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Abstract Interstitial cystitis/bladder pain syndrome (IC/BPS) is a complex chronic pain disorder with an elusive etiology and nonspecific symptoms. Although numerous animal models with phenotypes similar to human disease have been established, no available regimen can consistently alleviate clinical symptoms. This dilemma led us to question whether current animal models adequately represent IC/BPS. We compared four commonly used IC/BPS rat models to determine their diverse histopathological and molecular patterns. Female rats were given single treatments with hydrochloric acid (HCL), acetic acid (AA), protamine sulfate plus lipopolysaccharide (PS + LPS), or cyclophosphamide (CYP) to induce IC/BPS. Bladder sections were stained for histopathologic evaluation, and mRNA expression profiles were examined using next-generation sequencing and gene set analyses. Mast cell counts were significantly higher in the HCL and AA groups than in the PS + LPS, CYP, and control groups, but only the AA group showed significant collagen accumulation. The models differed substantially in terms of their gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our observations suggest that none of these rat models fully reflects the complexity of IC/BPS. We recommend that future studies apply and compare multiple models simultaneously to fully replicate the complicated features of IC/BPS.

Microfluidic is a technology that allows the precise control of fluid in a micro-channel. With its advantages of high throughput and low cost, microfluidic technology has achieved good performance in various fields in recent years. Arthritis is a general term for a variety of joint diseases, which can be clinically manifested as joint pain and swelling, seriously affecting people's physical and mental health. At present, the causes of arthritis disease are still unknown, and existing disease models and treatment methods are still limited, so more treatments need to be developed. Microfluidic organ chip is a cutting-edge technology to build a bionic human organ model, which can reflect the structure and function characteristics of human organs by simulating the physiological environment of tissues and cells in vitro. This paper reviews the application of microfluidic technology in the modeling and treatment of arthritis, hoping to open up a new vision for the study of arthritis.

The phenomenon of fine particle migration through the voids of the granule skeleton under the seepage force is called suffusion. Relative density, original fine particle content, and gap ratio are thought to play vital roles in the suffusion process. This paper investigates the effect of geometrical characteristics (i.e., original fine particle content, gap ratio, and relative density) on soil structure and mechanical performance (i.e., small strain shear modulus) using the bender element method technique. The small strain shear modulus (G0) is used as a mechanical parameter to evaluate the shear stress transmission of the soil structure along with the erosion process. The comparison between erosion percentage and vertical strain change suggests the alteration in soil fabric after soil erosion. The G0 monitoring results show that packings with a higher original fine particle content have a lower G0 value, whereas the gap ratio and relative density present a positive relationship with G0.

Abstract Effective drugs for the treatment of gastric cancer (GC) are still lacking. Nortriptyline Hydrochloride (NTP), a commonly used antidepressant medication, has been demonstrated by numerous studies to have antitumor effects. This study first validated the ability of NTP to inhibit GC and preliminarily explored its underlying mechanism. To begin with, NTP inhibits the activity of AGS and HGC27 cells (Human-derived GC cells) in a dose-dependent manner, as well as proliferation, cell cycle, and migration. Moreover, NTP induces cell apoptosis by upregulating BAX, BAD, and c-PARP and downregulating PARP and Bcl-2 expression. Furthermore, the mechanism of cell death caused by NTP is closely related to oxidative stress. NTP increases intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) levels, decreasing the mitochondrial membrane potential (MMP) and inducing glucose (GSH) consumption. While the death of GC cells can be partially rescued by ROS inhibitor N-acetylcysteine (NAC). Mechanistically, NTP activates the Kelch-like ECH-associated protein (Keap1)—NF-E2-related factor 2 (Nrf2) pathway, which is an important pathway involved in oxidative stress. RNA sequencing and proteomics analysis further revealed molecular changes at the mRNA and protein levels and provided potential targets and pathways through differential gene expression analysis. In addition, NTP can inhibited tumor growth in nude mouse subcutaneous tumor models constructed respectively using AGS and MFC (mouse-derived GC cells), providing preliminary evidence of its effectiveness in vivo. In conclusion, our study demonstrated that NTP exhibits significant anti-GC activity and is anticipated to be a candidate for drug repurposing.

Abstract Background Green tea has been reported to be potentially protective against the development of cardiovascular disease (CVD). This study aimed to investigate the association between green tea consumption and incident CVD in type 2 diabetes (T2D) patients with overweight/obesity. Methods A total of 4756 Chinese overweight/obese T2D patients were recruited and followed up for 6.27 years. Information on green tea consumption was collected at baseline using interviewer-administered questionnaires. Hazard ratios (HRs) and 95% confidence intervals (CIs) for incident CVD according to green tea consumption were estimated using the Cox proportional hazards model. Results Compared with non-habitual consumers, participants who consumed > 5 g/day of green tea leaves reduced the risk of CVD by 29% (95%CI: 0.55–0.92), stroke by 30% (95%CI: 0.51–0.95) and coronary heart disease (CHD) by 40% (95%CI: 0.40–0.89). Similarly, participants who consumed green tea for ≥ 40 years reduced the risk of CVD by 31% (95%CI: 0.54–0.88), stroke by 33% (95%CI: 0.50–0.90) and CHD by 39% (95%CI: 0.42–0.88). Among participants with 5 g/day of tea leaves and > 40 years of tea consumption were associated with 59% (95%CI: 0.23–0.72) and 57% (95%CI: 0.26–0.74) reduced risk of stroke, respectively. However, among participants with ≥ 5-year history of T2D, > 5 g/day of tea leaves and > 40 years of tea consumption were associated with a 50% (95%CI: 0.30–0.82) and 46% (95%CI: 0.35–0.85) reduced risk of CHD, respectively. Conclusions Green tea consumption is associated with reduced risk of CVD, stroke, and CHD in overweight/obese T2D patients.

Summary: CD8+ T cells are rendered exhausted in tumor and chronic infection. Among heterogeneous exhausted T cells, a subpopulation of progenitor-like (Tpex) cells have been found important for long-term tumor or pathogen control and are also the main responders in immunotherapy. Using an RFP reporter mouse for the orphan nuclear receptor NR4A1, originally characterized as critical in T cell dysfunction, we discover that the reporter is highly expressed in Tpex cells in tumor and chronic infection. Enforced expression of Nr4a1 promotes Tpex cell accumulation, whereas tumor control is improved after Nr4a1 deletion, associated with increased effector function but decreased long-term maintenance of CD8+ T cells. Integrating chromatin immunoprecipitation sequencing (ChIP-seq) and RNA sequencing (RNA-seq) analysis, NR4A1 is found to bind and promote the expression of Tpex-related genes, as well as suppress terminal differentiation-associated genes. This study therefore has identified a key role of NR4A1 in Tpex regulation and provides a promising target for immunotherapy.

BackgroundIt is important to figure out the immunity of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) reinfection to understand the response of humans to viruses. A serological survey for previously infected populations in Jiangsu Province was conducted to compare the antibody level of SARS-CoV-2 in reinfection by Omicron or not.MethodsReinfection with SARS-CoV-2 was defined as an individual being infected again after 90 days of the initial infection. Telephone surveys and face-to-face interviews were implemented to collect information. Experimental and control serum samples were collected from age-sex-matched reinfected and non-reinfected cases, respectively. IgG anti-S and neutralizing antibodies (Nab) concentrations were detected by the Magnetism Particulate Immunochemistry Luminescence Method (MCLIA). Antibody titers were log(2)-transformed and analyzed by a two-tailed Mann–Whitney U test. Subgroup analysis was conducted to explore the relationship between the strain type of primary infection, SARS-Cov-2 vaccination status, and antibody levels. Multivariate linear regression models were used to identify associations between reinfection with IgG and Nab levels.ResultsSix hundred thirty-one individuals were enrolled in this study, including 327 reinfected cases and 304 non-reinfected cases. The reinfection group had higher IgG (5.65 AU/mL vs. 5.22 AU/mL) and Nab (8.02 AU/mL vs. 7.25 AU/mL) levels compared to the non-reinfection group (p

We aimed to investigate the improvement effect of Beclin-1 on the cognitive function of mice with Alzheimer"s disease (AD). Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were isolated from neonatal umbilical cord tissues. The cells stably overexpressing Beclin-1 were constructed, and those of passages 5, 10, and 15 were used. Forty-eight AD mice were randomly divided into AD, P5 hUC-MSCs, P15 hUC-MSCs (P15MSCs) and OE Beclin 1-P15 hUC-MSCs (OE Bec-P15MSCs) groups (n=12). Overexpressing Beclin-1 significantly enhanced the proliferation and migration of MSCs of different passages, and reduced G0/G1 arrest. The OE Bec-P15MSCs group had the longest total distance, the shortest time in forced swimming test, and the largest total food consumption in novelty suppressed feeding test. Transplanting hUC-MSCs and overexpressing Beclin-1 significantly reduced Aβ deposition. The expressions of P-tau (Ser396), P-tau (Ser231) and P-tau (Ser235) were significantly inhibited by hUC-MSCs transplantation and Beclin-1 overexpression, especially in the OE Bec- P15MSCs group. Double positive staining of EdU+/DCX+ cells, EdU+/NeuN+ cells and EdU+/Nestin+ cells significantly increased in the OE Bec-P15MSCs group compared to those in other groups (P

Abstract Background In professional ethics-related events, there are various unpleasant and complex ethical issues that require strong moral courage. Our aim is to identify and describe the potential categories of moral courage among nurses and to clarify the coping styles of nurses under different categories. Method A cross-sectional study was conducted using three data collection tools: a self-designed general information questionnaire, a Chinese version of the Moral Courage Scale, and a Trait Coping Style Questionnaire. Three hundred fourteen nurses from a tertiary hospital in Heilongjiang Province, China, were analysed using potential profile analysis, descriptive analysis, and Mann-Whitney U test data. Result The latent profile analysis (LPA) results indicate that the two-profile model is the most suitable and supports the existence of two different moral courage profiles: the low moral courage group (60.51%) and the high moral courage group (39.49%), with a high relative entropy value (0.922). The results point to a good profile solution, and there are significant differences between the two profiles. The Mann-Whitney U-test results showed that the positive coping scores of the high moral courage group were significantly higher than those of the low moral courage group, and the negative coping scores of the high moral courage group were significantly lower than those of the low moral courage group. Conclusion Our results reveal the heterogeneity of moral courage in the nurse sample and indicate that nurses in the high moral courage group tend to choose positive coping styles, while nurses in the low moral courage group are more likely to develop negative coping emotions. This provides important significance and reference value for nursing managers, who can propose customised management plans based on the types of moral courage of the nursing community and the coping styles under different categories.

Summary: Preeclampsia (PE) is a hypertensive pregnancy disorder with increased risk of maternal and fetal morbidity and mortality. Abnormal extravillous trophoblast (EVT) development and function is considered to be the underlying cause of PE, but has not been previously modeled in vitro. We previously derived induced pluripotent stem cells (iPSCs) from placentas of PE patients and characterized abnormalities in formation of syncytiotrophoblast and responses to changes in oxygen tension. In this study, we converted these primed iPSC to naïve iPSC, and then derived trophoblast stem cells (TSCs) and EVT to evaluate molecular mechanisms underlying PE. We found that primed (but not naïve) iPSC-derived PE-EVT have reduced surface HLA-G, blunted invasive capacity, and altered EVT-specific gene expression. These abnormalities correlated with promoter hypermethylation of genes associated with the epithelial-mesenchymal transition pathway, specifically in primed-iPSC derived PE-EVT. Our findings indicate that abnormal epigenetic regulation might play a role in PE pathogenesis.

Digital microfluidic biochips (DMFBs) have a significant stride in the applications of medicine and the biochemistry in recent years. DMFBs based on micro-electrode-dot-array (MEDA) architecture, as the next-generation DMFBs, aim to overcome drawbacks of conventional DMFBs, such as droplet size restriction, low accuracy, and poor sensing ability. Since the potential market value of MEDA biochips is vast, it is of paramount importance to explore approaches to protect the intellectual property (IP) of MEDA biochips during the development process. In this paper, an IP authentication strategy based on the multi-PUF applied to MEDA biochips is presented, called bioMPUF, consisting of Delay PUF, Split PUF and Countermeasure. The bioMPUF strategy is designed to enhance the non-linearity between challenges and responses of PUFs, making the challenge–response pairs (CRPs) on the MEDA biochips are difficult to be anticipated, thus thwarting IP piracy attacks. Moreover, based on the easy degradation of MEDA biochip electrodes, a countermeasure is proposed to destroy the availability of piracy chips. Experimental results demonstrate the feasibility of the proposed bioMPUF strategy against the brute force attack and modeling attack.

Background Resolvin D2 (RvD2) has been reported to protect against the development of atherosclerosis in animal models. The objective of this study was to examine the prospective association between plasma RvD2 and the risk of atherosclerotic cardiovascular disease (ASCVD) at the population level. Methods and Results A cohort of 2633 community‐dwelling individuals aged 35–60 years was followed for 8 years in this study. Adjusted hazard ratios and 95% CIs for ASCVD outcomes according to baseline RvD2 levels were calculated using Cox proportional hazards models. Mediation analysis was used to test the indirect effect of serum cholesterol indicators on the association between RvD2 and ASCVD probability. In total, 284 new cases of ASCVD were identified during follow‐up. An inverted U‐shaped association between natural log (ln)‐transformed RvD2 and incident ASCVD was determined, and the threshold value for lnRvD2 was 3.87. Below the threshold, each unit increase in lnRvD2 was associated with a 2.05‐fold increased risk of ASCVD (95% CI, 1.13–3.74; P=0.019). Above the threshold, each unit increase in lnRvD2 was associated with a 36% reduced risk of ASCVD (95% CI, 0.51–0.80; P

Long-range multi-purpose cluster munitions with reconnaissance, identification, strike and evaluation functions are widely used, and a large number of unexploded submunitions will be generated on the surface, which will greatly hinder the battlefield maneuver, deployment and depth attack. Due to its special visual characteristics, surface unexploded submunitions use imaging technology and deep learning technology for long-distance, large-area, non-contact rapid and accurate detection and recognition. This paper introduces the common methods of unexploded submunition detection and their advantages and disadvantages, summarizes the imaging characteristics of unexploded submunitions under different imaging technology conditions and their identification and positioning methods, analyzes the signifi-cant advantages of deep learning in the field of unexploded submunition detection and recognition. Then, a UAV-borne rapid detection, identification and positioning method based on deep learning for surface unexploded submunitions is proposed.

Abstract Aim Rates vary widely across hospitals globally and typically range from 3 to 11 falls per 1000 bed days and as 7–11 in Affiliated Hospital of Nantong University. This study determined to explore contributing factors and poor prognosis of fall in elderly tumour patients in China. Design A cross‐sectional study. Methods 161 older adults were invited to participate in this study and completed a self‐reported questionnaire, took blood tests, and received the exam of musculoskeletal ultrasound. Results Among 161 patients, falls occurred in 41 cases, accounting for 24.8%. 51.6% of older adults suffered from intermediate‐to‐high risk of falls. Fall history, reduced self‐care ability, sleep disturbance, hearing impairment, hyperkyphosis, chronic disease, platelet count, and the thickness of left muscle rectus femoris (LF‐MLT), and left cross‐sectional area (LF‐CSA) were all contributing factors of fall, and higher risk of fall indicating lower quality of life. A fall prediction model was established in this study based on above contributing factors with good prediction efficiency (AUC = 0.920). Patient or public contribution The patient volunteers participated in this study and provided valuable data for the final analysis and the acquisition of conclusion.

【Objective】This study aims to discover members of the Al-activated malate transporter ALMT gene family that may be involved in the growth and development of litchi and to investigate their biological functions.【Method】The physicochemical properties of proteins, subcellular localisation predictions, phylogeny, gene structure, conservative motifs, chromosomal positioning, promoter cis-acting elements, protein structure and expression patterns of litchi ALMT gene family members were systematically analysed using bioinformatics methods, expression of the litchi LcALMTs was analysed by qPCR.【Result】The results showed that the litchi LcALMT gene family has 16 members with CDS lengths ranging from 118 to 803 bp and isoelectric points between 5.16 and 9.07. Subcellular localisation predictions showed that the LcALMTs were all localised to the plasma membrane, and the family was divided into five subclades based on a phylogenetic tree. The number of exons of the LcALMTs ranged from 3 to 10, and four LcALMT genes did not contain any non-coding regions. Chromosomal localisation analysis revealed that LcALMTs was localised on only six of the 15 chromosomes of litchi, and mainly on chromosome 13. Conservative motif analysis revealed that litchi ALMT family members contain both ALMT and ALMT superfamily structural domains. Among the cis-acting elements, light-responsive elements accounted for the largest proportion, followed by hormone-responsive elements. Expression pattern analysis revealed that the expression of Litchi LcALMTs was highly variable in different tissues, with LcALMT5 and LcALMT15 being expressed at high levels in all tissues, the qPCR results showed that the expression level of LcALMT5 was more consistent with the results of the transcriptome.【Conclusion】There are 16 members of the litchi ALMT Gene family, which has a conservative gene structure and protein domain, and there are differences in tissue expression.

Abstract During the COVID-19 lockdown in Shanghai, we found many social exchanges within communities. In this study, we focus on exploring the driving forces behind social exchanges and their impacts on the social solidarity of local communities. Twenty-eight residents of separate communities are interviewed, and grounded theory is applied to have the motivation for social exchanges coded and analyzed. We find that reciprocal and generalized exchanges, rather than negotiated exchanges, occur in most communities. Among these exchanges, the willingness to share is stimulated by the traditional cultural norms taking place in online social groups. Residents will be propelled to carry on exchanges through sentiments cultivated within and to develop the micro social order through interactions in the media space. Social exchanges bring social solidarity to communities and develop it into a noticeable form consisting of functional, emotional, and communal aspects.

Metabolomics, especially urine-based studies, offers incredible promise for the discovery and development of clinically impactful biomarkers. However, due to the unique challenges of urine, a highly precise and reproducible workflow for NMR-based urine metabolomics is lacking. Using 1D and 2D non-uniform sampled (NUS) 1H-13C NMR spectroscopy, we systematically explored how changes in hydration or specific gravity (SG) and pH can impact biomarker discovery. Further, we examined additional sources of error in metabolomics studies and identified Navigator molecules that could monitor for those biases. Adjustment of SG to 1.002–1.02 coupled with a dynamic sum-based peak thresholding eliminates false positives associated with urine hydration and reduces variation in chemical shift. We identified Navigator molecules that can effectively monitor for inconsistencies in sample processing, SG, protein contamination, and pH. The workflow described provides quality assurance and quality control tools to generate high-quality urine metabolomics data, which is the first step in biomarker discovery.

Due to technical barriers and economic costs, malicious circuits, known as hardware Trojans, are easily implanted in the complicated integrated circuit design and manufacturing process, which can lead to many disastrous consequences, such as denial of service, information leakage, performance degradation, etc. Research on how to detecting hardware Trojans has grown into a significantly open issue over the past decade. While, for very large scale integrated circuits, numerous new challenges deserve our full attention, including golden-free chip reference, automatic feature engineering, hardware Trojan localization, and scalable framework. In response to the above challenges, a fine-grained gate-level hardware Trojan detection approach is proposed in this paper, named GateDet, from improving earlier circuit graph modeling to developing a detection framework based on Bidirectional Graph Convolution Networks with a timely information fusion strategy. GateDet achieves automatic feature circuit extraction and further overcomes the original neighborhood limitation of Bidirectional Graph Convolution Network. Moreover, for large-scale training, it comprehensively considers the problems of sample imbalance and boundary network, and develops a circuit directed graph sampling method based on GraphSAINT, which improves the training performance of the directed graph framework. From experiments, GateDet shows high scalability on 24 benchmarks of TrustHub. It could be used to learn about adaptive structural feature extraction for different Trojans simultaneously. Compared to the existing gate-level detections, the fine-grained results of GateDet are more accurate and can be used to track suspicious structures, reducing manual review.

This study presents a novel approach for the reuse of uncontaminated fracturing flowback fluids to improve the inhibitory and lubricating properties of water-based drilling fluids (WBFs), curb environmental pollution arising from flowback fluids, and substantially mitigate the expenses associated with WBFs. The experimental design was optimized using orthogonal experiments and range analyses, whereby the modified rubber powder was set at 2.0%, xanthan gum at 0.15%, and a plant phenol to modified complexing agent ratio of 1:0.01. The assessment of the performance evaluation tests indicated that the use of uncontaminated fracturing flowback fluids as the base water can remarkably enhance the inhibitory and lubricating properties of WBFs. Precisely, this approach reduces the linear expansion rate from 62.31% to 21.25%, the reduction rate of extreme pressure lubrication coefficient by 87.98%, and the reduction rate of mud cake sticking factor by 59.86%. This investigation has established the potential environmental and economic benefits of reusing clean fracturing flowback fluids in WBFs.

Abstract In this work, the $$\gamma p\rightarrow \phi (1680) p$$ γ p → ϕ ( 1680 ) p reaction is systematically studied based on the two gluon exchange model and the pomeron model. The calculation results show that the total cross section of $$\phi (1680)$$ ϕ ( 1680 ) photoproduction can reach more than 80 nb near the center-of-mass energy $$W=4$$ W = 4 GeV, so it is suggested that JLab and other experiments carry out relevant measurements. Moreover, under the framework of the vector meson dominance assumption, the scattering length of $$\phi (1680)$$ ϕ ( 1680 ) and proton interaction is estimated as $$0.07\pm 0.01$$ 0.07 ± 0.01 fm from the cross sections predicted by the theoretical models, which is on the same order of magnitude as the $$\phi (1020)$$ ϕ ( 1020 ) -p scattering length. In addition, the total cross section, number of events and rapidity distribution of $$\phi (1680)$$ ϕ ( 1680 ) production are predicted at Electron-Ion colliders (EICs) and ultra-peripheral collisions (UPCs) by using eSTARlight and STARlight code. The study of $$\phi (1680)$$ ϕ ( 1680 ) and relevant properties is of enlightening significance in better understanding the strangeonium family spectrum, and provides necessary data support for the future experiment of EICs and UPCs.

Carbon electrode perovskite solar cell has great potential in commercial application based on its low cost, superior stability, and facile fabrication process. However, its performance still lags behind that of devices with gold anode, which greatly attributes to the insufficient charge transport and collection at carbon anode side. Herein, efficient anode buffer contact is built by constructing an organic/inorganic composite hole transport bilayer together with strategic carbon precovering/postcovering anode. Adding a charge selective polythiophene (P3HT) layer between perovskite and nickel oxide (NiOx) can reduce charge transfer recombination loss, inhibit moisture infiltration, and crystallographically protect the perovskite underneath from being corroded by carbon paste. Together with a dense and pin-hole free hole transport layer (HTL)/carbon contact through precovering diluted carbon paste, the modified anode buffer interfaces also promote a facilitated charge collection, thus enhancing the photovoltaic performance of low-cost carbon electrode perovskite solar cells to a state-of-the-art value of 20.8% with an enhanced operational stability. This work provides adaptable and robust anode buffer interfaces for efficient, stable and low cost carbon electrode perovskite cells toward commercialization.

Abstract With the high penetration of distributed generation and gradual increase in loop network structures, existing over‐current protection in distribution networks is difficult to apply. The use of current differential protection has become an ideal solution, and high performance and commercialization of 5G communication provides an effective way to solve the problem of differential protection channel transmission. Aiming at distribution network differential protection based on 5G, this paper adopted an adaptive frequency conversion transmission mechanism to significantly reduce the data flow of differential protection. In terms of protection strategy, the differential allowable signal and the differential protection startup logic are separated, and a novel action method and protection logic suitable for weak infeed distribution networks are proposed. The strategy significantly shortens the protection action time under wireless communication. Furthermore, a comprehensive synchronization scheme using zero‐crossing synchronization of forward sampling points and cycle numbers based on the second pulse signal was established. This scheme can effectively improve the reliability of data synchronization for differential protection. Finally, the method proposed in this study was verified using the dynamic model experiment of real‐time digital simulation (RTDS).

The Internet of Vehicles (IoV) has a significant impact on improving traffic efficiency and driving safety. In this paper, we propose an intelligent transportation credit system based on blockchain, and design a crossroad passing smart contract that allows time-sensitive convoys to pass a crossroad earlier by paying traffic tokens for right-of-way trade. Second, this paper formulates the time–cost optimization problem under the premise of protecting the privacy of preceding convoys. Based on game theory with incomplete information, two right-of-way optimal bid strategies are given. Both theoretical analysis and simulation prove that the strategy proposed in this paper effectively reduces the waiting time of time-sensitive vehicles at a crossroad and increases the trade success probability, which, in turn, improves the expected total profit of convoys and achieves Pareto improvement.

Green synthesis of metal-organic frameworks (MOFs) in water with alleviated environmental influence and reduced cost is an essential step to transfer laboratory MOFs research to industrial application. Switching from the commonly used organic solvents to pure water encounters challenges of the poor solubility of organic linkers, slow reaction kinetics, and the formation of polymorphic products. So far, a universal MOFs synthetic strategy in water system has yet to be developed. This study reports the seed-aided synthesis of eleven MOFs with diverse compositions and structures while pure water serving as solvent. The corresponding reaction temperature and time of using this new strategy were reduced compared with original synthetic approaches, while the products maintain porous structure and high crystallinity. The success of this strategy relies on the addition of parent MOFs as seeds which could promote crystallization process by skipping the time-consuming induction period and avoiding the formation of polymorphic impurities.

Abstract In the quantum chromodynamics, the mass source of the proton is decomposed into four parts by the energy momentum tensor: quark energy term, gluon energy term, quark mass term and trace anomaly term. And the trace anomaly term is the most crucial contribution for studying the internal structure of the proton. In this work, under the definition of the vector meson dominant model, the trace anomaly contribution of the proton is extracted from the experimental data of light vector mesons $$\rho $$ ρ , $$\omega $$ ω and $$\phi $$ ϕ photoproduction at the near-threshold, which are ( $$0.53\pm 0.15$$ 0.53 ± 0.15 ) $$\%$$ % , ( $$1.47\pm 0.48$$ 1.47 ± 0.48 ) $$\%$$ % and ( $$2.73\pm 0.54$$ 2.73 ± 0.54 ) $$\%$$ % , respectively. Further, combined with the results extracted from the heavy vector meson $$J/\psi $$ J / ψ photoproduction, the distribution of the trace anomaly contribution with the vector meson mass is given for the first time. These results may provide important theoretical information for determining the range of the trace anomaly contribution and for further understanding the origin of the proton mass.

The primary feature of Industry 4.0 is MPP (mass personalization production), which requires that consumers’ individual requests are met in large-scale production. Under MPP, there is a multitude of subtasks decomposed from production tasks that are derived from individualized requests, and allocating these subtasks properly brings high economic benefits. However, existing approaches to achieve MPP, such as cloud manufacturing and social manufacturing, generally can not provide customers with deep participation in the entire production cycle, or respond to consumers’ modification needs by a triggered mechanism. Besides, some methods are of centralized architecture, which is vulnerable to single point error and with large cloud load that is not conducive to quickly responding to consumers’ dynamic demand changes. Therefore, this paper proposes a dynamic edge-cloud manufacturing mode for MPP, which can make subtask allocation with high economic benefit through distributed computing and implementing modifications of alternating direction method of multiplier (ADMM) algorithm. Also, it proposes an original improved ADMM algorithm, named Relaxation-Based ADMM algorithm, to increase the optimization speed in large-scale cases. The experimental results show that the proposed method generally obtains a superior solution under a certain iteration count.

ABSTRACT In the situation of mass vaccination against COVID-19, few studies have reported on the early kinetics of specific antibodies (IgG/IgM/IgA) of vaccine breakthrough cases. There is still a lack of epidemiological evidence about the value of serological indicators in the auxiliary diagnosis of COVID-19 infection, especially when the nucleic acid results were undetectable. Omicron breakthrough cases post-inactivated vaccination (n = 456) and COVID-19-naive individuals with two doses of inactivated vaccination (n = 693) were enrolled. Blood samples were collected and tested for SARS-CoV-2 antibody levels based on the magnetic chemiluminescence enzyme immunoassay. Among Omicron breakthrough cases, the serum IgG antibody level was 36.34 Sample/CutOff (S/CO) (95% confidence interval [CI], 31.89 to 40.79) in the acute phase and 88.45 S/CO (95% CI, 82.79 to 94.12) in the recovery phase. Serum IgA can be detected in the first week post-symptom onset (PSO) and showed an almost linear increase within 5 weeks PSO. Compared with those of breakthrough cases, IgG and IgA titers of the postimmune group were much lower (4.70 S/CO and 0.46 S/CO, respectively). Multivariate regression showed that serum IgG and IgA levels in Omicron breakthrough cases were mainly affected by the weeks PSO (P

Digital microfluidic, as an emerging and potential technology, diversifies the biochemical applications platform, such as protein dilution sewage detection. At present, a vast majority of universal cyberphysical digital microfluidic biochips (DMFBs) transmit data through wires via personal computers and microcontrollers (like Arduino), consequently, susceptible to various security threats and with the popularity of wireless devices, losing competitiveness gradually. On the premise that security be ensured first and foremost, calls for wireless portable, safe, and economical DMFBs are imperative to expand their application fields, engage more users, and cater to the trend of future wireless communication. To this end, a new cyber-physical DMFB called PortableLab is proposed in this paper, which guarantees data security through wireless sensors at low cost. After considering the security, computing consumption, and cost, a mobile module is added. In addition, the improved Advanced Encryption Standard (AES) and Cyclic Redundancy Check (CRC) algorithms are utilized to ensure the integrity and confidentiality of data transmission. Ultimately, all the security analysis, cost analysis, and experimental results on multiple protocols demonstrate the feasibility of the proposed PortableLab DMFB in time and space.

In this research, we present a cooperative communication network based on two energy-harvesting (EH) decode-and-forward (DF) relays that contain the harvest-store-use (HSU) architecture and may harvest energy from the surrounding environment by utilizing energy buffers. To improve the performance of this network, an opportunistic routing (OR) algorithm is presented that takes into account channel status information, relay position, and energy buffer status, as well as using the maximal ratio combining (MRC) at the destination to combine the signals received from the source and relays. The theoretical expressions for limiting distribution of energy stored in infinite-size buffers are derived from the discrete-time continuous-state space Markov chain model (DCSMC). In addition, the theoretical expressions for outage probability, throughput, and per-packet time slot cost in the network are obtained utilizing both the limiting distributions of energy buffers and the probabilities of transmitter candidates set. Through numerous simulation results, it is demonstrated that simulation results match with corresponding theoretical results.

As modern vehicles become more intelligent and connected, the number of ECUs and communication interfaces with external networks (such as 3G/4G and Bluetooth) has increased significantly, which raises potential network security risks. Due to the absence of effective security measures, there is a frequent occurrence of cybersecurity incidents targeting vehicles, particularly the in-vehicle CAN bus network. As the main bus in the vehicle, the CAN bus faces important challenges in its safety due to the lack of security mechanisms. Therefore, we propose a CAN bus intrusion detection system based on multiple observation HMM for in-vehicle networks to enhance the security of the vehicle. Specifically, the proposed algorithm builds a multiple observation HMM-based on the ID and data fields of normal CAN bus traffic. According to the established HMMs, we calculate the existence probability of the frame under the defined time window as the detection threshold. When the existence probability of the frame to be detected exceeds the normal threshold range, it is considered abnormal. Furthermore, we establish four common attack models based on the collected real vehicle data and evaluate the performance of the proposed algorithm in these attack scenarios. The experimental results show that the proposed method has better detection performance than other frame-by-frame anomaly detection methods in four attack scenarios.

Traditional liquid-solid extraction methods for the removal of zinc ions (Zn(II)) were generally limited by the leakage of extractant and low extraction capacity. The two-component Pickering emulsion hydrogels (PEHGs) in which the synergistic 2-ethylhexyl hydrogen-2-ethylhexylphosphate/tributyl phosphate (EHEHPA/TBP) was encapsulated in a semi-penetrating polymer network (SIPN) structured hydrogels polyacrylamide/sodium alginate (PAM/SA) were prepared by the Pickering emulsions polymerization route. The PEHGs were characterized by FTIR, SEM, TGA, and tensile mechanical measurements, and their self-healing properties were explored. The adsorption performance of PEHGs on Zn(II) was investigated. The results showed that the encapsulation of the extractant reached 95% due to the hydrogels network and nano-silica (nano-SiO2) particle network in PEHGs-15. The critical crashing pressure of PEHGs-15 was 0.084 MPa, and the adsorption after 3 h of healing was only 86% of the original amount. The maximum adsorption capacity of PEHGs-15 on Zn(II) was 76.51 mg/g at pH 5. The functional groups of SA and EHEHPA/TBP enhanced the adsorption capacity of PEHGs-15 by chelating with Zn(II). After five adsorption-desorption cycles, the adsorption capacity of PEHGs-15 exceeded 85% of the initial one. The excellent mechanical properties, self-healing, and regenerative properties of PEHGs-15 offer the potential to remove Zn(II) from the solution. HIGHLIGHTS The two-component PEHGs based on Pickering emulsions were prepared to remove Zn(II) from the solution.; The introduction of SA and nano-SiO2 resulted in 95% encapsulation of extractants in PEHGs.; The synergistic extractant EHEHPA/TBP and PAM/SA in PEHGs-15 exhibited a high adsorption capacity of 76.51 mg/g for Zn(II).; After five adsorption-desorption cycles, the adsorption capacity of PEHGs-15 exceeded 85% of the initial one.;

Abstract Background Emphasizes the state of academic stress, social support, and self-regulatory fatigue on the physical and mental development of Chinese nursing students, the purpose of this study was to investigate the relationship between these variables and the mediating role of social support in academic stress and self-regulatory fatigue among a group of undergraduate nursing students in Heilongjiang Province, China, in order to provide a theoretical basis for working to reduce nursing students’ self-regulatory fatigue. Methods In this cross-sectional study, 1703 nursing students from various academic years completed the scales of social support, academic stress, and self-regulatory fatigue. In the end, there were 797 valid questionnaires, for a recovery rate of 46.80%. For statistical analysis, the independent t-test, Kruskal Wallis test, and Pearson correlation coefficient were used. In addition, we undertake analyses using structural equation modeling. Results The bulk of nursing students, or 81.4%, are between the ages of 19 and 21. Eighty percent were females. The bulk (93.0%) was comprised of freshmen. Academic stress, social support, and self-regulatory fatigue had total scores of 111.28 ± 29.38, 37.87 ± 6.70, and 45.53 ± 5.55,respectively. Academic stress was correlated with social support and self-regulatory fatigue (all p

In this work, a dynamic structured light 3D reconstruction method adapted to random phase shift step size is proposed for the problem of dynamic workpiece 3D reconstruction in industrial manufacturing scenes. The method firstly utilizes a structured light sinusoidal grating pattern to match the relative phase shift of the workpieces moving along the perpendicular direction of the stripes on the conveyor belt. Secondly, in order to solve the problem of non-uniform phase shift step length due to external vibration, electromagnetic radiation and other disturbing factors of the conveyor belt and other mechanical devices, the method proposes a dynamic structured light 3D reconstruction model based on RPSNet, which is based on the CycleGAN network model, and uses the AIR2U-net model proposed in this paper as the generator, and the multilayer convolutional neural network CNN as the discriminator to realize the grating map. discriminator to realize the conversion of raster map to depth map. Finally, for the problem that the network model needs a large amount of data for training and it is difficult to collect data in the actual industrial scene, this paper uses the Thing10k dataset, and the dataset made by Blender simulation software for model training. Finally, a higher quality 3D reconstruction of the workpiece is realized.

Electronic tickets (e-tickets) are gradually being adopted as a substitute for paper-based tickets to bring convenience to customers, corporations, and governments. However, their adoption faces a number of practical challenges, such as flexibility, privacy, secure storage, and inability to deploy on IoT devices such as smartphones. These concerns motivate the current research on e-ticket systems, which seeks to ensure the unforgeability and authenticity of e-tickets while simultaneously protecting user privacy. Many existing schemes cannot fully satisfy all these requirements. To improve on the current state-of-the-art solutions, this paper constructs a blockchain-enhanced privacy-preserving e-ticket system for IoT devices, dubbed PriTKT, which is based on blockchain, structure-preserving signatures (SPS), unlinkable redactable signatures (URS), and zero-knowledge proofs (ZKP). It supports flexible policy-based ticket purchasing and ensures user unlinkability. According to the data minimization and revealing principle of GDPR, PriTKT empowers users to selectively disclose subsets of (necessary) attributes to sellers as long as the disclosed attributes satisfy ticket purchasing policies. In addition, benefiting from the decentralization and immutability of blockchain, effective detection and efficient tracing of double spending of e-tickets are supported in PriTKT. Considering the impracticality of existing e-tickets schemes with burdensome ZKPs, we replace them with URS/SPS or efficient ZKP to significantly improve the efficiency of ticket issuing and make it suitable for use on smartphones.

BackgroundAlthough cellular and animal studies have reported that resolvin D1 (RvD1) and resolvin D2 (RvD2) are mechanisms involved in the development of type 2 diabetes mellitus (T2DM), the impact of RvD1 and RvD2 on the risk of T2DM at a population level remains unclear.MethodsWe included 2755 non-diabetic adults from a community-based cohort in China and followed them for seven years. Cox proportional hazards model was used to estimate hazard ratios (HRs) and 95% confidence intervals (CIs) for the association of RvD1 and RvD2 with T2DM probability. Time-dependent receiver operator characteristics (ROC) curve was used to evaluate the predictive performance of RvD1 and RvD2 for the risk of T2DM based on the Chinese CDC T2DM prediction model (CDRS).ResultsA total of 172 incident T2DM cases were identified. Multivariate-adjusted HRs (95% CI) for T2DM across quartiles of RvD1 levels (Q1, Q2, Q3 and Q4) were 1.00, 1.64 (1.03-2.63), 1.80 (1.13-2.86) and 1.61 (1.01-2.57), respectively. Additionally, body mass index (BMI) showed a significant effect modification in the association of RvD1 with incident T2DM (Pinteraction = 0.026). After multivariate adjustment, the HR (95% CI) for T2DM in the fourth compared with the first quartile of RvD2 was 1.94 (95% CI: 1.24-3.03). Time-dependent ROC analysis showed that the area under time-dependent ROC curves of the “CDRS+RvD1+RvD2” model for the 3-, 5- and 7-year risk of T2DM were 0.842, 0.835 and 0.828, respectively.ConclusionsHigher RvD1 and RvD2 levels are associated with a higher risk of T2DM at the population level.

Hypergraph, as a generalization of the notions of graph and simplicial complex, has gained a lot of attention in many fields. It is a relatively new mathematical model to describe the high-dimensional structure and geometric shapes of data sets. In this paper,we introduce the neighborhood hypergraph model for graphs and combine the neighborhood hypergraph model with the persistent (embedded) homology of hypergraphs. Given a graph,we can obtain a neighborhood complex introduced by L. Lovász and a filtration of hypergraphs parameterized by aweight function on the power set of the vertex set of the graph. Theweight function can be obtained by the construction fromthe geometric structure of graphs or theweights on the vertices of the graph. We show the persistent theory of such filtrations of hypergraphs. One typical application of the persistent neighborhood hypergraph is to distinguish the planar square structure of cisplatin and transplatin. Another application of persistent neighborhood hypergraph is to describe the structure of small fullerenes such as C20. The bond length and the number of adjacent carbon atoms of a carbon atom can be derived from the persistence diagram. Moreover, our method gives a highly matched stability prediction (with a correlation coefficient 0.9976) of small fullerene molecules.

Using Tianbao banana as the test material, one strain of Fusarium was isolated, purified and identified. Different concentrations (5, 10, 15, 20 mg/mL and 25 mg/mL) of camellia saponin were used to determine the inhibitory effect on Fusarium. The weight loss rate, hardness, soluble solids, titratable acid, respiratory strength and other main quality indicators of bananas treated with different concentrations of camellia saponin (15, 20, 25 mg/mL and 30 mg/mL) at a temperature of 16 ℃ and a relative humidity of 85% under constant temperature and humidity for 25 days were determined to study the effect of camellia saponin on bananas. The results showed that the antibacterial rate of camellia saponin against Fusarium continued to increase as the concentration increased. When the concentration of camellia saponin was 20 mg/mL, the antibacterial rate could reach 86.52%, but when the concentration increased again, the antibacterial rate did not increase significantly. On this basis, the MIC and MBC values of camellia saponin against Fusarium were determined to be 5 mg/mL and 10 mg/mL, respectively. Comprehensive quality indicators showed that the 20 mg/mL concentration of camellia saponin solution had the best preservation effect on post-harvest bananas. When stored for 20 days, compared with the control group (clear water group and positive control group), the weight loss rate was reduced by 48.04% and 51.67%, respectively. Compared with the control group (clear water group), soluble solids delayed the emergence of the peak value. Titratable acid increased by 56.09% and 23.07%, and respiratory intensity increased by 65.68% and 14.82%. On the 10th day of storage, the hardness increased by 13.76% compared with the positive control group. The results of the research are expected to provide certain theoretical and technical support for the preservation of bananas by camellia saponin.

Warm-season O3 pollution has been increasingly frequent worldwide in the past few years, exposing a threat to human health as well as the natural environment. Here, the authors showcase a stable MOF which can not only effectively capture various airborne VOCs, but decompose trace O3 in ambient air.

Objective To explore the feasibility of establishing a computer-aided diagnostic model of multi-stained pathological images in patients with non-inflammatory aortic medial degeneration(MD). Methods In this study, pathological sections of aortic surgical specimens for non-inflammatory lesions from patients with thoracic aortic aneurysms and dissections were retrospectively collected at the Beijing Anzhen Hospital, Capital Medical University from July to December 2018. The lesions were scanned under ×400 magnification as whole slide images(WSI) and then annotated by two pathologists. The annotated WSI images were randomly split into training and test sets in a 6:1 ratio. SE-EmbraceNet was used to train the data to construct a multi-classification model for MD of multi-stained pathology images, including intralamellar mucoid extracellular matrix accumulation (MEMA-I), translamellar mucoid extracellular matrix accumulation (MEMA-T), elastic fiber fragmentation and/or loss(EFFL) and smooth muscle cell nuclei loss (SMCNL). The classification effect of the model was evaluated based on the test set data, and the results were expressed in terms of accuracy, sensitivity, precision, and the F1 value. Results Totally 530 pathological slides of non-inflammatory aortic lesion surgical specimens from patients with aortic aneurysm and dissection were included. Extracted 5265 sets of images, each containing 5 stained pathological images of the same lesion site: HE staining, special staining (elastic fiber/VanGieson, Masson, Alcian blue/periodic acid Schiff) and smooth muscle actin staining. There were 4513 sets of training images, including 987 SMCNL, 2013 EFFL, 1337 MEMA-I, and 176 MEMA-T; and 752 test images including 166 SMCNL, 335 EFFL, 222 MEMA-I, and 29 MEMA-T. The overall performance of the model in the test set showed good results, with an accuracy of 96.54%(726/752). The model had the best classification performance for EFFL, with accuracy, sensitivity, precision, and F1 value all ≥98.51%. The model also had a great classification ability for SMCNL, with all evaluated indexes≥97.59%. Conclusion The multi-stained pathology image-based MD classification model constructed in this study has high classification accuracy and good generalization ability, which has the potential to be applied to assist in the diagnosis of the non-inflammatory aortic lesion.

Here the authors perform a genome-wide CRISPR-Cas9 knockout screen to systematically identify and characterize essential and growth-restricting genes in human trophoblast cells. They identify TEAD1 as a key regulator that plays an important role in the specification, maintenance, and differentiation of the human trophoblast lineage by modulating chromatin architecture and gene expression.

Abstract Background Lycopene epsilon-cyclase (ε-LCY) is a key enzyme in the carotenoid biosynthetic pathway (CBP) of higher plants. In previous work, we cloned two Ntε-LCY genes from allotetraploid tobacco (Nicotiana tabacum), Ntε-LCY2 and Ntε-LCY1, and demonstrated the overall effect of Ntε-LCY genes on carotenoid biosynthesis and stress resistance. However, their genetic and functional characteristics require further research in polyploid plants. Results Here, we used CRISPR/Cas9 to obtain Ntε-LCY2 and Ntε-LCY1 mutants in allotetraploid N.tabacum K326. Ntε-LCY2 and Ntε-LCY1 had similar promoter cis-acting elements, including light-responsive elements. The Ntε-LCY genes were expressed in roots, stems, leaves, flowers, and young fruit, and their highest expression levels were found in leaves. Ntε-LCY2 and Ntε-LCY1 genes responded differently to normal light and high light stress. Both the Ntε-LCY2 and the Ntε-LCY1 mutants had a more rapid leaf growth rate, especially ntε-lcy2-1. The expression levels of CBP genes were increased in the ntε-lcy mutants, and their total carotenoid content was higher. Under both normal light and high light stress, the ntε-lcy mutants had higher photosynthetic capacities and heat dissipation levels than the wild type, and this was especially true of ntε-lcy2-1. The reactive oxygen species content was lower in leaves of the ntε-lcy mutants. Conclusion In summary, the expression patterns and biological functions of the Ntε-LCY genes Ntε-LCY1 and Ntε-LCY2 differed in several respects. The mutation of Ntε-LCY2 was associated with a greater increase in the content of chlorophyll and various carotenoid components, and it enhanced the stress resistance of tobacco plants under high light.

Introduction: This paper proposes a sigmoid-based adaptive inertia control strategy for grid-forming (GFM) inverter to enhance frequency stability.Methods: Firstly, the frequency response characteristics under different disturbances are analyzed theoretically. Then, to solve the problem that fixed inertia leads to a contradiction between the maximum frequency deviation and the setting time, a non-linear inertia regulator is investigated. This non-linear inertia regulator, which is based on an improved sigmoid function, is applied to achieve real-time inertia by the frequency deviation. Moreover, the full-order small-signal model of the system containing the nonlinear inertia regulator is established and thus the stability of the proposed strategy is analyzed.Result and Discussion: The proposed control strategy is implemented without derivative action, which may suffer from high-frequency noises. Finally, the hardware-inthe-loop (HIL) results verify the remarkable performance of the proposed control strategy.

BackgroundAs an important early source of IL-17A and IL-22 in immune responses, type 3 innate lymphoid cells (ILC3s) are critically regulated by the transcription factor retinoic-acid-receptor-related orphan receptor gamma t (RORγt). Previously, we have identified a crucial role of the conserved non-coding sequence 9 (CNS9), located at +5,802 to +7,963 bp of the Rorc gene, in directing T helper 17 differentiation and related autoimmune disease. However, whether cis-acting elements regulate RORγt expression in ILC3s is unknown.ResultsHere we show that CNS9 deficiency in mice not only decreases ILC3 signature gene expression and increases ILC1-gene expression features in total ILC3s, but also leads to generation of a distinct CD4+NKp46+ ILC3 population, though the overall numbers and frequencies of RORγt+ ILC3s are not affected. Mechanistically, CNS9 deficiency selectively decreases RORγt expression in ILC3s, which thus alters ILC3 gene expression features and promotes cell-intrinsic generation of CD4+NKp46+ ILC3 subset.ConclusionOur study thus identifies CNS9 as an essential cis-regulatory element controlling the lineage stability and plasticity of ILC3s through modulating expression levels of RORγt protein.

Abstract The development of low‐cost, green, and pollution‐free counter electrode materials with high catalytic activity plays a critical role in improving the photovoltaic performance of dye‐sensitized solar cells (DSSCs). In recent years, transition metal phosphides have been widely used in DSSCs due to their outstanding catalytic activity and stability. Herein, a novel binary phosphide is immobilized on carbon paper (CP) by a two‐step strategy. This strategy involves the preparation of WO3 precursor by the hydrothermal method, and synthesis of tungsten phosphide (WP) with the vapor deposition method, which finally leads to the uniform dispersion of WP on carbon paper. The acquired WP/CP counter electrode demonstrates high electrical conductivity and prefect catalytic ability for reducing triiodide, and the DSSCs assembled with WP/CP counter electrode achieve a high‐power conversion efficiency of 10.29%, which is superior to that of the Pt‐based (7.34%). These findings illustrate that the WP microsheets in‐situ grown on carbon paper are a potential candidate to replace Pt as an economical and efficient counter electrode for DSSCs.

BackgroundMost studies have reported fecal microbiota transplantation (FMT) as an effective secondary option for Crohn’s disease (CD). However, there is little data on FMT as a first-line treatment for CD. In our study we explore the rates of clinical and endoscopic remission and mucosal healing after FMT plus partial enteral nutrition (PEN), as a first-line treatment for active CD in children.MethodsWe retrospectively enrolled pediatric CD patients who underwent PEN or PEN plus FMT treatment at diagnosis from November 2016 to July 2019 at the Pediatric Department, Tongji Hospital. The two groups were defined as FMT group (repeated and multiple doses of FMT plus PEN) or PEN group (PEN alone). All the patients received PEN intervention. At baseline and week 8- 10, the FMT group was administered multiple doses of FMT to help induce and maintain remission. All patients were evaluated at week 8- 10 and 18-22 via clinical and relevant laboratory parameters and endoscopic results. The clinical and endoscopic remission and mucosal healing rates were compared between the two groups at different time points after the therapy.ResultsTwenty-five newly diagnosed active CD patients were included in the study, containing 7 females and 18 males with a median age of 11. 1 ± 2.3 years. 13 and 12 patients were assigned to the PEN and FMT groups, respectively. At week 8-10, clinical remission was obtained in 83.3% and 53.8% of the FMT and PEN groups, respectively (p=0.202). The endoscopic remission rates were 72.7% for FMT and 25.0% for PEN (p=0.039), whereas the mucosal healing rates were 27.2% for FMT and 0% for PEN (p=0.093). At week 18-22, clinical remission was achieved in 72.7% and 20.0% of patients in the FMT and PEN groups, respectively (p=0.03). Theendoscopic remission rates were 66.6% and 12.5% in the FMT and PEN groups, respectively (p=0.05), whereas the mucosal healing rates were 55.5% and 0% in FMT and PEN groups, respectively (p=0.029).ConclusionThis study demonstrate that FMT plus PEN can be used as a first-line treatment for active CD in children.

Taking the Zhongqiu 1 Gas Field in the Tarim Basin as an example, the heterogeneity of large-scale mono-block gas fields and their primary controlling factors have been analyzed. Based on drilling core data, well log data, scanning electron microscopy, thin-section analysis, and mercury injection experiments, combining sedimentological interpretation, research on the reservoir characteristics and variability was carried out. The results showed that: (1) The lithologic characteristics showed obvious variations among wells in the Zhongqiu 1 gas field. Specifically, the main lithology developed in the Zhongqiu 1 well is feldspar lithic sandstone, while the remaining wells predominantly consist of lithic feldspar sandstone. These differences in rock composition maturity reveal that a higher proportion of stable mineral components leads to poorer reservoir properties; (2) the main factors controlling oil and gas productivity include the variations in petrology, mineralogy, and diagenetic process characteristics. The high content of unstable mineral components and constructive diagenesis could increase reservoir porosity together. (3) Sedimentary facies of the Bashijiqike Formation in the Zhongqiu 1 Gas Field played a dominant role in the reservoir distribution. The division of sedimentary facies zones reflects variations in material composition and grain size, serving as the main material basis for reservoirs. Differences in mineral composition reflect the sedimentary environment of the reservoir. Additionally, mineral composition indicates the relationship between diagenetic processes and reservoir evolution. The high feldspar content in well ZQ1 corresponded to relatively favorable reservoir properties. The dominant feldspar type was plagioclase, suggesting that early-stage chemical weathering had undergone significant alteration. The above conclusions provided a microscopic perspective to explain the differences in oil and gas production capacity of large delta-front gas fields, serving as a geological basis for the exploration and exploitation of similar fields.

【Objective】Acid invertase (SAI) plays an important role in litchi sugar metabolism. The litchi LcSAI promoter is cloned and analyzed in order to provide a theoretical basis for the research on the function of LcSAI in regulating litchi sugar metabolism.【Method】With the pulp of Feizixiao litchi as test material, according to the litchi genome sequence information, the 1 500 bp promoter sequence upstream of LcSAI gene was cloned. At the same time, the transcription start sites, cis-acting elements and CpG islands in the promoter sequences were predicted and analyzed by bioinformatics tools.【Result】A 1 514 bp LcSAI promoter sequence was cloned. In addition to a large number of promoter core elements such as TATA-box and enhancer element CAAT-box, promoter cis-acting elements also had light-responsive cis-elements, hormone response elements, anaerobic inducible response elements, and MYB binding sites, MYC binding sites and some cis-acting elements of unknown function. There may be three core promoter regions in the LcSAI promoter sequence. TheLcSAI promoter region did not predict CpG islands that met the defined conditions.【Conclusion】The LcSAI gene promoter is related to adversity stress. It is predicted that LcSAI plays an important role in the physiological process of litchi resisting adversity stress.

The study evaluates the outcomes of including varicella vaccines (VarV) in the local expanded programme on immunization (EPI) on the seropositivity rates and corresponding protective effects for children aged 3–6 years in Suzhou. The study is observational. Varicella prevalence in children was assessed based on data from the China Information System for Disease Control and Prevention (CISDCP) and the Jiangsu Province Vaccination Integrated Service Management Information System (JPVISMIS). Seropositivity was determined using the enzyme-linked immunosorbent assay (ELISA). A total of 2,873 children aged 3–6 years were enrolled in this study. The seropositivity rates were 95.31% and 86.89% for children with and without the strategy, respectively. The difference in seropositivity rate in children using the different strategies was statistically significant (Trend χ2 = 0.397, P = .255). It is therefore suggested that Suzhou had a high rate of occult infection before the inclusion of varicella vaccine in the EPI. The difference in seroprevalence rate between children with no history of varicella vaccination and those with a history of varicella vaccination was statistically different (χ2 = 51.362, P