1. Precisely Encoded Barcodes through the Structure‐Fluorescence Combinational Strategy: A Flexible, Robust, and Versatile Multiplexed Biodetection Platform with Ultrahigh Encoding Capacities
- Author
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Jingwei Yi, Qingsheng Guo, Xiaoxia Fang, Yimei Cao, Jiancong Wu, Yao Wang, Jing He, Xiaomei Chi, Chongzhao Wu, Hong Xu, Hongchen Gu, Cang Chen, Hajar Masoomi, and Jian Ye
- Subjects
Materials science ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,Barcode ,01 natural sciences ,Multiplexing ,law.invention ,Biomaterials ,Suspension array technology ,law ,Encoding (memory) ,Quantum Dots ,General Materials Science ,Electronic Data Processing ,General Chemistry ,Flow Cytometry ,021001 nanoscience & nanotechnology ,Single excitation ,Fluorescence ,Microspheres ,0104 chemical sciences ,Quantum dot ,Nanoparticles ,Magnetic nanoparticles ,0210 nano-technology ,Biotechnology - Abstract
With the rapid development of suspension array technology, microbeads-based barcodes as the core element with sufficient encoding capacity are urgently required for high-throughput multiplexed detection. Here, a novel structure-fluorescence combinational encoding strategy is proposed for the first time to establish a barcode library with ultrahigh encoding capacities. Based on the never revealed transformability of the structural parameters (e.g., porosity and matrix component) of mesoporous microbeads into scattering signals in flow cytometry, the enlargement of codes number has been successfully realized in combination with two other fluorescent elements of fluorescein isothiocyanate isomer I (FITC) and quantum dots (QDs). The barcodes are constructed with precise architectures including FITC encapsulated within mesopores and magnetic nanoparticles as well as QDs immobilized on the outer surface to achieve the ultrahigh encoding level of 300 accompanied with superparamagnetism. To the best of knowledge, it is the highest record of single excitation laser-based encoding capacity up to now. Moreover, a ten-plexed tumor markers bioassay based on the tailored-designed barcodes has been evaluated to confirm their feasibility and effectiveness, and the results indicate that the barcodes platform is a promising and robust tool for practical multiplexed biodetection.
- Published
- 2021