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In this work, we study the neuroprotective properties of lithium ascorbate in in vivo and in vitro stress models. Neurocytological and behavioral studies were carried out on models of stress in both a culture of nerve cells and experimental animals. The studied drug showed a significant neuroprotective effect of lithium ascorbate in neuronal cultures exposed to glutamate toxicity and an adaptogenic effect in stress models in rats. The results suggest that lithium ascorbate possess a high neuroprotective potential in stress models in vivo and in vitro.

Health and productivity of ruminants directly depend on the state of rumen and digestion in intestinal tract. Normal microflora protects the body from pathogenic microbes, stimulates the immune system, participates in metabolic reactions and plays an important role in energy metabolism. Disturbance of species composition of microflora in rumen under effect of various factors leads to a state of dysbiosis, disorders of products digestibility, changes in enzymatic processes and other disorders. Biologically active substances of an adaptogenic nature can show immunomodulatory and antimicrobial activity. Adaptogen lithium ascorbate has stress-protective, neurotrophic, neuroprotective, antioxidant, immunomodulatory properties. In this paper, using molecular genetic methods, the effect of lithium ascorbate on composition of rumen microbiota of sheep was studied for the first time. Composition of microbiota was determined by NGS sequencing method. In total, 37 phylums, 76 classes, 98 orders, 225 families and 894 species of microorganisms were identified. It has been determined that introduction of lithium ascorbate at a dose of 10 mg/kg of body weight into the main diet of sheep contributed to increase in proportion of cellulolytic bacteria from 73.6±1.6% to 75.4±0.9% and over 40% decrease in total number of infusoria in rumen content. Cellulolytic activity of rumen fluid in sheep of the experimental group increased by 38% compared to the animals of the control group. The number of pathogenic bacteria decreased from 2.7% in sheep of the control group to 1.6% in animals of the experimental group, while the content of beneficial bacilli increased from 0.3% to 0.5%, respectively. Content of undesirable and conditionally pathogenic microflora decreased in the sheep of experimental group. The obtained data confirm positive effect of adaptogen lithium ascorbate, introduced into diet at a dosage of 10 mg/kg of body weight, on qualitative and quantitative composition of microbiota in sheep rumen. Therefore, lithium ascorbate, a broad-spectrum adaptogen, can be recommended as a feed additive to the main diet of sheep to improve rumen digestion and increase productivity.

The paper is the fundamental beginning of research series aimed at understanding the processes associated with high performance in higher animals. The research aim is to study correlation of dicarboxylic acid cycle with tricarboxylic acid cycle with establishment of activity and dislocation of enzymes, confirming the hypothesis of availability and active metabolic participation of peroxisome in highly productive animals. Research was conducted on the basis of the VNIIFBiP animal vivarium in 2019 with a group of piglets of the Irish Landrace breed (n = 10). After slaughter at the age of 210 days, the nuclear (with large tissue particles), mitochondrial and postmitochondrial fractions of the liver were studied with assessment of succinate dehydrogenase and activity of other dehydrogenes of the Krebs cycle. It was found that peroxisomes act as universal agents of communication and cooperation, and microtelets are able to generate various chemical signals that carry information, to control and arrange a number of mechanisms in the metabolic processes in the body. Despite the fact that the Krebs cycle dehydrogenases are considered mitochondrial enzymes, the experiment showed an increase in activity of priruvate dehydrogenase (P > 0.1), isocitrate dehydrogenase (0.1 > P > 0.05) and malate dehydrogenase (0.1 > P > 0.05), which, when comparing the mitochondrial and postmitochondrial fractions, indicates a higher activity of peroxisomal fractions. The peroxisome localization place is the postmitochondrial fraction, and the lower layer contains larger peroxisomes to a greater extent, while the upper layer contains smaller ones. It was found that indicator enzymes of glyoxylate cycle isocitratliase and malate synthase exhibit catalytic activity in the peroxisomal fraction of liver of highly productive pigs. The obtained data on functioning of key glyoxylate cycle enzymes and their intracellular compartmentalization in highly productive pigs allow learning more about the specifics of metabolism and its regulation processes. Application of this knowledge in practice opens up prospects for rationalizing the production of livestock products of increased quantity, improved quality with less feed, labor and financial resources spent.

Objectives. To study the neuroprotective properties of lithium ascorbate (LA) in in vivo and in vitro stress models. Materials and methods. Neurocytological and behavioral studies were run in models of stress in nerve cell cultures and experimental animals. Results. LA was shown to have a marked neuroprotective effect in conditions of glutamate-induced cytotoxicity in vitro and an adaptogenic effect on induction of stress in vivo. Conclusions. The results obtained here demonstrated that LA has high neuroprotective potential in stress induced in vivo and in vitro.

The results of the analysis of comparative studies of neurotrophic drugs based on brain hydrolysates (BH) are presented. The most comprehensive comparative study of the BH drugs carried out by Zhang, et al. 2019 investigated the effects of four drugs (cognistar, cerebrolysate, cortexin, cerebrolysin) on a model of ischemic stroke in rats. The study showed that a significant improvement in the neurological outcome compared with placebo was observed only with cerebrolysin. Higher standardization in elemental composition, higher antioxidant activity, and presence of active peptide fragments of neuropeptides of nerve growth factor, enkephalins, orexin and galanin in cerebrolysin explains neurotrophic and neuroprotective effects of the drug.Представлены результаты анализа сравнительных исследований нейротрофических препаратов на основе гидролизатов головного мозга. Наиболее полным сравнительным исследованием препаратов гидролизатов головного мозга является работа (Zhang, 2019), в которой исследованы эффекты четырех лекарственных препаратов (ЛП) - Когнистар, Церебролизат, Кортексин, Церебролизин - на модели ишемического инсульта у крыс. Результаты исследования продемонстрировали, что значительное улучшение показателей неврологического исхода по сравнению с плацебо отмечено только при использовании ЛП Церебролизин. Более высокая стандартизация по элементному составу, более высокая антиоксидантная активность и наличие активных пептидных фрагментов нейропептидов фактора роста нервов, энкефалинов, орексина, галанина в составе ЛП Церебролизин объясняют его нейротрофическое и нейропротекторное действие.

Investigation of the neuroprotective properties of lithium ascorbate on the stress models in vivo and in vitro.Neurocytological and behavioral studies on nerve cell culture and animal stress models.Significant neuroprotective effect of lithium ascorbate in neuronal cultures exposed to glutamate toxicity and adaptogenic effect of this drug in stress model in rats were shown.The results suggest lithium ascorbate has a high neuroprotective potential in stress models in vivo and in vitro.Цель исследования. Изучение нейропротективных свойств аскорбата лития (АЛ) в моделях стресса in vivo и in vitro. Материал и методы. Нейроцитологические и поведенческие исследования на моделях стресса в культуре нервных клеток и у экспериментальных животных. Результаты. Показан выраженный нейропротективный эффект АЛ при цитотоксическом действии глутамата in vitro и его адаптогенный эффект при индукции стресса in vivo. Заключение. Результаты указывают на высокий нейропротективный потенциал АЛ в условиях стресса, индуцируемого in vivo и in vitro.Цель исследования. Изучение нейропротективных свойств аскорбата лития (АЛ) в моделях стресса in vivo и in vitro. Материал и методы. Нейроцитологические и поведенческие исследования на моделях стресса в культуре нервных клеток и у экспериментальных животных. Результаты. Показан выраженный нейропротективный эффект АЛ при цитотоксическом действии глутамата in vitro и его адаптогенный эффект при индукции стресса in vivo. Заключение. Результаты указывают на высокий нейропротективный потенциал АЛ в условиях стресса, индуцируемого in vivo и in vitro.