27 results on '"Gao, Cai-rong"'
Search Results
2. Time-dependent expression of SNAT2 mRNA in the contused skeletal muscle of rats: a possible marker for wound age estimation
- Author
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Du, Qiu-xiang, Sun, Jun-hong, Zhang, Ling-yu, Liang, Xin-hua, Guo, Xiang-jie, Gao, Cai-rong, and Wang, Ying-yuan
- Published
- 2013
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3. Screening Biomarkers of Sudden Coronary Death Based on mRNA Expression Profile of Rat Myocardial Tissues.
- Author
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GUO Xiang-jie, LI Hao, BAI Ya-qin, WU Peng, ZHAO Chun-mei, DONG Yi-ming, CHEN Nian-nian, YUN Ke-ming, and GAO Cai-rong
- Subjects
GENE expression ,BIOMARKERS ,FOS oncogenes ,IDENTIFICATION of the dead ,FORENSIC pathology ,SUDDEN death - Abstract
Objective To explore the differential expression of messenger RNA (mRNA) in myocardial tissues of rats with sudden coronary death (SCD), and to provide ideas for the forensic identification of SCD. Methods The rat SCD model was established, and the transcriptome sequencing was performed by next-generation sequencing technology. Differentially expressed genes (DEGs) in myocardial tissues of SCD rats were screened by using the R package limma. A protein-protein interaction (PPI) network was constructed by using the STRING database and Cytoscape 3.8.2 on DEG, and hub genes were screened based on cytoHubba plug-in. Finally, the R package clusterProfiler was used to analyze the biological function and signal pathway enrichment of the selected DEG. Results A total of 177 DEGs were associated with SCD and were mainly involved in the renin-angiotensin system and PI3K-Akt signaling pathway. The genes including angiotensinogen (AGT), complement component 4a (C4a), Fos proto-oncogene (FOS)and others played key roles in the development of SCD. Conclusion Genes such as AGT, C4a, FOS and other genes are expected to be potential biomarkers for forensic identification of SCD. The study based on mRNA expression profile can provide a reference for forensic identification of SCD. [ABSTRACT FROM AUTHOR]
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- 2022
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4. Validation of reference genes for estimating wound age in contused rat skeletal muscle by quantitative real-time PCR
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Sun, Jun-hong, Nan, Liang-huan, Gao, Cai-rong, and Wang, Ying-yuan
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- 2012
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5. Time-dependent expression of skeletal muscle troponin I mRNA in the contused skeletal muscle of rats: a possible marker for wound age estimation
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Sun, Jun-hong, Wang, Ying-yuan, Zhang, Lei, Gao, Cai-rong, Zhang, Lin-zhong, and Guo, Zheng
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- 2010
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6. Sudden cardiac death caused by a primary intimal sarcoma of the left coronary artery
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Jiang, Wei-Xiong, Gao, Cai-rong, Sun, Jun-hong, and Bunai, Yasuo
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- 2009
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7. Temporal changes in Egr-1 and c-fos expression in rat models of myocardial ischemia
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Zhai, Li-Qin, primary, Guo, Xiang-Jie, additional, Li, Ze, additional, Sun, Run-Feng, additional, Jin, Qian-Qian, additional, Liu, Ming-Zhe, additional, Guo, Hua-Lin, additional, and Gao, Cai-Rong, additional
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- 2021
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8. Validation of reference genes for estimating wound age in contused rat skeletal muscle by quantitative real-time PCR
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Sun, Jun-hong, primary, Nan, Liang-huan, additional, Gao, Cai-rong, additional, and Wang, Ying-yuan, additional
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- 2011
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9. Time-dependent expression of skeletal muscle troponin I mRNA in the contused skeletal muscle of rats: a possible marker for wound age estimation
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Sun, Jun-hong, primary, Wang, Ying-yuan, additional, Zhang, Lei, additional, Gao, Cai-rong, additional, Zhang, Lin-zhong, additional, and Guo, Zheng, additional
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- 2009
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10. Sudden cardiac death caused by a primary intimal sarcoma of the left coronary artery
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Jiang, Wei-Xiong, primary, Gao, Cai-rong, additional, Sun, Jun-hong, additional, and Bunai, Yasuo, additional
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- 2008
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11. Urine Metabolites Changes in Acute Myocardial Infarction Rats via Metabolomic Analysis.
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Chen NN, Yu JF, Wu P, Luo L, Bai YQ, Wang LK, Li XQ, Li ZP, Gao CR, and Guo XJ
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- Animals, Rats, Male, Chromatography, High Pressure Liquid, Rats, Sprague-Dawley, Principal Component Analysis, Discriminant Analysis, Mass Spectrometry methods, Niacin metabolism, Niacin urine, Hyperlipidemias metabolism, Niacinamide urine, Niacinamide metabolism, Niacinamide analogs & derivatives, Metabolic Networks and Pathways, ROC Curve, Least-Squares Analysis, Forensic Medicine methods, Metabolome, Myocardial Infarction metabolism, Myocardial Infarction urine, Metabolomics methods, Biomarkers urine, Biomarkers metabolism, Disease Models, Animal
- Abstract
Objectives: To screen biomarkers for forensic identification of acute myocardial infarction (AMI) by non-targeted metabolomic studies on changes of urine metabolites in rats with AMI., Methods: The rat models of the sham surgery group, AMI group and hyperlipidemia + acute myocardial infarction (HAMI) group were established. Ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS) was used to analyze the changes of urine metabolic spectrometry in AMI rats. Principal component analysis, partial least squares-discriminant analysis, and orthogonal partial least squares-discriminant analysis were used to screen differential metabolites. The MetaboAnalyst database was used to analyze the metabolic pathway enrichment and access the predictive ability of differential metabolites., Results: A total of 40 and 61 differential metabolites associated with AMI and HAMI were screened, respectively. Among them, 22 metabolites were common in both rat models. These small metabolites were mainly concentrated in the niacin and nicotinamide metabolic pathways. Within the 95% confidence interval, the area under the curve (AUC) values of receiver operator characteristic curve for N8-acetylspermidine, 3-methylhistamine, and thymine were greater than 0.95., Conclusions: N8-acetylspermidine, 3-methylhistamine, and thymine can be used as potential biomarkers for AMI diagnosis, and abnormal metabolism in niacin and nicotinamide may be the main causes of AMI. This study can provide reference for the mechanism and causes of AMI identification.
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- 2024
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12. Visualization Analysis of Artificial Intelligence Literature in Forensic Research.
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Dong YM, Zhao CM, Chen NN, Luo L, Li ZP, Wang LK, Li XQ, Ren TG, Gao CR, and Guo XJ
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- Autopsy, China, Forensic Sciences, Artificial Intelligence, Forensic Medicine
- Abstract
Objectives: To analyze the literature on artificial intelligence in forensic research from 2012 to 2022 in the Web of Science Core Collection Database, to explore research hotspots and developmental trends., Methods: A total of 736 articles on artificial intelligence in forensic medicine in the Web of Science Core Collection Database from 2012 to 2022 were visualized and analyzed through the literature measuring tool CiteSpace. The authors, institution, country (region), title, journal, keywords, cited references and other information of relevant literatures were analyzed., Results: A total of 736 articles published in 220 journals by 355 authors from 289 institutions in 69 countries (regions) were identified, with the number of articles published showing an increasing trend year by year. Among them, the United States had the highest number of publications and China ranked the second. Academy of Forensic Science had the highest number of publications among the institutions. Forensic Science International , Journal of Forensic Sciences , International Journal of Legal Medicine ranked high in publication and citation frequency. Through the analysis of keywords, it was found that the research hotspots of artificial intelligence in the forensic field mainly focused on the use of artificial intelligence technology for sex and age estimation, cause of death analysis, postmortem interval estimation, individual identification and so on., Conclusions: It is necessary to pay attention to international and institutional cooperation and to strengthen the cross-disciplinary research. Exploring the combination of advanced artificial intelligence technologies with forensic research will be a hotspot and direction for future research.
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- 2024
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13. Exploration and Practice of the "One Combination, Two Highlights, Three Combinations, Four in One" Innovative Talents Training Mode in Forensic Medicine.
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Yan JW, Sun JH, Wang HX, Wei ZW, Guo XJ, Li J, Gao CR, Zhang GQ, Liang XH, Zhang QQ, Wang HW, Li SJ, Wang YY, and Yun KM
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- Humans, Aptitude, Forensic Medicine education
- Abstract
Talent is one of the basic and strategic supports for building a modern socialist country in all aspects. Since the 1980s, the establishment of forensic medicine major and the cultivation of innovative talents in forensic medicine have become hot topics in higher education in forensic medicine. Over the past 43 years, the forensic medicine team of Shanxi Medical University has adhered to the joint education of public security and colleges, and made collaborative innovation, forming a training mode of "One Combination, Two Highlights, Three Combinations, Four in One" for innovative talents in forensic medicine. It has carried out "5+3/X" integrated reform, and formed a relatively complete talent training innovation mode and management system in teaching, scientific research, identification, major, discipline, team, platform and cultural construction. It has made a historic contribution to China's higher forensic education, accumulated valuable experience for the construction of first-class major and first-class discipline of forensic medicine, and provided strong support for the construction of the national new forensic talent training system. The popularization of this training mode is conducive to the rapid and sustainable development of forensic science, and provides more excellent forensic talents for national building, regional social development and the discipline construction of forensic science.
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- 2023
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14. Gene Expression Profiles at Different Time Points after Acute Myocardial Infarction in Mice.
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Li H, Jia X, Bai YQ, Wu P, Guo HL, Yun KM, Gao CR, and Guo XJ
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- Animals, Gene Expression Profiling methods, Mice, Mitogen-Activated Protein Kinases genetics, Mitogen-Activated Protein Kinases metabolism, RNA, Messenger, Ryanodine Receptor Calcium Release Channel genetics, Ryanodine Receptor Calcium Release Channel metabolism, Transcriptome, Computational Biology methods, Myocardial Infarction genetics, Myocardial Infarction metabolism
- Abstract
Objectives: To explore the mRNA differential expressions and the sequential change pattern in acute myocardial infarction (AMI) mice., Methods: The AMI mice relevant dataset GSE4648 was downloaded from Gene Expression Omnibus (GEO). In the dataset, 6 left ventricular myocardial tissue samples were selected at 0.25, 1, 4, 12, 24 and 48 h after operation in AMI group and sham control group, and 6 left ventricular myocardial tissue samples were selected in blank control group, a total of 78 samples were analyzed. Differentially expressed genes (DEGs) were analyzed by R/Bioconductor package limma, functional pathway enrichment analysis was performed by clusterProfiler, protein-protein interaction (PPI) network was constructed by STRING database and Cytoscape software, the key genes were identified by Degree topological algorithm, cluster sequential changes on DEGs were analyzed by Mfuzz., Results: A total of 1 320 DEGs were associated with the development of AMI. Functional enrichment results included cellular catabolic process, regulation of inflammatory response, development of muscle system and vasculature system, cell adhesion and signaling pathways mainly enriched in mitogen-activated protein kinase (MAPK) signaling pathway. The key genes of AMI included MYL7 , TSC22D2 , HSPA1A , BTG2 , NR4A1 , RYR2 were up-regulated or down-regulated at 0.25-48 h after the occurrence of AMI., Conclusions: The functional signaling pathway of DEGs and the sequential expression of key genes in AMI may provide a reference for the forensic identification of AMI.
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- 2022
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15. Mast cell tryptase and carboxypeptidase A expression in body fluid and gastrointestinal tract associated with drug-related fatal anaphylaxis.
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Guo XJ, Wang YY, Zhang HY, Jin QQ, and Gao CR
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- Anaphylaxis chemically induced, Anaphylaxis mortality, Anaphylaxis pathology, Autopsy, Biomarkers analysis, Carboxypeptidases A blood, Case-Control Studies, Drug Hypersensitivity mortality, Drug Hypersensitivity pathology, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Humans, Microscopy, Fluorescence, Predictive Value of Tests, Tryptases blood, Anaphylaxis enzymology, Carboxypeptidases A analysis, Drug Hypersensitivity enzymology, Pericardial Fluid enzymology, Tryptases analysis
- Abstract
Aim: To investigate the expression of mast cell tryptase and carboxypeptidase A in drug-related fatal anaphylaxis., Methods: The expression of mast cell tryptase and carboxypeptidase A in 15 autopsy cases of drug-related fatal anaphylaxis and 20 normal autopsy cases were detected. First, the expression of mast cell tryptase was determined in stomach, jejunum, lung, heart, and larynx by immunofluorescence. Different tissues were removed and fixed in paraformaldehyde solution, then paraffin sections were prepared for immunofluorescence. Using specific mast cell tryptase and carboxypeptidase A antibodies, the expression of tryptase and carboxypeptidase A in gastroenterology tract and other tissues were observed using fluorescent microscopy. The postmortem serum and pericardial fluid were collected from drug-related fatal anaphylaxis and normal autopsy cases. The level of mast cell tryptase and carboxypeptidase A in postmortem serum and pericardial fluid were measured using fluor enzyme linked immunosorbent assay (FEIA) and enzyme linked immunosorbent assay (ELISA) assay. The expression of mast cell tryptase and carboxypeptidase A was analyzed in drug-related fatal anaphylaxis cases and compared to normal autopsy cases., Results: The expression of carboxypeptidase A was less in the gastroenterology tract and other tissues from anaphylaxis-related death cadavers than normal controls. Immunofluorescence revealed that tryptase expression was significantly increased in multiple organs, especially the gastrointestinal tract, from anaphylaxis-related death cadavers compared to normal autopsy cases (46.67 ± 11.11 vs 4.88 ± 1.56 in stomach, 48.89 ± 11.02 vs 5.21 ± 1.34 in jejunum, 33.72 ± 5.76 vs 1.30 ± 1.02 in lung, 40.08 ± 7.56 vs 1.67 ± 1.03 in larynx, 7.11 ± 5.67 vs 1.10 ± 0.77 in heart, P < 0.05). Tryptase levels, as measured with FEIA, were significantly increased in both sera (43.50 ± 0.48 μg/L vs 5.40 ± 0.36 μg/L, P < 0.05) and pericardial fluid (28.64 ± 0.32 μg/L vs 4.60 ± 0.48 μg/L, P < 0.05) from the anaphylaxis group in comparison with the control group. As measured by ELISA, the concentration of carboxypeptidase A was also increased more than 2-fold in the anaphylaxis group compared to control (8.99 ± 3.91 ng/mL vs 3.25 ± 2.30 ng/mL in serum, 4.34 ± 2.41 ng/mL vs 1.43 ± 0.58 ng/mL in pericardial fluid, P < 0.05)., Conclusion: Detection of both mast cell tryptase and carboxypeptidase A could improve the forensic identification of drug-related fatal anaphylaxis.
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- 2015
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16. [Analysis of 105 Incarcerated Inmate's Death].
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Li YT, Song LJ, Cao AK, Zhou J, and Gao CR
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- Adolescent, Adult, Aged, Cardiovascular Diseases mortality, Female, Humans, Male, Middle Aged, Prisons, Respiratory Tract Diseases mortality, Suicide, Young Adult, Cause of Death, Prisoners statistics & numerical data
- Abstract
Objective: To analyze the characteristics in the incarcerated inmate's death, investigate the main cause of death of the incarcerated inmate and provide some information for forensic investigation., Methods: The cases from the forensic medical center of Shanxi Medical University from 2005 to 2013 were selected. The statistical analysis was performed by using the incarcerated inmate's gender, age, cause of death, manner of death, and disease as the markers., Results: There were 100 men, 5 women in the 105 incarcerated inmates; the age range was from 16 to 65 years; Inmates were mostly died of natural diseases, mainly in the respiratory and cardiovascular diseases; the main unnatural death was suicide with a rate of 54.5%., Conclusion: At present, most incarcerated inmate's death are due to natural diseases. The prison should improve incarcerated inmate's lives, work and health care conditions, and strengthen supervision of law enforcement.
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- 2015
17. [Diagnostic Significance of BAT in Anaphylaxis to Non-ionic Contrast Media].
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Zhang HY, Xu SJ, Tang XX, Niu JJ, Guo XJ, and Gao CR
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- Carboxypeptidases A metabolism, Complement Membrane Attack Complex metabolism, Flow Cytometry, Granulocytes cytology, Humans, Mast Cells cytology, Tetraspanin 30 metabolism, Anaphylaxis diagnosis, Basophils cytology, Contrast Media
- Abstract
Objective: To investigate the diagnostic significance of basophil activation test (BAT) in anaphylaxis to non-ionic contrast media through testing the content of CD63, mast cell-carboxypeptidase A3 (MC-CPA3), and terminal complement complex SC5b-9 of the individuals by testing their levels in the normal immune group and the anaphylaxis groups to β-lactam drugs and non -ionic contrast media., Methods: The CD63 expression of basophilic granulocyte in blood was detected by flow cytometry. The levels of MC-CPA3 in blood serum and SC5b-9 in blood plasma were detected by ELISA., Results: The CD63 expression of basophilic granulocyte in blood, the levels of MC-CPA3 and SC5b-9 of anaphylaxis to non-ionic contrast media and β-lactam drugs were significantly higher than that in normal immune group (P < 0.05)., Conclusion: There is activation of basophilic granulocytes, mast cells and complement system in anaphylaxis to non-ionic contrast media. BAT can be used to diagnose the anaphylaxis to non-ionic contrast media.
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- 2015
18. Relative Expression of Indicators for Wound Age Estimation in Forensic Pathology.
- Author
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Du QX, Wang XW, Zhang L, Li SQ, Gao CR, Wang YY, and Sun JH
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- Animals, Forensic Pathology, Intercellular Adhesion Molecule-1, Muscle, Skeletal metabolism, NF-kappa B, Proteins, RNA, Messenger genetics, RNA, Messenger metabolism, Rats, Rats, Sprague-Dawley, Real-Time Polymerase Chain Reaction, Regression Analysis, Reproducibility of Results, Time Factors, Contusions genetics, Gene Expression Profiling, Muscle, Skeletal injuries, Wound Healing genetics
- Abstract
Objective: In order to understand which kind of function genes play an important role for estimating wound age, the variation of difference genes' mRNA expression were compared after injury., Methods: The mRNA expression levels of seven candidate genes (ICAM-1, NF-κB, MX2, MT1, MT2, sTnI, and Cox6c) were analyzed in contused rat skeletal muscle at different time points using real-time fluorescent quantitative PCR (RT-qPCR). The raw Ct values were normalized relative to that of RPL32 mRNA, and converted to standard Ct values. At each time point after injury, the standard deviations (SD) of the standard Ct values were calculated by SPSS., Results: The expression trends of the seven genes were all found to be related to wound age, but there were lower variation coefficients and greater reliability of s TnI and Cox6c when compared with other genes., Conclusion: The genes encoding structural proteins or proteins that perform basic functions can be suitable for wound age estimation.
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- 2015
19. [Basophil CD63 expression in the blood of the anaphylactic shock rat].
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Jing LX, Guo XJ, Jin QQ, Huang MM, and Gao CR
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- Anaphylaxis blood, Anaphylaxis metabolism, Animals, Basophil Degranulation Test methods, Basophils metabolism, Biomarkers analysis, Disease Models, Animal, Female, Flow Cytometry, Lung pathology, Male, Ovalbumin administration & dosage, Phosphoric Diester Hydrolases blood, Phosphoric Diester Hydrolases immunology, Pyrophosphatases immunology, Random Allocation, Rats, Rats, Wistar, Tetraspanin 30 metabolism, Anaphylaxis diagnosis, Basophils immunology, Lung metabolism, Pyrophosphatases blood, Tetraspanin 30 blood
- Abstract
Objective: To explore the value of flow cytometry in anaphylactic shock diagnosis by CD63 expression being detected using flow cytometry to conform the activation of basophils., Methods: Sixteen rats were randomly divided into two groups: control group and anaphylactic shock group. The model of anaphylactic shock rat with ovalbumin injection was established. CD63, CD45 and CD203c antibody combination, flow cytometry was employed to detected blood basophil CD63 expression. Immunofluorescence method was employed to observe the CD63 immunofluorescence staining in the rat lung tissue., Results: (1) Pure basophils were obtained by CD45 and CD203c gating. (2) The percentages of basophils CD63 were (17.34 +/- 2.04)% and (1.52 +/- 0.35)% in the experimental and control group, respectively. The differences between two groups were statistically significant (P < 0.01). (3) Compared with the control group, the expression of CD63 in basophils increased in anaphylactic shock lung tissue., Conclusion: The detection of CD63 by flow cytometry could be the supplement of vivo allergic reactions and have good clinical value.
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- 2012
20. [Changes of prostaglandin D2,carboxypeptidase A3 and platelet activating factor in guinea pig in anaphylactic shock].
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Yang K, Guo XJ, Yan XB, and Gao CR
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- 1-Alkyl-2-acetylglycerophosphocholine Esterase administration & dosage, 1-Alkyl-2-acetylglycerophosphocholine Esterase pharmacology, Anaphylaxis blood, Anaphylaxis prevention & control, Animals, Brain pathology, Case-Control Studies, Disease Models, Animal, Egg Proteins administration & dosage, Enzyme-Linked Immunosorbent Assay, Female, Guinea Pigs, Leukotriene E4 urine, Male, Mice, Platelet Activating Factor drug effects, Time Factors, Anaphylaxis diagnosis, Brain metabolism, Carboxypeptidases blood, Platelet Activating Factor metabolism, Prostaglandin D2 blood
- Abstract
Objective: To detect the changes of leukotriene E4(LTE4), prostaglandin D2(PGD2), carboxypeptidase A3(CPA3) and platelet activating factor (PAF) in guinea pigs died from anaphylactic shock., Methods: Guinea pigs were used for establishing anaphylactic shock models. The levels of LTE4, PGD2 and CPA3, and PAF were detected in urine, plasma, and brain tissues with ELISA kit, respectively. The significant biomarkers were selected comparing with control group. The changes of PGD2, CPA3 and PAF in the guinea pigs at time zero, 12 and 24 hours after death were observed and compared respectively. The effect of platelet activating factor acetylhydrolase (PAF-AH) to PAF in guinea pig brain was examined and compared., Results: There were no statistically differences of LTE4 levels in urine observed between experimental group and control group. The levels of CPA3, PGD2 and PAF in the experimental group were significantly higher than that in the control group at 0 h. The levels of PAF at 12 and 24 hours after anaphylactic shock were significantly higher than that in the control group. The levels of PAF decreased significantly after pretreatment with PAF-AH., Conclusion: LTE4 in urine cannot be selected as a biomarker to determine the anaphylactic shock. PGD2 and CPA3 in plasma, and PAF in brain tissue may be used as biomarkers to determine the anaphylactic shock. PAF-AH may be potentially useful for clinical treatment of anaphylactic shock.
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- 2012
21. [The expression of VCAM-1 in organs of rats died of anaphylactic shock].
- Author
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Li XJ, Guo XJ, and Gao CR
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- Anaphylaxis chemically induced, Anaphylaxis immunology, Anaphylaxis pathology, Animals, Antibodies, Monoclonal therapeutic use, Brain metabolism, Disease Models, Animal, Female, Immunohistochemistry, Kidney metabolism, Male, Random Allocation, Rats, Rats, Sprague-Dawley, Time Factors, Vascular Cell Adhesion Molecule-1 immunology, Anaphylaxis metabolism, Lung metabolism, Vascular Cell Adhesion Molecule-1 metabolism
- Abstract
Objective: To investigate the effect and expression of the vascular cell adhesion molecule-1 (VCAM-1) in organs of rats died of anaphylactic shock., Methods: The models of anaphylactic shock in rats were made and the immunohistochemistry of SABC was used to detect as follows: (1) The expression of VCAM-1 in rat lung, heart, brain, kidney, liver, spleen, stomach and intestine. (2) VCAM-1 levels in lungs at 10 min, 30 min after the allergic shock, and the time of death. (3) VCAM-1 levels in lungs of rats after the intervention of anti-VCAM-1., Results: After the death, the expression VCAM-1 in lungs increased significantly relative to the control group and followed the extension of shock. In the rats which were injected with the anti-VCAM-1, the expression of VCAM-1 in lungs reduced., Conclusion: (1) The expression of VCAM-1 shows difference in the various organs of rats after anaphylactic shock. The change of VCAM-1 is the most obvious in lungs and would increase followed the extension of anaphylactic shock. (2) After the anaphylactic shock, anti-VCAM-1 can inhibit the expression of VCAM-1 in rat lung.
- Published
- 2011
22. [Changes of ICAM-1 mRNA expression in rats' skeletal muscle after contusion].
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Pei M, Sun JH, and Gao CR
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- Animals, Contusions pathology, Female, Forensic Pathology, In Situ Hybridization, Fluorescence, Intercellular Adhesion Molecule-1 genetics, Male, RNA, Messenger genetics, RNA, Messenger metabolism, Random Allocation, Rats, Rats, Sprague-Dawley, Time Factors, Wound Healing, Contusions metabolism, Hindlimb injuries, Intercellular Adhesion Molecule-1 metabolism, Muscle, Skeletal injuries, Muscle, Skeletal metabolism
- Abstract
Objective: To investigate the expression of intercellular adhesion molecular-1 (ICAM-1) mRNA in contused skeletal muscle of rats using fluorescence in situ hybridization (FISH) and its relationship with the contusion interval., Methods: To make the contusion models with rats skeletal muscle. The samples were taken to extract mRNA at 0.5 h, 1 h, 6 h, 12 h, 18 h, 24 h, 30 h and from the control group after contusion respectively. FISH was performed on frozen section samples and the sections were observed using LSCM., Results: The expression of ICAM-1 mRNA peaked at 6 h in skeletal muscle after contusion. Its level fell to 3.46 times the level of control group at 18 h and then increased again., Conclusion: The time-order expression of ICAM-1 mRNA in 30 hours after contusion is potentially useful for estimation of early wound age.
- Published
- 2009
23. [Expression of tryptase and chymase in human lung tissue of anaphylactic shock].
- Author
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Wei ZY, Gao CR, and Pei M
- Subjects
- Adolescent, Adult, Anaphylaxis pathology, Cadaver, Carbon Monoxide Poisoning enzymology, Carbon Monoxide Poisoning pathology, Child, Child, Preschool, Female, Fluoroimmunoassay methods, Forensic Pathology, Humans, Infant, Lung pathology, Male, Middle Aged, Staining and Labeling, Young Adult, Anaphylaxis enzymology, Chymases metabolism, Lung enzymology, Mast Cells enzymology, Tryptases metabolism
- Abstract
Objective: To explore the expression of tryptase and chymase in human lung tissue of anaphylactic shock and its value for forensic medicine., Methods: With ten carbon monoxide poisoning cases as control group, the levels of tryptase and chymase were observed by immunofluorescence and analyzed using the Image Analyze and the Image-pro plus 5.0.2. The positive mast cells were counted and the levels of the tryptase and chymase were calculated respectively., Results: There was a statistically significant difference (P < 0.05) for the tryptase and chymase concentrations in the lung tissue between the anaphylactic shock group and the control group., Conclusion: The levels of the tryptase and the chymase expression are greatly increased in human lung tissue of anaphylactic shock, which may provide the morphological evidence and reference for the diagnosis of anaphylactic shock in forensic practice.
- Published
- 2009
24. [Present study on the forensic medicine diagnosis of the sudden erethistic death].
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Gao CR, Xue SH, and Wang YY
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- Anaphylaxis metabolism, Anaphylaxis pathology, Forensic Pathology, Gastric Mucosa metabolism, Histamine blood, Histamine metabolism, Humans, Immunoglobulin E metabolism, Immunohistochemistry, Intestinal Mucosa metabolism, Mast Cells enzymology, Retrospective Studies, Substance P blood, Trypsin metabolism, Anaphylaxis blood, Death, Sudden, Immunoglobulin E blood, Substance P metabolism, Trypsin blood
- Abstract
The death caused of anaphylactic shock is common in clinical medicine and medicolegal expertise, but it is a nodus to diagnose sudden death from allergy. In recent years, to provide objective and precise morphological evidence and index of diagnosis for sudden death from allergy, scholars of internal and overseas studied the content of IgE, HT, mast cell tryptase and SP in the serum of the death died of anaphylactic shock, and their immune express in lung and stomach intestine. In this text we reviewed the present study and existing problems of the forensic medicine diagnosis of the sudden erethistic death.
- Published
- 2006
25. [Expression of substance P in human laryngopharynx and gastrointestine in sudden erethistic death].
- Author
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Sun HW, Gao CR, Ren GM, Sun JH, Lu J, and Wang YY
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- Adolescent, Adult, Anaphylaxis pathology, Autopsy, Child, Death, Sudden, Cardiac pathology, Female, Humans, Immunohistochemistry, Intestinal Mucosa metabolism, Male, Middle Aged, Mucous Membrane metabolism, Young Adult, Anaphylaxis metabolism, Death, Sudden, Gastric Mucosa metabolism, Pharynx metabolism, Substance P metabolism
- Abstract
Objective: To study the expression of substance P (SP) in human sudden erethistic death, and to seek objective morphological supports to diagnose sudden erethistic death for forensic medicine., Methods: The expression of SP was detected with immunohistochemical technique on 15 human laryngopharynx and gastrointestine of sudden erethistic death, and 20 sudden death of heart attack as control. The images of SP were analyzed by image analyzer, and the positive indexes (PI) were calculated., Results: SP expression in the experimental groups was significantly stronger than that in the control one (P < 0.001)., Conclusion: SP expression can offer an objective morphological reference support for forensic diagnosing sudden erethistic death.
- Published
- 2006
26. [Lethal anaphylactic shock model induced by human mixed serum in guinea pigs].
- Author
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Ren GM, Bai JW, and Gao CR
- Subjects
- Anaphylaxis blood, Animals, Death, Sudden, Female, Forensic Medicine, Guinea Pigs, Humans, Male, Radioimmunoassay, Random Allocation, Allergens, Anaphylaxis immunology, Disease Models, Animal, Immunoglobulin G blood, Serum immunology
- Abstract
Objective: To establish an anaphylactic shock model induced by human mixed serum in guinea pigs., Methods: Eighteen guinea pigs were divided into two groups: sensitized and control, The sensitized group were immunized intracutaneously with human mixed serum and then induced by endocardiac injection after 3 weeks., Results: Symptoms of anaphylactic shock appeared in the sensitized group. The level of serum IgE were increased in the sensitized group significantly., Conclusions: An animal model of anaphylactic shock wer established successfully. It provide a tool for both forensic study and anaphylactic shock therapy.
- Published
- 2005
27. [The application of telomere DNA in age estimation of forensic medicine].
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Sun HW and Gao CR
- Subjects
- Adolescent, Adult, Blotting, Southern methods, Cell Division physiology, Humans, Polymorphism, Restriction Fragment Length, Telomere genetics, Aging physiology, DNA analysis, Forensic Medicine methods, Telomere physiology
- Abstract
Estimating tooth age and skeletal age are the two primary methods in age estimation of forensic medicine. But they are often impacted with geographical environment, nutrition, habitation and ethenologic differences, so the accuracy will be reduced, especially to the adult. With the study of telomere, it is certain that the length of the telomere DNA can reflect the cell division and represent the cell lifespan, and it has some pertinence to the age of the donor, so to measure the length of telomere DNA is a new and valuable method for age estimation in the forensic medicine.
- Published
- 2005
Catalog
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