41 results on '"Yoshiro Suzuki"'
Search Results
2. Blood pressure after treatment with sodium–glucose cotransporter 2 inhibitors influences renal composite outcome: Analysis using propensity score‐matched models
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Hareaki Yamamoto, Akira Kanamori, Daisuke Suzuki, Masao Toyoda, Nobuo Hatori, Hisakazu Degawa, Kazuyoshi Sato, Takayuki Furuki, Hiroyuki Sakai, Kouta Aoyama, Kouichi Tamura, Keiichi Chin, Masahiro Takihata, Shun Ito, Fuyuki Minagawa, Masahiro Hayashi, Toshimasa Hishiki, Kohsuke Minamisawa, Yoshiro Suzuki, Hiroshi Takeda, Togo Aoyama, Hideo Machimura, Yutaka Hatori, Shinichi Umezawa, Masaaki Miyakawa, Yoshiro Hamada, Tomoya Umezono, and Kazuo Kobayashi
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Blood Glucose ,Male ,medicine.medical_specialty ,Mean arterial pressure ,Diabetic Cardiomyopathies ,Endocrinology, Diabetes and Metabolism ,Urology ,Renal function ,Blood Pressure ,Diabetic nephropathy ,030204 cardiovascular system & hematology ,03 medical and health sciences ,0302 clinical medicine ,Diabetes mellitus ,Internal Medicine ,medicine ,Humans ,Diabetic Nephropathies ,030212 general & internal medicine ,Renal Insufficiency, Chronic ,Propensity Score ,Sodium-Glucose Transporter 2 Inhibitors ,Retrospective Studies ,Glycated Hemoglobin ,business.industry ,Articles ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,Clinical Science and Care ,Blood pressure ,Diabetes Mellitus, Type 2 ,Sodium–glucose cotransporter 2 Inhibitors ,Propensity score matching ,Albuminuria ,Original Article ,Female ,medicine.symptom ,business ,Biomarkers ,Follow-Up Studies ,Glomerular Filtration Rate ,Kidney disease - Abstract
Aims/Introduction Sodium–glucose cotransporter 2 inhibitors (SGLT2i) improve renal outcome in patients with type 2 diabetes mellitus, but the mechanism is not fully understood. The aim of this retrospective study was to assess the association of achieved blood pressure with renal outcomes in Japanese type 2 diabetes mellitus patients with chronic kidney disease. Materials and Methods We assessed 624 Japanese type 2 diabetes mellitus patients with chronic kidney disease taking SGLT2i for >1 year. The patients were classified as those with post‐treatment mean arterial pressure (MAP) of ≥92 mmHg (n = 344) and those with MAP of, Incidence of renal composite outcome and changes in logarithmic value of the albumin‐to‐creatinine ratio and estimated glomerular filtration rate.
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- 2020
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3. TRPM8 channel is involved in the ventilatory response to CO2 mediating hypercapnic Ca2+ responses
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Makoto Tominaga, Yoshiro Suzuki, Yoshitaka Oku, and Yutaka Hirata
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Pulmonary and Respiratory Medicine ,Genetically modified mouse ,medicine.medical_specialty ,Physiology ,Chemistry ,General Neuroscience ,03 medical and health sciences ,Transient receptor potential channel ,0302 clinical medicine ,Endocrinology ,030228 respiratory system ,Internal medicine ,Knockout mouse ,Respiration ,TRPM8 ,medicine ,medicine.symptom ,Respiratory system ,Hypercapnia ,030217 neurology & neurosurgery ,Intracellular - Abstract
The role of TRP channels in the ventilatory response to CO2 was investigated in vivo. To this end, the respiration of unrestrained adult TRPM8-, TRPV1- and TRPV4-channel knockout mice was measured using whole-body plethysmography. Under control conditions and hyperoxic hypercapnia, no difference in respiratory parameters was observed between adult wild-type mice and TRPV1- and TRPV4-channel knockout mice. However, TRPM8-channel knockout mice showed decreased tidal volume under both hypercapnia and resting conditions. In addition, the expression of TRPM8, TRPV1 and TRPV4 mRNAs was detected in EGFP-positive glial cells in the medulla of GFAP promoter-EGFP transgenic mice by real-time PCR. Furthermore, we measured intracellular Ca2+ responses of TRPM8-overexpressing HEK-293 cells to hypercapnic acidosis. Subpopulations of cells that exhibited hypercapnic acidosis-induced Ca2+ response also responded to the application of menthol. These results suggest that TRPM8 partially mediates the ventilatory response to CO2 via changes in intracellular Ca2+ and is a chemosensing protein that may be involved in detecting endogenous CO2 production.
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- 2019
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4. TRPV6 Gene Mutation in a Dizygous Twin With Transient Neonatal Hyperparathyroidism
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Sumie Yamashita, Hiroshi Mizumoto, Hirotake Sawada, Yoshiro Suzuki, and Daisuke Hata
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0301 basic medicine ,medicine.medical_specialty ,TRPV6 ,Parathyroid, Bone, and Mineral Metabolism ,Endocrinology, Diabetes and Metabolism ,030209 endocrinology & metabolism ,Case Report ,medicine.disease_cause ,Compound heterozygosity ,vitamin D deficiency ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,maternal–fetal calcium transport ,TRPV6 Gene ,transient neonatal hyperparathyroidism ,Mutation ,Fetus ,business.industry ,medicine.disease ,030104 developmental biology ,Endocrinology ,Transient Neonatal Hyperparathyroidism ,Secondary hyperparathyroidism ,business - Abstract
Maternal–fetal transport of calcium (Ca2+) is important for bone mineralization in fetal development. Insufficient Ca2+ transport causes transient neonatal hyperparathyroidism (TNHP). Transient receptor potential cation channel, subfamily V, member 6 (TRPV6), has been found to play an important role in the active transport of Ca2+ through the placenta. Recently, TRPV6 gene was found to be the gene responsible for TNHP with severe skeletal undermineralization. To date, only seven cases of TNHP caused by TRPV6 recessive mutations have been reported. We present a case of TNHP caused by TRPV6 gene mutations. A female newborn was hospitalized because of respiratory distress. Marked undermineralization of the skeleton was observed in X-ray imaging. Laboratory examination revealed markedly high PTH and absence of hypercalcemia along with vitamin D deficiency. Her twin brother presented with almost no symptoms. Maternal laboratory findings indicated normocalcemia, but vitamin D deficiency with a high PTH level for the lactation period was observed. We initially diagnosed the patient as having secondary hyperparathyroidism because of maternal vitamin D deficiency. Nevertheless, the reasons underlying the discordant clinical manifestations between the twin siblings remained unclear. Our analysis of TRPV6 gene clarified that the patient had compound heterozygote mutations, which were reported previously (p.Ile223Thr and p.Gly428Arg). Pathologic mutations in TRPV6 gene were not detected in the other sibling. The clinical symptoms in the patient were transient: they resolved during infancy. TNHP caused by TRPV6 gene mutations is a unique disease in terms of its transient pathology in utero and relief after birth.
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- 2019
5. Expression of the TRPM6 in mouse placental trophoblasts; potential role in maternal–fetal calcium transport
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Claire T. Saito, Makoto Tominaga, Yoshiro Suzuki, and Masaki Watanabe
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0301 basic medicine ,Cell physiology ,medicine.medical_specialty ,Cell Membrane Permeability ,Physiology ,Placenta ,TRPM Cation Channels ,chemistry.chemical_element ,Calcium ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Pregnancy ,TRPM7 ,Internal medicine ,medicine ,Animals ,Humans ,Maternal-Fetal Exchange ,Ion transporter ,Messenger RNA ,Fetus ,Ion Transport ,Chemistry ,HEK 293 cells ,Trophoblasts ,Cell biology ,HEK293 Cells ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,embryonic structures ,Female ,030217 neurology & neurosurgery - Abstract
The placenta is required to transport calcium (Ca2+) from mother to fetus during fetal bone mineralization. In an attempt to clarify the molecular basis of Ca2+ entry for this transport, we identified TRPM6 as a candidate for apical Ca2+ entry pathway. TRPM6 mRNA increased during the last 4 days of pregnancy, coinciding with fetal bone mineralization in mice. TRPM6 mRNA and protein was localized in the trophoblasts in labyrinth where the maternal–fetal Ca2+ transport occurs. In patch-clamp recordings, we observed TRPM6/TRPM7-like currents in mouse trophoblasts after starting fetal bone mineralization but not before mineralization. Plasma membrane Ca2+ permeability was significantly increased in TRPM6/TRPM7 expressed HEK293 cells under physiological Mg2+ and ATP concentration but not in TRPM6 or TRPM7 homomer-expressing cells. These results suggest that TRPM6 is functionally expressed in mouse placental trophoblasts, implicating in maternal–fetal Ca2+ transport likely with TRPM7, which might enable to sustain fetal bone mineralization.
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- 2016
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6. Chemical Inhibitors of the Calcium Entry Channel TRPV6
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Katrin A. Bolanz, Yoshiro Suzuki, Matthias A. Hediger, and Christopher P. Landowski
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Male ,Pyrrolidines ,Miconazole ,Pharmaceutical Science ,chemical inhibitors ,Prostate cancer ,0302 clinical medicine ,Pharmacology (medical) ,RNA, Small Interfering ,Cells, Cultured ,0303 health sciences ,Voltage-dependent calcium channel ,prostate cancer ,Calcium Channel Blockers ,3. Good health ,Cell biology ,030220 oncology & carcinogenesis ,Molecular Medicine ,Female ,Biotechnology ,medicine.medical_specialty ,TRPV6 ,chemistry.chemical_element ,TRPV Cation Channels ,Breast Neoplasms ,Calcium ,03 medical and health sciences ,Inhibitory Concentration 50 ,breast cancer ,SDG 3 - Good Health and Well-being ,Internal medicine ,medicine ,Animals ,Humans ,030304 developmental biology ,Cell Proliferation ,Pharmacology ,Calcium metabolism ,business.industry ,Calcium channel ,Organic Chemistry ,Cancer ,Prostatic Neoplasms ,medicine.disease ,Endocrinology ,chemistry ,Cancer cell ,calcium channel ,Calcium Channels ,Econazole ,business - Abstract
Purpose: Calcium entry channels in the plasma membrane are thought to play a major role in maintaining cellular Ca2+ levels, crucial for growth and survival of normal and cancer cells. The calcium-selective channel TRPV6 is expressed in prostate, breast, and other cancer cells. Its expression coincides with cancer progression, suggesting that it drives cancer cell growth. However, no specific inhibitors for TRPV6 have been identified thus far. Methods: To develop specific TRPV6 inhibitors, we synthesized molecules based on the lead compound TH-1177, reported to inhibit calcium entry channels in prostate cancer cells in vitro and in vivo. Results: We found that one of our compounds (#03) selectively inhibited TRPV6 over five times better than TRPV5, whereas TH-1177 and the other synthesized compounds preferentially inhibited TRPV5. The IC50 value for growth inhibition by blocking endogenous Ca2+ entry channels in the LNCaP human prostate cancer cell line was 0.44 ± 0.07 μM compared to TH-1177 (50 ± 0.4 μM). Conclusions: These results suggest that compound #03 is a relatively selective and potent inhibitor for TRPV6 and that it is an interesting lead compound for the treatment of prostate cancer and other cancers of epithelial origin.
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- 2018
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7. Biphasic Renal Sympathetic Response to Hemorrhagic Hypotension in Mice
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Tao Zhang, Wei Yang, Toshishige Shibamoto, Yasutaka Kurata, Kunitoshi Uchida, Makoto Tominaga, Yoshiro Suzuki, Mamoru Tanida, and Yuhichi Kuda
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0301 basic medicine ,Male ,medicine.medical_specialty ,Sympathetic nervous system ,Sympathetic Nervous System ,medicine.medical_treatment ,TRPV Cation Channels ,Vagotomy ,Critical Care and Intensive Care Medicine ,Inhibitory postsynaptic potential ,Kidney ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Heart Rate ,Internal medicine ,Heart rate ,medicine ,Animals ,business.industry ,Kidney metabolism ,Hemorrhagic hypotension ,Mice, Inbred C57BL ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,Renal physiology ,Emergency Medicine ,Female ,Hypotension ,business ,030217 neurology & neurosurgery - Abstract
The inhibitory responses of renal sympathetic nerve activity (RSNA) and heart rate (HR) to sustained hemorrhagic shock occurred in anesthetized rats, but have not yet been determined in mice. Here, we investigated the responses of RSNA and HR to hemorrhagic hypotension in anesthetized mice, with an emphasis on the molecule-based mechanism for roles of afferent vagal nerves.RSNA, HR, and mean systemic arterial pressure were continuously measured in male pentobarbital-anesthetized C57BL/6N mice. Hemorrhagic hypotension of 50 mmHg was evoked and maintained for 10 min.During hemorrhagic hypotension, RSNA initially increased and then sustainedly decreased, while HR progressively decreased. Vagotomy eliminated the second-phase sympathoinhibition and bradycardia, and carotid sinus denervation with vagotomy abolished the initial renal sympathoexcitation. The renal sympathoinihibition during hemorrhagic hypotension of 50 mmHg was eliminated in mice pretreated with a transient receptor potential vanilloid type 1 channel (TRPV1) inhibitor, capsazepine, and in TRPV1 knockout (TRPV1) mice, but not in TRPV4 knockout mice. The bradycardia response to hemorrhagic hypotension was also absent in TRPV1 mice and mice pretreated with capsazepine.Hemorrhagic hypotension in anesthetized mice causes biphasic responses of RSNA with an initial increase, followed by a sustained decrease, and a progressive decrease in HR. The initial sympathoexcitation is mediated by carotid sinus baroreceptors, while the later sympathoinhibition and bradycardia are mediated via the TRPV1 signals of vagal afferents.
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- 2017
8. Potential role of transient receptor potential (TRP) channels in bladder cancer cells
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Ryohei Hattori, Yoshiro Suzuki, Hideki Mizuno, Masaki Watanabe, Takaaki Sokabe, Momokazu Gotoh, Makoto Tominaga, and Tokunori Yamamoto
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Male ,TRPV4 ,medicine.medical_specialty ,Physiology ,TRPM Cation Channels ,TRPV Cation Channels ,Biology ,Mice ,Transient receptor potential channel ,TRPM7 ,Cell Line, Tumor ,Internal medicine ,medicine ,Animals ,RNA, Messenger ,Cells, Cultured ,Cell Proliferation ,Mice, Inbred C3H ,Bladder cancer ,Cancer ,medicine.disease ,Endocrinology ,Urinary Bladder Neoplasms ,Cell culture ,Cancer cell ,Cancer research ,Calcium ,Calcium Channels ,Urothelium ,Intracellular - Abstract
Transient receptor potential (TRP) channels play important roles in thermal, chemical, and mechanical sensation in various tissues. In this study, we investigated the differences in urothelial TRP channels between normal urothelial cells and bladder cancer cells. TRPV2 and TRPM7 expression levels and TRPV2 activator-induced intracellular Ca(2+) increases were significantly higher, whereas TRPV4 expression and TRPV4 activator-induced intracellular Ca(2+) increases were significantly lower in mouse bladder cancer (MBT-2) cells compared to normal mouse urothelial cells. The proliferation rate of MBT-2 cells overexpressing dominant-negative TRPV2 was significantly increased. In contrast, treatment with TRPV2 activators significantly decreased the proliferation rate. TRPM7-overexpressing MBT-2 cells proliferated more slowly, as compared to mock-transfected cells. Moreover, expression of dominant-negative TRPV2 significantly decreased plasma membrane Ca(2+) permeability of MBT-2 cells as compared to that in mock-transfected cells. Increases in the expression of TRPV2 mRNA, immunoreactivity, and TRPV2 activator-induced intracellular Ca(2+) were also observed in T24 human bladder cancer cells. These results suggested that TRPV2 and TRPM7 were functionally expressed in bladder cancer cells and served as negative regulators of bladder cancer cell proliferation, most likely to prevent excess mechanical stresses.
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- 2014
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9. Blood pressure differences between office and home settings among Japanese normotensive subjects and hypertensive patients
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Shouhei Yuasa, Hareaki Yamamoto, Keiichi Chin, Yoshiro Suzuki, Hisao Mori, Hiroshi Ukai, Takuma Katsumata, and Satoshi Umemura
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Adult ,Male ,medicine.medical_specialty ,Physiology ,Office Visits ,Office visits ,Diastole ,Blood Pressure ,030204 cardiovascular system & hematology ,03 medical and health sciences ,0302 clinical medicine ,Japan ,Internal medicine ,Internal Medicine ,Medicine ,Humans ,In patient ,Statistical analysis ,030212 general & internal medicine ,Morning ,Aged ,business.industry ,Blood Pressure Determination ,Middle Aged ,Blood pressure ,Hypertension ,Cardiology ,Female ,Cardiology and Cardiovascular Medicine ,business ,White Coat Hypertension ,circulatory and respiratory physiology - Abstract
This study attempted to clarify the differences in blood pressure (BP) between the office (clinic) and home settings in patients with controlled, sustained, masked or white-coat hypertension. The following formula was used: office mean systolic BP (omSBP)-mean morning home SBP (mmhSBP)/office mean diastolic BP (omDBP)-mean morning home DBP (mmhDBP). The paired t-test was used for statistical analysis. The omSBP-mmhSBP/omDBP-mmhDBP calculation yielded the following results: among normotensive subjects, -1.1±11.2/-1.7±8.5 mm Hg (mean SBP and mean DBP were higher at home than in the office; n=451, P=0.038 in SBP, P=0.000 in DBP); in controlled hypertensive patients, -0.42±10.9/-2.2±8.2 mm Hg (n=1362, P=0.160 in SBP, P=0.000 in DBP); among sustained hypertensive patients, 5.6±14.7/0.048±9.9 mm Hg (n=1370, P=0.000 in SBP, P=0.857 in DBP); in masked hypertensive patients, -15.3±12.9/-9.3±9.5 mm Hg (n=1308, both P=0.000); and among white-coat hypertensive patients, 23.7±13.2/8.2±9.1 mm Hg (n=580, both P=0.000). Our results showed a difference of 5 mm Hg in SBP among sustained hypertensive patients, as recommended by the Japanese Society of Hypertension Guidelines for the Management of Hypertension; however, in other hypertensive patient types, the differences in SBP and DBP between office and home measurements differed by >5 mm Hg. Office and home BP measurements should be interpreted cautiously, keeping in mind the clinical setting.
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- 2016
10. Lack of TRPV2 impairs thermogenesis in mouse brown adipose tissue
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Shigeo Wakabayashi, Teruo Kawada, Yasunori Takayama, Kunitoshi Uchida, Wu-Ping Sun, Min-Ji Kim, Makoto Tominaga, Yiming Zhou, Yoshiro Suzuki, Nobuyuki Takahashi, Tsuyoshi Goto, and Yuko Iwata
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0301 basic medicine ,medicine.medical_specialty ,Autocommentary ,TRPV2 ,Adipose tissue ,TRPV Cation Channels ,White adipose tissue ,Biology ,Diet, High-Fat ,Biochemistry ,03 medical and health sciences ,Transient receptor potential channel ,Mice ,0302 clinical medicine ,Adipose Tissue, Brown ,Internal medicine ,Receptors, Adrenergic, beta ,Brown adipose tissue ,Genetics ,medicine ,Animals ,Obesity ,Molecular Biology ,Cells, Cultured ,PRDM16 ,Voltage-dependent calcium channel ,Cell Differentiation ,Thermogenesis ,Articles ,Mice, Inbred C57BL ,Adipocytes, Brown ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,Calcium ,Calcium Channels ,030217 neurology & neurosurgery - Abstract
Brown adipose tissue (BAT), a major site for mammalian non-shivering thermogenesis, could be a target for prevention and treatment of human obesity. Transient receptor potential vanilloid 2 (TRPV2), a Ca(2+)-permeable non-selective cation channel, plays vital roles in the regulation of various cellular functions. Here, we show that TRPV2 is expressed in brown adipocytes and that mRNA levels of thermogenic genes are reduced in both cultured brown adipocytes and BAT from TRPV2 knockout (TRPV2KO) mice. The induction of thermogenic genes in response to β-adrenergic receptor stimulation is also decreased in TRPV2KO brown adipocytes and suppressed by reduced intracellular Ca(2+) concentrations in wild-type brown adipocytes. In addition, TRPV2KO mice have more white adipose tissue and larger brown adipocytes and show cold intolerance, and lower BAT temperature increases in response to β-adrenergic receptor stimulation. Furthermore, TRPV2KO mice have increased body weight and fat upon high-fat-diet treatment. Based on these findings, we conclude that TRPV2 has a role in BAT thermogenesis and could be a target for human obesity therapy.
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- 2016
11. Gain-of-function haplotype in the epithelial calcium channel TRPV6 is a risk factor for renal calcium stone formation
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Andreas Pasch, Felix J. Frey, Olivier Bonny, Yoshiro Suzuki, Markus G. Mohaupt, and Matthias A. Hediger
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Adult ,Male ,medicine.medical_specialty ,TRPV6 ,Calcitriol ,Xenopus ,Hypercalciuria ,030232 urology & nephrology ,TRPV Cation Channels ,chemistry.chemical_element ,Parathyroid hormone ,Calcium ,Biology ,Polymorphism, Single Nucleotide ,Kidney Calculi ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,Animals ,Calcitriol/blood ,Calcium/analysis ,Calcium/blood ,Calcium Channels/genetics ,Calcium Channels/metabolism ,Female ,Haplotypes ,Humans ,Hypercalciuria/genetics ,Hypercalciuria/metabolism ,Kidney Calculi/chemistry ,Kidney Calculi/genetics ,Middle Aged ,Parathyroid Hormone/blood ,TRPV Cation Channels/genetics ,TRPV Cation Channels/metabolism ,Genetics ,medicine ,Molecular Biology ,Genetics (clinical) ,030304 developmental biology ,0303 health sciences ,Voltage-dependent calcium channel ,Calcium channel ,Haplotype ,General Medicine ,medicine.disease ,Endocrinology ,chemistry ,Parathyroid Hormone ,Calcium Channels ,medicine.drug - Abstract
The rate-limiting step of dietary calcium absorption in the intestine requires the brush border calcium entry channel TRPV6. The TRPV6 gene was completely sequenced in 170 renal calcium stone patients. The frequency of an ancestral TRPV6 haplotype consisting of three non-synonymous polymorphisms (C157R, M378V, M681T) was significantly higher (P = 0.039) in calcium stone formers (8.4%; derived = 502, ancestral = 46) compared to non-stone-forming individuals (5.4%; derived = 645, ancestral = 37). Mineral metabolism was investigated on four different calcium regimens: (i) free-choice diet, (ii) low calcium diet, (iii) fasting and (iv) after a 1 g oral calcium load. When patients homozygous for the derived haplotype were compared with heterozygous patients, no differences were found with respect to the plasma concentrations of 1,25-vitamin D, PTH and calcium, and the urinary excretion of calcium. In one stone-forming patient, the ancestral haplotype was found to be homozygous. This patient had absorptive hypercalciuria. We therefore expressed the ancestral protein (157R+378V+681T) in Xenopus oocytes and found a significantly enhanced calcium permeability when tested by a (45)Ca(2+) uptake assay (7.11 +/- 1.93 versus 3.61 +/- 1.01 pmol/min/oocyte for ancestral versus derived haplotype, P < 0.01). These results suggest that the ancestral gain-of-function haplotype in TRPV6 plays a role in calcium stone formation in certain forms of absorptive hypercalciuria.
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- 2008
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12. Noninvasive Body Contouring by Focused Ultrasound: Safety and Efficacy of the Contour I Device in a Multicenter, Controlled, Clinical Study
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Hidenori Matsuda, Spencer A. Brown, Morkel J Otto, Yoshiro Suzuki, John L Burns, Steven A Teitelbaum, Yukio Shirakabe, and Junichiro Kubota
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Adult ,Male ,medicine.medical_specialty ,Therapeutic ultrasound ,business.industry ,Ultrasonic Therapy ,medicine.medical_treatment ,Ultrasound ,MEDLINE ,Pilot Projects ,Cosmetic Techniques ,Focused ultrasound ,Surgery ,Clinical trial ,Body contouring ,Humans ,Medicine ,Female ,Obesity ,Prospective Studies ,Radiology ,business ,Prospective cohort study - Abstract
The removal of unwanted body fat using a noninvasive technique is desirable to patients and physicians. The authors describe a controlled, multicenter, clinical trial assessing the safety and efficacy of a focused therapeutic ultrasound device for noninvasive body contouring.Eligible healthy adult subjects were enrolled to the experimental group or the control group at five sites. The experimental group received one treatment with the Contour I device (UltraShape Ltd., Tel Aviv, Israel) in the abdomen, thighs, or flanks and were evaluated over a 12-week period. Efficacy outcomes were reduction of circumference and fat thickness. Circumference reduction was compared with the untreated group and with an untreated area (thigh) within the treated group. Safety monitoring included laboratory testing (including serum lipids), pulse oximetry, and liver ultrasound.One hundred sixty-four subjects participated in the study (137 subjects in the experimental group and 27 in the control, untreated group). A single Contour I treatment was safe and well tolerated and produced a mean reduction of approximately 2 cm in treatment area circumference and approximately 2.9 mm in skin fat thickness. The majority of the effect was achieved within 2 weeks and was sustained at 12 weeks. No clinically significant changes in the measured safety parameters were recorded. Seven adverse events were reported, all of which were anticipated, mild, and resolved within the study period.The Contour I device provides a safe and effective noninvasive technology for body contouring.
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- 2007
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13. Association of Leukoaraiosis With Convalescent Rehabilitation Outcome in Patients With Ischemic Stroke
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Gen Sobue, Joe Senda, Naoki Ishiguro, Masahiko Kanamori, Yoshiro Suzuki, Yoshihiro Nishida, Hideo Kishimoto, Tomomitsu Kotake, Izumi Kadono, Keiichi Ito, and Masahisa Katsuno
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Male ,medicine.medical_specialty ,medicine.medical_treatment ,030204 cardiovascular system & hematology ,Brain Ischemia ,Brain ischemia ,03 medical and health sciences ,0302 clinical medicine ,Acute care ,Internal medicine ,Medicine ,Humans ,Stroke ,Aged ,Retrospective Studies ,Advanced and Specialized Nursing ,Aged, 80 and over ,Rehabilitation ,medicine.diagnostic_test ,business.industry ,Leukoaraiosis ,Stroke Rehabilitation ,Magnetic resonance imaging ,Convalescence ,Middle Aged ,medicine.disease ,Functional Independence Measure ,Treatment Outcome ,Embolism ,Cardiology ,Physical therapy ,Female ,Neurology (clinical) ,Cardiology and Cardiovascular Medicine ,business ,030217 neurology & neurosurgery - Abstract
Background and Purpose— We investigated the factors influencing inpatient convalescent rehabilitation outcomes in patients with ischemic stroke, particularly severity of leukoaraiosis on magnetic resonance imaging. Methods— Participants included 520 patients with ischemic stroke (317 men and 203 women; mean age, 72.8±8.4 years) who were transferred from acute care hospitals for inpatient convalescent rehabilitation. Ischemic stroke subtypes included lacunar infarction (n=41), atherothrombosis (n=223), artery-to-artery embolism (n=67), cardiogenic embolism (n=97), undetermined embolism (n=76), and uncategorized ischemic stroke (n=16). Leukoaraiosis was graded according to periventricular hyperintensity (PVH) and deep white matter hyperintensity on magnetic resonance imaging. Functional Independence Measure scores were assessed on admission and at discharge. Results— Multiple regression analysis revealed that rehabilitation outcomes, measured as total Functional Independence Measure scores, were significantly associated with leukoaraiosis estimated by PVH grade. This association was observed after adjustment for factors such as severity, age, and poststroke history. In all patients, PVH grades were associated with Functional Independence Measure motor scores ( P P Conclusions— Our study revealed that the degree of leukoaraiosis was associated with inpatient convalescent rehabilitation outcome in patients with ischemic stroke. Furthermore, the PVH grade was associated with motor function outcome, whereas the deep white matter hyperintensity grade correlated with cognitive function outcome, likely because the progression patterns and anatomic backgrounds of PVH and deep white matter hyperintensity differ according to ischemic stroke subtype.
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- 2015
14. Marked Disturbance of Calcium Homeostasis in Mice With Targeted Disruption of the Trpv6 Calcium Channel Gene
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Claudine H. Kos, Alessandra Crescenzi, Michael R. Freeman, Matthias A. Hediger, Liyan Zhuang, Hongyu Luo, Jiangping Wu, Cecilia H. A. Gouveia, Suzy D.C. Bianco, Hitomi Takanaga, Yoshiro Suzuki, Theodora M. Mauro, Edward M. Brown, and Ji-Bin Peng
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medicine.medical_specialty ,TRPV6 ,Endocrinology, Diabetes and Metabolism ,TRPV Cation Channels ,Parathyroid hormone ,chemistry.chemical_element ,Biology ,Calcium ,Polymerase Chain Reaction ,Article ,Intestinal absorption ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Homeostasis ,Orthopedics and Sports Medicine ,RNA, Messenger ,DNA Primers ,030304 developmental biology ,Mice, Knockout ,2. Zero hunger ,Calcium metabolism ,0303 health sciences ,Base Sequence ,Voltage-dependent calcium channel ,Calcium channel ,Endocrinology ,Intestinal Absorption ,chemistry ,Parathyroid Hormone ,030220 oncology & carcinogenesis ,Calcium Channels - Abstract
We report the phenotype of mice with targeted disruption of the Trpv6 (Trpv6 KO) epithelial calcium channel. The mice exhibit disordered Ca(2+) homeostasis, including defective intestinal Ca(2+) absorption, increased urinary Ca(2+) excretion, decreased BMD, deficient weight gain, and reduced fertility. Although our Trpv6 KO affects the closely adjacent EphB6 gene, the phenotype reported here is not related to EphB6 dysfunction. INTRODUCTIOn: The mechanisms underlying intestinal Ca(2+) absorption are crucial for overall Ca(2+) homeostasis, because diet is the only source of all new Ca(2+) in the body. Trpv6 encodes a Ca(2+)-permeable cation channel responsible for vitamin D-dependent intestinal Ca(2+) absorption. Trpv6 is expressed in the intestine and also in the skin, placenta, kidney, and exocrine organs. MATERIALS AND METHODS: To determine the in vivo function of TRPV6, we generated mice with targeted disruption of the Trpv6 (Trpv6 KO) gene. RESULTS: Trpv6 KO mice are viable but exhibit disordered Ca(2+) homeostasis, including a 60% decrease in intestinal Ca(2+) absorption, deficient weight gain, decreased BMD, and reduced fertility. When kept on a regular (1% Ca(2+)) diet, Trpv6 KO mice have deficient intestinal Ca(2+) absorption, despite elevated levels of serum PTH (3.8-fold) and 1,25-dihydroxyvitamin D (2.4-fold). They also have decreased urinary osmolality and increased Ca(2+) excretion. Their serum Ca(2+) is normal, but when challenged with a low (0.25%) Ca(2+) diet, Trpv6 KO mice fail to further increase serum PTH and vitamin D, ultimately developing hypocalcemia. Trpv6 KO mice have normal urinary deoxypyridinoline excretion, although exhibiting a 9.3% reduction in femoral mineral density at 2 months of age, which is not restored by treatment for 1 month with a high (2%) Ca(2+) "rescue" diet. In addition to their deranged Ca(2+) homeostasis, the skin of Trpv6 KO mice has fewer and thinner layers of stratum corneum, decreased total Ca(2+) content, and loss of the normal Ca(2+) gradient. Twenty percent of all Trpv6 KO animals develop alopecia and dermatitis. CONCLUSIONS: Trpv6 KO mice exhibit an array of abnormalities in multiple tissues/organs. At least some of these are caused by tissue-specific mechanisms. In addition, the kidneys and bones of Trpv6 KO mice do not respond to their elevated levels of PTH and 1,25-dihydroxyvitamin D. These data indicate that the TRPV6 channel plays an important role in Ca(2+) homeostasis and in other tissues not directly involved in this process.
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- 2006
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15. A New Paradigm for the Aging Asian Face
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Yukio Shirakabe, Yoshiro Suzuki, and Samuel M. Lam
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Male ,Aging ,medicine.medical_specialty ,business.industry ,media_common.quotation_subject ,Eyebrow ,Illusion ,Face (sociological concept) ,Chin ,Surgery ,medicine.anatomical_structure ,Adipose Tissue ,Asian People ,Aesthetics ,Face ,Beauty ,Rhytidoplasty ,medicine ,Humans ,Rejuvenation ,Female ,business ,media_common ,Gravitational force - Abstract
Traditionally, Asians have been thought to age more gracefully than Caucasians. The resistance to aging in the Asian patient was credited to the thicker dermis of Asian skin that contains greater collagen and the darker pigment that protects against photoaging. Although these statements are true, the authors propose a new paradigm that explains how the illusion of Asian youthfulness may be understood. The "baby model" purports that the Asian face has many attributes similar to an infant, including a wider and rounder face, higher eyebrow, fuller upper lid, lower nasal bridge, flatter midface, apparently more protuberant lips, and more receded chin. These commonalities between the infant and the Asian compel the viewer to perceive the Asian face as more youthful. However, the Asian face is subjected to a greater amount of gravitational force due to weaker skeletal support, heavier soft tissue, larger amount of malar fat, thicker skin, and a weaker chin. Facial rejuvenative surgery should always be cognizant of the propensity of the Asian skin to unfavorable healing, need for greater tissue suspension, and more conspicuous temporal alopecia. Asian aesthetics that differ and converge with Western ideals are reviewed so that the Western surgeon in particular can comprehend the Asian conception of youthful beauty.
- Published
- 2003
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16. Expression of the K+ channel Kir7.1 in the developing rat kidney: Role in K+ excretion
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Yukiko Yasuoka, Yoshiro Suzuki, Katsumasa Kawahara, Nobuhiro Nakamura, Takao Shimohama, Shigeshisa Hirose, and Mariko Nishikitani
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medicine.medical_specialty ,Potassium Channels ,ATPase ,Biology ,Kidney ,cortical collecting duct ,Excretion ,neonatal kidney ,Internal medicine ,medicine ,Animals ,RNA, Messenger ,renal K+ excretion ,Distal convoluted tubule ,Potassium Channels, Inwardly Rectifying ,Na+/K+-ATPase ,urinary potassium ,inward rectifier K+ channel ,K+-ATPase ,Gene Expression Regulation, Developmental ,Na+ ,Immunohistochemistry ,Potassium channel ,Rats ,medicine.anatomical_structure ,Endocrinology ,Animals, Newborn ,Nephrology ,Renal physiology ,Potassium ,biology.protein ,ROMK ,Hyperkalemia ,Sodium-Potassium-Exchanging ATPase ,potassium channel - Abstract
Background Coordinated expression of ROMK (luminal K + channel in the thick ascending limb and the collecting duct) and Na + ,K + -ATPase has been demonstrated to be involved in the postnatal development of renal K + excretion; however, the developmental expression of the basolateral K + channel Kir7.1 is unknown. The purpose of this study was to elucidate the possible involvement of Kir7.1 in the maturation of renal K + excretion. Methods Developmental changes in the renal K + excretion under the condition of K + overload was investigated by collecting urine from neonatal rats infused with K + (KCl solution). RNase protection analysis was used to elucidate the expression of Kir7.1 and Na + ,K + -ATPase mRNA in pre- and postnatal rats, and the expression of Kir7.1 and ROMK mRNA at 7, 14, and 21 days. Western blotting of Kir7.1, and immunohistochemistry of Kir7.1 and ROMK were used to determine their protein expression. Results The ratio of urinary K + excretion to K + load increased between 7 and 14 days after birth. In addition, half excretion time of K + load gradually decreased through the experimental period of 7 and 21 days. Na + ,K + -ATPase mRNA levels showed a peak of up-regulation at birth that remained elevated. ROMK1 mRNA levels significantly increased between 7 and 14 days. On the other hand, Kir7.1 mRNA and protein levels significantly increased between 14 and 21 days. Kir7.1 protein in the thick ascending limb was first recognized at 7 days, whereas its expression in the distal convoluted tubule and the cortical collecting duct was found in 21-day-old neonates. Conclusion Our results suggest that Kir7.1 is involved in the development of renal K + excretion between 14 and 21 days after birth under the condition of K + overload.
- Published
- 2003
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17. Relationships between obesity and metabolic hormones in the 'cobalt' variant of rainbow trout
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Yoshiro Suzuki, Akiyoshi Takahashi, Takashi Yada, Shigehisa Hirose, Teruo Azuma, Shunsuke Moriyama, and Nobuko Naito
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Fish Proteins ,endocrine system ,medicine.medical_specialty ,animal structures ,Lipolysis ,animal diseases ,medicine.medical_treatment ,Triacylglycerol lipase ,digestive system ,Fish Diseases ,Endocrinology ,Internal medicine ,medicine ,Animals ,Insulin ,Obesity ,Triglycerides ,Glycoproteins ,biology ,urogenital system ,Pars intermedia ,Blood Proteins ,Lipase ,biology.organism_classification ,Prolactin ,Pituitary Hormones ,Trout ,Cholesterol ,Liver ,alpha-MSH ,Growth Hormone ,Infertility ,Oncorhynchus mykiss ,Pituitary Gland ,Animal Science and Zoology ,Rainbow trout ,Hormone - Abstract
The "cobalt" variant of rainbow trout (Oncorhynchus mykiss) lacks most of the pars intermedia of the pituitary, and shows significant obesity with an enlarged liver and a fat accumulation in the abdominal cavity. Plasma levels of growth hormone, prolactin, and somatolactin were significantly lower in the cobalt variant than those in the normal trout. In contrast, plasma insulin level was four times higher than that in the normal. Plasma levels of total protein, free cholesterol, and triacylglycerol were higher in the cobalt, while those of glucose and fatty acids were not different from the normal levels. In the white muscle, red muscle, liver, and mesenteric fat, the cobalt showed higher contents of triacylglycerol than the normal fish. There was no significant difference in tissue contents of phosphatidylcholine between the two groups of the trout, except for that in the mesenteric fat, exhibiting significantly lower content than in the normal fish. Activity of triacylglycerol lipase in the liver in vivo was lower in the cobalt than that in the normal trout, while there was no significant difference between the two in the cultured liver slices. Desacetyl-alpha-MSH stimulated lipolysis of triacylglycerol similarly in the cultured liver slices from the normal trout and from the cobalt variant. Results from this study suggest that the lack of pars intermedia and the increased plasma level of insulin are involved in a depression of lipid mobilization and obesity in this variant of rainbow trout.
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- 2002
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18. Inwardly rectifying K+ channel Kir7.1 is highly expressed in thyroid follicular cells, intestinal epithelial cells and choroid plexus epithelial cells: implication for a functional coupling with Na+,K+-ATPase
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Hidenari Sakuta, Kayoko Ookata, Katsumasa Kawahara, Nobuhiro Nakamura, Yoshiro Suzuki, and Shigehisa Hirose
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Male ,Epithelial sodium channel ,medicine.medical_specialty ,Potassium Channels ,Molecular Sequence Data ,Thyroid Gland ,Gene Expression ,Biology ,Biochemistry ,Follicular cell ,Cell Line ,Pregnancy ,Internal medicine ,medicine ,Animals ,Humans ,Tissue Distribution ,Amino Acid Sequence ,Intestinal Mucosa ,Potassium Channels, Inwardly Rectifying ,Na+/K+-ATPase ,Molecular Biology ,Epithelial polarity ,Sequence Homology, Amino Acid ,Thyroid ,Epithelial Cells ,Cell Biology ,Apical membrane ,Immunohistochemistry ,Potassium channel ,Rats ,Cell biology ,medicine.anatomical_structure ,Endocrinology ,Choroid Plexus ,Female ,Choroid plexus ,Sodium-Potassium-Exchanging ATPase ,Research Article - Abstract
A novel inwardly rectifying K+ channel, Kir7.1, with unique pore properties, was cloned recently. Working in the field of osmoregulation, we have also identified the same human and rat channel and found that the channel is unique not only in its pore sequence but also in its dense localization in the follicular cells of the thyroid gland. Northern blot analysis revealed that the channel message was abundantly expressed in the thyroid gland and small intestine, and moderately in the kidney, stomach, spinal cord and brain. Immunohistochemistry of the rat thyroid, intestine and choroid plexus demonstrated the expression of the channel protein in the follicular cells and epithelial cells, suggesting a role in the regulation of the ion-transporting functions of these specialized cells. The unique pore properties of Kir7.1 make it a strong candidate for the hypothetical low-conductance K+ channel that is functionally coupled with Na+,K+-ATPase by recycling K+. We therefore further examined the co-localization of Kir7.1 and Na+,K+-ATPase and found that both are localized in the basolateral membrane of the thyroid follicular cell; in the choroid plexus, which is known to be unique in having Na+,K+-ATPase in the apical side of the epithelial cells, Kir7.1 was found in the apical membrane, implying a close functional coupling between the channel and Na+,K+-ATPase.
- Published
- 1999
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19. Practical Rehabilitation and Treatments for Femoral Neck Fractures
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Morio Kawamura, Yoshiro Suzuki, Kunio Ida, and Akiyasu Chiba
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medicine.medical_specialty ,Rehabilitation ,business.industry ,medicine.medical_treatment ,Medicine ,business ,Femoral Neck Fractures ,Surgery - Published
- 1997
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20. Zinc transporters in prostate cancer
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Matthias A. Hediger, P. Anderle, Michael R. Freeman, Marc Bürzle, Yoshiro Suzuki, Marie-Christine Franz, and Gergely Kovacs
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Male ,medicine.medical_specialty ,Clinical Biochemistry ,Citric Acid Cycle ,Intracellular zinc ,chemistry.chemical_element ,Zinc ,Biology ,Biochemistry ,Models, Biological ,Article ,Prostate cancer ,Prostate ,Internal medicine ,medicine ,Advanced disease ,Humans ,Molecular Biology ,Cation Transport Proteins ,Prostatic Neoplasms ,Transporter ,General Medicine ,medicine.disease ,Citric acid cycle ,Gene Expression Regulation, Neoplastic ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Apoptosis ,Cancer research ,Molecular Medicine ,Energy Metabolism - Abstract
Prostate cancer is a major health concern as it has the second highest incidence rate among cancers in men. Despite progress in tumor diagnostics and therapeutic approaches, prognosis for men with advanced disease remains poor. In this review we provide insight into the changes of the intermediary metabolism in normal prostate and prostate cancer. In contrast to normal cells, prostate cancer cells are reprogrammed for optimal energy-efficiency with a functional Krebs cycle and minimal apoptosis rates. A key element in this relationship is the uniquely high zinc level of normal prostate epithelial cells. Zinc is transported by the SLC30 and SLC39 families of zinc transporters. However, in prostate cancer the intracellular zinc content is remarkably reduced and expression levels of certain zinc transporters are altered. Here, we summarize the role of different zinc transporters in the development of prostate cancer.
- Published
- 2013
21. Calcium channel TRPV6 is involved in murine maternal-fetal calcium transport
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Ji-Bin Peng, Yoshiro Suzuki, Christopher S. Kovacs, Matthias A. Hediger, Hitomi Takanaga, and Christopher P. Landowski
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Calbindins ,Heterozygote ,medicine.medical_specialty ,TRPV6 ,Placenta ,Pregnancy Trimester, Third ,Endocrinology, Diabetes and Metabolism ,TRPV Cation Channels ,chemistry.chemical_element ,Biology ,Calcium ,S100 Calcium Binding Protein G ,Calbindin ,Mice ,03 medical and health sciences ,Fetus ,0302 clinical medicine ,Pregnancy ,Fetal membrane ,Internal medicine ,medicine ,Animals ,Orthopedics and Sports Medicine ,RNA, Messenger ,Yolk sac ,Maternal-Fetal Exchange ,Yolk Sac ,030304 developmental biology ,Minerals ,0303 health sciences ,Voltage-dependent calcium channel ,Dissection ,Calcium channel ,Biological Transport ,Original Articles ,Amniotic Fluid ,Mice, Inbred C57BL ,Protein Transport ,medicine.anatomical_structure ,Endocrinology ,Gene Expression Regulation ,chemistry ,030220 oncology & carcinogenesis ,embryonic structures ,Female ,Calcium Channels - Abstract
Maternal-fetal calcium (Ca(2+)) transport is crucial for fetal Ca(2+) homeostasis and bone mineralization. In this study, the physiological significance of the transient receptor potential, vanilloid 6 (TRPV6) Ca(2+) channel in maternal-fetal Ca(2+) transport was investigated using Trpv6 knockout mice. The Ca(2+) concentration in fetal blood and amniotic fluid was significantly lower in Trpv6 knockout fetuses than in wildtypes. The transport activity of radioactive Ca(2+) ((45)Ca) from mother to fetuses was 40% lower in Trpv6 knockout fetuses than in wildtypes. The ash weight was also lower in Trpv6 knockout fetuses compared with wildtype fetuses. TRPV6 mRNA and protein were mainly localized in intraplacental yolk sac and the visceral layer of extraplacental yolk sac, which are thought to be the places for maternal-fetal Ca(2+) transport in mice. These expression sites were co-localized with calbindin D(9K) in the yolk sac. In wildtype mice, placental TRPV6 mRNA increased 14-fold during the last 4 days of gestation, which coincides with fetal bone mineralization. These results provide the first in vivo evidence that TRPV6 is involved in maternal-fetal Ca(2+) transport. We propose that TRPV6 functions as a Ca(2+) entry pathway, which is critical for fetal Ca(2+) homeostasis.
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- 2008
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22. Mechanisms and regulation of epithelial Ca2+ absorption in health and disease
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Christopher P. Landowski, Matthias A. Hediger, and Yoshiro Suzuki
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Intestinal Absorption/physiology ,medicine.medical_specialty ,TRPV6 ,Calcium/metabolism ,TRPV5 ,Physiology ,Hypercalciuria ,TRPV Cation Channels ,030209 endocrinology & metabolism ,Biology ,Epithelium ,Bone resorption ,Intestinal absorption ,Bone Diseases, Metabolic/metabolism ,03 medical and health sciences ,0302 clinical medicine ,SDG 3 - Good Health and Well-being ,Internal medicine ,medicine ,Homeostasis ,Animals ,Humans ,030304 developmental biology ,0303 health sciences ,Reabsorption ,Homeostasis/physiology ,Renal Reabsorption ,medicine.disease ,Epithelium/metabolism ,Bone Diseases, Metabolic ,Endocrinology ,Intestinal Absorption ,Hypercalciuria/metabolism ,Calcium ,TRPV Cation Channels/metabolism - Abstract
Ca2+ is essential for numerous physiological functions in our bodies. Therefore, its homeostasis is finely maintained through the coordination of intestinal absorption, renal reabsorption, and bone resorption. The Ca2+-selective epithelial channels TRPV5 and TRPV6 have been identified, and their physiological roles have been revealed: TRPV5 is important in final renal Ca2+ reabsorption, and TRPV6 has a key role in intestinal Ca2+ absorption. The TRPV5 knockout mice exhibit renal leak hypercalciuria and accordingly upregulate their intestinal TRPV6 expression to compensate for their negative Ca2+ balance. In contrast, despite their severe negative Ca2+ balance, TRPV6-null mice do not display any compensatory mechanism, thus resulting in secondary hyperparathyroidism. These results indicate that the genes for TRPV5 and TRPV6 are differentially regulated in human diseases associated with disturbed Ca2+ balance such as hypercalciuria, osteoporosis, and vitamin D–resistant rickets.
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- 2008
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23. P-423: Survey of prescribing data of older adults at dispensing pharmacies
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Masafumi Kuzuya, T. Hirose, M. Sakakibara, Yoshiro Suzuki, and Masahiro Akishita
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medicine.medical_specialty ,business.industry ,Family medicine ,medicine ,Pharmacy ,Geriatrics and Gerontology ,Hospital pharmacy ,business ,Gerontology - Published
- 2015
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24. Establishment of a mouse macula densa cell line with an nNOS promoter driving EGFP expression
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Hideaki Kawada, Yukiko Yasuoka, Yoshiro Suzuki, Hitoshi Endou, Katsumasa Kawahara, Masahiro Sato, and Masuo Obinata
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medicine.medical_specialty ,SV40 large T antigen ,Time Factors ,Physiology ,Green Fluorescent Proteins ,Immunoblotting ,Fluorescent Antibody Technique ,Mice, Transgenic ,Nitric Oxide Synthase Type I ,Biology ,Immunofluorescence ,Transfection ,Green fluorescent protein ,Cell Line ,Mice ,Furosemide ,Internal medicine ,medicine ,Animals ,RNA, Messenger ,Transgenes ,Kidney Tubules, Distal ,Promoter Regions, Genetic ,Tubuloglomerular feedback ,medicine.diagnostic_test ,Reverse Transcriptase Polymerase Chain Reaction ,General Medicine ,Exons ,Molecular biology ,medicine.anatomical_structure ,Endocrinology ,Gene Expression Regulation ,Cell culture ,Symporter ,Macula densa - Abstract
We describe a unique method for establishing a functionally intact macula densa cell line from immortalized renal cells in culture. The macula densa is involved in the tubuloglomerular feedback (TGF) system in the kidney and specifically expresses neuronal nitric oxide synthase (nNOS). A 347 bp portion of the nNOS promoter was used to drive the expression of enhanced green fluorescence protein (EGFP). An immortalized distal tubule (DT) cell line was derived from distal tubules microdissected from the kidneys of SV40 large T antigen transgenic mice. Immunofluorescence labeling using an antibody against nNOS revealed no specific EGFP expression in immunofluorescence-negative DT cells. The established cell line (NE-MD) showed a time-dependent increase in signals of the nNOS protein when they were incubated with 12 microM furosemide (an inhibitor of Na(+)-K(+)-2Cl(-) symporter) for 5 h. In conclusion, this newly developed macula densa cell line will be useful in studies of the TGF stem.
- Published
- 2005
25. Decreased expression of Na+/H+ exchanger isoform 1 (NHE1) in non-infarcted myocardium after acute myocardial infarction
- Author
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Chiharu Noda, Katsumasa Kawahara, Takao Shimohama, Takashi Masuda, Hiroe Niwano, Tohru Izumi, Yoshiro Suzuki, and Kiyotaka Sato
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Male ,medicine.medical_specialty ,Sodium-Hydrogen Exchangers ,Myocardial Infarction ,Infarction ,Hemodynamics ,Down-Regulation ,Ventricular Function, Left ,Left coronary artery ,Diastole ,Internal medicine ,medicine.artery ,medicine ,Animals ,cardiovascular diseases ,Myocardial infarction ,RNA, Messenger ,Rats, Wistar ,business.industry ,Myocardium ,medicine.disease ,Rats ,Preload ,medicine.anatomical_structure ,cardiovascular system ,Cardiology ,Cardiology and Cardiovascular Medicine ,Ligation ,business ,Reperfusion injury ,Artery - Abstract
Although cardiac NHE1 is activated during myocardial ischemia and reperfusion injury, little is known about changes in expression in non-infarcted myocardium after acute myocardial infarction (AMI). The purpose of this study was to examine left ventricular function and region dependent NHE1 expression after myocardial infarction. Therefore, we produced two AMI models in rats, a small infarction model which was continuously ligated at the branches of the left coronary artery, and an extensive infarction model continuously ligated at the root of the artery. We examined NHE1 mRNA expression using RNase protection assay and protein levels using Western blot analysis in non-infarcted myocardium during the 24 hour period after AMI. The level of NHE1 mRNA and protein expression in the whole heart including the infarcted myocardium did not change after a small infarction. On the other hand, in the case of an extensive infarction, the levels of NHE1 mRNA and protein expression decreased significantly by 21.5% (P
- Published
- 2002
26. Development of renal potassium excretion capacity in the neonatal rat
- Author
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Yoshiro Suzuki, Mariko Nishikitani, Katsumasa Kawahara, Asako Kokubo, Naohiko Anzai, and Ibuki Izumida-Moriguchi
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Male ,medicine.medical_specialty ,Physiology ,Potassium ,chemistry.chemical_element ,Urine ,Kidney ,Equivalent ,Internal medicine ,medicine ,Animals ,Rats, Wistar ,Neonatal rat ,Potassium Excretion Rate ,Sodium ,General Medicine ,Rats ,Endocrinology ,chemistry ,Serum potassium ,Animals, Newborn ,Renal sodium excretion ,Renal potassium excretion ,Female - Abstract
We investigated the capacity of newborn rats to excrete an acute potassium load to understand the development of a renal potassium excretion system. Three groups of the rats (7-14 d) were used to collect urine periodically over 6 h after oral infusion of potassium: control (no potassium loading) and low- and high-potassium-loaded rats. In the low-potassium-loaded group, infused with about 0.6 microEq of potassium chloride/g body wt., the rate of renal potassium excretion increased from 0.08 plus minus 0.02 (7 d) to 0.13 plus minus 0.02 (10 d) and 0.21 plus minus 0.03 (14 d) microEq/h/g body wt. The high-potassium-loaded rats (1.5-2.8 microEq/g body wt. potassium load) excreted potassium at a higher rate of 0.18 +/- 0.05 (7 d), 0.30 +/- 0.02 (10 d), and 0.45 +/- 0.10 (14 d) microEq/h/g body wt. They excreted 77% (7 d), 76% (10 d), and 95% (14 d) of the potassium load. These values were much larger than the rate of 0.026 microEq/h/g body wt. of the control rats and of 0.08 microEq/h/g body wt., a mean potassium excretion rate during development from 7 to 14 d calculated from the data in the previous study (Kanno T et al.: J. Pediatr. Gastr. Nutr. 24: 242-252, 1997). In the same period, serum potassium concentration in the newborn rats decreased significantly (p0.01) from 7.2 +/- 0.1 (7 d) to 6.7 +/- 0.1 mEq/l (14 d). All these results suggest that a renal potassium excretion system in the rat develops at least in the second week of life, and its capacity is high enough to excrete the daily potassium intake.
- Published
- 2002
27. Localization of inward rectifier potassium channel Kir7.1 in the basolateral membrane of distal nephron and collecting duct
- Author
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Kayoko Ookata, Shigehisa Hirose, Kenjiro Kimura, Christopher S. Wilcox, Yoshiro Suzuki, Akihiro Tojo, and Nobuhiro Nakamura
- Subjects
Male ,Pathology ,medicine.medical_specialty ,Potassium Channels ,Biology ,Rats, Sprague-Dawley ,medicine ,Animals ,Intercalated Cell ,Tissue Distribution ,Distal convoluted tubule ,Kidney Tubules, Collecting ,Potassium Channels, Inwardly Rectifying ,Microscopy, Immunoelectron ,Epithelial polarity ,Dose-Response Relationship, Drug ,Inward-rectifier potassium ion channel ,Potassium, Dietary ,General Medicine ,Nephrons ,Connecting tubule ,Cell biology ,Rats ,medicine.anatomical_structure ,Ion homeostasis ,Aquaporin 3 ,Nephrology ,ROMK - Abstract
Inward rectifier potassium channels (Kir) play an important role in the K(+) secretion from the kidney. Recently, a new subfamily of Kir, Kir7.1, has been cloned and shown to be present in the kidney as well as in the brain, choroid plexus, thyroid, and intestine. Its cellular and subcellular localization was examined along the renal tubule. Western blot from the kidney cortex showed a single band for Kir7.1 at 52 kD, which was also observed in microdissected segments from the thick ascending limb of Henle, distal convoluted tubule (DCT), connecting tubule, and cortical and medullary collecting ducts. Kir7.1 immunoreactivity was detected predominantly in the DCT, connecting tubule, and cortical collecting duct, with lesser expression in the thick ascending limb of Henle and in the medullary collecting duct. Kir7.1 was detected by electron microscopic immunocytochemistry on the basolateral membrane of the DCT and the principal cells of cortical collecting duct, but neither type A nor type B intercalated cells were stained. The message levels and immunoreactivity were decreased under low-K diet and reversed by low-K diet supplemented with 4% KCl. By the double-labeling immunogold method, both Kir7.1 and Na(+), K(+)-ATPase were independently located on the basolateral membrane. In conclusion, the novel Kir7.1 potassium channel is located predominantly in the basolateral membrane of the distal nephron and collecting duct where it could function together with Na(+), K(+)-ATPase and contribute to cell ion homeostasis and tubular K(+) secretion.
- Published
- 2000
28. Effects of Desacetyl-alpha-MSH on Lipid Mobilization in the Rainbow Trout, Oncorhynchus mykiss
- Author
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Teruo Azuma, Takashi Yada, Shigehisa Hirose, Yoshiro Suzuki, and Akiyoshi Takahashi
- Subjects
chemistry.chemical_classification ,endocrine system ,medicine.medical_specialty ,animal structures ,integumentary system ,biology ,Fatty acid ,In vitro ,Endocrinology ,chemistry ,Internal medicine ,biology.protein ,medicine ,Lipolysis ,Animal Science and Zoology ,Rainbow trout ,Endorphins ,Lipase ,hormones, hormone substitutes, and hormone antagonists ,Hydrocortisone ,medicine.drug ,Hormone - Abstract
Effects of melanocyte-stimulating hormone (MSH) and beta-endorphin on lipid mobilization were examined in the rainbow trout (Oncorhynchus mykiss). Plasma levels of fatty acid (FA) were measured after intra-arterial administration of alpha-MSH, desacetyl-alpha-MSH, beta-MSH, or beta-endorphin through a cannula in the dorsal aorta. Desacetyl-alpha-MSH at 1 ng/g body weight resulted in an increase in plasma FA levels 1-3 hr after the injection, whereas the other three peptides showed no significant effect at the same dose. There was no significant change in plasma levels of cortisol after administration of any of the peptides. Lipolytic enzyme activity in the liver was significantly increased in a dose-related manner 1 hr after single intra-peritoneal injection of desacetyl-alpha-MSH. The direct effect of desacetyl-alpha-MSH on lipolysis was examined in liver slices incubated in vitro. Lipase activity in the liver slice was stimulated in the medium containing desacetyl-alpha-MSH in a dose-related manner. The results indicate that desacetyl-alpha-MSH is a potent stimulator of lipid mobilization in the rainbow trout.
- Published
- 2000
29. The Meckel syndrome: report of two Japanese sibs and a review of literature
- Author
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Masanori Kobayashi, Yoshiro Suzuki, and Yasuo Sugiura
- Subjects
Postaxial polydactyly ,Cystic kidney ,Male ,congenital, hereditary, and neonatal diseases and abnormalities ,medicine.medical_specialty ,Occipital encephalocele ,Polydactyly ,Foot Deformities, Congenital ,business.industry ,Infant, Newborn ,Anatomy ,medicine.disease ,Encephalocele ,Meckel Diverticulum ,Endocrinology ,Internal medicine ,medicine ,Humans ,Female ,Differential diagnosis ,Sibling ,Meckel syndrome ,business ,Hand Deformities, Congenital ,Genetics (clinical) - Abstract
Two Japanese sibs with the Meckel syndrome are reported. Both babies showed the classical triad of this condition : occipital encephalocele, cystic kidneys, and postaxial polydactyly of all four limbs. The diagnostic criteria and differential diagnosis were reviewed.
- Published
- 1996
30. Surgical treatment for nonspecific ulcer of the intestine with special respect to the surgical results and the problem of the recurrence
- Author
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Yuji Funayama, Morihiko Toda, Yasuhiko Kamiyama, Hiroo Naitou, Iwao Sasaki, Yoshiro Suzuki, and Mikio Imamura
- Subjects
Surgical results ,medicine.medical_specialty ,business.industry ,Gastroenterology ,Medicine ,Surgery ,business ,Surgical treatment - Abstract
昭和36年以来教室で経験した非特異性腸潰瘍25例の外科治療成績について再発例を中心とした検討を行った.その結果, 外科治療成績は一般に良好であるが, 25例中9例 (36%) に再発がみられ, なかには予後不良例も認められた.再発はU1-IIの小腸潰瘍は残存小腸に, U1-III~IVの小腸潰瘍および回盲部潰瘍では吻合部に発生する傾向があった.遠隔調査時における経過は良好で, 長期間の保存的治療にも改善のみられない症例には外科的治療を考慮してよいと思われた.一方, 再発を繰り返す難治例やいわゆる“非特異性多発性小腸潰瘍症”についてはCrohn病に準じ慎重に対処する必要があると思われた.
- Published
- 1986
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31. Intestinal microflora after continent ileostomy for patients with ulcerative colitis
- Author
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Hiroo Naito, Morihiko Toda, Yuji Fanayama, Hideo Narui, Iwao Sasaki, and Yoshiro Suzuki
- Subjects
medicine.medical_specialty ,Continent ileostomy ,business.industry ,Internal medicine ,Gastroenterology ,Medicine ,Surgery ,business ,medicine.disease ,Ulcerative colitis - Abstract
潰瘍性大腸炎に対する全大腸摘出兼回腸瘻造設でのcontinent ileostomy (以下, Kock法) 3例とend ileostomy4例を対象として糞便中の細菌叢について比較検討した. Kock法では総菌数および嫌気性菌数・好気性菌数の比ともend ileostomyに比べて健康人の糞便細菌叢に近い値を示した. 菌群についてみると, 好気性菌は両術式間で差を認めないが. 嫌気性菌はKock法が健康人に近かった. E. coliはKock法で全例検出されたが, end ileostomyでは4例中1例のみに検出された. 回腸瘻の腸内細菌叢についてみると, Kock法はend ileostomyに比べ大腸化の傾向が認められた.
- Published
- 1987
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32. Clinical study on the surgical treatment for ulcerative colitis in childhood
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Iwao Sasaki, Takashi Tsuchiya, Yoshiro Suzuki, Mitsuhiro Kato, Hideo Narui, Mikio Imamura, Yasuhiko Kamiyama, Hiroo Naito, Morihiko Toda, and Yuji Funayama
- Subjects
Clinical study ,medicine.medical_specialty ,business.industry ,Internal medicine ,Gastroenterology ,medicine ,Surgery ,Surgical treatment ,medicine.disease ,business ,Ulcerative colitis - Abstract
当教室で1961年より1985年までに経験した潰瘍性大腸炎手術例53例のうち手術時年齢15歳以下の小児期手術例5例について検討を加えた. 男女比は4: 1で平均年齢は13.4歳である. 全例が全大腸炎型, 重症例で (準) 緊急手術が3例に, 待期手術が2例に施行された. 手術死亡は14歳男性で他の4例は手術により早期に社会復帰し, 発育にも改善を認めた. これらの遠隔成績は比較的良好であり, 発育期の重要な時期にあっていたずらに保存的治療に拘泥すべきではなく積極的な外科治療を行ってよいと考えられた, また小児期の特殊性としてとくに精神面を含めた術後の遠隔時での管理の重要性が示唆された.
- Published
- 1987
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33. Blood Pressure Measurement of the Cerebral Artery with Strain Gauge Forceps
- Author
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Yoshiro Suzuki, Kenichiro Sugita, Naomi Mutsuga, Toshiyuki Hirota, Yuichiro Hayashi, Naoki Kageyama, and Tetsuro Mizutani
- Subjects
medicine.medical_specialty ,business.industry ,Carotid-Cavernous Sinus Fistula ,Hydrostatic pressure ,Forceps ,Cerebral arteries ,Anastomosis ,Surgery ,Blood pressure ,medicine.anatomical_structure ,medicine.artery ,cardiovascular system ,medicine ,Neurology (clinical) ,Internal carotid artery ,business ,Artery - Abstract
In order to measure blood pressure (BP) of the vessels without damage, forceps with a pair of strain gauges has been designed and the applicability to quantitative BP measurement with the forceps was examined. Using 42 mongrel dogs BP of various arteries was measured by the strain gauge forceps and intraarterial cannulation method simultaneously. When the force of forceps made the artery to collapse, it was called“Maximum Force of Forceps” (MFF). MFF was well proportional to the intraarterial cannulation BP. Small pieces of human cerebral arteries, obtained from 7 autopsies, were also examined by strain gauge forceps loading by hydrostatic pressure. From the results of measurement of human cerebral arteries in hydrostatic pressure, a formula was introduced. With the formula intraarterial pressure could be calculated from the diameter of artery and MFF. The formula was applied to measure the blood pressure during surgery of STA-MCA anastomosis, AVM, aneurysm and carotid cavernous sinus fistula. Success of vascular anastomosis could be demonstrated quantitatively with measuring blood pressure of cortical MCA in STA-MCA anastomosis before and after the procedure. The change of blood pressure of draining vessel could show the extent of obstruction of feeding arteries in each step of extirpation of AVM. In carotid cavernous sinus fistula, remarkable change of blood pressure of intracranial internal carotid artery could be discerned after trapping of the fistula. In aneurysm operation, blood pressure measurement of parent artery helps detecting the stenosis after clipping or ligation of wide neck aneurysm.
- Published
- 1977
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34. A case of gastro-duodenal Crohn's disease with pyloric stenosis
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Mikio Imamura, Mitinaga Takahashi, Yuji Funayama, Kouhei Fukushima, Iwao Sasaki, Yoshiro Suzuki, and Hiroo Naito
- Subjects
medicine.medical_specialty ,Gastro ,business.industry ,Internal medicine ,Gastroenterology ,medicine ,Surgery ,medicine.disease ,business ,Duodenal Crohn's disease ,Pyloric stenosis - Published
- 1987
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35. INFLUENCE OF A NEW ANTIULCER AGENT, AMMONIUM 7-OXOBICYCLO (2, 2, 1) HEPT-5-ENE-3-CARBAMOYL-2-CARBOXYLATE (KF-392) ON GASTRIC LESIONS AND GASTRIC MUCOSAL BARRIER IN RATS
- Author
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Yoshiro Suzuki, Tetsuo Kojima, Hirofuto Marumo, and Hiroshi Tanaka
- Subjects
Bridged-Ring Compounds ,Male ,medicine.medical_specialty ,Reserpine ,Buffers ,Gastroenterology ,Bridged Bicyclo Compounds ,Mice ,chemistry.chemical_compound ,Back diffusion ,Stress, Physiological ,Internal medicine ,Gastric mucosa ,Animals ,Medicine ,Ammonium ,Stomach Ulcer ,Carboxylate ,Ene reaction ,Pharmacology ,Gastric mucosal barrier ,Aspirin ,Gastric Juice ,business.industry ,Gastric lesions ,Anti-Ulcer Agents ,Bridged Bicyclo Compounds, Heterocyclic ,digestive system diseases ,Rats ,medicine.anatomical_structure ,chemistry ,Gastric Mucosa ,Charcoal ,Potentiometry ,business ,medicine.drug - Abstract
Antiulcer effects of KF-392 were studied in several experimental gastric ulcer models in rats. It was found that KF-392 given orally at 1.0 to 5.0 mg/kg had a marked suppression on the developments of Shay ulcer as well as the aspirin-, stress-, and reserpine-induced gastric lesions. The influence of KF-392 on gastric mucosal barrier was also studied. A back diffusion of H+ into the gastric mucosa and a fall of transmucosal potential difference were induced with KF-392 given orally at the above mentioned doses. KF-392 given s.c. at 5.0 mg/kg showed no inhibition on Shay ulcer and no induction of back diffusion of H+ into the gastric mucosa.
- Published
- 1976
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36. STUDIES ON THE FIBRINOGEN METABOLISM IN PATIENTS WITH VARIOUS DISEASES POSSIBLY COMPLICATED WITH HYPERCOAGULABLE STATE
- Author
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Yoshiro Suzuki
- Subjects
medicine.medical_specialty ,Endocrinology ,business.industry ,Internal medicine ,medicine ,In patient ,Metabolism ,Fibrinogen ,business ,medicine.drug - Published
- 1973
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37. STUDIES ON THE FIBRINOGEN METABOLISM IN THE RABBIT WITH EXPERIMENTALLY INDUCED HYPERCOAGULABLE STATE
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Yoshiro Suzuki
- Subjects
medicine.medical_specialty ,Endocrinology ,Chemistry ,Internal medicine ,medicine ,Rabbit (nuclear engineering) ,Metabolism ,Fibrinogen ,medicine.drug - Published
- 1973
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38. Mutations in the Tight-Junction Gene Claudin 19 (CLDN19) Are Associated with Renal Magnesium Wasting, Renal Failure, and Severe Ocular Involvement
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Marcel Schmid, Peter Nürnberg, Karl P. Schlingmann, Helga Vitzthum, Harald Jüppner, Martin Konrad, Sevcan A. Bakkaloglu, André Schaller, Stephan C.F. Neuhauss, Francisco Cano, Stefanie Weber, Juan Rodriguez-Soriano, Christian Becker, Dominik Seelow, Siegfried Waldegger, Amit V. Pandey, Annegret Lesslauer, Sabina Gallati, Ricardo Enriquez, John M. Luk, Gema Ariceta, Yoshiro Suzuki, and Matthias A. Hediger
- Subjects
Male ,Models, Molecular ,030232 urology & nephrology ,Kidney Failure, Chronic - genetics ,Kidney ,urologic and male genital diseases ,Mice ,0302 clinical medicine ,Genetics(clinical) ,Eye Abnormalities ,Child ,Genetics (clinical) ,0303 health sciences ,Chromosomes, Human, Pair 1 - genetics ,Tight junction ,Chromosome Mapping ,Middle Aged ,Recombinant Proteins ,Pedigree ,medicine.anatomical_structure ,Chromosomes, Human, Pair 1 ,Child, Preschool ,Female ,Adult ,medicine.medical_specialty ,Tight Junctions - genetics ,Membrane Proteins/chemistry - genetics - metabolism ,Adolescent ,Molecular Sequence Data ,Biology ,Magnesium Deficiency - genetics ,Hypomagnesemia ,Cell Line ,Tight Junctions ,03 medical and health sciences ,Dogs ,TRPM6 ,Internal medicine ,Report ,Genetics ,medicine ,Animals ,Humans ,Claudin ,030304 developmental biology ,urogenital system ,Kidney metabolism ,Membrane Proteins ,Renal magnesium wasting ,medicine.disease ,Endocrinology ,Claudins ,Mutation ,Cancer research ,Kidney Failure, Chronic ,Magnesium Deficiency ,Kidney disease - Abstract
Claudins are major components of tight junctions and contribute to the epithelial-harrier function by restricting free diffusion of solutes through the paracellular pathway. We have mapped a new locus for recessive renal magnesium loss on chromosome 1p34.2 and have identified mutations in CLDN19, a member of the claudin multigene family, in patients affected by hypomagnesemia, renal failure, and severe ocular abnormalities. CLDN19 encodes the tight-junction protein claudin-19, and we demonstrate high expression of CLDN19 in renal tubules and the retina. The identified mutations interfere severely with either cell-membrane trafficking or the assembly of the claudin-19 protein. The identification of CLDN19 mutations in patients with chronic renal failure and severe visual impairment supports the fundamental role of claudln-19 for normal renal tubular function and undisturbed organization and development of the retina. © 2006 by The American Society of Human Genetics. All rights reserved., published_or_final_version
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39. Dysphagia due to hypertrophic cervical osteophytes
- Author
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Yoshiro Suzuki, Takehiko Sakakibara, Hideo Hirano, Kikuo Inoue, Yoshinori Higuchi, and Hirotoshi Suzuki
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Male ,medicine.medical_specialty ,Thesaurus (information retrieval) ,business.industry ,General surgery ,General Medicine ,Middle Aged ,Dysphagia ,Radiography ,Spinal Osteophytosis ,Recurrence ,medicine ,Cervical Vertebrae ,Humans ,Orthopedics and Sports Medicine ,Surgery ,Female ,medicine.symptom ,business ,Deglutition Disorders - Published
- 1982
40. Intertrochanteric osteotomy and total hip replacement for bilateral osteoarthritis of the hip: consideration of the nonoperated-on hip
- Author
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Masashi Nakagawa, Sho Sugiura, Yoshiro Suzuki, Kunio Ida, and Hisashi Iwata
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Adult ,medicine.medical_specialty ,business.industry ,Radiography ,medicine.medical_treatment ,Total hip replacement ,General Medicine ,Osteoarthritis ,Middle Aged ,Osteotomy ,medicine.disease ,Intertrochanteric osteotomy ,Surgery ,medicine ,Humans ,Orthopedics and Sports Medicine ,Female ,Hip Joint ,Hip Prosthesis ,business - Abstract
In early stages of osteoarthritis, osteotomy produced a satisfactory result with no adverse effects and significant improvement in the nonoperated-on contralateral hip. In many cases of unilateral osteotomy in a group with preoperative radiologic scores above 70, the function was either improved or unchanged in the nonoperated-on hip. In many cases with scores below 70, there was deterioration of joint structure. Bilateral osteotomy is recommended only in the cases with preoperative radiologic scores below 70. Osteotomy was prescribed on the contralateral hip within a short period of time after aarthroplasty. Deterioration occurred in some contralateral nonoperated-on hips when the preoperative clinical scores were high, while improvement generally occurred when preoperative scores were low.
- Published
- 1980
41. Effect of ileo-jejunal transposition on intestinal adaptation after total colectomy in dogs
- Author
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Morihiko Toda, Yoshiro Suzuki, Toshio Sato, Hiroo Naito, Yuji Funayama, Takashi Tuchiya, Iwao Sasaki, and Akira Ohneda
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medicine.medical_specialty ,medicine.medical_treatment ,Glucagon-Like Peptides ,Ileum ,Enteroglucagon ,Gastroenterology ,General Biochemistry, Genetics and Molecular Biology ,Muscle hypertrophy ,Jejunum ,Dogs ,Intestinal mucosa ,Internal medicine ,Gastrins ,medicine ,Animals ,Intestinal Mucosa ,Colectomy ,Gastrin ,business.industry ,Body Weight ,General Medicine ,Hypertrophy ,Glucagon ,Adaptation, Physiological ,Intestines ,Postprandial ,medicine.anatomical_structure ,business ,Peptides - Abstract
The effect of ileo-jejunal transposition (IJT) on the intestinal adaptation after total colectomy was investigated in 4 mongrel dogs. Hyperenteroglucagonemia was observed in the IJT with colectomy group, especially in postprandial state. Obvious hyperplastic changes were observed in all part of the small intestinal mucosa in the colectomy with IJT group. However, there were no significant differences in body weight changes between the colectomy with IJT group and the colectomy group. Postprandial plasma gastrin levels were lower in the colectomy with IJT group compared to the control. These results suggest that IJT causes hyperenteroglucagonemia and intestinal mucosal hypertrophy in colectomized dogs. Enteroglucagon may have an inhibitory effect on postprandial gastrin release.
- Published
- 1987
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