1. Immunohistochemical study on the distribution of β-defensin 1 and β-defensin 2 throughout the respiratory tract of healthy rats
- Author
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Youhei Mantani, Nobuhiko Hoshi, Masaya Arai, Hideto Yuasa, Junichi Kawano, Hiroshi Kitagawa, Toshifumi Yokoyama, Miho Nishida, Chiaki Yoshitomi, Wang-Mei Qi, and Natsumi Masuda
- Subjects
0301 basic medicine ,Male ,Pathology ,medicine.medical_specialty ,beta-Defensins ,secretory cell ,030106 microbiology ,Respiratory System ,bactericidal substance ,Mucous membrane of nose ,Bronchi ,Respiratory Mucosa ,Biology ,digestive system ,mucus layer ,Defensins ,03 medical and health sciences ,medicine ,Animals ,Rats, Wistar ,Defensin ,Nose ,respiratory organ ,Bronchus ,General Veterinary ,integumentary system ,Full Paper ,fungi ,Nasal glands ,respiratory system ,bacterial infections and mycoses ,Mucus ,Rats ,Pulmonary Alveoli ,Trachea ,Nasal Mucosa ,030104 developmental biology ,medicine.anatomical_structure ,host defense ,Immunohistochemistry ,Goblet Cells ,Larynx ,Anatomy ,Respiratory tract - Abstract
The distributions of β-defensin 1 and 2 in secretory host defense system throughout respiratory tract of healthy rats were immunohistochemically investigated. In the nasal epithelium, a large number of non-ciliated and non-microvillous cells (NCs) were immunopositive for both β-defensin 1 and 2, whereas a small number of goblet cells (GCs) were immunopositive only for β-defensin 1. Beta-defensin 2-immunopositive GCs were few. In the nasal glands, a small number of acinar cells and a large number of ductal epithelial cells were immunopositive for both β-defensins. In the laryngeal and tracheal epithelia, a very few NCs and GCs were immunopositive for both β-defensins. In laryngeal and tracheal glands, a very few acinar cells and a large number of ductal epithelial cells were immunopositive for both β-defensins. In the extra-pulmonary bronchus, a small number of NCs were immunopositive for both β-defensins. A small number of GCs were immunopositive for β-defensin 1, whereas few GCs were immunopositive for β-defensin 2. From the intra-pulmonary bronchus to alveoli, a very few or no epithelial cells were immunopositive for both β-defensins. In the mucus and periciliary layers, β-defensin 1 was detected from the nose to the extra-pulmonary bronchus, whereas β-defensin 2 was weakly detected only in the nose and the larynx. These findings suggest that the secretory sources of β-defensin 1 and 2 are mainly distributed in the nasal mucosa and gradually decrease toward the caudal airway in healthy rats.
- Published
- 2018