1. Proteins involved in the endoplasmic reticulum stress are modulated in synovitis of osteoarthritis, chronic pyrophosphate arthropathy and rheumatoid arthritis, and correlate with the histological inflammatory score
- Author
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Gaël Cobraiville, Philippe Delvenne, Dominique de Seny, Elettra Bianchi, Marie-Joëlle Kaiser, Michel Malaise, Gabriel Mazzucchelli, Jean-Philippe Hauzeur, Dominique Baiwir, Charlotte Collin, and Mégane Deliège
- Subjects
Adult ,Male ,0301 basic medicine ,Pathology ,medicine.medical_specialty ,Proteome ,lcsh:Medicine ,Chondrocalcinosis ,Osteoarthritis ,Article ,S100A9 ,S100A8 ,Arthritis, Rheumatoid ,03 medical and health sciences ,Rheumatic diseases ,0302 clinical medicine ,Rheumatology ,Synovitis ,Arthropathy ,medicine ,Humans ,lcsh:Science ,Endoplasmic Reticulum Chaperone BiP ,Aged ,Retrospective Studies ,Aged, 80 and over ,Inflammation ,Multidisciplinary ,business.industry ,lcsh:R ,Proteins ,Middle Aged ,Endoplasmic Reticulum Stress ,medicine.disease ,HSPA1A ,Diphosphates ,030104 developmental biology ,medicine.anatomical_structure ,Rheumatoid arthritis ,Female ,lcsh:Q ,Inflammation Mediators ,Synovial membrane ,business ,Biomarkers ,030217 neurology & neurosurgery - Abstract
It is now well recognized that osteoarthritis (OA) synovial membrane presents inflammatory components. The aim of this work is to provide evidence that similar inflammatory mechanisms exist in synovial membrane (n = 24) obtained from three pathologies presenting altogether an inflammatory gradient: OA, chronic pyrophosphate arthropathy (CPPA) and rheumatoid arthritis (RA). Synovial biopsies were first characterized by a histological score based on synovial hyperplasia and infiltration of lymphocytes, plasma cells, polymorphonuclear and macrophages. All biopsies were also analyzed by 2D-nano-UPLC-ESI-Q-Orbitrap for protein identification and quantification. Protein levels were correlated with the histological score. Histological score was in the range of 3 to 8 for OA, 5 to 13 for CPPA and 12 to 17 for RA. Of the 4,336 proteins identified by mass spectrometry, 51 proteins were selected for their strong correlation (p DNAJB11, CALR, ERP29, GANAB, HSP90B1, HSPA1A, HSPA5, HYOU1, LMAN1, PDIA4, and TXNDC5) were involved in the endoplasmic reticulum (ER) stress. Protein levels of S100A8 and S100A9 were significantly higher in RA compared to OA (for both) or to CPPA (for S100A8 only) and also significantly correlated with the histological score. Eighteen complement component proteins were identified, but only C1QB and C1QBP were weakly correlated with the histological score. This study highlights the inflammatory gradient existing between OA, CPPA and RA synovitis either at the protein level or at the histological level. Inflamed synovitis was characterized by the overexpression of ER stress proteins.
- Published
- 2020