1. Intracellular β2-adrenergic receptor signaling specificity in mouse skeletal muscle in response to single-dose β2-agonist clenbuterol treatment and acute exercise
- Author
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Ryosuke Mitsuhashi, Kazuhiko Imaizumi, Hideki Ohno, Ken Shirato, Shogo Sato, Kaoru Tachiyashiki, Takako Kizaki, and Daisuke Inoue
- Subjects
Male ,MAPK/ERK pathway ,β2-Agonist clenbuterol ,medicine.medical_specialty ,Physiology ,Short Communication ,p38 mitogen-activated protein kinases ,Skeletal muscle ,p38 MAPK ,p38 Mitogen-Activated Protein Kinases ,Mice ,β2-Adrenergic receptor signaling ,Physical Conditioning, Animal ,Internal medicine ,medicine ,Animals ,Clenbuterol ,Phosphorylation ,Muscle, Skeletal ,Protein kinase A ,Exercise ,Adrenergic beta-2 Receptor Agonists ,Protein kinase B ,Soleus muscle ,business.industry ,Akt ,musculoskeletal system ,Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha ,Mice, Inbred C57BL ,Muscle Fibers, Slow-Twitch ,Endocrinology ,medicine.anatomical_structure ,Muscle Fibers, Fast-Twitch ,business ,Proto-Oncogene Proteins c-akt ,Transcription Factors ,medicine.drug - Abstract
The aim of this study was to clarify the intracellular β2-adrenergic receptor signaling specificity in mouse slow-twitch soleus and fast-twitch tibialis anterior (TA) muscles, resulting from single-dose β2-agonist clenbuterol treatment and acute exercise. At 1, 4, and 24 h after single-dose treatment with clenbuterol or after acute running exercise, the soleus and TA muscles were isolated and subjected to analysis. The phosphorylation of p38 mitogen-activated protein kinase (MAPK) increased after single-dose clenbuterol treatment and acute exercise in the soleus muscle but not in the TA muscle. Although there was no change in the phosphorylation of Akt after acute exercise in either muscle, phosphorylation of Akt in the soleus muscle increased after single-dose clenbuterol treatment, whereas that in the TA muscle remained unchanged. These results suggest that p38 MAPK and Akt pathways play a functional role in the adaptation to clenbuterol treatment and exercise, particularly in slow-twitch muscles.
- Published
- 2013