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Prosopis strombulifera (Lam.) Benth. is a halophytic shrub abundant in high-salinity areas in central Argentina, with high tolerance against NaCl but strong growth inhibition by Na2SO4. In the present study, the modulation of the antioxidant systems (enzymatic and non-enzymatic components) was analyzed under different salt treatments (NaCl, Na2SO4 and the iso-osmotic mixture) in hydroponic cultivation. Na2SO4-treated plants showed strong indications of oxidative stress (H2O2 and O2-• increase). Modifications in antioxidant enzymes activities were observed mainly under Na2SO4 treatment, where CAT seems to play an important role in early detoxification of H2O2 in roots, whereas SOD and APX have a predominant role in leaves. As part of the non-enzymatic system, 21 compounds were identified in leaves, being polyphenols the most abundant. Control plants contained the major variety of detected phytochemicals (14). Na2SO4-treated plants contained 10 compounds and NaCl-treated plants nine compounds, but with a different profile. NaCl-treated plants showed the highest antioxidant capacity. Our findings confirm that different types of salt treatments provoke a differential modulation of the antioxidant systems. Polyphenols and other ROS-detoxifying compounds, in a joint action with the enzymatic antioxidant system, are proposed to have a fundamental role in the cellular protection of P. strombulifera plants under severe oxidative stress. Our findings also highlight the potential of this halophyte as a valuable source of bioactive compounds with high antioxidant activity and health benefits.

Saponins are natural surfactant compounds whose aqueous solutions can form stable foam and micellar solutions. Gypsophila arrostii Guss., Caryophyllaceae, represents the main source of saponins for industrial applications, such as vaccine adjuvants. Due to the complex and amphiphilic nature of triterpene saponins, these compounds require sophisticated techniques for their isolation and characterization. The hydromethanolic saponin fractions from the roots of the analyzed plant material were investigated by electrospray ionization ion trap multiple-stage mass spectrometry (ESI-IT-MS) in negative mode. According to the m/z values for the deprotonated molecule [M-H]− and the characteristic product ions, four known Gypsophila-type saponins were successfully characterized. In addition, four undescribed saponin compounds were detected by the registered ions [M-H]−.

Immunoadjuvants have major effects on the immune responses in vaccine formulations. Many specific adjuvants have been studied, including mineral salts, emulsions, cytokines, non-ionic block copolymers, carbohydrate polymers, muramyl dipeptides, and saponin-based adjuvants. Here, we focus on quillaic acid–containing saponin-based adjuvants and their mechanisms of action. Interestingly, when analyzing the adjuvants capable of forming immune-stimulating complexes, one of the characteristics shared by the majority of them is the presence of quillaic acid as the aglycone. This suggests that quillaic acid might be a strategic molecule for immunoadjuvant activity and a powerful candidate in the development of novel adjuvants.

Saponins are commonly found in higher plant parts, particularly in roots, tubers, leaves, and seeds, but N-triterpene saponins are rarely found in nature. Here, we focus on molecular structures and anticancer properties particularly in synergistic effects of toxicity, ion channel inhibitory, and opioid receptor modulatory effects of N-triterpene saponins.

Nanoemulsions are an isotropical mixture of oil, surfactant, and water with droplet diameter approximately in the range of 10-100 nm. They are being exponentially used for drug delivery systems for the influential administration of therapeutical agents because of their potential advantages over other approaches. Nanoemulsions can be used to design delivery systems that have increased drug loading, enhanced drug solubility, increased bioavailability, controlled drug release, and enhanced protection against chemical or enzymatic degradation. Moreover, nanoemulsions have better thermodynamical stability to flocculation, sedimentation, and creaming than conventional emulsions. Their small droplet dimensions and large droplet surface area positively influence drug transport and delivery, along with allowing targeting to specific sites. This chapter focuses on recent applications of nanoemulsions in the area of drug delivery.

The study was aimed to evaluate the inhibitory activity towards angiotensin converting enzyme I (ACE-I), antioxidant capacity and phenolics of Alchemilla vulgaris (lady’s mantle) naturally grows in Turkey. Aqueous extracts of lady’s mantle were used for enzyme inhibition assay on angiotensin converting enzyme I purified from sheep lungs. Trolox equivalent antioxidant capacity (TEAC) and DPPH assays were also used for monitoring of antioxidant profile of methanolic and aqueous extracts. Total phenolics of the extracts from lady’s mantle were also analyzed. Our research results indicated that lady’s mantle could have important anti-hypertensive effects due to their potent inhibitory activity towards angiotensin converting enzyme I.

SARS-CoV-2 is a novel coronavirus with a major outbreak of unusual viral epidemic in Wuhan, China. To date, some certain vaccines have been approved by FDA so that spelling the end of pandemic globally but COVID-19 is still a global leading cause of death, with a descending rates. The existence of antiviral properties of ribosome inactivating proteins towards many human, animal and plant viruses are well-known for so many years. Ribosome inactivating proteins are naturally occurring substances and toxic N-glycosidase enzymes which depurinate both eukaryotic and prokaryotic rRNAs, hence arresting protein synthesis in the coarse of translation. Interestingly, some ribosome inactivating proteins can also remove the purine nucleotides from non-ribosomal RNAs. Undoubtly, removing the rRNA purine nucleotides effects the protein synthesis and therefore, triggers ribotoxic stress response that commences apoptosis. These proteins might similarly hamper specifically the viral circles in one and/or more points in their lifecycle. In present study, we have focused on naturally occurring ribosome inactivating proteins as promising agents for struggle against Covid-19 disease.

Prosopis strombulifera (Lam.) Benth. is a halophytic shrub abundant in high-salinity areas in central Argentina, with high tolerance against NaCl but strong growth inhibition by Na

Saporin, a type I ribosome-inactivating protein from soapwort plant, is a potent protein synthesis inhibitor. Catalytically, saporin is a characteristic N-glycosidase, and it depurinates a specific adenine residue from a universally conserved loop of the major ribosomal RNA (rRNA) of eukaryotic cells. It is well-known that saporin induces apoptosis through different pathways, including ribotoxic stress response, cell signal transduction, genomic DNA fragmentation and RNA abasic lyase (RAlyase) activity, and NAD+ depletion by poly-(ADP)-ribose polymerase hyperactivation. Saporin's high enzymatic activity, high stability, and resistance to conjugation procedures make it a well-suited tool for immunotherapy approaches.In the present study, we focus on saporin-based targeted toxins that may be efficacious therapeutic agents for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Our discussed points suggest that saporin may be a strategic molecule for therapeutic knockout treatments and a powerful candidate for novel drugs in the struggle against coronavirus 2019 (COVID-19).

Aims: Nondiabetic patients were studied to determine whether modest elevations in plasma mannose may be associated with a greater incidence of coronary artery disease (CAD). Materials and Methods: Plasma insulin, mannose, glucose, hexokinase 1-2, GLUT1-GLUT4 levels, and serum mannose phosphate isomerase enzyme levels were evaluated with respect to subsequent CAD using records from 120 nondiabetic CAD patients and 120 healthy volunteers. CAD was identified from myocardial infarction and new diagnoses of angina. Results: Of 120 nondiabetic CAD patients studied, their plasma GLUT4 and HK1 levels were significantly lower than those of the control group. In addition, a significant increase in plasma mannose levels was found in the patient group compared to the control group. Conclusion: Our findings showed that elevated baseline mannose levels in plasma are associated with an increased risk of CAD over time. © Copyright 2020, Mary Ann Liebert, Inc., publishers 2020.

Natural products from fungi, especially Ascomycota, play a major role in therapy and drug discovery. Fungal strains originating from marine habitats offer a new avenue for finding unusual molecular skeletons. Here, the marine-derived fungus Epicoccum nigrum (strain 749) was found to produce the azaphilonoid compounds acetosellin and 5′,6′-dihydroxyacetosellin. The latter is a new natural product. The biosynthesis of these polyketide-type compounds is intriguing, since two polyketide chains are assembled to the final product. Here we performed 13 C labeling studies on solid cultures to prove this hypothesis for acetosellin biosynthesis. © 2017 Georg Thieme Verlag KG Stuttgart. New York.

The maintenance of lipid and fatty acids unsaturated composition has been described as one of the mechanisms associated to drought tolerance, but research about the lipid profile in native plants of semiarid environment is still limited. The primary objective was to study whether lipid profiles correlates with drought resistance strategies (tolerant or avoidant) of two life forms (shrubs and grasses). The lipid classes and molecular species of green leaves of Larrea divaricata and Lycium chilense shrubs and Pappostipa speciosa and Poa ligularis grasses were determined using LC–ESI-MS/MS. The soil water content was very low during spring and leaf relative water content was between 47 and 74% in the four species. Lipid profiling was different between both life forms. The prevalent compounds were digalactosyldiacylglycerol (DGDG), monogalactosyldiacylglycerol (MGDG) and phosphatidic acid (PA). The lipid signature shows that L. divaricata adjust its lipid composition to tolerate drought, increasing the content of: a) total lipids and total phospholipids, b) structural phospholipids (36:4 and 36:2-PC, phosphatidylcholine; 36:4-PE, phosphatidylethanolamine), c) chloroplast and mitochondria lipids (32:1 and 32:0-PG, phosphatidylglycerol; 34:3, 36:6 and 36:3-DGDG), d) signaling lipids (34:3, 34:2 and 36:5-PA and PI, phosphatidylinositol), and e) polyunsaturated fatty acids (PUFAs, 18:3 and 18:2) and long chain polyunsaturated fatty acids (LC-PUFAs, in 40:2 and 42:2-PS, phosphatidylserine). This membrane lipid composition contributes to membrane stabilization as metabolic-functional strategy for drought tolerance in the Patagonian Monte. In addition, the 18:3 present in lipids of both grasses could be incorporated to lamb fed based on pastures and result healthy for human dietary. © 2019, National Institutes of Health: P20 RR16475 Kansas State University National Center for Research Resources PIP-CONICET191/2011 Kansas NSF EPSCoR: EPS-0236913 National Sleep Foundation: MCB 0455318, 0920663, DBI 0521587 PUE-IPEEC 044/2016, Lipid analysis was performed at the Kansas Lipidomics Research Center (KLRC). Instrument acquisition and method development at KLRC was supported by NSF grants MCB 0455318 and 0920663 and DBI 0521587 , and NSF EPSCoR grant EPS-0236913 with matching support from the State of Kansas through Kansas Technology Enterprise Corporation and Kansas State University . The KLRC is also supported by K-INBRE (NIH Grant P20 RR16475 from the INBRE program of the National Center for Research Resources). This research was funded by the National Research Council of Argentina ( PIP-CONICET191/2011 ), Instituto Patagónico para el Estudio de los Ecosistemas Continentales ( PUE-IPEEC 044/2016 ) and the Ministry of Science, Technology and Innovation of Chubut Province (Res. Nº 37/10 ). I thank to Fundación Patagonia Natural who allowed me to harvest plant material on his Wildlife Reserve “La Esperanza”.

Saponins are amphiphilic glycoconjugates which give soap-like foams in H2O. A new triterpenoid saponin, simenoside A (1), based on gypsogenin aglycone, and the known saponin 2 were isolated from Gypsophila simonii Hub.-Mor. The structure of the new saponin was elucidated as 3-O-ß-D-galactopyranosyl-(1›2)-[ß-D-xylopyranosyl-(1›3)] -ß-D-glucuronopyranosylgypsogenin 28-O-ß-D-glucopyranosyl-(1›3)- [ß-D-glucopyranosyl-(1›2)-ß-D-xylopyranosyl-(1›4)] -?-L-rhamnopyranosyl-(1›2)-ß-D-fucopyranosyl ester on the basis of extensive spectral analyses and chemical evidence. Saponins 1 and 2 were isolated from G. simonii for the first time. Copyright © 2014 Verlag Helvetica Chimica Acta AG, Zürich.

The present study established baseline data on hemolytic and antioxidant capacity of saponin rich fractions (SRFs) of Gypsophila arrostii, G. pilulifera and G. simonii (Caryophyllaceae) naturally found in Turkey. The antioxidant activity of the each SRF was carried out using 2 different methods: free-radical scavenging activity using 2,2-diphenyl-1-picryl hydrazyl (DPPH) and ABTS assay. Hemolytic activity of SRFs was tested using diluted sheep bloods and saline/distilled water as control groups. Also, total phenolic contents of each fraction were determined. Our results demonstrated that G. arrostii, G. pilulifera and G. simonii possessed strong antioxidant and the slight hemolytic activity when comparing the other saponin containing extracts. (C) 2013 The Authors. Published by Elsevier B.V.

Summary: Plants exposed to drought stress, as usually occurs in Patagonian shrublands, have developed different strategies to avoid or tolerate the lack of water during their development. Production of phenolic compounds (or polyphenols) is one of the strategies used by some native species of adverse environments to avoid the oxidative damage caused by drought. In the present study the relationship between phenolic compounds content, water availability and oxidative damage were evaluated in two native shrubs: Larrea divaricata (evergreen) and Lycium chilense (deciduous) of Patagonian shrublands by their means and/or by multivariate analysis. Samples of both species were collected during the 4 seasons for the term of 1 year. Soil water content, relative water content, total phenols, flavonoids, flavonols, tartaric acid esters, flavan-3-ols, proanthocyanidins, antioxidant capacity and lipid peroxidation were measured. According to statistical univariate analysis, L. divaricata showed high production of polyphenols along the year, with a phenolic compound synthesis enhanced during autumn (season of greatest drought), while L. chilense has lower production of these compounds without variation between seasons. The variation in total phenols along the seasons is proportional to the antioxidant capacity and inversely proportional to lipid peroxidation. Multivariate analysis showed that, regardless their mechanism to face drought (avoidance or tolerance), both shrubs are well adapted to semi-arid regions and the phenolic compounds production is a strategy used by these species living in extreme environments. The identification of polyphenol compounds showed that L. divaricata produces different types of flavonoids, particularly bond with sugars, while L. chilense produces high amount of non-flavonoids compounds. Synthesis: These results suggest that flavonoid production and accumulation could be a useful indicator of drought tolerance in native species. © 2016 Elsevier Masson SAS.

A new acylated and triterpenoidal saponin, named GS1, was isolated from the roots of Gypsophila arrostii Guss. On the basis of acid hydrolysis, comprehensive spectroscopic analyses and comparison with spectral data of known compounds, its structure was established as 3-O-β-D-xylopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopyranosyl-{21-O-[(E)-3,4,5trimethoxycinnamoyl]}21-hydroxygypsogenin 28-O-β-D-glucopyranosyl-(1→2)- [β-D-arabinopyranosyl-(1→3)]-β-D-xylopyranosyl-(1→3]-α-L-rhamnopyranosyl ester. This article deals with the isolation and structural elucidation of new acylated and oleanane-type saponin.

The chemical composition of the essential oils of Eryngium campestre, E. thorifolium, and E. creticum (Apiaceae), growing in the Aegean region of Turkey (Mount Sandras, Denizli), was determined by direct thermal desorption (DTD)-GC/MS analyses. A total of 49 components were identified in the oils, ?-pinene and hexanal being the major compounds. The three essential oils were also tested for their inhibitory activity of nine different methicillin-resistant Staphylococcus aureus (MRSA) strains by the agar disc diffusion method. The anti-MRSA activity of E. thorifolium oil, the most active of the three oils, was comparable with those of the reference antibiotic vancomycin and oregano oil, although somewhat lower. © 2011 Verlag Helvetica Chimica Acta AG.

We investigated the genotoxic effect of nebuloside-A on whole blood DNA by using alkaline Single Cell Gel Electrophoresis Comet assay. Saponins are a widespread class of bioactive compounds produced by many plant species. Nebuloside A was isolated from babys-breath (Gypsophila arrostii) by high performance liquid chromatography method. Mass spectra obtained by electrospray ionization-time of flight-mass spectrometry in a negative ion mode to reduce the protonation. Complete nuclear magnetic resonance spectroscopy assignments of nebuloside A was achieved by using 2D-nuclear magnetic resonance spectroscopy techniques, such as double-quantum filtered correlation spectroscopy, heteronuclear single quanthum correlation, heteronuclear multiple-bond correlation, and total correlation spectroscopy. DNA damage was measured in total arbitrary units by visual scoring of comets in different concentrations of nebuloside A and H2O2 treatments. We found that significant dose dependent relationship between nebuloside A and its genotoxic effect. © 2015 Taylor & Francis Group, LLC.

The marine sponge-derived fungus Auxarthron reticulatum produces the cannabinoid receptor antagonist amauromine (1). Recultivation of the fungus to obtain further amounts for more detailed pharmacological evaluation of 1 additionally yielded the novel triterpene glycoside auxarthonoside (2), bearing, in nature, a rather rare sugar moiety, i.e., N-acetyl-6-methoxy-glucosamine. Amauromine (1), which inhibited cannabinoid CB1 receptors (Ki0.178µM) also showed antagonistic activity at the cannabinoid-like orphan receptor GPR18 (IC50 3.74µM). The diketopiperazine 1 may thus serve as a lead structure for the development of more potent and selective GPR18 antagonists, which are required to study the orphan receptor's potential as a new drug target. Despite the execution of many biological assays, to date, no bioactivity could be found for auxarthonoside (2). © Georg Thieme Verlag KG Stuttgart · New York.

Saponins are amphiphilic glycoconjugates which give soap-like foams in H2 O. A new triterpenoid saponin, simenoside A (1), based on gypsogenin aglycone, and the known saponin 2 were isolated from Gypsophila simonii Hub.-Mor. The structure of the new saponin was elucidated as 3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosylgypsogenin 28-O-β-D-glucopyranosyl-(1→3)-[β-D-glucopyranosyl-(1→2)-β-D-xylopyranosyl-(1→4)]-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranosyl ester on the basis of extensive spectral analyses and chemical evidence. Saponins 1 and 2 were isolated from G. simonii for the first time.

Saponins are a widespread class of natural compounds found in a lot of plant species. Saponins in general are surface active compounds which give stable foams in water and were shown to effect the plasma membrane of living cells and model membranes by interacting with cholesterol. They are mainly produced by plants and of lower molecular weight (2 kDa). Analysis of saponin compounds, however, can be quite compelling due to such factors as their complexity, amphiphilic structure, lack of strong chromophore, and prone to form unsoluble aggregates in solution which can make purification and quantitation difficult. Underground parts from Gypsophila species (Caryophyllaceae) are an especially rich source of triterpenoid saponins. They are exploited commercially for a variety of purposes including medicines, detergents, adjuvants, and cosmetics. The most common basic structure of sapogenins isolated from Gypsophila genus is mainly gypsogenin but also in fewer amounts gypsogenic acid and quillaic acid. Also, it is shown that aldehyde group at C-4 of sapogenins from Gypsophila is related to cytotoxic activity against human cancer cell lines. Recently, it was shown that Gypsophila saponins amplify the toxicity of saporin and agrostin type I RIPs (ribosome-inactivating proteins) and RIP-based chimeric toxins (Hebestreit, 2006 [28] ). Currently, we showed that nebulosides A and B, novel triterpenoid saponins from Gypsophila arrostii var. nebulosa , a typically Turkish variety, significantly enhanced toxicity of saporin on ECV-304 cell lines. RIPs, generally unable to penetrate the cellular membrane, remove specific adenine residues from the 28S rRNA as part of process that leads to inhibition of protein synthesis. This situation is not based on the damage of cellular membranes by the Gypsophila saponins. Currently, it is known that Gypsophila saponins trigger clathrin-mediated endocytosis of RIPs. This brings up a strong enhancement of toxicity for the naturally membrane-impermeable type I RIPs, making them as toxic as the membrane-permeable type II RIPs, like viscumin and ricin at the same concentration.

A bioassay-guided phytochemical analysis of the triterpene saponins from under ground parts of Gypsophila arrostii var. nebulosa allowed the isolation of two triterpene saponins; nebuloside A, B based on gypsogenin and quillaic acid aglycone. Two new oleanane type triterpenoid saponins (nebuloside A, B) and three known saponins ( 1 – 3 ) were isolated from the root bark of Gypsophila arrostii var. nebulosa . The structures of the two new compounds were elucidated as 3- O -β- d -galactopyranosyl-(1→2)-[β- d -xylopyranosyl-(1→3)]-β- d -glucuronopyranosyl quillaic acid 28- O -β- d -glucopyranosyl-(1→3)-[β- d -xylopyranosyl-(1→3)-β- d -xylopyranosyl-(1→4)]-α- l -rhamnopyranosyl-(1→2)-β- d -fucopyranosyl ester (nebuloside A) and 3- O -β- d -xylopyranosyl-(1→3)-[β- d -galactopyranosyl(1→3)-β- d -galactopyranosyl-(1→2)]-β- d -glucuronopyranosyl gypsogenin 28- O -β- d -glucopyranosyl-(1→3)-[β- d -xylopyranosyl-(1→3)-β- d -xylopyranosyl-(1→4)]-α- l -rhamnopyranosyl-(1→2)-β- d -fucopyranosyl ester (nebuloside B), on the basis of extensive spectral analysis and chemical evidence. Nebuloside A and B showed toxicity enhancing properties on saporin a type-I RIP without causing toxicity by themselves at 15 μg/mL.

Chemical constituents, total phenolic content, total oxidant status, total antioxidant status, lipid hydroperoxides, total free -SH levels, and antimicrobial activity of essential oil obtained from the Ferulago sandrasica (Umbelliferae) were investigated. The essential oil was obtained by hydrodistillation using a Clevenger-type apparatus. The chemical constituents were analyzed by gas chromatography-mass spectrometry. The main components of the essential oil were ocimene (30.5%), carene-δ-3 (27.4%), and pinene (17.8). The antimicrobial activity was tested by a disc diffusion method against E. coli MC 400, E. coli ATCC 25922, E. coli 0157 H7, E. colaecea ATCC 23355, E. feacalis ATCC 19433, P. aeruginosa NRRL B-2679, S. aureus ATCC 25923, B. nischenoformis NRRL B-1001, S. aureus ATCC 33862, B. cereus NRRL B-3711, B. subtilis NRRL B-209, M. luteus NRRL B-1013, L. monocytogenes ATCC 7644, B. subtulis ATCC 6633. © 2013 Copyright Taylor and Francis Group, LLC.

Hederagenin[(3β)-3,23-dihydroxyolean-12-en-28-oic acid], a triterpenoid compound was isolated from a hydro methanolic extract of the underground parts of Gypsophila simonii (Caryophyllaceae). Hederagenin was isolated by HPLC method and molecular mass was determined by the ESI-TOF-MS. The ESI-TOFMS (negative mode) analysis of hederagenin exhibited a pseudo molecular ion peak [M-H]- at m/z 471.30 which is compatible with the molecular formula C30H48O4.

A cytotoxic triterpenoid saponin was isolated from the under-ground parts of Gypsophila pilulifera Boiss.& Heldr. (Caryophyllaceae) naturally grow in the southwestern region of the Turkey. The structures of saponin was elucidated as 3-O-β-d-galactopyranosyl-(1 → 2)-[β-d-xylopyranosyl-(1 → 3)]-β-d-glucuronopyranosyl quillaic acid 28-O-β-d-glucopyranosyl-(1 → 3)-[β-d-xylopyranosyl-(1 → 4]-α-l-rhamnopyranosyl-(1 → 2)-β-d-fucopyranosyl ester on the basis of extensive spectral analysis and chemical evidence. The separated triterpenoid saponin was isolated from Gypsophila pilulifera for the first time. The saponin compound displayed significant cytotoxicity against A549 cell line with IC50 values > 16 μM. © 2012 Elsevier B.V. All rights reserved.

This study established baseline data on lytic anticandidal activities of endemic species Origanum hypericifolium and Salvia potentillifolia naturally distributed in Denizli and its environment. Stream distillation was used to isolate the unfatty polar part and clinical isolated Candida spp. strains were subcultured to sabouraud dextrose agar. Lytic anticandidal activities of unfatty polar parts were evaluated by enzyme-linked calorimetric method against 93 clinical isolates belonging to Candida albicans, C. tropicalis, C. glabrata, C. krusei, C. Kefyr, and C. parapsilosis. As a result, two (2.15%) strains of C. glabrata among tested pathogenic 93 clinical isolates of Candida strains were found to be sensitive to S. potentillifolai. However, each strain of C. albicans and C. tropicalis was found to be sensitive to O. hypericifolium. Results indicated that O. hypericifolium and S. potentillifolia had a potential of being used in food and medicine because of their anticandidal activity.

The chemical compositions, total phenol content, antioxidant and antimicrobial activities with oxidant status of the essential oil from an endemic Turkish species, Origanum hypericifolium, were investigated. Steam distillation (SD) was used to isolate the essential oils, and the chemical analyses were performed by gas chromatography-mass spectrometry (GC-MS). The antimicrobial activity was tested by agar disc diffusion method against Morganella morganii (clinic isolate), Micrococcus flavus (clinic isolate), Micrococcus luteus NRLL B-4375, Proteus vulgaris RSKK 96026, Escherichia coli ATCC 11230, Escherichia coli ATCC 25922, Yersinia enterecolitica RSKK 1501, Staphylococcus aureus ATCC 25923, S. aureus ATCC 25933, S. aureus ATCC 12598, S. aureus (clinic isolate), MRSA 1 (clinic isolate), MRSA 2 (clinic isolate), MRSA 3 (clinic isolate) and MRSA 4 (clinic isolate). The major compounds found in volatiles of O. hypericifolium were p-cymene, carvacrol and γ-terpinene. Results showed that O. hypericifolium has the potential for being used in food and medicine because of its antioxidant and antibacterial activity.

The present study was designed to examine the in vitro free radical scavenging activities of the methanol extracts and essential oil analysis of Salvia cedronella (endemic) and Salvia fruticosa from Turkey. Free radical scavenging activity of methanolic extracts were employed by 2,2’-diphenyl-l-picrylhydrazyl (DPPH). The essential oil of S. cedronella and S. fruticosa were analysed by GC-MS. Totally, 27 components were detected in both hydrodistilled oils, the major components of essential oil of S. cedronella were camphor, ?-thujone and ?-pinene, whereas those of S. fruticosa were 1,8-cineole (eucalyptol), ?-thujone and caryophyllene oxide. © 2010 Taylor & Francis Group, LLC.

The Genus Nepeta L. (Lamiaceae) is represented by 34 species in Turkey, including eighteen endemic species [1, 2]. Nepeta cadmea Boiss. is an endemic species with limited distribution and included in the lower risk and least concern category in the red data book of Turkey [3]. Here we report on the antimicrobial activity of the essential oils from N. cadmea because very little information is available on this endemic species. Table 1 shows the percentages of the main components present in the essential oils isolated from N. cadmea collected in June from Honaz Mountain. The yields of essential oil from N. cadmea on a dry weight basis was 2.1% (v/w). Thirteen components in N. cadmea (97.91%) were identified. The components are listed in order of their elution time on the HP 1 MS column. Among the compounds, nepetalactone (81.6%), caryophyllene (3.71%), and germacrene D (3.25%) were identified as the major components in the essential oil of N. cadmea. The antimicrobial activity of the essential oil measured by the disc diffusion method is given in Table 2. The essential oil isolated from N. cadmea showed antimicrobial activity, but differences in microbial susceptibility were observed. Our findings indicate that the essential oil isolated from N. cadmea has antimicrobial activity and can be used to control microorganisms since this has been used in folk medicine for decades. It will be worth-while to investigate the individual components in antibacterial and antifungal assays.

In present study, antistaphylococcal activity of endemic Salvia chrysophylla Stapf. naturally distributed in Denizli (Turkey) and its vicinity was investigated. The antistaphylococcal activity of the crude extracts was evaluated against two strong microorganisms Staphylococcus aureus and Cowan liyofili. The activity was detected by using broth microdilution methods. When compared with other studies, mic value of our study is further low. The essential oils of endemic Salvia chrysophyla in Denizli was analyzed by GC-MS. The major constituents of the oil of S. chrysophyla were 3-oktanol, a-phellandren-8-ol, camphor and limonene.

WOS: 000454537500021, PubMed: 30600000, This is the first study on production of three dimensional chitinous microcages from ephippial eggs of a microcrustacean, Daphnia longispina (water flea) by keeping the original shape of its chitinous structure. Iron-based magnetic particles were successfully loaded into the chitinous microcages to enhance its heavy metal sorption capacity. The FT-IR, SEM-EDX and TGA analysis proved the purity of chitin and demonstrated that the loading of magnetic particles into the chitinous microcages was achieved. These newly obtained three-dimensional chitin microcages and magnetic particles loaded microcages were tested in Cd(II), Cr(III), Cu(II), Ni(II) and Zn(II) removal from aqueous solutions. Magnetic particles loaded microcages exhibited a better performance in removal of Cd(II), Cu(II) and Ni(II) ions; while unloaded microcages showed a higher affinity for Cr(III) and Zn(II). This study demonstrated that the chitin microcages are suitable carriers for iron-based magnetic particles. Here these new materials were studied only for removal of five heavy metal ions but these promising materials have a potential to be used in various fields., Selcuk University Research Foundation, Turkey [BAP-14201082], This study was funded by Selcuk University Research Foundation, Turkey"(project number: BAP-14201082).