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247 results on '"Chilton, N. B."'

4. Molecular detection and identification of spotted fever group rickettsiae in ticks collected from horses in Cuba

Author

Diaz-Sanchez, A. A., Chilton, N. B., Roblejo-Arias, L., Fonseca Rodriguez, Osvaldo, Marrero-Perera, R., Diyes, C. P., Yunik, M. E. M., Lobo-Rivero, E., Corona-Gonzalez, B., Diaz-Sanchez, A. A., Chilton, N. B., Roblejo-Arias, L., Fonseca Rodriguez, Osvaldo, Marrero-Perera, R., Diyes, C. P., Yunik, M. E. M., Lobo-Rivero, E., and Corona-Gonzalez, B.

Abstract

Spotted fever group (SFG) rickettsiae are obligatory intracellular bacteria that cause disease in humans and other animals. Ixodid ticks are the principal vectors of SFG rickettsiae. The present study aimed to determine the prevalence and species identity of SFG rickettsiae in ticks and horses from urban and rural areas of western Cuba using PCR assays. Tick samples, collected from 79 horses, consisted of 14 Amblyomma mixtum adults, 111 Dermacentor nitens adults and 19 pools of D. nitens nymphs (2-5 individuals/pool). The PCR results revealed the presence of Rickettsia spp. in 64% of the A. mixtum adults, 16% of the D. nitens adults, and 11% of the pooled samples of D. nitens nymphs. In contrast, Rickettsia spp. was not detected in any of the 200 horse blood samples included in this study. DNA sequence data of the rickettsial 17 kDa antigen gene showed that Rickettsia amblyommatis was present in A. mixtum; and Rickettsia felis in D. nitens. This is the first report of R. felis in D. nitens in Cuba. The present study extends our knowledge of the potential vector spectrum and distribution of SFG rickettsiae pathogens in western Cuba.

Published
2020
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20. Molecular detection and identification of spotted fever group rickettsiae in ticks collected from horses in Cuba.

Author

Díaz‐Sánchez, A. A., Chilton, N. B., Roblejo‐Arias, L., Fonseca‐Rodríguez, O., Marrero‐Perera, R., Diyes, C. P., Yunik, M. E. M., Lobo‐Rivero, E., and Corona‐González, B.

Subjects
*TICKS, *HORSES, *DERMACENTOR, *FEVER, *RICKETTSIA, *RHIPICEPHALUS, *CASTOR bean tick
Abstract

Spotted fever group (SFG) rickettsiae are obligatory intracellular bacteria that cause disease in humans and other animals. Ixodid ticks are the principal vectors of SFG rickettsiae. The present study aimed to determine the prevalence and species identity of SFG rickettsiae in ticks and horses from urban and rural areas of western Cuba using PCR assays. Tick samples, collected from 79 horses, consisted of 14 Amblyomma mixtum adults, 111 Dermacentor nitens adults and 19 pools of D. nitens nymphs (2–5 individuals/pool). The PCR results revealed the presence of Rickettsia spp. in 64% of the A. mixtum adults, 16% of the D. nitens adults, and 11% of the pooled samples of D. nitens nymphs. In contrast, Rickettsia spp. was not detected in any of the 200 horse blood samples included in this study. DNA sequence data of the rickettsial 17 kDa antigen gene showed that Rickettsia amblyommatis was present in A. mixtum; and Rickettsia felis in D. nitens. This is the first report of R. felis in D. nitens in Cuba. The present study extends our knowledge of the potential vector spectrum and distribution of SFG rickettsiae pathogens in western Cuba. [ABSTRACT FROM AUTHOR]

Published
2021
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23. Study of defects in silicon dioxide films on Si(100) by a variable-energy positron beam.

Author

Fujinami, M., Chilton, N. B., Ishii, K., and Ohki, Y.

Subjects
*AMORPHOUS semiconductors, *SILICA, *THIN films, *OXIDATION, *CHEMICAL vapor deposition
Abstract

Presents a study which investigated the defects of amorphous silicon dioxide films grown by thermal and plasma chemical vapor deposition in a tetraethylorthosilicate and oxygen atmosphere. DOppler broadening parameter in the silicon dioxide film; Experimentation on the oxidation of identical wafer; Results of the angular correlation of annihilation radiation.

Published
1993
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24. A slow positron beam study of vacancy formation in fluorine-implanted silicon.

Author

Fujinami, M. and Chilton, N. B.

Subjects
*POSITRON beams, *SILICON, *ION implantation, *SURFACE defects
Abstract

Discusses the results of a study on vacancy formation in fluorine-implanted silicon using a slow positron beam. Consequence of positron diffusion and the breadth of the positron implantation profile; Implications of the vacancies caused by ion implantation; Techniques used in demonstrating the correlation of defects present and vacancies in silicon.

Published
1993
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28. Speciation in the genus Cloacina (Nematoda: Strongylida): species flocks and intra-host speciation.

Author

CHILTON, N. B., SHUTTLEWORTH, M. A., HUBY-CHILTON, F., KOEHLER, A. V., JABBAR, A., GASSER, R. B., and BEVERIDGE, I.

Subjects
*STRONGYLIDA, *HOST-parasite relationships, *NUCLEAR DNA, *RIBOSOMAL DNA, *GENETIC speciation
Abstract

Sequences of the first and second internal transcribed spacers (ITS1 + ITS2) of nuclear ribosomal DNA were employed to determine whether the congeneric assemblages of species of the strongyloid nematode genus Cloacina, found in the forestomachs of individual species of kangaroos and wallabies (Marsupialia: Macropodidae), considered to represent species flocks, were monophyletic. Nematode assemblages examined in the black-striped wallaby, Macropus (Notamacropus) dorsalis, the wallaroos, Macropus (Osphranter) antilopinus/robustus, rock wallabies, Petrogale spp., the quokka, Setonix brachyurus, and the swamp wallaby, Wallabia bicolor, were not monophyletic and appeared to have arisen by host colonization. However, a number of instances of within-host speciation were detected, suggesting that a variety of methods of speciation have contributed to the evolution of the complex assemblages of species present in this genus. [ABSTRACT FROM PUBLISHER]

Published
2017
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