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7 results on '"Bet v 1‐like"'

1. Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut.

Author

Bastiaan‐Net, Shanna, Pina‐Pérez, Maria C., Dekkers, Bas J. W., Westphal, Adrie H., America, Antoine H. P., Ariëns, Renata M. C., Jong, Nicolette W., Wichers, Harry J., and Mes, Jurriaan J.

Abstract

Proteins from cashew nut can elicit mild to severe allergic reactions. Three allergenic proteins have already been identified, and it is expected that additional allergens are present in cashew nut. pathogenesis‐related protein 10 (PR10) allergens from pollen have been found to elicit similar allergic reactions as those from nuts and seeds. Therefore, we investigated the presence of PR10 genes in cashew nut. Using RNA‐seq analysis, we were able to identify several PR10‐like transcripts in cashew nut and clone six putative PR10 genes. In addition, PR10 protein expression in raw cashew nuts was confirmed by immunoblotting and liquid chromatography–mass spectrometry (LC–MS/MS) analyses. An in silico allergenicity assessment suggested that all identified cashew PR10 proteins are potentially allergenic and may represent three different isoallergens. [ABSTRACT FROM AUTHOR]

Published
2020
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2. Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut

Author

Maria Consuelo Pina-Pérez, Harry J. Wichers, Shanna Bastiaan-Net, Jurriaan J. Mes, Renata M.C. Ariëns, Adrie H. Westphal, Antoine H. P. America, Nicolette W. de Jong, Bas J.W. Dekkers, and Internal Medicine

Subjects
Bioinformatics analysis, Full‐length Papers, In silico, Biochemie, RNA-Seq, Biology, Biochemistry, Protein expression, Article, oral allergy syndrome (OAS), 03 medical and health sciences, Tandem Mass Spectrometry, Levensmiddelenchemie, Nuts, Anacardium, Computer Simulation, Laboratorium voor Plantenfysiologie, Cashew nut, Molecular Biology, Gene, PR10, 030304 developmental biology, Plant Proteins, VLAG, Food, Health & Consumer Research, 0303 health sciences, Food Chemistry, 030302 biochemistry & molecular biology, digestive, oral, and skin physiology, food and beverages, Allergens, cashew nut, Health & Consumer Research, Food, Anacardium occidentale, in silico allergenicity analysis, BIOS Applied Metabolic Systems, RNA‐seq, Bet v 1‐like, EPS, RNA-seq, Laboratory of Plant Physiology, Bet v 1-like, Chromatography, Liquid
Abstract

Proteins from cashew nut can elicit mild to severe allergic reactions. Three allergenic proteins have already been identified, and it is expected that additional allergens are present in cashew nut. pathogenesis‐related protein 10 (PR10) allergens from pollen have been found to elicit similar allergic reactions as those from nuts and seeds. Therefore, we investigated the presence of PR10 genes in cashew nut. Using RNA‐seq analysis, we were able to identify several PR10‐like transcripts in cashew nut and clone six putative PR10 genes. In addition, PR10 protein expression in raw cashew nuts was confirmed by immunoblotting and liquid chromatography–mass spectrometry (LC–MS/MS) analyses. An in silico allergenicity assessment suggested that all identified cashew PR10 proteins are potentially allergenic and may represent three different isoallergens.

Published
2020
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3. Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut

Author

Bastiaan-Net, S. (Shanna), Pina-Pérez, M.C. (Maria C.), Dekkers, B.J.W. (Bas J. W.), Westphal, A.H. (Adrie H.), America, A.H.P. (Antoine H. P.), Ariëns, R.M.C. (Renata M. C.), Jong, N.W. (Nicolette) de, Wichers, H.J., Mes, J.J. (Jurriaan J.), Bastiaan-Net, S. (Shanna), Pina-Pérez, M.C. (Maria C.), Dekkers, B.J.W. (Bas J. W.), Westphal, A.H. (Adrie H.), America, A.H.P. (Antoine H. P.), Ariëns, R.M.C. (Renata M. C.), Jong, N.W. (Nicolette) de, Wichers, H.J., and Mes, J.J. (Jurriaan J.)

Abstract

Proteins from cashew nut can elicit mild to severe allergic reactions. Three allergenic proteins have already been identified, and it is expected that additional allergens are present in cashew nut. pathogenesis-related protein 10 (PR10) allergens from pollen have been found to elicit similar allergic reactions as those from nuts and seeds. Therefore, we investigated the presence of PR10 genes in cashew nut. Using RNA-seq analysis, we were able to identify several PR10-like transcripts in cashew nut and clo

Published
2020
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4. Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut

Author

Bastiaan-Net, Shanna, Pina-Pérez, Maria C., Dekkers, Bas J.W., Westphal, Adrie H., America, Antoine H.P., Ariëns, Renata M.C., de Jong, Nicolette W., Wichers, Harry J., Mes, Jurriaan J., Bastiaan-Net, Shanna, Pina-Pérez, Maria C., Dekkers, Bas J.W., Westphal, Adrie H., America, Antoine H.P., Ariëns, Renata M.C., de Jong, Nicolette W., Wichers, Harry J., and Mes, Jurriaan J.

Abstract

Proteins from cashew nut can elicit mild to severe allergic reactions. Three allergenic proteins have already been identified, and it is expected that additional allergens are present in cashew nut. pathogenesis-related protein 10 (PR10) allergens from pollen have been found to elicit similar allergic reactions as those from nuts and seeds. Therefore, we investigated the presence of PR10 genes in cashew nut. Using RNA-seq analysis, we were able to identify several PR10-like transcripts in cashew nut and clone six putative PR10 genes. In addition, PR10 protein expression in raw cashew nuts was confirmed by immunoblotting and liquid chromatography–mass spectrometry (LC–MS/MS) analyses. An in silico allergenicity assessment suggested that all identified cashew PR10 proteins are potentially allergenic and may represent three different isoallergens.

Published
2020

5. Kiwifruit Act d 11 is the first member of the ripening-related protein family identified as an allergen.

Author

D'Avino, R., Bernardi, M. L., Wallner, M., Palazzo, P., Camardella, L., Tuppo, L., Alessandri, C., Breiteneder, H., Ferreira, F., Ciardiello, M. A., and Mari, A.

Subjects
*ALLERGENS, *KIWIFRUIT, *FOOD allergy, *AMINO acid sequence, *IMMUNOBLOTTING, *THAUMATINS, *SERUM albumin
Abstract

Background: Kiwifruit is an important cause of food allergy. A high amount of a protein with a molecular mass compatible with that of Bet v 1 was observed in the kiwifruit extract. Objective: To identify and characterize kirola, the 17-kDa protein of green kiwifruit (Act d 11). Methods: Act d 11 was purified from green kiwifruit. Its primary structure was obtained by direct protein sequencing. The IgE binding was investigated by skin testing, immunoblotting, inhibition tests, and detection by the ISAC microarray in an Italian cohort and in selected Bet v 1-sensitized Austrian patients. A clinical evaluation of kiwi allergy was carried out. Results: Act d 11 was identified as a member of the major latex protein/ripeningrelated protein (MLP/RRP) family. IgE binding to Act d 11 was shown by all the applied testing. Patients tested positive for Act d 11 and reporting symptoms on kiwifruit exposure were found within the Bet v 1-positive subset rather than within the population selected for highly reliable history of allergic reactions to kiwifruit. Epidemiology of Act d 11 IgE reactivity was documented in the two cohorts. IgE co-recognition of Act d 11 within the Bet v 1-like molecules is documented using the microarray IgE inhibition assay. Conclusions: Act d 11 is the first member of the MLP/RRP protein family to be described as an allergen. It displays IgE co-recognition with allergens belonging to the PR-10 family, including Bet v 1. [ABSTRACT FROM AUTHOR]

Published
2011
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6. Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut.

Author

Bastiaan-Net S, Pina-Pérez MC, Dekkers BJW, Westphal AH, America AHP, Ariëns RMC, de Jong NW, Wichers HJ, and Mes JJ

Subjects
Chromatography, Liquid, Tandem Mass Spectrometry, Allergens biosynthesis, Allergens chemistry, Allergens genetics, Anacardium chemistry, Anacardium genetics, Anacardium metabolism, Computer Simulation, Nuts chemistry, Nuts genetics, Nuts metabolism, Plant Proteins biosynthesis, Plant Proteins chemistry, Plant Proteins genetics, RNA-Seq
Abstract

Proteins from cashew nut can elicit mild to severe allergic reactions. Three allergenic proteins have already been identified, and it is expected that additional allergens are present in cashew nut. pathogenesis-related protein 10 (PR10) allergens from pollen have been found to elicit similar allergic reactions as those from nuts and seeds. Therefore, we investigated the presence of PR10 genes in cashew nut. Using RNA-seq analysis, we were able to identify several PR10-like transcripts in cashew nut and clone six putative PR10 genes. In addition, PR10 protein expression in raw cashew nuts was confirmed by immunoblotting and liquid chromatography-mass spectrometry (LC-MS/MS) analyses. An in silico allergenicity assessment suggested that all identified cashew PR10 proteins are potentially allergenic and may represent three different isoallergens., (© 2020 The Authors. Protein Science published by Wiley Periodicals, Inc. on behalf of The Protein Society.)

Published
2020
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7. A transcriptomic approach to food safety - Fruit allergens and mycotoxins

Author

Botton, Alessandro

Subjects
food allergy, non-specific Lipid Transfer proteins, agricultural practices, hypoallergenic fruits, Aspergillus carbonarius, food allergy, fruit allergens, Bet v 1-like, non-specific Lipid Transfer proteins, Thaumatin-like proteins, profilins, environmental factors, genetic factors, hypoallergenic fruits, agricultural practices, mycotoxins, ochratoxin A, Aspergillus carbonarius, OTA biosynthesis regulation, profilins, Settore AGR/03 - Arboricoltura Generale e Coltivazioni Arboree, fruit allergens, environmental factors, mycotoxins, Thaumatin-like proteins, genetic factors, OTA biosynthesis regulation, ochratoxin A, Bet v 1-like
Published
2008

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