19 results on '"Guo-Jun Cai"'
Search Results
2. Antinociceptive activity of astragaloside IV in the animal model of chronic constriction injury
- Author
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Qi Wang, Rong Fan, Yu-Feng Chen, Yue Gao, Tong-chao Wang, Juan Song, Chen Yang, Guo-Jun Cai, Ming-Xiao Hou, Wei Zhang, and Guo-bing Shi
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Male ,Hot Temperature ,Neural Conduction ,Constriction, Pathologic ,Motor Activity ,Pharmacology ,Nerve conduction velocity ,Rats, Sprague-Dawley ,Necrosis ,Myelin ,Neurotrophic factors ,Ganglia, Spinal ,medicine ,Animals ,Analgesics ,business.industry ,Body Weight ,Saponins ,Sciatic Nerve ,Triterpenes ,Disease Models, Animal ,Psychiatry and Mental health ,Nociception ,medicine.anatomical_structure ,Allodynia ,Hyperalgesia ,Touch ,Anesthesia ,Neuropathic pain ,Neuralgia ,Sciatic nerve ,Chronic Pain ,medicine.symptom ,business - Abstract
To investigate the applicability of astragaloside IV (AG) for the treatment of refractory neuropathic pain, we systemically evaluated the antinociceptive activity of AG in the animal model of chronic constriction injury. We studied behaviors, electrophysiology, and biochemistry from day 2 to day 23 after the surgery. We found that when administered intraperitoneally at the dose of 60 mg/kg, AG caused significant inhibition of allodynia and hyperalgesia induced by mechanic and thermal stimuli as well as downregulation of the expressions of a series of proteins involved in mediating neuropathic pain in the dorsal root ganglia, such as P2X purinoceptor 3, glial cell-derived neurotrophic factor, glial cell-derived neurotrophic factor family receptor α1, and transient receptor potential cation channel subtypes A1 and V1. Further investigation showed that AG restored the nerve conduction velocity and the histological structure of the damaged sciatic nerve on day 23 after the surgery. Moreover, results from immunoelectron microscope showed that glial cell-derived neurotrophic factor family receptor α1 induced by AG could form a circular band in the myelin debris between the injured axons and Schwann cells, contributing toward restoration of the damaged nerve. In conclusion, in our animal model, AG effectively inhibited the neuropathic pain induced by chronic constriction injury.
- Published
- 2015
3. Possible Mechanism Involved in the Antinociceptive Activity of Dimer of Paederosidic Acid and Paederosidic Acid Methyl Ester in Mice
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Xin-Wei Wang, Dian-Hua Liu, Chen Yang, Yu-Feng Chen, Qiong Wu, Yu Yang, Guo-Jun Cai, and Jing-yi Zhang
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Pain Threshold ,Drug ,Time Factors ,Stereochemistry ,Dimer ,media_common.quotation_subject ,Formaldehyde ,Pain ,Heterocyclic Compounds, 2-Ring ,Mice ,chemistry.chemical_compound ,Glucosides ,Physiology (medical) ,Animals ,Pharmacology (medical) ,Letters to the Editor ,Pain Measurement ,media_common ,Pharmacology ,Analgesics ,Analysis of Variance ,Mice, Inbred ICR ,Dose-Response Relationship, Drug ,Morphine ,Chemistry ,Mechanism (biology) ,Paederosidic acid ,Disease Models, Animal ,Psychiatry and Mental health ,Dose–response relationship ,Nociception ,Biochemistry ,Hyperalgesia ,Paederosidic acid methyl ester - Published
- 2013
4. Antishock effect of anisodamine involves a novel pathway for activating α7 nicotinic acetylcholine receptor*
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Chong Liu, Ying-Ying Le, Ding-Feng Su, Xia Liu, Guo-Jun Cai, Fuming Shen, Alex F. Chen, and Yan Kong
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China ,medicine.medical_specialty ,alpha7 Nicotinic Acetylcholine Receptor ,Aconitine ,medicine.medical_treatment ,Interleukin-1beta ,Nicotinic Antagonists ,Receptors, Nicotinic ,Vagotomy ,Critical Care and Intensive Care Medicine ,Polymerase Chain Reaction ,Solanaceous Alkaloids ,Cell Line ,Anisodamine ,Proinflammatory cytokine ,Rats, Sprague-Dawley ,Mice ,chemistry.chemical_compound ,Intensive care ,Internal medicine ,Muscarinic acetylcholine receptor ,Animals ,Medicine ,Nicotinic Agonists ,Receptor ,Cholinergic anti-inflammatory pathway ,DNA Primers ,Acetylcholine receptor ,Base Sequence ,Tumor Necrosis Factor-alpha ,business.industry ,Shock, Septic ,Rats ,Endocrinology ,chemistry ,Macrophages, Peritoneal ,business ,Vagus nerve stimulation - Abstract
Vagus nerve stimulation inhibits proinflammatory cytokine production by signaling through the alpha7 nicotinic acetylcholine receptor (alpha7nAChR). Anisodamine, a muscarinic acetylcholine receptor antagonist, has been used clinically in China for treatment of various shocks, but the mechanism was poorly understood. Here, we tested the hypothesis whether anisodamine attained its antishock effect through activation of alpha7nAChR.: Randomized and controlled in vitro and in vivo study.Research laboratory and animal facility rooms.Sprague-Dawley rats, Kunming mice, alpha7nAChR-deficient mice, and RAW264.7 cells.Sprague-Dawley rats were injected with lipopolysaccharide (LPS) (15 mg/kg, intravenous) to induce septic shock. Methyllycaconitine, a selective alpha7nAChR antagonist, was administered (10 mg/kg, intraperitoneal) 10 minutes before anisodamine (10 mg/kg, intravenous). Mean arterial pressure was monitored and cytokines were analyzed 2 hours after the onset of LPS. In vagotomized mice and alpha7nAChR-deficient mice, the antishock effect of anisodamine was appraised, respectively. RAW264.7 cells were stained by fluorescein isothiocyanate- labeled-alpha-bungarotoxin and the fluorescence intensity was observed. Mice peritoneal macrophages were pretreated and stimulated with LPS, and tumor necrosis factor (TNF)-alpha in the supernatant was measured by enzyme-linked immunosorbent assay.Methyllycaconitine significantly antagonized the beneficial effect of anisodamine on mean arterial pressure and TNF-alpha, interleukin-1beta expression in response to LPS. The antishock effects of anisodamine were markedly attenuated in vagotomized mice and alpha7nAChR-deficient mice. In vitro, anisodamine significantly augmented the effect of acetylcholine on fluorescence intensity stained with fluorescein isothiocyanate-labeled-alpha-bungarotoxin and TNF-alpha production stimulated with LPS.These findings demonstrate that the antishock effect of anisodamine is intimately linked to alpha7nAChR-dependent anti-inflammatory pathway.
- Published
- 2009
5. Arterial Baroreflex Dysfunction Fails to Mimic Parkinson’s Disease in Rats
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Fu-Ming Shen, Ding-Feng Su, Jian-Guo Liu, Jian-Guang Yu, Jian Wu, and Guo-Jun Cai
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Male ,medicine.medical_specialty ,Parkinson's disease ,Dopamine ,Blood Pressure ,Striatum ,Baroreflex ,Rats, Sprague-Dawley ,Lesion ,chemistry.chemical_compound ,Heart Rate ,Rotenone ,Internal medicine ,Animals ,Medicine ,Parkinson Disease, Secondary ,Oxidopamine ,Sinoatrial Node ,Pharmacology ,Catalepsy ,Behavior, Animal ,business.industry ,Dopaminergic ,lcsh:RM1-950 ,medicine.disease ,Denervation ,Corpus Striatum ,Rats ,Blood pressure ,Endocrinology ,lcsh:Therapeutics. Pharmacology ,chemistry ,Molecular Medicine ,medicine.symptom ,business ,medicine.drug - Abstract
Patients with Parkinson’s disease (PD) often have attenuated baroreflex function, which may occur before the onset of PD-associated movement disorders. The aim of the present study was to test whether impaired arterial baroreflex (ABR) function could contribute to the pathogenesis of PD. 6-Hydroxydopamine (8 μg in 4 μl) was microinjected into the left substantia nigra of rats to establish unilateral PD models, and bilateral PD models were established in rats by administration of rotenone by osmotic minipump for four weeks, at a dose of 2.5 mg ⋅ kg−1 ⋅ day−1. An ABR dysfunction model was obtained by performing sinoaortic denervation (SAD). Hemodynamic variables were determined in conscious rats. PD-like symptoms and dopamine content in corpus striatum (CS) were also assessed. 6-Hydroxydopamine and rotenone treatment and SAD were associated with enhanced blood pressure variability (BPV) and blunted baroreflex sensitivity (BRS). Rotenone, but not SAD, significantly reduced dopamine content in the CS, induced catalepsy, and inhibited rearing and exploratory behavior. SAD before the administration of rotenone did not aggravate the rotenone-induced dopaminergic lesion. Our findings do not support the presumption that ABR dysfunction contributes to the pathogenesis of PD in rats. Keywords:: arterial baroreflex, Parkinson’s disease, baroreflex sensitivity, blood pressure variability, sinoaortic denervation
- Published
- 2008
6. EXPRESSION OF P2X PURINOCEPTORS IN PC12 PHAEOCHROMOCYTOMA CELLS
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Zheng-Hua Xiang, Ji-Hu Sun, and Guo-Jun Cai
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endocrine system ,Patch-Clamp Techniques ,Physiology ,PC12 cell line ,Alpha (ethology) ,Biology ,PC12 Cells ,chemistry.chemical_compound ,Physiology (medical) ,Nerve Growth Factor ,Animals ,RNA, Messenger ,Patch clamp ,Receptor ,Pharmacology ,Receptors, Purinergic P2 ,musculoskeletal, neural, and ocular physiology ,Molecular biology ,Rats ,Reverse transcription polymerase chain reaction ,Electrophysiology ,Nerve growth factor ,nervous system ,chemistry ,Receptors, Purinergic P2X ,Immunohistochemistry - Abstract
1. The PC12 cell line, which was cloned from a rat adrenal phaeochromocytoma, is a useful model system. It expresses neuronal properties after treatment with nerve growth factor (NGF). The nervous system-specific P2X receptor subtype P2X(2) was initially cloned from PC12 cells, but little is known about the expression of other subtypes of P2X receptors in PC12 cells. The aim of the present study was to investigate whether PC12 cells express the other P2X receptors when exposed to NGF. 2. Reverse transcription-polymerase chain reaction at the mRNA level and immunocytochemisty at the protein level showed that, among the seven P2X purinoceptor subtypes, only P2X(2) was found to be expressed in undifferentiated PC12 phaeochromocytoma cells, but all seven P2X purinoceptor subtypes were expressed in differentiated PC12 cells treated with 50 microg/mL NGF. 3. Electrophysiological recordings indicated that ATP (30 micromol/L) but not alpha,beta-methylene ATP (alpha,beta-meATP; 30 micromol/L) evoked an inward current in undifferentiated PC12 cells, but both alpha,beta-meATP and ATP evoked inward currents in differentiated PC12 cells. The results indicate that the NGF-induced P2X receptors expressed in PC12 cells are functional channels. 4. The present study suggests that the NGF-induced neuronal phenotype of PC12 cells may be a model for the study of P2X heteromeric receptors.
- Published
- 2007
7. Action site of ketanserin enhancing baroreflex function is within the rostral ventrolateral medulla in anesthetized rats
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Min-Wei Wang, Wei-Zhong Wang, Yan-Jun Fu, Ding-Feng Su, and Guo-Jun Cai
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Male ,medicine.medical_specialty ,Ketanserin ,Microinjections ,Hemodynamics ,Blood Pressure ,Baroreflex ,Rats, Sprague-Dawley ,Phenylephrine ,Cellular and Molecular Neuroscience ,Heart Rate ,Internal medicine ,Solitary Nucleus ,medicine ,Animals ,Anesthesia ,Peripheral Nerves ,Antihypertensive Agents ,Medulla Oblongata ,Endocrine and Autonomic Systems ,business.industry ,musculoskeletal, neural, and ocular physiology ,fungi ,Rostral ventrolateral medulla ,Electric Stimulation ,Rats ,Autonomic nervous system ,Blood pressure ,Endocrinology ,nervous system ,cardiovascular system ,Medulla oblongata ,Neurology (clinical) ,business ,Adrenergic alpha-Agonists ,circulatory and respiratory physiology ,medicine.drug - Abstract
In our previous studies, it was found that ketanserin enhanced baroreflex sensitivity (BRS) in rats and this effect was not blood pressure dependent. The present work was designed to investigate the effects of ketanserin on BRS within the nucleus tractus solitarus (NTS) and the rostral ventrolateral medulla (RVLM). In anesthetized rats, BRS were evaluated by the changes in depressor action of aortic nerve stimulation and bradycardiac response to rapid pressor action of intravenous phenylephrine. It was found that bilateral injection of ketanserin (250 pmol for each side) into the NTS not only significantly increased blood pressure, but also attenuated baroreflex function. Interestingly, when ketanserin was bilaterally injected into RVLM, a significant enhancement of BRS was found and there were no modifications in basal blood pressure and heart period. The present study demonstrates that ketanserin-induced enhancement of BRS mainly occurred within the RVLM, which might contribute to the systemic effects of ketanserin on BRS.
- Published
- 2006
8. Diminished Responses to Nifedipine Imply Severe End-Organ Damage in Spontaneously Hypertensive Rats
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Ding-Feng Su, Fu-Ming Shen, He-Hui Xie, Guo-Jun Cai, and Li-Hua Lu
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Male ,medicine.medical_specialty ,Nifedipine ,End organ damage ,Diastole ,Blood Pressure ,Baroreflex ,Kidney ,Muscle hypertrophy ,Basal (phylogenetics) ,Rats, Inbred SHR ,Internal medicine ,medicine ,Animals ,Left kidney ,Aorta ,Pharmacology ,business.industry ,Body Weight ,Organ Size ,Calcium Channel Blockers ,medicine.disease ,Rats ,Blood pressure ,Endocrinology ,Hypertension ,Regression Analysis ,Cardiology and Cardiovascular Medicine ,business ,medicine.drug - Abstract
This study was designed to investigate the effect of end-organ damage (EOD), the initial blood pressure levels, and baroreflex sensitivity (BRS) on the blood pressure-lowering effect of nifedipine in spontaneously hypertensive rats (SHRs). Nifedipine was intravenously administered at a dose of 1 mg/kg. BP was continuously recorded in the conscious state before and after nifedipine administration. BRS was determined before drug administration. Two days after the blood pressure (BP) recording, rats were killed for organ-damage evaluation. Univariate correlation analysis showed that BP changes induced by nifedipine injection were negatively correlated with EOD score and aortic weight/length but positively correlated with left kidney weight/body weight and basal BP levels. Stepwise multiple linear regression analysis demonstrated that increase in overall end-organ damage was most significantly related to the decrease in hypotensive effect of nifedipine; increase in aortic hypertrophy was also related to a decreased fall in systolic and diastolic BP induced by nifedipine, whereas increase in initial BP levels was associated with increased hypotensive effect of nifedipine. In conclusion, the severity of overall EOD contributed more than basal BP levels to the diminished responses to nifedipine, and aortic hypertrophy was also involved in diminished drug responses.
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- 2005
9. GREATER HYPERTROPHY IN RIGHT THAN LEFT VENTRICLES IS ASSOCIATED WITH PULMONARY VASCULOPATHY IN SINOAORTIC-DENERVATED WISTAR-KYOTO RATS
- Author
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Xia Tao, Chao-Yu Miao, Guo-Jun Cai, He-Hui Xie, and Ding-Feng Su
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Male ,medicine.medical_specialty ,Baroreceptor ,Physiology ,Left Ventricles ,Rats, Inbred WKY ,Muscle hypertrophy ,Right ventricular hypertrophy ,Physiology (medical) ,Internal medicine ,medicine.artery ,medicine ,Animals ,cardiovascular diseases ,Lung ,Sinoatrial Node ,Pharmacology ,Hypertrophy, Right Ventricular ,business.industry ,Body Weight ,Hemodynamics ,Organ Size ,medicine.disease ,Denervation ,Rats ,medicine.anatomical_structure ,Blood pressure ,Ventricle ,Pulmonary artery ,Linear Models ,cardiovascular system ,Cardiology ,Blood Vessels ,Hypertrophy, Left Ventricular ,business - Abstract
SUMMARY 1. Biventricular hypertrophy has been described in a high blood pressure variability (BPV) model of sinoaortic-denervated (SAD) rats without systemic hypertension. To explore the possible involvement of the lung in SAD-induced right ventricular hypertrophy (RVH), we examined lung morphology, in addition to systemic haemodynamics and ventricle morphology, in Wistar-Kyoto rats 32 weeks after SAD. 2. In Wistar-Kyoto rats 32 weeks after SAD, there existed a substantial elevation in BPV, with no change in the average level of arterial pressure. Biventricular hypertrophy following SAD was characterized by a greater hypertrophy in right than left ventricles; both absolute and normalized right ventricular weights were significantly increased by 22 and 27%, respectively, and only normalized left ventricular weight was significantly increased by 12%. No infarcts were found in any ventricles examined. 3. In the lung, the most prominent change following SAD was pulmonary vasculopathy, including wall thickening, perivascular fibrosis and cell infiltration. In pulmonary arteries with an internal diameter of 70–130 µm, the external diameter, wall thickness and wall thickness to internal diameter ratio were increased in SAD compared with control rats. 4. There was no correlation between right and left ventricular weights. In contrast with BPV-correlated left ventricular weight, right ventricular weight was correlated with the wall thickness of the pulmonary artery, but not with BPV. 5. These findings suggest that greater RVH following SAD is associated with pulmonary vasculopathy, but is not secondary to the left ventricular problems or high BPV.
- Published
- 2004
10. Ketanserin improves cardiac performance after myocardial infarction in spontaneously hypertensive rats partially through restoration of baroreflex function
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Ding-Feng Su, Jian-Guo Liu, Ai-Jun Liu, En-hui Zhang, Guo-Jun Cai, and Jian-Guang Yu
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Cardiac function curve ,Vascular Endothelial Growth Factor A ,medicine.medical_specialty ,Ketanserin ,Myocardial Infarction ,Enzyme-Linked Immunosorbent Assay ,Baroreflex ,Sudden death ,Internal medicine ,Rats, Inbred SHR ,Heart rate ,medicine ,Animals ,Pharmacology (medical) ,cardiovascular diseases ,Myocardial infarction ,Pharmacology ,Ejection fraction ,business.industry ,musculoskeletal, neural, and ocular physiology ,Heart ,General Medicine ,medicine.disease ,Acetylcholine ,Rats ,Blood pressure ,Endocrinology ,cardiovascular system ,Original Article ,business ,circulatory and respiratory physiology ,medicine.drug - Abstract
Baroreflex dysfunction is associated with a higher rate of sudden death after myocardial infarction (MI). Ketanserin enhances baroreflex function in rats. The present work was designed to examine whether ketanserin improves the post-MI cardiac function and to explore the possible mechanism involved. Spontaneously hypertensive rats (SHR) were treated with ketanserin (0.3 mg·kg−1·d−1). Two weeks later, blood pressure and baroreflex function were measured, followed by a ligation of the left coronary artery. The expressions of vesicular acetylcholine transporter (VAChT) and α7 nicotinic acetylcholine receptor (α7-nAChR) in ischemic myocardium, angiogenesis, cardiac function, and left ventricular (LV) remodeling were evaluated subsequently. Ketanserin significantly improved baroreflex sensitivity (0.62±0.21 vs 0.34±0.12 ms/mmHg, P
- Published
- 2013
11. ATP13A2 Knockout Does not Affect the Infarct Size in Mice with Acute Ischemic Stroke
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Jian-Guang Yu, Mei-Jian Cui, Kai-Bing Hu, Guo-Jun Cai, and Wei Wang
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Mitochondrial ROS ,Brain Infarction ,Programmed cell death ,medicine.medical_specialty ,Excitotoxicity ,Apoptosis ,medicine.disease_cause ,Mice ,Physiology (medical) ,Internal medicine ,medicine ,Animals ,Humans ,Pharmacology (medical) ,Letters to the Editor ,Stroke ,Pharmacology ,Mice, Knockout ,business.industry ,Caspase 3 ,Penumbra ,Autophagy ,Infarction, Middle Cerebral Artery ,medicine.disease ,Psychiatry and Mental health ,Disease Models, Animal ,Proton-Translocating ATPases ,Endocrinology ,Gene Expression Regulation ,Knockout mouse ,Neuron death ,business ,Microtubule-Associated Proteins - Abstract
The loss-of-function mutations of the human gene ATP13A2, which encodes a lysosomal type 5 P-type ATPase of unknown function, were found to underlie an autosomal recessive form of early-onset parkinsonism (Kufor-Rakeb Syndrome) [1]. It was reported that autophagy is involved in the pathogenesis of many chronic neurodegenerative diseases, as well as acute brain damage such as stroke [2,3]. Recently, Gusdon et al. found that ATP13A2 regulates mitochondrial bioenergetics through macroautophagy [4], which is a major response to nutrient and bioenergetic stresses, with the capacity to remove aggregated proteins and damaged organelles such as mitochondria. They proposed that decreased autophagy associated with ATP13A2 deficiency affects mitochondrial quality control, resulting in increased production of reactive oxygen species (ROS) [4]. As known, necrotic cell death as a rapid cellular response involves mitochondrial ROS production and other cellular insults, whereas autophagic cell death first attempts to clean up ROSdamaged mitochondria for survival. Stroke is the second leading cause of death in the world and the primary cause of adult disability [5]. Ischemic stroke accounts for 80% of all the cases. In ischemic stroke, neuron death occurs via necrosis and apoptosis, as well as autophagic cell death. In this study, we explored the influence of ATP13A2 knockout on the infarct size in mice with ischemic stroke, as well as the possible influence on apoptosis and autophagy involved in ischemic stroke. ATP13A2 knockout mice and corresponding wild-type mice were subjected to middle cerebral artery occlusion (MCAO) as previously described [6,7], without reperfusion. After 24 h, apoptosis/autophagy-related protein levels in infarct penumbra of cerebral cortex were detected through Western blot [8,9]. Data are expressed as mean ± SD. Differences between the two groups were evaluated by unpaired t-test. As known, formation of autophagosome requires the cooperation of microtubule-associated protein light chain 3 (LC3). LC3 is converted into LC3-I and then LC3-II, which is the marker of autophagosome. Our results showed that ATP13A2 knockout increased the level of LC3-II in infarct penumbra (by 16.1%, Figure 1A). Furthermore, the expression of pro-apoptotic protein Bax and apoptosis executer caspase-3 was increased in ATP13A2 knockout mice (by 12.9% and 33.6% respectively), while the expression of antiapoptotic protein Bcl-2 has no significant difference between two groups, as shown in Figure 1B–D. To test the influence of ATP13A2 knockout on the infarct size, mice were sacrificed 24 h after MCAO. Brain slices were prepared and stained with 1% 2,3,5-triphenyltetrazolium chloride (TTC). The infarct size was measured with an image analyzer. Our results showed that there was no significant difference in infarct size between ATP13A2 knockout mice and corresponding wild-type mice, as shown in Figure 2. Studies have shown that the pathophysiology of ischemic stroke involves a number of mechanisms, including necrosis, apoptosis, inflammation, excitotoxicity, and free radical damage. In this study, we found that both apoptosis and autophagy were increased in ATP13A2 knockout mice. In fact, when autophagy occurs in excess, it becomes cytotoxic and eventually leads to autophagic cell death and apoptosis [10]. However, the infarct size
- Published
- 2012
12. The protective action of ketanserin against lipopolysaccharide-induced shock in mice is mediated by inhibiting inducible NO synthase expression via the MEK/ERK pathway
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Wei Wei, Ping Ke, Ding-Feng Su, Gufang Zhang, Dian-Hua Liu, Guo-Jun Cai, Chong Liu, Jv-Xiang Zhou, and Xin Zhang
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MAPK/ERK pathway ,Lipopolysaccharides ,Male ,medicine.medical_specialty ,Ketanserin ,Lipopolysaccharide ,medicine.drug_class ,MAP Kinase Signaling System ,Blotting, Western ,Nitric Oxide Synthase Type II ,Ritanserin ,Electrophoretic Mobility Shift Assay ,Enzyme-Linked Immunosorbent Assay ,Transfection ,Biochemistry ,Nitric oxide ,chemistry.chemical_compound ,Mice ,Physiology (medical) ,Internal medicine ,medicine ,Animals ,RNA, Small Interfering ,Receptor ,Antihypertensive Agents ,Receptor antagonist ,Immunohistochemistry ,Shock, Septic ,Disease Models, Animal ,Endocrinology ,chemistry ,Signal transduction ,medicine.drug - Abstract
Nitric oxide (NO) plays an important role in the pathogenesis of endotoxic shock. This work tested the hypothesis that ketanserin could attenuate endotoxic shock by inhibiting the expression of inducible NO synthase (iNOS). The results demonstrated that ketanserin could inhibit iNOS expression in the heart, lungs, liver, and kidneys and nitrate production in the serum upon endotoxic shock in mice. In RAW264.7 cells, ketanserin significantly inhibited the expression of iNOS and decreased the production of NO, TNFα, IL-6, and reactive oxygen species upon lipopolysaccharide (LPS) challenge. Ketanserin also increased the level of ATP and mitochondrial membrane potential in RAW264.7 cells upon LPS exposure. LPS-induced iNOS expression was inhibited by the 5-HT2A receptor antagonist ritanserin and not the α1 receptor antagonist prazosin. Knockdown of 5-HT2A receptor by siRNA abolished the inhibitory effect of ketanserin on the expression of iNOS. These results indicated that the inhibitory effect of ketanserin on the expression of iNOS is mediated by blocking the 5-HT2A receptor. Furthermore, ketanserin significantly inhibited the activation of ERK1/2 and NF-κB signal. Pretreatment with PD184352, a specific inhibitor of ERK1/2, blocked the inhibitory effect of ketanserin on the expression of iNOS and NO production, indicating a critical role for the MEK/ERK1/2 signaling pathway. Collectively, our findings indicate that inhibition of the expression of iNOS via the MEK/ERK pathway mediates the protective effects of ketanserin against LPS-induced shock in mice.
- Published
- 2012
13. Effects of ketanserin on endotoxic shock and baroreflex function in rodents
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Gufang Zhang, Chong Liu, Ding-Feng Su, Chao-Yu Miao, Wen-Hao Liu, Guo-Jun Cai, and Shu-Wei Song
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Lipopolysaccharides ,Male ,Ketanserin ,Lipopolysaccharide ,medicine.drug_class ,Interleukin-1beta ,Blood Pressure ,Pharmacology ,Baroreflex ,Rats, Inbred WKY ,chemistry.chemical_compound ,Mice ,Heart Rate ,medicine ,Immunology and Allergy ,Animals ,Dose-Response Relationship, Drug ,business.industry ,Tumor Necrosis Factor-alpha ,musculoskeletal, neural, and ocular physiology ,Interleukin ,musculoskeletal system ,Receptor antagonist ,Shock, Septic ,Survival Analysis ,Interleukin-10 ,Rats ,Dose–response relationship ,Infectious Diseases ,Blood pressure ,chemistry ,Shock (circulatory) ,Anesthesia ,cardiovascular system ,Serotonin Antagonists ,medicine.symptom ,business ,circulatory and respiratory physiology ,medicine.drug - Abstract
Background Ketanserin, a 5-hydroxytryptamine receptor antagonist, is clinically used as an antihypertensive agent and could enhance baroreflex function. The present work tested the hypothesis that restoration of baroreflex function is an effective treatment for lipopolysaccharide (LPS)-induced shock. Methods Kunming mice were injected with LPS (30 mg/kg; intraperitoneal) to induce endotoxic shock. Ketanserin (0.3, 1, 3, or 10 mg/kg; intraperitoneal) was administered immediately after LPS injection. Survival time was monitored, and serum cytokines were analyzed after the onset of LPS. Effects of ketanserin were also examined in IL-10-deficient mice and mice with sinoaortic denervation. Finally, effects of ketanserin on blood pressure, heart rate, and baroreflex sensitivity were examined in Wistar-Kyoto (WKY) rats with endotoxic shock. Results Ketanserin significantly increased survival time and decreased serum levels of tumor necrosis factor α and interleukin (IL) 1β in mice with endotoxic shock. At a dose of 10 mg/kg, ketanserin also significantly increased serum IL-10 concentration. The antishock effect of ketanserin was also apparent in IL-10-knockout mice. In mice with sinoaortic denervation, however, ketanserin had little antishock effects. In WKY rats, ketanserin significantly prevented the baroreflex impairment induced by LPS and prolonged the survival time. Conclusions Ketanserin could ameliorate endotoxic shock by restoring baroreflex function.
- Published
- 2011
14. Interaction between age and obesity on cardiomyocyte contractile function: role of leptin and stress signaling
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Jennifer M. Nunn, Feng Dong, Guo-Jun Cai, Peng Zhao, Jianming Pei, Jun Ren, and Loren E. Wold
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Leptin ,Male ,medicine.medical_specialty ,Aging ,Nitric Oxide Synthase Type III ,lcsh:Medicine ,Mice, Obese ,030204 cardiovascular system & hematology ,Diabetes and Endocrinology/Obesity ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Downregulation and upregulation ,Enos ,Stress, Physiological ,Superoxides ,Internal medicine ,medicine ,Animals ,Myocytes, Cardiac ,Calcium Signaling ,Obesity ,lcsh:Science ,Protein kinase B ,Cells, Cultured ,030304 developmental biology ,2. Zero hunger ,0303 health sciences ,Multidisciplinary ,Leptin receptor ,biology ,lcsh:R ,biology.organism_classification ,Dietary Fats ,Myocardial Contraction ,Geriatrics/Geriatric Cardiology ,Endocrinology ,Phosphorylation ,Cardiovascular Disorders/Myopathies ,lcsh:Q ,Signal transduction ,Proto-Oncogene Proteins c-akt ,Intracellular ,Research Article ,Signal Transduction - Abstract
OBJECTIVES This study was designed to evaluate the interaction between aging and obesity on cardiac contractile and intracellular Ca2+ properties. METHODS Cardiomyocytes from young (4-mo) and aging (12- and 18-mo) male lean and the leptin deficient ob/ob obese mice were treated with leptin (0.5, 1.0 and 50 nM) for 4 hrs in vitro. High fat diet (45% calorie from fat) and the leptin receptor mutant db/db obesity models at young and older age were used for comparison. Cardiomyocyte contractile and intracellular Ca2+ properties were evaluated including peak shortening (PS), maximal velocity of shortening/relengthening (+/- dL/dt), time-to-PS (TPS), time-to-90% relengthening (TR(90)), intracellular Ca2+ levels and decay. O2(-) levels were measured by dihydroethidium fluorescence. RESULTS Our results revealed reduced survival in ob/ob mice. Aging and obesity reduced PS, +/- dL/dt, intracellular Ca2+ rise, prolonged TR(90) and intracellular Ca2+ decay, enhanced O2(-) production and p(47phox) expression without an additive effect of the two, with the exception of intracellular Ca2+ rise. Western blot analysis exhibited reduced Ob-R expression and STAT-3 phosphorylation in both young and aging ob/ob mice, which was restored by leptin. Aging and obesity reduced phosphorylation of Akt, eNOS and p38 while promoting pJNK and pIkappaB. Low levels of leptin reconciled contractile, intracellular Ca2+ and cell signaling defects as well as O2(-) production and p(47phox) upregulation in young but not aging ob/ob mice. High level of leptin (50 nM) compromised contractile and intracellular Ca2+ response as well as O2(-) production and stress signaling in all groups. High fat diet-induced and db/db obesity displayed somewhat comparable aging-induced mechanical but not leptin response. CONCLUSIONS Taken together, our data suggest that aging and obesity compromise cardiac contractile function possibly via phosphorylation of Akt, eNOS and stress signaling-associated O2(-) release.
- Published
- 2009
15. Effects of combination therapy with atenolol and amlodipine on blood pressure control and stroke prevention in stroke-prone spontaneously hypertensive rats
- Author
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Guo-Jun Cai, Ding-Feng Su, Fu-Ming Shen, Ai-Jun Liu, Jian-Guo Liu, and Gang Ling
- Subjects
Male ,Combination therapy ,Diastole ,Blood Pressure ,Baroreflex ,Rats, Inbred SHR ,Medicine ,Animals ,Pharmacology (medical) ,cardiovascular diseases ,Amlodipine ,Stroke ,Antihypertensive Agents ,Pharmacology ,business.industry ,Drug Synergism ,General Medicine ,medicine.disease ,Atenolol ,Rats ,Survival Rate ,Blood pressure ,Anesthesia ,Decreased blood pressure ,Drug Therapy, Combination ,Female ,business ,medicine.drug - Abstract
Aim: To test the effects of atenolol and amlodipine, either alone or in combination, on blood pressure, blood pressure variability (BPV), baroreflex sensitivity (BRS), and the prevalence of stroke in stroke-prone spontaneously hypertensive rats (SHR-SP). Methods: In the first set of the study, 24 8-month-old, female SHR-SP rats were randomly divided into 3 groups. Blood pressure, heart period, and BRS were determined before and after the intragastric administration of atenolol (10 mg/kg) and amlodipine (1.0 mg/kg), either alone or in combination. In the second set of the study, 40 male and 40 female rats were randomly assigned to 1 of the following 4 groups: control, atenolol (10 mg·kg -1 ·d -1 ), amlodipine (1.0 mg·kg -1 ·d -1 ), and both (10 male and 10 female in each group). The stroke incident and survival time were recorded. Results: Atenolol and amlodipine, either alone or in combination, significantly decreased blood pressure, with the exception of the amlodipine-induced effect on diastolic blood pressure. Meanwhile, only the combination treatment significantly decreased the BPV levels for the same period. The q-values calculated by the probability sum analysis were 1.17 and 2.67 for systolic and diastolic blood pressure, respectively, and were 2.48 and 2.10 for systolic and diastolic BPV, respectively, following administration. Neither drug exhibited any significant effect on BRS. Atenolol and amlodipine, either alone or in combination, significantly increased the lifespan of SHR-SP, with the best effect elicited by the combination therapy. Conclusion: A significant synergism exists between atenolol and amlodipine in lowering and stabilizing blood pressure in SHR-SP. Combination therapy may be an optimal strategy for the prevention of stroke in hypertension.
- Published
- 2007
16. A novel animal model for motion sickness and its first application in rodents
- Author
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Zheng-Xu Chu, Ding-Feng Su, Guo-Jun Cai, Xu-Hong Yu, and Ai-Jun Liu
- Subjects
Male ,Ratón ,Motion Sickness ,Scopolamine ,Urination ,Experimental and Cognitive Psychology ,Modafinil ,Muscarinic Antagonists ,Pharmacology ,Parasympatholytic ,Rats, Sprague-Dawley ,Behavioral Neuroscience ,Mice ,Animal model ,Tremor ,medicine ,Distribution (pharmacology) ,Animals ,Benzhydryl Compounds ,Defecation ,Dose-Response Relationship, Drug ,business.industry ,Alkaloid ,medicine.disease ,Piloerection ,Rats ,Disease Models, Animal ,Motion sickness ,Anesthesia ,Central Nervous System Stimulants ,Drug Therapy, Combination ,Female ,business ,medicine.drug - Abstract
The present work was designed to establish a novel animal model for motion sickness (MS) in rodents and to evaluate the effects of a combination of scopolamine and modafinil on MS with this novel method. It was found that the rats and mice presented several symptoms induced by rotation such as, piloerection, tremble, urinal and fecal incontinence. As the rats and mice are lack of emesis response to rotation, we used a score based on abovementioned symptoms as an index for the severity of MS in rodents. MS index was determined in 260 mice with this novel method. It was found that the distribution of MS index was normal (W=0.99; P=0.23. P>0.05 considered values' normal distribution). The effects of scopolamine on MS were studied in mice and rats. It was found that scopolamine significantly decreased MS index at the dose of 0.3 mg/kg in mice and 1.0 mg/kg in rats. Finally, the effects of a combination of scopolamine and modafinil were observed with this novel method in rats. It was found that the efficacy of the combination (5.0+5.0 mg/kg) was greater than the single drugs (10 mg/kg). Even the smallest dose of the combination (0.5+0.5 mg/kg) had a similar effect to large dose of scopolamine or modafinile when they were used alone. In conclusion, this animal model is suitable for MS study in rats and mice and the combination of scopolamine and modafinil might be a new method to treat or prevent MS.
- Published
- 2007
17. Gradual upregulation of OCI-5 expression during occurrence and progression of rat hepatocellular carcinoma
- Author
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Wei-Jun, Jiang, Xiao-Bo, Man, Liang, Tang, Hong-Yin, Song, Shen-Jing, Li, Guo-Jun, Cai, Xiu-Hua, Qiu, and He-Ping, Hu
- Subjects
Male ,Carcinoma, Hepatocellular ,Base Sequence ,Reverse Transcriptase Polymerase Chain Reaction ,Molecular Sequence Data ,Sensitivity and Specificity ,Rats ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,Rats, Sprague-Dawley ,Disease Models, Animal ,Liver Neoplasms, Experimental ,Glypicans ,Disease Progression ,Tumor Cells, Cultured ,Animals ,RNA ,Heparan Sulfate Proteoglycans - Abstract
OCI-5, the rat homologene of human glypican 3 (GPC3), is confirmed upregulated in hepatocellular carcinoma (HCC). The present study was undertaken to detect gene expression change of OCI-5 during occurrence and progression of rat HCC.Male Sprague-Dawley rats were given diethylnitrosamine (DENA) to induce HCC. Three DENA-induced rats and one control rat were sacrificed every week for 18 weeks during the development of HCC. Tissues specimens were snap-frozen in liquid nitrogen and total RNA was isolated. Sk-Hep1 cells were treated with DENA at different concentrations. The gene expression levels of OCI-5 and GPC3 were detected with the RT-PCR method.OCI-5 was not expressed in normal rat liver tissues. When HCC occurred and aggravated, OCI-5 expression was gradually elevated to a very high level. GPC3 was not expressed in the DENA-treated Sk-Hep1 cells.OCI-5 was not expressed in normal rat liver tissues but in rat HCC tissues. High-expression of OCI-5 in DENA-induced rat HCC model was the gene expression change of HCC not the DENA-induced gene expression. The expression level of OCI-5 was not only elevated in rat HCC but also gradually along the occurrence and progression of HCC, indicating that GPC3 might serve as a sensitive marker of early stage HCC.
- Published
- 2006
18. Blood pressure variability, cardiac baroreflex sensitivity and organ damage in experimentally hypertensive rats
- Author
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Di-Song, Wang, He-Hui, Xie, Fu-Ming, Shen, Guo-Jun, Cai, and Ding-Feng, Su
- Subjects
Male ,Time Factors ,Endothelin-1 ,Tumor Necrosis Factor-alpha ,Angiotensin II ,Myocardium ,Kidney Glomerulus ,Blood Pressure ,Blood Pressure Determination ,Organ Size ,Baroreflex ,Rats ,Rats, Sprague-Dawley ,Disease Models, Animal ,Hypertension, Renovascular ,NG-Nitroarginine Methyl Ester ,Hypertension ,Animals ,Desoxycorticosterone ,Interleukin-1 - Abstract
1. The present study was designed to investigate the haemodynamic features and morphological changes in experimentally hypertensive rat models. 2. Sprague-Dawley rats were used to prepare the experimentally hypertensive models, including two-kidney, one-clip renovascular hypertensive (2K1C) rats, deoxycorticosterone acetate salt hypertensive (DOCA) rats and N(G)-nitro-l-arginine methyl ester-induced hypertensive (l-NAME) rats. Six weeks after the induction of hypertension, 24 h blood pressure was recorded and blood pressure variability (BPV) expressed by 24 h (or 12 h in the daytime and night-time study) standard deviation of the variables was calculated. Then, cardiac baroreflex sensitivity (BRS) was determined and four endogenous factors (tumour necrosis factor-alpha, interleukin-1beta, angiotensin II and endothelin-1) were measured. Finally, morphological changes were examined. 3. It was found that an increase in BPV and a decrease in BRS were accompanied by an elevation of blood pressure in all three hypertensive models. The DOCA rats had the highest BPV, whereas the l-NAME rats had the lowest BRS. 4. Morphological changes were similar in DOCA and l-NAME rats and the cardiac changes were relatively slight in 2K1C rats. Tumour necrosis factor-alpha was increased in all the three models, especially in DOCA rats. Endothelin-1 was higher in DOCA rats and angiotensin II was increased in 2K1C rats and decreased in DOCA rats. 5. In conclusion, increased BPV and decreased BRS accompanied the elevation of blood pressure in all three hypertensive models. The DOCA rats had the highest BPV and the l-NAME rats had the lowest BRS. Obvious organ damage was seen in all three hypertensive models 6 weeks after the induction of hypertension.
- Published
- 2005
19. Arterial baroreflex dysfunction promotes atherosclerosis in rats
- Author
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Chao-Yu Miao, Ding-Feng Su, He-Hui Xie, Guo-Jun Cai, and Li-Hua Lu
- Subjects
Male ,medicine.medical_specialty ,Hypercholesterolemia ,Aortic Diseases ,Hemodynamics ,Vascular Cell Adhesion Molecule-1 ,Baroreflex ,Rats, Sprague-Dawley ,Phenylephrine ,medicine.artery ,Internal medicine ,Autonomic Denervation ,Medicine ,Animals ,Aorta ,Cholecalciferol ,Aortic atherosclerosis ,Reflex, Abnormal ,business.industry ,Myocardium ,Atherosclerosis ,Intercellular Adhesion Molecule-1 ,Rats ,Blood pressure ,Endocrinology ,medicine.anatomical_structure ,C-Reactive Protein ,Carotid Arteries ,Circulatory system ,Hypertension ,Diet, Atherogenic ,Cardiology and Cardiovascular Medicine ,business ,Blood vessel ,Artery - Abstract
The present study was designed to test the hypothesis that arterial baroreflex dysfunction promotes the development of atherosclerosis. Experiment 1: the baroreflex sensitivity (BRS) was measured in 30 Sprague-Dawley (SD) rats in conscious state with a computerized blood pressure monitoring system. Four weeks later, the rats were administered with Vitamin D3, and fed with the high-cholesterol diet for 8 weeks to induce atherosclerosis. The hearts and aortae were removed for pathological examination. A negative correlation was found between BRS and the scores of coronary (r=-0.464, P
- Published
- 2005
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