1. Pectic polysaccharide from Nelumbo nucifera leaves promotes intestinal antioxidant defense in vitro and in vivo .
- Author
-
Huang C, Peng X, Pang DJ, Li J, Paulsen BS, Rise F, Chen YL, Chen ZL, Jia RY, Li LX, Song X, Feng B, Yin ZQ, and Zou YF
- Subjects
- Animals, Antioxidants chemistry, Cell Line, Cell Survival drug effects, Epithelial Cells drug effects, Gene Expression Regulation drug effects, Malondialdehyde, NF-E2-Related Factor 2 genetics, NF-E2-Related Factor 2 metabolism, Pectins chemistry, Superoxide Dismutase, Swine, Antioxidants pharmacology, Nelumbo chemistry, Pectins pharmacology, Plant Leaves chemistry
- Abstract
In this study, the Nelumbo nucifera leaf polysaccharide (NNLP) was isolated by hot water extraction and ethanol precipitation. DEAE anion exchange chromatography and gel filtration were further performed to obtained the purified fraction NNLP-I-I, the molecular weight of which was 16.4 kDa. The monosaccharide composition analysis and linkage units determination showed that the fraction NNLP-I-I was a pectic polysaccharide. In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains. The biological studies demonstrated that NNLP-I-I displayed antioxidant properties through mediating the Nrf2-regulated intestinal cellular antioxidant defense, which could protect cultured intestinal cells from oxidative stress and improve the intestinal function of aged mice.
- Published
- 2021
- Full Text
- View/download PDF