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Purpose: To evaluate the distribution and severity of muscle atrophy in diabetic patients with active Charcot foot (CF) compared to diabetic patients without CF. Furthermore, to correlate the muscle atrophy with severity of CF disease. Material/methods: In this retrospective study, MR images of 35 diabetic patients (21 male, median:62.1 years ± 9.9SD) with active CF were compared with an age- and gender-matched control group of diabetic patients without CF. Two readers evaluated fatty muscle infiltration (Goutallier-classification) in the mid- and hindfoot. Furthermore, muscle trophic (cross-sectional muscle area (CSA)), intramuscular edema (none/mild versus moderate/severe), and the severity of CF disease (Balgrist Score) were assessed. Results: Interreader correlation for fatty infiltration was substantial to almost perfect (kappa-values:0.73–1.0). Frequency of fatty muscle infiltration was high in both groups (CF:97.1–100%; control:77.1–91.4%), but severe infiltration was significantly more frequent in CF patients (p-values: < 0.001–0.043). Muscle edema was also frequently seen in both groups, but significantly more often in the CF group (p-values: < 0.001–0.003). CSAs of hindfoot muscles were significantly smaller in the CF group. For the flexor digitorum brevis muscle, a cutoff value of 139 mm2 (sensitivity:62.9%; specificity:82.9%) in the hindfoot was found to differentiate between CF disease and the control group. No correlation was seen between fatty muscle infiltration and the Balgrist Score. Conclusion: Muscle atrophy and muscle edema are significantly more severe in diabetic patients with CF disease. Muscle atrophy does not correlate with the severity of active CF disease. A CSA < 139 mm2 of the flexor digitorum brevis muscle in the hindfoot may indicate CF disease. [ABSTRACT FROM AUTHOR]

Precipitation events have been predicted and observed to become fewer, but larger, as the atmosphere warms. Water-limited ecosystems are especially sensitive to changes in water cycling, yet evidence suggests that productivity may either increase or decrease in response to precipitation intensification. Interactions among climate, soil properties, and vegetation type may explain different responses, but this is difficult to experimentally test over large spatial scales. Simulation modeling may reveal the mechanisms through which climate, soils, and vegetation interact to affect plant growth. We use an individual-based plant ecohydrological model to simulate the effects of 25%, 50%, and 100% increases in precipitation event sizes on water cycling and shrub, grass, and forb biomass in 200 shrub-steppe sites spanning 651,000 km2 of the Intermountain West, USA. Simulations did not change annual precipitation amounts and were performed for 0, 3, and 5 °C warming. Larger precipitation events decreased evaporation and 'pushed' water into shrub root zones in arid and semi-arid sites, but 'pushed' water below shrub root zones in mesic sites resulting in increased shrub biomass in arid and semi-arid, but not mesic, sites. Positive effects of precipitation intensification on shrub growth partially counteracted negative effects of warming. Grasses and forbs showed no consistent response to precipitation intensification. Results indicate that increased precipitation intensity creates a competitive advantage for shrubs in arid and semi-arid sites. This advantage results in greater shrub relative abundance and suggests that precipitation intensification contributes to woody plant encroachment observed globally in arid and semi-arid ecosystems. [ABSTRACT FROM AUTHOR]

Background and Objective: This systematic review with meta-analysis investigated the influence of resistance training proximity-to-failure on muscle hypertrophy. Methods: Literature searches in the PubMed, SCOPUS and SPORTDiscus databases identified a total of 15 studies that measured muscle hypertrophy (in healthy adults of any age and resistance training experience) and compared resistance training performed to: (A) momentary muscular failure versus non-failure; (B) set failure (defined as anything other than momentary muscular failure) versus non-failure; or (C) different velocity loss thresholds. Results: There was a trivial advantage for resistance training performed to set failure versus non-failure for muscle hypertrophy in studies applying any definition of set failure [effect size=0.19 (95% confidence interval 0.00, 0.37), p=0.045], with no moderating effect of volume load (p=0.884) or relative load (p=0.525). Given the variability in set failure definitions applied across studies, sub-group analyses were conducted and found no advantage for either resistance training performed to momentary muscular failure versus non-failure for muscle hypertrophy [effect size=0.12 (95% confidence interval −0.13, 0.37), p=0.343], or for resistance training performed to high (>25%) versus moderate (20–25%) velocity loss thresholds [effect size=0.08 (95% confidence interval −0.16, 0.32), p=0.529]. Conclusion: Overall, our main findings suggest that (i) there is no evidence to support that resistance training performed to momentary muscular failure is superior to non-failure resistance training for muscle hypertrophy and (ii) higher velocity loss thresholds, and theoretically closer proximities-to-failure do not always elicit greater muscle hypertrophy. As such, these results provide evidence for a potential non-linear relationship between proximity-to-failure and muscle hypertrophy. [ABSTRACT FROM AUTHOR]

Portland cement-based grouts used for radioactive waste immobilisation contain a Ca- and Si-rich binder phase, known as calcium–silicate–hydrate (C–S–H). Depending on the blend of cement used, the Ca/Si ratio can vary considerably. A range of C–S–H minerals with Ca/Si ratios from 0.6 to 1.6 were synthesised and contacted with aqueous U(VI) at 0.5 mM and 10 mM concentrations. Solid-state 29Si MAS-NMR spectroscopy was applied to probe the Si coordination environment in U(VI)-contacted C–S–H minerals and, in conjunction with U LIII-edge X-ray absorption spectroscopy analysis, inferences of the fate of U(VI) in these systems were made. At moderate or high Ca/Si ratios, uranophane-type uranyl silicates or Ca-uranates dominated, while at the lowest Ca/Si ratios, the formation of a Ca-bearing uranyl silicate mineral, similar to haiweeite (Ca[(UO2)2Si5O12(OH)2]·3H2O) or Ca-bearing weeksite (Ca2(UO2)2Si6O15·10H2O) was identified. This study highlights the influence of Ca/Si ratio on uranyl sequestration, of interest in the development of post-closure safety models for U-bearing radioactive waste disposal. [ABSTRACT FROM AUTHOR]

Introduction: Repetitive minor amputations carry the concomitant risks of multiple surgical procedures, major amputations have physical and economical major drawbacks. The aim of this study was to evaluate whether there is a distinct number of minor amputations predicting a major amputation in the same leg and to determine risk factors for major amputation in multiple minor amputations. Materials and methods: A retrospective chart review including 429 patients with 534 index minor amputations between 07/1984 and 06/2019 was conducted. Patient demographics and clinical data including number and level of re-amputations were extracted from medical records and statistically analyzed. Results: 290 legs (54.3%) had one or multiple re-amputations after index minor amputation. 89 (16.7%) legs needed major amputation during follow up. Major amputation was performed at a mean of 32.5 (range 0 – 275.2) months after index minor amputation. No particular re-amputation demonstrated statistically significant elevated odds ratio (a.) to be a major amputation compared to the preceding amputation and (b.) to lead to a major amputation at any point during follow up. Stepwise multivariate Cox regression analysis revealed minor re-amputation within 90 days (HR 3.8, 95% CI 2.0-7.3, p <0.001) as the only risk factor for major amputation if at least one re-amputation had to be performed. Conclusions: There is no distinct number of prior minor amputations in one leg that would justify a major amputation on its own. If a re-amputation has to be done, the timepoint needs to be considered as re-amputations within 90 days carry a fourfold risk for major amputation. Level of evidence: Retrospective comparative study (Level III). [ABSTRACT FROM AUTHOR]

Hyperspectral remote sensing already is important in geoscientific research in the fields of geology, soil, exploration and mining. New hyperspectral satellite systems are already in operation (e.g. PRISMA and DESIS; Caporusso et al. 2020; Alonso et al. (Sensors 19(20):4471–4515, 2019)) and more systems are planned e.g. the European Copernicus Next Generation Hyperspectral Satellite CHIME (Nieke and Rast 2018). The German system EnMAP was successfully launched into space on 1st of April 2022 (DLR 2022). The potential of hyperspectral airborne and satellite borne data for mining-related applications is discussed. Investigated are the information contents of hyperspectral data for exploration target recognition and their dependency on spatial resolutions of different sensor platforms. Airborne data offer high spatial resolution of 2.5 m with limited areal data acquisition, whereas hyperspectral spaceborne sensors guaranty nearly worldwide data availability with the same spectral characteristics but medium spatial resolution (30 m). The aspects of high spectral resolution and high versus medium spatial resolution targeted mineral mapping is investigated. The methodological concept includes processing aspects, standardized data availability for mineral mapping and mineralization targeting for operational application, to maintain/allow application of hyperspectral data even for non-remote sensing experts. Based on hyperspectral airborne data acquired in the Aggeneys region in South Africa, spaceborne hyperspectral data are simulated following the EnMAP specifications, and the concept for targeted mapping of surface alterations of a lead zinc deposit is discussed. [ABSTRACT FROM AUTHOR]

Osteoarthritis (OA) is a common disabling disease worldwide, with no effective and safe disease-modifying drugs (DMOAD) in the market. However, studies suggest that drugs, such as liraglutide, which possess strong potential in decreasing low-grade systemic inflammation may be effective in treating OA. Therefore, the aim of this study was to examine the anti-inflammatory, analgesic, and anti-degradative effects in OA using in vitro and in vivo experiments. The results showed that intra-articular injection of liraglutide alleviated pain-related behavior in in vivo sodium monoiodoacetate OA mouse model, which was probably driven by the GLP-1R-mediated anti-inflammatory activity of liraglutide. Moreover, liraglutide treatment significantly decreased IL-6, PGE2 and nitric oxide secretion, and the expression of inflammatory genes in vitro in chondrocytes and macrophages in a dose-dependent manner. Additionally, liraglutide shifted polarized macrophage phenotype in vitro from the pro-inflammatory M1 phenotype to the M2 anti-inflammatory phenotype. Furthermore, liraglutide exerted anti-catabolic activity by significantly decreasing the activities of metalloproteinases and aggrecanases, a family of catabolic enzymes involved in cartilage breakdown in vitro. Overall, the findings of this study showed that liraglutide ameliorated OA-associated pain, possess anti-inflammatory and analgesic properties, and could constitute a novel therapeutic candidate for OA treatment. [ABSTRACT FROM AUTHOR]

Muscarinic receptor antagonists and the β3-agonist mirabegron form the basis of the drug treatment of the overactive bladder syndrome (OAB). The efficacy of muscarinic receptor antagonists and mirabegron are comparable and the combination of solifenacin and mirabegron is moderately more effective than either monotherapy. The side effects of antimuscarinics include dry mouth and constipation and those of mirabegron are similar to placebo. Few patients use these drugs for a longer time due to lack of efficacy and side-effects. No potential drugs with new mechanisms of action seem to be in clinical trials at the moment and there is little chance that this will change in the short term. Because OAB is a symptom complex, with possibly several underlying pathologies and various combinations of symptoms, it is unlikely that new agents will be significantly more effective. The main priorities for the future are not only the discovery of new therapeutic agents, but also identification of subgroups with the same symptomatology or underlying pathology. Biomarkers could play a role in this search. [ABSTRACT FROM AUTHOR]

We show that there are one-loop IR singularities arising from UV/IR mixing in noncommutative SU(N) Yang–Mills theory defined by means of the θ -exact Seiberg–Witten map. This is in spite of the fact that there are no ordinary U(1) gauge fields in the theory and this is at variance with the noncommutative U(N) case, where the two-point part of the effective action involving the ordinary SU(N) fields do not suffer from those one-loop IR singularities. [ABSTRACT FROM AUTHOR]

Recreational fishing is practiced by ~ 350 million people globally, and while it historically has been thought to have minimal ecological impact relative to commercial fishing, numerous recreational fisheries have recently declined or collapsed. The potential for recreational fishing to contribute to ecological decline, as well as the incentives of recreational anglers that are distinct from those of commercial fishers, highlights the need for greater understanding of recreational fisheries regulatory options. To aid managers in the decision-making process, we conduct the first comparative review of all seven major approaches to recreational fisheries regulation: harvest size restrictions, harvest quantity restrictions, spatial management, temporal restrictions, accessibility restrictions, rights-based management, and gear restrictions. We provide a synthetic guide for students and practitioners covering how these regulations can benefit target stocks, their potential limitations in achieving sustainability, and angler perceptions of their relative effectiveness and behavioral impositions. Considering the strengths and weaknesses of each strategy, we identify three key fishery metrics that together can guide selection of a suitable combination of regulations that will achieve the requisite biological outcome without restricting angler behavior more than is necessary. With this perspective, we reflect on uncertainties that complicate informed and effective, recreational fisheries regulation. [ABSTRACT FROM AUTHOR]

The aim of this study was to compare two different techniques of performing one-level spondylodesis for thoracolumbar burst fractures using either an autologous iliac crest bone graft (ICBG) or a porous tantalum fusion implant (PTFI). In a prospective nonrandomized study, 44 patients (20 women, 24 men; average age 43.1 ± 13.2 years) suffering from severe thoracolumbar burst fractures were treated with combined anterior–posterior stabilization. An ICBG was used in 21 cases, and a PTFI was used in the other 23 cases. A two-year clinical and radiographic follow-up was carried out. There were no statistically significant differences in age, sex, localization/classification of the fracture, or visual analog scale (VAS) before injury between the two groups. All 44 patients were followed up for an average period of 533 days (range 173–1567). The sagittal spinal profile was restored by an average of 11.1° (ICBG) vs. 14.3° (PTFI) (monosegmental Cobb angle). Loss of correction until the last follow-up tended to be higher in the patients treated with ICBG than in those treated with PTFI (mean: 2.8° vs. 1.6°). Furthermore, significantly better restoration of the sagittal profile was obtained with the PTFI than with the iliac bone graft at the long-term follow-up (mean: ICBG 7.8°, PTFI 12.3°; p < 0.005). Short-segment posterior instrumentation combined with anterior one-level spondylodesis using either an ICBG or a PTFI resulted in sufficient correction of posttraumatic segmental kyphosis. PTFI might be a good alternative for autologous bone grafting and prevent donor site morbidities. [ABSTRACT FROM AUTHOR]

Operative treatment of ruptured pubic symphysis by plating is often accompanied by complications. Trans-obturator cable fixation might be a more reliable technique; however, have not yet been tested for stabilization of ruptured pubic symphysis. This study compares symphyseal trans-obturator cable fixation versus plating through biomechanical testing and evaluates safety in a cadaver experiment. APC type II injuries were generated in synthetic pelvic models and subsequently separated into three different groups. The anterior pelvic ring was fixed using a four-hole steel plate in Group A, a stainless steel cable in Group B, and a titan band in Group C. Biomechanical testing was conducted by a single-leg-stance model using a material testing machine under physiological load levels. A cadaver study was carried out to analyze the trans-obturator surgical approach. Peak-to-peak displacement, total displacement, plastic deformation and stiffness revealed a tendency for higher stability for trans-obturator cable/band fixation but no statistical difference to plating was detected. The cadaver study revealed a safe zone for cable passage with sufficient distance to the obturator canal. Trans-obturator cable fixation has the potential to become an alternative for symphyseal fixation with less complications. [ABSTRACT FROM AUTHOR]

Gastrointestinal symptoms (GIS) are common in kidney transplant candidates and recipients and may be worsened by HIV. Objective: To determine the frequency and severity of GIS in HIV-positive kidney transplant recipients from HIV-positive donors, and those waiting to receive one. A GIS rating scale (GSRS) was completed by 76 participants at baseline and at 6 months. GIS frequency was defined as having at least one symptom (GSRS > 1). Severity was indicated by the GSRS score. Transplant candidates: GIS frequency was 88.9% and 86.3% at baseline and 6 months respectively. Indigestion was the most frequent (79.6% and 66.7% at baseline and 6 months), and severe GIS (GSRS 2.3). Women reported global mean (p = 0.030) severity significantly more than men. Transplant recipients: GIS frequency was 95.2% and 76.2% at baseline and 6 months respectively. At both assessment points, indigestion occurred most frequently (85.7% and 61.9% respectively). Highest GSRS was reported for indigestion at baseline (2.33) and at 6 months (1.33). Waist circumference (WC) was positively associated with the severity of constipation GSRS. GIS are common in both groups, especially indigestions. WC in transplant recipients should be monitored. [ABSTRACT FROM AUTHOR]

Uranyl oxalate (UO2C2O4·xH2O) may exist at the back-end of the nuclear fuel cycle (NFC) as an intermediate in spent fuel reprocessing. The conditions used in aqueous reprocessing and thermal treatment can affect the physical and chemical properties of the material. Furthermore, trace impurities, such as Fe, may incorporate into the structure of these materials. In nuclear forensics, understanding relationships between processing variables aids in determination of provenance and processing history. In this study, the thermal decomposition of UO2C2O4·3H2O and phase analysis of its thermal products are examined. Their morphologies are discussed with respect to a matrix of solution processing conditions. [ABSTRACT FROM AUTHOR]

Yeast and cancer cells are metabolically similar as they use fermentation of glucose as a primary means of generating energy. Reliance on glucose fermentation makes both of these cell types highly sensitive to the toxic glucose analog, 2-deoxyglucose. Here we review the cellular and metabolic pathways that play a role in 2-deoxyglucose sensitivity and discuss how the modifications to these pathways result in acquisition of 2-deoxyglucose resistance. Insights gained from genetic and proteomic studies in yeast provide new ideas for the design of combinatorial therapies for cancer treatment. [ABSTRACT FROM AUTHOR]

Objective: To develop a new magnetic resonance imaging(MRI) scoring system for evaluation of active Charcot foot and to correlate the score with a duration of off-loading treatment ≥ 90 days. Methods: An outpatient clinic database was searched retrospectively for MRIs of patients with active Charcot foot who completed off-loading treatment. Images were assessed by two radiologists (readers 1 and 2) and an orthopedic surgeon (reader 3). Sanders/Frykberg regions I–V were evaluated for soft tissue edema, bone marrow edema, erosions, subchondral cysts, joint destruction, fractures, and overall regional manifestation using a score according to degree of severity (0–3 points). Intraclass correlations (ICC) for interreader agreement and receiver operating characteristic analysis between MR findings and duration of off-loading-treatment were calculated. Results: Sixty-five feet in 56 patients (34 men) with a mean age of 62.4 years (range: 44.5–85.5) were included. Region III (reader 1/reader 2: 93.6/90.8%) and region II (92.3/90.8%) were most affected. The most common findings in all regions were soft tissue edema and bone marrow edema. Mean time between MRI and cessation of off-loading-treatment was 150 days (range: 21–405). The Balgrist Score was defined in regions II and III using soft tissue edema, bone marrow edema, joint destruction, and fracture. Interreader agreement for Balgrist Score was excellent: readers 1/2: ICC 0.968 (95% CI: 0.948, 0.980); readers 1/2/3: ICC 0.856 (0.742, 0.917). A cutoff of ≥ 9.0 points in Balgrist Score (specificity 72%, sensitivity 66%) indicated a duration of off-loading treatment ≥ 90 days. Conclusion: The Balgrist Score is a new MR scoring system for assessment of active Charcot foot with excellent interreader agreement. The Balgrist Score can help to identify patients with off-loading treatment ≥ 90 days. [ABSTRACT FROM AUTHOR]

ThTi2O6 derived compounds with the brannerite structure were designed, synthesised, and characterised with the aim of stabilising incorporation of U5+ or U6+, at dilute concentration. Appropriate charge compensation was targeted by co-substitution of Gd3+, Ca2+, Al3+, or Cr3+, on the Th or Ti site. U L3 edge X-ray Absorption Near Edge Spectroscopy (XANES) and High Energy Resolution Fluorescence Detected U M4 edge XANES evidenced U5+ as the major oxidation state in all compounds, with a minor fraction of U6+ (2–13%). The balance of X-ray and Raman spectroscopy data support uranate, rather than uranyl, as the dominant U6+ speciation in the reported brannerites. It is considered that the U6+ concentration was limited by unfavourable electrostatic repulsion arising from substitution in the octahedral Th or Ti sites, which share two or three edges, respectively, with neighbouring polyhedra in the brannerite structure. [ABSTRACT FROM AUTHOR]

Background: Randomized clinical trials have demonstrated efficacy and safety of erenumab. The aim of this study is to evaluate the effectiveness and safety of erenumab in a real-world setting in French patients with migraine associated with extreme unmet needs. Methods: This is a one year-prospective real-word study with enrolment of all consecutive adult patients included in the FHU InovPain registry who participated in a compassionate erenumab use program. Results: Of 144 patients included, 140 patients (82.1% female / mean age of 50.9 ± 11.4) received at least one dose of erenumab and were concerned by effectiveness and safety assessment. All patients had failed 11 oral preventive treatments. Most of them suffered from chronic migraine (88.6%) and presented a medication overuse (90.7%) at baseline. Thirty-eight (27.1%) discontinued treatment during the 12-month follow-up, with 22 (15.7%), 11 (7.9%) and 5 (3.6%) patients before 3, 6 or 9 months of treatment. The proportion of ≥ 50% responders at M3, M6, M9 and M12 was 74/140 (52.9%), 69/118 (58.5%), 61/107 (57.0%) and 60/102 (58.8%) respectively. At M3, the rate of reversion from chronic migraine to episodic migraine was 57.3% and the rate of transition from medication overuse to non-overuse was 46.5%. For monthly migraine days, the median (IQR) was 18.0 (13.0–26.0), 9.0 (5.0–17.0), 7.5 (5.0–14.0), 8.0 (5.0–12.5) and 8.0 (5.0–12.0) at M0, M3, M6, M9 and M12 respectively. For HIT-6 score, the median (IQR) was 68.0 (63.8–73.3), 60.0 (54.0–65.0), 60.0 (50.3–53.0), 59.0 (50.0–63.0) and 58.0 (50.0–62.9) at M0, M3, M6, M9 and M12 respectively. Fifty-three (37.9%) patients reported at least one of the following adverse events: cutaneous erythema and/or pain at the injection site for 42 (30%) patients, constipation for 22 (15.7%) patients, muscle spasm for 2 (1.4%) patients, alopecia for one (0.7%) patient and blood pressure increase in one (0.7%) patient. There was no serious adverse event. One female patient became pregnant after 5 months of exposure to erenumab with a safe evolution after treatment discontinuation. Conclusion: This first French real-world study related to migraine prevention with CGRP-mAbs confirms effectiveness and safety of erenumab in patients with extreme unmet needs. [ABSTRACT FROM AUTHOR]

The binary manganese–rhodium (Mn–Rh) phase diagram was reinvestigated from 5 to 90 at.% Rh with focus on determining the transition temperature between the ordered γ′-Mn3Rh and the γ-Mn phase as well as the transition temperature between of the tetragonal and cubic MnRh phase and phase boundaries, applying XRD, DTA and SEM including EDX. A reassessment of the Mn–Rh phase diagram based on obtained and literature data is given. Furthermore, the phase equilibria of the ternary bismuth–manganese–rhodium (Bi–Mn–Rh) system were experimentally investigated, focusing on the possible existence of new ferromagnetic phases. Isothermal sections at 330 °C and 600 °C were studied applying powder XRD and EDX. The corresponding phase diagram was established based on these results. No additional ferromagnetic phase was found in the ternary system. [ABSTRACT FROM AUTHOR]

Background: A high percentage of menopausal and perimenopausal women suffer symptoms that deteriorate their quality of life (QoL) significantly. Many studies have focused on the relationship between perimenopausal symptoms and QoL, yet the results obtained have been inconclusive. The aim of this study is to evaluate the relationships among the symptoms of menopause, sociodemographic variables, knowledge of menopause and QoL.Method: Sociodemographic and clinical data was collected from interviews of 453 women in Madrid, and they also completed questionnaires related to perimenopausal symptomatology (MRS, MENQOL), knowledge of menopause and QoL.Results: Although dependent on the assessment techniques, all the tools used indicated that more than half of the women studied suffered perimenopausal symptomatology: interview (59.1%), MENQOL (69.2%) and MRS (65.1%). Stronger symptoms were related to a worse QoL (R2 = 0.287 for MENQOL; R2 = 0.390 for MRS), being psychosocial/psychological and urogenital/sexual symptomatology, and educational level and knowledge about menopause the most strongly related to this parameter. Taking into account the main perimenopausal symptoms in Europe, psychosocial and sexual symptoms are also found to be strongly related to QoL.Conclusion: Perimenopausal symptomatology is frequent and intense, deteriorating women's QoL. While psychosocial and somatic/physical symptoms are the most frequent and intense, psychosocial/psychological and urogenital/sexual are those that best predict the individual's QoL. Educational level and knowledge about menopause are also related to a better QoL. [ABSTRACT FROM AUTHOR]

Purpose: Malignancies of the vagina are rare, but colposcopy-directed biopsies play a major role in detecting vaginal intraepithelial lesions. Data of accuracy in detecting neoplasia of the vagina are very rare compared to accuracy in detecting cervical neoplasia. The aim of this study was to evaluate the accuracy of colposcopy-directed biopsy in comparison with clinical findings of the examiner.Methods: The accuracy of colposcopy-directed biopsy was compared with the clinical finding in relation to the patient's age and the examiner's level of training. This was done in combination with PAP-smear, HPV-test results, and the history of other malignancies of the lower genital tract. The data were collected between January 2014 and February 2018 at the certified Dysplasia Unit of the University Hospital Erlangen.Results: In total, 253 biopsies from 253 women from the vagina were obtained. The overall accuracy of biopsy in comparison with clinical finding was 52.17% for all entities-benign lesions, low-grade squamous intraepithelial lesions (LSILs), high-grade squamous intraepithelial lesions (HSILs), and vaginal carcinoma. The accuracy for detecting HSIL was 82.46% (47/57), with an underdiagnosis rate of 15.79% and an overdiagnosis rate of 1.79%.Conclusion: With a sensitivity of over 80%, colposcopy-directed biopsy plays an important role in detecting vaginal-HSIL. A highly experienced practitioner is increasing the sensitivity in detecting vaginal-HSIL. Careful examination is required in women with a history of HSIL of the lower genital tract or with simultaneous neoplasia because they are of greater risk of developing vaginal malignancies. The combination of careful clinical work up, PAP-smear, HPV-testing, and colposcopy-guided biopsy is crucial in detecting vaginal-HSIL. [ABSTRACT FROM AUTHOR]

Osteosarcoma (OS) is the most common bone cancer in children and young adults. Solid tumors are characterized by intratumoral hypoxia, and hypoxic cells are associated with the transformation to aggressive phenotype and metastasis. The proteome needed to support an aggressive osteosarcoma cell phenotype remains largely undefined. To link metastatic propensity to a hypoxia-induced proteotype, we compared the protein profiles of two isogenic canine OS cell lines, POS (low metastatic) and HMPOS (highly metastatic), under normoxia and hypoxia. Label-free shotgun proteomics was applied to comprehensively characterize the hypoxia-responsive proteome profiles in the OS cell phenotypes. Hypothesis-driven parallel reaction monitoring was used to validate the differential proteins observed in the shotgun data and to monitor proteins of which we expected to exhibit hypoxia responsiveness, but which were absent in the label-free shotgun data. We established a "distance" score (|zHMPOS − zPOS|), and "sensitivity" score (|zHypoxia − zNormoxia) to quantitatively evaluate the proteome shifts exhibited by OS cells in response to hypoxia. Evaluation of the sensitivity scores for the proteome shifts observed and principal component analysis of the hypoxia-responsive proteins indicated that both cell types acquire a proteome that supports a Warburg phenotype with enhanced cell migration and proliferation characteristics. Cell migration and glucose uptake assays combined with protein function inhibitor studies provided further support that hypoxia-driven adaption of pathways associated with glycolytic metabolism, collagen biosynthesis and remodeling, redox regulation and immunomodulatory proteins typify a proteotype associated with an aggressive cancer cell phenotype. Our findings further suggest that proteins involved in collagen remodeling and immune editing may warrant further evaluation as potential targets for anti-metastatic treatment strategies in osteosarcoma. [ABSTRACT FROM AUTHOR]

1 HT ht 3 HT ht 5 HT ht 11 HT ht. [Extracted from the article]

Carbon isotopes are commonly used in trophic ecology to estimate consumer diet composition. This estimation is complicated by the fact that lipids exhibit a more depleted carbon signature (δ13C) than other macromolecules, and are often found at different concentrations among individual organisms. Some researchers argue that lipids bias diet reconstructions using stable isotopes and should be accounted for prior to analysis in food web mixing models, whereas others contend that removing lipids may result in erroneous interpretations of the trophic interactions under study. To highlight this disagreement on best practices for applying δ13C in food web studies, we sampled the recent literature to determine the frequency and method of lipid-correction. We then quantified the potential magnitude and source of bias in mixing model results from a theoretical example and case study of diet reconstruction. The literature was split nearly evenly as to whether lipid-correction was applied to δ13C data in mixing model estimates of diet composition. Comparative mixing model scenarios demonstrated that lipid-correction can substantially alter the estimated diet composition and interpretation of consumer foraging habits. Given the lack of consensus on whether or not to lipid-correct prey and/or consumers, and the associated variation in mixing model results, we call for the establishment of a unified framework that will guide diet reconstruction in stable isotope ecology. Uncertainty in the prevalence of direct routing versus de novo synthesis of lipids across ecosystems, taxa, and trophic levels must be resolved to better guide treatment of lipids in isotope studies using carbon. [ABSTRACT FROM AUTHOR]

Since a wise man or woman proportions his or her belief to the evidence [[1]], this focus on paediatrics discusses interesting and influential papers that have been published in important journals in 2018, related to paediatric intensive care unit (PICU) clinical research. The pilot trials for conservative versus liberal oxygenation targets in critically ill children (oxy-PICU) [[17]] and high flow nasal cannula oxygen versus CPAP (first ABC) [[18]], represent "for runners" to multi centre RCT starting in 2019. Overview of studies in critically ill children (search terms child, critical care OR intensive care) that are currently recruiting. 18 Ramnarayan P, Lister P, Dominguez T, Habibi P, Edmonds N, Canter RR, Wulff J, Harrison DA, Mouncey PM, Peters MJ United Kingdom Paediatric Intensive Care Society Study G. FIRST-line support for Assistance in Breathing in Children (FIRST-ABC): a multicentre pilot randomised controlled trial of high-flow nasal cannula therapy versus continuous positive airway pressure in paediatric critical care. [Extracted from the article]

Natural variability of rock properties can significantly affect the strength of rock masses and factor of safety of slopes. The results of a comprehensive point load testing program showed that coefficient of variation of intact rock strength can reach unity in highly heterogeneous formations. Probabilistic numerical analysis was carried out to explore the effect of strength variability on uniaxial compressive strength of large heterogeneous samples. It was shown that mean large-scale strength decreases with increasing small-scale variability. The effect of spatial variability of strength properties on slope stability was examined using limit equilibrium and shear strength reduction methods. Both approaches gave similar results indicating that for stable slopes, increasing strength variability leads to a reduction in mean factor of safety and increase in the probability of failure. In addition, ignoring spatial variability in probabilistic slope analysis can lead to erroneous estimates of the probability of failure. Based on the results of probabilistic analyses on large heterogeneous samples and slopes, an equivalent uniaxial compressive strength can be obtained by reducing the mean strength by one-third of its standard deviation. This relationship was validated using a dataset of back-analyzed strength values in heterogeneous open pit slopes. [ABSTRACT FROM AUTHOR]

This paper is concerned with the construction of a new exact solution to the geophysical fluid dynamics governing equations for inviscid and incompressible fluid in the equatorial region. This solution represents a steady purely-azimuthal flow with a free-surface. The novel aspect of the solution we derive is that the flow it prescribes accommodates a general fluid stratification: the density may vary both with depth, and with latitude. The solution is presented in the terms of spherical coordinates, hence at no stage do we invoke approximations by way of simplifying the geometry in the governing equations. Following the construction of our explicit solution, we employ functional analytic considerations to prove that the pressure at the free-surface defines implicitly the shape of the free-surface distortion in a unique way, exhibiting also the expected monotonicity properties. Finally, using a short-wavelength stability analysis we prove that certain flows defined by our exact solution are stable for a specific choice of the density distribution. [ABSTRACT FROM AUTHOR]

Rock fall hazard management typically requires efficient allocation of available resources for detailed hazard assessment and control. In mountainous terrain, remoteness and difficult access pose a challenge for resource allocation decision-making. In this paper, we evaluate a systematic approach for decision-making based on topographic susceptibility and efficient three-dimensional trajectory modelling. The approach relies on the availability of detailed digital elevation models, a strategy which is now considered to be the state of practice. In this study, it is assumed that geologic and rock mass quality information, as well as rock fall occurrence records, are not available in the necessary detail across the area of study for them to be used in rock fall hazard assessments. The approach is illustrated with a case study along a section of railway within the Canadian Cordillera. The advantages and limitations of the approach are discussed, as well as some key considerations for its application. [ABSTRACT FROM AUTHOR]

The phosphorylation status of a protein is highly regulated and is determined by the opposing activities of protein kinases and protein phosphatases within the cell. While much is known about the protein kinases found in Saccharomyces cerevisiae, the protein phosphatases are much less characterized. Of the 127 protein kinases in yeast, over 90% are in the same evolutionary lineage. In contrast, protein phosphatases are fewer in number (only 43 have been identified in yeast) and comprise multiple, distinct evolutionary lineages. Here we review the protein phosphatase families of yeast with regard to structure, catalytic mechanism, regulation, and signal transduction participation. [ABSTRACT FROM AUTHOR]

A failure envelope that ignores the intermediate principal stress is typically adequate for the design of rock structures. To obtain the complete envelope, the rock cylinders should be tested using confined compression as well as confined extension tests. While confined compression tests are common, confined extension tests are rarely carried out. Current techniques available to test rocks under confined extension conditions require shaping the cylindrical specimens to a dog-bone geometry. The limited data available in the literature indicates that the results from these dog-boned shaped tests produces strengths that are considerably greater than the confined triaxial tests carried out on traditional cylindrical specimens. Whether this increased strength is real or simply an artifact of the stress path for triaxial confined extension tests (intermediate principal stress equal to the maximum principal stress) versus confined compression tests (intermediate principal stress equal to the minimum principal stress) is unknown. We propose here a new methodology to test rocks under confined extension condition with zero intermediate principal stress. We used the flattened Brazilian test and calculated the major and minor principal stresses from the strain on the surface of the specimens. To test the rock under increased confined conditions, we tested specimens with increasing depth of flattening. We derived equations to calculate the major and minor principal stresses in a flattened Brazilian specimen considering the bi-modularity in the stress-strain equations. We tested Lac du Bonnet granite specimens for major principal stress range 15-37% of its UCS and observed that the minor principal stresses remains in the range of Brazilian strength of the material (11.6 MPa). The results suggest that the nonlinear Hoek-Brown failure criterion based on only compressive triaxial results provides a reasonable estimate of the tensile strength. [ABSTRACT FROM AUTHOR]

Homogeneous goods markets with convex costs, do not generally possess Bertrand-Nash equilibria in pure strategies. In order to identify ex-post stable prices in such markets, the set of outcomes feasible in Bertrand competition are analysed as a non-transferable utility coalitional game. The market-clearing price is shown to always implement a strict-core outcome. Moreover, where at least two sellers compete, the strict-core converges to only admit market-clearing outcomes. The analysis has implications for a number of prominent models of oligopoly competition. When firms engage in capacity pre-commitment, the set of ex-post stable prices converges to the corresponding Cournot prices. This result holds for arbitrary capacity choices and a general class of rationing rules. Conversely, double-marginalisation is never ex-post stable. [ABSTRACT FROM AUTHOR]

The secretome of adipose-derived stromal cells (ASC) is a heterogeneous mixture of components with a beneficial influence on cellular microenvironments. As such, it represents a cell-free alternative in regenerative medicine therapies. Pathophysiological conditions increase the therapeutic capacity of ASC and, with this, the benefits of the secretome. Such conditions can be partially mimicked in vitro by adjusting culturing conditions. Secretomics, the unbiased analysis of a cell secretome by mass spectrometry, is a powerful tool to describe the composition of ASC secretomes. In this proteomics databases review, we compared ASC secretomic studies to retrieve persistently reported proteins resulting from the most explored types of culturing conditions used in research, i.e., exposure to normoxia, hypoxia, or cytokines. Our comparisons identified only eight common proteins within ASC normoxic secretomes, no commonalities within hypoxic ASC secretomes, and only nine within secretomes of ASC exposed to proinflammatory cytokines. Within these, and regardless of the culturing condition that stimulated secretion, a consistent presence of extracellular matrix-related pathways associated with such proteins was identified. Confounders such as donors' age, sex, body mass index, the anatomical area where ASC were harvested, secretome collection method, data description, and how the data is shared with the scientific community are discussed as factors that might explain our outcomes. We conclude that standardization is imperative as the currently available ASC secretomic studies do not facilitate solid conclusions on the therapeutic value of different ASC secretomes. [ABSTRACT FROM AUTHOR]

ThTi2O6 derived compounds with the brannerite structure were designed, synthesised, and characterised with the aim of stabilising incorporation of U5+ or U6+, at dilute concentration. Appropriate charge compensation was targeted by co-substitution of Gd3+, Ca2+, Al3+, or Cr3+, on the Th or Ti site. U L3 edge X-ray Absorption Near Edge Spectroscopy (XANES) and High Energy Resolution Fluorescence Detected U M4 edge XANES evidenced U5+ as the major oxidation state in all compounds, with a minor fraction of U6+ (2–13%). The balance of X-ray and Raman spectroscopy data support uranate, rather than uranyl, as the dominant U6+ speciation in the reported brannerites. It is considered that the U6+ concentration was limited by unfavourable electrostatic repulsion arising from substitution in the octahedral Th or Ti sites, which share two or three edges, respectively, with neighbouring polyhedra in the brannerite structure. [ABSTRACT FROM AUTHOR]

Unlike metals, rocks show bi-modularity (different Young’s moduli and Poisson’s ratios in compression and tension). Displacements monitored during the Brazilian test are used in this study to obtain the Young’s modulus and Poisson’s ratio in tension. New equations for the displacements in a Brazilian test are derived considering the bi-modularity in the stress–strain relations. The digital image correlation technique was used to monitor the displacements of the Brazilian disk flat surface. To validate the Young’s modulus and Poisson’s ratio obtained from the Brazilian test, the results were compared with the values from the direct tension tests. The results obtained from the Brazilian test were repetitive and within 3.5% of the value obtained from the direct tension test for the rock tested. [ABSTRACT FROM AUTHOR]

Purpose: The aims of the present study were to evaluate the development of untreated cervical intraepithelial neoplasia (CIN) 3 during pregnancy and to assess persistence, progression, and regression rates postpartum to identify factors associated with regression. Methods: In a tertiary gynecology and obstetrics department, a total of 154 pregnant women with CIN 3 were treated in the dysplasia unit. The follow-up findings were analyzed retrospectively on the basis of histological, cytological, and human papillomavirus (HPV) testing of 154 pregnant women confirmed as having CIN 3 in colposcopically guided biopsies. Results: The rates of persistence, regression, and progression of CIN 3 in these women were 76.1%, 20% and 3.2%, respectively. Data for the delivery mode was available for 126 women. The rate of regression was almost twice as high with vaginal delivery as with cesarean section, at 27.4 vs. 15.2%, whereas the rate of progression was lower with vaginal delivery, at 2.7 vs. 6.5%. Conclusion: The rate of persistence of CIN observed in this study is comparable to that reported in other studies. The study provides strong evidence for greater regression among women who have vaginal deliveries. Careful work-up is recommended postpartum for this group of women in order to rule out persistent CIN 3 or invasive disease. [ABSTRACT FROM AUTHOR]

The benefits of quantitative risk assessments for landslide management have been discussed and illustrated in several publications. However, there still are some challenges in its application for low-probability, high-magnitude events. These challenges are associated with the difficulties in populating our models for risk calculations, which largely require the input of expert opinion. This paper presents a quantitative risk assessment to a very slow moving rock slope within a dam reservoir in the Province of British Columbia, Canada. The assessment is focused on the risk to the population in the vicinity of the dam and the populated areas downstream. Expert opinions quantified the slope failure probabilities in the order of 10 to 10 per year for the smallest failure scenario considered and less than 10 for a failure of the entire slope. However, these estimations are associated with high levels of uncertainty. Our approach starts with the calculation and assessment of the magnitude and probability of the potential slope failure consequences, minimizing the uncertainties associated with estimated slope failure probabilities. Then, these consequences and failure probabilities are combined to obtain a measure of risk. The uncertainty associated with the slope failure probabilities is managed by the estimation of plausible ranges for these. The calculated risk levels are then presented as ranges of values and assessed against adopted evaluation criteria. The consequence and risk assessment of the rock slope suggest that the risk to the population exposed in the vicinity of the dam and populated areas downstream is under adequate control. The probability of large consequence scenarios is extremely low, in the order of 10 chance of an event causing more than 100 fatalities. We propose an observational technique to assess changes in risk levels and decide when to update the risk management approach or deploy emergency measures. The technique is focused on the detection of changes in the slope deformation patterns that would indicate an increase in the potential failure volumes or an imminent failure. It can be considered an extension to the current early warning system in place, easy to implement and enhanced with the strength of the comprehensive analysis required for a quantitative risk assessment. [ABSTRACT FROM AUTHOR]

The millions of specimens stored in entomological collections provide a unique opportunity to study historical insect diversity. Current technologies allow to sequence entire genomes of historical specimens and estimate past genetic diversity of present-day endangered species, advancing our understanding of anthropogenic impact on genetic diversity and enabling the implementation of conservation strategies. A limiting challenge is the extraction of historical DNA (hDNA) of adequate quality for sequencing platforms. We tested four hDNA extraction protocols on five body parts of pinned false heath fritillary butterflies, Melitaea diamina, aiming to minimise specimen damage, preserve their scientific value to the collections, and maximise DNA quality and yield for whole-genome re-sequencing. We developed a very effective approach that successfully recovers hDNA appropriate for short-read sequencing from a single leg of pinned specimens using silica-based DNA extraction columns and an extraction buffer that includes SDS, Tris, Proteinase K, EDTA, NaCl, PTB, and DTT. We observed substantial variation in the ratio of nuclear to mitochondrial DNA in extractions from different tissues, indicating that optimal tissue choice depends on project aims and anticipated downstream analyses. We found that sufficient DNA for whole genome re-sequencing can reliably be extracted from a single leg, opening the possibility to monitor changes in genetic diversity maintaining the scientific value of specimens while supporting current and future conservation strategies. [ABSTRACT FROM AUTHOR]

An instrumented section of a 10-m-diameter circular shaft located at a depth of 1.2 km in an average quality rock mass was back analyzed to establish the rock mass behavior. Extensometers were installed radially at four locations and provided the primary data for the back analyses. Three- and two-dimensional continuum models were analyzed using the numerical codes FLAC3Dand Phase2 to assess the rock mass behavior. An initial set of mechanical parameters obtained from empirical relationships were found to give a reasonable match to the measured response of extensometers 2 and 4, when using a Mohr-Coulomb strain softening model. A different set of parameters were needed for FLAC3D when trying to match the significantly higher displacements recorded by only one of the extensometer. It was noted that regardless of the material model and corresponding parameters, the three-dimensional models were not able to give reasonable match to the results of all extensometers. It was shown that for the given problem, there is a theoretical limit for ratio of displacements predicted for different extensometers using a continuum isotropic material model. The two-dimensional models in Phase2, however, gave an apparently better overall match to all the extensometers. Comparison of the results of three-dimensional models with the assumed longitudinal displacement profile for the two-dimensional model indicated that the three-dimensional effects were not adequately captured in the two-dimensional model. [ABSTRACT FROM AUTHOR]

Predicting the ground response for tunnels in weak shales remains challenging. Predicting the ground response is challenged by difficulties in characterising the material, and our ability to predict deformations that are driven by coupled hydromechanical processes, when this material yields. The techniques that are used for characterising weak shales are reviewed, and three case histories are examined that demonstrate the behaviour of these weak rocks during tunnelling. A general framework is provided for assessing the squeezing potential for weak shales. [ABSTRACT FROM AUTHOR]

Railway alignments through the Canadian Cordillera are constantly exposed to slope instabilities. Proactive mitigation strategies have been in place for a few decades now, and instability record keeping has been recognized as an important aspect of them. Such a proactive strategy has enhanced the industry's capacity to manage slope risks, and some sections have been recognized as critical due to the frequency of instabilities. At these locations, quantification of the risks becomes necessary. Risk analysis requires knowledge of some variables for which statistical data are scarce or not available, and elicitation of subjective probabilities is needed. A limitation of such approaches lies in the uncertainty associated to those elicited probabilities. In this paper, a quantitative risk analysis is presented for a section of railway across the Canadian Cordillera. The analysis focused on the risk to life of the freight train crews working along this section. Upper and lower bounds were elicited to cope with the uncertainties associated with this approach. A Monte Carlo simulation technique was then applied to obtain the probability distribution of the estimated risks. The risk probability distribution suggests that the risk to life of the crews is below previously published evaluation criteria and within acceptable levels. The risk assessment approach proposed focuses on providing a measure of the uncertainty associated with the estimated risk and is capable of handling distributions that cover more than two orders of magnitude. [ABSTRACT FROM AUTHOR]

Railways across the Canadian Cordillera have long histories of losses associated with ground hazards. The hazards most frequently reported are rockfalls, which are ubiquitous along the steep rock cuts required to accommodate the railway alignment. Several hazard control measures can be adopted in rockfall areas. However, when rockfall frequencies cannot be controlled, protective structures may be necessary to decrease rockfall-related risks to tolerable levels. Designs of protective structures require knowing rockfall trajectory heights and kinetic energies. This information is difficult to obtain even at locations where comprehensive rockfall records are kept. We present a method to calculate rockfall trajectory heights and velocities based on three-dimensional, lumped mass, rockfall simulations. Rockfall source location, model parameters and model calibration are also discussed. In this regard, the model should be calibrated against observed values of rockfall heights and velocities, and the design parameters should be validated before proceeding with the design of rockfall mitigation measures. The method is illustrated with the analysis of a section of a railway along the Canadian Cordillera. Furthermore, a probabilistic approach is adopted to calculate rockfall trajectory heights and velocities when intersecting the railway alignment. This is consistent with the natural variability of rockfall trajectories and falling block volumes. We illustrate the use of probability distributions of rockfall velocities and volumes to calculate the distribution of kinetic energy at three locations along the study section. The calculated rockfall trajectory heights are also presented in probabilistic terms and discussed. The rockfall kinetic energy distributions are used to assess the type of protective structures that could be required for further reduction of risk levels. [ABSTRACT FROM AUTHOR]

Aims: Xenin-25 is co-secreted with glucose-dependent insulinotropic polypeptide (GIP) from intestinal K-cells following a meal. Xenin-25 is believed to play a key role in glucose homoeostasis and potentiate the insulinotropic effect of GIP. Methods: This study investigated the effects of sub-chronic administration of the stable and longer-acting xenin-25 analogue, xenin-25[LysPAL] (25 nmol/kg), in diabetic mice fed with a high-fat diet. Results: Initial studies confirmed the significant persistent glucose-lowering ( p < 0.05) and insulin-releasing ( p < 0.05) actions of xenin-25[LysPAL] compared with native xenin-25. Interestingly, xenin-25 retained significant glucose-lowering activity in GIP receptor knockout mice. Twice-daily intraperitoneal (i.p.) injection of xenin-25[LysPAL] for 14 days had no significant effect on food intake or body weight in high-fat-fed mice. Non-fasting glucose and insulin levels were also unchanged, but overall glucose levels during an i.p. glucose tolerance and oral nutrient challenge were significantly ( p < 0.05) lowered by xenin-25[LysPAL] treatment. These changes were accompanied by significant improvements in i.p. ( p < 0.05) and oral ( p < 0.001) nutrient-stimulated insulin concentrations. No appreciable changes in insulin sensitivity were observed between xenin-25[LysPAL] and saline-treated high-fat mice. However, xenin-25[LysPAL] treatment restored notable sensitivity to the biological actions of exogenous GIP injection. Consumption of O, production of CO, respiratory exchange ratio and energy expenditure were not altered by 14-day twice-daily treatment with xenin-25[LysPAL]. In contrast, ambulatory activity was significantly ( p < 0.05 to p < 0.001) increased during the dark phase in xenin-25[LysPAL] mice compared with high-fat controls. Conclusions: These data indicate that sustained administration of a stable analogue of xenin-25 exerts a spectrum of beneficial metabolic effects in high-fat-fed mice. [ABSTRACT FROM AUTHOR]

A high degree of alteration causes swelling, disintegration and argillization of granite and thus weakens its physical and mechanical properties. Very few experimental studies, however, have been conducted to quantitatively characterize the strong-to-weak transition of the mechanical behaviours of granite affected by varying degrees of alteration. A new miniature tensile instrument system, which can visualize the failure process and simultaneously record the stress–strain curves, was utilized to test the tensile strength of dog-bone sliced samples of altered granite. Meanwhile, an improved quantitative method was proposed to characterize the alteration degree. The results demonstrate that the tensile strength of granite decreases significantly with increasing alteration rate, while the strain at the peak strength and fracture angle increase. Additionally, the orientation of weaker crystals and the mineral size distribution significantly affect fracture propagation. But the effect will be weakened as the alteration rate increases. On the basis of fracture angle, tensile peak strength, strain at peak strength, and microscopy images of crack initiation and propagation, three major classes of fractures are identified from the test results: (1) extension fracture at a low alteration rate: crack initiates and propagates along biotite-brittle mineral interfaces; (2) shear fracture at a high alteration rate: intragranular crack initiates within altered feldspar and propagates along the interior of altered mineral grains; (3) hybrid fracture at a moderate alteration rate: the above two failure phenomena coexist. A simple model that considers the fracture process of altered granite was proposed to explain the transition from extension fracture to shear fracture. Highlights: A new visual miniature tensile instrument is utilized to capture the crack initiation and propagation. An improved quantitative method is proposed to characterize the alteration degree of granite. As alteration degree increases, the tensile strength of granite significantly decreases, whereas both the strain and the fracture angle increase. The transition from extension fracture to shear fracture of altered granite is detected. [ABSTRACT FROM AUTHOR]

Crack initiation in uniaxial compressive loading of rocks occurs well before the peak strength is reached. The factors that may influence the onset of cracking and possible initiating mechanisms were explored using a discrete element numerical approach. The numerical approach was based on grain-based model that utilized the Voronoi tessellation scheme to represent low porosity crystalline rocks such as granite. The effect of grain size distribution (sorting coefficient ranging from 1.5 to 1.03), grain size (average grain size ranging from 0.75 to 2.25 mm), and the heterogeneities of different mineral grains (quartz, K-feldspar, plagioclase) on the onset of cracking were examined. The modelling revealed that crack initiation appears to be a tensile mechanism in low porosity rocks, and that shear cracking along grain boundaries is only a prominent mechanism near the peak strength. It was also shown that the heterogeneity introduced by the grain size distribution had the most significant effect on peak strength and crack initiation stress. The peak strength ranges from 140 to 208 MPa as the grain size distribution varies from heterogeneous to uniform, respectively. However, the ratio of crack initiation to peak stress showed only minor variation, as the heterogeneity decreases. The other factors investigated had only minor effects on crack initiation and peak strength, and crack initiation ratio. [ABSTRACT FROM AUTHOR]

Bioconstructions such as coralligenous outcrops and maërl beds are typical Mediterranean underwater seascapes. Fine-scale knowledge on the distribution of these sensitive habitats is crucial for their effective management and conservation. In the present study, a thorough review of existing spatial datasets showing the distribution of coralligenous and maërl habitats across the Mediterranean Sea was undertaken, highlighting current gaps in knowledge. Predictive modelling was then carried out, based on environmental predictors, to produce the first continuous maps of these two habitats across the entire basin. These predicted occurrence maps for coralligenous outcrops and maërl beds provide critical information about where the two habitats are most likely to occur. The collated occurrence data and derived distribution model outputs can help addressing the challenge of developing basin-wide spatial plans and to guide cost-effective future surveys and monitoring efforts towards areas that are presently poorly-sampled. [ABSTRACT FROM AUTHOR]

Multilocus sequence typing (MLST) was proposed in 1998 as a portable sequence-based method for identifying clonal relationships among bacteria. Today, in the whole-genome era of microbiology, the need for systematic, standardized descriptions of bacterial genotypic variation remains a priority. Here, to meet this need, we draw on the successes of MLST and 16S rRNA gene sequencing to propose a hierarchical gene-by-gene approach that reflects functional and evolutionary relationships and catalogues bacteria 'from domain to strain'. Our gene-based typing approach using online platforms such as the Bacterial Isolate Genome Sequence Database (BIGSdb) allows the scalable organization and analysis of whole-genome sequence data. [ABSTRACT FROM AUTHOR]

Background: Lung volume optimization maneuvers (LVOM) are necessary to make physiologic use of high-frequency oscillatory ventilation (HFOV), but lung behavior during such maneuvers has not been studied to determine lung volume changes after initiation of HFOV, to quantify recruitment versus derecruitment during the LVOM and to calculate the time to stabilization after a pressure change. Methods: We performed a secondary analysis of prospectively collected data in subjects < 18 years on HFOV. Uncalibrated respiratory inductance plethysmography (RIP) tracings were used to quantify lung recruitment and derecruitment during the LVOM inflation and deflation. The time constant was calculated according to the Niemann model. Results: RIP data of 51 subjects (median age 3.5 [1.7–13.3] months) with moderate-to-severe pediatric acute respiratory distress syndrome (PARDS) in 85.4% were analyzed. Lung recruitment and derecruitment occurred during the LVOM inflation phase upon start of HFOV and between and within pressure changes. At 90% of maximum inflation pressure, lung derecruitment already started during the deflation phase. Time to stable lung volume (time constant) could only be calculated in 26.2% of all pressure changes during the inflation and in 21.4% during the deflation phase, independent of continuous distending pressure (CDP). Inability to calculate the time constant was due to lack of stabilization of the RIP signal or no change in any direction. Conclusions: Significant heterogeneity in lung behavior during a staircase incremental–decremental LVOM occurred, underscoring the need for higher initial inflation pressures when transitioning from conventional mechanical ventilation (CMV) and a longer time between pressure changes to allow for equilibration. [ABSTRACT FROM AUTHOR]

Coal mining is known for its contributions to climate change, but its impacts on the environment and human lives near mine sites are less widely recognised. This study integrates remote sensing, GIS, stakeholder interviews and extensive review of provincial data and documents to identify patterns of infringement, risk and impact driven by coal mining expansion across East Kalimantan, Indonesia. Specifically, we map and analyse patterns of mining concessions, land clearing, water cover, human settlement, and safety risks, and link them with mining governance and regulatory infractions related to coal mining permits. We show that excessive, improper permit granting and insufficient monitoring and oversight have led to deforestation, widespread overlaps of concessions with settlements, extensive boundary and regulatory violations, lacking reclamation, and numerous deaths. As the world's largest thermal coal exporter, Indonesia's elevated coal infringements, risks, and impacts translate to supply chain, sustainability, and human rights concerns for global coal markets. [ABSTRACT FROM AUTHOR]