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124 results on '"Christophe Lagneau"'

1. Nonlinear SAR Modelling of Mosquito Repellents for Skin Application

Author

James Devillers, Adeline Larghi, Valérie Sartor, Marie-Laure Setier-Rio, Christophe Lagneau, and Hugo Devillers

Subjects
repellent, mosquitoes, Aedes aegypti, Structure–Activity Relationship (SAR), artificial neural network, Chemical technology, TP1-1185
Abstract

Finding new marketable mosquito repellents is a complex and time-consuming process that can be optimized via modelling. In this context, a SAR (Structure–Activity Relationship) model was designed from a set of 2171 molecules whose actual repellent activity against Aedes aegypti was available. Information-rich descriptors were used as input neurons of a three-layer perceptron (TLP) to compute the models. The most interesting classification model was a 20/6/2 TLP showing 94% and 89% accuracy on the training set and test set, respectively. A total of 57 other artificial neural network models based on the same architecture were also computed. This allowed us to consider all chemicals both as training and test set members in order to better interpret the results obtained with the selected model. Most of the wrong predictions were explainable. The 20/6/2 TLP model was then used for predicting the potential repellent activity of new molecules. Among them, two were successfully evaluated in vivo.

Published
2023
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2. Variation in the susceptibility of urban Aedes mosquitoes infected with a densovirus

Author

Aurélie Perrin, Anne-Sophie Gosselin-Grenet, Marie Rossignol, Carole Ginibre, Bethsabée Scheid, Christophe Lagneau, Fabrice Chandre, Thierry Baldet, Mylène Ogliastro, and Jérémy Bouyer

Subjects
Medicine, Science
Abstract

Abstract Urban Aedes mosquitoes are vectors of many viruses affecting human health such as dengue, chikungunya and Zika viruses. Insecticide resistance and environmental toxicity risks hamper the effectiveness of chemical control against these mosquito vectors. Alternative control methods, such as the use of mosquito-specific entomopathogenic viruses should be explored. Numerous studies have focused on evaluating the potential of different densoviruses species as biological control agents. However, knowledge on the extent of inter- and intra-specific variations in the susceptibility of Aedes mosquitoes to infection by different densoviruses remains insufficient. In this study, we compared infection and mortality rates induced by the Aedes albopictus densovirus 2 in different strains of Aedes albopictus and Aedes aegypti mosquitoes. The two Aedes species were different in terms of susceptibility to viral infection. Under laboratory conditions, Aedes albopictus densovirus 2 appeared more virulent for the different strains of Aedes aegypti tested than for those of Aedes albopictus. In addition, we also found significant intra-specific variation in infection and mortality rates. Thus, although even if Aedes albopictus densoviruses could be powerful biocontrol agents used in the management of urban Aedes populations, our results also call into question the use of single viral isolate as biocontrol agents.

Published
2020
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3. Efficacy of ULV and thermal aerosols of deltamethrin for control of Aedes albopictus in Nice, France

Author

Saïd C Boubidi, David Roiz, Marie Rossignol, Fabrice Chandre, Romain Benoit, Marc Raselli, Charles Tizon, Bernard Cadiou, Reda Tounsi, Christophe Lagneau, Didier Fontenille, and Paul Reiter

Subjects
Aedes albopictus, ULV, Cold fogging, Thermal fogging, Vector control, Dengue, Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background Ultra-low volume (ULV) insecticidal aerosols dispensed from vehicle-mounted cold-foggers are widely considered the method of choice for control of Aedes aegypti and Ae. albopictus during outbreaks of dengue and chikungunya and, more recently, Zika. Nevertheless, their effectiveness has been poorly studied, particularly in Europe. Nearly all published studies of ULV efficacy are bio-assays based on the mortality of caged mosquitoes. In our study we preferred to monitor the direct impact of treatments on the wild mosquito populations. This study was undertaken to evaluate the efficiency of the two widely used space spraying methods to control Ae. albopictus and Ae. aegypti. Methods We determined the susceptibility of local Ae. albopictus to deltamethrin by two methods: topical application and the “WHO Tube Test”. We used ovitraps baited with hay infusion and adult traps (B-G Sentinel) baited with a patented attractant to monitor the mosquitoes in four residential areas in Nice, southern France. The impact of deltamethrin applied from vehicle-mounted ULV fogging-machines was assessed by comparing trap results in treated vs untreated areas for 5 days before and 5 days after treatment. Four trials were conducted at the maximum permitted application rate (1 g.ha-1). We also made two small-scale tests of the impact of the same insecticide dispensed from a hand-held thermal fogger. Results Susceptibility to the insecticide was high but there was no discernable change in the oviposition rate or the catch of adult female mosquitoes, nor was there any change in the parous rate. In contrast, hand-held thermal foggers were highly effective, with more than 90% reduction of both laid eggs and females. Conclusions We believe that direct monitoring of the wild mosquito populations gives a realistic assessment of the impact of treatments and suggest that the lack of efficacy is due to lack of interaction between the target mosquitoes and the ULV aerosol. We discuss the factors that influence the effectiveness of both methods of spraying in the context of epidemic situations.

Published
2016
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4. DengueTools: innovative tools and strategies for the surveillance and control of dengue

Author

Annelies Wilder-Smith, Karl-Erik Renhorn, Hasitha Tissera, Sazaly Abu Bakar, Luke Alphey, Pattamaporn Kittayapong, Steve Lindsay, James Logan, Christoph Hatz, Paul Reiter, Joacim Rocklöv, Peter Byass, Valérie R. Louis, Yesim Tozan, Eduardo Massad, Antonio Tenorio, Christophe Lagneau, Grégory L'Ambert, David Brooks, Johannah Wegerdt, and Duane Gubler

Subjects
dengue, dengue control, dengue diagnostics, economic evaluation, entomology, climate change, early warning systems, risk mapping, surveillance, globalization, Public aspects of medicine, RA1-1270
Abstract

Dengue fever is a mosquito-borne viral disease estimated to cause about 230 million infections worldwide every year, of which 25,000 are fatal. Global incidence has risen rapidly in recent decades with some 3.6 billion people, over half of the world's population, now at risk, mainly in urban centres of the tropics and subtropics. Demographic and societal changes, in particular urbanization, globalization, and increased international travel, are major contributors to the rise in incidence and geographic expansion of dengue infections. Major research gaps continue to hamper the control of dengue. The European Commission launched a call under the 7th Framework Programme with the title of ‘Comprehensive control of Dengue fever under changing climatic conditions’. Fourteen partners from several countries in Europe, Asia, and South America formed a consortium named ‘DengueTools’ to respond to the call to achieve better diagnosis, surveillance, prevention, and predictive models and improve our understanding of the spread of dengue to previously uninfected regions (including Europe) in the context of globalization and climate change.The consortium comprises 12 work packages to address a set of research questions in three areas: Research area 1: Develop a comprehensive early warning and surveillance system that has predictive capability for epidemic dengue and benefits from novel tools for laboratory diagnosis and vector monitoring. Research area 2: Develop novel strategies to prevent dengue in children. Research area 3: Understand and predict the risk of global spread of dengue, in particular the risk of introduction and establishment in Europe, within the context of parameters of vectorial capacity, global mobility, and climate change.In this paper, we report on the rationale and specific study objectives of ‘DengueTools’. DengueTools is funded under the Health theme of the Seventh Framework Programme of the European Community, Grant Agreement Number: 282589 Dengue Tools.

Published
2012
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5. Mosquito densoviruses: the revival of a biological control agent against urban Aedes vectors of arboviruses

Author

Marie Rossignol, Jérémy Bouyer, Fabrice Chandre, Aurélie Perrin, Thierry Baldet, Bethsabée Scheid, Anne-Sophie Gosselin-Grenet, Mylène Ogliastro, Carole Ginibre, and Christophe Lagneau

Subjects
Aedes, 0303 health sciences, Aedes albopictus, biology, viruses, fungi, education, 030231 tropical medicine, Biological pest control, virus diseases, Aedes aegypti, medicine.disease, biology.organism_classification, medicine.disease_cause, Virology, 3. Good health, Dengue fever, 03 medical and health sciences, 0302 clinical medicine, medicine, Chikungunya, Densovirus, Aedes albopictus densovirus, 030304 developmental biology
Abstract

Urban Aedes mosquitoes are vectors of many viruses affecting human health such as Dengue, Chikungunya and Zika viruses. Insecticide resistance and environmental toxicity risks hamper the effectiveness of chemical control against these mosquito vectors. Alternative control methods, such as the use of mosquito-specific entomopathogenic viruses should be explored. Numerous studies have focused on evaluating the potential of different densoviruses species as biological control agent. However, knowledge on the extent of inter- and intra-specific variations in the susceptibility of Aedes mosquitoes to infection by different densoviruses remains insufficient. In this study, we compared infection and mortality rates induced by the Aedes albopictus densovirus 2 in different strains of Aedes albopictus and Aedes aegypti mosquitoes. The two Aedes species were different in terms of susceptibility to viral infection. Under laboratory conditions, Aedes albopictus densovirus 2 appeared more virulent for the different strains of Aedes aegypti tested than for those of Aedes albopictus. In addition, we also found significant intra-specific variation in infection and mortality rates. Thus, although even if Aedes albopictus densoviruses could be powerful biocontrol agents used in the management of urban Aedes populations, our results also call into question the use of single viral isolate as biocontrol agents.

Published
2020
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6. Practical management plan for invasive mosquito species in Europe: I. Asian tiger mosquito (Aedes albopictus)

Author

Igor Pajovic, Elena Fălcuţă, Dušan Petrić, Francis Schaffner, Eleonora Flacio, Carla A. Sousa, Claudio Venturelli, Luciano Toma, Carles Aranda, Enkelejda Velo, Bulent Alten, Norbert Becker, Dimitrios P. Papachristos, Ognyan Mikov, Marco Di Luca, Ana Klobučar, Christophe Lagneau, Marlen I. Vasquez, Arjan Stroo, Marco Carrieri, Paola Angelini, Marija Zgomba, Antonios Michaelakis, Enrih Merdić, and Romeo Bellini

Subjects
medicine.medical_specialty, Insecticide resistance, Aedes albopictus, Mosquito Control, Range (biology), 030231 tropical medicine, Infection control, Plan (drawing), Mosquito Vectors, Emergency treatment, medicine.disease_cause, Medical and Health Sciences, Dengue fever, Dengue, 03 medical and health sciences, 0302 clinical medicine, Aedes, Health Sciences, medicine, Animals, 030212 general & internal medicine, Chikungunya, Environmental planning, Insecticide, Management plan, invasive mosquitoes, Aedes albopictus, biology, Public health, Community participation, Yellow fever, Public Health, Environmental and Occupational Health, biology.organism_classification, medicine.disease, Europe, Infectious Diseases, Geography, Tiger mosquito, Introduced Species
Abstract

Aedes albopictus, also known as the “Asian Tiger Mosquito”, is an invasive mosquito species to Europe causing high concern in public health due to its severe nuisance and its vectorial capacity for pathogens such as dengue, chikungunya, yellow fever and Zika. Consequently, the responsible authorities implement management activities to reduce its population density, possibly to below noxious and epidemiological thresholds. In urban areas, these aims are difficult to achieve because of the species’ ability to develop in a wide range of artificial breeding sites, mainly private properties. This document (Management Plan) has been structured to serve as a comprehensive practical and technical guide for stakeholders in organizing the vector control activities in the best possible way. The current plan includes coordinated actions such as standardized control measures and quality control activities, monitoring protocols, activities for stakeholders and local communities, and an emergency vector control plan to reduce the risk of an epidemic.

Published
2020
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8. Vectopole Sud, a french network of platforms for research in medical, veterinary and agricultural entomology

Author

Thierry Baldet, Fabrice Chandre, Isabelle Deme, Magali Eychenne, Katia Grucker, Karine Huber, Christophe Lagneau, Thierry Lefrancois, Sophie Ravel, Bethsabée Scheid, Marie-Laure Setier-Rio, Olivier Adrot, Philippe Solano, Océane Trevennec, Anne-Nathalie Volkoff, Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM), Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), Entente Interdépartementale pour la Démoustication du Littoral Méditerranéen, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides (UMR INTERTRYP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université de Bordeaux (UB), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut de Recherche pour le Développement (IRD)-Université de Bordeaux (UB)

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, [SDV.BA]Life Sciences [q-bio]/Animal biology, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

Six institutional partners, CIRAD, CNRS, EID Méditerranée, INRA, IRD and the Univer-sity of Montpellier have been working together since 2010 on the issue of arthropod vectors of pathogens that cause infectious diseases in humans and animals, and insect pests responsible for damages to crops. This collaboration for over 8 years has resulted in the creation of “Vectopole Sud”, a Montpellier network of platforms dedicated to re-searches on arthropods of medical, veterinary and agricultural importance. The network draws from the infrastructure and expertise of each partner to improve early warning systems, monitoring, control of pests and vectors in order to protect hu-man health, agriculture and livestock production. The network has received continuous funding by the French Government and the Occitanie Region enabling it to modernize its different laboratories and rearing facilities and support the research activities. Vectopole Sud includes four experimental platforms, confined insectariums and associ-ated laboratories, which are open to national and international partners. Overall, the Vec-topole Sud has a network of partners in Europe and tropical regions worldwide, notably through IRD and CIRAD, two French institutions supporting research for development. Vectopole Sud brings together over 400 researchers and technicians from five research units belonging to the Montpellier University of Excellence and Agropolis International scientific communities. It conducts multidisciplinary research and expertise on biolo-gy, public & animal health, ecology, environmental science, genetics, and evolutionary medicine. It contributes to a better environment, animal health and human wellbeing through an integrated One Health approach, by fighting human and animal diseases to ensure more productive and sustainable farming systems (food security). We will present the different platforms of the Vectopole Sud network, its various activi-ties (infrastructures, research, services and training) as well as its different perspectives. www.vectopole-sud.fr

Published
2018

11. Synthesis of retinoid analogues of juvenile hormones

Author

André Yébakima, Lotfi Doumandji, Christophe Lagneau, Armand Lattes, Jean-Pierre Doucet, Hubert Matondo, Annick Doucet-Panaye, James Devillers, Sébastien Estaran, Boudjema Hamada, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Entente Interdépartementale pour la Démoustication du Littoral Méditerranéen, Centre Traitement Informatique Scientifique (CTIS), Centre de la Démoustication, Conseil General de la Martinique, Interfaces, Traitements, Organisation et Dynamique des Systèmes (ITODYS (UMR_7086)), Université Paris Diderot - Paris 7 (UPD7)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Interactions moléculaires et réactivité chimique et photochimique (IMRCP), Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Fédération de Recherche Fluides, Energie, Réacteurs, Matériaux et Transferts (FERMAT), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)

Subjects
010405 organic chemistry, medicine.drug_class, Chemistry, Retinoic acid, General Chemistry, 010402 general chemistry, 01 natural sciences, 3. Good health, 0104 chemical sciences, Inorganic Chemistry, chemistry.chemical_compound, Biochemistry, Mosquito larvae, Juvenile hormone, Trifluoromethyl ketone, medicine, [CHIM]Chemical Sciences, Mitsunobu reaction, Retinoid, ComputingMilieux_MISCELLANEOUS
Abstract

As part of a collaborative research project aimed at designing new chemicals active on mosquito larvae, we sought accessible raw materials and an efficient synthesis method for preparing large amounts of active substances. For this we selected retinoic acid, which has functionality close to that of juvenile hormones. From this molecule we developed ester and trifluoromethyl ketone synthesis that was fast and led to good yields.

Published
2017
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13. Relating fitness to long-term environmental variations in natura

Author

Mylène Weill, Pierrick Labbé, Christophe Lagneau, Thomas Lenormand, Pascal Milesi, Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Université Paul-Valéry - Montpellier 3 (UPVM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut de Recherche pour le Développement (IRD [France-Sud]), 'Personal Protection Against Vectors' working group (PPAV), PPAV working group, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Université Paul-Valéry - Montpellier 3 (UM3)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)

Subjects
0106 biological sciences, 0301 basic medicine, Insecticides, Population genetics, Locus (genetics), Biology, 010603 evolutionary biology, 01 natural sciences, adaptation dynamics, Evolution, Molecular, 03 medical and health sciences, Genetics, Animals, Allele, Alleles, Ecology, Evolution, Behavior and Systematics, Continuous evolution, population genetics model, Ecology, long-term survey, insecticide resistance, Culex, Mosquito control, Genetics, Population, 030104 developmental biology, Insecticide resistance, [SDE]Environmental Sciences, selective pressure variations, Evolutionary ecology, France, Genetic Fitness, [SDE.BE]Environmental Sciences/Biodiversity and Ecology
Abstract

International audience; Quantifying links between ecological processes and adaptation dynamics in natura remains a crucial challenge. Many studies have documented the strength, form and direction of selection, and its variations in space and time, but only a few managed to link these variations to their proximal causes. This step is, however, crucial, if we are to understand how the variation in selective pressure affects adaptive allele dynamics in natural settings. We used data from a long-term survey (about 30 years) monitoring the adaptation to insecticides of Culex pipiens mosquitoes in Montpellier area (France), focusing on three resistance alleles of the Ester locus. We used a population genetics model taking temporal and spatial variations in selective pressure into account, to assess the quantitative relationships between variations in the proximal agent of selection (amounts of insecticide sprayed) and the fitness of resistance alleles. The response to variations in selective pressure was fast, and the alleles displayed different fitness-to-environment relationships: the analyses revealed that even slight changes in insecticide doses could induce changes in the strength and direction of selection, thus changing the fitness ranking of the adaptive alleles. They also revealed that selective pressures other than the insecticides used for mosquito control affected the resistance allele dynamics. These fitness-to-environment relationships, fast responses and continuous evolution limit our ability to predict the outcome of adaptive allele dynamics in a changing environment, but they clearly contribute to the maintenance of polymorphism in natural populations. Our study also emphasizes the necessity of long-term surveys in evolutionary ecology.

Published
2016
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14. Gender bias in insecticide susceptibility of Aedes albopictus is solely attributable to size

Author

Didier Fontenille, Reda Tounsi, Paul Reiter, Marie Rossignol, Saïd C. Boubidi, Fabrice Chandre, Christophe Lagneau, EID Méditerranée (EID Méditerranée), Etablissement public administratif, Institut Pasteur d'Algérie, Réseau International des Instituts Pasteur (RIIP), Vector Control Group (MIVEGEC-VCG), Evolution des Systèmes Vectoriels (ESV), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Institut Pasteur du Cambodge, Insectes et Maladies Infectieuses, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Insecticides, Aedes albopictus, Mosquito Control, 030231 tropical medicine, sterile insect technique, [STAT.OT]Statistics [stat]/Other Statistics [stat.ML], 01 natural sciences, World health, mosquito control, Toxicology, 03 medical and health sciences, Sterile insect technique, 0302 clinical medicine, Sex Factors, Aedes, Nitriles, Pyrethrins, Gender bias, male size, Dose effect, Bioassay, Animals, Body Size, Ecology, Evolution, Behavior and Systematics, biology, [SDV.BA]Life Sciences [q-bio]/Animal biology, insecticide susceptibility, Significant difference, Public Health, Environmental and Occupational Health, General Medicine, biology.organism_classification, 3. Good health, 010602 entomology, Mosquito control, Insect Science
Abstract

International audience; Despite an extensive literature on mosquitoes, remarkably little attention has been paid to males. Current interest in control by release of transgenic males begs attention to this bias. It is well known that males are more susceptible to insecticides than females when determined by the standard World Health Organization (WHO) bioassay, and field observations have shown a higher impact of ultra-low-volume (ULV) space sprays. It is generally assumed that these differences are due to the smaller size of males and/or greater physiological susceptibility. We compared susceptibility by WHO bioassay and by topical application. There was a significant difference between the sexes in terms of dose effect and knockdown by the WHO test, but no significant difference by weight-adjusted topical application. We conclude that greater susceptibility of males is solely a function of their size and suggest that a ULV treatment before the release of transgenic males would greatly increase their competitive ratio versus wild mosquitoes and thus their impact as a control measure.

Published
2016
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15. Efficacy of ULV and thermal aerosols of deltamethrin for control of Aedes albopictus in Nice, France

Author

Charles Tizon, Romain Benoit, Marie Rossignol, Bernard Cadiou, Didier Fontenille, David Roiz, Reda Tounsi, Saïd C. Boubidi, Marc Raselli, Christophe Lagneau, Paul Reiter, Fabrice Chandre, EID Méditerranée (EID Méditerranée), Etablissement public administratif, Institut Pasteur d'Algérie, Réseau International des Instituts Pasteur (RIIP), Vector Control Group (MIVEGEC-VCG), Evolution des Systèmes Vectoriels (ESV), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), EID Méditerranée, Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Institut Pasteur du Cambodge, Insectes et Maladies Infectieuses, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), The research was funded by the European Commission under the 7th Framework, Grant Agreement Number: 282589, We would like to thank Thierry Baldet of IDRC/CRDI–Ottawa, Canada and Annelies Wilder-Smith of University of Umea, Umea, Sweden for useful discussions and advices for the work. Our thanks also to Xavier Allen of CIRAD, UMR15 CMAEE, INRA, Montpellier, France for providing blood for the mosquito rearing and Arnaud Cannet and Pascal Delaunay of Inserm U1065, Centre Méditerranéen de Médecine Moléculaire, C3 M, Université de Nice-Sophia Antipolis for their technical help and all the staff of EID Méditerranée, Montpellier, France for their help in field trials., European Project: 282589,EC:FP7:HEALTH,FP7-HEALTH-2011-single-stage,DENGUETOOLS(2011), and Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)

Subjects
Insecticides, Veterinary medicine, Mosquito Control, Aedes albopictus, 030231 tropical medicine, Thermal fogging, Context (language use), Aedes aegypti, medicine.disease_cause, lcsh:Infectious and parasitic diseases, Toxicology, Dengue, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, ULV, Aedes, Nitriles, Pyrethrins, parasitic diseases, medicine, Animals, lcsh:RC109-216, 030212 general & internal medicine, Chikungunya, Cold fogging, Aerosols, biology, Research, fungi, Temperature, biology.organism_classification, Vector control, 3. Good health, Mosquito control, Infectious Diseases, Deltamethrin, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, chemistry, Fogger, Biological Assay, Parasitology, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, France
Abstract

Background Ultra-low volume (ULV) insecticidal aerosols dispensed from vehicle-mounted cold-foggers are widely considered the method of choice for control of Aedes aegypti and Ae. albopictus during outbreaks of dengue and chikungunya and, more recently, Zika. Nevertheless, their effectiveness has been poorly studied, particularly in Europe. Nearly all published studies of ULV efficacy are bio-assays based on the mortality of caged mosquitoes. In our study we preferred to monitor the direct impact of treatments on the wild mosquito populations. This study was undertaken to evaluate the efficiency of the two widely used space spraying methods to control Ae. albopictus and Ae. aegypti. Methods We determined the susceptibility of local Ae. albopictus to deltamethrin by two methods: topical application and the “WHO Tube Test”. We used ovitraps baited with hay infusion and adult traps (B-G Sentinel) baited with a patented attractant to monitor the mosquitoes in four residential areas in Nice, southern France. The impact of deltamethrin applied from vehicle-mounted ULV fogging-machines was assessed by comparing trap results in treated vs untreated areas for 5 days before and 5 days after treatment. Four trials were conducted at the maximum permitted application rate (1 g.ha-1). We also made two small-scale tests of the impact of the same insecticide dispensed from a hand-held thermal fogger. Results Susceptibility to the insecticide was high but there was no discernable change in the oviposition rate or the catch of adult female mosquitoes, nor was there any change in the parous rate. In contrast, hand-held thermal foggers were highly effective, with more than 90% reduction of both laid eggs and females. Conclusions We believe that direct monitoring of the wild mosquito populations gives a realistic assessment of the impact of treatments and suggest that the lack of efficacy is due to lack of interaction between the target mosquitoes and the ULV aerosol. We discuss the factors that influence the effectiveness of both methods of spraying in the context of epidemic situations. Electronic supplementary material The online version of this article (doi:10.1186/s13071-016-1881-y) contains supplementary material, which is available to authorized users.

Published
2016
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16. The mothHylesia metabusand French Guiana lepidopterism: centenary of a public health concern

Author

Christophe Lagneau, Jean-Michel Vassal, Fabrice Chandre, Romain Girod, Frédéric Jourdain, Vincent Robert, Florence Fouque, Jocelyn Raude, Daniel Guiral, Centre National d'Expertise sur les Vecteurs, Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur (RIIP), Bioagresseurs, analyse et maîtrise du risque (UPR Bioagresseurs), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), EID Méditerranée, EID, Institut Pasteur de la Guadeloupe, Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), École des Hautes Études en Santé Publique [EHESP] (EHESP), Département des sciences humaines et sociales (SHS), Diversity, ecology, evolution & Adaptation of arthropod vectors (MIVEGEC-DEEVA), Evolution des Systèmes Vectoriels (ESV), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Institut Pasteur de la Guyane Française, Institut Pasteur de la Guyane - Réseau International des Instituts Pasteur, CIRAD, Campus international de Baillarguet, Université de Montpellier (UM) - Centre National de la Recherche Scientifique (CNRS) - Institut de Recherche pour le Développement (IRD [France-Sud]), Réseau International des Instituts Pasteur - Institut Pasteur de la Guadeloupe, Institut Méditerranéen d'Ecologie et de Paléoécologie (IMEP), and Université Paul Cézanne - Aix-Marseille 3 - Université de Provence - Aix-Marseille 1 - Université d'Avignon et des Pays de Vaucluse (UAPV) - Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, 0106 biological sciences, Dermatitis, Ectoparasitic Infestations, Moths, 01 natural sciences, MESH: Ectoparasitic Infestations, MESH: Insect Control, 0302 clinical medicine, nuisance, MESH: Animals, yellowtail moth dermatitis, dermite, biology, MESH: Moths, Ecology, mangrove swamp, papillonite, LUTTE ANTIINSECTE, French Guiana, 3. Good health, Lepidoptera, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Infectious Diseases, pullulation, S50 - Santé humaine, Female, dermatite, lepidopterism, Nuisance, ECOLOGIE, infestation, medicine.medical_specialty, Hylesia metabus, Veterinary (miscellaneous), 030231 tropical medicine, review, MANGROVE, Insect Control, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, Guyane française, SURVEILLANCE, MESH: French Guiana, medicine, Animals, Humans, INSECTE NUISIBLE, lcsh:RC109-216, MESH: Dermatitis, PAPILLONITE, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, papillon cendre, mangrove, MESH: Humans, urticaire, Public health, BIOLOGIE, urticariad, biology.organism_classification, medicine.disease, revue, MESH: Male, 010602 entomology, Lepidopterism, Insect Science, Yellowtail moth, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Animal Science and Zoology, Parasitology, MESH: Female
Abstract

International audience; The females of the moths Hylesia metabus have their abdomens covered by urticating hairs looking like micro-arrows and causing a puriginous dermatitis to humans known as "papillonite" in French Guiana and also called yellowtail moth dermatitis or Caripito itch. The densities of the moths show great seasonal and annual variations depending on mechanisms mostly unknown. When H. metabus infestations occur, numerous cases of dermatologic manifestations are reported from people living near the mangrove swamps where the moths are developing. One hundred years after the first "papillonite" epidemic reported from French Guiana in 1912, the data presented herein summarize the actual state of knowledge on H. metabus biology and ecology and on the lepidopterism. Some recommendations are proposed for the surveillance and warning systems of H. metabus infestations and to avoid contact with the moths. Research priorities are suggested to improve the control against this problem emerging between nuisance and public health.

Published
2012
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17. No association between the use of Bti for mosquito control and the dynamics of non-target aquatic invertebrates in French coastal and continental wetlands: No association between the use of Bti for mosquito control and the dynamics of non-target aquatic invertebrates in French coastal and continental wetlands

Author

Eduard Szöcs, Christophe Lagneau, Céline Bertrand, Benoît Francés, Laurent Lagadic, Benoit Le Hunsec, Stéphanie Fayolle, Yves Rozier, Evelyne Franquet, Ralf B. Schäfer, Sébastien Chouin, Claire Duchet, Marc Roucaute, Rémi Foussadier, Jérôme de Maupeou, Jean-Baptiste Santoni, Écologie et santé des écosystèmes (ESE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institute of Environmental Sciences [Landau], University of Koblenz-Landau, Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), EID Rhône-Alpes, 'Personal Protection Against Vectors' working group (PPAV), PPAV working group, AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
Bacillus thuringiensis israelensis, 0106 biological sciences, Insecticides, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Environmental Engineering, Mosquito Control, Aquatic non-target invertebrates, Wetland, 010501 environmental sciences, Biology, 010603 evolutionary biology, 01 natural sciences, Hemolysin Proteins, Chironomids, Bacterial Proteins, Environmental monitoring, Environmental Chemistry, Animals, Ecosystem, Pest Control, Biological, Waste Management and Disposal, 0105 earth and related environmental sciences, Invertebrate, geography, geography.geographical_feature_category, Bacillus thuringiensis Toxins, [SDE.IE]Environmental Sciences/Environmental Engineering, Ecology, Flooding (psychology), Pollution, Invertebrates, Endotoxins, Mosquito control, Habitat, Wetlands, France, Biopesticide, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Field biomonitoring, Environmental Monitoring
Abstract

International audience; The environmental safety of Bacillus thuringiensis subsp. israelensis (Bti) is still controversial, mainly because most of the previous field studies on its undesired effects were spatially limited and did not address the relationship between community similarity and application time and frequency. No general statement can therefore be drawn on the usage conditions of Bti that insure protection of non-target organisms. The present study was conducted in eight sites distributed over the main geographical sectors where mosquito control is implemented in mainland France and Corsica. Changes in non-target aquatic invertebrates were followed at elapsed time after repeated applications of two Bti formulations (VectoBac (R) WDG or 12AS) up to four consecutive years. We examined the influence of both larvicide treatments and environmental variables on community dynamics and dissimilarity between treated and control areas. As it can be argued that chironomids are the most vulnerable group of non-target invertebrates, we scrutinised potential Bti-related effects on the dynamics of their community. The use of VectoBac (R) WDG and 12AS in coastal and continental wetlands had no immediate or long-term detectable effect on the taxonomic structure and taxa abundance of non-target aquatic invertebrate communities, including chironomids. This applied to the main habitats where mosquito larvae occur, regardless of their geographic location. Flooding, whose frequency and duration depend on local meteorological and hydrological conditions, was identified as the main environmental driver of invertebrate community dynamics. Our findings add support to the environmental safety of currently available Bti formulations when following recommended application rates and best mosquito control practices. (C) 2016 Elsevier B.V. All rights reserved.

Published
2015
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18. Effects of Bacillus thuringiensis israelensis and spinosad on adult emergence of the non-biting midges Polypedilum nubifer (Skuse) and Tanytarsus curticornis Kieffer (Diptera: Chironomidae) in coastal wetlands

Author

Christophe Lagneau, Evelyne Franquet, Claire Duchet, Laurent Lagadic, Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU), Écologie et santé des écosystèmes (ESE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), 'Personal Protection Against Vectors' working group (PPAV), PPAV working group, and Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Nematocera, Insecticides, Mosquito Control, Health, Toxicology and Mutagenesis, Population, Population Dynamics, Bacillus thuringiensis, Spinosad, Non-target invertebrate, 010501 environmental sciences, 010603 evolutionary biology, 01 natural sciences, Chironomidae, parasitic diseases, medicine, Animals, 14. Life underwater, education, Larvicide, Tanytarsus, 0105 earth and related environmental sciences, education.field_of_study, biology, Ecology, fungi, Public Health, Environmental and Occupational Health, General Medicine, Bacillus thuringiensis israelensis, biology.organism_classification, Pollution, Mosquito control, Drug Combinations, Non-biting midge, Biological insecticide, Wetlands, Field enclosure, Macrolides, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, medicine.drug
Abstract

International audience; To optimize their efficacy, some insecticides used for mosquito control are introduced into aquatic ecosystems where mosquito larvae develop (marshes, ponds, sanitation devices) and cannot escape from the treated water. However, this raises the question of possible effects of mosquito larvicides on nontarget aquatic species. Bacillus thuringiensis var. israelensis (Bti), which is well-known for its selectivity for Nematocera dipterans, is widely used for mosquito control all over the world. Spinosad, a mixture of spinosyns A and D known as fermentation products of a soil actinomycete (Saccharopolyspora spinosa), is a biological neurotoxic insecticide with a broader action spectrum. It is a candidate larvicide for mosquito control, but some studies showed that it may be toxic to beneficial or non-target species, including non-biting midges. The present study was therefore undertaken to assess the impact of Bti and spinosad on natural populations of Polypedilum nubifer (Skuse) and Tanytarsus curticornis Kieffer (Diptera: Chironomidae) in field enclosures implemented in Mediterranean coastal wetlands. Unlike Bti, spinosad had a strong lethal effect on Polypedilum nubifer and seems to affect Tanytarsus curticornis at presumed recommended rates for field application. Differences in the sensitivity of these two species to spinosad confirm that population dynamics need to be known for a proper assessment of the risk encountered by chironomids in wetlands where larvicide-based mosquito control occurs. (C) 2015 Elsevier Inc. All rights reserved.

Published
2014
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19. Persistence and Recycling of Bioinsecticidal Bacillus thuringiensis subsp. israelensis Spores in Contrasting Environments: Evidence from Field Monitoring and Laboratory Experiments

Author

Claire Duchet, Christophe Lagneau, Laurence Després, Margot Paris, Delphine Rey, Albane Marie, Jean-Philippe David, Yvon Perrin, Guillaume Tetreau, Gilles Besnard, Écologie et santé des écosystèmes (ESE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Laboratoire d'Ecologie Alpine (LECA), Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Joseph Fourier - Grenoble 1 (UJF)-Université Grenoble Alpes (UGA), Entente Interdépartementale pour la Démoustication du Littoral Méditerranéen, Centre national d'expertise des vecteurs, Montpellier (CNEV), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Joseph Fourier - Grenoble 1 (UJF), Institut National de L'Energie Solaire (INES), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Mécanique et Technologie (LMT), École normale supérieure - Cachan (ENS Cachan)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Aménagement, Développement, Environnement, Santé et Sociétés (ADES), Centre National de la Recherche Scientifique (CNRS)-Université Bordeaux Segalen - Bordeaux 2-Université Bordeaux Montaigne, Laboratoire de Biologie des Populations d'Altitude, Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), and Université Bordeaux Segalen - Bordeaux 2-Université Bordeaux Montaigne-Centre National de la Recherche Scientifique (CNRS)

Subjects
Mosquito Control, [SDV]Life Sciences [q-bio], Bacillus thuringiensis, Soil Science, Environment, recycling, Persistence (computer science), mosquito larvae, [SDV.EE.ECO]Life Sciences [q-bio]/Ecology, environment/Ecosystems, Botany, parasitic diseases, Animals, Organic matter, Ecology, Evolution, Behavior and Systematics, ComputingMilieux_MISCELLANEOUS, chemistry.chemical_classification, Spores, Bacterial, Bacillus thuringiensis subsp israelensis, geography, geography.geographical_feature_category, Ecology, biology, fungi, persistence, 15. Life on land, biology.organism_classification, field, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Spore, Salinity, Horticulture, Mosquito control, Culicidae, chemistry, Brackish marsh, Larva, [SDE]Environmental Sciences, France, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Microcosm
Abstract

Sprays of commercial preparations of the bacterium Bacillus thuringiensis subsp. israelensis are widely used for the control of mosquito larvae. Despite an abundant literature on B. thuringiensis subsp. israelensis field efficiency on mosquito control, few studies have evaluated the fate of spores in the environment after treatments. In the present article, two complementary experiments were conducted to study the effect of different parameters on B. thuringiensis subsp. israelensis persistence and recycling, in field conditions and in the laboratory. First, we monitored B. thuringiensis subsp. israelensis persistence in the field in two contrasting regions in France: the Rhone-Alpes region, where mosquito breeding sites are temporary ponds under forest cover with large amounts of decaying leaf matter on the ground and the Mediterranean region characterized by open breeding sites such as brackish marshes. Viable B. thuringiensis subsp. israelensis spores can persist for months after a treatment, and their quantity is explained both by the vegetation type and by the number of local treatments. We found no evidence of B. thuringiensis subsp. israelensis recycling in the field. Then, we tested the effect of water level, substrate type, salinity and presence of mosquito larvae on the persistence/recycling of B. thuringiensis subsp. israelensis spores in controlled laboratory conditions (microcosms). We found no effect of change in water level or salinity on B. thuringiensis subsp. israelensis persistence over time (75 days). B. thuringiensis subsp. israelensis spores tended to persist longer in substrates containing organic matter compared to sand-only substrates. B. thuringiensis subsp. israelensis recycling only occurred in presence of mosquito larvae but was unrelated to the presence of organic matter.

Published
2014
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20. Chapter 11Receptor-Guided Structure–Activity Modeling of Inhibitors of Juvenile Hormone Epoxide Hydrolases

Author

Robert Delorme, Laurent Lagadic, André Yébakima, O. Yamada, Bruno Lapied, Frédérique Darriet, James Devillers, Christophe Lagneau, X Deparis, Jean-Philippe Jaege, and Françoise Quiniou

Subjects
010404 medicinal & biomolecular chemistry, Mathematical optimization, Vector control, 010405 organic chemistry, Computer science, Multicriteria analysis, 01 natural sciences, 0104 chemical sciences
Published
2013
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21. DengueTools: innovative tools and strategies for the surveillance and control of dengue

Author

Sazaly Abu Bakar, Christoph Hatz, Yesim Tozan, David Brooks, Steve W. Lindsay, Joacim Rocklöv, Johannah Wegerdt, Eduardo Massad, Hasitha Tissera, Annelies Wilder-Smith, Karl Erik Renhorn, Antonio Tenorio, Duane J. Gubler, Grégory L'Ambert, Pattamaporn Kittayapong, Valérie R. Louis, Paul Reiter, Peter Byass, Christophe Lagneau, James G. Logan, Luke Alphey, European Commission, University of Zurich, and Wilder-Smith, Annelies

Subjects
Economic growth, economic evaluation, International Cooperation, Public Health, Global Health, Prevention, Dengue fever, Disease Outbreaks, 0302 clinical medicine, Medicine, Globalization, 030212 general & internal medicine, 2. Zero hunger, education.field_of_study, Dengue, early warning, surveillance, preventive strategies, Intervention, Asia, Europe, Warning system, Study Design Article, Health Policy, lcsh:Public aspects of medicine, Incidence, Public Health, Global Health, Social Medicine and Epidemiology, 3. Good health, climate change, Population Surveillance, Risk assessment, Epidemiology, Medical geography, 030231 tropical medicine, Population, Context (language use), 610 Medicine & health, early warning systems, Health Promotion, entomology, dengue diagnostics, Risk Assessment, 03 medical and health sciences, Arbetsmedicin och miljömedicin, Urbanization, Humans, education, risk mapping, business.industry, Public Health, Environmental and Occupational Health, lcsh:RA1-1270, Occupational Health and Environmental Health, 10060 Epidemiology, Biostatistics and Prevention Institute (EBPI), 2739 Public Health, Environmental and Occupational Health, medicine.disease, 2719 Health Policy, dengue, Folkhälsovetenskap, global hälsa, socialmedicin och epidemiologi, Health promotion, Public Health Practice, Diffusion of Innovation, business, dengue control, globalization
Abstract

Dengue fever is a mosquito-borne viral disease estimated to cause about 230 million infectionsworldwide every year, of which 25,000 are fatal. Global incidence has risen rapidly in recent decades with some 3.6 billion people, over half of the world’s population, now at risk, mainly in urban centres of the tropics and subtropics. Demographic and societal changes, in particular urbanization, globalization, and increased international travel, are major contributors to the rise in incidence and geographic expansion of dengue infections. Major research gaps continue to hamper the control of dengue. The European Commission launched a call under the 7th Framework Programme with the title of ‘Comprehensive control of Dengue fever under changing climatic conditions’. Fourteen partners from several countries in Europe, Asia, and South America formed a consortium named ‘DengueTools’ to respond to the call to achieve better diagnosis, surveillance, prevention, and predictive models and improve our understanding of the spread of dengue to previously uninfected regions (including Europe) in the context of globalization and climate change. The consortium comprises 12 work packages to address a set of research questions in three areas: Research area 1: Develop a comprehensive early warning and surveillance system that has predictive capability for epidemic dengue and benefits from novel tools for laboratory diagnosis and vector monitoring. Research area 2: Develop novel strategies to prevent dengue in children. Research area 3: Understand and predict the risk of global spread of dengue, in particular the risk of introduction and establishment in Europe, within the context of parameters of vectorial capacity, global mobility, and climate change. In this paper, we report on the rationale and specific study objectives of ‘DengueTools’. DengueTools is funded under the Health theme of the Seventh Framework Programme of the European Community, Grant Agreement Number: 282589 Dengue Tools.Keywords: dengue; dengue control; dengue diagnostics; economic evaluation; entomology; climate change; early warning systems; risk mapping; surveillance; globalization(Published: 22 March 2012)Citation: Glob Health Action 2012, 5: 17273 - DOI: 10.3402/gha.v5i0.17273

Published
2012
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22. Chitobiase activity as an indicator of altered survival, growth and reproduction in Daphnia pulex and Daphnia magna (Crustacea: Cladocera) exposed to spinosad and diflubenzuron

Author

Claire Duchet, Thierry Caquet, Michel Larroque, Christophe Lagneau, Evelyne Franquet, Laurent Lagadic, Marília Mitie Inafuku, Entente Interdépartementale pour la Démoustication du Littoral Méditerranéen, Écologie et santé des écosystèmes (ESE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Démarche intégrée pour l'obtention d'aliments de qualité (UMR Qualisud), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Université de La Réunion (UR)-Université de Montpellier (UM)-Avignon Université (AU)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1), Institut Méditerranéen d'Écologie et de Paléoécologie, Université Paul Cézanne - Aix-Marseille 3, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Avignon Université (AU)-Université de La Réunion (UR)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)

Subjects
0106 biological sciences, Insecticides, Health, Toxicology and Mutagenesis, [SDV]Life Sciences [q-bio], Spinosad, 010501 environmental sciences, 01 natural sciences, Daphnia, Toxicology, chemistry.chemical_compound, Chitobiase, Neurotoxicant, biology, Reproduction, General Medicine, Bti, Environmental risk assessment, Pollution, 3. Good health, Drug Combinations, Diflubenzuron, Cladocera, Daphnia sp, Female, Macrolides, Moulting, medicine.drug, Adult, Population dynamics, Daphnia magna, Bacillus thuringiensis, Insect growth regulator, Daphnia pulex, Risk Assessment, Animal science, Acetylglucosaminidase, medicine, Animals, Humans, 0105 earth and related environmental sciences, Bioindicator, 010604 marine biology & hydrobiology, Public Health, Environmental and Occupational Health, Infant, Newborn, biology.organism_classification, Pulex, chemistry, 13. Climate action, Biomarkers, Water Pollutants, Chemical
Abstract

Chitobiase is involved in exoskeleton degradation and recycling during the moulting process in arthropods. In aquatic species, the moulting fluid is released into the aqueous environment, and chitobiase activity present therein can be used to follow the dynamics of arthropod populations. Here, chitobiase activity was used for monitoring the impact of mosquito candidate larvicides on Daphnia pulex and Daphnia magna under laboratory conditions. Both species were exposed to spinosad (2, 4, 8 mu g L-1) and diflubenzuron (0.2, 0.4, 0.8 mu g L-1) for 14 days. Bacillus thuringiensis var. israelensis (Bti: 0.25, 0.5, 1 mu L L-1) was used as the reference larvicide. Chitobiase activity, adult survival, individual growth and fecundity, expressed as the number of neonates produced, were measured every 2 days. Average Exposure Concentrations of spinosad were ten-fold lower than the nominal concentrations, whereas only a slight deviation was observed for diflubenzuron. In contrast to Bti, spinosad and diflubenzuron significantly affected both species in terms of adult survival, and production of neonates. As compared to D. pulex, D. magna was more severely affected by diflubenzuron, at low and medium concentrations, with reduced adult growth and much lower chitobiase activity. Chitobiase activity was positively correlated with the individual body length, number of neonates produced between two consecutive observation dates, and number of females and neonates. In addition, the significant positive correlations between chitobiase activity measured on the last sampling date before the first emission of neonates and the cumulative number of neonates produced during the whole observation period strongly support the potential of the activity of this chitinolytic enzyme as a proxy for assessing the dynamics of arthropod populations exposed to larvicides used for mosquito control. (C) 2010 Elsevier Inc. All rights reserved.

Published
2011
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23. Population-level effects of spinosad and Bacillus thuringiensis israelensis in Daphnia pulex and Daphnia magna: comparison of laboratory and field microcosm exposure conditions

Author

Laurent Lagadic, Marie-Agnès Coutellec, Claire Duchet, Christophe Lagneau, Evelyne Franquet, Écologie et santé des écosystèmes (ESE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut Méditerranéen d'Ecologie et de Paléoécologie (IMEP), Université Paul Cézanne - Aix-Marseille 3-Centre National de la Recherche Scientifique (CNRS)-Avignon Université (AU)-Université de Provence - Aix-Marseille 1, Entente Interdépartementale pour la Démoustication du Littoral Méditerranéen, French Ministry for Ecology, Ecotoxicology (PNETOX), and Université Paul Cézanne - Aix-Marseille 3-Université de Provence - Aix-Marseille 1-Avignon Université (AU)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Insecticides, Biopesticides, Health, Toxicology and Mutagenesis, [SDV]Life Sciences [q-bio], Population, Daphnia magna, Bacillus thuringiensis, Spinosad, 010501 environmental sciences, Management, Monitoring, Policy and Law, Toxicology, 01 natural sciences, Daphnia pulex, Laboratory, medicine, Animals, Life Tables, Population Growth, education, 0105 earth and related environmental sciences, Stochastic Processes, education.field_of_study, Population extinction risk, biology, General Medicine, Bacillus thuringiensis israelensis, Mosquito control, biology.organism_classification, Survival Analysis, Drug Combinations, 010602 entomology, Pulex, Stochastic model, Daphnia, Cladocera, 13. Climate action, Larva, Laboratory In situ microcosms, Female, Macrolides, Microcosm, medicine.drug
Abstract

Because exposure to toxicants not only results in mortality but also in multiple sublethal effects, the use of life-table data appears particularly suitable to assess global effects on exposed populations. The present study uses a life table response approach to assess population-level effects of two insecticides used against mosquito larvae, spinosad (8 mu g/l) and Bacillus thuringiensis var. israelensis (Bti, 0.5 mu l/l), on two non target species, Daphnia pulex and Daphnia magna (Crustacea: Cladocera), under laboratory versus field microcosms conditions. Population growth rates were inferred from life table data and Leslie matrices under a model with resource limitation (ceiling). These were further used to estimate population risks of extinction under each tested condition, using stochastic simulations. In laboratory conditions, analyses performed for each species confirmed the significant negative effect of spinosad on survival, mean time at death, and fecundity as compared to controls and Bti-treated groups; for both species, population growth rate lambda was lower under exposure to spinosad. In field microcosms, 2 days after larvicide application, differences in population growth rates were observed between spinosad exposure conditions, and control and Bti exposure conditions. Simulations performed on spinosad-exposed organisms led to population extinction (minimum abundance = 0, extinction risk = 1), and this was extremely rapid (time to quasi-extinction = 4.1 one-week long steps, i.e. one month). Finally, D. magna was shown to be more sensitive than D. pulex to spinosad in the laboratory, and the effects were also detectable through field population demographic simulations.

Published
2010
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24. Influence of environmental factors on the response of a natural population of Daphnia magna (Crustacea: Cladocera) to spinosad and Bacillus thuringiensis israelensis in Mediterranean coastal wetlands

Author

Laurent Lagadic, Claire Duchet, Evelyne Franquet, Thierry Caquet, Christophe Lagneau, Écologie et santé des écosystèmes (ESE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Entente Interdépartementale pour la Démoustication du Littoral Méditerranéen, Institut Méditerranéen d'Ecologie et de Paléoécologie (IMEP), Université Paul Cézanne - Aix-Marseille 3-Université de Provence - Aix-Marseille 1-Avignon Université (AU)-Centre National de la Recherche Scientifique (CNRS), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and Université Paul Cézanne - Aix-Marseille 3-Centre National de la Recherche Scientifique (CNRS)-Avignon Université (AU)-Université de Provence - Aix-Marseille 1

Subjects
0106 biological sciences, Insecticides, SALINITE, DAPHNIE, Health, Toxicology and Mutagenesis, DAPHNIA, Spinosad, Fresh Water, 010501 environmental sciences, Toxicology, 01 natural sciences, Daphnia, BIOPESTICIDE, SALINITY, TEMPERATURE, reproductive and urinary physiology, biology, Mediterranean Region, Ecology, General Medicine, Bacillus thuringiensis israelensis, Pollution, Drug Combinations, Cladocera, Natural population growth, CRUSTACEA, Larva, Macrolides, Microcosm, STRESSOR, medicine.drug, Daphnia magna, CLADOCERA, Bacillus thuringiensis, medicine, Animals, 14. Life underwater, MICROCOSMS, Pest Control, Biological, Larvicide, 0105 earth and related environmental sciences, 010604 marine biology & hydrobiology, fungi, MICROCOSM, mer méditerranée, FACTEUR D'IMPACT, biology.organism_classification, DAPHNIA MAGNA, [SDE.ES]Environmental Sciences/Environmental and Society, Wetlands
Abstract

The present study was undertaken to assess the impact of a candidate mosquito larvicide, spinosad (8, 17 and 33 μg L−1) on a field population of Daphnia magna under natural variations of water temperature and salinity, using Bti (0.16 and 0.50 μL L−1) as the reference larvicide. Microcosms (125 L) were placed in a shallow temporary marsh where D. magna was naturally present. The peak of salinity observed during the 21-day observation period may have been partly responsible for the decrease of daphnid population density in all the microcosms. It is also probably responsible for the absence of recovery in the microcosms treated with spinosad which caused a sharp decrease of D. magna abundance within the first two days following treatment whereas Bti had no effect. These results suggest that it may be difficult for a field population of daphnids to cope simultaneously with natural (water salinity and temperature) and anthropogenic (larvicides) stressors. Significant interaction between salinity and spinosad exposure impairs the recovery of a natural population of Daphnia magna

Published
2010
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25. Reduced efficacy of pyrethroid space sprays for dengue control in an area of Martinique with pyrethroid resistance

Author

Christophe Lagneau, Alexandre Carron, Manuel Etienne, Michel Tolosa, Sébastien Marcombe, Philip Agnew, Marie Michèle Yp-Tcha, André Yébakima, Frédéric Darriet, and Vincent Corbel

Subjects
Insecticides, Mosquito Control, Pyrethrum, Population, Aedes aegypti, Dengue fever, Toxicology, Dengue, Insecticide Resistance, chemistry.chemical_compound, Aedes, Virology, Naled, parasitic diseases, Pyrethrins, medicine, Animals, Martinique, education, Aerosols, education.field_of_study, Pyrethroid, biology, medicine.disease, biology.organism_classification, Infectious Diseases, Deltamethrin, chemistry, Parasitology, Female
Abstract

In the Caribbean, insecticide resistance is widely developed in Aedes aegypti and represents a serious obstacle for dengue vector control. The efficacy of pyrethroid and organophosphate Ultra-low volume space sprays was investigated in Martinique where Ae. aegypti 6 been shown to be resistant to conventional insecticides. In the laboratory, it wild-field caught Population showed high levels of resistance to deltamethrin, organophosphate (naled), and pyrethrum. Simulated-field trials showed that this resistance call strongly reduce the knock-down effect and mortality of deltamethrin and synergized pyrethrins when applied by thermal-fogging. Conversely., the efficacy of naled was high against insecticide-resistant mosquitoes. Chemical analyses of nettings exposed to the treatments showed a decrease in residues over distance from release for the pyrethroids, and naled was not detected. This finding has important implications for dengue vector control and emphasizes the need to develop innovative strategies to maintain effective control of resistant Ae. aegypti populations.

Published
2009

32. Quelles sont les modalités organisationnelles et les ressources humaines ?

Author

Christophe Lagneau, Didier Fontenille, Régine Lefait Robin, Bernard Tirel, André Yébakima, Sylvie Lecollinet, and Michel Setbon

Abstract

Le livre II du Code rural relatif a la sante publique veterinaire et a la protection des animaux definit une liste des maladies legalement reputees contagieuses (MLRC), certaines a transmission vectorielle, donnant lieu a declaration et a application de mesures de police sanitaire. Certaines MLRC donnent lieu a l'elaboration de plans d'urgence prepares au niveau national par le ministre charge de l'Agriculture et, dans chaque departement, par le prefet. Pour ces maladies, apres leur constatation, le prefet statue sur les mesures a mettre en execution qui peuvent comprendre : « la desinfection et la desinsectisation des ecuries, etables, voitures ou autres moyens de transport, la desinfection ou la destruction des objets, des produits animaux ou d'origine animale susceptibles d'avoir ete contamines et de tout vecteur anime ou inanime pouvant servir de vehicules a la contagion », (article L. 223-8). De plus, dans certains cas definis a l’article L. 224 et pour des

Published
2009
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33. Practices and methods

Author

Christophe Lagneau, Michel Setbon, Bernard Tirel, Didier Fontenille, Régine Lefait Robin, André Yébakima, and Sylvie Lecollinet

Published
2009
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39. Organization and human resources for vector control

Author

André Yébakima, Michel Setbon, Sylvie Lecollinet, Régine Lefait Robin, Christophe Lagneau, Didier Fontenille, and Bernard Tirel

Subjects
Vector control, Knowledge management, business.industry, Computer science, business, Human resources
Published
2009
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41. Annexe 5. Glossaire

Author

Didier Fontenille, Sylvie Lecollinet, André Yébakima, Bernard Tirel, Régine Lefait Robin, Michel Setbon, and Christophe Lagneau

Published
2009
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44. Quelle est la contribution de l’évaluation des risques vectoriels à l’évaluation du risque épidémique ?

Author

André Yébakima, Christophe Lagneau, Sylvie Lecollinet, Bernard Tirel, Régine Lefait Robin, Michel Setbon, and Didier Fontenille

Abstract

Une conference telephonique a ete organisee le 31 juillet 2008 entre les membres du groupe pour discuter de la structuration et du contenu de la reponse sur la base d’un plan de discussion fourni prealablement par J.-C. Desenclos. Suite a cette conference un 2 plan de travail plus detaille a ete diffuse et complete et a servi a la redaction d’un document detaille par J.-C. Desenclos et M. Ledrans.

Published
2009
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47. Appendix 3. Expert group

Author

Michel Setbon, Régine Lefait Robin, Bernard Tirel, Sylvie Lecollinet, Didier Fontenille, Christophe Lagneau, and André Yébakima

Subjects
medicine.medical_specialty, medicine.anatomical_structure, medicine, Medical physics, Psychology, Expert group, Appendix
Published
2009
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