1. Tyramine induces dynamic RNP granule remodeling and translation activation in the Drosophila brain
- Author
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Florence Besse, Nadia Formicola, Anne-Sophie Lancelot, Mounia Lagha, Jeremy Dufourt, Akira Nakamura, Marjorie Heim, Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Institut de Génétique Moléculaire de Montpellier (IGMM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Kumamoto University, RUIZ, Caroline, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)
- Subjects
ZBP1 ,QH301-705.5 ,Science ,translation ,RNA-binding protein ,General Biochemistry, Genetics and Molecular Biology ,RNP condensates ,03 medical and health sciences ,0302 clinical medicine ,Ca2+/calmodulin-dependent protein kinase ,[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Biology (General) ,030304 developmental biology ,Ribonucleoprotein ,0303 health sciences ,D. melanogaster ,General Immunology and Microbiology ,biology ,Chemistry ,General Neuroscience ,Helicase ,RNA ,Translation (biology) ,Cell Biology ,General Medicine ,neuron ,Cell biology ,live-imaging ,biology.protein ,Medicine ,Translational Activation ,Drosophila ,RNA binding proteins ,030217 neurology & neurosurgery ,Research Article - Abstract
Ribonucleoprotein (RNP) granules are dynamic condensates enriched in regulatory RNA binding proteins (RBPs) and RNAs under tight spatiotemporal control. Extensive recent work has investigated the molecular principles underlying RNP granule assembly, unraveling that they form through the self-association of RNP components into dynamic networks of interactions. How endogenous RNP granules respond to external stimuli to regulate RNA fate is still largely unknown. Here, we demonstrate through high-resolution imaging of intact Drosophila brains that Tyramine induces a reversible remodeling of somatic RNP granules characterized by the decondensation of granule-enriched RBPs (e.g. Imp/ZBP1/IGF2BP) and helicases (e.g. Me31B/DDX-6/Rck). Furthermore, our functional analysis reveals that Tyramine signals both through its receptor TyrR and through the calcium-activated kinase CamkII to trigger RNP component decondensation. Finally, we uncover that RNP granule remodeling is accompanied by the rapid and specific translational activation of associated mRNAs. Thus, this work sheds new light on the mechanisms controlling cue-induced rearrangement of physiological RNP condensates.
- Published
- 2021