1. The global distribution and diversity of protein vaccine candidate antigens in the highly virulent Streptococcus pnuemoniae serotype 1
- Author
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Cornick, Jennifer E., Tastan Bishop, Özlem, Yalcin, Feyruz, Kiran, Anmol M., Kumwenda, Benjamin, Chaguza, Chrispin, Govindpershad, Shanil, Ousmane, Sani, Senghore, Madikay, du Plessis, Mignon, Pluschke, Gerd, Ebruke, Chinelo, McGee, Lesley, Sigaùque, Beutel, Collard, Jean-Marc, Bentley, Stephen D., Kadioglu, Aras, Antonio, Martin, von Gottberg, Anne, French, Neil, Klugman, Keith P., Heyderman, Robert S., Alderson, Mark, Everett, Dean B., PAGe consortium, Malawi Liverpool Wellcome Trust Clinical Research Programme (MLW), Liverpool School of Tropical Medicine (LSTM)-University of Liverpool-Wellcome Trust-University of Malawi, University of Liverpool, Rhodes University, Grahamstown, The Wellcome Trust Sanger Institute [Cambridge], National Institute for Communicable Diseases [Johannesburg] (NICD), University of the Witwatersrand [Johannesburg] (WITS), Centre de Recherche Médicale et Sanitaire (Niamey, Niger) (CERMES), Réseau International des Instituts Pasteur (RIIP), Medical Research Council Unit The Gambia (MRC), University of Warwick [Coventry], Swiss Tropical and Public Health Institute [Basel], Centers for Disease Control and Prevention [Atlanta] (CDC), Centers for Disease Control and Prevention, Centro de Investigação em Saúde de Manhiça [Maputo, Mozambique] (CISM), London School of Hygiene and Tropical Medicine (LSHTM), Emory University [Atlanta, GA], PATH [Seattle], This work supported by the Bill and Melinda Gates Foundation (Grant No. OPP1023440), Wellcome Trust (Award No. 084679/Z/08/Z), National Research Foundation of South Africa (Grant No. 93690) and the NIH Common Fund Award (H3A Bionet) (Grant No. U41HG006941)., and A full list of PAGe members can be found at http://www.pagegenomes.org/page/consortium
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Models, Molecular ,Vaccination Coverage ,Antigenic diversity ,MESH: Geography ,MESH: Vaccines, Subunit ,MESH: Amino Acid Sequence ,MESH: Global Health ,MESH: Africa ,MESH: Virulence ,Global Health ,Structural diversity ,Pneumococcal Vaccines ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,MESH: Pneumococcal Infections ,Variant ,MESH: Bacterial Proteins ,Geography ,Virulence ,MESH: Asia ,Research Support, Non-U.S. Gov't ,MESH: Vaccination Coverage ,Protein modelling ,Antigenic Variation ,Europe ,Infectious Diseases ,Streptococcus pneumoniae ,Vaccines, Subunit ,Molecular Medicine ,MESH: Antigenic Variation ,PCV ,MESH: Models, Molecular ,MESH: Streptococcus pneumoniae ,MESH: Pneumococcal Vaccines ,Asia ,Antigenic profiling ,Pneumococcal disease ,Serogroup ,Article ,Pneumococcal Infections ,Bacterial Proteins ,Research Support, N.I.H., Extramural ,Immunology and Microbiology(all) ,Journal Article ,Humans ,Multi-valent ,Amino Acid Sequence ,Alleles ,Antigens, Bacterial ,MESH: Humans ,MESH: Alleles ,Public Health, Environmental and Occupational Health ,MESH: Serogroup ,MESH: South America ,South America ,veterinary(all) ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Africa ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,MESH: Europe ,MESH: Antigens, Bacterial - Abstract
International audience; Serotype 1 is one of the most common causes of pneumococcal disease worldwide. Pneumococcal protein vaccines are currently being developed as an alternate intervention strategy to pneumococcal conjugate vaccines. Pre-requisites for an efficacious pneumococcal protein vaccine are universal presence and minimal variation of the target antigen in the pneumococcal population, and the capability to induce a robust human immune response. We used in silico analysis to assess the prevalence of seven protein vaccine candidates (CbpA, PcpA, PhtD, PspA, SP0148, SP1912, SP2108) among 445 serotype 1 pneumococci from 26 different countries, across four continents. CbpA (76%), PspA (68%), PhtD (28%), PcpA (11%) were not universally encoded in the study population, and would not provide full coverage against serotype 1. PcpA was widely present in the European (82%), but not in the African (2%) population. A multi-valent vaccine incorporating CbpA, PcpA, PhtD and PspA was predicted to provide coverage against 86% of the global population. SP0148, SP1912 and SP2108 were universally encoded and we further assessed their predicted amino acid, antigenic and structural variation. Multiple allelic variants of these proteins were identified, different allelic variants dominated in different continents; the observed variation was predicted to impact the antigenicity and structure of two SP0148 variants, one SP1912 variant and four SP2108 variants, however these variants were each only present in a small fraction of the global population (
- Published
- 2017