Search

In order to explore the grouting materials suitable for the fracture reinforcement of Jinsha soil site, three kinds of grouting materials were prepared by mixing calcined ginger nut, SH and inorganic mineral polymer with the test soil of Jinsha site. Through laboratory tests, the basic physical properties, mechanical properties and durability of various grouting materials were analyzed in detail. The results show that the inorganic mineral polymer grouting material has low shrinkage and can effectively fill the cracks. At the same time, its high flexural and compressive strength provides a reliable guarantee for improving the mechanical stability of earthen sites. Among the three grouting materials studied, the inorganic mineral polymer grouting material exhibits the best durability. Therefore, it is recommended to use inorganic mineral polymer grouting materials in the crack grouting reinforcement project of Jinsha soil site. The research results will provide useful guidance for the selection and application of crack grouting materials in soil sites under humid environment. [ABSTRACT FROM AUTHOR]

The preparation mechanism and liquefaction mechanism is reviewed, then the preparation method and modification mechanism of bio-asphalt is given, and the research situation and development prospects of bio-asphalt are concluded at home and abroad.Compared with traditional asphalt, bio-asphalt has better road performance, economic and environmental benefits, and could be used as a good substitute for traditional road asphalt. [ABSTRACT FROM AUTHOR]

Objective：To investigate the role of microbes in colorectal cancer liver metastases(CRLM)and the underlying mechanisms. Methods：16S rDNA sequencing was performed on colon tumors and liver tumors in CRLM patients. The mouse model of CRLM was established and treated with antibiotics, Escherichia coli(E.coli), lactate dehydrogenase inhibitor and clodronate liposome. After 24 days, the liver tumor tissues were obtained for HE staining, immunofluorescence staining and detection of lactate concentration and microbial abundance. E. coli and MC38 cells were co - cultured at 100∶1. The cell proliferation, the extracellular acidification rate(ECAR)and concentration of lactate was determined by CCK8, Seahorse and colorimetry respectively. Macrophages (MØ)derived from mouse bone marrow were treated with lactate, lactylation antibody and TNF-α respectively. The expression levels of CD206 and iNOS were detected by immunofluorescence；the mRNA levels of iNOS, CD86, CD163 and ARG were detected by qPCR; The lactylation level and NF-κB expression were detected by Western Blot. Results：There were microbes in colon and liver tumors in CRLM patients and the dominant species were similar. Microbiota promoted liver metastasis of colorectal cancer in mice and MØ was found to be M2 - like polarized, while the liver metastases decreased after depletion of MØ. Microbiota could not enhance the proliferation of tumor cells, but could increase the level of tumor glycolysis and lactate and inhibition of lactate dehydrogenase could reduce liver metastases in mice.Lactate could induce MØ polarization to M2 and lactylation. RIG - Ilys852 was found to inhibit the polarization of MØ induced by lactate;the expression of NF -κB was decreased after macrophage treated with lactate;TNF -α could inhibit MØ polarization to M2. Conclusion:Microbes enhance the level of glycolysis and lactate in tumor and promote liver metastasis of colorectal cancer via macrophage RIG-I lactylation. [ABSTRACT FROM AUTHOR]

Combined with mass spectrometry, ion spectroscopy can obtain a spectrum of the target ion by measuring its intensity change, which is induced by laser strength and wavelength. It integrates the high sensitivity of mass spectrometry and the structural specificity of spectroscopy, and thus proves to be very useful in structure characterization of complex biomolecules, such as proteins, peptides, glycans, oligonucleotides, etc. However, the electronic or vibrational spectra becomes too complex to be resolved when analyzing isomeric ions. Ion mobility spectrometry (IMS) allows ionized molecules to be separated on the basis of mobilities in the gas phase, and its coupling with electrospray and MALDI ion sources opens up possibilities for structural studies of a wide range of biomolecules in gas-phase. Ion mobility spectrometry coupled with mass spectrometry has seen spectacular growth for the last two decades. The introduction of ion mobility to ion spectroscopy has been of growing attention in recent years. While IMS can be used as an isomer filter to reduce spectral congestion of ions, highly resolved ion spectra facilitates the identification of the isomer peaks separated by IMS. Taken together, spectra and collision cross section (CCS) measurements on the same system prove to be rather useful for structural analysis and provide clearer spectroscopic fingerprint to isomers. Therefore, this paper briefly summarized advances in ion spectroscopy research based on ion mobility-mass spectrometry technology, introduced representative researches, and prospected possible development in multi-dimensional structural mass spectrometry. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of long non-coding RNA cancer susceptibility candidate 9 (CASC9) on the proliferation and apoptosis of colorectal cancer cells, and to explore the relationship between its mechanism and miR-195-5p cyclin D1 (CCND1) axis. Methods The expression levels of CASC9 in the normal colon epithelial cell line NCM460 and colorectal cancer cell lines SW480, SW620 and HCT-116 were detected by real-time fluorescence quantitative PCR. Colorectal cancer cells with the highest expression of CASC9 were divided into the CASC9 interference group and control group. The cells in the CASC9 interference group were transfected with SHRNA-CASC9, and the control group with SH-NC. Cell proliferation of the two groups was detected by CCK-8, apoptosis rates of the two groups were detected by flow cytometry, and mRNA expression levels of miR-195-5p and CCND1 were detected by real-time quantitative PCR. The potential binding sites of CASC9 and miR-195-5p, and miR-195-5p and CCND1 were predicted by StarBase database, and were verified by double luciflucase reporter assay. Results Compared with NCM460 cells, the expression levels of CASC9 in SW480, SW620 and HCT-116 cells increased, and the expression level of CASC9 in SW480 cells was higher than those in SW620 and HCT-116 cells (all P<0. 01). SW480 cells were selected for subsequent experiments. Compared with the control group, the proliferation ability of CASC9 cells decreased (P<0. 01), the apoptosis rate increased (P< 0. 05), and the expression levels of miR-195-5p and CCND1 mRNA increased in the interference group (both P<0. 01). StarBase database analysis showed that CASC9 could target and adsorb miR-195-5p. CCND1 was the downstream target protein of miR-195-5p. Luciferase reporter gene results showed that CASC9 could specifically bind with miR-195-5p (P< 0. 01). Conclusion CASC9 is highly expressed in colorectal cancer tissues and cells, and its mechanism may be that CASC9, as a miR-195-5p adsorption sponge, regulates the expression of CCND1 and promotes the proliferation of colorectal cancer cells and thus inhibits apoptosis. [ABSTRACT FROM AUTHOR]

Objectives Meta-analysis was used to evaluate the seroprevalence of Anaplasmaphagocytophilumin Chinese mainland popupation， in order to provide a reference for the prevention and treatment of human granulocytic anaplasmosis. Methods Computer-researched literature on the seroprevalence of Anaplasmaphagocytophilum in Chinese mainland popupation was searched by Chinese and English databases -CNKI， VIP databases， WanFang Data and PubMed. First we determined the literature's inclusion and exclusion criteria，selected the literature and extracted relevant data，and recorded data in an Excel spreadsheet. And Rstudio software was used to perform meta-analysis of the seropositivity rate of A. phagocytophilum among Chinese mainland popupation，as well as subgroup analysis of gender，population characteristics and age groups. Results A total of 20 articles were included in the study. After calculation， the combined seroprevalence of A. phagocytophilum in mainland China was 17% (95%CI 0.11-0.23)，and the positive rate after male combination was 15% (95% CI 0.02-0.39)， the women's A. phagocytophilum positive rate was 10% (95% CI 0.04-0.17)，P＜0.01； The seropositivity rate of healthy population data was 13% (95%CI 0.08-0.20) and the seropositivity rate of high-risk data group was 20% (95%CI 0.10-0.32)，P＜0.01. The positive rate of the age group ≤54 was 24% (95%CI 0.11-0.40)，age group 55-69 was 24% (95%CI 0.06-0.49)， age group ≥70 was 22% (95%CI 0.07-0.42)，P＜0.01. 20 studies were tested as not publication biased．Conclusion The total positive rate of A. phagocytophilum in Chinese mainland population is not high， but it has a potential increasing trend. It is worth making attention to the majority of medical workers in order to prevent and treat it early. According to this meta-analysis， gender and population characteristics may be factors influencing the seroprevalence of A. phagocytophilum in Chinese mainland population. [ABSTRACT FROM AUTHOR]