1. Mouse Trmt2B protein is a dual specific mitochondrial metyltransferase responsible for m5U formation in both tRNA and rRNA
- Author
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Petr V. Sergiev, Marina V. Serebryakova, Ekaterina Shvetsova, Piotr Kamenski, Olga A. Dontsova, S. A. Levitskii, M. P. Rubtsova, I. G. Laptev, and Alexey A. Bogdanov
- Subjects
RNA, Mitochondrial ,Mitochondrion ,Biology ,Methylation ,Ribosome ,Cell Line ,Substrate Specificity ,Electron Transport Complex III ,Mice ,03 medical and health sciences ,0302 clinical medicine ,RNA, Transfer ,Animals ,Nucleotide ,Molecular Biology ,030304 developmental biology ,chemistry.chemical_classification ,tRNA Methyltransferases ,0303 health sciences ,Electron Transport Complex I ,Base Sequence ,Cell Biology ,Ribosomal RNA ,Mitochondria ,Biochemistry ,chemistry ,RNA, Ribosomal ,030220 oncology & carcinogenesis ,Small subunit ,Transfer RNA ,Nucleic Acid Conformation ,Thymine ,Research Paper - Abstract
RNA molecules of all species contain modified nucleotides and particularly m(5)U residues. The vertebrate mitochondrial small subunit rRNA contains m(5)U nucleotide in a unique site. In this work we found an enzyme, TRMT2B, responsible for the formation of this nucleotide and m(5)U residues in a number of mitochondrial tRNA species. Inactivation of the Trmt2B gene leads to a reduction of the activity of respiratory chain complexes I, III and IV, containing the subunits synthesized by the mitochondrial protein synthesis apparatus. Comparative sequence analysis of m(5)U-specific RNA methyltransferases revealed an unusual evolutionary pathway of TRMT2B formation which includes consecutive substrate specificity switches from the large subunit rRNA to tRNA and then to the small subunit rRNA.
- Published
- 2019