151. SmeOP-TolCSm Efflux Pump Contributes to the Multidrug Resistance of Stenotrophomonas maltophilia
- Author
-
Cheng Wen Lin, Rouh Mei Hu, Yi Wei Huang, and Tsuey Ching Yang
- Subjects
Nalidixic acid ,Stenotrophomonas maltophilia ,Microbial Sensitivity Tests ,Microbiology ,chemistry.chemical_compound ,Nalidixic Acid ,Bacterial Proteins ,Mechanisms of Resistance ,Drug Resistance, Multiple, Bacterial ,Operon ,medicine ,Pharmacology (medical) ,Sodium dodecyl sulfate ,Amikacin ,Pharmacology ,biology ,Membrane transport protein ,Reverse Transcriptase Polymerase Chain Reaction ,Membrane Transport Proteins ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Multiple drug resistance ,Infectious Diseases ,chemistry ,Doxycycline ,biology.protein ,bacteria ,Gentamicin ,Efflux ,Gentamicins ,medicine.drug - Abstract
A five-gene cluster, tolC Sm -pcm-smeRo-smeO-smeP , of Stenotrophomonas maltophilia was characterized. The presence of smeOP and smeRo-pcm-tolC Sm operons was verified by reverse transcription (RT)-PCR. Both operons were negatively regulated by the TetR-type transcriptional regulator SmeRo, as demonstrated by quantitative RT-PCR and a promoter-fusion assay. SmeO and SmeP were associated with TolC Sm (the TolC protein of S. maltophilia ) for the assembly of a resistance-nodulation-cell-division (RND)-type pump. The compounds extruded by SmeOP-TolC Sm mainly included nalidixic acid, doxycycline, amikacin, gentamicin, erythromycin, leucomycin, carbonyl cyanide 3-chlorophenylhydrazone, crystal violet, sodium dodecyl sulfate, and tetrachlorosalicylanilide.
- Published
- 2014