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Given the high prevalence of avian leukosis virus subgroup K (ALV-K) in chickens in China, the positive rate of ALV-K in local chickens in Henan province was investigated, and the genetic region encoding the glycoprotein gp85 of isolates from positive chickens was analyzed. The positive rate of ALV-K in local chickens in Henan was found to be 87.2% (41/47). Phylogenetic analysis of gp85 sequences revealed six clusters that differed in their host range regions (hr1 and hr2) and variable regions (vr1, vr2, and vr3). Evidence of recombination of hr1, hr2, vr1, vr2, and vr3 was observed between the different clusters. The isolate HN23LS02 appears to have obtained its hr1 and hr2 regions from separate lineages via recombination but without having a significant affect on the replication capacity of the virus. [ABSTRACT FROM AUTHOR]

Due to their high porosity, large specific surface area, and structural similarity with the extracellular matrix (ECM), electrospun nanofiber membranes are often endowed with the antibacterial properties for biomedical applications. The purpose of this study was to synthesize nano-structured Sc2O3-MgO by doping Sc3+, calcining at 600 °C, and then loading it onto the PCL/PVP substrates with electrospinning technology with the aim of developing new efficient antibacterial nanofiber membranes for tissue engineering. A scanning electron microscope (SEM) and energy dispersive X-ray spectrometer (EDS) were used to study the morphology of all formulations and analyze the types and contents of the elements, and an X-ray diffraction (XRD), thermogravimetric analysis (TGA), and Fourier transform attenuated total reflection infrared spectroscopy (ATR-FTIR) were used for further analysis. The experimental results showed that the PCL/PVP (SMCV-2.0) nanofibers loaded with 2.0 wt% Sc2O3-MgO were smooth and homogeneous with an average diameter of 252.6 nm; the antibacterial test indicated that a low load concentration of 2.0 wt% Sc2O3-MgO in PCL/PVP (SMCV-2.0) showed a 100% antibacterial rate against Escherichia coli (E. coli). [ABSTRACT FROM AUTHOR]

The influence of uniaxial compressive stress with different orientations to the current channel on the physical and transport properties of the wurtzite GaN heterojunction quantum well is investigated in this work. By using the six-band stress-dependent k × p Hamiltonian, accurate two-dimensional physical pictures are given for the quantized valence subband under the uniaxial compressive stress on the (0001) transport plane. The low-field hole mobility is obtained by the Kubo–Greenwood formula, taking the scattering rates for acoustic phonon, polar optical phonon, and surface roughness into account. Using these methods, the microscopic relationship between the orientation of uniaxial compressive stress and low-field hole mobility is obtained according to the variations of valence subband dispersion and hole effective mass. Results show that for temperatures around and above room temperature, the acoustic phonon scattering is predominant. We find that the mobility gain is mostly contributed from effective mass, and there is an increasing trend under uniaxial compressive stress with all orientations due to the effective mass reduction. For the same stress value, the mobility decreases monotonically as the stress orientation changes from 0° to 90° with respect to the current channel. At room temperature, the calculated low-field hole mobility is 182 cm2/V s under 8 GPa uniaxial compressive stress parallel to the current channel, with the hole density of 5.5 × 1013 cm−2 and the effective electric field of 0.93 MV/cm. [ABSTRACT FROM AUTHOR]

We previously showed that global deletion of the cytochrome P450 epoxygenase Cyp2c44, a major epoxyeicosatrienoic acid (EET) producing enzyme in mice, leads to impaired hepatic insulin signaling resulting in insulin resistance. This finding led us to investigate whether administration of a water soluble EET analog restores insulin signaling in vivo in Cyp2c44(-/-) mice and investigated the underlying mechanisms by which this effect is exerted. Cyp2c44(-/-) mice treated with the analog EET-A for 4 weeks improved fasting glucose and glucose tolerance compared to Cyp2c44(-/-) mice treated with vehicle alone. This beneficial effect was accompanied by enhanced hepatic insulin signaling, decreased expression of gluconeogenic genes and increased expression of glycogenic genes. Mechanistically, we show that insulin-stimulated phosphorylation of insulin receptor β (IRβ) is impaired in primary Cyp2c44(-/-) hepatocytes and this can be restored by cotreatment with EET-A and insulin. Plasma membrane fractionations of livers indicated that EET-A enhances the retention of IRβ in membrane rich fractions, thus potentiating its activation. Altogether, EET analogs ameliorate insulin signaling in a genetic model of hepatic insulin resistance by stabilizing membrane-associated IRβ and potentiating insulin signaling. [ABSTRACT FROM AUTHOR]

This article utilizes two ingestible substrates, pullulan and pectin, to fabricate nanofiber membranes by means of electrospinning technology, and assessed their digestion characteristics in simulated gastrointestinal fluids. The physicochemical properties of pullulan/pectin spinning solution and the effects of electrospinning process parameters on the diameter and morphology of pullulan/pectin nanofibers were investigated. Scanning electron microscope indicates that the best electrospinning process parameters were obtained as spinning voltage: 20 kV, needle-collector distance: 15 cm, propulsion speed: 0.6 mL/h and needle inner diameter: 0.41 mm. Moreover, at a pullulan/pectin volume ratio of 8:2, the nanofiber morphology exhibited optimal characteristics, featuring an average fiber diameter of 369.45 ± 42.41 nm. Fourier transform infrared spectroscopy, X-ray diffraction, and thermal analysis indicates the formation of strong hydrogen bonds between pullulan and pectin. The mechanical performance tests demonstrated that, with a volume ratio of 8:2 for pullulan and pectin, the nanofiber exhibited a tensile strength of 1196.86 ± 51.87 cN and an elongation at break of 80.26 ± 3.6%, indicating favorable capacity for elongation and deformation. Furthermore, the in vitro degradability test indicates that pullulan/pectin nanofibers with a volume ratio of 8:2 were decomposed by specific enzymes in intestinal fluid. Therefore, ingestible pullulan/pectin nanofiber substrates have shown certain development potential in the field of targeted drug or bioactive delivery. • Smooth and uniform PUL/PEC fiber substrates were prepared by electrospinning. • Nanofibers were designed using blend of pullulan and pectin with a mass ratio of 8: 2. • Colon-specific decomposition property was evaluated by an in vitro degradability test. • PUL/PEC nanofibers showed potential as colon delivery systems. [ABSTRACT FROM AUTHOR]

We have recently proposed that the interaction between food components and nanoparticles (NPs) should be considered when evaluating the toxicity of NPs. In the present study, we used THP-1 differentiated macrophages as a model for immune cells and investigated the combined toxicity of low levels of palmitate (PA; 10 or 50 μM) and ZnO NPs. The results showed that PA especially at 50 μM changed the size, Zeta potential and UV–vis spectra of ZnO NPs, indicating a possible coating effect. Up to 32 μg/mL ZnO NPs did not significantly affect mitochondrial activity, intracellular reactive oxygen species (ROS) or release of interleukin 6 (IL-6), but significantly impaired lysosomal function as assessed by neutral red uptake assay and acridine orange staining. The presence of 50 μM PA, but not 10 μM PA, further promoted the toxic effects of ZnO NPs to lysosomes but did not significantly affect other endpoints. In addition, ZnO NPs dose-dependently increased intracellular Zn ions in THP-1 macrophages, which was not significantly affected by PA. Taken together, the results of the present study showed a combined toxicity of low levels of PA and ZnO NPs especially to lysosomes in THP-1 macrophages. [ABSTRACT FROM AUTHOR]

In order to obtain the inorganic efficient antibacterial agents, the means of ion doping and morphology construction in this research are used to enhance the antibacterial property of nano-MgO, which is according to the "oxidative damage mechanism" and "contact mechanism". In this work, the nano-textured Sc2O3-MgO are synthesized by doping Sc3+ in nano-MgO lattice through calcining at 600 °C. When the Sc3+ content reaches 10%, the nanotextures on the powders surface are pretty clearly visible and uniform, and the specific surface area and the oxygen vacancy are ideal, so that the 10% Sc3+-doped powders (SM-10) has the excellent antibacterial property against E. coli and S. aureus (MBC = 0.03 mg/mL). The efficient antibacterial agents in this research have a better antibacterial effect than the 0% Sc3+-doped powders (SM-0, MBC = 0.20 mg/mL) and the commercial nano-MgO (CM, MBC = 0.40 mg/mL), which have application prospects in the field of antibacterial. [ABSTRACT FROM AUTHOR]

The multifunctional polyethylene terephthalate (PET) fabrics were successfully prepared through a dip-coating technology to endow the flame retardant and antibacterial properties of PET fabrics, which are extensively used in many fields. The flame retardant and antibacterial agent was synthesized by a double drop-reverse precipitation method and surface-modified by the mixtures of titanate coupling agents and stearic acid to result in a good compatibility of the hydrophilic nano-Mg(OH)2 and the hydrophobic PET fabrics. The results indicated that the suitable synthesis conditions of nano-Mg(OH)2 are: Mg2+ concentration 1.5 mg/mL, reaction temperature 50 °C and reaction time 50 min, and the optimal modification conditions of nano-Mg(OH)2 are: modifier ratio 5/5, modification temperature 70 °C and modification time 40 min. The flame retardant test and the antibacterial test showed that the multifunctional PET fabrics had excellent flame retardant and antibacterial properties. [ABSTRACT FROM AUTHOR]

Background We examined effects of single-nucleotide variants (SNVs) of IL1RN , the gene encoding the anti-inflammatory interleukin 1 receptor antagonist (IL-1Ra), on the cytokine release syndrome (CRS) and mortality in patients with acute severe respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Methods IL1RN CTA haplotypes formed from 3 SNVs (rs419598, rs315952, rs9005) and the individual SNVs were assessed for association with laboratory markers of inflammation and mortality. We studied 2589 patients hospitalized with SARS-CoV-2 between March 2020 and March 2021. Results Mortality was 15.3% and lower in women than men (13.1% vs 17.3%, P =.0003). Carriers of the CTA-1/2 IL1RN haplotypes exhibited decreased inflammatory markers and increased plasma IL-1Ra. Evaluation of the individual SNVs of the IL1RN, carriers of the rs419598 C/C SNV exhibited significantly reduced inflammatory biomarker levels and numerically lower mortality compared to the C/T-T/T genotype (10.0% vs 17.8%, P =.052) in men, with the most pronounced association observed in male patients ≤74 years old, whose mortality was reduced by 80% (3.1% vs 14.0%, P =.030). Conclusions The IL1RN haplotype CTA and C/C variant of rs419598 are associated with attenuation of the CRS and decreased mortality in men with acute SARS-CoV-2 infection. The data suggest that the IL1RN pathway modulates the severity of coronavirus disease 2019 (COVID-19) via endogenous anti-inflammatory mechanisms. [ABSTRACT FROM AUTHOR]

This study aimed to identify interstitial molecules that were responsible for the deterioration of the esiantimated glomerular filtration rate (eGFR) in diabetic nephropathy (DN). Weighted gene co-expression network analysis (WGCNA) was used to link the tubulointerstitial gene expression profile of DN to eGFR values. The relationship of eGFR with each sub-domain regulator in the network was analyzed with the linear regression model. Gene sets enrichment analysis (GSEA) was applied to detect the molecular changes mostly relating to the essential regulators. Four co-expression modules were found strongly correlating with eGFR values. Genes from these modules were over-represented in fibrosis-related biological processes (extracellular matrix (ECM) organization and cell adhesion) and pathways (integrin signaling and ECM-receptor interaction). Of sub-domains in the gene interaction network, the expression of hypoxia-inducible factor 1A (HIF1A) was most negatively correlated with eGFR (R2 = 0.417, P = 0.026). The positive correlations between HIF1A and its target genes were found, indicating an enhanced transcriptional activity of HIF1A. We also found that HIF1A positively correlated with CCAAT enhancer binding protein delta (CEBPD) (r = 0.731, P = 0.011), an activator of HIF1A transcription. Moreover, GSEA showed that samples with high HIF1A expression were enriched with fibrosis associated signaling, like ECM-receptor interaction and cell adhesion. Intriguingly, vascular epithelial growth factor A (VEGFA) expression decreased while HIF1A increased (R2 = 0.733, P = 0.001), suggesting VEGFA loss may exacerbate hypoxia and stimulate HIF1A induction. The present study suggested that interstitial HIF1A may be involved in renal interstitial fibrosis in DN. Mechanisms of eGFR decrease in DN. In DN, the loss of tubular epithelial cells induces the decrease of VEGFA levels, which exaggerates tubulointerstitial hypoxia through diminishing the peritubular capillary density. The interstitial hypoxia promotes HIF1A transcriptional expression, via increasing the levels of HIF1A transcriptional activator (CEBPD). HIF1A causes eGFR deterioration via epithelial to mesenchymal transition and fibrosis. Unlabelled Image [ABSTRACT FROM AUTHOR]

Antibacterial and active packaging materials have gained significant research attention in response to the growing interest in food packaging. In this investigation, we developed hydrogel packaging materials with antibacterial and antioxidant properties by incorporating chitooligosaccharide (COS) and fish skin gelatin (FSG) nanofiber membranes, which readily absorbed water and exhibited swelling characteristics. The nanofiber membranes were fabricated by electrospinning technology, embedding COS within FSG, and subsequently crosslinked through the Maillard reaction facilitated by the addition of glucose. The behavior of conductivity, viscosity, and surface tension in the spinning solutions was analyzed to understand their variation patterns. Scanning electron microscopy (SEM) results revealed that the crosslinked COS/FSG nanofiber membranes possessed a uniform yet disordered fiber structure, with the diameter of the nanofibers increasing as the COS content increased. Remarkably, when the COS content reached 25 %, the COS/FSG nanofiber membranes (CF-C-25) exhibited a suitable fiber diameter of 437.16 ± 63.20 nm. Furthermore, the thermal crosslinking process involving glucose supplementation enhanced the hydrophobicity of CF-C-25. Upon hydration, the CF-H-25 hydrogel displayed a distinctive porous structure, exhibiting a remarkable swelling rate of 954 %. Notably, the inclusion of COS significantly augmented the antibacterial and antioxidant properties of the hydrogel-based nanofiber membranes. CF-H-25 demonstrated an impressive growth inhibition of 90.56 ± 5.91 % against E. coli , coupled with excellent antioxidant capabilities. In continuation, we performed a comprehensive analysis of the total colony count, pH, TVB-N, and TBA of crucian carp. The CF-H-25 hydrogel proved highly effective in extending the shelf life of crucian carp by 2–4 days, suggesting its potential application as an edible membrane for aquatic product packaging. [Display omitted] • Nanofibers composed of COS/FSG were synthetized via the electrospinning technique. • Hydrogel was prepared by water absorption swelling of nanofibers after Maillard reaction. • COS/FSG nanofiber-based hydrogel was fabricated for food packaging. • COS/FSG nanofiber-based hydrogel have significant antibacterial and antioxidant properties. [ABSTRACT FROM AUTHOR]

It was recently shown that exposure to ZnO nanoparticles (NPs) could induce endoplasmic reticulum (ER) stress both in vivo and in vitro, but the role of ER stress in ZnO NP induced toxicity remains unclear. Because macrophages are sensitive to ER stress, we hypothesized that stressing macrophages with ER stress inducer could enhance the toxicity of ZnO NPs. In this study, the effects of ER stress inducer thapsigargin (TG) on the toxicity of ZnO NPs to THP-1 macrophages were investigated. The results showed that TG enhanced ZnO NP induced cytotoxicity as revealed by water soluble tetrazolium-1 (WST-1) and neutral red uptake assays, but not lactate dehydrogenase (LDH) assay. ZnO NPs dose-dependently enhanced the accumulation of intracellular Zn ions without the induction of reactive oxygen species (ROS), and the presence of TG did not significantly affect these effects. In the co-culture, exposure of THP-1 macrophages in the upper chamber to ZnO NPs and TG significantly reduced the viability of human umbilical vein endothelial cells (HUVECs) in the lower chamber, but the release of tumor necrosis factor α (TNFα) was not induced. In summary, our data showed that stressing THP-1 macrophages with TG enhanced the cytotoxicity of ZnO NPs to macrophages and macrophage-endothelial co-cultures. [ABSTRACT FROM AUTHOR]

Simple Summary: Lung adenocarcinoma (LADC) is the pathological type with the highest morbidity and mortality among lung cancers. Although achievements in new therapeutic approaches have been developed, chemotherapy is still the most widely choice for control of LADC. However, the increasing drug resistance becomes the major challenge, so the development of the novel and efficient chemotherapeutic drug is still urgent. Elaiophylin, a new type of autophagy inhibitor, has been shown to possess unique anti-cancer activity. In this study, we have deeply investigated the therapeutic effect of elaiophylin on LADC and found elaiophylin exerts its anti-cancer effect though inhibiting mitophagy and oxidative stress and targeting SIRT1/Nrf2 signaling. This innovative and comprehensive research may provide the possibility for the development of novel chemotherapy drug for LADC. Lung adenocarcinoma (LADC), the most common type of lung cancer, is still one of the most aggressive and rapidly fatal tumor types, even though achievements in new therapeutic approaches have been developed. Elaiophylin as a C2 symmetrically glycosylated 16 macrolides has been reported to be a late-stage autophagy inhibitor with a potent anti-tumor effect on various cancers. This study investigated the anti-tumor effect of elaiophylin on human LADC for the first time in in vitro and in vivo models. The in vitro study in LADC A549 cells showed that elaiophylin significantly inhibited cell viability and induced cell apoptosis through the suppression of mitophagy and induction of cellular and mitochondrial oxidative stress. Proteomic analysis and molecular docking assay implicated that SIRT1 was likely the direct target of elaiophylin in A549 cells. Further mechanistic study verified that elaiophylin reduced Nrf2 deacetylation, expression, and transcriptional activity as well as cytoplasm translocation by downregulating SIRT1 expression and deacetylase activity. Additionally, SIRT1/Nrf2 activation could attenuate elaiophylin-induced mitophagy inhibition and oxidative stress. The in vivo study in the A549-xenograft mice model showed that the anti-tumor effect of elaiophylin was accompanied by the decreased expressions of SIRT1, Nrf2, Parkin, and PINK1. Thus, the present study reports that elaiophylin has potent anti-tumor properties in LADC, which effect is likely mediated through suppressing the SIRT1/Nrf2 signaling. In conclusion, elaiophylin may be a novel drug candidate for LADC and SIRT1 may be a new therapeutic target for such devastating malignancy. [ABSTRACT FROM AUTHOR]

In order to fabricate antibacterial nanofiber membranes for tissue engineering field, the oxidative damage of magnesium hydroxide nanoparticles (Mg(OH) 2 NPs) against the model system of Escherichia coli (E. coli) was researched by measuring the oxidative stress response, oxidative stress products and glucose metabolism. The toxicity of Mg(OH) 2 NPs to a normal biological system was evaluated in vivo through the acute oral toxicity, blood physiological and serum biochemical markers and acute skin irritation, indicating that the Mg(OH) 2 NPs exhibited great biosafety. The Mg(OH) 2 NPs with antibacterial property and biosafety were loaded on PCL/PVP nanofiber substrates through electrospinning technology to fabricate the Mg(OH) 2 /PCL/PVP nanofiber membranes. The results of characterization and antibacterial test showed that the Mg(OH) 2 /PCL/PVP nanofiber membranes with 8.0 wt% Mg(OH) 2 NPs present excellent antibacterial rate of 100% against E. coli that the Mg(OH) 2 NPs can be physically and evenly loaded on nanofiber substrates. • Oxidative damage of Mg(OH) 2 NPs against E. coli was evaluated. • Biosafety of Mg(OH) 2 NPs was evaluated in vivo. • Mg(OH) 2 /PCL/PVP nanofiber membranes were fabricated for the first time. • Mg(OH) 2 NPs were loaded on nanofiber substrates by electrospinning method. [ABSTRACT FROM AUTHOR]

Lung adenocarcinoma (LADC) is the most prevalent lung cancer sub-type, and targeted therapy developed in recent years has made progress in its treatment. Erdafitinib, a potent and selective pan-FGFR tyrosine kinase inhibitor, has been confirmed to be effective for the treatment of LADC; however, the molecular mechanism responsible for this effect is unclear. The in vitro study showed that erdafitinib exhibited an outstanding anti-cancer activity in human LADC cell line A549 by inducing S-phase cell-cycle arrest and cell apoptosis. The mechanistic study based on the transcriptomic data revealed that erdafitinib exerted its anti-cancer effect by affecting the cell cycle-related pathway, and CDK2 was the regulatory target of this drug. In addition, CDK2 overexpression significantly attenuated the anti-cancer effect of erdafitinib by affecting the transcriptional activity and expression of E2F1, as well as the expression of CDK1. The in vivo study showed that erdafitinib presented an obvious anti-cancer effect in the A549 xenograft mice model, which was accompanied by the reduced expression of CDK2. Thus, this study demonstrates the anti-cancer effect of erdafitinib against LADC for the first time based on in vitro and in vivo models, whose activity is achieved by targeting CDK2 and regulating downstream E2F1-CDK1 signaling. This study may be helpful for expanding the clinical application of erdafitinib in treating LADC. [ABSTRACT FROM AUTHOR]

The external alternating magnetic field (AMF) strategy is the most promising research topic to solve the bottleneck of catalyst activity enhancement. However, the mechanism between the arrangement of the spin electrons under the AMF and the catalytic activity enhancement for water splitting is still unclear. Herein, we synthesize Fe 3 O 4 @CNTs heterostructure as a research model and resolve the mechanism by system theoretical analysis and in-situ Raman characterization. The multiple orbital interactions of σ(d z 2, p z , s) and π(d xz - p x , d yz - p y) promote the hexa-coordinated Fe oct for both the reductive HER and oxidative OER processes. The AMF excites the transition from low to high spin configurations of the Fe oct sites, which accelerates charge transfer of unpaired d electrons and optimizes adsorption and desorption interactions to intermediates during the reaction processes, resulting in a significant enhancement of the electrocatalytic activity (HER: 32 mV at 10 mA cm−2, OER: 179 mV at 100 mA cm−2). [Display omitted] • Density functional theory calculations reveal the high catalytic activity of hexa-coordinated iron sites. • Constructing heterostructures and applying external magnetic fields jointly influence material activity. • Mechanism for enhancing the activity of high-spin state electron orbitals through external magnetic field regulation. • In-situ Raman real-time monitoring of highly active FeOOH species during the reaction. [ABSTRACT FROM AUTHOR]

[Display omitted] • By optimizing the internal electronic structure and exposing more active sites. • The synergistic effect of heterogeneous atoms and oxygen defects. • The unique hexagonal morphology is more favorable to the exposure of active sites. • The highly active and stable bifunctional cobalt oxide-based catalysts. Undesirable conductivity and insufficient electrocatalytic activity are the current bottlenecks of cobalt tetroxide from achieving efficient water electrolysis. Herein, by optimizing the internal electronic structure and the controlled design of the morphology, we design a unique hexagonal Mn-Co 3 O 4 -200 nanostructure that exhibits superior water electrolysis performance with low overpotentials and excellent long-term durability under strongly alkaline conditions, outperforming many other previously reported cobalt oxide-based compounds. Theoretical combined with characterization analysis indicates that introduced Mn atoms induce an increase in the high spin-orbital occupancy of Co2+ to optimize free energy of the OOH*→O 2 step, which excites the catalytic activity of the Co sites, resulting in a substantial increase in the catalytic activity of the Mn-Co 3 O 4. Furthermore, we briefly summarize the catalytic activity variation pattern of several catalysts with different microscopy morphologies and innovatively propose a more intuitive means to evaluate their exposed active areas. Compared with other morphologies, the special hexagonal morphology with abundant pores and large effectively exposed catalytic surface area can promote the exposure rate of active centers, mass transfer of electrolytes and diffusion of hydrogen and oxygen in the electrocatalytic process, thus accelerating the rate of electrocatalytic reactions. [ABSTRACT FROM AUTHOR]

In the presented work, vibrational behavior related to a curved nanopipe which is under supersonic airflow and conveying fluid flow, is examined. To model the size-dependent nanopipe, the Quasi-2D hybrid type of nonlocal strain gradient theory (QHNSGT) is presented. Formulations are obtained by means of Hamilton's principle for bi-directional functionally graded (Bi-FG) nanopipe. Also, formulations are solved by means of the generalized differential quadrature method (GDQM). By taking into account that the fluid flow is infinite, incompressible, uniform flow, Newtonian, laminar, as well, as viscous, and with the help of the Navier-Stokes equation, the fluid-structure interaction is obtained. A quasi-2D hybrid type of higher-order shear deformation theory is employed to introduce the displacement fields. The verification section shows that the results of this paper are very near to the results of the published article in the literature. One of the important findings of the current research is critical values of the Mach number could have increased with the aid of increasing the rigidity of edges and decreasing fluid flow velocity. Another marvelous output is that opening angle and airflow stability have an indirect relation, and increasing the opening angle provides an expansion in the unstable area. • Vibrational behavior related to a curved nanopipe under supersonic airflow. • To model the size-dependent nanopipeand QHNSGT is presented. • By considering infinite fluid flow, incompressible, uniform flow, Newtonian, laminar, and viscous, fluid-structure interaction is obtained. • A quasi-2D hybrid type of higher-order shear deformation theory is employed to introduce the displacement fields. [ABSTRACT FROM AUTHOR]

An explicit approximation for surface potential as a function of terminal voltages is developed for partially depleted polysilicon thin-film transistors. The resulting model has taken both Gaussian deep states and exponential tail states into account, which provides a complete modeling for surface potential. Furthermore, a drain current model based on terms of surface potential is proposed. Surface potential and drain current calculations are successfully verified by comparisons with numerical and experimental ones. [ABSTRACT FROM PUBLISHER]

It was recently shown that ZnO nanoparticles (NPs) could induce endoplasmic reticulum (ER) stress in human umbilical vein endothelial cells (HUVECs). If ER stress is associated the toxicity of ZnO NPs, the presence of ER stress inducer thapsigargin (TG) should alter the response of HUVECs to ZnO NP exposure. In this study, we addressed this issue by assessing cytotoxicity, oxidative stress and inflammatory responses in ZnO NP exposed HUVECs with or without the presence of TG. Moreover, TiO2NPs were used to compare the effects. Exposure to 32 μg/mL ZnO NPs (p < 0.05), but not TiO2NPs (p > 0.05), significantly induced cytotoxicity as assessed by WST-1 and neutral red uptake assay, as well as intracellular ROS. ZnO NPs dose-dependently increased the accumulation of intracellular Zn ions, and ZnSO4induced similar cytotoxic effects as ZnO NPs, which indicated a role of Zn ions. The release of inflammatory proteins tumor necrosis factor α (TNFα) and interleukin-6 (IL-6) or the adhesion of THP-1 monocytes to HUVECs was not significantly affected by ZnO or TiO2NP exposure (p > 0.05). The presence of 250 nM TG significantly induced cytotoxicity, release of IL-6 and THP-1 monocyte adhesion (p < 0.01), but did not significantly affect intracellular ROS or release of TNFα (p > 0.05). ANOVA analysis indicated no interaction between exposure to ZnO NPs and the presence of TG on almost all the endpoints (p > 0.05) except neutral red uptake assay (p < 0.01). We concluded ER stress is probably not associated with ZnO NP exposure induced oxidative stress and inflammatory responses in HUVECs. [ABSTRACT FROM PUBLISHER]

Nanoparticles (NPs) are increasingly used in food, and the toxicity of NPs following oral exposure should be carefully assessed to ensure the safety. Indeed, a number of studies have shown that oral exposure to NPs, especially solid NPs, may induce toxicological responses both in vivo and in vitro. However, most of the toxicological studies only used NPs for oral exposure, and the potential interaction between NPs and food components in real life was ignored. In this review, we summarized the relevant studies and suggested that the interaction between NPs and food components may exist by that 1) NPs directly affect nutrients absorption through disruption of microvilli or alteration in expression of nutrient transporter genes; 2) food components directly affect NP absorption through physico-chemical modification; 3) the presence of food components affect oxidative stress induced by NPs. All of these interactions may eventually enhance or reduce the toxicological responses induced by NPs following oral exposure. Studies only using NPs for oral exposure may therefore lead to misinterpretation and underestimation/overestimation of toxicity of NPs, and it is necessary to assess the synergistic effects of NPs in a complex system when considering the safety of NPs used in food. [ABSTRACT FROM AUTHOR]

The article discusses the study on the major subtypes of obesity such as the general and the abdominal obesity which are known to be associated with chronic diabetes complications, like cardiovascular diseases and diabetic retinopathy. Topics discussed include the diabetic kidney disease (DKD), type 2 diabetes (T2D) patients assess through DXA, and the parameters of general obesity such as body mass index (BMI), total body fat percentage(TBF), and fat mass index (FMI).