12 results on '"Alfredo Manzano-García"'
Search Results
2. A Latin American consensus meeting on the essentials of mixed pain
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Mariano Fernandez-Fairen, Carlos Alberto Calderón-Ospina, Juythel Chen, Manuel Duarte Vega, Freddy Fernández-Villacorta, Felipe Gómez-García, Leonardo López-Almejo, Alfredo Manzano-García, Eduardo Hernández-Méndez Villamil, Camilo Partezani Helito, Delia Ruiz-Rodríguez, Guillermo Salas-Morales, Alfredo Servin-Caamaño, Argelia Lara-Solares, Marcelo Puello-Vales, and Grisell Vargas-Schaffer
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General Medicine - Published
- 2023
3. Effect of local infiltration with oxytocin on hemodynamic response to surgical incision and postoperative pain in patients having open laparoscopic surgery under general anesthesia
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Héctor Zayas-González, Abimael González-Hernández, Donaciano Hernández‐Rivero, Alfredo Manzano-García, Miguel Condés-Lara, Sergio Flores‐Fierro, Guadalupe Martínez-Lorenzana, Marco Antonio García‐Cuevas, Liliana Vaca‐Aguirre, and Juan Carlos Granados‐Mortera
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Adult ,Male ,Laparoscopic surgery ,Lidocaine ,Visual analogue scale ,medicine.medical_treatment ,Surgical Wound ,Analgesic ,Hemodynamics ,Anesthesia, General ,Oxytocin ,Random Allocation ,03 medical and health sciences ,0302 clinical medicine ,Humans ,Medicine ,030212 general & internal medicine ,Anesthetics, Local ,Laparoscopy ,Pain Measurement ,Pain, Postoperative ,medicine.diagnostic_test ,business.industry ,Perioperative ,Middle Aged ,Anesthesiology and Pain Medicine ,Anesthesia ,Female ,business ,Surgical incision ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Background Preemptive analgesia encompasses different perioperative interventions that have the final aim of decreasing postoperative pain and improving recovery. Recently, peripheral analgesic effects of oxytocinergic modulation have been suggested. In this regard, we tested the potential analgesic effects of subcutaneous oxytocin (OT) infiltration in patients submitted to laparoscopic cholecystectomy. Methods Thirty patients with similar general characteristics and medical physical conditions were evaluated. The patients were assigned by simple random selection to one of three groups: (a) OT group (n = 10), which received preincisional subcutaneous OT (4 µg/4 ml saline) in the surgical sites for trocar placements; (b) Lidocaine group (n = 10), which received subcutaneous lidocaine 1% (4 ml) in the surgical sites; and (c) Control group (n = 10), which did not receive any treatment. Then we measured the effect of those treatments on the hemodynamic variations produced as responses to the surgical incisions and trocar insertions (open port placements using the Hasson technique). Moreover, we assessed the intensity of postoperative pain with the visual analogue scale during recovery and 24 hr after surgery. Results Hemodynamic parameters were stable in both intervention groups (subcutaneous OT and lidocaine) during the surgical incisions and trocar placements, whereas a most likely sympathetic activation due to trocar insertions (open port placements) was not blunted in the placebo group. Furthermore, postoperative pain was diminished in both OT and lidocaine groups when compared to the control group. Conclusions Preincisional subcutaneous OT administration reduced the hemodynamic response produced by the latter. Furthermore, OT also diminished postoperative pain.
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- 2019
4. The Rostral Agranular Insular Cortex, a New Site of Oxytocin to Induce Antinociception
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Guadalupe Martínez-Lorenzana, Abimael González-Hernández, Alfredo Manzano-García, Miguel Condés-Lara, Antonio Espinosa de los Monteros-Zúñiga, and Mohammed Gamal-Eltrabily
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0301 basic medicine ,Male ,Nociception ,endocrine system ,Neuropeptide ,Insular cortex ,Oxytocin ,03 medical and health sciences ,0302 clinical medicine ,Formaldehyde ,Neural Pathways ,medicine ,Animals ,GABAergic Neurons ,Rats, Wistar ,Research Articles ,Cerebral Cortex ,Inflammation ,Neurons ,Chemistry ,General Neuroscience ,Bicuculline ,Oxytocin receptor ,030104 developmental biology ,nervous system ,Hypothalamus ,GABAergic ,Neuroscience ,030217 neurology & neurosurgery ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug ,Paraventricular Hypothalamic Nucleus - Abstract
The rostral agranular insular cortex (RAIC) is a relevant structure in nociception. Indeed, recruitment of GABAergic activity in RAIC promotes the disinhibition of the locus ceruleus, which in turn inhibits (by noradrenergic action) the peripheral nociceptive input at the spinal cord level. In this regard, at the cortical level, oxytocin can modulate the GABAergic transmission; consequently, an interaction modulating nociception could exist between oxytocin and GABA at RAIC. Here, we tested in male Wistar rats the effect of oxytocin microinjection into RAIC during an inflammatory (by subcutaneous peripheral injection of formalin) nociceptive input. Oxytocin microinjection produces a diminution of (1) flinches induced by formalin and (2) spontaneous firing of spinal wide dynamic range cells. The above antinociceptive effect was abolished by microinjection (at RAIC) of the following: (1) L-368899 (an oxytocin receptor [OTR] antagonist) or by (2) bicuculline (a preferent GABA(A) receptor blocker), suggesting a GABAergic activation induced by OTR. Since intrathecal injection of an α(2A)-adrenoceptor antagonist (BRL 44408) partially reversed the oxytocin effect, a descending noradrenergic antinociception is suggested. Further, injection of L-368899 per se induces a pronociceptive behavioral effect, suggesting a tonic endogenous oxytocin release during inflammatory nociceptive input. Accordingly, we found bilateral projections from the paraventricular nucleus of the hypothalamus (PVN) to RAIC. Some of the PVN-projecting cells are oxytocinergic and destinate GABAergic and OTR-expressing cells inside RAIC. Aside from the direct anatomic link between PVN and RAIC, our findings provide evidence about the role of oxytocinergic mechanisms modulating the pain process at the RAIC level. SIGNIFICANCE STATEMENT Oxytocin is a neuropeptide involved in several functions ranging from lactation to social attachment. Over the years, the role of this molecule in pain processing has emerged, showing that, at the spinal level, oxytocin blocks pain transmission. The present work suggests that oxytocin also modulates pain at the cortical insular level by favoring cortical GABAergic transmission and activating descending spinal noradrenergic mechanisms. Indeed, we show that the paraventricular hypothalamicnucleus sends direct oxytocinergic projections to the rostral agranular insular cortex on GABAergic and oxytocin receptor-expressing neurons. Together, our data support the notion that the oxytocinergic system could act as an orchestrator of pain modulation.
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- 2020
5. The role of peripheral vasopressin 1A and oxytocin receptors on the subcutaneous vasopressin antinociceptive effects
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Alfredo Manzano-García, Miguel Condés-Lara, Abimael González-Hernández, Guadalupe Martínez-Lorenzana, and Irma A. Tello-García
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Male ,Nociception ,0301 basic medicine ,Receptors, Vasopressin ,endocrine system ,medicine.medical_specialty ,Vasopressin ,Indoles ,Pyrrolidines ,Vasopressins ,Injections, Subcutaneous ,Nerve Fibers, Myelinated ,Piperazines ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Premovement neuronal activity ,Receptor ,Evoked Potentials ,Pain Measurement ,Vasopressin receptor ,Analgesics ,Nerve Fibers, Unmyelinated ,Camphanes ,Behavior, Animal ,business.industry ,Oxytocin receptor ,Rats ,Peripheral ,Electrophysiology ,030104 developmental biology ,Anesthesiology and Pain Medicine ,Endocrinology ,Receptors, Oxytocin ,business ,Antidiuretic Hormone Receptor Antagonists ,Locomotion ,hormones, hormone substitutes, and hormone antagonists ,030217 neurology & neurosurgery - Abstract
Background Vasopressin (AVP) seems to play a role as an antinociceptive neurohormone, but little is known about the peripheral site of action of its antinociceptive effects. Moreover, AVP can produce motor impairment that could be confused with behavioural antinociception. Finally, it is not clear which receptor is involved in the peripheral antinociceptive AVP effects. Methods In anaesthetized rats with end-tidal CO2 monitoring, extracellular unitary recordings were performed, measuring the evoked activity mediated by Aβ-, Aδ-, C-fibres and post-discharge. Behavioural nociception and motor impairment were evaluated under subcutaneous AVP (0.1–10 μg) using formalin and rotarod tests. Selective antagonists to vasopressin (V1AR) or oxytocin receptors (OTR) were used. Additionally, vasopressin and oxytocin receptors were explored immunohistochemically in skin tissues. Results Subcutaneous AVP (1 and 10 μg/paw) induced antinociception and a transitory reduction of the end-tidal CO2. The neuronal activity associated with Aδ- and C-fibre activation was diminished, but no effect was observed on Aβ-fibres. AVP also reduced paw flinches in the formalin test and a transitory locomotor impairment was also found. The AVP-induced antinociception was blocked by the selective antagonist to V1AR (SR49059) or OTR (L368,899). Immunohistochemical evidence of skin VP and OT receptors is given. Conclusions Subcutaneous AVP produces antinociception and behavioural analgesia. Both V1a and OTR participate in those effects. Our findings suggest that antinociception could be produced in a local manner using a novel vasopressin receptor located in cutaneous sensorial fibres. Additionally, subcutaneous AVP also produces important systemic effects such as respiratory and locomotor impairment. Significance Our findings support that AVP produces peripheral antinociception and behavioural analgesia in a local manner; nevertheless, systemic effects are also presented. Additionally, this is the first detailed electrophysiological analysis of AVP antinociceptive action after subcutaneous administration. The results are reasonably explained by the demonstration of V1AR and OTR in cutaneous fibres.
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- 2017
6. Successful Pain Management with Epidural Oxytocin
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Abimael González-Hernández, Julio Morales-Gómez, Héctor Zayas-González, Estefanía Córdova-Quiroz, Marco Antonio García‐Cuevas, Juan Carlos Granados‐Mortera, Alfredo Manzano-García, and Miguel Condés-Lara
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Pharmacology ,medicine.medical_specialty ,business.industry ,MEDLINE ,Pain management ,03 medical and health sciences ,Psychiatry and Mental health ,0302 clinical medicine ,Text mining ,Oxytocin ,030202 anesthesiology ,Physiology (medical) ,medicine ,Pharmacology (medical) ,Letters to the Editor ,business ,Intensive care medicine ,030217 neurology & neurosurgery ,medicine.drug - Published
- 2016
7. Axons of Individual Dorsal Horn Neurons Bifurcated to Project in Both the Anterolateral and the Postsynaptic Dorsal Column Systems
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Gerardo Rojas-Piloni, Irma A. Tello-García, Abimael González-Hernández, Guadalupe Martínez-Lorenzana, Eloísa Rubio-Beltrán, Alfredo Manzano-García, Miguel Condés-Lara, and Internal Medicine
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0301 basic medicine ,Male ,Sensory system ,Biology ,Somatosensory system ,Rats, Sprague-Dawley ,03 medical and health sciences ,0302 clinical medicine ,Postsynaptic potential ,Neural Pathways ,medicine ,Animals ,Rats, Wistar ,Posterior Horn Cell ,Medulla Oblongata ,Ventral Thalamic Nuclei ,Lumbar Vertebrae ,General Neuroscience ,Anatomy ,Spinal cord ,Axons ,Antidromic ,Neuroanatomical Tract-Tracing Techniques ,Posterior Horn Cells ,Electrophysiology ,030104 developmental biology ,medicine.anatomical_structure ,nervous system ,Medulla oblongata ,030217 neurology & neurosurgery - Abstract
Sensory information stimulates receptors of somatosensory system neurons generating a signal that codifies the characteristics of peripheral stimulation. This information reaches the spinal cord and is relayed to supra-spinal structures through two main systems: the postsynaptic dorsal column-medial lemniscal (DC-ML) and the anterolateral (AL) systems. From the classical point of view, the DC-ML has an ipsilateral ascending pathway to the Gracilis (GRA) or Cuneate (CUN) nuclei and the AL has a contralateral ascending pathway to the ventral posterolateral (VPL) thalamic nucleus. These two systems have been the subject of multiple studies that established their independence and interactions. To analyze the ascending projections of L1-L5 spinal dorsal horn neurons in the rat, two retrograde neuronal tracers were injected into the GRA and the VPL. Additionally, an electrophysiological study was performed by applying electrical stimulation at the GRA or VPL and recording antidromic evoked activity in single unit spinal cord cells. Importantly, a subset of spinal dorsal horn neurons exhibited double staining, indicating that these neurons projected to both the GRA and the VPL. These double-stained neurons were located on both sides of the dorsal horn of the spinal cord. The spinal dorsal horn neurons exhibited antidromic and collision activities in response to both GRA and VPL electrical activation. These results show spinal cord neurons with bifurcated bilateral projections to both the DC-ML and AL systems. Based on these results, we named these neurons bilateral and bifurcated cells.
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- 2017
8. Role of central oxytocin and dopamine systems in nociception and their possible interactions: suggested hypotheses
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Alfredo Manzano-García and Mohammed Gamal-Eltrabily
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0301 basic medicine ,Central Nervous System ,Nociception ,General Neuroscience ,Dopamine ,Nucleus accumbens ,Insular cortex ,Oxytocin ,Amygdala ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Hypothalamus ,medicine ,Animals ,Humans ,Psychology ,Neuroscience ,030217 neurology & neurosurgery ,Anterior cingulate cortex ,medicine.drug - Abstract
Central oxytocin and dopamine have an important role in the process of nociception at the spinal level as well as supraspinal structures, e.g. anterior cingulate cortex, insular cortex, amygdala, nucleus accumbens, and hypothalamus. Many studies have pointed out the importance of both systems in the pain descending modulatory system and in pain-related symptoms in some chronic disorders, e.g. Parkinson disease and fibromyalgia. The interaction between oxytocin and dopamine systems has been addressed in some motivational behaviors, e.g. maternal and sexual behaviors, pair bonding, and salience. In this aspect, we propose that an oxytocin-dopamine interaction could be present in nociception, and we also explain the possible hypotheses of such an interaction between these systems.
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- 2017
9. A new role of growth hormone and insulin growth factor receptor type 1 in neonatal inflammatory nociception
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Mohammed Gamal-Eltrabily and Alfredo Manzano-García
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Nociception ,medicine.medical_specialty ,Journal Club ,medicine.medical_treatment ,Inflammation ,lcsh:RD78.3-87.3 ,03 medical and health sciences ,0302 clinical medicine ,Growth factor receptor ,030202 anesthesiology ,Internal medicine ,Neonatal pain ,medicine ,Receptor ,Growth hormone ,Insulin-like growth factor 1 receptor ,business.industry ,Growth factor ,Insulin ,IGFr1 ,Anesthesiology and Pain Medicine ,Endocrinology ,lcsh:Anesthesiology ,Commentary ,Systemic administration ,medicine.symptom ,business ,030217 neurology & neurosurgery - Abstract
Neonatal inflammation produces nociception by a local decrease of growth hormone and an increment of the insulin growth factor 1-1R. Systemic growth hormone prevents the development of nociception., Growth hormone (GH) and insulin growth factor 1 (IGF1) are implicated in nociceptive processing; it has been reported that the latter participates in neonatal inflammatory nociception. In the target article, the authors propose that local inflammation evoked by carrageenan administration in mice produces a decrease in the local GH levels and an increment of IGF1 receptors type 1 expression, this produces behavioral nociception and peripheral sensitization that can be prevented by GH systemic administration pretreatment.
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- 2017
10. Peripheral oxytocin receptors inhibit the nociceptive input signal to spinal dorsal horn wide-dynamic-range neurons
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Alfredo Manzano-García, Miguel Condés-Lara, Irma A. Tello-García, Carlos Arámburo, Abimael González-Hernández, Guadalupe Martínez-Lorenzana, and Martha Carranza
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0301 basic medicine ,Male ,Nociception ,Time Factors ,Calcitonin Gene-Related Peptide ,Action Potentials ,Oxytocin ,Piperazines ,03 medical and health sciences ,0302 clinical medicine ,Formaldehyde ,Lectins ,Premovement neuronal activity ,Medicine ,Animals ,Rats, Wistar ,Receptor ,Pain Measurement ,Nerve Fibers, Unmyelinated ,Camphanes ,Dose-Response Relationship, Drug ,business.industry ,Spinal cord ,Oxytocin receptor ,Electric Stimulation ,Rats ,Posterior Horn Cells ,Electrophysiology ,030104 developmental biology ,Anesthesiology and Pain Medicine ,medicine.anatomical_structure ,Neurology ,Spinal Cord ,Receptors, Oxytocin ,Terminal nerve ,Neurology (clinical) ,Sciatic nerve ,business ,Neuroscience ,030217 neurology & neurosurgery ,Psychomotor Performance - Abstract
Oxytocin (OT) has emerged as a mediator of endogenous analgesia in behavioral and electrophysiological experiments. In fact, OT receptors (OTRs) in the spinal dorsal horn participate in a selective inhibition of the neuronal activity mediated by Aδ and C fibers but not Aβ fibers. This study shows that OTRs are expressed in the terminal nerve endings and are able to inhibit nociceptive neuronal firing. Indeed, local peripheral OT blocked the first sensorial activity of Aδ and C fibers recorded in the spinal cord neurons. Furthermore, using the formalin behavioral nociceptive test, we demonstrated that only ipsilateral OTR activation inhibits pain behavior. Our data are reinforced by the fact that the OTR protein is expressed in the sciatic nerve. Consistent with this, immunofluorescence of primary afferent fibers suggest that OTRs could be located in nociceptive-specific terminals of the skin. Taken together, our results suggest that OTRs could be found in nociceptive terminals and that on activation they are able to inhibit nociceptive input.
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- 2017
11. The potential role of serotonergic mechanisms in the spinal oxytocin-induced antinociception
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Javier Rodríguez-Jiménez, Beatriz Godínez-Chaparro, Guadalupe Martínez-Lorenzana, Alfredo Manzano-García, Miguel Condés-Lara, Abimael González-Hernández, and Gerardo Rojas-Piloni
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0301 basic medicine ,Male ,Nociception ,medicine.medical_specialty ,Serotonin ,Methiothepin ,Pain ,Stimulation ,Serotonergic ,Oxytocin ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,0302 clinical medicine ,Endocrinology ,Internal medicine ,Formaldehyde ,medicine ,Animals ,Rats, Wistar ,Pain Measurement ,Nucleus raphe magnus ,Endocrine and Autonomic Systems ,Chemistry ,General Medicine ,Oxytocin receptor ,Electric Stimulation ,Rats ,030104 developmental biology ,Allodynia ,Neurology ,Spinal Cord ,Hyperalgesia ,Receptors, Oxytocin ,Serotonin Antagonists ,medicine.symptom ,Neuroscience ,hormones, hormone substitutes, and hormone antagonists ,030217 neurology & neurosurgery ,medicine.drug - Abstract
The role of oxytocin (OXT) in pain modulation has been suggested. Indeed, hypothalamic paraventricular nuclei (PVN) electrical stimuli reduce the nociceptive neuronal activity (i.e., neuronal discharge associated with activation of Aδ- and C-fibers) of the spinal dorsal horn wide dynamic range (WDR) cells and nociceptive behavior. Furthermore, raphe magnus nuclei lesion reduces the PVN-induced antinociception, suggesting a functional interaction between the OXT and the serotoninergic system. The present study investigated in Wistar rats the potential role of spinal serotonergic mechanisms in the OXT- and PVN-induced antinociception. In long-term secondary mechanical allodynia and hyperalgesia induced by formalin or extracellular unitary recordings of the WDR cells we evaluated the role of 5-hydroxytryptamine (5-HT) effect on the OXT-induced antinociception. All drugs were given intrathecally (i.t.). OXT (1×10-5-1×10-4nmol) or 5-HT (1×10-3-1×10-1nmol) prevented the formalin-induced sensitization, an effect mimicked by PVN stimulation. Moreover, administration of OXT (1×10-5nmol) plus 5-HT (1×10-3nmol) at ineffective doses, produced antinociception. This effect was antagonized by: (i) d(CH2)5[Tyr(Me)2,Thr4,Tyr-NH29]OVT (oxytocin receptor antagonist; 2×10-2nmol); or (ii) methiothepin (a non-specific 5-HT1/2/5/6/7 receptor antagonist; 80nmol). Similar results were obtained with PVN stimulation plus 5-HT (5×10-5nmol). In WDR cell recordings, the PVN-induced antinociception was enhanced by i.t. 5-HT and partly blocked when the spinal cord was pre-treated with methiothepin (80nmol). Taken together, these results suggest that serotonergic mechanisms at the spinal cord level are partly involved in the OXT-induced antinociception.
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- 2016
12. Response to Letter to the Editor by Eisenach and Yaksh on 'Successful Pain Management with Epidural Oxytocin'
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Héctor Zayas-González, Abimael González-Hernández, Alfredo Manzano-García, and Miguel Condés-Lara
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0301 basic medicine ,Pharmacology ,medicine.medical_specialty ,Letter to the editor ,business.industry ,General surgery ,MEDLINE ,Pain management ,03 medical and health sciences ,Psychiatry and Mental health ,030104 developmental biology ,0302 clinical medicine ,Oxytocin ,Physiology (medical) ,Anesthesia ,Medicine ,Pharmacology (medical) ,Letters to the Editor ,business ,030217 neurology & neurosurgery ,medicine.drug - Published
- 2016
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