23 results on '"Qiao, Chun"'
Search Results
2. List of Contributors
- Author
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Alazem, Mazen, primary, Al-Tuwariqi, Hani Z., additional, Barba, Marina, additional, Boubourakas, Iraklis N., additional, Çağlayan, Kadriye, additional, Candresse, Thierry, additional, Conejero, Vicente, additional, Dadami, Elena, additional, Dalakouras, Athanasios, additional, Daròs, José-Antonio, additional, De la Peña, Marcos, additional, Delgado, Sonia, additional, Di Serio, Francesco, additional, Dixon, Jonathan, additional, Duran-Vila, Nuria, additional, El-Dougdoug, Khaled A., additional, Elleuch, Amine, additional, Faggioli, Francesco, additional, Flores, Ricardo, additional, Fraile, Aurora, additional, Gago-Zachert, Selma, additional, Gallitelli, Donato, additional, García-Arenal, Fernando, additional, Geering, Andrew D.W., additional, Giunchedi, Luciano, additional, Gómez, Gustavo, additional, Gyamera, Ebenezer A., additional, Habili, Nuredin, additional, Hadidi, Ahmed, additional, Hallan, Vipin, additional, Hammond, Rosemarie W., additional, Hanold, Dagmar, additional, Hataya, Tatsuji, additional, Hernández, Carmen, additional, Hong, Ni, additional, Hosakawa, Munetaka, additional, Hsu, Yau-Heiu, additional, Hu, Chung-Chi, additional, Huang, Ying-Wen, additional, James, Delano, additional, Joseph, Hendry, additional, Kalantidis, Kriton, additional, Kaponi, Maria S., additional, Kim, Mikyeong, additional, Kinard, Gary R., additional, Kong, Lih L., additional, Kovalskaya, Natalia, additional, Kuhn, David N., additional, Kyriakopoulou, Panayota E., additional, Lavagi, Irene, additional, Li, Shi-Fang, additional, Lin, Na-Sheng, additional, Ling, Kai-Shu, additional, Liou, Ming-Ru, additional, Lisón, Purificación, additional, López-Carrasco, Amparo, additional, Luigi, Marta, additional, Martínez de Alba, Ángel-Emilio, additional, Mascia, Tiziana, additional, Masuta, Chikara, additional, Matoušek, Jaroslav, additional, Maurel, Marie-Christine, additional, Meekes, Ellis T.M., additional, Minoia, Sofia, additional, Navarro, Beatriz, additional, Nie, Xianzhou, additional, Owens, Robert A., additional, Pallás, Vicente, additional, Palukaitis, Peter, additional, Parakh, Dattaraj B., additional, Perreault, Jean-Pierre, additional, Podleckis, Edward V., additional, Pyle, Jesse D., additional, Randles, John W., additional, Riesner, Detlev, additional, Rodriguez, M. Judith B., additional, Roenhorst, Johanna W., additional, Roossinck, Marilyn J., additional, Rubino, Luisa, additional, Sánchez-Navarro, Jesus A., additional, Sano, Teruo, additional, Scholthof, Karen-Beth G., additional, Serra, Pedro, additional, Shimura, Hanako, additional, Singh, Rudra P., additional, Škorić, Dijana, additional, Stancanelli, Giuseppe, additional, Steger, Gerhard, additional, Taglienti, Anna, additional, Tessitori, Matilde, additional, Thanarajoo, Sathis S., additional, Tiberini, Antonio, additional, Torchetti, Enza M., additional, Tsagris, Mina, additional, Tsushima, Taro, additional, Vadamalai, Ganesan, additional, Verhoeven, Jacobus Th.J., additional, Vidalakis, Georgios, additional, Wang, Qiao-Chun, additional, Wassenegger, Michael, additional, Winter, Stephan, additional, Xu, Wen-Xing, additional, Yang, Xiuling, additional, Zhang, Yongjiang, additional, Zhang, Zhibo, additional, Zhou, Xueping, additional, and Zhu, Shuifang, additional
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- 2017
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3. Cyclodextrin-Based Rotaxanes
- Author
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Tian, He, primary and Wang, Qiao-Chun, additional
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- 2006
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4. List of Contributors
- Author
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Mazen Alazem, Hani Z. Al-Tuwariqi, Marina Barba, Iraklis N. Boubourakas, Kadriye Çağlayan, Thierry Candresse, Vicente Conejero, Elena Dadami, Athanasios Dalakouras, José-Antonio Daròs, Marcos De la Peña, Sonia Delgado, Francesco Di Serio, Jonathan Dixon, Nuria Duran-Vila, Khaled A. El-Dougdoug, Amine Elleuch, Francesco Faggioli, Ricardo Flores, Aurora Fraile, Selma Gago-Zachert, Donato Gallitelli, Fernando García-Arenal, Andrew D.W. Geering, Luciano Giunchedi, Gustavo Gómez, Ebenezer A. Gyamera, Nuredin Habili, Ahmed Hadidi, Vipin Hallan, Rosemarie W. Hammond, Dagmar Hanold, Tatsuji Hataya, Carmen Hernández, Ni Hong, Munetaka Hosakawa, Yau-Heiu Hsu, Chung-Chi Hu, Ying-Wen Huang, Delano James, Hendry Joseph, Kriton Kalantidis, Maria S. Kaponi, Mikyeong Kim, Gary R. Kinard, Lih L. Kong, Natalia Kovalskaya, David N. Kuhn, Panayota E. Kyriakopoulou, Irene Lavagi, Shi-Fang Li, Na-Sheng Lin, Kai-Shu Ling, Ming-Ru Liou, Purificación Lisón, Amparo López-Carrasco, Marta Luigi, Ángel-Emilio Martínez de Alba, Tiziana Mascia, Chikara Masuta, Jaroslav Matoušek, Marie-Christine Maurel, Ellis T.M. Meekes, Sofia Minoia, Beatriz Navarro, Xianzhou Nie, Robert A. Owens, Vicente Pallás, Peter Palukaitis, Dattaraj B. Parakh, Jean-Pierre Perreault, Edward V. Podleckis, Jesse D. Pyle, John W. Randles, Detlev Riesner, M. Judith B. Rodriguez, Johanna W. Roenhorst, Marilyn J. Roossinck, Luisa Rubino, Jesus A. Sánchez-Navarro, Teruo Sano, Karen-Beth G. Scholthof, Pedro Serra, Hanako Shimura, Rudra P. Singh, Dijana Škorić, Giuseppe Stancanelli, Gerhard Steger, Anna Taglienti, Matilde Tessitori, Sathis S. Thanarajoo, Antonio Tiberini, Enza M. Torchetti, Mina Tsagris, Taro Tsushima, Ganesan Vadamalai, Jacobus Th.J. Verhoeven, Georgios Vidalakis, Qiao-Chun Wang, Michael Wassenegger, Stephan Winter, Wen-Xing Xu, Xiuling Yang, Yongjiang Zhang, Zhibo Zhang, Xueping Zhou, and Shuifang Zhu
- Published
- 2017
5. Viroid Elimination by Thermotherapy, Cold Therapy, Tissue Culture, In Vitro Micrografting, or Cryotherapy
- Author
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Zhibo Zhang, Anna Taglienti, Qiao-Chun Wang, Munetaka Hosakawa, and Marina Barba
- Subjects
0106 biological sciences ,Viroid ,viruses ,medicine.medical_treatment ,fungi ,food and beverages ,Cryotherapy ,04 agricultural and veterinary sciences ,Biology ,biology.organism_classification ,01 natural sciences ,Virology ,Cryopreservation ,In vitro ,040501 horticulture ,Tissue culture ,Cold Therapy ,medicine ,Host plants ,0405 other agricultural sciences ,010606 plant biology & botany - Abstract
Viroids cannot be controlled by therapeutic treatments in fields, orchards, vineyards, or palm plantations. Hence, the elimination of viroids from infected plants has been a challenging issue and different approaches have been studied to produce viroid-free planting material. This chapter describes viroid elimination by thermotherapy, cold therapy, tissue culture, in vitro micrografting, or cryotherapy to obtain viroid-free materials. Elevated temperatures do not inactivate or eliminate most viroids from their host plants, whereas cold therapy can be effective depending on the host–viroid system. Meristem culture and shoot-tip micrografting are good methods to obtain viroid-free plants: the eradication rate depends on the size of the shoot, the viroid or its variant, and the host and its variety. The combination of thermotherapy plus shoot-tip culture is more effective for viroid elimination. Cryotherapy of shoot tips, an innovative technique for pathogen eradication based on cryopreservation, did not result in viroid elimination. Further experiments on other host–viroid systems and on combination of cold therapy and cryotherapy are under evaluation.
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- 2017
6. Cyclodextrin-Based Rotaxanes
- Author
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He Tian and Qiao-Chun Wang
- Subjects
chemistry.chemical_classification ,Rotaxane ,Cyclodextrin ,chemistry ,Molecule ,Nanotechnology ,Polymer - Abstract
Publisher Summary This chapter explains the interaction of rotaxanes with CDs. A rotaxane consists of one or more macrocycles encircling the rod portion of a dumb-bell like component. Rotaxanes are generally with some novel chemical and physical properties, and as a result have attracted increasing attention. This chapter provides a summary of the recent works on CD based rotaxanes since the year 2000, including their synthesis and the applications. The types of rotaxanes and their polymers are explained with illustrations. The chapter discusses potential applications of CD based rotaxanes. A rotaxane, which results from wrapping a molecule with a macrocycle, always generates unexpected chemical and physical properties. A great deals of functional materials are then developed with rotaxane like structures. Rotaxane continues to generate plenty of interest due to their potential applications and beautiful structures. Many rotaxanes have been prepared based on CDs, and new concepts keep originated in this area.
- Published
- 2006
7. Vitrification cryo-foil method for shoot tip cryopreservation and virus eradication in apple.
- Author
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Wang MR, Bao JH, Ma XY, Yan ZH, Cui ZH, Zhu LY, Zhang D, and Wang QC
- Abstract
Establishment of a new method for improved shoot tip cryopreservation is crucial to facilitate the long-term preservation of plant germplasm as well as the use of cryotherapy for pathogen eradication. The present study reported a vitrification (V) cryo-foil method for shoot tip cryopreservation and virus eradication in apple. Shoot tip regrowth levels after cryopreservation were comparable among V cryo-foil (53 %), V cryo-plate (46 %) and conventional droplet vitrification (Dr-vi, 48 %). The V cryo-foil is more efficient to perform than Dr-vi as more shoot tips can be cryopreserved by one person. In the histological study applying an image-overlaying strategy, shoot tips cryopreserved by V cryo-foil showed a higher survival chance in the youngest leaf primordia than in the apical dome. When V cryo-foil was tested for virus eradication, fifty-five percent (55 %) of cryo-derived shoots were free of the apple stem pitting virus (ASPV), while none and less than 10 % were free of the apple stem grooving virus (ASGV) and the apple chlorotic leaf spot virus (ACLSV), respectively. Thus, these two viruses were efficiently preserved by V cryo-foil cryopreservation. Noticeably, although the shoot regrowth level was reduced to 27 %, a higher frequency (81 %) of ASPV eradication was achieved when a reduced duration of cryoprotectant exposure was applied in V cryo-foil, supporting the use of insufficient cryoprotection for improved virus eradication., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests and personal relationships that may have influenced the work of this study., (Copyright © 2024 Society for Cryobiology. Published by Elsevier Inc. All rights reserved.)
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- 2024
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8. Significance of changes in the concentration of inflammatory factors in blood or cerebrospinal fluid in evaluating the severity and prognosis of spontaneous cerebral hemorrhage: A systematic review and meta-analysis.
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Xu Y, Ma HY, Qiao CY, Peng Y, Ding Q, Xiang RL, Wang LZ, Yan JF, Hou Y, and Di F
- Abstract
Objective: Spontaneous intracerebral hemorrhage (SICH) often leads to severe disability, while inflammation plays an important role in SICH-induced secondary brain injury. The purpose of this study was to investigate the value of inflammatory factors as a means of evaluating the prognosis of SICH and to investigate the relationship between inflammatory factors and the severity and prognosis of SICH., Methods: The articles published before November 1 2020 were searched through PubMed, EMBASE, Cochrane library and web of science. Revman5.3 was used, using the inverse variance model to pool the SMD of TNF-a and interleukin concentration., Results: A total of 25 studies involving 3333 subjects were included in this paper. The concentration of TNF-α in the blood or cerebrospinal fluid of severe SICH patients was significantly higher than that of milder SICH patients or healthy population; SICH patients with high TNF-α concentration had a 1.06 times greater odds of poor outcomes than patients with low TNF-α concentration, odds ratio (OR) = 1.06[95% CI, 1.01-1.12]. The concentration of interleukin-6 (IL-6) in severe SICH patients was significantly higher than that in milder SICH patients; patients with high IL-6 concentration had a 2.61 times greater odds of poor outcomes than patients with low IL-6 concentration, OR = 2.61[95% CI, 1.79-3.80]., Conclusions: The detection of concentrations of TNF-α and IL-6 in peripheral blood may be helpful for the objective and quantitative assessment of the severity and prognosis of patients with SICH, and have certain significance for the selection of appropriate treatment options., (Copyright © 2021. Published by Elsevier B.V.)
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- 2021
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9. P2X7R orchestrates the progression of murine hepatic fibrosis by making a feedback loop from macrophage to hepatic stellate cells.
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Jiang M, Cui BW, Wu YL, Zhang Y, Shang Y, Liu J, Yang HX, Qiao CY, Zhan ZY, Ye H, Jin Q, Nan JX, and Lian LH
- Subjects
- Animals, Cell Culture Techniques, Cell Line, Tumor, Chemical and Drug Induced Liver Injury pathology, Disease Models, Animal, Extracellular Matrix drug effects, Extracellular Matrix metabolism, Feedback, Physiological, Hepatic Stellate Cells metabolism, Hepatic Stellate Cells pathology, Humans, Liver Cirrhosis pathology, Macrophages, Peritoneal metabolism, Macrophages, Peritoneal pathology, Male, Mice, Mice, Inbred C57BL, Purinergic P2X Receptor Antagonists pharmacology, Pyridines pharmacology, Tetrazoles pharmacology, Thioacetamide toxicity, Chemical and Drug Induced Liver Injury metabolism, Hepatic Stellate Cells drug effects, Liver Cirrhosis metabolism, Macrophages, Peritoneal drug effects, Receptors, Purinergic P2X7 metabolism
- Abstract
HSCs (hepatic stellate cells) contribute to the excessive extracellular matrix (ECM) deposition, inflammatory pathways and crucial cell-cell interactions in hepatic disease leading to fibrosis. P2x7R is considered a potential orchestrater in liver fibrosis. For this reason, this work explored the role of P2x7R in liver fibrosis and the mechanism by which P2x7R in macrophages promotes fibrogenesis. In a model of liver fibrosis induced by administration of thioacetamide (TAA), inhibition of P2x7R with its selective inhibitor A438079 reversed TAA-induced liver damage and fibrosis. The mechanism was linked to inhibition of P2x7R-NLRP3 inflammasome activation and thereby infiltration of macrophages and neutrophils into the liver. This result indicated that the P2x7R-TLR4-NLRP3 axis is involved in the process of TGF-β-mediated ECM deposition in HSCs. Ectopic overexpression of P2x7R lowered the threshold of extracellular matrix (ECM) deposition and maintained HSCs in an activated state. The culture medium of THP-1 macrophages stimulated by LPS/ATP aggravated ECM deposition in HSCs by activating P2x7R. Additionally, IL-1β secreted by LPS / ATP activated macrophages amplified fibrosis. These data indicate that P2x7R plays a key regulative role in the activation and maintenance of HSCs promoted by macrophages. Thus, pharmacological inhibition of P2x7R could be a potential therapeutic mechanism to treat human liver fibrosis., Competing Interests: Declaration of Competing Interest The authors have declared no conflict of interest., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
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10. Cryopreservation of shoot tips, evaluations of vegetative growth, and assessments of genetic and epigenetic changes in cryo-derived plants of Actinidia spp.
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Zhang XC, Bao WW, Zhang AL, Pathirana R, Wang QC, and Liu ZD
- Subjects
- Amplified Fragment Length Polymorphism Analysis, DNA Methylation, Epigenesis, Genetic, Genotype, Vitrification, Actinidia, Cryopreservation methods, Plant Shoots
- Abstract
A droplet-vitrification protocol was described for cryopreservation of shoot tips of kiwifruit 'Yuxiang' (Actinidia chinensis var. deliciosa). No significant differences were found in root formation and shoot growth between the in vitro-derived shoots (the control) and cryo-derived ones when cultured in vitro. No significant differences were detected in survival and vegetative growth between the in vitro-derived plants (the control) and cryo-derived ones after re-establishment in greenhouse conditions. Inter-simple sequence repeat (ISSR) and amplified fragment length polymorphism (AFLP) did not detect any polymorphic bands in the cryo-derived shoots when cultured in vitro and the cryo-derived plants after re-establishment in greenhouse conditions. These data indicate rooting ability, vegetative growth and genetic stability are maintained in the cryo-derived kiwifruit plants recovered from the droplet-vitrification cryopreservation. Methylation sensitive amplification polymorphism (MSAP) detected 12.8% and 1.6% DNA methylation in the cryo-derived shoots when cultured in vitro and the cryo-derived plants after re-established in greenhouse conditions, respectively. This droplet-vitrification was applied to five cultivars and three rootstocks belonging to A. chinensis var. deliciosa, A. chinensis var. chinensis, A. macrosperma, A. polygama and A. valvata. The highest (68.3%) and lowest (22.5%) shoot regrowth were obtained in A. macrosperma and A. chinensis var. chinensis 'Jinmi', respectively, with an average of 46.4% shoot regrowth obtained across the eight genotypes. The droplet-vitrification protocol described here can be considered the most applicable cryopreservation method so far reported for the genus Actinidia. Results reported here provide theoretical and technical supports for setting up cryo-banks of genetic resources of Actinidia spp., Competing Interests: Declaration of competing interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2020
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11. Virus infection reduces shoot proliferation of in vitro stock cultures and ability of cryopreserved shoot tips to regenerate into normal shoots in 'Gala' apple (Malus × domestica).
- Author
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Wang MR, Hao XY, Zhao L, Cui ZH, Volk GM, and Wang QC
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- Flexiviridae, Malus growth & development, Cryopreservation methods, Malus virology, Plant Diseases virology, Plant Shoots growth & development, Plant Shoots virology
- Abstract
Plant cryopreservation has provide secure back-ups of germplasm collections of vegetatively propagated crops. Often, recovery levels vary among laboratories when the same cryogenic procedures are used for the same genotypes. The present study investigated the effects of Apple stem grooving virus (ASGV) on shoot proliferation of in vitro stock cultures and recovery of cryopreserved shoot tips of 'Gala' apple. Results showed that virus infection reduced shoot proliferation of in vitro stock cultures and cell ability to regenerate normal shoots in cryopreserved shoot tips. Virus infection increased total soluble protein, total soluble sugar and free proline levels and altered endogenous levels of indoleacetic acid (IAA) and zeatin riboside (ZR), but induced severe cell membrane damage and caused alternation in mitochondria shape of the in vitro stock shoots. The altered levels of IAA and ZR were most likely to be responsible for the reduced shoot proliferation of in vitro stock culture. Cell damage and alternations in mitochondria shape in ASGV-infected shoot tips were most likely responsible for the reduced cell ability to regenerate normal shoots following cryopreservation. To the best of our knowledge, this is the first study on effects of virus infection on recovery of cryopreserved shoot tips. Results reported here emphasize that healthy in vitro stock cultures should be used for cryopreservation., (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Published
- 2018
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12. Integrative analysis of prognostic factors in Chinese core binding factor leukemia.
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Wang D, Qiao C, Xiao M, Geng Z, Shang Z, He J, Huang M, Yang Y, Zhang N, Liu Y, Li J, Li C, and Zhou J
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- Adolescent, Adult, Aged, Asian People genetics, China epidemiology, Female, Humans, Induction Chemotherapy, Leukemia, Myeloid, Acute therapy, Leukocytes metabolism, Male, Middle Aged, Mutation, Prognosis, Survival Analysis, Young Adult, Core Binding Factors genetics, Leukemia, Myeloid, Acute genetics, Leukemia, Myeloid, Acute mortality, Proto-Oncogene Proteins c-kit genetics
- Abstract
The characteristics of core binding factor (CBF) leukemia appear to differ between Chinese and Caucasian patients. In this study, we analyzed the biological and clinical characteristics of 76 Chinese CBF leukemia patients out of 425 newly diagnosed acute myeloid leukemia (AML) patients. The frequency of CBF AML was 17.9%. Patients harboring t(8;21) were predominant in CBF AML. The incidence of c-kit mutation in CBF AML was 28.9%. The N822K mutation appeared to be more prevalent in Chinese CBF AML patients. Multivariate analysis showed that c-kit mutation and high white blood cell count could negatively impact overall survival (OS) (HR=2.74 and 6.24, P=0.007 and 0.022, respectively) but did not affect relapse-free survival (RFS). Kaplan-Meier analysis showed a significant difference in both OS and RFS between wild-type and mutated c-kit patients. Although we had included recently reported prognostic indicators in our analysis, our results demonstrated that only c-kit mutation and high white blood cell count had prognostic impact on Chinese CBF AML patients., (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Published
- 2012
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13. A new approach for simultaneous screening and quantification of toxic pyrrolizidine alkaloids in some potential pyrrolizidine alkaloid-containing plants by using ultra performance liquid chromatography-tandem quadrupole mass spectrometry.
- Author
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Zhou Y, Li N, Choi FF, Qiao CF, Song JZ, Li SL, Liu X, Cai ZW, Fu PP, Lin G, and Xu HX
- Subjects
- Asteraceae chemistry, Senecio chemistry, Chromatography, Liquid methods, Pyrrolizidine Alkaloids analysis, Tandem Mass Spectrometry methods
- Abstract
A rapid, but sensitive and selective method for simultaneous screening and quantification of toxic pyrrolizidine alkaloids (PAs) by ultra performance liquid-chromatography (UPLC) coupled with tandem mass spectrometry (MS/MS) on a tandem quadrupole mass spectrometer (TQ-MS) is described. This was accomplished by incorporating the precursor ion scan (PIS) acquisition and multiple reaction monitoring (MRM) acquisition in the same UPLC-MS/MS run. Notably, the developed PIS approach for detecting two pairs of characteristic product ions at m/z 120/138 or 168/150, allowed specific identification of toxic retronecine and otonecine types PAs. This PIS method is highly sensitive with over 10-fold sensitivity improvement upon previously published LC-MS method. Moreover, this new approach is suitable for high sample throughput and was applied to the screening and quantifying toxic PAs in 22 samples collected from seven Parasenecio species and four Senecio species. In addition, coupling the MRM with PIS approach generated quantitative results equivalent to those obtained by conventional MRM-only approach. This coupled MRM with PIS approach could provide both qualitative and quantitative results without the need of repetitive analyses., (Copyright © 2010 Elsevier B.V. All rights reserved.)
- Published
- 2010
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14. Qualitative and quantitative analysis of polycyclic polyprenylated acylphloroglucinols from Garcinia species using ultra performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.
- Author
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Zhou Y, Lee S, Choi FF, Xu G, Liu X, Song JZ, Li SL, Qiao CF, and Xu HX
- Subjects
- Isomerism, Plant Extracts chemistry, Chromatography, High Pressure Liquid methods, Garcinia chemistry, Phloroglucinol analysis, Spectrometry, Mass, Electrospray Ionization methods
- Abstract
Polycyclic polyprenylated acylphloroglucinols (PPAPs) are a group of natural products isolated from different Garcinia species with a wide range of important biological activities. In this study, an ultra performance liquid chromatography (UPLC) coupled to photodiode-array detection and quadrupole time-of-flight mass spectrometry (Q-TOF) method was developed to characterize 16 PPAPs in 10 Garcinia species. In source dissociation techniques based on cone voltage fragmentation were used to fragment the deprotonated molecules and multiple mass spectrometry (MS/MS) using ramping collision energy were used to further break down the resulting product ions. The resulting characteristic fragment ions were generated by cleavage of C1-C5 bond and C7-C8 bond through concerted pericyclic reaction, which is especially valuable for differentiating three types of PPAPs isomers. As such, two new PPAPs isomers present in minor amount in the extracts of Garcinia oblongifolia were tentatively characterized by comparing their tandem mass spectra to the known ones. In addition, an UPLC-Q-TOF-MS method was validated for the quantitative determination of PPAPs. The method exhibited limits of detection from 2.7 to 21.4 ng mL(-1) and intra-day and inter-day variations were less than 3.7% and the recovery was in the range of 89-107% with RSD less than 9.0%. This UPLC-Q-TOF-MS method has successfully been applied to quantify 16 PPAPs in 32 samples of 10 Garcinia species, which were found to be a rich source of PPAPs., (Copyright © 2010 Elsevier B.V. All rights reserved.)
- Published
- 2010
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15. An experimental design approach using response surface techniques to obtain optimal liquid chromatography and mass spectrometry conditions to determine the alkaloids in Meconopsi species.
- Author
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Zhou Y, Song JZ, Choi FF, Wu HF, Qiao CF, Ding LS, Gesang SL, and Xu HX
- Subjects
- Alkaloids chemistry, Linear Models, Models, Statistical, Reproducibility of Results, Sensitivity and Specificity, Alkaloids analysis, Chromatography, High Pressure Liquid methods, Papaveraceae chemistry, Spectrometry, Mass, Electrospray Ionization methods
- Abstract
A statistic approach using response surface methodology (RSM) for optimization of the ultra-high performance liquid chromatography (UHPLC) gradient and ionization response of electrospray ionization mass spectrometry (ESI-MS) to analyze the main alkaloids from the plant matrices of six Meconopsi species is presented. The optimization was performed with Box-Behnken designs (BBD) and the multicriteria response variables were described using global Derringer's desirability. Four parameters of UHPLC and six major parameters of ESI-MS were investigated for their contribution to analytes separation and response, leading to a total of 27 and 54 experiments being performed for each instrument, respectively. Quantitative analysis of four main alkaloids in nine samples from six Meconopsis species was employed to evaluate the statistical significance of the parameters on UHPLC-QTOF/ESI-MS analytes response. The results indicated that the optimized UHPLC-QTOF-MS method is very sensitive with the limit of detections (LODs) ranging from 0.5 to 0.1 ng/ml. The overall intra-day and the inter-day variations were less than 2.45%. The recovery of the method was in the range of 94.3-104.8% with RSD less than 4.0%. This approach has important implication in sensitivity enhancement of the ultra-trace determination of alkaloids from complex matrixes in the fields of natural products, metabolomics and pharmacokinetics.
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- 2009
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16. Rapid optimization of dual-mode gradient high performance liquid chromatographic separation of Radix et Rhizoma Salviae Miltiorrhizae by response surface methodology.
- Author
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Song JZ, Qiao CF, Li SL, Zhou Y, Hsieh MT, and Xu HX
- Subjects
- Chromatography, High Pressure Liquid methods, Hydrophobic and Hydrophilic Interactions, Least-Squares Analysis, Models, Theoretical, Reproducibility of Results, Sensitivity and Specificity, Statistics, Nonparametric, Drugs, Chinese Herbal isolation & purification, Rhizome chemistry, Salvia miltiorrhiza chemistry
- Abstract
An approach for rapid optimization of dual-mode gradient high performance liquid chromatography (HPLC) by response surface methodology (RSM) was developed for fast simultaneous separation of hydrophilic and hydrophobic components in Radix et Rhizoma Salviae Miltiorrhizae (Danshen) and its preparations. The aim of this study was to achieve a high throughput RSM optimization using a short ultra-high performance liquid chromatographic (UHPLC) column to simultaneously optimize flow rate and solvent gradient, and then transfer the optimized method to conventional HPLC for routine analytical purposes. The optimization was designed with Box Behnken design (BBD) and the global Derringer's desirability was used for describing the multicriteria response variables. Sixty-two designed experiments were performed by UHPLC with a short sub-2 microm column (2.1 mm x 50 mm, 1.7 microm) and a total running time of only 5h. The predicted gradient profile was further transferred to a long UHPLC column (2.1 mm x 100 mm, 1.7 microm) and a conventional HPLC columns (2.1 mm x 100 mm, 3.5 microm and 4 mm x 100 mm, 5 microm, respectively). Compared to the published methods, the newly developed dual-mode gradient is faster and more efficient at simultaneously separating hydrophilic and hydrophobic components in Danshen and its preparations.
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- 2009
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17. Qualitative and quantitative analysis of diterpenoids in Salvia species by liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.
- Author
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Zhou Y, Xu G, Choi FF, Ding LS, Han QB, Song JZ, Qiao CF, Zhao QS, and Xu HX
- Subjects
- Chromatography, Liquid methods, Diterpenes chemistry, Salvia chemistry, Spectrometry, Mass, Electrospray Ionization methods, Tandem Mass Spectrometry methods
- Abstract
An on-line ultra-high-performance liquid chromatography (UHPLC) coupled with photodiode-array detection and quadrupole time-of-flight tandem mass spectrometry (QTOF-MS/MS) method has been optimized and established for the qualitative and quantitative analysis of the important diterpenoids in the methanol extracts of 12 Salvia species. Specific marker components were identified for the classification of the Salvia samples by principal component analysis. The accurate mass measurement within 3 ppm error for all the protonated molecules and subsequent fragment ions offers higher quality structural information for interpretation of fragmentation pathways of various groups of diterpenoids. Thus, a total of 21 diterpenoids from different Salvia species were separated within 10 min, and were unequivocally or tentatively identified via comparisons with authentic standards and literature. This UHPLC-QTOF-MS/MS method was validated to be sensitive, precise and accurate with the limit of detections at 3.0-16 ng/ml, and the overall intra-day and the inter-day variations less than 3%. The recovery of the method was in the range of 96.2-101.8%, with relative standard deviation (RSD) less than 3.0%. The results demonstrated that the qualitative and quantitative differences in diterpenoids were not only useful for chemotaxonomy in some Salvia species but also for the standardization and differentiation of large numbers of similar samples.
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- 2009
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18. Bioassay guided discovery of apoptosis inducers from gamboge by high-speed counter-current chromatography and high-pressure liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry.
- Author
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Han QB, Zhou Y, Feng C, Xu G, Huang SX, Li SL, Qiao CF, Song JZ, Chang DC, Luo KQ, and Xu HX
- Subjects
- HeLa Cells, Humans, Xanthones isolation & purification, Apoptosis drug effects, Biological Assay, Chromatography, High Pressure Liquid methods, Countercurrent Distribution methods, Garcinia chemistry, Spectrometry, Mass, Electrospray Ionization methods, Xanthones pharmacology
- Abstract
A screening system, composed of high-speed counter-current chromatography and high-pressure liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry, was established to find bioactive lead compound. This system succeeded in discovering apoptosis inducers from gamboge, the resin of Garcinia hanburyi. High-speed counter-current chromatography was used to provide well-separated fractions for bioassay and the resulted active fractions were rapidly identified using high-pressure liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry. The solvent system of n-hexane/ethyl acetate/methanol/water was optimized to the ratio of 7:3:7:3 (v/v/v/v) by a K value analysis. As a result, two active fractions were obtained. They showed apoptosis inducing effects as potent as that of taxol (500 nM) at the concentration of 1 microg/ml. Gambogenic acid (72.1%) and epimeric isogambogic acids (25.3%) were identified in one of the fractions. The other active fraction mainly contained two epimeric mixtures, gambogic acids (68.7%) and gambogoic acids (26.9%). Among them, gambogenic acid, without epimerization, has priority to be lead compound.
- Published
- 2009
- Full Text
- View/download PDF
19. Analysis of caged xanthones from the resin of Garcinia hanburyi using ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry.
- Author
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Zhou Y, Liu X, Yang J, Han QB, Song JZ, Li SL, Qiao CF, Ding LS, and Xu HX
- Subjects
- Chromatography, High Pressure Liquid, Isomerism, Plant Extracts chemistry, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry, Time Factors, Xanthones chemistry, Xanthones isolation & purification, Garcinia chemistry, Resins, Plant chemistry, Xanthones analysis
- Abstract
On-line ultra high-performance liquid chromatography (UHPLC) coupled with electrospray quadrupole time-of-flight tandem mass spectrometry (ESI-QTOF-MS/MS/MS) has been developed for the analysis of a series of caged xanthones in the resin of Garcinia hanburyi. The fragmentation of protonated molecular ions for 12 known cadged xanthones was carried out using low-energy collision-induced electrospray ionization tandem mass spectrometry. It was found that Retro-Diels-Alder rearrangement occurred in the CID processes and produced the characteristic fragment ions, which are especially valuable for the identification of this class of xanthones. The fragmentation differential between some cis-, trans-isomers was uncovered. Computation methods were utilized to rationalize the observed MS behaviors. On-line UHPLC-ESI-MS/MS/MS method has proved to be rapid and efficient in that within 6min, 15 caged scaffold xanthones, including three pairs of epimers and four pairs of isomers in gamboges, were effectively separated and identified. Among them, two known, namely isogambogenin (13) and isomorellinol (14) and one likely new caged Garcinia xanthones from the Garcinia hanburyi were tentatively characterized based on the tandem mass spectra of known ones.
- Published
- 2008
- Full Text
- View/download PDF
20. Characterization of polyprenylated xanthones in Garcinia xipshuanbannaensis using liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.
- Author
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Zhou Y, Han QB, Song JZ, Qiao CF, and Xu HX
- Subjects
- Chromatography, High Pressure Liquid methods, Garcinia chemistry, Spectrometry, Mass, Electrospray Ionization methods, Tandem Mass Spectrometry methods, Xanthones chemistry
- Abstract
A reliable and sensitive on-line high-performance liquid chromatography (HPLC) coupled with electrospray quadrupole time-of-flight tandem mass spectrometry (ESI-QTOF-MS/MS/MS) method has been optimized and established for the analysis of polyprenylated xanthones in the plant Garcinia xipshuanbannaensis. Collision induced MS/MS techniques were used to fragment the precursor molecular ions and MS/MS/MS techniques based on cone voltage fragmentation were used to further break down the resulting product ions sequentially. It was found that Retro-Diels-Alder rearrangement occurred from the xanthone skeleton in the MS/MS/MS process and produced characteristic fragment ions, which are useful for differentiating some positional isomers containing the prenyl unit on the A ring or B ring. Complementary fragmentation information, for instance the successive loss of prenyl residues, is also valuable for the identification of this class of xanthones. Under optimized HPLC-MS/MS/MS method, a total of 15 prenylated xanthones could be separated within 10 min. This method also provided information about the molecular formula of a precursor molecule and its fragments, which could be used for dereplication of known or likely new prenylated xanthones in Garcinia plants before the purification and structural elucidation process.
- Published
- 2008
- Full Text
- View/download PDF
21. Xanthones with growth inhibition against HeLa cells from Garcinia xipshuanbannaensis.
- Author
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Han QB, Yang NY, Tian HL, Qiao CF, Song JZ, Chang DC, Chen SL, Luo KQ, and Xu HX
- Subjects
- Cell Proliferation drug effects, Growth Inhibitors chemistry, HeLa Cells, Humans, Magnetic Resonance Spectroscopy, Molecular Structure, Structure-Activity Relationship, Xanthones chemistry, Garcinia chemistry, Growth Inhibitors pharmacology, Xanthones pharmacology
- Abstract
Eight prenylated xanthones, bannaxanthones A-H (1-8), together with seven known compounds, were isolated from the acetone extract of the twigs of Garcinia xipshuanbannaensis. Their structures were elucidated by spectroscopic data interpretation. The cytotoxic activities of these compounds were evaluated using the MTT method. The results showed that xanthones with an unsaturated prenyl group had stronger cytotoxic activity against cancer cells, whereas those with hydroxylated prenyl groups had none.
- Published
- 2008
- Full Text
- View/download PDF
22. Rapid analysis of pseudolaric acids in Cortex Pseudolaricis and related medicinal products by high performance liquid chromatography.
- Author
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Han QB, Yip YK, Yang NY, Wong L, Qiao CF, Song JZ, Yiu H, and Xu HX
- Abstract
A simple, rapid, reverse-phase high performance liquid chromatographic method was developed for the quantitative analysis of pseudolaric acids in Cortex Pseudolaricis and its related medicinal products. With a C(18) analytical column (4.6mmx150mm i.d.), five pseudolaric acids, namely pseudolaric acids A-C, pseudolaric acid A-O-beta-d-glucopyranoside and pseudolaric acid B-O-beta-d-glucopyranoside, were well separated within 7min. Acetonitrile and 0.10% acetic acid were used as the mobile phase in a gradient program. The UV detection wavelength was set at 260nm. The detection limits and quantification limits ranged in 8.26-16.66ng/ml and 27.54-55.53ng/ml, respectively. The intra- and inter-day variations were less than 1% for all five compounds. The recovery of all spiked pseudolaric acids ranged from 99.1% to 101.9%. Compared to existing analytical methods, this new method not only used two more important chemical markers but also provided a fivefold reduction in analysis time. In addition, the extraction method of herb sample was also modified by an orthogonal array experiment on three variable parameters: extraction time, solvent volume, and extraction cycles. The optimized extraction method was much simpler and could be efficiently used to analyse large set of herbal materials and related medicinal products. Nineteen herb samples collected from different regions of China and five related products were examined with this new analytical method. The results showed that this method is effective in distinguishing adulterants and unqualified products.
- Published
- 2007
- Full Text
- View/download PDF
23. Preparative separation of gambogic acid and its C-2 epimer using recycling high-speed counter-current chromatography.
- Author
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Han QB, Song JZ, Qiao CF, Wong L, and Xu HX
- Subjects
- Stereoisomerism, Countercurrent Distribution methods, Xanthones isolation & purification
- Abstract
A recycling counter-current chromatographic system was first set up with a high-speed counter-current chromatography instrument coupled with a column switching valve. This system was first successfully applied to the preparative separation of epimers, gambogic acid and epigambogic acid from Garcinia hanburyi using n-hexane-methanol-water (5:4:1, v/v/v) as the two-phase solvent system. As a result, 28.2 mg gambogic acid and 18.4 mg epigambogic acid were separated from 50 mg of mixture. Their purities were both above 97% as determined by HPLC. The chemical structures were then identified by their (1)H NMR and (13)C NMR spectra.
- Published
- 2006
- Full Text
- View/download PDF
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