139 results on '"Handan Yavuz"'
Search Results
2. Quartz crystal microbalance based histidine sensor
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Merve Sönmezler, Erdoğan Özgür, Handan Yavuz, and Adil Denizli
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l-histidine ,amino acid detection ,molecular imprinting ,quartz crystal microbalance ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Herein, quartz crystal microbalance (QCM) biosensor is prepared for the detection of l-histidine by attachment of l-histidine imprinted poly(EGDMA-MAH/Cu(II)) nanoparticles on QCM electrode. The imprinted nanoparticles with the size of 86.43 nm were synthesized via miniemulsion polymerization reaction. Prepared QCM sensors were characterized with ellipsometer, contact angle measurements and FTIR. The thickness measurements demonstrated that the particle thin films were almost monolayer. l-histidine solutions with a concentration range between 6.44 μM and 225.6 μM were introduced to QCM system to determine the adsorption kinetics. Selectivity of the l-histidine imprinted nanoparticles were examined using d-histidine and l-tryptophan as competitor molecules. l-histidine imprinted QCM biosensors was also used for RNAase, lysozyme, cytochrome-C and BSA to investigate the competitive adsorption of surface histidine exposed proteins.
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- 2019
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3. Prism coupler-based sensor system for simultaneous screening of synthetic glucocorticosteroid as doping control agent
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Sari, Esma, Üzek, Recep, Duman, Memed, Alagöz, Handan Yavuz, and Denizli, Adil
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- 2018
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4. Development of Gold Nanoparticles Decorated Molecularly Imprinted–Based Plasmonic Sensor for the Detection of Aflatoxin M1 in Milk Samples
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Semra Akgönüllü, Handan Yavuz, and Adil Denizli
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aflatoxin M1 ,mycotoxins ,gold nanoparticles ,plasmonic sensor ,enhanced surface plasmon resonance ,molecularly imprinted polymers ,Biochemistry ,QD415-436 - Abstract
Aflatoxins are a group of extremely toxic and carcinogenic substances generated by the mold of the genus Aspergillus that contaminate agricultural products. When dairy cows ingest aflatoxin B1 (AFB1)−contaminated feeds, it is metabolized and transformed in the liver into a carcinogenic major form of aflatoxin M1 (AFM1), which is eliminated through the milk. The detection of AFM1 in milk is very important to be able to guarantee food safety and quality. In recent years, sensors have emerged as a quick, low–cost, and reliable platform for the detection of aflatoxins. Plasmonic sensors with molecularly imprinted polymers (MIPs) can be interesting alternatives for the determination of AFM1. In this work, we designed a molecularly–imprinted–based plasmonic sensor to directly detect lower amounts of AFM1 in raw milk samples. For this purpose, we prepared gold–nanoparticle–(AuNP)−integrated polymer nanofilm on a gold plasmonic sensor chip coated with allyl mercaptan. N−methacryloyl−l−phenylalanine (MAPA) was chosen as a functional monomer. The MIP nanofilm was prepared using the light–initiated polymerization of MAPA and ethylene glycol dimethacrylate in the presence of AFM1 as a template molecule. The developed method enabled the detection of AFM1 with a detection limit of 0.4 pg/mL and demonstrated good linearity (0.0003 ng/mL–20.0 ng/mL) under optimized experimental conditions. The AFM1 determination was performed in random dairy farmer milk samples. Using the analogous mycotoxins, it was also demonstrated that the plasmonic sensor platforms were specific to the detection of AFM1.
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- 2021
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5. Molecularly Imprinted Polymers-based Biosensors for Clinical Applications
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Yeşeren Saylan, Handan Yavuz, and Adil Denizli
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- 2022
6. Plasmonic Sensors for Detection of Chemical and Biological Warfare Agents
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Handan Yavuz, Yeşeren Saylan, Nilay Bereli, Semra Akgönüllü, Deniz Türkmen, and Adil Denizli
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Chemical Warfare Agents ,Chemistry ,Biological warfare ,Homeland security ,Nanotechnology - Published
- 2021
7. Molecularly Imprinted Polymer Based Sensors for Medical Applications.
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Yeseren Saylan, Semra Akgönüllü, Handan Yavuz, Serhat ünal, and Adil Denizli
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- 2019
- Full Text
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8. Synthesis of molecularly imprinted magnetic nanoparticles for selective cytidine adsorption
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Handan Yavuz, Semra Akgönüllü, Adil Denizli, and Emine Öngün
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chemistry.chemical_compound ,Adsorption ,Chemistry ,Molecularly imprinted polymer ,Magnetic nanoparticles ,Cytidine ,Combinatorial chemistry - Published
- 2020
9. Molecular Imprinting Technology for Biomimetic Assemblies
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Sevgi Aslıyüce, Semra Akgönüllü, Ilgım Göktürk, Adil Denizli, Deniz Türkmen, Nilay Bereli, Duygu Çimen, and Handan Yavuz
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Molecular imprinting,biomimetic,separation,sensing,polymer brushes,artificial membranes,drug delivery,tissue engineering ,Engineering ,Chemistry ,Mühendislik ,Nanotechnology ,Molecular imprinting - Abstract
The term biomimetic can be simply defined as the examination of nature. The scientists inspired by the enormous diversity of nature to solve human problems or facilitate daily life by mimicking natural models, systems, and elements especially in the biomedical and therapeutic applications to make better drugs, artificial organs, sensing instruments, etc. Biological recognition elements like proteins, antibodies, enzymes, DNA, lectins, aptamers, cells, and viruses have been heavily used to ensure specificity in such applications in spite of their lack of stability and reusability. However, in the last two decades molecularly imprinted polymers, MIPs, have been synthesized as an alternative to mimic natural biological interactions for a broad spectrum of templates by means of coordinating functional monomers around template in the presence of cross-linker. This review will outline the broad contours of biomimetics prepared by molecular imprinting techniques and their practical applications in the separation techniques, tissue engineering applications, biomimetic surfaces, sensors, artificial membranes, and drug delivery systems.
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- 2020
10. Protein chromatography by molecular imprinted cryogels
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Adil Denizli, Handan Yavuz, and Nilay Bereli
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chemistry.chemical_classification ,Chromatography ,010405 organic chemistry ,Chemistry ,010401 analytical chemistry ,Clinical Biochemistry ,Pharmaceutical Science ,Polymer ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,Analytical Chemistry ,Protein recognition ,Protein chromatography ,Molecular imprinting - Abstract
Molecularly imprinted cryogels for protein recognition have received much attention in recent years with the development of supermacroporous polymer systems. Molecularly imprinted cryogels, which o...
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- 2020
11. Electrochromatographic separation of hydrophobic amino acid enantiomers by molecularly imprinted capillary columns
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Fatma Yılmaz, Adil Denizli, Ilgım Göktürk, Suleyman Asir, Koray Şarkaya, and Handan Yavuz
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0106 biological sciences ,chemistry.chemical_classification ,0303 health sciences ,Capillary electrochromatography ,Chromatography ,Capillary action ,Tryptophan ,Bioengineering ,Phenylalanine ,01 natural sciences ,Applied Microbiology and Biotechnology ,Biochemistry ,Amino acid ,03 medical and health sciences ,chemistry.chemical_compound ,Monomer ,chemistry ,Polymerization ,010608 biotechnology ,Enantiomer ,030304 developmental biology - Abstract
In the present study, the enantiomeric forms of hydrophobic amino acids ( l -tryptophan, l -tyrosine, and l -phenylalanine) were separated by molecularly imprinted capillary columns (MICC) via Capillary Electrochromatography (CEC) for the first time. The monomer ratio, crosslinker ratio, template molecule ratio, the porogen ratio and type, polymerization time, and also the effect of temperature were examined to increase the permeability properties of MICC. FTIR, SEM and BET analyses were realized for the characterization of MICC. The effect of the electric field, organic solvent ratio, and pressure were carried out experimentally to determine the optimum conditions. The separation performances of MICC and the non-imprinted capillary columns (NICC) were compared electrochromatographically.
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- 2020
12. Separation of histidine enantiomers by capillary electrochromatography with molecularly imprinted monolithic columns
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Koray Şarkaya, Sisem Ektirici, Ilgım Göktürk, Handan Yavuz, Adil Denizli, Suleyman Asir, and Fatma Yılmaz
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Capillary electrochromatography ,Chromatography ,Chemistry ,Enantiomer ,Molecular imprinting ,Histidine - Published
- 2020
13. Extracorporeal affinity systems and immunoadsorption therapies
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Handan Yavuz, Adil Denizli, and Nilay Bereli
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Chemistry ,Pharmacology ,Immunoadsorption ,Extracorporeal - Published
- 2022
14. List of contributors
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Hayder A. Abdulbari, Dalia Samir Ahmed Mahdy, Semra Akgönüllü, Fahima Akther, Mohammad Feroz Alam, Ibrahim M. Alarifi, Sidra Amir, Tatiana Avsievich, Nilay Bereli, Muhammad Bilal, Alexander Bykov, Santhosh Chidangil, Adil Denizli, Katja Ferenz, Sowmya Hari, Sumreen Hayat, Dzuliana Fatin Jamil, Ozan Karaman, Mohsin Khurshid, Jijo Lukose, Christian Mayer, Setti Sudharsan Meenambiga, Igor Meglinski, Ganesh Mohan, Saima Muzammil, Nam-Trung Nguyen, Yoong Sheng Phang, Alexey Popov, Khaliqur Rahman, Rozaini Roslan, Punniavan Sakthiselvan, Yeşeren Saylan, Shah Bahrullah Shah, Aqsa Shahid, Shamee Shastry, Hang T. Ta, Ahmet Fatih Tabak, Huong D.N. Tran, Salah Uddin, Devasena Umai, Zhi Ping Xu, Sunishtha S. Yadav, Yanjun Yang, Humaira Yasmeen, Handan Yavuz, Maryam Zain, Jun Zhang, Yiping Zhao, Ruixue Zhu, and Aimen Zulfiqar
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- 2022
15. List of contributors
- Author
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Vojtech Adam, Semra Akgönüllü, P. Aparna, Neha Arya, Amirmansoor Ashrafi, Onkar Awadhiya, Gautam Bacher, Nilay Bereli, Sunil Bhand, Debasis Biswas, Thomas Briese, Priya Chauhan, C. Chikkaputtaiah, Rashmi Chowdhary, Vishal Das, Akash Deep, H.P. Deka Boruah, Adil Denizli, Shreyas Deshpande, Shimaa Eissa, Sonu Gandhi, Mayank Garg, Amrita Ghosh, K. Yugender Goud, Vinod Singh Gour, Arushi Gupta, Divya Gupta, Amilan D. Jose, Joshy Joseph, Rajnish Joshi, Raju Khan, Ashok Kumar, Deepak Kumar, Subhasis Mahari, H.S. Mann, Adarsh Meher, Rupesh K. Mishra, Atripan Mukherjee, Soumajit Mukherjee, Divya Namdeo, Roger Narayan, Vanya Nayak, Rajeev Nema, Ravi Ranjan Kumar Niraj, Navin Pai, Mintu Pal, Smriti Panda, Annu Pandey, Arpana Parihar, Dipesh Singh Parihar, Gaurang Patel, Priyanka Pulugu, Nidhi Puranik, Rahim Rahimi, Bindu Rameshan, Pushpesh Ranjan, Lukas Richtera, Akanksha Roberts, Mohd. Abubakar Sadique, Saurabh Saigal, Yeşeren Saylan, Amit L. Sharma, Vinay Sharma, Anirudh K. Singh, Himadri Singh, Kshitij R.B. Singh, Ravindra Pratap Singh, Suman Singh, Ayushi Singhal, Lia Stanciu, Petra Strakova, M.K. Verma, Y.K. Verma, Akanksha Wakhare, Ashvini Kumar Yadav, Shalu Yadav, Handan Yavuz, Tabassum Zafar, and Amin Zareei
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- 2022
16. Scaling up of biosensors for clinical applications and commercialization
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Yeşeren Saylan, Semra Akgönüllü, Nilay Bereli, Handan Yavuz, and Adil Denizli
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- 2022
17. An Overview of Youtube Videos as An Information and Education Source For Idiopathic Ventricular Arrhythmia
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mer Baycan, Adem Atici, Mustafa su, Yusuf lmaz, Handan Yavuz, Salih m, Muhsin znaneci, and Mustafa kan
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General Medicine - Abstract
Objective: The Internet is a widespread and increasingly used resource for obtaining medical information by both patients and public health care providers. Our aim in this study was to evaluate the accuracy and quality of the information in idiopathic ventricular arrhythmia (IVA) videos posted on YouTube. Materials and Methods: "Idiopathic Ventricular Dysrhythmia" or "Idiopathic Ventricular Arrhythmia" keywords of YouTube search engine were queried. 48 videos suitable for 2-15 minutes were included in the study. The popularity of videos was judged by an index called the video strength index (VPI). The reliability of the videos was assessed with the DISCERN score. The quality of the information resource measured using the Global Quality Score (GQS). Results: The average duration of the videos was 444 ± 220 seconds, and the average number of views is 11241 ± 865, with a median view count of 1085 (151-22012). The median VPI value of the videos was 4 (0-34), the mean DISCERN score was 3.1 ± 0.5, and the mean GQS score was 3.6 ± 1.1. Conclusion: YouTube serves as an easily accessible and inexpensive data center for patient education and understanding the illness. However, the popular YouTube videos on IVA administration appear to be largely lacking in accuracy, reliability, and consistency, as they are vulnerable to unreliable information.
- Published
- 2023
18. Quartz crystal microbalance biosensor for label-free MDA MB 231 cancer cell detection via notch-4 receptor
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Monireh Bakhshpour, Handan Yavuz, Adil Denizli, and Ayse Kevser Piskin
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Breast Neoplasms ,Biosensing Techniques ,02 engineering and technology ,01 natural sciences ,Analytical Chemistry ,Malignant transformation ,Mice ,Breast cancer ,Limit of Detection ,Cell surface receptor ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Receptor, Notch4 ,Receptor ,Polyhydroxyethyl Methacrylate ,Chemistry ,010401 analytical chemistry ,Cancer ,Quartz crystal microbalance ,021001 nanoscience & nanotechnology ,medicine.disease ,0104 chemical sciences ,Cancer cell ,Quartz Crystal Microbalance Techniques ,Biophysics ,Nanoparticles ,0210 nano-technology ,Antibodies, Immobilized ,Biosensor - Abstract
Breast cancer is the most common cancer in women with increasing insidance. Breast cancer occurs as a result of some molecular changes, such as aberrant or dysregulated expression of receptors, in breast epithelial cells. Therefore, breast cancer cells can be detected through their membrane receptors using specific antibodies. This study aims to form a quartz crystal microbalance (QCM) biosensor to detect breast cancer cells. Notch receptor signaling directs many pathways in developing breast tissue and its expression is found to be aberrant or disregulated in metastatic breast cancer cells. Its involvement in malignant transformation makes it a potential drug target. The human metastatic breast cancer cells, MDA MB 231 cells, are used here as a model due to the overexpression of notch-4 receptor on their membranes. First, to increase the surface area of the chip poly(2-hydroxyethyl methacrylate-PHEMA) nanoparticles were synthesized and were placed on QCM chip surface. Then, the surface was further modified and functionalized by binding notch-4 receptor antibody using carbodiimide. Nanoparticle coated and antibody attached QCM chips were characterized via FTIR-ATR, ellipsometry, contact angle measurements and by atomic force microscopy. MDA MB 231 cell samples ranging in numbers between 50-300 cells/ml were introduced to the functionalized QCM chip at a flow rate of 1.0 mL/min and the resonance frequency (f0) was recorded. Then, cell samples were applied to the QCM biosensor and the resonance frequency was monitored. The binding mode fitted best to Langmuir isotherm model. Sensitivity is found to be high and the selectivity as tested by competitive adsorption of L929 mouse fibroblast cells showed that QCM biosensor was 17.5 times more selective for MDA MB 231 cells than the fibroblast cells. The chip was reusable and was stable over 3 months. These results indicate that, the notch-4 receptor antibody PHEMA nanoparticle QCM biosensor is highly selective and efficient system that may be used for cancer cell detection.
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- 2019
19. Preparation of Notch-4 Receptor Containing Quartz Crystal Microbalance Biosensor for MDA MB 231 Cancer Cell Detection
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Monireh, Bakhshpour, Ayse Kevser, Piskin, Handan, Yavuz, and Adil, Denizli
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Mice ,Limit of Detection ,Quartz Crystal Microbalance Techniques ,Animals ,Humans ,Proteins ,Breast Neoplasms ,Female ,Biosensing Techniques ,Quartz ,Receptor, Notch4 - Abstract
Quartz crystal microbalance (QCM) is a highly sensitive system that is used as a biosensor for biomolecules and cells. Detection and characterization of cancer cells in circulation or biopsy samples is of crucial importance for cancer diagnosis. Here, we introduce approaches for breast cancer cell detection via their surface molecules. The sensor system is based on preliminary coating of QCM chip with polymeric nanoparticles to increase the surface area and allow for the attachment of proteins to the chip surface. This is followed by the attachment of a specific protein in order to functionalize the chip. Breast cancer cells and fibroblast cells as control are cultured and applied to this chip. The functionalized QCM system can detect breast cancer cells with high affinity and selectivity. Here, we present the preparation methods of QCM-based sensors for selective detection of MDA MB 231 cancer cells. Selectivity of QCM-based sensor is carried out in the presence of L929 mouse fibroblast cells.
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- 2021
20. Preparation of Notch-4 Receptor Containing Quartz Crystal Microbalance Biosensor for MDA MB 231 Cancer Cell Detection
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Monireh Bakhshpour, Handan Yavuz, Ayse Kevser Piskin, and Adil Denizli
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chemistry.chemical_classification ,Chemistry ,Biomolecule ,Nanoparticle ,Cancer ,Quartz crystal microbalance ,medicine.disease ,medicine.anatomical_structure ,Cancer cell ,Biophysics ,medicine ,Selectivity ,Fibroblast ,Biosensor - Abstract
Quartz crystal microbalance (QCM) is a highly sensitive system that is used as a biosensor for biomolecules and cells. Detection and characterization of cancer cells in circulation or biopsy samples is of crucial importance for cancer diagnosis. Here, we introduce approaches for breast cancer cell detection via their surface molecules. The sensor system is based on preliminary coating of QCM chip with polymeric nanoparticles to increase the surface area and allow for the attachment of proteins to the chip surface. This is followed by the attachment of a specific protein in order to functionalize the chip. Breast cancer cells and fibroblast cells as control are cultured and applied to this chip. The functionalized QCM system can detect breast cancer cells with high affinity and selectivity. Here, we present the preparation methods of QCM-based sensors for selective detection of MDA MB 231 cancer cells. Selectivity of QCM-based sensor is carried out in the presence of L929 mouse fibroblast cells.
- Published
- 2021
21. Preparation of Surface Plasmon Resonance Aptasensor for Human Activated Protein C Sensing
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Semra, Akgönüllü, Seda, Koyun, Handan, Yavuz, Arzum, Erdem, and Adil, Denizli
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Limit of Detection ,Humans ,Biosensing Techniques ,DNA ,Aptamers, Nucleotide ,Surface Plasmon Resonance ,Protein C - Abstract
Nucleic acid aptamers are an emerging class of artificial ligands and have recently gained attention in several areas. Here we report the design of a surface plasmon resonance (SPR) aptasensor for highly sensitive and selective sensing of human activated protein C (APC). First, DNA aptamer (DNA-Apt) specific for APC is complexed with N-methacryloyl-L-cysteine (MAC) monomer. Then, 2-hydroxyethyl methacrylate (HEMA) and cyanamide are mixed with the DNA-Apt/MAC complex. The SPR aptasensor is characterized by atomic force microscopy, ellipsometry, and contact angle measurements. Selectivity of SPR aptasensor is carried out in the presence of myoglobin (Myb), hemoglobin (Hb), and bovine serum albumin (BSA). Limit of detection (LOD) and limit of quantification (LOQ) values are 1.5 ng mL
- Published
- 2021
22. Preparation of Surface Plasmon Resonance Aptasensor for Human Activated Protein C Sensing
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Arzum Erdem, Seda Koyun, Handan Yavuz, Adil Denizli, and Semra Akgönüllü
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ultraviolet radiation ,limit of quantitation ,Aptamer ,cyanamide ,education ,Biosensing Techniques ,protein C ,Contact angle ,chemistry.chemical_compound ,Aptasensors ,Limit of Detection ,Surface plasmon resonance ,bovine serum albumin ,Humans ,human ,Bovine serum albumin ,contact angle ,n methacryloyl cysteine ,Detection limit ,atomic force microscopy ,Chromatography ,biology ,activated protein C ,2 hydroxyethyl methacrylate ,aptamer ,DNA ,DNA aptamer ,hemoglobin ,monomer ,Aptamers, Nucleotide ,unclassified drug ,Monomer ,chemistry ,Myoglobin ,protein analysis ,myoglobin ,biology.protein ,Nucleic acid ,chemical modification ,genetic procedures ,ellipsometry - Abstract
Nucleic acid aptamers are an emerging class of artificial ligands and have recently gained attention in several areas. Here we report the design of a surface plasmon resonance (SPR) aptasensor for highly sensitive and selective sensing of human activated protein C (APC). First, DNA aptamer (DNA-Apt) specific for APC is complexed with N-methacryloyl-L-cysteine (MAC) monomer. Then, 2-hydroxyethyl methacrylate (HEMA) and cyanamide are mixed with the DNA-Apt/MAC complex. The SPR aptasensor is characterized by atomic force microscopy, ellipsometry, and contact angle measurements. Selectivity of SPR aptasensor is carried out in the presence of myoglobin (Myb), hemoglobin (Hb), and bovine serum albumin (BSA). Limit of detection (LOD) and limit of quantification (LOQ) values are 1.5 ng mL?1 and 5.2 ng mL?1, respectively. DNA-Apt SPR aptasensor performance for APC detection is also examined in artificial plasma. © 2022, Springer Science+Business Media, LLC, part of Springer Nature.
- Published
- 2021
23. Molecular Imprinting of Macromolecules for Sensor Applications.
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Yeseren Saylan, Fatma Yilmaz, Erdogan özgür, Ali Derazshamshir, Handan Yavuz, and Adil Denizli
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- 2017
- Full Text
- View/download PDF
24. Magnetic Nanoparticles and Their Biomedical Applications
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Tahira Qureshi, Fatma Denizli, Kemal Çetin, Adil Denizli, Handan Yavuz, and Deniz Türkmen
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chemistry.chemical_classification ,Materials science ,Magnetism ,Biomolecule ,Mühendislik ,food and beverages ,Nanoparticle ,Nanotechnology ,Biomedical applications,bioseparation,drug delivery,hyperthermia,magnetic nanoparticle ,Polymer ,equipment and supplies ,Functional diversity ,Engineering ,chemistry ,Drug delivery ,Magnetofection ,Magnetic nanoparticles ,human activities - Abstract
The combination of magnetism and nanotechnology has presented promising materials: magnetic nanoparticles. These materials have been getting more attention due to their “size‐dependent functionality”. There is a critical size for nanoparticles that their properties change. Materials with various functions can be synthesized with the desired properties since a wide range of polymers including natural and synthetic polymers can be utilized in the production of the magnetic nanoparticles. Furthermore, they can be more selective and specific with the conjugation target-specific ligands. This structural and functional diversity enables these materials to be used in a wide range of areas. In this review, we discuss the main components of the magnetic nanoparticles and their examples in biomedical applications. They can be used as contrast agents in magnetic resonance imaging; delivery systems in the controlled release of therapeutic agents; supporting materials for separation, isolation, and purification of biomolecules. They can be also functioned in hyperthermia and magnetofection for gene therapy. However, even though their increasing research interest, magnetic nanoparticles still need to be improved to be more popular in the commercial area. We hope that these functional materials will present promising possibilities in nanotechnology and biomedicine in near future.
- Published
- 2019
25. Oral Chelation Therapy for Cadmium Poisoning with Cd(II)-Mac Imprinted pHEMAC Nanoparticles
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Handan Yavuz, Adil Denizli, Mitra Jalilzadeh, and Duygu Çimen
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Langmuir ,Adsorption ,Metal poisoning ,Chemistry ,Nanoparticle ,chemistry.chemical_element ,Freundlich equation ,Zinc ,Selectivity ,Copper ,Nuclear chemistry - Abstract
In this study was prepared Cd(II)) ion-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-cysteine methylester) pHEMAC nanoparticles for the oral treatment of heavy metal poisoning. Cd(II) ion-imprinted pHEMAC nanoparticles were characterized by TEM, FTIR, zetasizer, and surface area calculations. The efficiency and selectivity of these Cd(II) ion imprinted pHEMAC nanoparticles were investigated both in deionized water and simulated gastrointestinal fluids. Adsorption studies were performed at different pH, temperature and initial concentrations of Cd(II) ion to determine optimum conditions. The selectivity studies were accomplished with Cd(II) ion imprinted and non-imprinted pHEMAC nanoparticles by using different metal ion solutions (i.e., copper, zinc, nickel, and lead). Langmuir and Freundlich isotherms were applied for these adsorption studies
- Published
- 2018
26. Molecularly Imprinted Quartz Crystal Microbalance Sensor (QCM) for Bilirubin Detection
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Çiğdem Çiçek, Fatma Yılmaz, Erdoğan Özgür, Handan Yavuz, and Adil Denizli
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molecular imprinting technique ,bilirubin ,QCM ,PHEMA ,nanosensor ,Biochemistry ,QD415-436 - Abstract
This study aims the preparation of a QCM sensor for the detection of bilirubin in human plasma. Bilirubin-imprinted poly-(2-hydroxyethyl methacrylate-N-methacryloyl-l-tryptophan methyl ester) (PHEMATrp) nanofilm (MIP) on the gold surface of a QCM chip was synthesized by the molecular imprinting technique. Meanwhile, the non-imprinted PHEMATrp (NIP) nanofilm was synthesized by the same experimental technique to examine the imprinting effect. Characterization of MIP and NIP nanofilms on the QCM chip surface was achieved by atomic force microscopy (AFM), ellipsometry, Fourier transform infrared spectrophotometry-attenuated total reflectance (FTIR-ATR) and contact angle measurements (CA). The observations indicated that the nanofilm was almost in a monolayer. Thereinafter, the imprinted and the non-imprinted QCM chips were connected to the QCM system to investigate kinetic and affinity properties. In order to examine the selectivity of the MIP-PHEMATrp nanofilm, competitive adsorption of bilirubin with cholesterol and estradiol was performed. Limit of detection (LOD) and limit of quantitation (LOQ) values were calculated as 0.45 μg/mL and 0.9 μg/mL, respectively.
- Published
- 2016
- Full Text
- View/download PDF
27. Inside Front Cover: Synthesis of molecularly imprinted magnetic nanoparticles for selective cytidine adsorption
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Adil Denizli, Emine Öngün, Handan Yavuz, and Semra Akgönüllü
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chemistry.chemical_compound ,Adsorption ,Front cover ,Materials science ,Chemical engineering ,chemistry ,Magnetic nanoparticles ,Cytidine - Published
- 2021
28. Contributors
- Author
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Semra Akgönüllü, Laura Anfossi, Elif Burcu Aydın, Muhammet Aydın, Monireh Bakhshpour, Nurgul K. Bakirhan, Nilay Bereli, S. Campuzano, Duygu Çimen, P. Dalmasso, Aylin Dedeoglu, Adil Denizli, Fabio Di Nardo, M. Eguílaz, Ece Eksin, Arzum Erdem, P. Gallay, Meric Goker, F. Gutierrez, Pinar Yilgor Huri, Sariye Irem Kaya, Sevinc Kurbanoglu, Jean-Louis Marty, Rupesh Mishra, A. Montemerlo, M. López Mujica, Sibel A. Ozkan, F. Perrachione, Serban Peteu, J.M. Pingarrón, Miroslav Pohanka, Cristina Polonschii, G.A. Rivas, M.C. Rodríguez, M.D. Rubianes, Frieder W. Scheller, Huseyin Senturk, Mustafa Kemal Sezgintürk, Ana Maria Titoiu, Merve Uca, Alina Vasilescu, P. Yáñez-Sedeño, Aysu Yarman, and Handan Yavuz
- Published
- 2021
29. Molecularly Imprinted Based Sensors for Detection of Allergens
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Handan Yavuz, Semra Akgönüllü, and Adil Denizli
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education.field_of_study ,Allergy ,biology ,medicine.diagnostic_test ,Chemistry ,Population ,Molecularly imprinted polymer ,Total ige ,medicine.disease ,Immunoglobulin E ,medicine.disease_cause ,Allergen ,Immunoassay ,Immunology ,medicine ,biology.protein ,education ,Molecular imprinting - Abstract
Allergy is a widespread disease effective 10%–20% of the general population all around the world. Allergies are chronic illnesses caused by the exposure of the respiratory system to dust or pollen, the skin to chemical materials, or the digestion system to particular foods. Immunoglobulin E (IgE) antibody plays a very important role in the development of allergies in humans. The high level of total IgE in human serum reflects the presence of allergic conditions. Test kits for commercially available serum IgE measurements are based on conventional immunoassay techniques. These techniques include time-consuming procedures, expensive instruments, and hazardous labels. Currently, molecularly imprinted polymer-based sensors are utilized as an alternative artificial receptor. MIP-based sensor systems for biological applications have been widely utilized in works on biomolecular interactions, with the change in sensor signals. Herein, the development of rapid, easy-to-use and cost-effective allergen detection methods based on mass sensitive, optic, electrochemical, and nanomaterials based sensors have been reported. Finally, we present sensor systems were investigated with a molecular imprinting technique for the detection of allergens as a marker of various allergies.
- Published
- 2021
30. Contributors
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Sinan Akgöl, Semra Akgönüllü, Zeynep Altintas, Elif Burcu Aydın, Muhammed Aydın, Monireh Bakhshpour, Nilay Bereli, M. Iqbal Bhanger, Nura Brimo, Duygu Çimen, Adil Denizli, Faezeh Ghorbanizamani, Emur Henden, Neslihan Idil, Raif İlktaç, Rüstem Keçili, Yiğit Kozalı, Kevser Kusat, Sakib Hussain Laghari, Bo Mattiasson, Najma Memon, Arben Merkoçi, Hichem Moulahoum, Işık Perçin, Merve Asena Özbek, Erdoğan Özgür, Muqsit Pirzada, Tahira Qureshi, Esma Sari, Yeşeren Saylan, Dilek Çökeliler Serdaroğlu, Buse Sevgi, Mustafa Kemal Sezgintürk, Muhammad Raza Shah, Suna Timur, Aykut Arif Topçu, Recep Üzek, Handan Yavuz, and Figen Zihnioglu
- Published
- 2021
31. Sensors for the Detection of Heavy Metal Contaminants in Water and Environment
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Handan Yavuz, Nilay Bereli, Duygu Çimen, and Adil Denizli
- Subjects
Pollutant ,Food chain ,Metal contamination ,Waste management ,Wastewater ,High selectivity ,Metal contaminants ,Environmental science ,Heavy metals ,Environmental pollution - Abstract
Environmental pollution by heavy metals is a global problem. The main sources of waste are industrial or domestic products and heavy metal–containing pesticides and fertilizers. Heavy metal contamination poses a serious danger to the human health and environment. Heavy metals are among the important environmental pollutants because of their toxic effects on plants, animals, and humans, even at very low concentrations. Moreover, they have non-biodegradable property and accumulation in the food chain. Currently available methods for determination of metal ion levels in water include chromatographic and spectroscopic methods. These chromatographic and spectroscopic methods are costly methods that require intense technical training because of their time-consuming and complicated procedures. The development of sensors for monitoring of toxic metals especially in water and environment is very important. The nano and functional material-based chemical sensors have been extensively employed for the detection of heavy metals. These sensors have advantages such as high selectivity, sensitivity, portability, on-site sensing capability, and improved performance of the devices. This chapter will outline the broad contours of heavy metals and the practical applications for their detection, with sensors, in the water and environment.
- Published
- 2021
32. Sensors for the Detection of Food Contaminants
- Author
-
Nilay Bereli, Handan Yavuz, Adil Denizli, and Duygu Çimen
- Subjects
Pollutant ,Food chain ,Waste management ,Agrochemical ,business.industry ,digestive, oral, and skin physiology ,Food processing ,Environmental science ,Contamination ,Pesticide ,business ,Food safety ,Food contaminant - Abstract
Food safety is a public health concern. Food contaminants could be metal ions, residues of pesticides, mycotoxins, drug residues, allergens, preservatives, and contaminants from food processing and packaging. The contamination hazards in the agricultural food chain include residues of agrochemicals and natural toxins. These chemical pollutants have serious effects on human health such as liver, kidney, and neurological syndromes even in the minimum limits. The food contamination with toxins and environmental pollutants occurs during the processing, packaging, preparing, storage, and transportation. Traditionally, food contaminants are detected by conventional analytical techniques. The other approach for detection of food contaminants is sensors. Sensors offer various advantages including simple use, high sensitivity and selectivity, user-friendly, on-site detection, rapid and low-cost operation. This chapter summarizes the different sensor applications in food contaminant detection and analysis.
- Published
- 2021
33. Commercial sensors for pathogen detection
- Author
-
Handan Yavuz, Duygu Çimen, Nilay Bereli, Adil Denizli, Semra Akgönüllü, and Monireh Bakhshpour
- Subjects
Pathogen detection ,Emerging technologies ,Computer science ,Specific detection ,Nanotechnology ,Biosensor ,Commercialization - Abstract
Biosensor technology enabled the specific detection of diverse biomolecules including pathogenic microorganisms in a rapid, sensitive, easy, and cheap manner in recent years. Emerging technologies like nanotechnology, lab-on-a-chip microdevices, etc., lead further development to obtain biosensors with much better performance. The easy and on-site use of biosensors without the necessity of specific trained stuff made their commercialization easier. In this chapter, the commercial biosensors for the detection of most-common disease causing pathogens and the recent studies with some leading biosensor companies are summarized.
- Published
- 2020
34. Molecularly imprinted polymer based quartz crystal microbalance sensor system for sensitive and label-free detection of synthetic cannabinoids in urine
- Author
-
Adil Denizli, Semra Akgönüllü, Handan Yavuz, Dilek Battal, and M. Serkan Yalcin
- Subjects
Indoles ,Polymers ,Surface Properties ,Biomedical Engineering ,Biophysics ,Nanoparticle ,02 engineering and technology ,Naphthalenes ,01 natural sciences ,Molecular Imprinting ,Contact angle ,Limit of Detection ,Electrochemistry ,Humans ,Detection limit ,Chromatography ,Aqueous solution ,Cannabinoids ,Illicit Drugs ,Chemistry ,010401 analytical chemistry ,Molecularly imprinted polymer ,General Medicine ,Quartz crystal microbalance ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Substance Abuse Detection ,Polymerization ,Quartz Crystal Microbalance Techniques ,Nanoparticles ,0210 nano-technology ,Selectivity ,Biotechnology - Abstract
Herein, we prepared a novel quartz crystal microbalance (QCM) sensor for synthetic cannabinoids (JWH-073, JWH-073 butanoic acid, JWH-018 and JWH-018 pentanoic acid,) detection. Firstly, the synthetic cannabinoid (SCs) imprinted (MIP) and non-imprinted (NIP) nanoparticles were synthesized by mini-emulsion polymerization system. The SCs-imprinted nanoparticles were first characterized by SEM, TEM, zeta-size and FTIR-ATR analysis and then were dropped onto the gold QCM surface. The SCs-imprinted QCM sensor was characterized by an ellipsometer, contact angle, and AFM. The limit of detection was found as 0.3, 0.45, 0.4, 0.2 pg/mL JWH-018, JWH-073, JWH-018 pentanoic acid and JWH-073 butanoic acid, respectively. The selectivity of the SCs-imprinted QCM sensor was shown by using JWH-018, JWH-018 pentanoic acid, JWH-073 and JWH-073 butanoic acid. According to the results, the SCs-imprinted QCM sensors show highly selective and sensitive in a broad range of synthetic cannabinoid concentrations (0.0005–1.0 ng/mL) in both aqueous and synthetic urine solutions.
- Published
- 2018
35. Prism coupler-based sensor system for simultaneous screening of synthetic glucocorticosteroid as doping control agent
- Author
-
Recep Üzek, Handan Yavuz Alagöz, Esma Sari, Adil Denizli, and Memed Duman
- Subjects
Detection limit ,Analyte ,Chromatography ,Materials science ,010401 analytical chemistry ,Metals and Alloys ,Nanoparticle ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,High-performance liquid chromatography ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Contact angle ,Prism coupler ,Elemental analysis ,Materials Chemistry ,Electrical and Electronic Engineering ,Surface plasmon resonance ,0210 nano-technology ,Instrumentation - Abstract
Profiling at GCs level is an important issue since it is a key factor to determine administration intent for either therapeutic or doping purpose. In this study, the sensing platform which is based on a combination of surface plasmon resonance system with molecularly imprinted nanoparticles was developed for monitoring of prednisolone level as a selected GCs analyte in plasma and urine. Fabricated sensing platform was utilized with ellipsometer and contact angle measurements followed by characterization of nanoparticles (NPs) by the means of structurally (i.e. FTIR and elemental analysis) and morphologically (i.e. TEM and Zeta-sizer analysis). The lowest limit of detection by PIN2 encoded molecularly imprinted nanoparticle was achieved as at 5.5 ppb and 8.9 ppb at pH 6.6 and 7.4, respectively. Competition kinetic assays were performed by using 17-α-ethynyl estradiol (EEST) and estradiol (EST). The reusability studies have been applied to the sensor with the best results and 92.8% recovery is achieved. Validation studies of the system were performed according to the standard procedure by high performance liquid chromatography (HPLC). The method was successfully applied to monitor PRD levels in body fluids (urine and plasma) as a natural sample.
- Published
- 2018
36. Molecularly imprinted cryogel membranes for mitomycin C delivery
- Author
-
Kemal Çetin, Pınar Öncel, Aykut Arif Topçu, Handan Yavuz, Adil Denizli, Fen-Edebiyat Fakültesi, Cetin, Kemal -- 0000-0002-7393-7377, and TOPCU, Aykut Arif -- 0000-0002-5434-4920
- Subjects
Materials science ,Bulk polymerization ,Cell Survival ,Surface Properties ,Scanning electron microscope ,Mitomycin ,Acrylic Resins ,Biomedical Engineering ,Biophysics ,Bioengineering ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Cell Line ,Polymerization ,Molecular Imprinting ,Biomaterials ,Mice ,Drug Delivery Systems ,Materials Testing ,medicine ,Animals ,Humans ,Fourier transform infrared spectroscopy ,Chromatography ,Mitomycin C ,Membranes, Artificial ,021001 nanoscience & nanotechnology ,In vitro ,0104 chemical sciences ,Drug Liberation ,Cross-Linking Reagents ,Membrane ,Drug delivery ,Cryogel Membrane ,Mitomycin C Delivery ,Adsorption ,Swelling ,medicine.symptom ,0210 nano-technology ,Porosity ,Cryogels - Abstract
WOS: 000399567500002, PubMed: 28105892, In this study, cryogel-based implantable molecularly imprinted drug delivery systems were designed for the delivery of antineoplastic agent. Mitomycin C imprinted poly(2-hydroxyethyl methacrylate-N-methacryloyl-l-glutamic acid) cryogel membranes were produced by free-radical bulk polymerization under partially frozen conditions. The membranes were characterized by swelling tests, Fourier transform infrared spectroscopy, scanning electron microscopy, surface area measurements and in vitro hemocompatibility tests. In vitro delivery studies were carried out to examine the effects of cross-linker ratio and template content. Mitomycin C imprinted cryogel membranes have megaporous structure (10-100 mu m in diameter). The cumulative release of mitomycin C was decreased with increasing cross-linking agent ratio and increased with the amount of template in the cryogel structure. The nature of transport mechanism of the mitomycin C from the membranes was non-Fickian., TUBITAK-BIDEB2211-National Ph.D. Scholarship Programme, Kemal Cetin thanks The Scientific and Technological Research Council of Turkey (TUBITAK) for the support by 'TUBITAK-BIDEB2211-National Ph.D. Scholarship Programme'.
- Published
- 2017
37. Surface-imprinted silica particles for Concanavalin A purification from Canavalia ensiformis
- Author
-
Galiya Razym, Monireh Bakhshpour, Çiğdem Kip, Ali Tuncel, Handan Yavuz, and Adil Denizli
- Subjects
Clinical Biochemistry ,030226 pharmacology & pharmacy ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,Molecular Imprinting ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Adsorption ,Concanavalin A ,Fourier transform infrared spectroscopy ,Polyacrylamide gel electrophoresis ,Chromatography ,biology ,010401 analytical chemistry ,Temperature ,Lectin ,Cell Biology ,General Medicine ,Hydrogen-Ion Concentration ,biology.organism_classification ,Silicon Dioxide ,0104 chemical sciences ,Canavalia ,chemistry ,Plant protein ,Canavalia ensiformis ,biology.protein ,Electrophoresis, Polyacrylamide Gel ,Lysozyme - Abstract
Concanavalin A is a representative of the plant protein group known as lectins. Many lectin proteins have useful characteristics for studies on cell division and cell surfaces. In this study, a new adsorbent for the specific separation of Concanavalin A was prepared by applying a silica particle surface imprinting method. First, silica particles were activated via acidic treatment, and then, 3-methacryloyloxypropyl trimethoxysilane (MPTMS) was used for modification. For the preparation of Concanavalin A surface-imprinted silica particles (Con A-MISPs), N-methacryloyl- l -histidine methyl ester (MAH) was used as a functional monomer. The silica particles were characterized using a Zetasizer, scanning electron microscopy equipment (SEM), and Fourier transform infrared spectroscopy (FTIR). The effects of parameters such as the pH, initial concentration of Concanavalin A, and temperature on the adsorption of Concanavalin A were determined. The maximum Concanavalin A adsorption onto Con A-MISPs was observed to be 305.2 mg/g at a pH of 6. The reusability of the Con A-MISPs was approximately 93.5%. The non-imprinted silica particles (NISPs) were prepared in the same manner without Concanavalin A to compare the surface imprinting factor. Selective binding studies were carried out with lysozyme and hemoglobin molecules. The selectivity of the Con A-MISPs was also investigated by isolating Concanavalin A from Canavalia ensiformis. The purity of the Concanavalin A was shown by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE).
- Published
- 2019
38. Introduction to Nanoscience, Nanomaterials, Nanocomposite, Nanopolymer, and Engineering Smart Materials
- Author
-
Necdet Sağlam, Yeşeren Saylan, Celal Ulger, Adil Denizli, and Handan Yavuz
- Subjects
Nanocomposite ,Materials science ,Nanosensor ,High surface area ,Nanobiotechnology ,High capacity ,Nanotechnology ,Smart material ,Nanoscopic scale ,Nanomaterials - Abstract
Biophysical properties of nanoscale objects like high surface area, high diffusivity to cells, efficient uptake, high capacity to be used in biological interfaces, etc. make them very useful tools. Bionanotechnology or nanobiotechnology overlaps nanotechnology in terms of generation of bio-inspired hybrid materials derived from chemical or biological synthesis to fabricate functional macromolecules. Microbial cells are ideal producers for such structures because of their controlled culturability, easily genetic manipulability, and wide diversity.
- Published
- 2019
39. Quartz crystal microbalance based histidine sensor
- Author
-
Adil Denizli, Erdoğan Özgür, Handan Yavuz, Merve Sönmezler, [Sonmezler, Merve -- Ozgur, Erdogan -- Yavuz, Handan -- Denizli, Adil] Hacettepe Univ, Dept Chem, Ankara, Turkey -- [Ozgur, Erdogan] Aksaray Univ, Dept Chem, Aksaray, Turkey, Sabire Yazıcı Fen Edebiyat Fakültesi, and Kimya
- Subjects
Materials science ,Kuvars Kristal Mikro Dengeleyici ,Biomedical Engineering ,Pharmaceutical Science ,Medicine (miscellaneous) ,Nanoparticle ,02 engineering and technology ,Biosensing Techniques ,Molecular İmprinting ,Contact angle ,Amino Asit ,03 medical and health sciences ,quartz crystal microbalance ,0302 clinical medicine ,Limit of Detection ,Monolayer ,Histidine ,Tayini Moleküler Baskı ,L-histidine ,technology, industry, and agriculture ,Proteins ,General Medicine ,Quartz crystal microbalance ,021001 nanoscience & nanotechnology ,Miniemulsion ,Polymerization ,Chemical engineering ,030220 oncology & carcinogenesis ,Quartz Crystal Microbalance Techniques ,Nanoparticles ,molecular imprinting ,Adsorption ,amino acid detection ,0210 nano-technology ,Molecular imprinting ,Biosensor ,Biotechnology - Abstract
WOS: 000457568600001, PubMed: 30686053, Herein, quartz crystal microbalance (QCM) biosensor is prepared for the detection of L-histidine by attachment of L-histidine imprinted poly(EGDMA-MAH/Cu(II)) nanoparticles on QCM electrode. The imprinted nanoparticles with the size of 86.43 nm were synthesized via miniemulsion polymerization reaction. Prepared QCM sensors were characterized with ellipsometer, contact angle measurements and FTIR. The thickness measurements demonstrated that the particle thin films were almost mono-layer. L-histidine solutions with a concentration range between 6.44 mu M and 225.6 mu M were introduced to QCM system to determine the adsorption kinetics. Selectivity of the L-histidine imprinted nanoparticles were examined using D-histidine and L-tryptophan as competitor molecules. L-histidine imprinted QCM biosensors was also used for RNAase, lysozyme, cytochrome-C and BSA to investigate the competitive adsorption of surface histidine exposed proteins.
- Published
- 2019
40. SPR nanosensor based on molecularly imprinted polymer film with gold nanoparticles for sensitive detection of aflatoxin B1
- Author
-
Handan Yavuz, Semra Akgönüllü, and Adil Denizli
- Subjects
Detection limit ,Aflatoxin ,Aflatoxin B1 ,Chemistry ,010401 analytical chemistry ,Molecularly imprinted polymer ,Metal Nanoparticles ,Nanotechnology ,02 engineering and technology ,Surface Plasmon Resonance ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,Molecular Imprinting ,Molecularly Imprinted Polymers ,Linear range ,Colloidal gold ,Nanosensor ,Humans ,Gold ,Surface plasmon resonance ,0210 nano-technology ,Plasmon - Abstract
Aflatoxins which are highly toxic, immunosuppressive and carcinogenic secondary metabolites produced naturally by Aspergillus flavus fungal species have a harm effect on human and animal health. Label free and rapid sensing of aflatoxin B1 (AFB1) has drawn the increased interest of highly sensitive and selective research. A highly sensitive and selective plasmonic sensing method was developed for the detection of AFB1 based on enhance-surface plasmon resonance nanosensor. Firstly, AFB1 and N-methacryloyl- l -phenylalanine were pre-complexed as a template molecule and functional monomer. Molecularly imprinted polymers with gold nanoparticles were coated onto surface plasmon resonance (SPR) gold chip surface. The AFB1 imprinted nanosensor shown a wide linear range, between 0.0001 ng mL−1 and 10.0 ng mL−1, and the limit of detection is 1.04 pg mL−1. Compared to the non-imprinted nanosensor, the imprinting factor was found to be 5.91. Also, detection studies of AFB1 were performed using various food samples. Finally, SPR nanosensors were performed selectivity, reusability and storage stability analysis.
- Published
- 2020
41. Preparation of imprinted cryogel cartridge for chiral separation of<scp>l</scp>-phenylalanine
- Author
-
Adil Denizli, Semra Akgönüllü, and Handan Yavuz
- Subjects
Materials science ,Surface Properties ,Phenylalanine ,Biomedical Engineering ,Pharmaceutical Science ,Medicine (miscellaneous) ,02 engineering and technology ,01 natural sciences ,Molecular Imprinting ,Cartridge ,Adsorption ,Molecule ,Chromatography ,Osmolar Concentration ,010401 analytical chemistry ,Temperature ,Water ,Stereoisomerism ,Fast protein liquid chromatography ,General Medicine ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Kinetics ,Membrane ,Racemic mixture ,0210 nano-technology ,Selectivity ,Cryogels ,Biotechnology - Abstract
l-Phe-imprinted cryogel cartridge was prepared for the chiral separation of l-Phe. N-Methacryloyl l-phenylalanine (MAPA) was used as a functional monomer for complexing with l-Phe. The selectivity of the membranes was investigated by using d-Phe, l-Trp, and d-Trp as competitor molecules. The PHEMAPA-l-Trp membranes were 6.4, 4.3, and 5.5 times more selective for l-Phe than d-Phe, l-Trp, and d-Trp, respectively. The PHEMAPA-l-Phe cryogel cartridge was incorporated into the fast protein liquid chromatography (FPLC) equipment and was able to separate D,l-Phe racemic mixture efficiently. The PHEMAPA-l-Phe membranes were shown to be reusable many times without significant loss of the adsorption capacity.
- Published
- 2016
42. Quartz crystal microbalance based biosensors for detecting highly metastatic breast cancer cells via their transferrin receptors
- Author
-
Seda Atay, Adil Denizli, Canan Çakır, Kevser Piskin, Fatma Yılmaz, and Handan Yavuz
- Subjects
chemistry.chemical_classification ,Chromatography ,General Chemical Engineering ,Ethylene glycol dimethacrylate ,010401 analytical chemistry ,technology, industry, and agriculture ,General Engineering ,Transferrin receptor ,02 engineering and technology ,(Hydroxyethyl)methacrylate ,Quartz crystal microbalance ,021001 nanoscience & nanotechnology ,01 natural sciences ,Receptor–ligand kinetics ,0104 chemical sciences ,Analytical Chemistry ,chemistry.chemical_compound ,chemistry ,Transferrin ,Monolayer ,0210 nano-technology ,Biosensor - Abstract
A quartz crystal microbalance (QCM) biosensor was developed to detect highly metastatic breast cancer cells by functionalizing the gold sensor surface with transferrin attachment. MDA-MB 231 breast cancer cells with high and MCF 7 cells with low metastatic potential and transferrin expression were used. Serum starved MDA-MB-231 cells were used as control cells. First, poly(2-hydroxyethyl methacrylate) (PHEMA) nanoparticles were prepared by mini-emulsion polymerization of hydroxyethyl methacrylate (HEMA) and ethylene glycol dimethacrylate (EGDMA). Nanoparticles were characterized with a zeta-sizer and then their suspension is dropped on the surface of the QCM and the dried QCM surface was modified further by activation with carbodiimide and transferrin attachment. The QCM biosensor was analyzed by using atomic force microscopy (AFM), ellipsometry, Fourier transform infrared spectrophotometry (FTIR) and contact angle measurements. The cells were applied to the derivatized QCM sensor to investigate the affinity and binding kinetics. The nanoparticles and transferrin were found to form a monolayer on the QCM surface. Binding kinetics were best fitted to the Langmuir–Freundlich adsorption model. The QCM signal was correlated well with the number of transferrin receptors on cells. It is concluded that the QCM biosensor functioning via transferrin receptor interactions may be used to detect breast cancer cells with high metastatic potential.
- Published
- 2016
43. Rapid and sensitive detection of synthetic cannabinoids JWH-018, JWH-073 and their metabolites using molecularly imprinted polymer-coated QCM nanosensor in artificial saliva
- Author
-
M. Serkan Yalcin, Handan Yavuz, Semra Akgönüllü, Adil Denizli, and Dilek Battal
- Subjects
chemistry.chemical_classification ,Detection limit ,Chromatography ,Molecularly imprinted polymer ,Nanoparticle ,Quartz crystal microbalance ,Polymer ,Analytical Chemistry ,chemistry ,Nanosensor ,Synthetic cannabinoids ,medicine ,NIP ,Spectroscopy ,medicine.drug - Abstract
Synthetic cannabinoids (SCs), which are becoming increasingly popular, are an important public health issue considering the common abuse uses and serious adverse effects associated with intoxication. The analysis for controlling the increase in synthetic cannabinoids usage requires a faster and highly sensitive detection that rapidly developing class compounds. In this study, a piezoelectric nanosensor coated with a synthetic biomimetic recognition nanoparticles polymer was designed for the real-time and highly sensitive synthetic cannabinoids (SCs: JWH-018, JWH-073, JWH-018 pentanoic acid and JWH-073 butanoic acid) detection. Synthetic cannabinoids imprinted (MIP) and non-imprinted (NIP) nanoparticles were synthesized. Firstly, the characterization of MIP and NIP nanoparticles were studied by FTIR-ATR, scanning electron microscope, and size measurements. After, nanoparticles were spread onto a quartz crystal microbalance (QCM) chip. Different QCM chip surfaces were studied by contact angle, ellipsometry, and atomic force microscopy measurements. Selective rebinding of target analytes was monitored as a frequency shift measuring mass change with the QCM. Limit of detection values were calculated as 0.28, 0.3, 0.23, 0.29 pg/mL for these SCs in artificial saliva, respectively. The SCs-MIP QCM nanosensors displayed high sensitivity and selectivity in a wide concentration range of SCs (0.0005–1.0 ng/mL) in artificial saliva.
- Published
- 2020
44. HbA1c detection via high-sensitive boronate based surface plasmon resonance sensor
- Author
-
Monireh Bakhshpour, Handan Yavuz, Adil Denizli, and Merve Çalışır
- Subjects
02 engineering and technology ,010402 general chemistry ,01 natural sciences ,High-performance liquid chromatography ,chemistry.chemical_compound ,Valine ,Materials Chemistry ,medicine ,Electrical and Electronic Engineering ,Instrumentation ,Chromatography ,Chemistry ,Metals and Alloys ,Carbohydrate ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Human serum albumin ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Glucose binding ,Hemoglobin ,0210 nano-technology ,Selectivity ,Boronic acid ,medicine.drug - Abstract
Glycosylated Hemoglobin, known as HbA1c, is the most commonly used molecule to track and diagnose the Type II diabetes and gives very convincing accurate results. Tracking the HbA1c is also an early diagnostic tool to indicate diabetes occurrence in high risk patience. Glycosylated hemoglobin is the result of glucose binding of the β-chain of hemoglobin to N-terminal valine and reflects the average glucose value over the past 2–3 months. HbA1c in the blood concentration of more than 141 mg/dL is often enough to diagnose diabetes. Boronic acid derivatives are often used in HbA1c determination due to the carbohydrate relationship based on the cis-diol interaction and determinations based on this association are mostly carried out by enzymatic sensors and HPLC. In addition to these methods, sensor studies have also started to be developed as an alternative. In this study, it is aimed to determine HbA1c by a surface plasmon resonance sensor (SPR) modified with a boronic acid derivative vinyl phenyl boronic acid. This study is shown that the pH value is an important parameter for binding and the signal received is increased as the concentration increases. Even at low concentrations like 10 μg/mL, signal can be received and it implies that more accurate measurement can be made at clinical concentration values which are higher. In artificial plasma studies, different sensograms are obtained for human serum albumin, immunoglobulin G, and hemoglobin molecules which all could bind to modified chip and the selectivity to the molecules are distinctively differentiated in comparison with HbA1c.
- Published
- 2020
45. Surface plasmon resonance aptasensor for detection of human activated protein C
- Author
-
Adil Denizli, Arzum Erdem, Seda Koyun, Semra Akgönüllü, and Handan Yavuz
- Subjects
Aptamer ,education ,02 engineering and technology ,01 natural sciences ,Analytical Chemistry ,Contact angle ,chemistry.chemical_compound ,Limit of Detection ,Humans ,Bovine serum albumin ,Surface plasmon resonance ,Detection limit ,Chromatography ,biology ,Base Sequence ,010401 analytical chemistry ,Aptamers, Nucleotide ,Surface Plasmon Resonance ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Kinetics ,Monomer ,Myoglobin ,chemistry ,biology.protein ,0210 nano-technology ,Selectivity ,Protein C - Abstract
The aim of this study is a highly sensitive and selective label-free surface plasmon resonance (SPR) aptasensor preparation for the specific detection of human activated protein C (APC). In the first step, DNA aptamer was complexed with N-methacryloyl-L-cysteine (MAC) monomer. Then, cyanamide and 2-hydroxyethyl methacrylate solution was mixed with the DNA-Apt/MAC complex. Two different SPR sensors (Random-DNA and HEMA-MAC polymeric films) were also prepared by following the same experimental procedure. The characterization of SPR aptasensors was done by contact angle, atomic force microscopy, and ellipsometer analysis. Selectivity studies of SPR aptasensors were performed in the presence of bovine serum albumin, hemoglobin and myoglobin. Desorption studies were performed by using 0.025 M NaCl solution. The limit of detection (LOD) and limit of quantification (LOQ) values of DNA-Apt SPR aptasensor was determined as 1.5 ng/mL and 5.2 ng/mL.
- Published
- 2018
46. Evaluation of hydrochar efficiency for simultaneous removal of diclofenac and ibuprofen from aqueous system using surface response methodology
- Author
-
Najma Memon, Abdesadek Lachgar, Tahira Qureshi, Adil Denizli, Handan Yavuz, and Saima Q. Memon
- Subjects
Sorbent ,Diclofenac ,Central composite design ,Dried fruit ,Health, Toxicology and Mutagenesis ,Ibuprofen ,010501 environmental sciences ,Wastewater ,01 natural sciences ,Waste Disposal, Fluid ,Adsorption ,Environmental Chemistry ,Response surface methodology ,Particle Size ,0105 earth and related environmental sciences ,Aqueous solution ,Chemistry ,Sorption ,General Medicine ,Pollution ,Carbon ,Kinetics ,Charcoal ,Particle size ,Water Pollutants, Chemical ,Nuclear chemistry - Abstract
Pharmaceutically active compounds like diclofenac (DFS), ibuprofen (IBP), and other drugs that persist in the environment are listed as emerging contaminants. These escape from normal wastewater treatment plants and find their way to water streams; therefore, alternate treatment processes are needed. Herein, a sorbent material is reported that is prepared through hydrotermal carbonization from dried fruit powder of Zizipus mauritiana L. (HTC-ZM) and applied for simultaneous removal of DFS and IBP. Carbonized material (HTC-ZM) was found as agglomerates of approximately 1 μm particle size with surface area of 1160 m2/g having oxygen functional groups (e.g., COO, O, C=O) on surface. Simultaneous removal of IBP and DFS onto HTC-ZM was studied using response surface methodology with a set of 18 experiments using factors such as pH, amount of sorbent, contact time, and sorbate concentration. Maximum removal efficiency was obtained 88% and 97% for DFS and for IBP, respectively, with adsorption capacity of 2.03 mmol g−1 for DFS and 2.54 mmol g−1 for IBP. Kinetics modeling and “mean free energy” values predicted that sorption is mainly governed by physical interactions followed by “pore filling” mechanism for uptake of DFS and IBP.
- Published
- 2018
47. Controlled release of mitomycin C from PHEMAH-Cu(II) cryogel membranes
- Author
-
Adil Denizli, Monireh Bakhshpour, and Handan Yavuz
- Subjects
Models, Molecular ,Mitomycin ,Biomedical Engineering ,Molecular Conformation ,Pharmaceutical Science ,Medicine (miscellaneous) ,02 engineering and technology ,010402 general chemistry ,Methacrylate ,01 natural sciences ,Cell Line ,Polymerization ,Molecular Imprinting ,chemistry.chemical_compound ,Polymethacrylic Acids ,Histidine ,digestive, oral, and skin physiology ,Mitomycin C ,Membranes, Artificial ,General Medicine ,021001 nanoscience & nanotechnology ,Controlled release ,0104 chemical sciences ,Kinetics ,Monomer ,Membrane ,chemistry ,Delayed-Action Preparations ,Suspension polymerization ,0210 nano-technology ,Molecular imprinting ,Copper ,Cryogels ,Biotechnology ,Nuclear chemistry - Abstract
Molecular imprinting technique was used for the preparation of antibiotic and anti-neoplastic chemotherapy drug (mitomycin C) imprinted cryogel membranes (MMC-ICM). The membranes were synthezied by using metal ion coordination interactions with N-methacryloyl-(l)-histidine methyl ester (MAH) functional monomer and template molecules (i.e. MMC). The 2-hydroxyethyl methacrylate (HEMA) monomer and methylene bisacrylamide (MBAAm) crosslinker were used for the preparation of mitomycin C imprinted cryogel membranes by radical suspension polymerization technique. The imprinted cryogel membranes were characterized by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) and swelling degree measurements. Cytotoxicity of MMC-ICMs was investigated using mouse fibroblast cell line L929. Time-dependent release of MMC was demonstrated within 150 h from cryogel membranes. Cryogels demonstrated very high MMC loading efficiency (70-80%) and sustained MMC release over hours.
- Published
- 2018
48. List of Contributors
- Author
-
Semra Akgönüllü, Cláudia Amaral, Dilek Battal, Oguz Bayraktar, Mariana Caldas, Raúl Cazorla-Luna, Kemal Çetin, Ramesh Chandra, Beata Chudzik-Rząd, Manuel A.N. Coelho, Grazia M.L. Consoli, Nily Dan, Costas Demetzos, Adil Denizli, Leonardo M.B. Ferreira, Ana Figueiras, Corrada Geraci, Giuseppe Granata, Maria Palmira D. Gremião, Nidhi Gupta, Hiroshi Handa, Özge K. Heinz, Aleksandr I. Ilin, Muhammad Imran, Sougata Jana, Masaaki Kawano, Sepideh Khoee, Charlene P. Kiill, Merve D. Köse, R. Mankamna Kumari, Bibek Laha, Yuri Lvov, Mariana Magalhães, Sabyasachi Maiti, Araceli Martín-Illana, Masanori Matsui, Surendra Nimesh, Fernando Notario-Pérez, Akram Nouri, Pravin Pattekari, Liliane N. Pedreiro, Maria C. Pereira, Natassa Pippa, Stergios Pispas, Maria J. Ramalho, Andreza M. Ribeiro, António José Ribeiro, Carlos Fontes Ribeiro, Roberto Ruiz-Caro, Aline M. Santos, Ana Cláudia Santos, null Shafiullah, Muhammad R. Shah, Nikita Sharma, Jan Sobczyński, Francisco Veiga, María-Dolores Veiga, Handan Yavuz, Gulnara A. Yuldasheva, and Georgii M. Zhidomirov
- Published
- 2018
49. Therapeutic protein and drug imprinted nanostructures as controlled delivery tools
- Author
-
Adil Denizli, Semra Akgönüllü, Dilek Battal, Kemal Çetin, and Handan Yavuz
- Subjects
Drug ,chemistry.chemical_classification ,Nanostructure ,Biocompatibility ,Chemistry ,media_common.quotation_subject ,Nanoparticle ,Nanotechnology ,02 engineering and technology ,Polymer ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Small molecule ,0104 chemical sciences ,Drug delivery ,0210 nano-technology ,Molecular imprinting ,media_common - Abstract
Polymers play an enormous role in the drug delivery field owing to their flexibility, biocompatibility, and controllable size properties. With the advances in the polymer technology and nanotechnology, the novel drug delivery systems (DDSs) based on nanoparticles have been successfully developed. Nanoparticulate DDSs with an enhanced permeation allow targeted delivery of an intended therapeutic drug to the site of action with predetermined time and activity. Numerous applications have been reported which use nanoparticles as smart delivery systems for synthetic, semi-synthetic or natural drugs, which could be small molecules or huge molecules like proteins. In smart DDSs the critical point is to achieve tunable drug release. Diffusion, matrix degradation or both can control this property. In recent years the use of molecular imprinting technology to arrange the drug release properties from the polymer matrix is attracting significant attention. The basis of this approach is the affinity of an imprinted polymer for the drug molecule, which provides high loading capacity and sustained release profiles. Imprinting also allows for construction of responsive drug release systems based on competitive binding of the imprinted molecule with the weakly bound drug. The studies on the nanoparticle-based imprinted delivery systems will be summarized in this chapter.
- Published
- 2018
50. Purification of transferrin by magnetic immunoaffinity beads
- Author
-
Dilara Saçlıgil, Serap Şenel, Adil Denizli, and Handan Yavuz
- Subjects
chemistry.chemical_classification ,Dispersion polymerization ,Thermogravimetric analysis ,Glycidyl methacrylate ,Aqueous solution ,Chromatography ,Filtration and Separation ,Analytical Chemistry ,law.invention ,chemistry.chemical_compound ,Adsorption ,chemistry ,Transferrin ,law ,Fourier transform infrared spectroscopy ,Electron paramagnetic resonance - Abstract
Immunoaffinity adsorbent for transferrin (Tf) purification was prepared by immobilizing anti-transferrin (Anti-Tf) antibody on magnetic monosizepoly(glycidyl methacrylate) beads, which were synthesized by dispersion polymerization technique in the presence of Fe3 O4 nanopowder and obtained with an average size of 2.0 μm. The magnetic poly(glycidyl methacrylate) (mPGMA) beads were characterized by Fourier transform infrared spectroscopy, swelling tests, scanning electron microscopy, electron spin resonance spectroscopy, thermogravimetric analysis and zeta sizing analysis. The density and swelling ratio of the beads were 1.08 g/cm(3) and 52%, respectively. Anti-Tf molecules were covalently coupled through epoxy groups of mPGMA. Optimum binding of anti-Tf was 2.0 mg/g. Optimum Tf binding from aqueous Tf solutions was determined as 1.65 mg/g at pH 6.0 and initial Tf concentration of 1.0 mg/mL. There was no remarkable loss in the Tf adsorption capacity of immunoaffinity beads after five adsorption-desorption cycles. Tf adsorption from artificial plasma was also investigated and the purity of the Tf molecules was shown with gel electrophoresis studies.
- Published
- 2015
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