1. Specific microRNA/mRNA expression profiles and novel immune regulation mechanisms are induced in THP‐1 macrophages by in vitro exposure to Trichosporon asahii
- Author
-
Mingwang Zhang, Xin Yang, Rongya Yang, Zhikuan Xia, and Junhong Ao
- Subjects
0301 basic medicine ,THP-1 Cells ,030106 microbiology ,Dermatology ,Trichosporon asahii ,Biology ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Trichosporonosis ,microRNA ,Humans ,RNA, Messenger ,KEGG ,Gene ,Messenger RNA ,Reporter gene ,Basidiomycota ,Macrophages ,Reproducibility of Results ,General Medicine ,Cell biology ,MicroRNAs ,Infectious Diseases ,Real-time polymerase chain reaction ,Gene Expression Regulation ,Signal Transduction - Abstract
Background Trichosporon asahii is considered the most prominent species associated with invasive trichosporonosis, but little is known about the pathogenesis of T. asahii infection in the host. MicroRNAs (miRNAs) are a class of noncoding endogenous small RNAs that play vital roles by manipulating immune responses against pathogenic microorganisms. Nevertheless, the exact functions of miRNAs in T. asahii infection are still unknown. Objective To investigate the interactions involved in the miRNA immune response in THP-1 macrophages following in vitro exposure to T. asahii. Methods We utilized next-generation sequencing to detect differentially expressed (DE) miRNAs and mRNAs in THP-1 cells after 24 h of in vitro exposure to T. asahii. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to verify the sequencing results. The miRNA-mRNA regulatory network was constructed with the DE miRNAs and DE mRNAs. We performed Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis of the predicted targeting mRNAs in the miRNA-mRNA network. A dual-luciferase reporter assay and enzyme-linked immunosorbent assay (ELISA) were utilized to demonstrate the reliability of the miR-342-3p/Dectin-1 pair. Results A total of 120 DE miRNAs and 588 DE mRNAs were identified after 24 h of in vitro exposure to T. asahii. The miRNA-mRNA regulatory network was constructed with 39 DE miRNAs and 228 DE mRNAs. KEGG pathway analysis revealed that the up-regulated DE mRNAs in the complex interaction network were mainly involved in immune-related pathways. In addition, we verified the target relationship between miR-342-3p and Dectin-1 and found that miR-342-3p could promote the expression of TNF-α and IL-6 by negatively regulating Dectin-1. Conclusions This study evaluated the expression profiles of miRNA/mRNA and revealed the immunological consequences of THP-1 macrophages in response to T. asahii exposure. Moreover, our data suggest that miR-342-3p can indirectly promote inflammatory responses and may be a potential therapeutic target against trichosporonosis.
- Published
- 2021