151. Control of ciliogenesis by FOR20, a novel centrosome and pericentriolar satellite protein
- Author
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Daniel Birnbaum, Aslıhan Tolun, Véronique Chevrier, Jean-Paul Chauvin, Fatima Sedjaï, Emilie Coppin, Michel Pierres, Claire Acquaviva, Olivier Rosnet, François Coulier, Aicha Aouane, Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC), Centre de Recherche en Cancérologie de Marseille (CRCM / U891 Inserm), Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Université de la Méditerranée - Aix-Marseille 2, Department of Molecular Biology and Genetics, Boaziçi University, Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)
- Subjects
Chromosomal Proteins, Non-Histone ,Cell Cycle Proteins ,Retinal Pigment Epithelium ,Protein Engineering ,Autoantigens ,Microtubules ,MESH: Centrosome ,MESH: Antibodies, Monoclonal ,0302 clinical medicine ,MESH: Gene Expression Regulation, Developmental ,MESH: RNA, Small Interfering ,Basal body ,MESH: Animals ,MESH: Proteins ,RNA, Small Interfering ,MESH: Phylogeny ,Phylogeny ,Cell Line, Transformed ,0303 health sciences ,MESH: Microtubules ,Cilium ,Antibodies, Monoclonal ,Gene Expression Regulation, Developmental ,Cell Differentiation ,MESH: Retinal Pigment Epithelium ,Cell biology ,MESH: Protein Engineering ,MESH: Autoantigens ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,MESH: Cell Differentiation ,MESH: Rats ,MESH: Hybridomas ,Biology ,Flagellum ,03 medical and health sciences ,PCM1 ,MESH: Cell Cycle Proteins ,Microtubule ,MESH: Chromosomal Proteins, Non-Histone ,MESH: Cilia ,Ciliogenesis ,Animals ,Humans ,Cilia ,MESH: Cell Line, Transformed ,030304 developmental biology ,Centrosome ,Hybridomas ,MESH: Humans ,Proteins ,Cell Biology ,Rats ,Centriolar satellite ,030217 neurology & neurosurgery - Abstract
International audience; Cilia and flagella are evolutionary conserved organelles that generate fluid movement and locomotion, and play roles in chemosensation, mechanosensation and intracellular signalling. In complex organisms, cilia are highly diversified, which allows them to perform various functions; however, they retain a 9+0 or 9+2 microtubules structure connected to a basal body. Here, we describe FOR20 (FOP-related protein of 20 kDa), a previously uncharacterized and highly conserved protein that is required for normal formation of a primary cilium. FOR20 is found in PCM1-enriched pericentriolar satellites and centrosomes. FOR20 contains a Lis1-homology domain that promotes self-interaction and is required for its satellite localization. Inhibition of FOR20 expression in RPE1 cells decreases the percentage of ciliated cells and the length of the cilium on ciliated cells. It also modifies satellite distribution, as judged by PCM1 staining, and displaces PCM1 from a detergent-insoluble to a detergent-soluble fraction. The subcellular distribution of satellites is dependent on both microtubule integrity and molecular motor activities. Our results suggest that FOR20 could be involved in regulating the interaction of PCM1 satellites with microtubules and motors. The role of FOR20 in primary cilium formation could therefore be linked to its function in regulating pericentriolar satellites. A role for FOR20 at the basal body itself is also discussed.
- Published
- 2010