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The development of a sensitive biochemical assay that relies upon fluorescence polarization is reported to indicate peptide interactions with recombinant soluble human leukocyte antigens (HLA) proteins. Testing of the A*0201-specific peptides with 48 additional class I molecules demonstrates that the unique peptide binding behavior of individual HLA molecules is maintained in the assay.

A novel state-of-the-art biochemical HLA-A*0201 assay, which is comprised of technologically advanced cutting edge reagents is developed and validated to better facilitate epitope discovery. Results obtained demonstrated significant correlation among assay procedures, suggesting that the application of fluorescence polarization in combination with recombinant sHLA molecules is highly advantageous for the accurate assessment of peptide binding.

The article shows that activated dendritic cells fail to cross-prime CD8+ T cell responses. Viruses that infect professional antigen-presenting cells may have contributed to the evolution of cross-priming. Cross-priming of CD8+ T cells involves three specialized features of dendritic cells. In vitro activation of dendritic cells results in inhibition of cross-priming.