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Objective To investigate changes of serum Angptl2 and Angptl4 levels in patients with polycystic ovary syndrome (PCOS) and their relationship with ovarian interstitial hemodynamics. Methods A total of 123 PCOS patients (the PCOS group) and 41 healthy volunteers (the control group) were selected. The serum Angptl2 and Angptl4 levels, the peak systolic velocity (PSV), pulsation index (PI) and resistance index (RI) of ovarian interstitial were compared between the two groups. The relationship between Angptl2, Angptl4, PSV, PI, RI and PCOS and their predictive value to PCOS were analyzed. Results The PI and RI values of left and right ovarian interstitial were lower in the PCOS group than those in the control group, and PSV values were higher than those in the control group (P＜0.05). Pearson analysis showed that serum Angptl2 and Angptl4 were positively correlated with PSV of left and right ovarian interstitial, and negatively correlated with PI and RI of left and right ovarian interstitial in patients with PCOS (P＜0.01). Logistic regression results showed that high HOMA-IR (OR=1.921, 95%CI: 1.017-4.154), high BMI (OR=1.459, 95%CI: 1.085-3.220), high Angptl2 (OR=2.625, 95%CI: 1.330-6.324), high Angptl4 (OR=3.543, 95%CI: 1.915-8.147) levels were related factors affecting the occurrence of PCOS (P＜0.05). Receiver operating characteristic curve (ROC) showed that the AUC (95%CI) of predicted PCOS were 0.747 (0.661-0.821) and 0.769 (0.685-0.841) when serum Angptl2 and Angptl4 were used alone. It was lower than that of 0.879 (0.793-0.921, P＜0.05) for the combined application. Conclusion The abnormal levels of serum Angptl2 and Angptl4 in PCOS patients are related to the hemodynamics of ovarian interstitial, and they are closely related to the occurrence of PCOS, which can be used as reference indexes for the evaluation of PCOS. [ABSTRACT FROM AUTHOR]

AIM: To investigate whether Panax notoginseng saponins (PNS) attenuate arecoline (ANE)-induced human oral submucous fibrosis (OSF) by regulating ferroptosis, and to study its mechanism. METHODS: The OSF model was established by treating human immortalized keratinocyte cell line HaCaT with ANE. There were 6 groups created: blank group, model group(50 mg/L ANE), low-, medium- and high-dose (12. 5, 25 and 50 mg/L)PNS groups, and liproxstatin-1 (ferroptosis inhibitor) group. The morphological changes of the cells were observed under a light microscope. Cell viabilty was detected by CCK-8 assay. Collagen type I (Col-l), solute carrier family 7 member 11(SLC7A11) and glutathione peroxidase 4(GPX4)protein expression levels were detected by Western blot. The morphological changes of mitochondria were observed by transmission electron microscopy. The Fe2+ level was detected by FerroOrange fluorescent probe. The intracellular reactive oxygen species (ROS) content was detected by DCFH-DA fluorescent probe. The content of glutathione (GSH)was determined by colorimetry. RESULTS: Compared with blank group, the majority of the cells in model group displayed long spindle shape, fibrotic appearance, unstable adhesion, and obvious morphological changes. Under electron microscope, mitochondrial double membrane density increased, and cristae decreased significantly, presenting typical ferroptosis. The expression of Col-I increased, GPX4 and SLC7A11 protein expression decreased, Fe2+ and ROS levels increased, and GSH content decreased (all/><0. 05). Compared with model group, most of the cells in low-, medium- and high-dose PNS groups were round and epit he lo id, with decreased mitochondrial membrane density, increased ridge number and normal morphology, decreased Col-I expression and ROS level, and increased GSH content (all P<0. 05). Compared with model group, the protein expression levels of GPX4 and SLC7A11 in medium- and high-dose PNS groups were significantly increased, while the content of Fe2+ was significantly decreased (all P<0. 05). There were no significant differences in cell and mitochondrial morphology and indicator levels between liproxstatin-1 group and high-dose PNS group (all 05). CONCLUSION: Panax notoginseng saponins can alleviate OSF by preventing the ferroptosis of HaCaT cells induced by ANE. [ABSTRACT FROM AUTHOR]

BACKGROUND: Previous studies have shown that a single change in the expression of a gene or the state of a cell in the area of spinal cord injury has no significant effect on functional recovery after spinal cord injury. An increasing number of evidence shows that the regulation of cellular microenvironment after spinal cord injury is a key factor hindering the recovery of neurological function. OBJECTIVE: To review the biological characteristics of cellular microenvironment before and after spinal cord injury, including the effect and mechanism of mutual regulation among different kinds of cells and extracellular components on nerve repair after spinal cord injury. METHODS: The first author retrieved the databases including PubMed and Web of Science for articles published from January 2000 to December 2021 using the key words of “spinal cord injury, glial cell, neuron, immune cell, neural stem cell, extracellular matrix, cytokine, extracellular vesicle, regeneration”. Finally, 64 articles were selected for analysis. RESULTS AND CONCLUSION: (1) After spinal cord injury, among the cellular components of the cellular microenvironment, the interaction among glial cells, which account for the highest proportion, and the interaction with neurons are particularly critical. (2) Among the extracellular components after spinal cord injury, the hydrogels with good biocompatibility used to imitate natural extracellular matrix can effectively simulate and reconstruct the cellular microenvironment in the injured area and promote axonal elongation. (3) Among the extracellular regulatory factors after spinal cord injury, proinflammatory factors such as tumor necrosis factor-α and interleukin-1β aggravate the inflammatory storm of cellular microenvironment. Using receptor inhibitors or blocking related pathways to down-regulate the expression of adverse extracellular regulatory factors is an effective therapeutic method in the cellular microenvironment. At the same time, there are also studies on increasing the expression of anti-inflammatory factors such as interleukin-10 in the microenvironment of the spinal cord and on inhibiting the development of inflammation in the injured area. (4) Recently, extracellular vesicles have also played an important role in the cellular microenvironment as carriers of information. (5) This article reveals multiple sets of mutual regulatory relationships between cellular components and extracellular components in the cellular microenvironment after spinal cord injury, emphasizing that the neuroreparative effects of various components in the cellular microenvironment are not isolated. [ABSTRACT FROM AUTHOR]

The CCS Hydropower Station in Ecuador is characterized by the axis of the diversion to power generation system up to 2 800 m long, the internal and external heads more than 600m, the large groundwater recharge, the developed steeply dipping broken zone and the densely caves criss-crossly arranged, all of these result in gushing water problems during construction and many key and difficult points in waterproof-drainage design. According to the sensitivity analysis of internal water leakage based on seepage field's analysis over various conditions by FLAC 3D, multi-factor instability analysis for wedges considering joints' and fractures' water pressure coupling of in-situ stress by 3DEC, combining with the high-pressure permeability characteristics analysis based on high water pressure tests results, features analysis of the safety monitoring data at the characteristic times after the water-filling test until recently, the research on key technologies for waterproof-drainage and also certification for control measures were finally put forward. Using water-permeable lining, combining with geomembrane, consolidation grouting, curtain grouting for the upstream high-head water diversion system, drainage hole curtain based on drainage gallery and system drainage holes for the downstream power generation system and so on, after all these permanent and temporary combination measures, the power station currently is running well, the safety monitoring data are not anomaly and have been converged for a long time, which certifies the rationality of the waterproof-drainage design for diversion to power generation system under the high internal and external water condition. [ABSTRACT FROM AUTHOR]

The paper discussed classification limitation and computational complexity of categorization algorithm based on sparse representation, and proposed a novel weighted locally linear KNN algorithm for text categorization, which used weighted function to make the representation coefficients sparse, and introduced nonnegative constraints to improve the classification performances. Moreover, this paper gave the parameters experiments to select the optimization value, and gave the theoretical proof of the algorithm. The experiments show the superiority of the algorithm based on weighted locally linear KNN, which has an average improvement on 2. 49% and 0. 85% in precision and recall, compared with the traditional mcxlel. It means the text categorization algorithm based on weighted locally linear KNN has advantages of high classification accuracy and strong convergence, which is suitable for high-dimensional data classification. [ABSTRACT FROM AUTHOR]

BACKGROUND: The human leukocyte antigen (HLA) has undergone long-term evolution to form diverse polymorphisms. In recent years, due to the increase in the number of examinees and the rapid development of HLA typing technology, novel HLA alleles have been discovered constantly. OBJECTIVE: To analyze the full-length sequence and 18 point mutations of HLA-B gene in a leukemia patient and her family using the next-generation sequencing technology. METHODS: Polymerase chain reaction and sequence-specific oligonucleotide probes (PCR-SSOP) and polymerase chain reaction-sequence based typing (PCR-SBT) revealed abnormalities in the patient’s HLA-B. To identify the genotype, we sequenced the full length of the gene by next-generation sequencing technology and collected blood samples from the patient’s father, mother and two sisters for genetic analysis of HLA genes. RESULTS AND CONCLUSION: Both PCR-SSOP and PCR-SBT indicated that the HLA-B sample had no perfectly matched genotype. Further detection using the next-generation sequencing technology revealed that the novel allele had 18 base mutations in the exon, intron and 3'UTR compared to the most homologous allele B*15:09:01. Five exon base mutations were located in the exons 3 and 4, which were: 486G→C, 583T→C, 636T→C, 652A→G, 756C→T, resulting in changes in the five corresponding codons, including 171 tyrosine (Tyr) → histidine (His) and 194 isoleucine (Ile) → valine (Val). A pedigree survey found that the patient’s novel HLA B allele was inherited from her father. The novel allele sequence was submitted to the Genbank database (MG595995). A novel HLA-B allele was confirmed by the next-generation sequencing, which was officially named HLA-B*15:435 by the World Health Organization HLA Factor Nomenclature Committee in December 2017. [ABSTRACT FROM AUTHOR]

The underground powerhouse system of CCS hydropower station in Ecuador is characterized by large-span caverns and three directions short distance in space, which brings about complicated three directions thin pillar problems. The joints development and strike unfavorable in the caverns area, it is easy to form unstable wedge. Based on the European and American codes, using domestic and foreign engineering analogy method, two-dimensional and three-dimensional analysis methods, and based on the “NATM”, the design ideas of integrally using shotcrete with wire mesh and bolts, partially considering shotcrete with steel set and bolts for supporting, and the design concept of partial reinforcement for wedges on the basis of integral system support were finally put forward. This project had avoided the delayed construction period by large-scale pre-stressed anchors and cables. At present, the power station is running well, the safety monitoring data are not anomaly and have been converged for a long time, which certifies the rationality of the support structures for underground caverns. [ABSTRACT FROM AUTHOR]

The area of CCS Hydropower Station tailrace channel belongs to high earthquake degree area, where the geological condition is poor, overburden is deep, the elevation of bedrock surface varies greatly and the tailrace channel is very closely to the only major road of the power station, directly leading to the limitation of excavation range of tailrace channel and forming a stability issue for high and steep slope condition. This project included multiple difficult points and complex conditions, which was a typical case of slope design. In order to make sure safety, “multiple parameter truth value inversion analysis” was adopted to obtain geological parameter, designer put forward the combined supporting design idea of ground wall, pre-stress cable, top beam anti-slide pile, pre-stress rock bolt and grout bolt. This project guaranteed the road safety and avoided the delayed construction period by other road plan. At present, the power station is running well, the safety monitoring data are not anomaly and have been converged for a long time, which certifies the rationality of design idea and support plan for tailrace channel slope. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of "U" suture hemostasis on the amount of bleeding in cesarean section of placenta previa patients. Methods: 96 cases of patients undergoing placenta previa cesarean section maternal admitted from September 2015 to September 2017 in our hospital were selected as the research materials and randomly assigned to two groups, with 48 cases in two groups. Both groups of patients were given the routine hemostatic treatment, the control group was given "8" suture hemostasis, the observation group was given "U" suture hemostasis, the index and hemostatic effect were compared between two groups. Results: The operation time, hemostasis time, and intraoperative blood loss of observation group were all significantly less(shorter) than those of the control group(P<0.05), the thrombin time(TT), plasma thrombin reduction time(PT), D-dimer(DD), platelet(PLT), and activated partial clotting activase time(APTT) were significantly lower than those of the control group(P<0.05), the levels of hemoglobin(HGB) and fibrinogen(FIB) were significantly higher than those of the control group(P<0.05), the effective rate of hemostasis[97.92%(47/48)] was significantly higher than that in the control group[(85.42%(41/48)](P<0.05), the time of ambulation and hospitalization were significantly shorter than those of the control group(P <0.05). Conclusion: Implementation of "U" suture hemostasis has the advantages of simple operation in the rapid hemostasis of placenta previa cesarean section, it could reduce the amount of bleeding and ensure maternal health with high safety. [ABSTRACT FROM AUTHOR]

For the embedded water stop, the position conflicts between the waterstop and the rebar appear frequently in design and construction. For the back attached waterstop, the exposure outside can cause harm for the durability. In order to solve the inadaptability, according to the structural characteristics and the working principles of those two kinds of waterstops, the side attached waterstop was initially proposed and firstly used in Lanzhou Water Source Project with good adaptability. The side attached waterstop has provided convenient both for the design and construction. [ABSTRACT FROM AUTHOR]

Objective To study the influence of tarsal gland massage on postoperative curative effect of femtosecond laser combined with excimer laser keratomileusis. Methods A prospective study was conducted on patients who underwent the same surgical approach by the same surgeon. 78 eligible patients were randomly divided into two groups: 39 patients in experimental group, equal to 78 eyes, were given tarsal gland massage before the operation; and the other 39 patients in control group, equal to 78 eyes, were not given tarsal gland massage before the operation. The presence condition of postoperative interlayer eyelid fat of the two groups of patients was investigated. After that, the patients were divided into two groups according to above eyelid fat condition: with eyelid fat group and without eyelid fat group, and the visual acuity recovery status were compared between the two groups.Results In the control group after the operation, 3 patients could not be contacted in the follow-up study, but a total of 36 patients, equal to 72 eyes were observed by slit lamp, and there were totally 10 eyes with interlayer eyelid fat, accounting for 13.89%; Whereas, 2 patients could not be contacted in the experimental group, but a total of 37 patients, equal to 74 eyes were observed, and there were only 2 eyes with interlayer eyelid fat,accounting for 2.7%. The P value of comparison between these two groups was less than 0.01, which had statistical differences. There was significant difference between the two groups(P <0.05) in the recovery of visual acuity within one month after the operation. Conclusion Tarsal gland massage can effectively reduce the residual of interlayer eyelid fat after the operation of femtosecond laser combined with excimer laser keratomileusis, thereby it can avoid the delay of vision recovery and effectively improve the postoperative curative effect. [ABSTRACT FROM AUTHOR]

目的探讨系统性红斑狼疮(SLE)患者血清血小板反应蛋白1(TSP-1)水平变化及临床意义。方法选择SLE患者97例作为观察组(活动期53例、缓解期44例)、体检健康志愿者50例作为对照组。用酶联免疫吸附法检测两组血清TSP-1水平,并检测血红蛋白、白细胞、血小板、尿素氮、血肌酐、24 h尿蛋白、补体C3、补体C4、抗ds DNA抗体水平。用Pearson相关分析SLE患者血清TSP-1水平与其他指标的相关性。结果观察组血清TSP-1水平高于对照组(P <0. 05),而血红蛋白、白细胞、血小板、补体C3、补体C4水平低于对照组(P均<0. 05),活动期SLE患者血清TSP-1水平较缓解期高(P <0. 05)。合并口腔溃疡、皮疹、脱发、关节炎、狼疮性肾炎、溶血性贫血、白细胞减少、血小板减少、低补体C4和低补体C3的SLE患者血清TSP-1水平高于未合并患者(P均<0. 05)。SLE患者血清TSP-1水平与血红蛋白、白细胞、血小板、补体C3和补体C4水平呈负相关(r分别为-0. 725、-0. 697、-0. 716、-0. 731、-0. 709,P均<0. 05),与抗ds DNA抗体水平呈正相关(r=0. 752,P <0. 05),而与尿素氮、血肌酐、24 h尿蛋白无相关性(r分别为0. 235、0. 347、0. 193,P均> 0. 05)。结论 SLE患者血清TSPS-1水平升高,其水平越高患者病情越严重,并发症越多。 [ABSTRACT FROM AUTHOR]

Objective: To compare the efficacy of domperidone and low-dose Erythromycin in the treatment of feeding intolerance in very low birth weight infant （VLBWI）Methods: A retrospective analysis was performed on 90 cases of VLBWI with feeding intolerance who admitted in our hospital from January 2011 to December 2013. According to the choice of therapy, the patients were divided into group A (domperidone group, n=42) and group B (low-dose erythromycin group, n=48). On the basis of comprehensive treatment the children of different groups were treated with different drugs as mentioned above the feeding intolerance symptoms.then the subside time of feeding intolerance symptoms, time to regain birth weight, the incidence of total parenteral feeding time, duration of hospitalization and incidence of side effects were observe and compared between two groups. Results: After treatment，all the children in both groups，achieved feeding intolerance symptoms, regained birth weight, total parenteral nutrition in the discharge standards. No statistically significant difference was found in the time of indicators mentioned above between two groups (P>0.05). No related adverse reactions was adversed in group A，while six cases of local pain caused by intravenous erythromycin was adversed in group B，the incidence rate was 12.5%, it was significantly higher than that of group A (P <0.05). Conclusion: Both domperidone and low-dose intravenous erythromycin could be effective and equal in the treatment of feeding intolerance in VLBWI. But domperidone might have higher security. [ABSTRACT FROM AUTHOR]

BACKGROUND: The role of miR-29a and aquaporin-4 in astrocyte injury has been demonstrated. However, the underlying mechanisms remain largely unknown. OBJECTIVE: To investigate the effects of the regulation of aquaporin-4 by miR-29a on hydrogen peroxide-induced astrocyte injury. METHODS: (1) Primary cultured mouse astrocytes were used to study the damage response of mouse astrocytes induced by different concentrations of hydrogen peroxide, and the cells were divided into control, hydrogen peroxide, hydrogen peroxide + empty vector, and hydrogen peroxide + miR-29a overexpression groups. The survival rate of mouse astrocytes was detected by MTT assay. The protein expression of apoptosis-related proteins (Bcl-2, Bax, and cleaved-Caspase-3), aquaporin-4 and glial fibrillary acidic protein was detected by western blotting. The expression of miR-29a in mouse astrocytes was detected by quantitative reverse transcription-polymerase chain reaction. Mouse astrocyte apoptosis was detected by flow cytometry. (2) Twelve female BALB/c mice were randomly divided into empty vector and miR-29a overexpression groups with six mice in each group. A T10 spinal cord contusion injury mouse model was established, and the injection of recombinant lentiviral vectors (empty and miR-29a overexpression vectors) was administered after modeling. The expression of aquaporin-4 and glial fibrillary acidic protein at the spinal cord injury site in mice was detected by immunohistochemical staining at 7 days after surgery. RESULTS AND CONCLUSION: (1) The activity of astrocytes and the expression of anti-apoptosis protein Bcl-2 were decreased, while the expression of proapoptosis proteins Bax and cleaved-caspase-3 was increased in a concentration-dependent manner after hydrogen peroxide-induced injury. In mouse astrocytes with hydrogen peroxide-induced injury, miR-29a expression was decreased, while protein expression of aquaporin-4 and glial fibrillary acidic protein was significantly increased; overexpression of miR-29a inhibited protein expression of aquaporin-4 and apoptosis. Aquaporin-4 may be a downstream target gene of miR-29a as predicted using informatics software. (2) Overexpression of miR-29a at the injury site inhibited the protein expression of aquaporin 4 and glial fibrillary acidic protein in the spinal cord contusion injury mouse model compared with that of the empty vector injection group. (3) These findings suggest that the regulation of aquaporin 4 by miR-29a can inhibit hydrogen peroxide-induced astrocyte apoptosis. [ABSTRACT FROM AUTHOR]

The penstocks of CCS Hydropower Station in Ecuador are characterized by deep buried depth and high waterhead. The conventional lining design leads to much reinforcement. The permeable lining is adopted in the CCS penstock. The coupling analysis of the seepage field and the finite element structure of the concrete lining was carried out and the permeable lining reinforcement was calculated. The result shows that the lining concrete can meet the stress requirements under the condition of completion and maintenance. For operating condition, the reinforcement needed by the permeable lining is quite small. For water hammer condition, the reinforcement needed by the permeable lining is quite large compared with the operating condition. The permeable lining is suitable in practice, and it can greatly reduce the amount of reinforcement compared with the conventional lining. [ABSTRACT FROM AUTHOR]

Objectives To investigate the infection of H.pylori in 528 children with epigastric pain in Dali Bai Autonomous Prefecture People’ s Hospital, to study the correlation between infection and iron deficiency anemia (IDA) and to provide evidence for the diagnosis and treatment of H.pylori infection and IDA in children. Method A total of 528 children aged 3 to 13 who received 13C-UBT due to upper abdominal pain in the outpatient department of Dali Bai Autonomous Prefecture People’s Hospital from January 2019 to December 2020 were selected, and the gender and ethnicity of the detected children were analyzed by questionnaire. There were 119 H.pylori positive children listed as the observation group, and 409 H.pylori negative children listed as the control group. Hemoglobin (Hb), mean red blood cell Volume (MCV), serum iron (SI), total iron binding capacity (TIBC) and serum ferritin (SF) were tested to determine the occurrence of IDA, and the correlation between H.pylori infection and IDA was analyzed. Results The total infection rate of H.pylori in 528 children was 22.53%, and the positive rates of H.pylori in male and female children were 22.88% and 22.22%, respectively. There was no significant difference in the positive rate of H.pylori in male and female children (χ2 = 0.08, P > 0.05). The positive rates of H.pylori infection in Han, Bai, Tibetan and other minority children were 17.95%, 20.69%, 64.29% and 26.92%, respectively. The positive rate of H.pylori infection was significantly different (χ2 = 273.659, P < 0.01). The levels of Hb, MCV, SI and SF in the observation group were significantly lower than those in the control group, and the level of TIBC was significantly higher than that in the control group, with statistical significance (P < 0.05). Conclusion H.pylori infection rate was not related to gender, and there were obvious ethnic clusters. H.pylori infection is associated with IDA in children and may be a risk factor for IDA. Therefore, routine H pylori monitoring and anti-H.pylori treatment should be included in the clinical treatment of IDA to obtain better clinical outcome. [ABSTRACT FROM AUTHOR]

BACKGROUND: The special genetic law of short tandem repeat on chromosome X (X-STR) makes it incomparable with autosome markers in forensic identification. However, the population genetics data is far less than that of the autosome STR, and especially the haplotype data are rarely reported. OBJECTIVE: To study the genetic polymorphism of 12 X-STR loci in Shenzhen area by pedigree analysis, aiming to provide scientific and effective data for the application of X-STR in forensic medicine and genetics. METHODS: The blood samples of 118 families were taken to extract DNA by Chelex-100, followed by PCR amplification using Investigator Argus X-12 kit. The frequency of alleles of 231 unrelated individuals was counted by direct counting method and Excel software. Hardy-Weinberg equilibrium test was performed on 12 X-STR loci of female samples by chi-square test. Discrimination power and mean exclusion chance were calculated according to the formula. Pedigree analysis was done to identify haplotypes of female samples and the haplotype frequencies of 4 linkage groups in 111 fathers and 119 mothers were calculated using direct counting method and Excel software. RESULTS AND CONCLUSION: In this study, 349 haplotypes were obtained. There were 238, 139, 153 and 157 haplotypes in linkage groups X1-X4, respectively. The polymorphism of DXS10135 locus was the highest with 21 alleles, while the polymorphism of DXS7423 locus was the worst with only 4 alleles. The combined discrimination power was 0.99999999 in males and 0.99999999 in females. The combined mean exclusion chance was 0.99999999 in trio cases, and 0.99999811 in duo cases. These findings indicate that the X-12 detection system has high polymorphism in Shenzhen Han population, and has important application value in forensic individual identification and paternity testing. [ABSTRACT FROM AUTHOR]

BACKGROUND: High resolution validation of human leukocyte antigen (HLA) is one of the essential step before hematopoietic stem cell transplantation. With the continuous development of the work and the number of people tested continues to increase, human leukocyte antigen new alleles are constantly emerging. Due to the late discovery of some new genes and the lack of relevant reports, the gene frequency cannot be accurately calculated. In the absence of relevant data, it is easy to misjudge the typing results. The result of mismatch affects the success rate of transplantation. OBJECTIVE: An allele of HLA-A*24:353 was detected and confirmed in HLA, and its occurrence frequency was analyzed. METHODS: Polymerase chain reaction-sequencing-based typing method was used to detect five loci of HLA- A, B, C, DRB1, and DQB1 in 543 clinical transplantation matching recipients from September 2019 to May 2020. Next-generation sequencing was used to determine the full-length sequence of HLA-A*24:02/24:353 ambiguous results to obtain high resolution typing results of HLA and calculate the occurrence frequency of HLA-a *24:353 gene. RESULTS AND CONCLUSION: The ambiguous results of HLA-A*24:02/24:353 were detected in 146 cases. After further confirmatory tests using next-generation sequencing, the results of HLA-A*24:353 gene were detected in 5 cases (3.42%) in A*24 group and 0.92% in 543 cases. The results suggest that HLA-A*24:353 may not be rare allele in China. When HLA-A*24:02/24:353 ambiguous results appear in clinical transplantation matching recipients for high resolution typing, further confirmation is needed to increase the accuracy of typing results and improve the success rate of transplantation matching. [ABSTRACT FROM AUTHOR]

BACKGROUND: Due to the polymorphism of HLA, a large number of ambiguities have been generated by conventional HLA typing techniques, and confirmed stereotypes of ambiguous results based on group-specific haploid full-length typing are rarely reported. OBJECTIVE: To analyze the accuracy of HLA-typing ambigulity based on group-specific haploid full-length sequencing. METHODS: The low-resolution results were used as the starting point for two ambiguous samples. Sanger sequencing (PCR-SBT) based on haploid full-length was performed after group-specific amplification. RESULTS AND CONCLUSION: One case showed a new A*02:03:01 allele, which was found a mutation in NT817 from C to T in comparison with A*11:01:01:01. The other case indicated another new C*07:02:01:01, which was found a mutation in NT879 from A to G in comparison with C*08:01:01. In conclusion, these results indicate that the group-specific haploid full-length sequencing method can be used to accurately classify HLA alleles and to discover new alleles. [ABSTRACT FROM AUTHOR]