1. CD62L expression level determines the cell fate of myeloid progenitors
- Author
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Mineo Kurokawa, Yusuke Ito, Fumio Nakahara, and Yuki Kagoya
- Subjects
Myeloid ,Neutrophils ,Population ,Cell fate determination ,Biology ,Biochemistry ,Article ,Monocytes ,common myeloid progenitor ,granulocyte-monocyte progenitor ,Gene expression ,Genetics ,medicine ,CD62L ,Animals ,Humans ,Cell Lineage ,L-Selectin ,Progenitor cell ,education ,Myeloid Progenitor Cells ,Progenitor ,education.field_of_study ,Gene Expression Profiling ,Cell Differentiation ,Cell Biology ,Cell biology ,Mice, Inbred C57BL ,Haematopoiesis ,medicine.anatomical_structure ,Gene Expression Regulation ,Monocyte differentiation ,Megakaryocytes ,Developmental Biology - Abstract
Summary Hematopoietic cells differentiate through several progenitors in a hierarchical manner, and recent single-cell analyses have revealed substantial heterogeneity within each progenitor. Although common myeloid progenitors (CMPs) are defined as a multipotent cell population that can differentiate into granulocyte-monocyte progenitors (GMPs) and megakaryocyte-erythrocyte progenitors (MEPs), and GMPs generate neutrophils and monocytes, these myeloid progenitors must contain some lineage-committed progenitors. Through gene expression analysis at single-cell levels, we identified CD62L as a marker to reveal the heterogeneity. We confirmed that CD62L-negative CMPs represent “bona fide” CMPs, whereas CD62L-high CMPs are mostly restricted to GMP potentials both in mice and humans. In addition, we identified CD62L-negative GMPs as the most immature subsets in GMPs and Ly6C+CD62L-intermediate and Ly6C+CD62L-high GMPs are skewed to neutrophil and monocyte differentiation in mice, respectively. Our findings contribute to more profound understanding about the mechanism of myeloid differentiation., Highlights • CD62L-high CMPs are mostly restricted to GMP potentials both in mice and humans • CD62L-neg GMPs are most immature in murine GMPs • Ly6C+/CD62L-int GMPs are skewed to neutrophil differentiation in mice • Ly6C+/CD62L-high GMPs are skewed to monocyte differentiation in mice, Kurokawa and colleagues identify CD62L as a marker to reveal the heterogeneity of common myeloid progenitors (CMPs) and granulocyte-monocyte progenitors (GMPs) through gene expression analysis at single-cell levels. CD62L-high CMPs are mostly restricted to GMP potentials, and Ly6C+/CD62L-int and -high GMPs are skewed to neutrophil and monocyte differentiation, respectively. These findings provide more profound understanding about myeloid differentiation.
- Published
- 2021