1. Pressure-induced changes on the morphology and gene expression in mammalian cells
- Author
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Masanobu Horie, Hideaki Fujita, Masayoshi Nishiyama, Tomonobu M. Watanabe, Yoshie Harada, and Kazuko Okamoto
- Subjects
0301 basic medicine ,QH301-705.5 ,Cellular differentiation ,Science ,Hydrostatic pressure ,Biophysics ,Gene Expression ,Biology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Mechanobiology ,Mice ,0302 clinical medicine ,SOX2 ,Downregulation and upregulation ,Gene expression ,Hydrostatic Pressure ,Animals ,Biology (General) ,Gene ,SOXB1 Transcription Factors ,Cell Differentiation ,Fibroblasts ,mechanobiology ,embryonic stem cells ,pluripotency ,Embryonic stem cell ,Cell biology ,mouse embryonic fibroblast ,030104 developmental biology ,General Agricultural and Biological Sciences ,Octamer Transcription Factor-3 ,030217 neurology & neurosurgery ,Research Article - Abstract
We evaluated the effect of high hydrostatic pressure on mouse embryonic fibroblasts (MEFs) and mouse embryonic stem (ES) cells. Hydrostatic pressures of 15, 30, 60, and 90 MPa were applied for 10 min, and changes in gene expression were evaluated. Among genes related to mechanical stimuli, death-associated protein 3 was upregulated in MEF subjected to 90 MPa pressure; however, other genes known to be upregulated by mechanical stimuli did not change significantly. Genes related to cell differentiation did not show a large change in expression. On the other hand, genes related to pluripotency, such as Oct4 and Sox2, showed a twofold increase in expression upon application of 60 MPa hydrostatic pressure for 10 min. Although these changes did not persist after overnight culture, cells that were pressurized to 15 MPa showed an increase in pluripotency genes after overnight culture. When mouse ES cells were pressurized, they also showed an increase in the expression of pluripotency genes. These results show that hydrostatic pressure activates pluripotency genes in mammalian cells. This article has an associated First Person interview with the first author of the paper., Summary: Application of high hydrostatic pressure on somatic cells induce changes in gene expression including upregulation in pluripotency genes.
- Published
- 2021