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Communicating one's mindset means transmitting complex relationships between concepts and emotions. Using cognitive network science, we reconstruct the mindset around suicide as communicated in 139 genuine suicide notes. Despite their negative context, suicide notes are surprisingly positively valenced and their ending statements are markedly more emotional, i.e. elicit deeper fear/sadness but also stronger joy/trust and anticipation, than their main body. By using emotional states from the Emotional Recall Task, we "open the lid" of suicidal narratives and compare their emotional backbone against emotion recall in mentally healthy individuals. Supported by psychological literature, we introduce emotional complexity as an affective analogue of structural balance theory, measuring how elementary cycles (closed triads) of emotion co-occurrences mix positive, negative and neutral states in narratives and recollections. Both authors of suicide notes and healthy individuals exhibit less complexity and more emotional coherence than expected by chance. However, suicide narratives display higher complexity, i.e. a lower level of coherently valenced triads, than healthy individuals recalling the same states. Entropy measures identified a similar tendency for suicide letters to shift more frequently between contrasting emotional states. Our results demonstrate that suicide notes possess highly contrastive narratives of emotions, more complex than expected by null models and healthy populations.

ObjectiveTo examine the impact of central nervous system (CNS) stimulants on the growth of children with attention-deficit/hyperactivity disorder (ADHD), and to assess the efficacy and feasibility of weight recovery interventions on growth.MethodA total of 230 children aged 5 to 12 years with ADHD with no history of chronic CNS stimulant use were randomly assigned to receive daily CNS stimulants (78%, primarily osmotic release oral system-methylphenidate [OROS-MPH]) or behavioral treatment (22%) for 30 months. After 6 months, children evidencing a decline in body mass index (BMI) of >0.5 z-units were randomized to 1 of 3 weight recovery treatments (WRTs): monthly monitoring of height/weight (MON) plus continued daily medication; drug holidays (DH) with medication limited to school days; or daily caloric supplementation (CS) with a 150-kcal supplement plus daily medication.ResultsBefore WRT assignment, medication was associated with significant reductions in standardized weight and height (p values&nbsp

Inflammation has been implicated in the pathogenesis and maintenance of depressive symptoms. The role of inflammation in depressive symptomatology may be complex, varying within endophenotypes and across the lifespan. Aging is associated with myriad changes in the structure and function of the brain. Yet, little attention has been given to the role of inflammation in depressive symptoms within a lifespan developmental framework. In this study, we examined whether the association between inflammation and depressive symptom domains varied by age. Participants were a community sample of individuals (N= 2,077, Range = 30–84) who participated in the Biomarker projects of the MIDUS2, MIDUS Refresher, or the MIDJA study. Inflammation was indexed by two inflammatory markers consistently implicated in depressed individuals, interleukin 6 (IL-6) and C-reactive protein (CRP), measured in blood. Depressive symptom domains, including depressed affect, anhedonia, somatic complaints, and interpersonal problems, were reportedviathe Center for Epidemiologic Studies—Depression Scale (CES-D). Inflammatory markers were associated with more somatic complaints, more interpersonal problems, and less anhedonia. Age moderated the relationship between inflammatory markers and two depressive symptom subscales. Specifically, the positive association between inflammation and somatic complaints and the negative association between inflammation and anhedonia increased with age. These observations offer preliminary evidence from a large community sample that aging may be an important context for the role of inflammatory signaling in different aspects of psychological and behavioral well-being.

// Xinhua Chen 1, * , Yiling Chen 2, * , Jianwen Jiang 1 , Liming Wu 1 , Shengyong Yin 1 , Xudong Miao 3 , Robert J. Swanson 4 and Shusen Zheng 1 1 Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health, Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University, Zhejiang, 310003, China 2 The Department of Anatomy, Tianjin Medical University, Tianjin, 300070, China 3 The Department of Orthopedics, The Second Affiliated Hospital, Zhejiang University, Zhejiang, 310003, China 4 Anatomical Sciences Department, College of Osteopathic Medicine, Liberty University, Lynchburg, VA, 24515, USA * These authors have contributed equally to this work Correspondence to: Shusen Zheng, email: shusenzheng@zju.edu.cn Xudong Miao, email: mxd5172@163.com Robert J. Swanson, email: rjswanson@liberty.edu Keywords: nano-pulse stimulation (NPS), nanosecond pulsed electric field (nsPEF), non-thermal, ablation, palliative therapy Abbreviations: nsPEF: nanosecond pulsed electric field; ALP: alkaline phosphatase; NPS: nano-pulse stimulation Received: December 16, 2016 Accepted: March 26, 2017 Published: April 18, 2017 ABSTRACT Background: Nanosecond pulsed electric field (nsPEF), which is also termed as nano-pulse stimulation (NPS), has the potential of stimulating immune responses toward cancer cells. The current study investigates its local and systemic antitumor efficacy in vivo in late stage tumors with lung metastasis. Method: The 12 canines with spontaneous osteosarcomas and 12 nude mice transplanted with human hepatocellular carcinoma were divided randomly and were given NPS treatment, surgery or no treatment control. Nanosecond pulsed electric field was delivered with puncture electrodes at 40 kV/cm with 500 pulses at 1 Hz. The survival time, tumor volume, serum alkaline phosphatase (ALP), joint capsule damage and lung metastasis were followed up. The efficacy was compared with control. Results: Nanosecond pulsed electric field reduced primary tumor volume and extended the survival significantly compared to the control group ( P

Many studies have been dedicated to hypertension and hypercholesterolemia, as they are the primary conditions that influence the unfolded protein response (UPR). However, the concurrent effects of these two factors are unknown. Our research used spontaneously hypertensive rats (SHR) fed a cholesterol enriched diet (CED) as model of atherosclerosis formation to discover what effect the simultaneous actions of hypertension and hypercholesterolemia have on the UPR. The combination of hypertension and consumption of a CED (not the CED alone) caused the formation of early atherosclerotic features. Both increased expression of the CCAAT-enhancer-binding protein (CHOP) and the insulin induced gene 1 (INSIG1), which is the target gene of the sterol regulatory element-binding protein 1-c (SREBP1-c), and decreased expression of the spliced x-box binding protein1 (sXBP1) mRNA were observed in the SHR fed a CED. Cholesterol overload strongly suppressed glucose regulated protein 78 (GRP78), glucose regulated protein 94 (GRP 94), and the expression of CHOP and INSIG1 mRNA in both normotensive and hypertensive rats. Unlike other UPR factors, the sXBP1 mRNA expression was strongly downregulated in SHR fed a normal diet but upregulated in those fed a CED. The changes to UPR in the SHR fed a CED were associated with improvement of the initially impaired heart function of the rats.

Horses are stabled overnight for a number of practical reasons; however, there is little research quantifying nocturnal equine behavioral patterns or the extent to which different environments influence nocturnal behavior. The aim of this study was to establish whether differences in duration of sleep and ingestive behaviors were apparent for horses bedded on straw (group 1) or shavings (group 2). Ten geldings of mixed breed (mean age: 7.3 ± 3.53 years) bedded on either shavings (n = 5) or straw (n = 5) were observed between 7 pm and 7 am. Duration of behaviors according to a predefined ethogram was recorded in minutes using a video recorder and continuous focal sampling. Mann–Whitney U tests were used to identify whether any significant differences in duration of ingestion and sleep behaviors occurred for horses bedded on straw compared with shavings. Of the total observation period, group 1 spent, on average, 29.3% of their time budget engaged in recumbent behaviors, compared with 12.2% for group 2. However, no significant differences in duration were established between horses bedded on straw or shavings for standing sleep, sternal recumbency, and lateral recumbency behaviors ( P > 0.05). Ingestive behaviors occupied approximately one-third of the time budget, with no significant difference ( P > 0.05) observed between groups. On average, group 1 spent a longer proportion of the observation period ingesting bedding (8.1%) compared with group 2 (1%). Duration of bedding ingestion appeared to peak between 1 am and 7 am for both groups. Although not quantified, general observations revealed horses were motivated to alternate between eating hay and bedding in both groups, owing to the prevalence of bedding ingestion. The results indicate that straw bedding facilitates the display of ingestive and sleep behaviors, whereas horses bedded on shavings spent a greater proportion of their nocturnal time budget engaged in "other" behaviors. Further research is required to investigate the extent to which different types of bedding material enrich the environment of horses that are stabled overnight.

A recent study has shown that nanosecond pulsed electric fields (nsPEF) can affect the intracellular structures of melanoma within weeks. nsPEF is a non-drug, non-thermal treatment using ultrashort, intense pulsed electric fields with nanosecond durations. In the current study we followed up melanoma histopathology and metastasis with tissue micro-array 5 months post-nsPEF. After nsPEF treatment, tumor growth, tumor histology, metastasis, peri-tumor vessel and micro-vessel density were examined for the effect of nsPEF treatment on melanoma in vivo. The 17 nsPEF-treated mice were tumor-free for 169 days, significantly longer than those 19 control mice bearing melanoma without nsPEF. Histopathology follow-up showed that melanoma did not recur to the primary injection place after complete elimination. Compared with the control tumor, nsPEF-treated tumors present decreased micro-vessel density in a time-course manner in this survival study. Treatment with nsPEF caused continuous histopathological changes in melanomas, eliminated melanoma without recurrence at the primary site and prolonged animal survival time by inhibiting tumor blood supply and leading to tumor infarction. Thus, nsPEF could be applied in a non-ionizing therapeutic approach, without other agents, to locally treat tumors within a defined boundary.

Context:Hip and pelvic injuries are often associated with direct trauma, but spe-cific fractures to the acetabulum are rare. The signs and symptoms of an acetabular fracture can mimic those of conditions that are more common at the hip area, and therefore the specificity of the diagnostic testing is crucial.Objective:To present the case of a female Division I college field-hockey player who developed a superomedial acetabular fracture.Background:The athlete’s initial complaint of intolerable hip pain decreased after a 3-week rest period but persisted with passive internal and external hip rotation. Additional diagnostic testing was needed to differentiate the various pathologies that were associated with her symptoms.Conclusions:Clinicians should be aware of the potential differential diagnoses of the hip and should investigate all potential possibilities even though they might not coincide with the initial injury.

We have discovered a new, ultrafast therapy for treating skin cancer that is extremely effective with a total electric field exposure time of only 180 microsec. The application of 300 high-voltage (40 kV/cm), ultrashort (300 nsec) electrical pulses to murine melanomas in vivo triggers both necrosis and apoptosis, resulting in complete tumor remission within an average of 47 days in the 17 animals treated. None of these melanomas recurred during a 4-month period after the initial melanoma had disappeared. These pulses generate small, long-lasting, rectifying nanopores in the plasma membrane of exposed cells, resulting in increased membrane permeability to small molecules and ions, as well as an increase in intracellular Ca(2+), DNA fragmentation, disruption of the tumor's blood supply and the initiation of apoptosis. Apoptosis was indicated by a 3-fold increase in Bad labeling and a 72% decrease in Bcl-2 labeling. In addition, microvessel density within the treated tumors fell by 93%. This new therapy utilizing nanosecond pulsed electric fields has the advantages of highly localized targeting of tumor cells and a total exposure time of only 180 microsec. These pulses penetrate into the interior of every tumor cell and initiate DNA fragmentation and apoptosis while at the same time reducing blood flow to the tumor. This new physical tumor therapy is drug free, highly localized, uses low energy, has no significant side effects and results in very little scarring.

Objective To investigate the effect of different training modalities on various markers of semen quality. Design Crossover study. Setting Medical school. Patient(s) Forty-five men participated voluntarily in the study, being allocated into three groups according to their sports practice. Intervention(s) None. Main Outcome Measure(s) Sperm parameters (volume, liquefaction time, pH, viscosity, sperm count, motility, and morphology). Result(s) Sperm concentration; total sperm number; type "a," "b," and "d" velocity; and morphology were significantly different among the practitioners of the three different training modalities. Morphology was the parameter showing the greatest difference, even reaching clinical relevance for the triathlete group (4.7%, poor prognosis pattern). In addition, these parameters tended to decrease as training requirements increased. Conclusion(s) There are differences in the seminal profiles of individuals exercising in different modalities. The differences are more marked as intensity and volume of exercise increase, especially for morphology. These variables ought to be carefully analyzed and taken into account when designing a training protocol, especially with higher-level athletes, so that reproductive function is not compromised.

A prospective study was planned to evaluate sperm morphology as a parameter to predict the fertilization outcome in an in vitro fertilization program. Couples applying to in vitro fertilization were admitted into this project when the sperm concentration was greater than 20 million per mL and motility greater than 30 per cent. Based on new strict criteria for evaluating normal sperm morphology, patients were divided prospectively into 2 groups. In group I (25 patients) normal sperm morphology was less than 14 per cent, and in group II (71 patients) normal sperm morphology was greater than 14 per cent, using a threshold established previously. Multiple regression analysis was used to evaluate different parameters: concentration, motility, and morphology against the dependent variables, fertilization, and cleavage. The only factor which was significantly correlated with fertilization and cleavage was normal sperm morphology (P less than 0.0001). The fertilization rate (per oocyte) and the cleavage rate were 49.4 per cent and 47.6 per cent in group I and 88.3 per cent and 87 per cent in group II (P less than 0.0001). The ongoing pregnancy rate per laparoscopy and per embryo transfer was 4 per cent and 5.5 per cent, respectively, in group I and 18.3 per cent and 18.5 per cent, respectively, in group II (no significant difference). This study demonstrates the value of analyzing sperm morphology using the criteria recommended in terms of predicting fertilization and perhaps pregnancy outcome. Patients can be better counseled and the probability of fertilization or no fertilization can be more accurately established. Furthermore a trend is shown in the pregnancy rate that may indicate the importance of the male genome in establishing a pregnancy.

In vitro fertilization (IVF) followed by embryo transfer (replacement) to the patient donating the female gametes and using the husband’s sperm for fertilization purposes is the classic form of therapy, devised in the 197Os, to overcome the problem of female infertility due to irreparable tubal damage.’ As experience increased with the procedure, several other indications were found for this therapeutic modality, namely, in the female the presence of endometriosis which does not respond to the commonly used treatment protocols, severe cervical factors that cannot be overcome by intrauterine insemination, the presence of systemic or local antisperm antibodies as the only detectable factor for infertility, and the female who has no identifiable factors for her reproductive problem and who has a normal husband, but who nevertheless cannot achieve pregnancy (infertile normal couple). Soon it was realized that IVF needed a small amount of sperm (as low as perhaps 12,000) for fertilization to occur. Therefore, it was immediately inferred that this procedure may also be indicated in infertility due to male factor. Several groups started working with this type of patient, trying to correct the fertility problem in males in whom the commonly accepted treatment protocols had already failed. Other forms of assisted reproduction have been developed as variants of the classic type of IVF with embryo replacement (gamete intrafallopian transfer, gamete donation from male

Hepatocellular carcinoma (HCC) is a highly aggressive malignancy. Nanosecond pulsed electric field (nsPEF) is a new technology destroying tumor cells with a non-thermal high voltage electric field using ultra-short pulses. The study’s aim was to evaluate the ablation efficacy of nsPEFs with human HCC cell lines and a highly metastatic potential HCC xenograft model on BALB/c nude mice. The in vivo study showed nsPEFs induced HCC cell death in a dose dependent manner. On the high metastatic hepatocellular carcinoma cell line (HCCLM3) xenograft mice model, tumor growth was inhibited significantly in nsPEF-treated- groups (single dose and multi-fractionated dose). Besides a local effect, the nsPEF treatment reduced pulmonary metastases. The nsPEFs also enhanced HCC cell phagocytosis by human macrophage cell (THP1) in vitro. The nsPEF is efficient in controlling HCC progression and reducing its metastasis. NsPEF treatment may elicit a host immune response against tumor cells. This study suggests nsPEF therapy could be used as a potential locoregional therapy for hepatocellular carcinoma.

Fresh semen specimens from 46 patients and donors were evaluated for concentration, motility, velocity, and linearity using two different commercially available computerized sperm motion analyzer systems. Although no significant differences in measurement of concentration or motility were observed, significant differences in velocity and linearity were recorded. Fourteen cryopreserved/thawed samples were assessed with the same set-up parameters as fresh specimens. When discrepancies between manual and computer counts were noted, the authors changed the set-up parameters and evaluated 33 additional specimens. Again, no differences in concentration and motility, but significant differences in velocity and linearity were observed. Interlaboratory results must be correlated and standardization of set-up parameters of various analyzers is essential.