1. NMR-based metabolic profiling and discrimination of wild tropical tunas by species, size category, geographic origin, and on-board storage condition
- Author
-
Fany Sardenne, Aurélien Amiel, Hervé Guillou, Emmanuel Chassot, Marie Tremblay-Franco, Nathalie Bodin, Edwin Fouché, Cécile Canlet, Laurent Debrauwer, Institut de Recherche pour le Développement (IRD), Analyse de Xénobiotiques, Identification, Métabolisme (E20 Metatoul-AXIOM), MetaToul-MetaboHUB, Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-ToxAlim (ToxAlim), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ToxAlim (ToxAlim), Prévention et promotion de la cancérogénèse par les aliments (ToxAlim-PPCA), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Toxicologie Intégrative & Métabolisme (ToxAlim-TIM), This work is a contribution to the project 'CANAL: Influence of environmental and biological factors on tuna meat quality' with the financial support of Thai Union Europe Group (ex-MWBrands Ltd) . All NMR experiments were performed on the instruments of the MetaToul-AXIOM platform, partner of the national infrastructure of metabolomics and fluxomics: MetaboHUB (MetaboHUB-ANR-11-INBS-0010, 2011), ANR-11-INBS-0010,METABOHUB,Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation(2011), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), and Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse)
- Subjects
Skipjack tuna ,Yellowfin tuna ,Magnetic Resonance Spectroscopy ,[SDV]Life Sciences [q-bio] ,Anserine ,Carnosine ,Bigeye tuna ,Purse seine tuna fisheries ,Fish measurement ,Analytical Chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Animals ,Metabolomics ,High-resolution 1H NMR ,14. Life underwater ,Food science ,Indian Ocean ,030304 developmental biology ,0303 health sciences ,biology ,Tuna ,Fishes ,food and beverages ,04 agricultural and veterinary sciences ,General Medicine ,biology.organism_classification ,Highly migratory large pelagic fish ,040401 food science ,chemistry ,Raw white and red muscles ,Western Indian Ocean ,human activities ,Thunnus ,Food Science - Abstract
International audience; Tunas are among the most traded and valued fish species, and good traceability of tuna products in the world market is needed to protect both consumers and tuna stocks. To that purpose, high-resolution proton nuclear magnetic resonance (1H NMR) spectroscopy combined with multivariate data analysis was used to investigate the molecular components of the aqueous extract of white and red muscles in three species of wild tropical tuna species, namely yellowfin tuna (Thunnus albacares), skipjack tuna (Katsuwonus pelamis) and bigeye tuna (T. obesus). Principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) applied to the processed 1H NMR spectra showed significant separation according to the species and size category (i.e., small tunas < 80 cm fork length vs large tunas > 80 cm fork length), the storage conditions on-board the purse-seine vessels (i.e., brine- vs deep-freezing), and the geographical origin (i.e., where the tuna was caught: Mozambique Channel vs western-central Indian Ocean). The major groups of metabolites responsible for differentiation in PLS-DA score plots were the dipeptides (anserine, carnosine) and organic acids (lactate, creatine/phosphocreatine) in the white muscle, and the free amino acids, essential nutrients (choline and its derivatives, phosphatidylethanolamine), dipeptides and organic acids in the red muscle. Our results showed that NMR-based metabolomics is a powerful tool to efficiently discriminate specific profiles among wild tuna species, raw muscle tissues, fish storage conditions and tuna geographical origin.
- Published
- 2022