1. Ultrasensitive immunosensor for prostate specific antigen using biomimetic polydopamine nanospheres as an electrochemiluminescence superquencher and antibody carriers.
- Author
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Liu Y, Zhao Y, Zhu Z, Xing Z, Ma H, and Wei Q
- Subjects
- Fluorocarbon Polymers chemistry, Humans, Luminescent Measurements, Nanotubes, Carbon chemistry, Organometallic Compounds chemistry, Prostate-Specific Antigen blood, Antibodies, Immobilized chemistry, Biomimetic Materials chemistry, Immunoassay methods, Indoles chemistry, Limit of Detection, Nanospheres chemistry, Polymers chemistry, Prostate-Specific Antigen analysis
- Abstract
In this work, biomimetic polydopamine nanospheres (PDANSs) were easily prepared and were firstly exploited as an electrochemiluminescence (ECL) nanoquencher for the development of a sandwich type immunosensor. The PDANSs with abundant active functional groups can facilely label the detection antibody and show remarkable quenching effect towards the ECL of the tris-(2,2'-bipyridine)ruthenium (Ru(bpy)
3 2+ ). The amino-modified multiwall carbon nanotubes/Nafion (MWCNTs-NH2 @N) composite-film was adopted as a matrix to incorporate the luminophor Ru(bpy)3 2+ and immobilize the capture antibody. The prominent decrease of ECL signal intensity was obtained on account of the unique quenching ability of the labeled PDANSs. The quenching mechanism is believed that the excited states of Ru(bpy)3 2+ can be annihilated by quinone units in PDANSs via energy transfer. The ECL quenching efficiency was logarithmically related to the concentration of the prostate specific antigen (PSA) in the range from 0.1 pg mL-1 to 20 ng mL-1 with a detection limit of 35 fg mL-1 . Furthermore, the proposed ECL immunosensor presented good stability, repeatability and selectivity., (Copyright © 2017 Elsevier B.V. All rights reserved.)- Published
- 2017
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