16 results on '"Guo-Ping Shi"'
Search Results
2. Differential IL18 signaling via IL18 receptor and Na-Cl co-transporter discriminating thermogenesis and glucose metabolism regulation
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Xian Zhang, Songyuan Luo, Minjie Wang, Qiongqiong Cao, Zhixin Zhang, Qin Huang, Jie Li, Zhiyong Deng, Tianxiao Liu, Cong-Lin Liu, Mathilde Meppen, Amelie Vromman, Richard A. Flavell, Gökhan S. Hotamışlıgil, Jian Liu, Peter Libby, Zhangsuo Liu, and Guo-Ping Shi
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Mice ,Receptors, Interleukin-18 ,Glucose ,Multidisciplinary ,Symporters ,Interleukin-18 ,Animals ,General Physics and Astronomy ,General Chemistry ,Insulin Resistance ,General Biochemistry, Genetics and Molecular Biology - Abstract
White adipose tissue (WAT) plays a role in storing energy, while brown adipose tissue (BAT) is instrumental in the re-distribution of stored energy when dietary sources are unavailable. Interleukin-18 (IL18) is a cytokine playing a role in T-cell polarization, but also for regulating energy homeostasis via the dimeric IL18 receptor (IL18r) and Na-Cl co-transporter (NCC) on adipocytes. Here we show that IL18 signaling in metabolism is regulated at the level of receptor utilization, with preferential role for NCC in brown adipose tissue (BAT) and dominantly via IL18r in WAT. In Il18r−/−Ncc−/− mice, high-fat diet (HFD) causes more prominent body weight gain and insulin resistance than in wild-type mice. The WAT insulin resistance phenotype of the double-knockout mice is recapitulated in HFD-fed Il18r−/− mice, whereas decreased thermogenesis in BAT upon HFD is dependent on NCC deletion. BAT-selective depletion of either NCC or IL18 reduces thermogenesis and increases BAT and WAT inflammation. IL18r deletion in WAT reduces insulin signaling and increases WAT inflammation. In summary, our study contributes to the mechanistic understanding of IL18 regulation of energy metabolism and shows clearly discernible roles for its two receptors in brown and white adipose tissues.
- Published
- 2022
3. Depressive symptoms are associated with incident frailty in a Chinese population: the Rugao Longevity and Aging Study
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Shun Yao, Jiang-Hong Guo, Jianming Shi, Yu-Chen Wang, Xiaofeng Wang, Guo-Ping Shi, Xuefeng Chu, Yinsheng Zhu, Xiaoyan Jiang, Zhengdong Wang, Na Zhang, and Yong Wang
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Gerontology ,Aging ,Frail Elderly ,media_common.quotation_subject ,Longevity ,Older population ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,Humans ,Medicine ,030212 general & internal medicine ,Risk factor ,Geriatric Assessment ,Depression (differential diagnoses) ,Depressive symptoms ,Aged ,media_common ,Chinese population ,Frailty ,Depression ,business.industry ,Chinese adults ,Middle Aged ,Cross-Sectional Studies ,Geriatric Depression Scale ,Geriatrics and Gerontology ,business ,030217 neurology & neurosurgery - Abstract
This study aimed at investigating whether depression symptoms are associated with prevalent and incident physical frailty in Chinese older population. We analyzed data of 1168 older Chinese adults aged 70 and above in the aging arm of the Rugao Longevity and Aging Study (RuLAS). Depressive symptoms (Geriatric Depression Scale ≥ 6) were assessed by the Geriatric Depression Scale. Frailty was defined using Fried phenotype criteria at baseline and 3-year survey. At baseline, 8.9% of the participants had depression symptoms. The prevalence of pre-frailty and frailty were 34.5% and 5.9%, respectively. The percentages of depressive symptoms increase from robust (5.3%) to pre-frail (11.2%), and then to frail (31.9%) groups. After adjustments of multiple covariates, depressive symptoms were associated with both prevalent pre-frailty (OR = 1.75, 95% CI 1.08–2.84) and prevalent frailty (OR = 5.64, 95% CI 2.85–11.14) at baseline. At 3-year survey, 9.3% participants reported the development of frailty. After multiple adjustments, depressive symptoms were associated with a 2.79-fold (95% CI 1.09–7.10) increased risk of 3-year incident frailty. Depressive symptoms are associated with prevalent and incident frailty in Chinese older population. Together with the observations of the European populations, depressive symptoms may be a candidate risk factor of frailty.
- Published
- 2019
4. Losartan accelerates the repair process of renal fibrosis in UUO mouse after the surgical recanalization by upregulating the expression of Tregs
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Wei Zhu, Chunming Jiang, Xiang Yan, Guo-Ping Shi, Yangyang Xia, Miao Zhang, Jia Luo, and Jie Song
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Male ,Nephrology ,medicine.medical_specialty ,Time Factors ,Urology ,030232 urology & nephrology ,Renal function ,Angiotensin-Converting Enzyme Inhibitors ,030204 cardiovascular system & hematology ,Kidney ,urologic and male genital diseases ,T-Lymphocytes, Regulatory ,Losartan ,Mice ,Random Allocation ,03 medical and health sciences ,0302 clinical medicine ,Fibrosis ,Internal medicine ,medicine ,Renal fibrosis ,Animals ,business.industry ,medicine.disease ,Obstructive Nephropathy ,Up-Regulation ,Mice, Inbred C57BL ,Treatment Outcome ,medicine.anatomical_structure ,business ,Ureteral Obstruction ,Kidney disease ,medicine.drug - Abstract
Obstructive nephropathy is a common cause for chronic kidney disease. Surgery, which is adopted to promptly relieve the obstruction, is the most important method to save damaged kidneys. However, earlier studies have shown that renal function will continue to deteriorate until the terminal stage after the obstruction' relief. The aim of this study is to explore the renal fibrosis and investigate the effect of losartan on renal fibrosis after the obstruction' relief using an improved mouse model of relief for unilateral ureteral obstruction (RUUO). Experiments carried out using C57BL/6 mice (n = 30) were randomly divided into RUUO + Losartan group, RUUO group and sham group. Using an improved mouse RUUO model, this study revealed that the mouse kidney for 3- or 7-day unilateral ureteral obstruction undergoing the RUUO surgery was still in a state of injury and fibrosis, while losartan could effectively ameliorate renal fibrosis by upregulating the expression of CD4 + CD25 + Foxp3 + regulatory T cells (Tregs) in kidney after the surgery of RUUO.
- Published
- 2019
5. Cathepsin K Knockout Exacerbates Haemorrhagic Transformation Induced by Recombinant Tissue Plasminogen Activator After Focal Cerebral Ischaemia in Mice
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Bing-Qiao Zhao, Yue Hu, Rong Zhao, Xiao-Yan Feng, Yi-Sheng Liu, Lei Zhao, Xin-Wei He, Yan-Hui Shi, Hui-Qin Liu, Mei-Ting Zhuang, Guo-Ping Shi, Jian-Ren Liu, and Feng-Di Liu
- Subjects
Male ,Vascular Endothelial Growth Factor A ,0301 basic medicine ,medicine.medical_specialty ,Cathepsin K ,Apoptosis ,Permeability ,Brain Ischemia ,Neovascularization ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Macrophage ,Protein kinase B ,PI3K/AKT/mTOR pathway ,Cerebral Hemorrhage ,Mice, Knockout ,Neurons ,Microglia ,business.industry ,Macrophages ,TOR Serine-Threonine Kinases ,Infarction, Middle Cerebral Artery ,Cell Biology ,General Medicine ,Recombinant Proteins ,Vascular endothelial growth factor ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,nervous system ,chemistry ,Blood-Brain Barrier ,Tissue Plasminogen Activator ,Knockout mouse ,medicine.symptom ,business ,Proto-Oncogene Proteins c-akt ,030217 neurology & neurosurgery ,Signal Transduction - Abstract
Severe haemorrhagic transformation (HT), a common complication of recombinant tissue plasminogen activator (rtPA) treatment, predicts poor clinical outcomes in acute ischaemic stroke. The search for agents to mitigate this effect includes investigating biomolecules involved in neovascularization. This study examines the role of Cathepsin K (Ctsk) in rtPA-induced HT after focal cerebral ischaemia in mice. After knockout of Ctsk, the gene encoding Ctsk, the outcomes of Ctsk+/+ and Ctsk−/− mice were compared 24 h after rtPA-treated cerebral ischaemia with respect to HT severity, neurological deficits, brain oedema, infarct volume, number of apoptotic neurons and activated microglia/macrophage, blood–brain barrier integrity, vascular endothelial growth factor (VEGF) expression and Akt-mTOR pathway activation. We observed that haemoglobin levels, brain oedema and infarct volume were significantly greater and resulted in more severe neurological deficits in Ctsk−/− than in Ctsk+/+ mice. Consistent with our hypothesis, the number of NeuN-positive neurons was lower and the number of TUNEL-positive apoptotic neurons and activated microglia/macrophage was higher in Ctsk−/− than in Ctsk+/+ mice. Ctsk knockout mice exhibited more severe blood–brain barrier (BBB) disruption, with microvascular endothelial cells exhibiting greater VEGF expression and lower ratios of phospo-Akt/Akt and phospo-mTOR/mTOR than in Ctsk+/+ mice. This study is the first to provide molecular insights into Ctsk-regulated HT after cerebral ischaemia, suggesting that Ctsk deficiency may disrupt the BBB via Akt/mTOR/VEGF signalling, resulting in neurological deficits and neuron apoptosis. Ctsk administration has the potential as a novel modality for improving the safety of rtPA treatment following stroke.
- Published
- 2019
6. Association of BNP with Frailty in Elderly Population: Rugao Longevity and Ageing Study
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Xuefeng Chu, Jiang-Hong Guo, Li Jin, Yong Wang, Zhengdong Wang, Xiaoyan Jiang, Shun Yao, Xiaofeng Wang, Yinsheng Zhu, and Guo-Ping Shi
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Male ,Aging ,Weakness ,medicine.medical_specialty ,030309 nutrition & dietetics ,medicine.drug_class ,Frail Elderly ,media_common.quotation_subject ,Longevity ,Medicine (miscellaneous) ,Cohort Studies ,03 medical and health sciences ,Grip strength ,0302 clinical medicine ,Weight loss ,Internal medicine ,Natriuretic Peptide, Brain ,medicine ,Natriuretic peptide ,Humans ,Prospective Studies ,030212 general & internal medicine ,Prospective cohort study ,Aged ,media_common ,Aged, 80 and over ,0303 health sciences ,Nutrition and Dietetics ,business.industry ,Physical activity level ,Cross-Sectional Studies ,Ageing ,Female ,Geriatrics and Gerontology ,medicine.symptom ,business ,human activities - Abstract
To explore the associations of B-type natriuretic peptide (BNP) with physical frailty status as well as each domain of frailty in a general elderly population. Cross-sectional analysis of prospective cohort study. All of 31 communities in Jiang’an township. Overall 1338 participants (aged 70–89 years, mean 77.42±4.08 years) without a history of cardiovascular diseases in the third-wave of the aging arm of the Rugao Longevity and Aging Study (RuLAS). Frailty was defined as the presence of ≥3 domains among five modified Fried’s criteria (unintentional weight loss, low physical activity level, weakness (low grip strength), exhaustion, and slowness (slow gait speed)) and pre-frailty as the presence of 1–2 domains. The prevalence of frailty and pre-frailty was 10.4% and 53.3%, respectively, in this elderly population. Elevated BNP (≥100 pg/mL) was significantly associated with pre-frailty (OR: 1.61, 95% CI: 1.13-2.29) and frailty (OR: 2.63, 95% CI: 1.61-4.32) after adjustment for covariates. In addition, elevated BNP was associated with low grip strength (OR: 2.00, 95% CI: 1.41-2.82) and low gait speed (OR: 1.62, 95% CI: 1.15-2.28) after adjustment for multiple covariates. Log BNP was inversely associated with grip strength (r= -0.265, p
- Published
- 2018
7. Na+-H+ exchanger 1 determines atherosclerotic lesion acidification and promotes atherogenesis
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Jin Ying Zhang, Samuel Achilefu, Xian Zhang, Cong-Lin Liu, Tianxiao Liu, Rui Tang, Peter Libby, Yunzhe Wang, Jing Liu, Galina K. Sukhova, Guo-Ping Shi, Gregory R. Wojtkiewicz, Junli Guo, and Matthias Nahrendorf
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0301 basic medicine ,Science ,General Physics and Astronomy ,02 engineering and technology ,Immunoglobulin E ,General Biochemistry, Genetics and Molecular Biology ,Lesion ,03 medical and health sciences ,medicine ,Extracellular ,Macrophage ,lcsh:Science ,Receptor ,Foam cell ,Multidisciplinary ,biology ,Chemistry ,Colocalization ,General Chemistry ,021001 nanoscience & nanotechnology ,Molecular biology ,3. Good health ,030104 developmental biology ,Apoptosis ,biology.protein ,lcsh:Q ,medicine.symptom ,0210 nano-technology - Abstract
The pH in atherosclerotic lesions varies between individuals. IgE activates macrophage Na+-H+ exchanger (Nhe1) and induces extracellular acidification and cell apoptosis. Here, we show that the pH-sensitive pHrodo probe localizes the acidic regions in atherosclerotic lesions to macrophages, IgE, and cell apoptosis. In Apoe–/– mice, Nhe1-deficiency or anti-IgE antibody reduces atherosclerosis and blocks lesion acidification. Reduced atherosclerosis in Apoe–/– mice receiving bone marrow from Nhe1- or IgE receptor FceR1-deficient mice, blunted foam cell formation and signaling in IgE-activated macrophages from Nhe1-deficient mice, immunocomplex formation of Nhe1 and FceR1 in IgE-activated macrophages, and Nhe1-FceR1 colocalization in atherosclerotic lesion macrophages support a role of IgE-mediated macrophage Nhe1 activation in atherosclerosis. Intravenous administration of a near-infrared fluorescent pH-sensitive probe LS662, followed by coregistered fluorescent molecular tomography-computed tomography imaging, identifies acidic regions in atherosclerotic lesions in live mice, ushering a non-invasive and radiation-free imaging approach to monitor atherosclerotic lesions in live subjects. Na+-H+ exchanger 1 (Nhe1) regulates extracellular pH by extruding protons in exchange for extracellular Na+ . Here, Liu et al. show that Nhe1 promotes the development and acidification of atherosclerotic lesions and that pH-sensitive probes can be used to monitor plaque growth and acidification.
- Published
- 2019
8. Mast cells in human and experimental cardiometabolic diseases
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Petri T. Kovanen, Guo-Ping Shi, and Ilze Bot
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Chemokine ,Proteases ,Adipose tissue ,Inflammation ,Article ,Extracellular matrix ,Pathogenesis ,chemistry.chemical_compound ,Metabolic Diseases ,medicine ,Animals ,Humans ,Mast Cells ,biology ,business.industry ,Heparin ,Disease Models, Animal ,chemistry ,Cardiovascular Diseases ,Immunology ,biology.protein ,Inflammation Mediators ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Histamine ,medicine.drug - Abstract
Mast cells, like many other types of inflammatory cell, perform pleiotropic roles in cardiometabolic diseases such as atherosclerosis, abdominal aortic aneurysms, obesity, and diabetes mellitus, as well as complications associated with these diseases. Low numbers of mast cells are present in the heart, aorta, and adipose tissue of healthy humans, but patients with cardiometabolic diseases and animals with experimentally-induced cardiometabolic pathologies have high numbers of mast cells with increased activity in the affected tissues. Mediators released by the activated mast cells, such as chemokines, cytokines, growth factors, heparin, histamine, and proteases, not only function as biomarkers of cardiometabolic diseases, but might also directly contribute to the pathogenesis of such diseases. Mast-cell mediators impede the functions of vascular cells, the integrity of the extracellular matrix, and the activity of other inflammatory cells, thereby contributing to the pathobiology of the conditions at multiple levels. In mouse models, mast-cell activation aggravates the progression of various cardiometabolic pathologies, whereas a genetic deficiency or pharmacological stabilization of mast cells, or depletion or inhibition of specific mast-cell mediators, tends to delay the progression of such conditions. Pharmacological inhibition of mast-cell activation or their targeted effector functions offers potential novel therapeutic strategies for patients with cardiometabolic disorders.
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- 2015
9. Whole blood microRNA markers are associated with acute respiratory distress syndrome
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Liming Liang, Zhaoxi Wang, David C. Christiani, Zhaozhong Zhu, Yongyue Wei, Yichen Guo, Guo-Ping Shi, Paula Tejera, Li Su, Ruyang Zhang, Andrea A. Baccarelli, Quan Lu, Xi Zhu, B. Taylor Thompson, and Ednan K. Bajwa
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0301 basic medicine ,Oncology ,Pathology ,medicine.medical_specialty ,ARDS ,Lung injury ,Critical Care and Intensive Care Medicine ,Logistic regression ,Sepsis ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Whole blood ,Receiver operating characteristic ,business.industry ,Research ,lcsh:Medical emergencies. Critical care. Intensive care. First aid ,MicroRNA ,lcsh:RC86-88.9 ,medicine.disease ,3. Good health ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cohort ,Biomarker (medicine) ,LIPS ,business - Abstract
Background MicroRNAs (miRNAs) can play important roles in inflammation and infection, which are common manifestations of acute respiratory distress syndrome (ARDS). We assessed if whole blood miRNAs were potential diagnostic biomarkers for human ARDS. Methods This nested case-control study (N = 530) examined a cohort of ARDS patients and critically ill at-risk controls. Whole blood miRNA profiles and logistic regression analyses identified miRNAs correlated with ARDS. Stratification analysis also assessed selected miRNA markers for their role in sepsis and pneumonia associated with ARDS. Receiver operating characteristic (ROC) analysis evaluated miRNA diagnostic performance, along with Lung Injury Prediction Score (LIPS). Results Statistical analyses were performed on 294 miRNAs, selected from 754 miRNAs after quality control screening. Logistic regression identified 22 miRNAs from a 156-patient discovery cohort as potential risk or protective markers of ARDS. Three miRNAs—miR-181a, miR-92a, and miR-424—from the discovery cohort remained significantly associated with ARDS in a 373-patient independent validation cohort (FDR q
- Published
- 2017
10. IL-17A promotes ventricular remodeling after myocardial infarction
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Jing Yuan, Wen-cai Zhang, Xin Tu, Qing Wang, Zheng Feng Zhu, Ni Xia, Meng Yang Liao, Jing Jing Li, Shao Fang Nie, Hong Xiao, Jiao Jiao, Su Feng Zhou, Wen Yong Dong, Yuhua Liao, Tingting Tang, Guo-Ping Shi, Xiang Cheng, and Bing Jie Lv
- Subjects
Male ,MAPK/ERK pathway ,medicine.medical_specialty ,p38 mitogen-activated protein kinases ,Myocardial Infarction ,Apoptosis ,p38 Mitogen-Activated Protein Kinases ,Internal medicine ,Drug Discovery ,medicine ,Animals ,Myocytes, Cardiac ,RNA, Messenger ,Myocardial infarction ,Ventricular remodeling ,Genetics (clinical) ,bcl-2-Associated X Protein ,Receptors, Interleukin-17 ,Ventricular Remodeling ,business.industry ,Myocardium ,Interleukin-17 ,Cytochromes c ,medicine.disease ,Fibrosis ,Mice, Inbred C57BL ,Heart failure ,Cardiology ,Cancer research ,Molecular Medicine ,Myocardial fibrosis ,Tumor Suppressor Protein p53 ,Signal transduction ,business ,Reperfusion injury - Abstract
Inflammatory responses play an important role in the pathogenesis of adverse ventricular remodeling after myocardial infarction (MI). We previously demonstrated that interleukin (IL)-17A plays a pathogenic role in myocardial ischemia/reperfusion injury and viral myocarditis. However, the role of IL-17A in post-MI remodeling and the related mechanisms have not been fully elucidated. Acute MI was induced by permanent ligation of the left anterior descending coronary artery in C57BL/6 mice. Repletion of IL-17A significantly aggravated both early- and late-phase ventricular remodeling, as demonstrated by increased infarct size, deteriorated cardiac function, increased myocardial fibrosis, and cardiomyocyte apoptosis. By contrast, genetic IL-17A deficiency had the opposite effect. Additional studies in vitro indicated that IL-17A induces neonatal cardiomyocyte (from C57BL/6 mice) apoptosis through the activation of p38, p53 phosphorylation, and Bax redistribution. These data demonstrate that IL-17A induces cardiomyocyte apoptosis through the p38 mitogen-activated protein kinase (MAPK)-p53-Bax signaling pathway and promotes both early- and late-phase post-MI ventricular remodeling. IL-17A might be an important target in preventing heart failure after MI. Key message: We demonstrated that IL-17A plays a pathogenic role both in the early and late stages of post-MI remodeling. IL-17A induces murine cardiomyocyte apoptosis. IL-17A induces murine cardiomyocyte apoptosis through the p38 MAPK-p53-Bax signaling pathway.
- Published
- 2014
11. Cathepsin S inhibition lowers blood glucose levels in mice
- Author
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Karine Clément, Jean-Charles Lafarge, Véronique Pelloux, Guo-Ping Shi, Thierry Sulpice, Michèle Guerre-Millo, Nicolas Venteclef, Guido Hartmann, Maria Pini, and Gabriela Orasanu
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Blood Glucose ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Respiratory chain ,Adipose tissue ,Type 2 diabetes ,Biology ,Diet, High-Fat ,Mice ,Oxygen Consumption ,Downregulation and upregulation ,Internal medicine ,Internal Medicine ,medicine ,Animals ,Insulin ,Glucose homeostasis ,Obesity ,Cathepsin S ,Mice, Knockout ,CATS ,medicine.disease ,Cathepsins ,Endocrinology ,Knockout mouse ,Insulin Resistance - Abstract
Cathepsin S (CatS) belongs to a family of proteases that have been implicated in several disease processes. We previously identified CatS as a protein that is markedly overexpressed in adipose tissue of obese individuals and downregulated after weight loss and amelioration of glycaemic status induced by gastric bypass surgery. This prompted us to test whether the protease contributes to the pathogenesis of type 2 diabetes using mouse models with CatS inactivation. CatS knockout mice and wild-type mice treated with orally active small-molecule CatS inhibitors were fed chow or high-fat diets and explored for change in glycaemic status. CatS deletion induced a robust reduction in blood glucose, which was preserved in diet-induced obesity and with ageing and was recapitulated with CatS inhibition in obese mice. In vivo testing of glucose tolerance, insulin sensitivity and glycaemic response to gluconeogenic substrates revealed that CatS suppression reduced hepatic glucose production despite there being no improvement in insulin sensitivity. This phenotype relied on downregulation of gluconeogenic gene expression in liver and a lower rate of hepatocellular respiration. Mechanistically, we found that the protein ‘regulated in development and DNA damage response 1’ (REDD1), a factor potentially implicated in reduction of respiratory chain activity, was overexpressed in the liver of mice with CatS deficiency. Our results revealed an unexpected metabolic effect of CatS in promoting pro-diabetic alterations in the liver. CatS inhibitors currently proposed for treatment of autoimmune diseases could help to lower hepatic glucose output in obese individuals at risk for type 2 diabetes.
- Published
- 2014
12. Endothelial cell-fatty acid binding protein 4 promotes angiogenesis: role of stem cell factor/c-kit pathway
- Author
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Samantha Traphagen, Haiming Cao, Sule Cataltepe, Manuela Cernadas, Jorge Plutzky, Guo-Ping Shi, Harun Elmasri, Chen-Wei Yu, Gökhan S. Hotamisligil, Mustafa Sahin, and Elisa Ghelfi
- Subjects
Cancer Research ,Cell type ,Endothelium ,Cell Survival ,Physiology ,Angiogenesis ,Blotting, Western ,Clinical Biochemistry ,Neovascularization, Physiologic ,Apoptosis ,Stem cell factor ,mTORC1 ,Biology ,Fatty Acid-Binding Proteins ,Article ,Mice ,Downregulation and upregulation ,medicine ,Animals ,Humans ,Cells, Cultured ,Mice, Knockout ,Stem Cell Factor ,Chemotaxis ,Endothelial Cells ,Cell biology ,Endothelial stem cell ,medicine.anatomical_structure ,Gene Expression Regulation ,RNA Interference ,Endothelium, Vascular ,Signal transduction - Abstract
Fatty acid binding protein 4 (FABP4) plays an important role in regulation of glucose and lipid homeostasis as well as inflammation through its actions in adipocytes and macrophages. FABP4 is also expressed in a subset of endothelial cells, but its role in this cell type is not known. We found that FABP4-deficient human umbilical vein endothelial cells (HUVECs) demonstrate a markedly increased susceptibility to apoptosis as well as decreased migration and capillary network formation. Aortic rings from FABP4(-/-) mice demonstrated decreased angiogenic sprouting, which was recovered by reconstitution of FABP4. FABP4 was strongly regulated by mTORC1 and inhibited by Rapamycin. FABP4 modulated activation of several important signaling pathways in HUVECs, including downregulation of P38, eNOS, and stem cell factor (SCF)/c-kit signaling. Of these, the SCF/c-kit pathway was found to have a major role in attenuated angiogenic activity of FABP4-deficient ECs as provision of exogenous SCF resulted in a significant recovery in cell proliferation, survival, morphogenesis, and aortic ring sprouting. These data unravel a novel pro-angiogenic role for endothelial cell-FABP4 and suggest that it could be exploited as a potential target for diseases associated with pathological angiogenesis.
- Published
- 2012
13. Cathepsin L activity controls adipogenesis and glucose tolerance
- Author
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Min Yang, Yaou Zhang, Galina K. Sukhova, Alessandro Doria, Jiusong Sun, Jian Liu, Peter Libby, Barbara B. Kahn, Michèle Guerre-Millo, Guo-Ping Shi, Jie-Hong Pan, Odile D. Peroni, Nobuhiko Katunuma, and Karine Clément
- Subjects
Male ,medicine.medical_specialty ,Pyridines ,Cathepsin L ,medicine.medical_treatment ,Glucose uptake ,Mice, Obese ,Carbohydrate metabolism ,Receptor, IGF Type 1 ,Mice ,Internal medicine ,Glucose Intolerance ,Adipocytes ,CCAAT-Enhancer-Binding Protein-alpha ,medicine ,Animals ,Humans ,Receptor ,Cells, Cultured ,Mice, Knockout ,Adipogenesis ,biology ,Insulin ,Body Weight ,Glucose transporter ,Cell Differentiation ,Cell Biology ,Cathepsins ,Receptor, Insulin ,Fibronectins ,Mice, Inbred C57BL ,PPAR gamma ,Cysteine Endopeptidases ,Insulin receptor ,Glucose ,Endocrinology ,biology.protein ,Epoxy Compounds - Abstract
Cysteine proteases play an important part in human pathobiology. This report shows the participation of cathepsin L (CatL) in adipogenesis and glucose intolerance. In vitro studies demonstrate the role of CatL in the degradation of the matrix protein fibronectin, insulin receptor (IR) and insulin-like growth factor-1 receptor (IGF-1R), essential molecules for adipogenesis and glucose metabolism. CatL inhibition leads to the reduction of human and murine pre-adipocyte adipogenesis or lipid accumulation, protection of fibronectin from degradation, accumulation of IR and IGF-1R beta-subunits, and an increase in glucose uptake. CatL-deficient mice are lean and have reduced levels of serum glucose and insulin but increased levels of muscle IR beta-subunits, fibronectin and glucose transporter (Glut)-4, although food/water intake and energy expenditure of these mice are no less than their wild-type littermates. Importantly, the pharmacological inhibition of CatL also demonstrates reduced body weight gain and serum insulin levels, and increased glucose tolerance, probably due to increased levels of muscle IR beta-subunits, fibronectin and Glut-4 in both diet-induced obese mice and ob/ob mice. Increased levels of CatL in obese and diabetic patients suggest that this protease is a novel target for these metabolic disorders.
- Published
- 2007
14. Mast cells promote atherosclerosis by releasing proinflammatory cytokines
- Author
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Guo-Ping Shi, Paul J. Wolters, Shiro Kitamoto, Min Yang, Peter Libby, Jon Mallen-St. Clair, Jiusong Sun, Galina K. Sukhova, and Lindsey A MacFarlane
- Subjects
Male ,Adoptive cell transfer ,Mice, Transgenic ,General Biochemistry, Genetics and Molecular Biology ,Proinflammatory cytokine ,Mice ,Animals ,Humans ,Medicine ,Macrophage ,Mast Cells ,Interleukin 5 ,Mice, Knockout ,Innate immune system ,business.industry ,General Medicine ,Atherosclerosis ,Mast cell ,Mice, Inbred C57BL ,Interleukin 33 ,medicine.anatomical_structure ,Immunology ,Cancer research ,Cytokines ,Tumor necrosis factor alpha ,Inflammation Mediators ,business - Abstract
Mast cells contribute importantly to allergic and innate immune responses by releasing various preformed and newly synthesized mediators. Previous studies have shown mast cell accumulation in human atherosclerotic lesions. This report establishes the direct participation of mast cells in atherogenesis in low-density lipoprotein receptor-deficient (Ldlr(-/-)) mice. Atheromata from compound mutant Ldlr(-/-) Kit(W-sh)(/W-sh) mice showed decreased lesion size, lipid deposition, T-cell and macrophage numbers, cell proliferation and apoptosis, but increased collagen content and fibrous cap development. In vivo, adoptive transfer of syngeneic wild-type or tumor necrosis factor (TNF)-alpha-deficient mast cells restored atherogenesis to Ldlr(-/-)Kit(W-sh/W-sh) mice. Notably, neither interleukin (IL)-6- nor interferon (IFN)-gamma-deficient mast cells did so, indicating that the inhibition of atherogenesis in Ldlr(-/-)Kit(W-sh/W-sh) mice resulted from the absence of mast cells and mast cell-derived IL-6 and IFN-gamma. Compared with wild-type or TNF-alpha-deficient mast cells, those lacking IL-6 or IFN-gamma did not induce expression of proatherogenic cysteine proteinase cathepsins from vascular cells in vitro or affect cathepsin and matrix metalloproteinase activities in atherosclerotic lesions, implying that mast cell-derived IL-6 and IFN-gamma promote atherogenesis by augmenting the expression of matrix-degrading proteases. These observations establish direct participation of mast cells and mast cell-derived IL-6 and IFN-gamma in mouse atherogenesis and provide new mechanistic insight into the pathogenesis of this common disease.
- Published
- 2007
15. Image-guided percutaneous renal cryoablation for stage 1 renal cell carcinoma with high surgical risk
- Author
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Weidong Gan, Rong Yang, Xiaoxiang Chen, Xiang Yan, Wang Wei, Mingxin Zhang, Guo-Ping Shi, Hongqian Guo, Yang Yang, and Yang Wang
- Subjects
Cryoablation ,Local anesthesia ,Adult ,Male ,medicine.medical_specialty ,Percutaneous ,medicine.medical_treatment ,Cryosurgery ,Surgical oncology ,Renal cell carcinoma ,medicine ,Humans ,Stage (cooking) ,Carcinoma, Renal Cell ,Aged ,Neoplasm Staging ,Aged, 80 and over ,medicine.diagnostic_test ,business.industry ,Research ,Endoscopy ,Middle Aged ,medicine.disease ,Kidney Neoplasms ,Surgery, Computer-Assisted ,Oncology ,Female ,Surgery ,Radiology ,business - Abstract
Background This study was undertaken to evaluate the feasibility, safety, and therapeutic effects of percutaneous renal cryoablation under local anesthesia with conscious sedation for patients who have unresectable stage 1 (T1NoMo) renal cell carcinoma (RCC) in high surgical risk. Methods Eighteen patients who were not candidates for surgery underwent primary cryosurgery guided by gray-scale ultrasound. Contrast-enhanced ultrasonography (CEUS) and contrast-enhanced computed tomography (CT) were performed to evaluate treatment at completion. Results The mean follow-up period was 26.8 months (range, 12–56 months). All tumors were biopsied before cryosurgery. Seventeen tumors remained free of enhancement during follow-up period. No major complications associated with cryoablation procedures were found though two instances of subcapsular hematomas, one of retroperitoneal errhysis and one of nausea, were seen after cryoablation. One patient had a local recurrence of tumor and received additional cryoablation. Local tumor control rate was 100 % of T1NoMo tumors including the recurrence case who underwent additional cryoablation. Conclusions Percutaneous cryoablation can be recommended as a feasible, safe, and promising therapy for the treatment of renal tumor, especially those unresectable stage 1 RCC, with a low risk of complications.
- Published
- 2015
16. Erratum: Enhanced hypertension prevalence in non-Han Chinese minorities from Xinjiang Province, China
- Author
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Zhensui Lu, Zhenhong Lu, Yanling Zhu, Zhaoyang Yan, Xiaoquan Liu, Shigui Yan, and Guo-Ping Shi
- Subjects
Physiology ,Internal Medicine ,Cardiology and Cardiovascular Medicine - Published
- 2010
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