49 results on '"Angström T"'
Search Results
2. Predictive value of bronchoalveolar lavage cell analysis in sarcoidosis.
- Author
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Bjermer, L, Rosenhall, L, Angström, T, and Hällgren, R
- Abstract
Patients with histopathologically proved sarcoidosis were studied serially by means of bronchoalveolar lavage, initially at the time of diagnosis and then six and 12 months later. Two years later they were evaluated by chest radiography and lung function tests and classified in terms of recovery or progression over the previous two years. The recovery of lymphocytes and granulocytes in lavage fluid was of limited prognostic value for persistent lung disease. In contrast, patients with increased numbers of mast cells recovered by lavage were more likely to deteriorate. Significantly increased mast cell counts (greater than or equal to 0.5% of total cells recovered) were seen in at least one lavage investigation in 15 of the 16 patients with more active and progressive disease, but in only eight of 23 patients with inactive disease (p less than 0.001). A persistent increase of mast cells on serial measurement occurred in nine of the 16 patients with active disease and in four of the 23 patients in whom the disease was inactive (p less than 0.02). The finding in the two subsequent lavages of lymphocytosis (lymphocytes greater than 30% of recovered cells) or neutrophilia (neutrophils greater than 15%) combined with mastocytosis (mast cells greater than or equal to 0.5%) occurred in nine of the 16 patients with active disease but in no patients with inactive disease. [ABSTRACT FROM AUTHOR]
- Published
- 1988
3. Prognostic significance of transrectal fine-needle aspiration biopsy findings after orchiectomy for carcinoma of the prostate
- Author
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R. Tomic, Bergman B, Hietala So, and Angström T
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Male ,Pathology ,medicine.medical_specialty ,Urology ,Prostate ,Cytology ,Biopsy ,medicine ,Carcinoma ,Humans ,Orchiectomy ,Aged ,Neoplasm Staging ,medicine.diagnostic_test ,business.industry ,Biopsy, Needle ,Prostatic Neoplasms ,Middle Aged ,medicine.disease ,Prognosis ,medicine.anatomical_structure ,Fine-needle aspiration ,Cancer cell ,business ,Biopsy findings ,Follow-Up Studies - Abstract
In 48 patients with prostatic carcinoma two types of cell patterns were found at fine-needle aspiration biopsy at follow-up 6 and 12 months after orchiectomy: (1) unmodified carcinoma cells or (2) carcinoma cells with regressive changes. Objective clinical regression at 36 months was significantly more frequent in patients with regressively transformed cancer cells at 6 and/or 12 months than in patients with unmodified carcinoma cells: 73 and 32%, respectively. Ten percent of the patients with regressively transformed carcinoma cells and 41% of the patients with unmodified cancer cells died of prostatic carcinoma. The cytologic findings at 6 and/or 12 months after orchiectomy is thus of importance for predicting the prognosis.
- Published
- 1985
4. Prognostic Significance of Transrectal Fine-Needle Aspiration Biopsy Findings after Orchiectomy for Carcinoma of the Prostate
- Author
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Tomic, R., primary, Bergman, B., additional, Hietala, S.-O., additional, and Angström, T., additional
- Published
- 1985
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5. p16INK4a and laminin-5gamma2 chain expression during the progression of cervical neoplasia.
- Author
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Wang JL, Andersson S, Li X, Hellström AC, Auer G, Angström T, Lindström MS, and Wallin KL
- Subjects
- Adult, Basement Membrane metabolism, Biomarkers, Tumor genetics, Cell Adhesion Molecules genetics, Cyclin-Dependent Kinase Inhibitor p16 genetics, Disease Progression, Female, Humans, Immunohistochemistry, Middle Aged, Proliferating Cell Nuclear Antigen metabolism, Sweden, Time Factors, Kalinin, Biomarkers, Tumor metabolism, Cell Adhesion Molecules metabolism, Cyclin-Dependent Kinase Inhibitor p16 metabolism, Gene Expression Regulation, Neoplastic, Uterine Cervical Neoplasms metabolism
- Abstract
p16INK4a, laminin-5gamma2 chain, and PCNA were investigated to compare the expression levels in relation to histological diagnosis and time for progression. The material consisted of 37 normal cervical tissues, 35 with different grades of CIN, and 11 invasive cervical cancers. Our results showed a reduction of basement membrane staining for laminin-5gamma2 chain from 78.4% in normal squamous epithelium to 27.8% in CIN3 (p < 0.001). The intracytoplasmic staining for laminin-5gamma2 chain increases with severity of lesion. The same trend was observed with p16INK4a and PCNA expression (p < 0.001). Co-expression of p16INK4a and PCNA was seen in 85.7% of samples. Cases that were laminin-5gamma2 chain BM - /p16INK4a+/PCNA+ have the shortest interval time (average: 46.8+/-36.3 months) for progression, while cases with laminin-5gamma2 chain BM + /p16INK4a-/PCNAPCNA--have the longest time interval (average: 110.2+/-52.7 months) (p < 0.05). Thus co-expression of p16INK4a, laminin-5gamma2 chain and PCNA may be valuable for the prediction progression of cervical neoplasia.
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- 2006
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6. The clinicopathologic significance of laminin-5 gamma2 chain expression in cervical squamous carcinoma and adenocarcinoma.
- Author
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Andersson S, Hellström AC, Angström T, Stendahl U, Auer G, and Wallin KL
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- Adenocarcinoma mortality, Adenocarcinoma pathology, Age Factors, Carcinoma, Squamous Cell mortality, Carcinoma, Squamous Cell pathology, Female, Humans, Neoplasm Staging, Prognosis, Survival Analysis, Uterine Cervical Neoplasms mortality, Uterine Cervical Neoplasms pathology, Adenocarcinoma metabolism, Carcinoma, Squamous Cell metabolism, Laminin biosynthesis, Uterine Cervical Neoplasms metabolism
- Abstract
Carcinoma of the uterine cervix is one of the most prevalent malignancies among women in developing countries and the third most common type worldwide. Squamous cell carcinoma predominates in the cervix uteri, while adenocarcinoma and adenosquamous carcinomas represent about 10-15% of all cervical cancers. Many studies have confirmed that the human papillomavirus (HPV) is the most important etiologic factor in the development of cervical cancer. The aim of our study was to investigate the expression of the laminin-5 gamma2 chain in primary malignancies of the cervix uteri and to focus on the clinicopathologic significance of the expression of the laminin-5 gamma2 chain in cervical squamous carcinoma and adenocarcinoma with respect to age and survival of the patients. The study consisted of a total of 89 cases of invasive cervical cancer (54 squamous carcinomas and 35 adenocarcinomas). The laminin-5 gamma2 chain was found in 80% of all the squamous carcinoma and in 66% of cervical adenocarcinoma. There was no correlation of the high expression of laminin-5 with survival. The univariate analysis in squamous cell carcinoma showed that factors such as the stage of the disease and positive lymph nodes had an impact on the survival of the patients, whereas in the multivariate analysis, only age at diagnosis was an independent prognostic factor. However, in cases with cervical adenocarcinoma, only the stage of the disease was an independent prognostic factor. There was no difference between HPV-positive and HPV-negative tumors concerning the high expression of laminin-5 gamma2 chain. Our results indicate that the majority of the primary cervical tumors, especially squamous cell carcinoma, showed expression of laminin-5 gamma2 chain immunoreactivity. Independent prognostic values for the survival of the patients were age and stage of the disease.
- Published
- 2005
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7. p16INK4A and p14ARF expression pattern by immunohistochemistry in human papillomavirus-related cervical neoplasia.
- Author
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Wang JL, Zheng BY, Li XD, Nokelainen K, Angström T, Lindström MS, and Wallin KL
- Subjects
- Adult, Aged, Carcinoma, Squamous Cell etiology, Carcinoma, Squamous Cell metabolism, Carcinoma, Squamous Cell pathology, Disease Progression, Female, Humans, Immunohistochemistry, Middle Aged, Neoplasm Invasiveness, Papillomavirus Infections virology, Proliferating Cell Nuclear Antigen analysis, Uterine Cervical Neoplasms etiology, Uterine Cervical Neoplasms metabolism, Uterine Cervical Dysplasia etiology, Uterine Cervical Dysplasia metabolism, Uterine Cervical Dysplasia pathology, Cyclin-Dependent Kinase Inhibitor p16 biosynthesis, Papillomaviridae, Papillomavirus Infections complications, Tumor Suppressor Protein p14ARF biosynthesis, Uterine Cervical Neoplasms pathology
- Abstract
Human papillomavirus is known to play an important etiological role in the genesis of cervical cancer, but only a very small proportion of infected women develop invasive cervical cancer. The purpose of cervical cancer prevention is early diagnosis of its precursors. The molecular detection of human papillomavirus DNA as a diagnostic test to cervical carcinogenesis gave a low positive predictive value as compared to the use of biomarkers. p16INK4A and possibly p14ARF have been proposed as putative surrogate biomarkers that would allow identification of dysplastic cervical epithelia. Serial consecutive biopsies representing normal cervical epithelium to cervical intraepithelial neoplasia and/or invasive cervical cancer were stained with immunohistochemistry for p16INK4A, p14ARF and proliferating cell nuclear antigen. The positive rates of these markers were significantly higher in cervical intraepithelial neoplasia and in squamous cell carcinoma than in normal cervix (P<0.01). No significant difference was noted between lesions progressing from cervical intraepithelial neoplasia to squamous cell carcinoma for both p16INK4A and p14ARF expression (P>0.05). For both biomarkers, nuclear staining was predominantly seen. However, the cytoplasmic stain of p16INK4A increased with disease progression and the pattern of expression varied between different tumors and its location within the lesion. Both nuclear and cytoplasmic staining with p16INK4A and p14ARF of affected epithelial cells were considered positive. In the adjacent normal tissue to cervical neoplasia, the positive rates of p16INK4A, p14ARF and proliferating cell nuclear antigen expression were higher than those found distant to these lesions but the findings did not reach statistical significance. No correlation was seen between the human papillomavirus types detected and the expression of p16INK4a and p14ARF. In conclusion, overexpression of p16INK4A and p14ARF act as potential biomarkers for cervical cancer progression from premalignant lesions.
- Published
- 2005
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8. Telomere attrition predominantly occurs in precursor lesions during in vivo carcinogenic process of the uterine cervix.
- Author
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Zhang A, Wang J, Zheng B, Fang X, Angström T, Liu C, Li X, Erlandsson F, Björkholm M, Nordenskjörd M, Gruber A, Wallin KL, and Xu D
- Subjects
- DNA Damage, DNA-Binding Proteins, Female, Humans, Neoplasm Invasiveness, Telomerase genetics, Uterine Cervical Neoplasms pathology, Uterine Cervical Dysplasia genetics, Precancerous Conditions genetics, Telomere genetics, Uterine Cervical Neoplasms genetics
- Abstract
Although human papillomavirus (HPV) has been defined as the pathogen for cervical carcinomas, molecular events underlying the oncogenic process are unclear. As telomere dysfunction-mediated chromosomal instability and telomerase activation have been suggested as key events in carcinogenesis, we dissected the dynamic changes in telomere length, checkpoint response, and temporal profile of telomerase expression during the evolution from precursor lesions (cervical intraepithelial neoplasia, CINs) to invasive cancers of the uterine cervix in sequential samples from 16 patients. Telomeres were significantly shortened in all CIN samples and no further substantial attritions occurred in most cases with the acquisition of malignant phenotype. Very short telomeres were coupled with constitutive activation of the DNA damage response pathway (Chk2 phosphorylation) and increased cellular proliferation in those cervical specimens. Telomerase reverse transcriptase (hTERT) expression was preferably induced at advanced CINs or invasive cancers. The present finding demonstrates that excessive telomere shortening predominantly occurs in the early carcinogenesis of the uterine cervix largely prior to telomerase activation. Widespread over-erosion of telomeres or telomere dysfunction in very early stages of cervical tumorigenesis might fuel transformation processes by driving chromosomal instability.
- Published
- 2004
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9. Gelatinases A and B (MMP-2 and MMP-9) in endometrial cancer-MMP-9 correlates to the grade and the stage.
- Author
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Aglund K, Rauvala M, Puistola U, Angström T, Turpeenniemi-Hujanen T, Zackrisson B, and Stendahl U
- Subjects
- Female, Humans, Immunohistochemistry, Neoplasm Staging, Paraffin Embedding, Predictive Value of Tests, Prognosis, Survival Rate, Endometrial Neoplasms enzymology, Endometrial Neoplasms pathology, Matrix Metalloproteinase 2 metabolism, Matrix Metalloproteinase 9 metabolism
- Abstract
Objective: Matrix metalloproteinases (MMPs) have been linked to aggressive behavior in several malignancies. Gelatinases (MMP-2 and MMP-9) in particular are prognostic factors in many adeno- and epithelial cancers. However, no conclusive data exist concerning the role of gelatinases in endometrial cancer., Methods: Eighty-eight patients with endometrial cancer, treated between 1988 and 1993 in Umeå University Hospital, were included. MMP-2 and MMP-9 proteins were analyzed immunohistochemically from paraffin-embedded tissues by using specific monoclonal antibodies. The staining results were compared to the clinical data., Results: Fifty-two percent of the cases were positive for MMP-9 and 72% for MMP-2. The overexpression of the proteins of either MMP-2 or MMP-9 was associated with poor survival. The predictive value of MMP-2 expression was most distinct in stage I cancers. An association was found between the positivity of MMP-2 and MMP-9. Only 3% of the cases were highly positive for both gelatinases and 18% of the cases were negative for both MMPs. Both gelatinases correlated to the histological grade. MMP-9 also correlated to the clinical stage of the disease, whereas MMP-2 did not. There was no apparent association with either depth of invasion, menopausal status, or the age of the patient., Conclusion: MMP-2 and MMP-9 could serve as markers for the clinical behavior of endometrial cancer. They may further be linked to a tendency to cancer relapse. Thus, these gelatinases may turn out to be potentially useful in decision making about the need for an adjuvant treatment.
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- 2004
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10. Predictive significance of the alterations of p16INK4A, p14ARF, p53, and proliferating cell nuclear antigen expression in the progression of cervical cancer.
- Author
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Wang JL, Zheng BY, Li XD, Angström T, Lindström MS, and Wallin KL
- Subjects
- Adult, Aged, Cervix Uteri metabolism, DNA chemistry, DNA, Viral metabolism, Disease Progression, Epithelium metabolism, Female, Humans, Immunohistochemistry, Inflammation, Middle Aged, Neoplasms, Squamous Cell metabolism, Papillomaviridae genetics, Polymerase Chain Reaction, Risk Factors, Time Factors, Uterine Cervical Neoplasms diagnosis, Uterine Cervical Neoplasms pathology, Uterine Cervical Dysplasia metabolism, Cyclin-Dependent Kinase Inhibitor p16 biosynthesis, Proliferating Cell Nuclear Antigen biosynthesis, Tumor Suppressor Protein p14ARF biosynthesis, Tumor Suppressor Protein p53 biosynthesis, Uterine Cervical Neoplasms metabolism
- Abstract
Purpose: The purpose of this research was to evaluate the clinical significance of p16INK4A, p14ARF, p53, and proliferating cell nuclear antigen (PCNA) expression in tumor progression of cervical cancer., Design: Seventeen patients (40 samples) with consecutive cervical lesions from normal squamous epithelium, inflammation of the cervix to cervical intraepithelial neoplasm (CIN) and invasive cervical squamous cell cancer (SCC), or from CIN to SCC were collected for this study. Expression of p16INK4A, p14ARF, p53, and PCNA were detected by immunohistochemistry on paraffin-embedded sections. Human papillomavirus DNA was detected simultaneously with PCR and typed according to its DNA sequence., Results: p16INK4A overexpression was significantly higher in CIN (75%) and in SCC (75%) than in normal or inflammation of the cervix (12.5%; P < 0.01, P < 0.05, respectively). The positive rate of p14ARF expression was higher in SCC (83%) than in normal/inflammation of the cervix (25%; P < 0.05). PCNA expression was negative in normal or inflammation of the cervix, but an increased in expression was seen in 63.2% in CIN and 100% in SCC (P < 0.01, P < 0.05). When the time interval for disease progression from initial biopsy to CIN 3 or invasive cancer was compared with states of p16INK4A expression, cases stained positive for p16INK4A progressed within 64.2 months as compared with 122.3 months among those stained negatively (P < 0.01). Cases with increased p14ARF expression also had a short time interval for disease progression of 78.8 months as compared with 108.3 months in cases that were p14ARF negative. Cases with stable or decreased p53 expression had the shortest time interval for progression of 32.3 months in contrast to cases with no p53 expression (113.9 months). However, cases with increasing p53 expression progressed within 60.8 months., Conclusions: Our results suggested that altered states of p16INK4A, p14ARF, p53, and PCNA may be valuable markers to predict the progression of cervical neoplasia.
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- 2004
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11. Genetic alterations in cervical carcinomas: frequent low-level amplifications of oncogenes are associated with human papillomavirus infection.
- Author
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Zhang A, Månér S, Betz R, Angström T, Stendahl U, Bergman F, Zetterberg A, and Wallin KL
- Subjects
- Base Sequence, Chromosome Mapping, DNA Primers, DNA, Viral analysis, Female, Humans, Immunohistochemistry, In Situ Hybridization, Fluorescence, Neoplasm Staging, Papillomavirus Infections pathology, Polymerase Chain Reaction methods, Tumor Virus Infections pathology, Uterine Cervical Neoplasms pathology, Gene Amplification, Oncogenes, Papillomaviridae, Papillomavirus Infections genetics, Tumor Virus Infections genetics, Uterine Cervical Neoplasms genetics, Uterine Cervical Neoplasms virology
- Abstract
The development of cervical carcinoma is closely associated with HPV infection. However, other genetic alterations also play an important role. In this study, we analyzed copy number alterations of several oncogene loci in a panel of 84 cervical tumors. Sixty-five (77%) tumors were HPV DNA-positive, and most were infected with type 16 or type 18 or both. The oncogenes studied include PIK3CA at 3q26.3, TERT at 5p15.33, C-MYC at 8q24, CCND1 at 11q13.3, ERBB2 at 17q21.2 and locus region 20q13.2. Amplification of 1 or more genes was detected in 55 (65%) cases using interphase FISH. PIK3CA was amplified in 43% of tumors, followed by TERT (33%), 20q13.2 (30%), ERBB2 (29%), C-MYC (25%) and CCND1 (12%). Most tumors showed low-level amplification with 3-7 copies of these genes, and complex changes involving 3 or more genes occur more frequently in tumors at advanced stages. Increased protein expression of c-erbB2 and c-myc was observed in tumors with the corresponding gene amplification. Oncogene alterations were found more often in HPV-infected cases, particularly for C-MYC and TERT. These findings indicate that HPV-associated cervical carcinomas bear frequent alterations of these genes, which may have critical biologic impact on the development and progression of carcinoma of the uterine cervix., (Copyright 2002 Wiley-Liss, Inc.)
- Published
- 2002
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12. A population-based prospective study of Chlamydia trachomatis infection and cervical carcinoma.
- Author
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Wallin KL, Wiklund F, Luostarinen T, Angström T, Anttila T, Bergman F, Hallmans G, Ikäheimo I, Koskela P, Lehtinen M, Stendahl U, Paavonen J, and Dillner J
- Subjects
- Adult, Aged, Biopsy, Chlamydia Infections epidemiology, DNA, Bacterial analysis, DNA, Viral analysis, Female, Humans, Middle Aged, Papanicolaou Test, Papillomaviridae genetics, Polymerase Chain Reaction, Prospective Studies, Risk, Sweden epidemiology, Uterine Cervical Neoplasms diagnosis, Uterine Cervical Neoplasms virology, Vaginal Smears, Chlamydia Infections diagnosis, Chlamydia trachomatis genetics, Uterine Cervical Neoplasms microbiology
- Abstract
Persistent human papillomavirus (HPV) infection is an established cause of cervical cancer, but the role of other sexually transmitted agents, most notably Chlamydia trachomatis, has not been well defined. The women participating in the population-based cervical cancer screening program in Västerbotten county of Northern Sweden were followed up for up to 26 years to identify 118 women who developed cervical cancer after having had a normal Pap smear (on average 5.6 years later; range 0.5 months-26 years). As controls, we selected another 118 women who were matched by birth cohort, time-point of sampling of the baseline normal smear and who had a normal smear at the time when the corresponding case was diagnosed with cancer. The Pap smears and cervical cancer biopsies were analyzed by PCR for C. trachomatis DNA and for HPV DNA. At baseline, C. trachomatis DNA was present in 8% of cases but not among any one of the controls. The relative risk for cervical cancer associated with past C. trachomatis infection, adjusted for concomitant HPV DNA positivity, was 17.1 (95% CI 2.6-infinity). The presence of C. trachomatis and of HPV were not interrelated. Whereas C. trachomatis was primarily found in specimens taken many years before cancer diagnosis, HPV DNA was associated with a short lag time before cancer diagnosis. Whereas most women who were HPV DNA-positive in the prediagnostic smear were also positive for the same virus in the cervical cancer biopsy, none of the women were positive for C. trachomatis in both the prediagnostic smear and in the subsequent cervical cancer. In conclusion, a prior cervical C. trachomatis infection was associated with an increased risk for development of invasive cervical cancer., (Copyright 2002 Wiley-Liss, Inc.)
- Published
- 2002
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13. Amplification of the telomerase reverse transcriptase (hTERT) gene in cervical carcinomas.
- Author
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Zhang A, Zheng C, Hou M, Lindvall C, Wallin KL, Angström T, Yang X, Hellström AC, Blennow E, Björkholm M, Zetterberg A, Gruber A, and Xu D
- Subjects
- Carcinoma complications, Carcinoma metabolism, Carcinoma virology, DNA-Binding Proteins, Female, Gene Dosage, Humans, Papillomaviridae isolation & purification, Papillomavirus Infections complications, Papillomavirus Infections genetics, Paraffin Embedding, Telomerase biosynthesis, Tumor Cells, Cultured, Tumor Virus Infections complications, Tumor Virus Infections genetics, Uterine Cervical Neoplasms metabolism, Uterine Cervical Neoplasms virology, Uterine Cervical Dysplasia complications, Uterine Cervical Dysplasia genetics, Uterine Cervical Dysplasia metabolism, Uterine Cervical Dysplasia virology, Carcinoma genetics, Gene Amplification genetics, Telomerase genetics, Uterine Cervical Neoplasms genetics
- Abstract
The expression of telomerase reverse transcriptase (hTERT), the catalytic component of the telomerase complex, is required for activation of telomerase during immortalization and transformation of human cells. However, the biochemical and genetic mechanisms governing hTERT expression remain to be elucidated. In the present study, we examined hTERT amplification as a potential genetic event contributing to telomerase activation in cervical carcinomas. An amplification of the hTERT gene was found in 1/4 cervical cancer cell lines and 21/88 primary tumor samples derived from the patients with cervical carcinomas. An increase in the hTERT copy number was significantly correlated with higher levels of hTERT protein expression. Moreover, the hTERT alterations with the enhanced hTERT expression were exclusively observed in those tumors with high-risk human papillomavirus infection. Taken together, the hTERT gene amplification, directly or indirectly targeted by human papillomavirus, may be one of the driving forces responsible for upregulation of hTERT expression and activation of telomerase in cervical cancers., (Copyright 2002 Wiley-Liss, Inc.)
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- 2002
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14. Regular disappearance of the human papillomavirus genome after conization of cervical dysplasia by carbon dioxide laser.
- Author
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Kjellberg L, Wadell G, Bergman F, Isaksson M, Angström T, and Dillner J
- Subjects
- Adult, DNA, Viral analysis, Female, Humans, Middle Aged, Postoperative Period, Uterine Cervical Dysplasia pathology, Conization, Genome, Viral, Laser Therapy, Papillomaviridae genetics, Uterine Cervical Dysplasia surgery, Uterine Cervical Dysplasia virology
- Abstract
Objective: We wished to evaluate the effectiveness of treatment of cervical dysplasia by laser conization in relation to persistence of human papillomavirus after treatment., Study Design: Of 203 women referred to colposcopy because of an abnormal Papanicolaou smear, 149 women could be followed up for 3 years. A total of 108 women were treated by carbon dioxide laser excision, 4 women were treated by carbon dioxide laser evaporation, and 37 women were merely followed up. Cervical samples were taken before treatment and at follow-up 3 years later and were analyzed by nested general primer polymerase chain reaction for human papillomavirus deoxyribonucleic acid., Results: Among women treated by laser conization, 82 (73.2%) had positive results for human papillomavirus deoxyribonucleic acid before treatment. Three women (2.7%) had a positive finding at follow-up, but no woman had the same human papillomavirus type on both occasions. Eighty-eight women had grade 1 to grade 3 cervical intraepithelial neoplasia before treatment, whereas during follow-up only 2 squamous cells atypias were found., Conclusion: The human papillomavirus genome present before treatment was regularly cleared, and there was also no recurrence of dysplasia. The results suggest that human papillomavirus testing is useful for monitoring the efficacy of treatment and that treatment modalities resulting in clearance of human papillomavirus should be favored.
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- 2000
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15. [ASCUS, CIN I and vaginal cytological diagnosis--a comment].
- Author
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Angström T and Ryd W
- Subjects
- Carcinoma, Squamous Cell classification, Female, Humans, Terminology as Topic, Uterine Cervical Dysplasia classification, Uterine Cervical Dysplasia pathology, Uterine Cervical Neoplasms classification, Carcinoma, Squamous Cell pathology, Uterine Cervical Neoplasms pathology, Vaginal Smears methods
- Published
- 2000
16. Smoking, diet, pregnancy and oral contraceptive use as risk factors for cervical intra-epithelial neoplasia in relation to human papillomavirus infection.
- Author
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Kjellberg L, Hallmans G, Ahren AM, Johansson R, Bergman F, Wadell G, Angström T, and Dillner J
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- Adult, Case-Control Studies, Confounding Factors, Epidemiologic, Female, Humans, Middle Aged, Papillomaviridae, Pregnancy, Risk Factors, Uterine Cervical Neoplasms virology, Uterine Cervical Dysplasia virology, Contraceptives, Oral adverse effects, Diet, Papillomavirus Infections complications, Parity, Smoking adverse effects, Tumor Virus Infections complications, Uterine Cervical Neoplasms etiology, Uterine Cervical Dysplasia etiology
- Abstract
Smoking, nutrition, parity and oral contraceptive use have been reported as major environmental risk factors for cervical cancer. After the discovery of the very strong link between human papillomavirus (HPV) infection and cervical cancer, it is unclear whether the association of these environmental factors with cervical cancer reflect secondary associations attributable to confounding by HPV, if they are independent risk factors or whether they may act as cofactors to HPV infection in cervical carcinogenesis. To investigate this issue, we performed a population-based case-control study in the Vasterbotten county of Northern Sweden of 137 women with high-grade cervical intra-epithelial neoplasia (CIN 2-3) and 253 healthy age-matched women. The women answered a 94-item questionnaire on diet, smoking, oral contraceptive use and sexual history and donated specimens for diagnosis of present HPV infection (nested polymerase chain reaction on cervical brush samples) and for past or present HPV infections (HPV seropositivity). The previously described protective effects of dietary micronutrients were not detected. Pregnancy appeared to be a risk factor in the multivariate analysis (P < 0.0001). Prolonged oral contraceptive use and sexual history were associated with CIN 2-3 in univariate analysis, but these associations lost significance after taking HPV into account. Smoking was associated with CIN 2-3 (odds ratio (OR) 2.6, 95% confidence interval (CI) 1.7-4.0), the effect was dose-dependent (P = 0.002) and the smoking-associated risk was not affected by adjusting for HPV, neither when adjusting for HPV DNA (OR 2.5, CI 1.3-4.9) nor when adjusting for HPV seropositivity (OR 3.0, CI 1.9-4.7). In conclusion, after taking HPV into account, smoking appeared to be the most significant environmental risk factor for cervical neoplasia.
- Published
- 2000
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17. Type-specific persistence of human papillomavirus DNA before the development of invasive cervical cancer.
- Author
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Wallin KL, Wiklund F, Angström T, Bergman F, Stendahl U, Wadell G, Hallmans G, and Dillner J
- Subjects
- Adenocarcinoma etiology, Adenocarcinoma pathology, Adenocarcinoma virology, Adult, Carcinoma, Squamous Cell etiology, Carcinoma, Squamous Cell pathology, Carcinoma, Squamous Cell virology, Case-Control Studies, Chronic Disease, Female, Humans, Middle Aged, Neoplasm Invasiveness, Papanicolaou Test, Papillomaviridae classification, Papillomaviridae genetics, Population Surveillance, Prospective Studies, Risk Factors, Sensitivity and Specificity, Uterine Cervical Neoplasms pathology, Uterine Cervical Neoplasms virology, Vaginal Smears, Cervix Uteri virology, DNA, Viral analysis, Papillomaviridae isolation & purification, Papillomavirus Infections complications, Tumor Virus Infections complications, Uterine Cervical Neoplasms etiology
- Abstract
Background: Infection with the human papillomavirus (HPV) has been established as a cause of cervical cancer, but the association between a positive test for HPV DNA and the risk of the subsequent development of invasive cervical cancer is unknown., Methods: In a study of women who participated in a population-based screening program for cancer of the cervix in Sweden from 1969 to 1995, we compared the proportion of normal cervical smears (Pap smears) that were positive for HPV DNA among 118 women in whom invasive cervical cancer developed an average of 5.6 years later (range, 0.5 month to 26.2 years) with the proportion of HPV DNA-positive smears from 118 women who remained healthy during a similar length of follow-up (controls). The control women were matched for age to the women with cancer, and they had had two normal Pap smears obtained at time points that were similar to the times of the baseline smear and the diagnosis of cancer confirmed by biopsy in the women with cancer., Results: At baseline, 35 of the women with cancer (30 percent) and 3 of the control women (3 percent) were positive for HPV DNA (odds ratio, 16.4; 95 percent confidence interval, 4.4 to 75.1). At the time of diagnosis, 80 of the 104 women with cancer for whom tissue samples were available (77 percent) and 4 of the 104 matched control women (4 percent) were positive for HPV DNA. The HPV DNA type was the same in the base-line smear and the biopsy specimen in all of the women with cancer in whom HPV DNA was detected at base line. None of the control women had the same type of HPV in both smears., Conclusions: A single positive finding of HPV DNA in a Pap smear confers an increased risk of future invasive cervical cancer that is positive for the same type of virus as identified earlier.
- Published
- 1999
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18. A population-based study of human papillomavirus deoxyribonucleic acid testing for predicting cervical intraepithelial neoplasia.
- Author
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Kjellberg L, Wiklund F, Sjöberg I, Wadell G, Angström T, Dillner J, and Mählck CG
- Subjects
- Adult, Colposcopy, Female, Humans, Mass Screening, Middle Aged, Papillomaviridae genetics, Polymerase Chain Reaction, Uterine Cervical Neoplasms pathology, Uterine Cervical Neoplasms virology, Uterine Cervical Dysplasia pathology, Uterine Cervical Dysplasia virology, DNA, Viral analysis, Papillomaviridae isolation & purification, Papillomavirus Infections diagnosis, Tumor Virus Infections diagnosis, Uterine Cervical Neoplasms diagnosis, Uterine Cervical Dysplasia diagnosis
- Abstract
Objective: Our purpose was to determine the predictive values of primary or secondary screening for cervical human papillomavirus infection for cytologic detection of cervical intraepithelial neoplasia., Study Design: Most of the 254 women referred for colposcopy in Västerbotten County in Sweden during October 1993 through December 1995 and 320 age-matched women from the general population were screened for human papillomavirus deoxyribonucleic acid by nested general-primer polymerase chain reaction., Results: Ninety-six percent of women with high-grade cervical intraepithelial neoplasia had human papillomavirus, compared with 4% of women with normal findings (odds ratio 606; 95% confidence interval 137 to 5607). Thirty-seven percent of referred women and 48% of referred women >39 years old had mostly minor cytologic abnormalities with no human papillomavirus. The human papillomavirus-associated positive predictive value for cervical intraepithelial neoplasia was 76% in the colposcopy group and 11% in the general population, whereas the negative predictive value was >97% in both populations., Conclusion: Testing for human papillomavirus deoxyribonucleic acid seems diagnostically useful among women referred for colposcopy.
- Published
- 1998
- Full Text
- View/download PDF
19. Cellular changes in prostatic carcinoma after treatment with orchidectomy, estramustine phosphate and medroxyprogesterone acetate.
- Author
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Tomic R, Angström T, and Ljungberg B
- Subjects
- Aged, Aged, 80 and over, Biopsy, Needle, Cell Survival drug effects, Combined Modality Therapy, Dose-Response Relationship, Drug, Drug Administration Schedule, Humans, Male, Middle Aged, Prostate drug effects, Prostate pathology, Prostatic Neoplasms therapy, Antineoplastic Agents, Hormonal therapeutic use, Estramustine therapeutic use, Medroxyprogesterone Acetate therapeutic use, Orchiectomy, Prostatic Neoplasms pathology
- Abstract
Thirty-three patients with prostatic carcinoma were treated with either estramustine phosphate, orchidectomy or high dose medroxyprogesterone acetate. Therapy response was evaluated by cytological examination of fine needle aspiration biopsies performed before and after 6 weeks treatment. At follow-up, 11 of 14 patients treated with estramustine phosphate had regressive and/or degenerative changes, in 2 patients there were no prostatic carcinoma cells in the smears and in one there was a marked reduction of the number of tumour cells. In 7 of 10 patients treated with orchidectomy there was a marked reduction of the percentage of malignant cells while smears from 3 patients were unchanged. In the 8 patients treated with high dose medroxyprogesterone acetate the cell patterns were unmodified compared with before treatment. We conclude that, in contrast to the lack of effect of treatment with medroxyprogesterone acetate, treatment with orchidectomy and especially estramustine phosphate caused morphologic cellular changes in prostatic carcinoma.
- Published
- 1997
- Full Text
- View/download PDF
20. Different HLA-DR-DQ haplotypes are associated with cervical intraepithelial neoplasia among human papillomavirus type-16 seropositive and seronegative Swedish women.
- Author
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Sanjeevi CB, Hjelmström P, Hallmans G, Wiklund F, Lenner P, Angström T, Dillner J, and Lernmark A
- Subjects
- Adult, Alleles, Female, Haplotypes, Humans, Middle Aged, Uterine Cervical Neoplasms immunology, Uterine Cervical Neoplasms virology, Uterine Cervical Dysplasia immunology, Uterine Cervical Dysplasia virology, Antibodies, Viral blood, HLA-DQ Antigens genetics, HLA-DR Antigens genetics, Papillomaviridae immunology, Uterine Cervical Neoplasms etiology, Uterine Cervical Dysplasia etiology
- Abstract
To analyze whether HLA may be a determinant of the risk of developing cervical cancer precursor lesions, the association between HLA and cervical neoplasia among HPV16-seropositive and -negative subjects was determined in a population-based cohort in the Västerbotten county of Northern Sweden. HLA genotyping of DR and DQ was done by PCR in 74 patients and 164 healthy controls matched for age, sex and area of residence. The presence of DQA1*0102 was weakly associated with cervical neoplasia in HPV16-seropositive patients. DQB1*0602 was weakly associated with disease in all patients, but was strongly increased among HPV16-seropositive patients compared to HPV16-seropositive controls. DR15 had an association with disease that was particularly strong among HPV16-seropositive subjects. The haplotype DQA1*0102-DQB1*0602 (DQ6) was also weakly associated with disease in all patients and significantly increased among HPV16-positive patients when compared to HPV16-positive controls. A similar association was seen when analysis was restricted to CIN 2-3 patients. DQA1*0501-DQB1*0301 (DQ7) was more common among HPV16-negative patients than among HPV16-negative controls and was also more common among HPV16-negative patients than among HPV16-positive patients. In conclusion, DQA1*0102-DQB1*0602 (DQ6) is associated with an increased risk of cervical neoplasia among HPV16-seropositive subjects and DQA1*0501-DQB1*0301 (DQ7) with an increased risk among HPV16-seronegative subjects.
- Published
- 1996
- Full Text
- View/download PDF
21. A prospective study on the risk of cervical intra-epithelial neoplasia among healthy subjects with serum antibodies to HPV compared with HPV DNA in cervical smears.
- Author
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Chua KL, Wiklund F, Lenner P, Angström T, Hallmans G, Bergman F, Sapp M, Schiller J, Wadell G, Hjerpe A, and Dillner J
- Subjects
- Adult, Age Factors, Cohort Studies, Female, Humans, Middle Aged, Papanicolaou Test, Papillomavirus Infections virology, Polymerase Chain Reaction, Prospective Studies, Risk Factors, Sweden, Tumor Virus Infections virology, Vaginal Smears, Antibodies, Viral blood, Cervix Uteri virology, DNA, Viral analysis, Papillomaviridae genetics, Papillomaviridae immunology, Uterine Cervical Dysplasia virology
- Abstract
To estimate the risk of developing cervical intra-epithelial neoplasia (CIN) among women exposed to human papillomavirus (HPV) type 16, we performed a prospective study in a population-based cohort of more than 15,000 women followed for 34.9 months. Seventy-four women developed CIN during follow-up and were matched for age, time of sampling and area of residence with 148 women who remained CIN-free during follow-up. The blood samples taken at enrollment were tested for serum antibodies to HPV types 16, 18 and 33 capsids. Cervical smears or biopsies were analyzed for the presence of HPV DNA by nested PCR using HPV general primers and by HPV 16- and 18-type-specific PCR HPV serology and HPV-PCR were in good agreement, particularly when the blood sample and the Pap smear were taken less than 6 months apart. HPV DNA was found in 88% of cases and 4% of controls, whereas HPV 16 DNA was present in 44% of cases and in 1 of 142 controls. HPV-16-seropositive women had a 3-fold increased risk of developing CIN. The risk was highest among women younger than 35 years of age, of whom an estimated 3.4% of HPV-16-seropositive and 0.5% of seronegative women developed CIN. Since the risk associated with HPV-16 seropositivity (a measure of past or present infection) was 35-fold lower than that of HPV DNA (present infection), most infections appear to be eliminated before CIN develops. In conclusion, HPV 16 infection does confer an excess risk of CIN development, and HPV DNA detection has a high predictive value for the presence of high-grade CIN.
- Published
- 1996
- Full Text
- View/download PDF
22. [Standardize nomenclature for changes of the squamous epithelium].
- Author
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Angström T
- Subjects
- Female, Humans, Carcinoma, Squamous Cell pathology, Terminology as Topic, Uterine Cervical Neoplasms pathology
- Published
- 1994
23. Prostate cancer in northern Sweden. Incidence, survival and mortality in relation to tumour grade.
- Author
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Grönberg H, Bergh A, Damber JE, Jonsson H, Lenner P, and Angström T
- Subjects
- Aged, Aged, 80 and over, Humans, Incidence, Male, Middle Aged, Prostatic Neoplasms mortality, Survival Analysis, Survival Rate, Sweden epidemiology, Prostatic Neoplasms epidemiology, Prostatic Neoplasms pathology
- Abstract
In order to study changes in incidence, survival and mortality of prostate cancer in relation to morphological grade, we analysed all incident cases of prostate cancer in Northern Sweden during three 2-year periods (1974-1975, 1980-1981 and 1986-1987). The age-adjusted incidence increased by 35% from 1974-1975 to 1986-1987, but there was only a minor increase in the mortality rates. The increase in incidence was mainly due to well-differentiated (G1) and moderately differentiated (G2) tumours, whereas the incidence of poorly differentiated (G3) tumours remained stable. The 5-year relative survival rate increased significantly from 1974-1975 to 1986-1987. The relative survival rate for each tumour grade was, however, almost stable over the studied time period. After adjustment for tumour grade the differences in relative survival rate diminished. We believe that the most likely explanation for these changes in incidence, survival and mortality is enhanced detection of tumours with more favourable histology as a result of a more frequent use of the diagnostic tools available.
- Published
- 1994
- Full Text
- View/download PDF
24. Inhaled methacholine does not influence the cellular composition of bronchoalveolar lavage fluid.
- Author
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Söderberg M, Lundgren R, Bjermer L, and Angström T
- Subjects
- Administration, Inhalation, Adult, Bronchial Provocation Tests, Cell Count, Female, Humans, Male, T-Lymphocytes cytology, Bronchoalveolar Lavage Fluid cytology, Methacholine Chloride pharmacology
- Abstract
A methacholine or a histamine test is often used to quantitate bronchial responsiveness, in studies investigating the influence of various inhaled stimuli on the responsiveness and composition of bronchoalveolar lavage (BAL) fluid. Histamine has been shown to evoke an inflammatory cell response in BAL fluid. In order to test the influence of the methacholine test on the cellular composition of BAL fluid, we investigated 14 healthy, nonsmoking volunteers with a methacholine test 24 h before performing a BAL. The results showed no significant changes in the cellular composition of BAL fluid, as compared with control BAL in the same persons.
- Published
- 1993
- Full Text
- View/download PDF
25. Effect of cetirizine on histamine-induced bronchoconstriction and bronchoalveolar cells.
- Author
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Söderberg M, Lundgren R, and Angström T
- Subjects
- Adult, Bronchial Provocation Tests, Cell Count drug effects, Cetirizine, Double-Blind Method, Female, Forced Expiratory Volume drug effects, Humans, Hydroxyzine pharmacology, Male, Bronchoalveolar Lavage Fluid cytology, Bronchoconstriction drug effects, Histamine H1 Antagonists pharmacology, Hydroxyzine analogs & derivatives
- Abstract
The effect of the histamine H1 receptor antagonist (cetirizine) on histamine-induced bronchoconstriction and inflammatory cells in bronchoalveolar lavage fluid was studied in 12 healthy volunteers. In previous studies we have observed an increased number of inflammatory cells, albumin and hyaluronan in the bronchoalveolar lavage (BAL) fluid 24 h after an inhalation challenge test with histamine-chloride. In the present study certirizine blocked the histamine-induced bronchoconstriction but did not influence the cell counts in the bronchoalveolar lavage fluid. Our result suggests that the histamine-induced bronchoconstriction but not the recruitment of inflammatory cells in the BAL fluid is mediated by histamine H1 receptors.
- Published
- 1991
- Full Text
- View/download PDF
26. Inflammatory cell response in bronchoalveolar lavage fluid after nitrogen dioxide exposure of healthy subjects: a dose-response study.
- Author
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Sandström T, Stjernberg N, Eklund A, Ledin MC, Bjermer L, Kolmodin-Hedman B, Lindström K, Rosenhall L, and Angström T
- Subjects
- Adult, Cell Count, Dose-Response Relationship, Drug, Humans, Inflammation, Lymphocytes drug effects, Macrophages drug effects, Male, Mast Cells drug effects, Bronchoalveolar Lavage Fluid cytology, Environmental Exposure adverse effects, Nitrogen Dioxide adverse effects
- Abstract
The combination of environmental chamber exposure and bronchoalveolar lavage (BAL) was used to study the effects of the common air pollutant nitrogen dioxide (NO2). Eighteen healthy nonsmokers were exposed to NO2 during 20 min in an exposure chamber during light bicycle ergometer work. All subjects were examined with BAL at least 3 wks before exposure, as a reference. The subjects were re-examined with BAL, in groups of eight, 24 h after exposure to 4, 7 and 10 mg NO2.m.3 (2.25, 4.0 and 5.5 ppm), respectively. An inflammatory cell response was found after exposure to all concentrations. An increase in the number of lymphocytes in BAL fluid was observed after 7 and 10 mg.m.3 (p less than 0.05 and 0.02, respectively). An increase in the number of mast cells, that appears to be dose-dependent, was found after exposure to all concentrations. The proportion of lysozyme positive alveolar macrophages was elevated after exposure to 7 mg.m.3. The inflammatory mediators fibronectin, hyaluronan, angiotensin converting enzyme (ACE) and beta 2-microglobulin were unchanged by exposure. Due to the findings of inflammatory cell changes far below the peak exposure limits for work places in industrialized countries, 9-18 mg.m.3, the safety of these limits is questioned. Studies are in progress in our laboratory using BAL to evaluate the effects of repeated NO2 exposure.
- Published
- 1991
27. Static and flow cytometric DNA analysis compared to histologic prognostic factors in a cohort of stage T2 breast cancer.
- Author
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Arnerlöv C, Emdin SO, Roos G, Angström T, Bjersing L, Angquist KA, and Jonsson H
- Subjects
- Adenocarcinoma mortality, Adenocarcinoma pathology, Adenocarcinoma secondary, Breast Neoplasms mortality, Breast Neoplasms pathology, Female, Flow Cytometry, Follow-Up Studies, Humans, Lymphatic Metastasis, Multivariate Analysis, Neoplasm Staging, Ploidies, Prognosis, Survival Analysis, Adenocarcinoma genetics, Breast Neoplasms genetics, DNA, Neoplasm analysis
- Abstract
DNA analysis with static and flow cytometry was performed on archival smears and tissue sections in 99 patients with T2 breast cancer (Stage II). Tumour size, histologic grade and axillary node metastases were significant prognostic predictors. Static cytometry revealed 63% aneuploid tumours, and ploidy was significantly correlated to histologic grade and survival. DNA measurements obtained by static and flow cytometry were strongly correlated. According to flow cytometry 53% of the tumours were aneuploid. Flow cytometric DNA analysis correlated to histologic grade and survival and gave prognostic information among the lymph-node negative patients. Ploidy seems to be a significant, although not an independent prognostic indicator for T2 breast cancer.
- Published
- 1990
28. Effects of smoking and irradiated volume on inflammatory response in the lung of irradiated breast cancer patients evaluated with bronchoalveolar lavage.
- Author
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Bjermer L, Franzén L, Littbrand B, Nilsson K, Angström T, and Henriksson R
- Subjects
- Bronchoalveolar Lavage Fluid, Eosinophils pathology, Humans, Macrophages pathology, Neutrophils pathology, Breast Neoplasms radiotherapy, Pneumonia pathology, Radiation Injuries pathology, Smoking pathology
- Abstract
Quantitative measurements of the effects of irradiation on normal tissues in humans have been hard to obtain because most tissues are inaccessible and/or direct responses are difficult to quantify in a nondestructive manner. Pneumonitis and fibrotic lung disease are adverse effects seen in varying intensity in patients treated with radiotherapy for carcinomas of the thorax, e.g., breast cancer. In the present study the aim was to evaluate the inflammatory reaction in the underlying parenchyma following postoperative irradiation with bronchoalveolar lavage technique. Twenty-one patients (11 smokers, 10 nonsmokers) with breast cancer stage T1N0M0 received radiotherapy with photons to a target dose of 56 Gy following breast conservative surgery. Nineteen healthy controls were also included. The results showed a clear elevation of neutrophils, mast cells, eosinophils, and lymphocytes in the total irradiated groups, compared to controls. When subclassifying the material according to smoking habit, it was obvious that the smokers displayed a significantly decreased inflammatory reaction, i.e., reduced levels of mast cells and lymphocytes, compared to both nonsmoking controls and patients. Eosinophils were seen in an elevated number in all irradiated patients. Radiological signs of pneumonitis were observed in three patients, all in the nonsmoking group. No correlation was found between the volume of lung irradiated and the inflammatory response. It is concluded that bronchoalveolar lavage is a suitable and sensitive method for investigating radiotherapy-induced reactions in the human lung. Furthermore, ongoing smoking during the treatment depressed the inflammatory response in the lung parenchyma induced by irradiation. The present study as well as earlier observations justify further studies concerning the possibility of interaction of smoking with cancer treatment, both from the view of therapeutic failures and reduced adverse effects.
- Published
- 1990
29. Bronchoalveolar mastocytosis and lymphocytosis after nitrogen dioxide exposure in man: a time-kinetic study.
- Author
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Sandström T, Andersson MC, Kolmodin-Hedman B, Stjernberg N, and Angström T
- Subjects
- Adult, Animals, Atmosphere Exposure Chambers, Cell Count, Dose-Response Relationship, Drug, Humans, Male, Time Factors, Bronchoalveolar Lavage Fluid cytology, Lung drug effects, Lymphocytes cytology, Mast Cells cytology, Nitrogen Dioxide toxicity
- Abstract
The combination of environmental chamber exposure and bronchoalveolar lavage (BAL) was used to study the time-course of the cell response in the human lung to nitrogen dioxide (NO2). Healthy subjects were exposed for 20 min to 7 mg NO2.m-3 (4 ppm), a concentration which occurs indoors in industries and is below the peak exposure limit for work places in most countries, 10 mg.m-3 (5.5 ppm). BAL was performed in all subjects several weeks before exposure and 4, 8, 24 and 72 h after exposure, in eight subjects at each time. Mastocytosis and lymphocytosis were found in BAL fluid 4-24 h after exposure, with normalization after 72 h. A mild increase in lysozyme positive macrophages was found 24-72 h after exposure. The time-course of the human pulmonary cell response to NO2, demonstrated in BAL fluid, represents a new and previously not reported finding after exposure to this common air pollutant. Our findings are diverging from results obtained in animal studies, using approximately the same NO2 concentrations, indicating that the results from the animal studies may not be transferable to man.
- Published
- 1990
30. Fine-needle aspiration biopsy of pancreatic masses.
- Author
-
Athlin L, Blind PJ, and Angström T
- Subjects
- Adult, Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Pancreatic Neoplasms mortality, Pancreatic Neoplasms pathology, Prognosis, Retrospective Studies, Sensitivity and Specificity, Survival Rate, Ultrasonography, Biopsy, Needle, Pancreatic Neoplasms diagnosis
- Abstract
The results of fine-needle aspiration biopsy of pancreatic masses in 79 patients (percutaneous with ultrasonic guidance in 23 and peroperative in 56) were evaluated and correlated to survival (follow-up at least 2 years). The original biopsy diagnosis was malignancy in 41 patients, histologically confirmed in 19, all but two of whom died of cancer within 18 months. None of the 22 patients without histologic verification of the primary malignant cytodiagnosis survived for 18 months. The fine-needle biopsy showed benign cells in 30 patients, in 13 of whom histologic diagnosis was obtained, revealing carcinoma in seven. Six of these seven died within a year, but of the six with histologically benign lesion, five survived for more than 2 years. All 17 patients without histologic verification of benign aspiration biopsy findings survived more than 24 months. The biopsy diagnosis was inconclusive in eight patients. Four of them proved to have carcinoma and died within 18 months. The sensitivity of fine-needle aspiration biopsy of the pancreas was 76% in this study and the predictive value for malignancy was 100%.
- Published
- 1990
31. [The initial stages of cervical cancer--revived problems of nomenclature and definition].
- Author
-
Bjersing L and Angström T
- Subjects
- Female, Humans, Terminology as Topic, Precancerous Conditions pathology, Uterine Cervical Neoplasms pathology
- Published
- 1984
32. Familial occurrence of histiocytosis.
- Author
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Frisell E, Björkstén B, Holmgren G, and Angström T
- Subjects
- Consanguinity, Female, Genes, Recessive, Humans, Infant, Male, Pedigree, Sweden, Histiocytosis, Langerhans-Cell genetics
- Abstract
The clinical and histological findings of four children with lethal histiocytosis are reported. The children belonged to two sibships and originated from a Swedish geographical isolate. Consanguinity between the parents was established for one of the sibships, belonging to a pedigree in which malignant disease occurred in two generations. The observations indicate that the type of histiocytosis investigated is caused by homozygosity for a single recessive gene. The association between histiocytosis and malignancy is discussed.
- Published
- 1977
- Full Text
- View/download PDF
33. Bone marrow examination as a diagnostic tool in carcinoma of the prostate.
- Author
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Duchek M, Lingardh G, Saterborg NE, Winblad B, and Angström T
- Subjects
- Adenocarcinoma diagnostic imaging, Adenocarcinoma pathology, Aged, Bone Marrow pathology, Bone Marrow Cells, Bone Neoplasms diagnostic imaging, Bone Neoplasms pathology, Humans, Male, Middle Aged, Neoplasm Metastasis, Radiography, Adenocarcinoma diagnosis, Bone Marrow Examination, Bone Neoplasms diagnosis, Prostatic Neoplasms
- Abstract
155 patients with a newly discovered carcinoma of the prostate were investigated for the presence of bone metastases. Straight X-ray (conventional X-ray) revealed such secondary in 38 cases and bone marrow examination in 20. Three patients with a normal X-ray picture nevertheless showed cancer cells on bone marrow examination. The authors consider bone marrow examination as a valuable complement to the routine invedtigation of the patients with carcinoma of the prostate.
- Published
- 1975
- Full Text
- View/download PDF
34. Comparison of transbronchial fine needle aspiration biopsy, aspiration of bronchial secretion, bronchial washing, brush biopsy and forceps biopsy in the diagnosis of lung cancer.
- Author
-
Lundgren R, Bergman F, and Angström T
- Subjects
- Adult, Aged, Biopsy methods, Biopsy, Needle, Bronchoscopy, Female, Humans, Lung Neoplasms pathology, Male, Middle Aged, Suction, Therapeutic Irrigation, Bronchi metabolism, Lung Neoplasms diagnosis
- Abstract
The accuracy of morphologic diagnosis from transbronchial fine needle aspirates (TBN), bronchial secretions (BS), bronchial washings (BW), brush biopsy (BB) and forceps biopsy (FB) sampled via a flexible fiberoptic bronchoscope, was studied in 60 malignant lung tumours. In visible tumours the best results were obtained with FB (85%) while TBN was positive in 65%. In cases were bronchoscopy only showed secondary signs of tumour, the sensitivity of FB, BB and TBN was higher than that of BW and aspiration of BS. In tumours not visible through the bronchoscope, the diagnostic accuracy of BB, BW and aspiration of BS was higher than that of FB and TBN. In the diagnosis of carcinoma of the lung the diagnostic accuracy of combining FB and one cytologic method (BW) was higher than that of any of the single methods (p less than 0.05) while no appreciable increase was achieved by using additional methods.
- Published
- 1983
35. Cytological and immunochemical characterization of middle ear effusions: an experimental approach.
- Author
-
Stenfors LE, Hellström S, Angström T, and Johansson U
- Subjects
- Animals, Blood Proteins analysis, Exudates and Transudates pathology, Otitis Media with Effusion pathology, Otitis Media, Suppurative pathology, Rats, Exudates and Transudates analysis, Otitis Media metabolism, Otitis Media with Effusion metabolism, Otitis Media, Suppurative metabolism
- Abstract
The aim of the present study was to experimentally provoke well-defined serous and purulent effusion materials and to analyze the fluid using cytological and immunochemical techniques. Using these parameters an attempt was made to find a marker(s) for the degree of inflammation, duration of the disease and possible sequelae.
- Published
- 1985
- Full Text
- View/download PDF
36. Cell response in bronchoalveolar lavage fluid after exposure to sulfur dioxide: a time-response study.
- Author
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Sandström T, Stjernberg N, Andersson MC, Kolmodin-Hedman B, Lundgren R, Rosenhall L, and Angström T
- Subjects
- Adult, Bronchi drug effects, Bronchi pathology, Bronchoscopy, Cell Count, Humans, Lymphocytes pathology, Macrophages metabolism, Macrophages pathology, Male, Mast Cells pathology, Monocytes metabolism, Monocytes pathology, Muramidase metabolism, Time Factors, Bronchoalveolar Lavage Fluid cytology, Sulfur Dioxide adverse effects
- Abstract
Bronchoalveolar lavage (BAL) has been performed in 22 healthy nonsmoking male volunteers after exposure to 8 ppm SO2 (20 mg/m3). The exposure level exceeds the US Short-Term Exposure Limit (STEL) of 5 ppm, but occurs as peak exposures in industrial indoor environments. Exposures were made during light work on a bicycle ergometer in an environmental exposure chamber for 20 min. BAL was performed 2 wk or more before exposure and 4, 8, 24, and 72 h after exposure in eight subjects at each time interval. Four hours after exposure significant increases were found in the numbers of lysozyme-positive macrophages, lymphocytes, and mast cells (p less than 0.02 to 0.05). Lymphocytes, lysozyme-positive macrophages, total count of alveolar macrophages, and total cell number increased to peak values 24 h after exposure (p less than 0.02 to 0.05). Seventy-two hours after exposure the cell numbers and distribution had returned to normal. The time course of the cell reactions found in BAL fluid after controlled SO2 exposure represents a new and previously not reported response to a noxious gas.
- Published
- 1989
- Full Text
- View/download PDF
37. Transvaginal and transrectal aspiration biopsy in diagnosis and classification of ovarian tumours.
- Author
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Kjellgren O and Angström T
- Subjects
- Carcinoma diagnosis, Cystadenoma diagnosis, Diagnosis, Differential, Dysgerminoma diagnosis, Endometriosis diagnosis, Female, Fibroma diagnosis, Granulosa Cell Tumor diagnosis, Humans, Neoplasm Metastasis, Teratoma diagnosis, Thecoma diagnosis, Biopsy, Needle, Ovarian Cysts diagnosis, Ovarian Neoplasms diagnosis
- Published
- 1979
38. Studies on a feminizing ovarian mesenchymoma (granulosa cell tumor). I. Aspiration biopsy cytology, histology, and ultrastructure.
- Author
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Bjersing L, Frankendal B, and Angström T
- Subjects
- Aged, Biopsy, Needle, Estrogens metabolism, Female, Granulosa Cell Tumor physiopathology, Humans, Microscopy, Electron, Ovarian Neoplasms physiopathology, Paraneoplastic Endocrine Syndromes, Granulosa Cell Tumor pathology, Ovarian Neoplasms pathology, Ovary pathology
- Published
- 1973
- Full Text
- View/download PDF
39. Prognostic factors in locally advanced breast cancer (T3, T4) with special reference to tumor cell DNA content.
- Author
-
Arnerlöv C, Emdin SO, Roos G, Angström T, Bjersing L, Angquist KA, Larsson LG, and Jonsson H
- Subjects
- Breast Neoplasms drug therapy, Breast Neoplasms radiotherapy, Breast Neoplasms surgery, Female, Follow-Up Studies, Humans, Lymphatic Metastasis, Mastectomy, Neoplasm Staging, Prognosis, Tamoxifen therapeutic use, Biomarkers, Tumor analysis, Breast Neoplasms pathology, DNA, Neoplasm analysis
- Abstract
The prognostic value of DNA analysis was studied retrospectively in 91 patients with locally advanced breast cancer (T3, T4) and a follow-up time of 3-7 years. Tumor cell DNA analysis was performed by static cytometry on aspiration biopsy specimens in 42 cases and on tissue sections in 49 cases. The tumors were classified as euploid or aneuploid. Sixty-four percent of the tumors were aneuploid. DNA pattern correlated significantly to histologic grade and axillary perinodal growth and also to survival. DNA pattern, histologic grade and axillary node metastases correlated significantly to disease-free survival (DFS). In this series of patients with locally advanced breast cancer DNA determinations gave important prognostic information.
- Published
- 1988
- Full Text
- View/download PDF
40. Cytology and cytochemistry of acinic cell carcinoma.
- Author
-
Franzén L, Carlsöö B, and Angström T
- Subjects
- Acid Phosphatase analysis, Adolescent, Adult, Aged, Amylases analysis, Biopsy, Needle, Carcinoma analysis, Carcinoma ultrastructure, Cyclic AMP analysis, Female, Histocytochemistry, Humans, Male, Middle Aged, Organoids ultrastructure, Parotid Neoplasms analysis, Parotid Neoplasms ultrastructure, Staining and Labeling, Carcinoma pathology, Parotid Neoplasms pathology
- Abstract
In a primary material of 314 epithelial parotid tumours treated at the University Hospital in Umeå since 1958, fiften (11 female and 4 male patients) were classified as acinic cell carcinomas. Four of the female patients were in the age range 16--19 years. At the ultrastructural level, granulated cells were the predominant cell type in four tumours studied. Agranulated cells highly reminiscent of intercalated duct cells were also encountered, however. Various cytochemical techniques were employed to demonstrate periodate reactive carbohydrates and acid phosphatase activity. Tumour specimens were also collected and analysed for their content of amylase and cyclic AMP. The cyto- and biochemical findings are discussed and correlated to those observed in normal salivary gland tissue.
- Published
- 1979
- Full Text
- View/download PDF
41. Cytologic findings in vaginal and oral smears from pregnant women.
- Author
-
Hugoson A, Winberg E, and Angström T
- Subjects
- Adult, Cell Count, Cell Nucleus, Female, Humans, Postpartum Period, Statistics as Topic, Time Factors, Mouth Mucosa cytology, Pregnancy, Vaginal Smears
- Published
- 1972
42. Tumour cells in peritoneal rinsing fluid.
- Author
-
Lindqvist B, Michaelson G, Säterborg NE, and Angström T
- Subjects
- False Positive Reactions, Humans, Peritoneal Cavity pathology, Peritoneal Neoplasms diagnosis, Uremia pathology, Peritoneal Dialysis, Peritoneal Neoplasms pathology
- Published
- 1969
- Full Text
- View/download PDF
43. A diagnostic and follow-up program for patients in the 20-34 year age group with suspicious cervical smears.
- Author
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Bjersing L, Holmberg NG, Lundström P, Magnússon SS, Segerbrand E, and Angström T
- Subjects
- Adult, Biopsy, Carcinoma pathology, Carcinoma surgery, Cervix Uteri pathology, Cervix Uteri surgery, Colposcopy, Female, Humans, Uterine Cervical Neoplasms pathology, Uterine Cervical Neoplasms surgery, Vaginal Smears, Carcinoma diagnosis, Precancerous Conditions diagnosis, Uterine Cervical Neoplasms diagnosis
- Published
- 1971
44. [Aspiration biopsy in cytological diagnosis of ovarian neoplasms].
- Author
-
Dahlgren S, Kjellgren O, and Angström T
- Subjects
- Adolescent, Adult, Aged, Biopsy, Child, Cytodiagnosis instrumentation, Female, Humans, Inhalation, Methods, Middle Aged, Ovary pathology, Time Factors, Ovarian Neoplasms diagnosis
- Published
- 1968
45. Cytologic findings in vaginal and oral smears from pregnant women.
- Author
-
Hugoson A, Winberg E, and Angström T
- Subjects
- Adult, Cheek cytology, Female, Humans, Postpartum Period, Pregnancy, Vaginal Smears, Gingiva cytology, Gonadal Steroid Hormones, Mouth Mucosa cytology, Vagina cytology
- Published
- 1971
46. Fine-needle aspiration biopsy in diagnosis, and classification of ovarian carcinoma.
- Author
-
Kjellgren O, Angström T, Bergman F, and Wiklund DE
- Subjects
- Adenocarcinoma diagnosis, Adolescent, Adult, Aged, Child, Cystadenocarcinoma diagnosis, Cystadenoma diagnosis, Diagnosis, Differential, Diagnostic Errors, Endometriosis diagnosis, Female, Fibroma diagnosis, Humans, Leiomyoma diagnosis, Mesonephroma diagnosis, Methods, Middle Aged, Needles, Ovarian Cysts diagnosis, Ovarian Neoplasms epidemiology, Ovarian Neoplasms pathology, Ovarian Neoplasms therapy, Sweden, Biopsy, Cytodiagnosis, Ovarian Neoplasms diagnosis
- Published
- 1971
- Full Text
- View/download PDF
47. Carcinoid tumour in the benign cystic teratoma of the ovary.
- Author
-
Falkmer S, Frankendal B, Hassler O, and Angström T
- Subjects
- Aged, Biopsy, Carcinoid Tumor diagnosis, Dermoid Cyst diagnosis, Female, Humans, Ovarian Neoplasms diagnosis, Carcinoid Tumor complications, Dermoid Cyst complications, Ovarian Cysts complications, Ovarian Neoplasms complications
- Published
- 1972
48. [Curative effect of directed probatory excision in cancer in situ colli uteri in age group 20-34 years (preliminary report)].
- Author
-
Bjersing L, Holmberg NG, Magnusson S, Lundström P, and Angström T
- Subjects
- Adult, Biopsy, Female, Humans, Uterine Cervical Neoplasms diagnosis, Uterine Cervical Neoplasms surgery
- Published
- 1970
49. Cryptococcosis in Sweden.
- Author
-
Bergman F, Brun A, d' Elia G, Wallerström A, and Angström T
- Subjects
- Basal Ganglia pathology, Cerebellar Cortex pathology, Female, Humans, Kidney pathology, Liver Cirrhosis complications, Lymph Nodes pathology, Middle Aged, Myocarditis complications, Subarachnoid Space pathology, Sweden, Brain pathology, Cryptococcosis pathology, Meningoencephalitis pathology
- Published
- 1967
- Full Text
- View/download PDF
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