implantation and laparoscopic surgery for schwannoma of seminal vesicle.The operation process went smoothly. And the patient was no discomfort after half a year.Schwannoma of the seminal vesicle is very rare in the clinic, and the imaging examination was not conclusive. The diagnosis mainly depends on pathological results. Surgical resection is the best treatment method for schwannoma. In surgery for schwannoma of seminal vesicle, combined with the ureter double-J tube implantation are many benefits. This case is an excellent example of the seminal vesicle schwannomas.
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- 2022
8. Oncogenic miR-663a is associated with cellular function and poor prognosis in renal cell carcinoma
- Author
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Zuwei Li, Canbin Lin, Yaoting Gui, Jing Quan, Hang Li, Liang Zhou, Xin Guan, Xiang Pan, Jinling Xu, Peijie Chen, Yongqin Lai, Yulin Lai, and Weijie Xu
- Subjects
Adult ,Male ,0301 basic medicine ,Carcinogenesis ,Biology ,urologic and male genital diseases ,medicine.disease_cause ,Metastasis ,Malignant transformation ,03 medical and health sciences ,0302 clinical medicine ,Cell Movement ,Renal cell carcinoma ,microRNA ,Biomarkers, Tumor ,medicine ,Humans ,Carcinoma, Renal Cell ,Survival analysis ,Aged ,Pharmacology ,Oncogene ,Cell growth ,Oncogenes ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,Kidney Neoplasms ,MicroRNAs ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cancer research ,Female - Abstract
Background MicroRNA(miRNA) plays a key regulatory role in various stages of tumorigenesis, including cell growth, cell cycle control, apoptosis avoidance, tissue invasion, and metastasis. Several microRNAs are involved in the development of renal cell carcinoma (RCC) and the malignant transformation process. However, the effects of miR-663a on RCC have rarely been reported. Methods In the present study, the expression of miR-663a was examined in RCC using matched normal kidney tissues and four cell lines (786-O, Caki-1, ACHN and HK-2). MicroRNA mimics were transiently transfected into RCC cells and the effects of over expression on proliferation, migration, invasion, and apoptosis was observed. In addition, the relationship between miR-663a expression in 42 formalin-fixed paraffin-embedded (FFPE) clear cell renal carcinoma (ccRCC) samples and clinical pathological variables and overall survival was investigated. We evaluated the prognostic value of miR-663a expression in ccRCC by experimental results. Results The results showed that the expression of miR-663a was up-regulated in RCC cells and tissues and miR-663a was associated with proliferation, migration, invasion, and apoptosis of RCC. Cox proportional hazard regression analysis showed that a high expression of miR-663a patients had a significantly shorter overall survival in univariate and multivariate analysis. Kaplan-Meier survival curves showed that a high expression of miR-663a patients had a significantly shorter overall survival. Conclusions These results indicate that miR-663a can be used as an independent marker for the poor prognosis of ccRCC, and may also play an important role as a tumor oncogene in the occurrence and development of RCC.
- Published
- 2018
9. Oncogene miR-187-5p is associated with cellular proliferation, migration, invasion, apoptosis and an increased risk of recurrence in bladder cancer
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Liwen Zhao, Liang Zhou, Canbin Lin, Yongqing Lai, Zuwei Li, Xiqi Peng, Weiqing Li, Yun Chen, Jinling Xu, Xiang Pan, Xin Guan, Weijie Xu, Jing Quan, and Hang Li
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Male ,0301 basic medicine ,Cell ,Apoptosis ,Transfection ,Malignancy ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,Cell Movement ,Risk Factors ,Cell Line, Tumor ,microRNA ,medicine ,Humans ,Aged ,Cell Proliferation ,Proportional Hazards Models ,Aged, 80 and over ,Pharmacology ,Wound Healing ,Bladder cancer ,Oncogene ,medicine.diagnostic_test ,Cell growth ,business.industry ,Reproducibility of Results ,General Medicine ,Middle Aged ,medicine.disease ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Urinary Bladder Neoplasms ,030220 oncology & carcinogenesis ,Cancer research ,Female ,Neoplasm Recurrence, Local ,business - Abstract
Background Bladder cancer, the ninth-most-common malignancy worldwide with an estimated 356,000 new cases and 145,000 deaths annually, has a propensity to relapse, requiring lifelong monitoring after diagnosis. 75% patients diagnosed with BC are non-muscle invasive BC and over 50% of them experience recurrences within 6–12 years of initial diagnosis. miRNA are small, noncoding RNA and shown to be oncogenes or anti-oncogenes in bladder cancer, contributing to numerous BC cell processes, including cell proliferation, differentiation, migration and apoptosis. Methods RT-qPCR were performed to detect the expression of miR-187-5p in tissues and cell lines, After which, clinicopathological variables and the prognostic value of altered miR-187-5p expression in BC was analyzed with the 48 formalin-fixed paraffin-embedded BC samples. Moreover, Cell functional assays (wound healing assay, CCK-8 assay, transwell assay and flow cytometry assay) were performed to explore the relationship between miR-187-5p expression and cell proliferation, migration, invasion and apoptosis in BC. Results Up-regulation of miR-187-5p was observed in BC tissues and BC cell lines. Cox proportional hazard regression analysis demonstrated that the patients with low expression of miR-187-5p experience lower risks of recurrence in the univariate and multivariate analysis. The Kaplan-Meier recurrence-free curves suggested that the patients with low expression of miR-187-5p experience lower risks of recurrence. Up-regulation of miR-187-5p promotes cell proliferation and mobility and inhibits the apoptosis of 5637 and UM-UC-3 cell, while down-regulation of miR-187-5p reverses these effects. Conclusions The results of our study demonstrated that oncogene miR-187-5p is associated with cellular proliferation, migration, invasion, apoptosis and an increased risk of recurrence in bladder cancer.
- Published
- 2018
10. Circular RNA atlas in osteoclast differentiation with and without alendronate treatment
- Author
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Weibin Lin, Shaofeng Ma, Canbin Lin, Changqing Chen, Zhenqi Ding, Guofeng Huang, Cong Zhu, and Jianbiao Lin
- Subjects
0301 basic medicine ,lcsh:Diseases of the musculoskeletal system ,MCSF ,THP-1 Cells ,Gene Expression ,Osteoclasts ,Andrology ,03 medical and health sciences ,0302 clinical medicine ,Western blot ,lcsh:Orthopedic surgery ,Osteoclast ,medicine ,Cathepsin K ,Humans ,Orthopedics and Sports Medicine ,Receptor ,Messenger RNA ,biology ,medicine.diagnostic_test ,Alendronate ,Bone Density Conservation Agents ,business.industry ,Macrophage Colony-Stimulating Factor ,RANK Ligand ,Acid phosphatase ,RANKL ,Cell Differentiation ,RNA, Circular ,Blot ,lcsh:RD701-811 ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Depression, Chemical ,biology.protein ,Osteoporosis ,Surgery ,lcsh:RC925-935 ,business ,Research Article - Abstract
Background Alendronate (AL) is the most widely used bisphosphonate in the treatment of osteoporosis (OP). However, the role of circular RNAs (circRNAs) in the treatment of OP with AL remains unclear. Methods In this study, we showed that osteoclast (OC) precursors (OPCSs) could be induced into OCs with macrophage colony-stimulating factor (MCSF) and receptor activator of nuclear factor-κB ligand (RANKL) treatment. Subsequently, the OCs were treated with AL. OC differentiation-related biomarkers including RANK, tartrate-resistant acid phosphatase (TRAP), and cathepsin K (CTSK) were analyzed with TRAP staining, quantitative real-time (qPCR), and western blotting. Differentially expressed circRNAs (DECs) were identified among the OPCS, OC, and OC + AL groups. In addition, the expression levels of 10 DECs related to OC differentiation were verified by qPCR. Results TRAP staining showed that MCSF and RANKL treatment effectively induced OPCSs to differentiate into OCs. In addition, qPCR and western blot analysis revealed that the three biomarkers of OC (RANK, TRAP, and CTSK) were expressed significantly more in the OC group than those in the OPCS group. In contrast, the mRNA and protein expression levels of these three biomarkers decreased significantly in OCs treated with AL compared with those non-treated OCs. GO analysis of the DECs in the OPCS group vs. the OC group revealed that their functions were mainly related to cell, cell part, binding, and single-organism terms. KEGG analysis of the top 20 DECs in a comparison between the OPCS and OC groups showed that genes involved in mitogen-activated protein kinase signaling were the most common. Results of functional analyses of DECs in an OC vs. OC + AL comparison were similar to those in the OPCS vs. OC comparison. Finally, qPCR showed that, in the OC + AL vs. OC group comparison, the expression levels of seven and three DECs significantly decreased and increased, respectively. Conclusions Having successfully induced OPCSs to differentiate into OCs, we showed that AL suppresses the differentiation of OPCS into OC and that 10 DECs were involved in the regulation of this process. This indicates that these DECs might be important to the treatment of OP.
- Published
- 2020
11. Oncogenic miR-425-5p is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma
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Yawen Li, Fangting Zhang, Xionghui Wu, Canbin Lin, Zebo Chen, Xiang Pan, Lingzhi Tao, Liwen Zhao, Jing Quan, Liang Zhou, Yu Ding, Yulin Lai, Yongqing Lai, Jing Ye, Tao He, Liangchao Ni, Shuolei Sun, and Yimin Hu
- Subjects
0301 basic medicine ,Cancer Research ,renal cell carcinoma ,Oncogene ,microRNA ,Chemistry ,Cell ,Cell migration ,Articles ,Cell cycle ,microRNA-425-5p ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Oncology ,Downregulation and upregulation ,Cell culture ,Apoptosis ,oncogene ,030220 oncology & carcinogenesis ,medicine ,Cancer research ,Viability assay - Abstract
An increasing number of studies have demonstrated the function of microRNAs (miRNAs) in the initiation and development of various types of cancer. Among them, miR-425-5p is proven to serve an important function in several types of cancer, including gastric, cervical cancer, and hepatocellular carcinoma. However, the function of miR-425-5p in renal cell carcinoma (RCC) remains unclear. In the present study, it was demonstrated that the expression level of miR-425-5p was upregulated in RCC tissues and cell lines compared with normal tissues and cell lines (P
- Published
- 2018
12. Oncogenic miR-23a-5p is associated with cellular function in RCC
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Tao He, Hui Zeng, Peijie Chen, Yongqing Lai, Xiang Pan, Jing Quan, Shangqi Yang, Canbin Lin, Yulin Lai, and Lu Jin
- Subjects
Adult ,Male ,0301 basic medicine ,Cancer Research ,Cell Survival ,Cell ,Down-Regulation ,Biology ,urologic and male genital diseases ,Biochemistry ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Cell Movement ,Cell Line, Tumor ,microRNA ,Genetics ,medicine ,Humans ,Carcinoma, Renal Cell ,Molecular Biology ,Aged ,Cell Proliferation ,medicine.diagnostic_test ,Oncogene ,Cell growth ,Antagomirs ,Middle Aged ,Cell cycle ,Molecular medicine ,Kidney Neoplasms ,Up-Regulation ,MicroRNAs ,HEK293 Cells ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,Molecular Medicine ,Female - Abstract
In recent years, accumulating evidence has demonstrated that microRNAs (miRs, miRNAs) may serve an important role in the occurrence and development of tumors. miR‑23a‑5p has been confirmed as an oncogene in numerous diseases through gene chip analysis. However, as the most common type of renal tumor, the expression and function of miR‑23a‑5p in renal cell carcinoma (RCC) remains unclear. In the present study, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis, and Cell Counting Kit‑8 (CCK‑8), wound scratch, Transwell, MTT and flow cytometry assays were performed to investigate the role of miR‑23a‑5p in RCC. The expression of miR‑23a‑5p in RCC tissue samples was significantly higher compared with that in normal tissue samples (P
- Published
- 2017
13. miR-625-3p promotes migration and invasion and reduces apoptosis of clear cell renal cell carcinoma
- Author
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Liwen, Zhao, Kaihao, Liu, Xiang, Pan, Jing, Quan, Liang, Zhou, Zuwei, Li, Canbin, Lin, Jinling, Xu, Weijie, Xu, Xin, Guan, Hang, Li, Liangchao, Ni, Yaoting, Gui, and Yongqing, Lai
- Subjects
Original Article - Abstract
Clear cell renal cell carcinoma (ccRCC) is a common malignancy, yet, the mechanisms underlying tumorigenesis remain unclear. Several miRNAs have been implicated in the development of RCC previously via regulation of target gene expression. As miR-625-3p has recently been identified to play a role in development of other malignancies and is reportedly upregulated in ccRCC, we sought to investigate the role of this miRNA in the progression of ccRCC. Analysis of 30 paired fresh ccRCC tissues and adjacent normal renal tissues revealed that the expression of miR-625-3p was increased in ccRCC tissues compared to normal tissues. Subsequently, in 136 formalin-fixed paraffin-embedded ccRCC tissues, the increased miR-625-3p expression was correlated with poor prognosis for ccRCC patients. The diagnostic value of miR-625-3p was identified in 50 ccRCC patients and 74 healthy controls by ROC curve. miR-625-3p was decreased in serum of ccRCC patients compared to healthy individuals. miR-625-3p could serve as a promising serum biomarker for yielding an area under the receiver operating characteristic curve of 0.792 with 70.3% sensitivity and 80.0% specificity in discriminating ccRCC from healthy individuals. Using in vitro functional assays, we found that overexpression of miR-625-3p promoted migration and invasion of ccRCC cells but reduced ccRCC cell apoptosis. Inhibition of miR-625-3p, on the other hand, exerted the opposite effects. Bioinformatic analyses indicated that predicted gene targets of miR-625-3p are correlated with lower overall survival of ccRCC patients. Together, these findings demonstrate that miR-625-3p promotes ccRCC migration and invasion and reduces apoptosis, providing a prognostic marker for survival and a potential diagnostic and therapeutic target against ccRCC.
- Published
- 2019
14. MiR-23a-3p acts as an oncogene and potential prognostic biomarker by targeting PNRC2 in RCC
- Author
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Jingyao Wang, Jing Quan, Yawen Li, Hang Li, Zuwei Li, Jing Ye, Canbin Lin, Yimin Hu, Fangting Zhang, Yaoting Gui, Liwen Zhao, Liang Zhou, Yulin Lai, Lingzhi Tao, Xiang Pan, and Yongqing Lai
- Subjects
0301 basic medicine ,PNRC2 ,Adult ,Male ,Receptors, Cytoplasmic and Nuclear ,RM1-950 ,Biology ,medicine.disease_cause ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,Cell Line, Tumor ,microRNA ,medicine ,Biomarkers, Tumor ,Gene silencing ,Humans ,Viability assay ,Carcinoma, Renal Cell ,Oncogene ,Aged ,Pharmacology ,medicine.diagnostic_test ,Cell growth ,General Medicine ,Oncogenes ,Middle Aged ,Prognosis ,Renal cell carcinoma ,Kidney Neoplasms ,Survival Rate ,MicroRNAs ,030104 developmental biology ,miR-23a-3p ,HEK293 Cells ,030220 oncology & carcinogenesis ,Cancer research ,Trans-Activators ,Biomarker (medicine) ,Female ,Therapeutics. Pharmacology ,Carcinogenesis ,Biomarkers - Abstract
Background Renal cell carcinoma (RCC) is a most common kidney malignancy, with atypical symptoms in the early stage and poor outcome in the late stage. Recently, emerging evidence revealed that some miRNAs play an essential role in the tumorigenesis and progression of RCC. Therefore, the aim of this study is that understand the detailed molecular mechanism of miR-23a-3p in RCC and identify its potential clinical value. Methods In this study, RT-qPCR, wound scratch assay, cell proliferation assay, transwell assay and flow cytometry assay were performed to detect miR-23a-3p expression and its proliferation, migration and apoptosis in RCC. The bioinformatics analysis, RT-qPCR, western blot and luciferase reporter assay were performed to discern and examine the relationship between miR-23a-3p and its potential targets. Moreover, we analyzed the relationship between miR-23a-3p expression and clinicopathological variables or overall survival (OS) from 118 formalin-fixed paraffin-embedded RCC samples. Results miR-23a-3p is significantly up-regulated in RCC tissue samples, RCC cell lines and the TCGA database. Upregulating miR-23a-3p enhances, while silencing miR-23a-3p suppresses cell viability, proliferation and mobility in ACHN and 786-O cell lines. Besides, overexpression of miR-23a-3p inhibits the cell apoptosis. Then our study further reveals that miR-23a-3p regulates tumorigenesis by targeting Proline-Rich Nuclear Receptor Coactivator 2 (PNRC2). Also, the cox proportional hazard regression analysis indicates that low expression of miR-23a-3p patients has a remarkable longer OS. Conclusions Our results reveals that miR-23a-3p may not only serve as a new biomarker for prognosis but also serve as a new therapeutic strategy in the RCC treatment.
- Published
- 2018
15. Primary small‑cell neuroendocrine carcinoma of the urinary bladder: A rare case and a review of the literature
- Author
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Peijie Chen, Yongqing Lai, Canbin Lin, Zuwei Li, Shangqi Yang, Shuolei Sun, Yu Yang, Cankun Zhou, Danyun Wang, Jiansen Xie, and Jian Peng
- Subjects
Cancer Research ,Pathology ,medicine.medical_specialty ,Bladder cancer ,Urinary bladder ,biology ,business.industry ,Cancer ,Articles ,urologic and male genital diseases ,medicine.disease ,Molecular medicine ,medicine.anatomical_structure ,Oncology ,Rare case ,medicine ,Synaptophysin ,biology.protein ,Immunohistochemistry ,business ,Pathological - Abstract
Primary small-cell neuroendocrine carcinoma (SCNEC) of the urinary bladder is a rare tumor characterized by poor differentiation and high aggressiveness. Only ~150 cases have been reported in the literature to date. We herein present a case of an 87-year-old man who presented with hematuria and was found to have an ill-defined mass in the urinary bladder on computed tomography and cystoscopic examination. On pathological examination following tumor biopsy, the mucosa of the bladder wall was found to be extensively infiltrated by neuroendocrine carcinoma, positive for CD56 and synaptophysin and negative for epithelial membrane antigen, consistent with SCNEC of the urinary bladder. The patient refused further surgical treatment and succumbed to the disease 2 months after the diagnosis. In the present study, this rare case of primary SCNEC of the urinary bladder is presented, along with a discussion on the clinical presentation, immunohistochemical and cytomorphological characteristics, management, biological behavior and prognosis of this disease.
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- 2018
16. MiR-302b regulates cell functions and acts as a potential biomarker to predict recurrence in bladder cancer
- Author
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Yongqing Lai, Canbin Lin, Zuwei Li, Xin Guan, Jiansen Xie, Yun Chen, Weijie Xu, Jing Quan, Hang Li, Liwen Zhao, Liang Zhou, Jinling Xu, and Xiang Pan
- Subjects
0301 basic medicine ,Male ,Cell ,Apoptosis ,General Biochemistry, Genetics and Molecular Biology ,Metastasis ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,Cell Movement ,microRNA ,medicine ,Biomarkers, Tumor ,Humans ,General Pharmacology, Toxicology and Pharmaceutics ,Cell Proliferation ,Bladder cancer ,medicine.diagnostic_test ,business.industry ,Cancer ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Urinary Bladder Neoplasms ,030220 oncology & carcinogenesis ,Cancer research ,Biomarker (medicine) ,Female ,Neoplasm Recurrence, Local ,business - Abstract
Background Bladder cancer is the most common urogenital tumor with substantial morbidity, high recurrence rate and mortality. miRNAs, a class of endogenous noncoding RNA, were found to involve in the genesis, maintenance and metastasis of cancer. Genomic profiling revealed that miR-302b is down-regulated in bladder cancer while its functions in bladder cancer remain to be ascertained. Methods Cell functional assays including wound healing assay, CCK-8 assay, Transwell assay and flow cytometry assay were performed to clarify the functions of miR-302b expression in cell proliferation, migration, invasion and apoptosis in BC. Furthermore, RT-qPCR was performed to study the expression of miR-302b in bladder cancer tissues and the prognostic value of altered miR-302b expression with 48 formalin-fixed paraffin-embedded bladder urothelial carcinoma samples. Results The results of RT-qPCR demonstrated that expression level of miR-302b was significantly reduced in bladder cancer tissues and cell lines. The cells after transfected with miR-302b mimic showed lower mobility, lower proliferation and increased apoptosis, while opposite results were obtained after inhibiting the expression of miR-302b. The prognosis analysis demonstrated that the patients with low expression of miR-302b experienced high risks of recurrence. Conclusions The results of our study demonstrate that miR-302b regulates cell functions and acts as a potential biomarker to predict recurrence in bladder cancer.
- Published
- 2018
17. Oncogene miR-154-5p regulates cellular function and acts as a molecular marker with poor prognosis in renal cell carcinoma
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Jing Quan, Weijie Xu, Hang Li, Yimin Hu, Canbin Lin, Liwen Zhao, Liang Zhou, Zuwei Li, Tao He, Yulin Lai, Yongqing Lai, Xin Guan, Jinling Xu, Xiang Pan, Shangqi Yang, and Peijie Chen
- Subjects
0301 basic medicine ,Adult ,Male ,Apoptosis ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Downregulation and upregulation ,Renal cell carcinoma ,Cell Movement ,medicine ,Tumor Cells, Cultured ,Humans ,MTT assay ,General Pharmacology, Toxicology and Pharmaceutics ,Carcinoma, Renal Cell ,Aged ,Cell Proliferation ,Oncogene ,Cell growth ,business.industry ,Cancer ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,Carcinoma, Papillary ,Kidney Neoplasms ,Gene Expression Regulation, Neoplastic ,Survival Rate ,MicroRNAs ,030104 developmental biology ,Real-time polymerase chain reaction ,030220 oncology & carcinogenesis ,Cancer research ,Female ,business - Abstract
Aims In adult population, the renal cell carcinoma (RCC) is one of the most common urological malignancies. It is meaningful to research for the molecular markers which are involved in the occurrence and development of RCC. Therefore, we concentrate on illuminating the role of microRNA-154-5p in progression of RCC and explore its prognostic values. Main methods The real-time quantitative polymerase chain reaction (RT-qPCR) was applied to determine expression level of miR-154-5p in tissues. Afterwards, the transfected cell lines ACHN and 786-O were used for the CCK-8 assay, MTT assay, wound healing assay, transwell assay and flow cytometric assay to explore the role of miR-154-5p in regulating cellular function. In addition, formalin-fixed paraffin-embedded (FFPE) renal cancer samples were used for detecting the relationship between expression level of miR-154-5p and clinical information. Furthermore, univariate and multivariate Cox proportional-hazards regression analyses, and the Kaplan-Meier survival curves were performed to evaluate the prognostic value of miR-154-5p in RCC. Key findings The RT-qPCR indicated that miR-154-5p is up-regulated in RCC pathologic specimens and cell lines. Results of study also demonstrated that upregulation of miR-154-5p reduced cell apoptosis and promoted cell proliferation, viability, migration as well as invasion in RCC cells. The prognosis analyses indicated that the expression level of miR-154-5p is associated with the prognosis of renal cancer, and the overall survival of patients with low expression is longer. Significance The present study revealed that the oncogene miR-154-5p regulates cellular function and acts as a molecular marker with poor prognosis in renal cell carcinoma.
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- 2018
18. miR-216a-5p acts as an oncogene in renal cell carcinoma
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Shangqi Yang, Jing Quan, Weijie Xu, Peijie Chen, Yongqing Lai, Jinling Xu, Lu Jin, Zebo Chen, Xin Guan, Yun Chen, Canbin Lin, and Liangchao Ni
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0301 basic medicine ,Cancer Research ,Oncogene ,Cell ,Motility ,Cancer ,Articles ,General Medicine ,Cell cycle ,Biology ,urologic and male genital diseases ,medicine.disease ,Molecular medicine ,Metastasis ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Immunology and Microbiology (miscellaneous) ,030220 oncology & carcinogenesis ,microRNA ,medicine ,Cancer research - Abstract
MiR-216a-5p has been acknowledged as an oncogene and is known to be involved in the progression and metastasis of numerous cancer subtypes. However, the potential role of miR-216a-5p in renal cell carcinoma (RCC) remains to be elucidated. In the present study, reverse transcription-quantitative polymerase chain reaction was performed to detect the expression levels of miR-216a-5p in RCC tissues. Cell counting kit-8, MTT, wound scratch, Transwell and flow cytometric assays were performed to establish the biological functions of miR-216a-5p in RCC. Functional experiments demonstrated that the expression of miR-216a-5p was upregulated in RCC (P
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- 2018
19. Tumor suppressor microRNA‑136‑5p regulates the cellular function of renal cell carcinoma
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Jinling Xu, Liwen Zhao, Xueling Wu, Canbin Lin, Lu Jin, Yongqing Lai, Xin Guan, Weijie Xu, Yong Wang, Peijie Chen, Tao Wang, Xiang Pan, and Shangqi Yang
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0301 basic medicine ,Cancer Research ,Oncogene ,medicine.diagnostic_test ,Cell growth ,Cell ,Articles ,Cell cycle ,Biology ,urologic and male genital diseases ,Molecular medicine ,female genital diseases and pregnancy complications ,Flow cytometry ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,microRNA ,medicine ,Cancer research ,Signal transduction - Abstract
MicroRNAs (miRs) are involved in diverse physiological and developmental processes at the post-transcriptional level in cells. Previous studies have demonstrated that miR-136-5p is involved in certain types of cancer. However, the function of miR-136-5p in renal cell carcinoma (RCC) remains to be fully elucidated. In present study, miR-136-5p expression levels were determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and MTT assays, CCK-8 assays, Transwell assays, wound healing assays and flow cytometry were performed to investigate the function of miR-136-5p in RCC. RT-qPCR revealed that the expression of miR-136 was significantly lower in RCC tissues and cells compared with adjacent non-tumor tissues and cells in vitro. miR-136-5pwas also demonstrated to be associated with RCC cell proliferation, viability, migration, invasion and apoptosis. miR-136-5p may therefore function as a tumor suppressor in RCC. Further studies are required to elucidate the molecular mechanisms and signaling pathways underlying these functions of miR-136-5p, to investigate the potential function of miR-136-5p as a biomarker for the early detection and prognosis of RCC, and its potential as a therapeutic target for the treatment of RCC.
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- 2018
20. MicroRNA‑191‑5p exerts a tumor suppressive role in renal cell carcinoma
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Tao He, Xueling Wu, Peijie Chen, Lu Jin, Jing Quan, Xiang Pan, Liwen Zhao, Shangqi Yang, Yongqing Lai, Yong Wang, Liangchao Ni, Liang Zhou, and Canbin Lin
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0301 basic medicine ,Cancer Research ,Oncogene ,medicine.diagnostic_test ,Cell growth ,Cell ,Articles ,General Medicine ,Biology ,Cell cycle ,Flow cytometry ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Immunology and Microbiology (miscellaneous) ,Cell culture ,Apoptosis ,030220 oncology & carcinogenesis ,microRNA ,medicine ,Cancer research - Abstract
Renal cell carcinoma (RCC) is a common tumor of the urinary system. Previously, miR-191-5p has been reported to be associated with various types of cancer; however, its specific functions in RCC have not been investigated to date. In the present study, the expression of miR-191-5p in the 786-O and ACHN cell lines was detected in vitro by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The results of RT-qPCR revealed that miR-191-5p was significantly downregulated in the two cell lines compared with the 293T cell line. miR-191-5p was also significantly downregulated in RCC tissue compared with paired normal tissue. In addition, the effects of miR-191-5p on cell proliferation, migration, invasion and apoptosis were examined by CCK-8, MTT, wound scratch, Transwell and flow cytometry assays. Downregulation of miR-191-5p was observed to promote cell proliferation, migration and invasion, as well as to repress the cell apoptosis of 786-O and ACHN cells. Therefore, the current study suggests that miR-191-5p functions as a tumor suppressor in RCC. Further studies are required to uncover the underlying signaling pathway of miR-191-5p and its potential role as a biomarker for early detection and prognosis prediction, and as a therapeutic target of RCC.
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- 2017
21. Tuberous sclerosis-associated renal angiomyolipoma: A report of two cases and review of the literature
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Xionghui Wu, Canbin Lin, Yu Yang, Shangqi Yang, Yu Ding, Liangchao Ni, Lu Jin, and Yongqing Lai
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Cancer Research ,Pathology ,medicine.medical_specialty ,Abdominal pain ,education.field_of_study ,Flank pain ,business.industry ,Population ,030232 urology & nephrology ,Cancer ,Disease ,Articles ,medicine.disease ,03 medical and health sciences ,Tuberous sclerosis ,0302 clinical medicine ,Oncology ,medicine ,Hamartoma ,Radiology ,medicine.symptom ,education ,business ,030217 neurology & neurosurgery ,Renal angiomyolipoma - Abstract
In the general population, tuberous sclerosis-associated renal angiomyolipoma (RAML) is a rare benign hamartoma with potentially life-threatening complications and a poor prognosis. The two patients reported in the present study, who were diagnosed with bilateral RAML and spontaneous rupture of the RAML with tuberous sclerosis, presented with flank pain, abdominal pain, hemorrhage, hematuria and multiple lesions. The two cases are representative examples of the disease, and highlight the importance of determining the risk of acute hemorrhage in the early stages, and the significance of timely and proper treatment.
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- 2017
22. Oncogenic miR-100-5p is associated with cellular viability, migration and apoptosis in renal cell carcinoma
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Tao He, Yong Wang, Jing Quan, Yongqing Lai, Liangchao Ni, Tao Wang, Shangqi Yang, Xueling Wu, Xiang Pan, Peijie Chen, Canbin Lin, Yulin Lai, and Liang Zhou
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0301 basic medicine ,Adult ,Male ,Cancer Research ,Cell Survival ,Cell ,Apoptosis ,Biology ,Biochemistry ,Flow cytometry ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Cell Movement ,Cell Line, Tumor ,Genetics ,medicine ,Humans ,Viability assay ,Molecular Biology ,Carcinoma, Renal Cell ,Cells, Cultured ,Cell Proliferation ,Neoplasm Staging ,Oncogene ,medicine.diagnostic_test ,Cell growth ,Oncogenes ,Cell cycle ,Middle Aged ,Kidney Neoplasms ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Cell culture ,030220 oncology & carcinogenesis ,Cancer research ,Molecular Medicine ,Female ,Neoplasm Grading ,A431 cells - Abstract
As influencing factors of genesis and progression in several types of human tumor, microRNAs (miRs) serves roles in the regulation of tumor cell viability, migration, and apoptosis. The present research aimed to investigate the association between the function of miR‑100‑5p and renal cell carcinoma (RCC). miR‑100‑5p expression was determined in RCC tissue and paired normal tissue samples using reverse transcription‑quantitative polymerase chain reaction. To assess the effects of miR‑100‑5p on cell viability, migration and apoptosis, multiple methods were used, including scratch wound assays, MTT assays, and flow cytometry. It was demonstrated that miR‑100‑5p was significantly upregulated in RCC tissue compared with in normal adjacent tissue samples. Furthermore, the viability and migration of 786‑O and, ACHN cells tranfected with miR‑100‑5p was significantly increased compared with the negative control group. In addition, miR‑100‑5p‑transfected 786‑O and ACHN cells demonstrated significantly reduced cellular apoptotic rates compared with the negative control group. To the best of our knowledge, the present study is the first to report an association between miR‑100‑5p and RCC. The results of the current study suggest that tumor oncogene miR‑100‑5p could be used as a diagnostic biomarker for RCC.
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- 2017
23. Corrigendum to 'MiR-23a-3p acts as an oncogene and potential prognostic biomarker by targeting PNRC2 in RCC' [Biomed. Pharmacother. 110 (2019) 656–666]
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Yimin Hu, Hang Li, Lingzhi Tao, Yongqing Lai, Liang Zhou, Canbin Lin, Xiang Pan, Jing Quan, Liwen Zhao, Jing Ye, Jingyao Wang, Yulin Lai, Fangting Zhang, Yawen Li, Yaoting Gui, and Zuwei Li
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Pharmacology ,Oncogene ,business.industry ,Cancer research ,Medicine ,Prognostic biomarker ,Therapeutics. Pharmacology ,RM1-950 ,General Medicine ,business - Published
- 2019
24. Oncogenic miR‑100‑5p is associated with cellular viability, migration and apoptosis in renal cell carcinoma.
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PEIJIE CHEN, CANBIN LIN, JING QUAN, YULIN LAI, TAO HE, LIANG ZHOU, XIANG PAN, XUELING WU, LIANGCHAO NI, SHANGQI YANG, TAO WANG, YONGQING LAI, and YONG WANG
- Subjects
- *
RENAL cell carcinoma , *TUMORS , *CELL proliferation , *APOPTOSIS , *CANCER invasiveness - Abstract
As influencing factors of genesis and progression in several types of human tumor, microRNAs (miRs) serves roles in the regulation of tumor cell viability, migration, and apoptosis. The present research aimed to investigate the association between the function of miR‑100‑5p and renal cell carcinoma (RCC). miR‑100‑5p expression was determined in RCC tissue and paired normal tissue samples using reverse transcription‑quantitative polymerase chain reaction. To assess the effects of miR‑100‑5p on cell viability, migration and apoptosis, multiple methods were used, including scratch wound assays, MTT assays, and flow cytometry. It was demonstrated that miR‑100‑5p was significantly upregulated in RCC tissue compared with in normal adjacent tissue samples. Furthermore, the viability and migration of 786‑O and, ACHN cells tranfected with miR‑100‑5p was significantly increased compared with the negative control group. In addition, miR‑100‑5p‑transfected 786‑O and ACHN cells demonstrated significantly reduced cellular apoptotic rates compared with the negative control group. To the best of our knowledge, the present study is the first to report an association between miR‑100‑5p and RCC. The results of the current study suggest that tumor oncogene miR‑100‑5p could be used as a diagnostic biomarker for RCC. [ABSTRACT FROM AUTHOR]
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- 2017
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- View/download PDF
25. Oncogenic miR-23a-5p is associated with cellular function in RCC.
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JING QUAN, LU JIN, XIANG PAN, TAO HE, YULIN LAI, PEIJIE CHEN, CANBIN LIN, SHANGQI YANG, HUI ZENG, and YONGQING LAI
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ONCOGENIC viruses ,MICRORNA ,TUMORS ,ONCOGENES ,GENETIC transcription - Abstract
In recent years, accumulating evidence has demonstrated that microRNAs (miRs, miRNAs) may serve an important role in the occurrence and development of tumors. miR-23a-5p has been confirmed as an oncogene in numerous diseases through gene chip analysis. However, as the most common type of renal tumor, the expression and function of miR-23a-5p in renal cell carcinoma (RCC) remains unclear. In the present study, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis, and Cell Counting Kit-8 (CCK-8), wound scratch, Transwell, MTT and flow cytometry assays were performed to investigate the role of miR-23a-5p in RCC. The expression of miR-23a-5p in RCC tissue samples was significantly higher compared with that in normal tissue samples (P<0.01). Furthermore, the expression of miR-23a-5p in RCC cell lines (786O, ACHN and Caki-1) was significantly higher compared with that in the human embryo kidney 293T cell line, as determined using RT-qPCR (P<0.001). In addition, the results revealed that the upregulation of miR-23a-5p promoted the proliferation, migration and invasion of RCC cells, and inhibited RCC cell apoptosis. The downregulation of miR-23a-5p resulted in the reversal of the results described above. Additionally, it was observed that the downregulation of miR-23a-5p significantly promoted ACHN and 786O cell viability (P<0.001). The results of the present study suggest that miR-23a-5p is an oncogene in the occurrence and development of RCC and may be a novel therapeutic target for RCC. [ABSTRACT FROM AUTHOR]
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- 2017
- Full Text
- View/download PDF
26. Tuberous sclerosis-associated renal angiomyolipoma: A report of two cases and review of the literature.
- Author
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CANBIN LIN, LU JIN, YU YANG, YU DING, XIONGHUI WU, LIANGCHAO NI, SHANGQI YANG, and YONGQING LAI
- Subjects
- *
RENAL cancer treatment , *TUBEROUS sclerosis , *HEMORRHAGE - Abstract
In the general population, tuberous sclerosis-associated renal angiomyolipoma (RAML) is a rare benign hamartoma with potentially life-threatening complications and a poor prognosis. The two patients reported in the present study, who were diagnosed with bilateral RAML and spontaneous rupture of the RAML with tuberous sclerosis, presented with flank pain, abdominal pain, hemorrhage, hematuria and multiple lesions. The two cases are representative examples of the disease, and highlight the importance of determining the risk of acute hemorrhage in the early stages, and the significance of timely and proper treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
27. Directional couplers based on long-range air-hole assisted subwavelength waveguides.
- Author
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Weikang Zhou, Xiao Liang, Canbin Lin, Lintao Liu, Jin Tao, and Xu Guang Huang
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DIRECTIONAL couplers ,FINITE difference time domain method ,PHOTONICS ,FINITE difference method ,WAVEGUIDES - Abstract
In this paper, we propose X-type and K-type couplers based on the novel long-range air-hole assisted subwavelength waveguides. A finite-difference time-domain (FDTD) method is used to simulate and analyze their characteristics. The results reveal that both the X-type and K-type couplers have very small transverse widths, which is suitable for high-density photonic integration. The excess losses of two couplers are less than 0.25 dB in one coupling period. Also, they can be integrated with other long-range air-hole assisted subwavelength waveguide devices to constitute large-scale planar integrated functional systems, which cannot be achieved by other waveguide couplers. [ABSTRACT FROM AUTHOR]
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- 2017
- Full Text
- View/download PDF
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