1. Adsorption of Cholera and Heat-Labile Escherichia coli Enterotoxins by Various Adsorbents: an In Vitro Study
- Author
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A.B. Sarr, Timothy D. Phillips, Gerald N. Woode, K.J Clark, and J. Ramu
- Subjects
inorganic chemicals ,Boehmite ,Chemistry ,Cholera toxin ,Enterotoxin ,medicine.disease_cause ,Microbiology ,Adsorption ,Biochemistry ,Casein ,visual_art ,Bentonite ,medicine ,visual_art.visual_art_medium ,Charcoal ,Escherichia coli ,Food Science ,Nuclear chemistry - Abstract
A variety of common inorganic adsorbents representing aluminas, zeolites, phyllosilicate clays, silica, and carbon were compared for their abilities to adsorb cholera toxin (CT) and heat-labile (LT) Escherichia coli enterotoxin. An appropriate assay system for the enterotoxins was developed using the Y-1 mouse-adrenal-tumor cell line, End points were determined by counting the number of rounded (cytotonic) cells at the relevant dilution. The adsorption varied between 177.0 × 106 and 109.6 × 102 CYTU (cytotonic titer unit) for CT with charcoal and boehmite respectively, and between 60.7 × 104 and 180.4 × 101 CYTU for LT with charcoal and boehmite respectively. Several of the other materials adsorbed CT and LT well, particularly attapulgite and sodium bentonite. The tightness of CT and LT binding to sodium bentonite and charcoal was determined by washing the adsorbent-enterotoxin pellets. Both toxins were strongly adsorbed, with dissociation of only 46.3 × 10° CYTU (
- Published
- 2019