1. An ic-ELISA and immunochromatographic strip assay for the detection of 2,4-dichlorophenoxyacetic acid in bean sprouts and cabbage.
- Author
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Pan X, Xu X, Song S, Xu L, Kuang H, Wu X, Liu L, and Xu C
- Subjects
- Antibodies, Monoclonal, Chromatography, Affinity, Limit of Detection, 2,4-Dichlorophenoxyacetic Acid analysis, Brassica chemistry, Enzyme-Linked Immunosorbent Assay, Immunoassay, Phaseolus chemistry
- Abstract
The compound 2,4-dichlorophenoxyacetic acid (2,4-D) is a synthetic plant growth regulator, which is widely used in agricultural production. Consequently, it is necessary to establish a rapid and sensitive detection method to monitor its use and prevent the environmental and human health problems caused by overuse. In this study, a monoclonal antibody (mAb) 2D5 against 2,4-D was prepared, and based on it, an ic-ELISA and an immunochromatographic strip assay (ICA) were developed for rapid detection. The 50% inhibitory concentration (IC
50 ) of the mAb against 2,4-D was 1.7 ng mL-1 and the mAb showed no cross-reactivity with other plant growth regulators with similar structures to 2,4-D. Under optimum conditions, the ICA showed a visual detection limit (vLOD) of 20 ng mL-1 and a cut-off value of 200 ng mL-1 in bean sprouts, and cabbage samples gave the same results. The quantitative detection showed the linear detection ranges for bean sprouts and cabbage samples were 6.3-73.0 and 5.4-132.6 ng mL-1 , respectively. The ICA was successfully applied to detect 2,4-D in bean sprouts and cabbage samples and achieved good recoveries ranging from 94.7% to 104.3% with a coefficient of variation (CV) less than 8.3%. Hence, the ICA provides a sensitive, efficient and reliable detection method, which has broad application prospects in the detection of a large number of samples., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)- Published
- 2022
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