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This paper presents the isolation of kenaf bast fiber to produce cellulose nanofiber (CNF) using chemical-physical treatment (e.g. delignification, bleaching and ultrasonication). Raw bast fiber wa...

The issue of food safety is a major concern in the oil palm industry as it will affect national income. 3-MCPD and GE are contaminants formed during the refining of palm oil, mainly in the deodorization step. Palm oil was reported to contain one of the highest levels of these contaminants amongst all vegetable oils. Both 3-MCPD and GE are characterized as a possible risk to human health. This review aimed to provide a comprehensive summary of the 3-MCPD and GE precursors and mitigation strategies to minimize the 3-MCPD and GE formation. Therefore, the oil palm industry should address these issues and find ways to comply with food laws, acts, regulations, and standards enforced by local and international authorities.

The immense demand of plastics worldwide has created a substantial pollution problem that appears not to have an end. New governmental policies and customers preferences towards environmental friendly materials require continued research on biodegradable materials derived from biological resource. In this work, residual palm oil (RPO) recovered from palm oil mill waste effluent was mixed with algae oil (AO) to produce rigid foam polyurethanes (bio-RFPU) at different ratios (AO/RPO) 10/90, 20/80, 30/70, 40/60 and 50/50 by one pot epoxidation and ring opening followed by polymerization with Methylene diphenyl diisocyanate (MDI). Prior to polymerization, significant improvement of 49% was found in the hydroxyl number of the RPO(AO) polyol when AO content was increased from 10 to 50%. Similarly, the homogeneous structure of the cell and the thermal stability of the final bio-RFPU was increased in the samples with 50% AO. Different characterization techniques such as scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), dynamic mechanical analysis (DMA) and compression/flexural tests were conducted, and the results presented. The thermal degradation profile of the samples containing 40 and 50% of AO was found to be similar to RFPU with fire retardant and insulation properties. The compressive strength and the biodegradability of the bio-RFPU increased 60% when AO was increased between samples with 10 and 50% AO. Therefore, the developed bio-RFPUs is expected to have great potential as core materials in industrial applications such as sandwich panels as well as in other insulation material.

This study was carried out to compare the antioxidant and nutritional properties of coconut (Cocos nucifera L.) sap with other natural sources of sugar such as sugar palm (Borassus flabellifer) and sugarcane (Saccharum officinarum L.). Coconut sap and juice from sugar palm and sugarcane were analyzed for proximate composition, pH and total soluble solid (TSS), color, sugar profile, vitamin profile, antioxidant properties (total phenolic contents, DPPH, FRAP, and ABTS), and mineral content. The results indicated that coconut sap possesses high DPPH (23.42%), FRAP (2.09 mM/ml), and ABTS (21.85%) compared with the juices. Coconut sap also had high vitamin C (116.19 µg/ml) and ash (0.27%) contents, especially in potassium (960.87 mg/L) and sodium (183.21 mg/L) which also indicating high content of minerals. These properties showed that coconut sap could be served as a potential healthier sugar source compared with sugar palm and sugarcane juices., Coconut sap could be served as a potential healthier sugar source compared with sugar palm and sugarcane juices as it carries more minerals, antioxidants, and vitamins.

Cassava (Manihot esculenta Crantz) is one of the main sources of carbohydrate and it is a useful plant in Malaysia. Its root is used to produce various foods such as cassava chips, while the other parts are potentially to produce feeds and other useful products. However, details observation in the proximate composition of each part of the cassava plant is important in utilizing it as an animal feed, especially for the ruminants. Hence, this research was conducted to evaluate the proximate composition of each part in the cassava plant and characterized its functional groups using Fourier-transform infrared spectroscopy (FTIR) and the thermal properties using thermogravimetric analyzer (TGA). The results obtained showed that the portions of fresh cassava plants namely flesh, stem, peel, discarded tuber and the leaf were recorded at 50.06%, 31.01%, 10.63%, 6.92% and 1.49% (w/w), respectively. Proximate analysis showed that the leaf has potential as animal feed as it contains significant high in crude protein (28.02±0.10%), crude fat (5.63± 0.12%) and the gross energy value of 4824.3 g/cal. Even though the leaf has a higher cyanide content (0.02 mg/kg) than other parts, it is still in acceptable range as an ingredient in animal feed. For animal feed that focuses on high protein and gross energy, the leaf has potential in feed ingredients. Meanwhile, cassava flesh also has potential as an animal feed since it has low crude fiber (2.11±0.03%) but high in carbohydrate (92.66±1.88%) and gross energy content (4223.9 cal/g). FTIR spectra showed that there were different functional groups present in the samples. From the TGA data, it showed that the major components in samples were cellulose which started to decompose rapidly at maximum degradation rate temperature of 315-400°C, especially for flesh and discarded tuber. Different parts of cassava plants are expected to help the agriculture industry in producing alternative animal feed at the same time minimizing the impact of waste generated in the environment.

In the effort to produce renewable and biodegradable polymers, more studies are being undertaken to explore environmentally friendly sources to replace petroleum-based sources. The oil palm industry is not only the biggest vegetable-oil producer from crops but also one the biggest producers of residual oil that cannot be used for edible purposes due to its low quality. In this paper the development of biopolymers from residual palm oil, residual palm oil with 10% jatropha oil, and residual palm oil with 10% algae oil as additives were explored. Polyols from the different oils were prepared by epoxydation with peroxyacetic acid and alcoholysis under the same conditions and further reacted with poly isocyanate to form polyurethanes. Epoxidized oils, polyols and polyurethanes were analyzed by different techniques such as TGA, DSC, DMA, FTIR and H-NMR. Overall, although the IV of algae oil is slightly higher than that of jatropha oil, the usage of algae oil as additive into the residual palm oil was shown to significantly increase the hard segments and thermal stability of the bio polyurethane compared to the polymer with jatropha oil. Furthermore, when algae oil was mixed with the residual palm oil, it was possible to identify phosphate groups in the polyol which might enhance the fire-retardant properties of the final biopolymer.

Palm kernel is the edible seed of the oil palm fruit obtained during the palm oil milling process. For efficient processing and storage, the moisture content of palm kernel must be reduced to an optimal level by drying. This study aimed to see how drying influenced the physical structure and physicochemical properties of palm kernel and oil. Before and after drying, the free fatty acid (FFA), color, fatty acid composition, Fourier transform infrared, thermal properties and structure of palm kernel were investigated.Results show that drying significantly (P 0.05) reduced FFA and color of palm kernel oil. Drying also significantly affected (P 0.05) composition of some fatty acids in palm kernel oil such as capric acid, lauric acid, myristic acid, palmitic acid and oleic acid. However, drying did not affect palm kernel and palm kernel oil functional groups and thermal properties. After drying, structural damage of palm kernel was observed.Based on these findings, the quality of kernel oil may be maintained after drying, and it can even be improved based on lower FFA content. © 2022 Society of Chemical Industry.

The objective of this study was to evaluate the effect of different initial carbon to nitrogen (C/N) ratios on the organic matter degradation during active co-composting of oil palm empty fruit bunch (OPEFB) and palm oil mill effluent (POME) anaerobic sludge. The initial C/N ratio was varied from 25:1, 35:1 and 45:1. Co-composting was conducted by periodic addition of sludge to maintain the moisture content and enrich the compost product. The organic matter (OM), carbon to nitrogen profile and compost maturity index were analysed. The results showed that the initial C/N ratio of 35:1 was the best initial C/N ratio. In addition, the C/N ratio of 35:1 gave the best OM degradation. The appropriate amount of initial C/N ratio coupled with the correct composting process parameters such as daily mixing, suitable pH and moisture content improved the organic matter degradation. It reduced the composting time from 40-60 days to 30 days.

Sterilisation in palm oil mills is considered a pre-treatment process as it affects stripping efficiency and oil quality. Although sterilisation technology has been well-established in the palm oil milling industry, the roles and principles of sterilisation, particularly related to the chemical changes in fruits and stalks occurring during the process, have been rarely reported. The review begins with the background literature on the biochemical properties of the FFBs, such as the compositions of binding carbohydrates and the phenomena of natural fruit detachment. Followed by the harvesting practice to understand the type of FFBs supplied to the industry. In addition, a comparison of the well-established conventional and alternative sterilisation technologies and sterilisation functions is critically reviewed and assessed. Establishing the current sterilisation process initiatives to address the natural fruit’s separation more efficiently in palm oil mills is important. Particularly visualise sterilisation as a breakup of specific binding carbohydrates that leads to strippability. It will provide a further understanding of the sterilisation mechanism, which would benefit the palm oil miller in optimising the processing of fresh fruit bunches. The information provided in this review is necessary to mitigate the percentage of unstripped bunches and reduce the oil losses and ultimately enhance the oil extraction rate.

Crude palm oil (CPO) is the main product of oil palm processing and is obtained through a series of unit operations. In a palm oil mill, the main unit operations involve applying saturated steam in the steriliser and digester. The high temperature and water content used will induce thermal hydrolysis, leading to accumulation of free fatty acids (FFAs), the levels of which are considered a major quality index of CPO. A detailed study of the thermal hydrolysis of CPO at different temperatures and water contents in a closed-vessel system was carried out. The results show that there was an increase in FFA accumulation as temperature and initial water content increased. A kinetic model of the hydrolysis was constructed taking into account heat and mass transfer phenomena. The important parameters of the model were the reaction frequency factor (k0T) and the power factor of water fraction (n), which were successfully estimated by gPROMS ModelBuilder at 2.55 × 10–6 (m3/kmol)0.85 min−1 and 0.62, respectively. A sensitivity analysis revealed that the simulated profiles (i.e. FFA content and vapour mass flux, W ˙ W V ) were highly sensitive to these parameters. The model can be a useful tool in the further redesign and quality improvement of the industrial of CPO extraction processes.

Investigation on viscoelastic behaviour of oil palm fibres was reported through experimental and finite element modelling study. The experimental work through tensile stress relaxation and cyclic tests revealed time-dependent behaviour and damage within the oil palm fibres. From the former test results, stresses of fresh fibres reduced more than the dried ones after 1 second relaxation, whereas increasing damage was observed under larger deformations from the latter test results. Finite element modelling results using Prony series viscoelastic model with damage function only agreed with small deformation test, whereas Parallel Rheological Framework viscoelastic model agreed with large deformation test. The former model can be used for biodegradation study, which does not involve large deformation, whereas the latter model is suitable for biocomposites study under large deformation.

This paper demonstrates the potential use of toy-bricks as the building block of a mechanical tensile testing instrument for the mechanical characterisation of natural fibres. A table-top tensile testing instrument was developed using LEGO parts (Mindstorms EV3 and Technics) and a 2 kg capacity load cell, whereas deformation modes were programmed in an open source programming language. Experimental work was conducted on oil palm fibres under different tensile modes (i.e. constant deformation, triple-twisted-tension and deformation-relaxation modes), which showed anisotropic-viscoelastic behaviour, and microstructural damages due to deformation.

This research aimed to improve the stability of Chlorella-Alginate Beads (CABs) by zeolite molecular sieves 13X. Dissolution time of synthesized Zeolite-Algal-Alginate Beads (ZABs) in a chelating agent revealed a significant improvement on the beads stability (78.5 ± 0.5 min) compared to the control beads (51.5 ± 0.5 min) under the optimum conditions of zeolite/alginate (1.5:1), pH 5 and 2% of beads. Monitoring cell growth during 5 days of incubation showed good biocompatibility of zeolite 13X. Scanning electron microscopy (SEM) indicated rough surface and spherical shapes of ZABs. Energy dispersive X-ray spectroscopy (EDX) and Fourier transform infrared spectroscopy (FTIR) of ZABs confirmed the presence of zeolite 13X within the matrix. The zeta potential value of ZABs indicated that the beads were relatively stable. The findings of this research showed that zeolite molecular sieves 13X have the potential to improve the stability of algal-alginate beads compared to common beads.

The influence of biomass cellulosic content on biochar nanopore structure and adsorption capacity in aqueous phase was scarcely reported. Commercial cellulose (100% cellulose), oil palm frond (39.5% cellulose), and palm kernel shell (20.5% cellulose) were pyrolyzed AT 630 °C, characterized and tested for the adsorption of iodine and organic contaminants. The external surface area and average pore size increased with cellulosic content, where commercial cellulose formed biochar with external surface area of 95.4 m2/g and average pore size of 4.1 nm. The biochar from commercial cellulose had the largest adsorption capacities: 371.40 mg/g for iodine, 86.7 mg/L for tannic acid, 17.89 mg/g for COD and 60.35 mg/g for colour, while biochar from palm kernel shell had the least adsorption capacities. The cellulosic content reflected the differences in biochar nanopore structure and adsorption capacities, signifying the suitability of highly cellulosic biomass for producing biochar to effectively treat wastewater.

Cassava (Manihot esculenta Crantz) is the third-largest carbohydrate food source in the tropical region after rice and corn. Its flesh is used to produce foods, while the other parts of the plant are not fully exploited. This research aimed to evaluatetheproximate composition of the cassava plant (Rayong 90 variety). Six different parts namely leaf, petiole, young stem, tuber, cortex and periderm of white variety cassava plants were dried and powdered to determine their proximate composition (protein, carbohydrate, fat, crude fiber, ash, moisture). The results show that the leaf is the most nutritional compared to other parts. Leaf had 43.80% carbohydrate, 23.87% protein, 13.66% crude fiber, 6.53% ash, 6.00 % crude fat and 5.00% moisture content. The crude protein for other parts were very low, ranging from 2.54% to 10.20%. The white cassava variety plant was very high in carbohydrates, ranging from 30.48% to 86.77% of dry matter. The mean percentage of crude fat found within the range from 0% to 6.00%. From the results, it can be concluded that a leaf of white cassava variety can be used to develop a potential high carbohydrate and protein ruminant feed because it has appreciable levels of the nutrient.

A study on the effect of the physical properties and moisture sorption isotherm of palm kernels constitutes the critical criteria in evaluating the drying performance. The drying was evaluated as a function of moisture content (MC) in the range of 0.31&ndash, 0.02 kg/kg (d.b.). Whereas, the equilibrium moisture content (EMC) of palm kernels (whole kernel and ground kernel) was determined experimentally using the standard gravimetric method at different temperatures (50 &deg, C to 80 &deg, C), over a range of relative humidity (RH) from 10% to 81%. Palm kernel length, width, and thickness decrease from 16.08 &plusmn, 2.09 mm to 14.17 &plusmn, 2.30 mm, 12.06 &plusmn, 1.40 mm to 11.24 &plusmn, 1.08 mm, and 10.01 &plusmn, 1.27 mm to 9.18 &plusmn, 1.04 mm, respectively, when MC decreased. Bulk density, surface area, and specific surface area decreased as the MC decreased, while porosity and true density were increased. EMC of palm kernels (whole kernel and ground kernel) decreased with an increase in temperature at constant RH. Modified Oswin and modified Halsey models were found to be the best for predicting desorption moisture isotherms for whole and ground palm kernel, respectively. Therefore, the study of the effect of drying on physical aspects as well as moisture sorption isotherms is important to further analyze the drying performance of Tenera palm kernel (e.g., equipment design and energy requirement).

Orange-fleshed sweet potato (OFSP) offers many health benefits and can be processed into different food forms including as a healthy drinking juice. However, the OFSP juice requires exploration on the juice properties, especially its nutritional elements before it can be marketed as a healthy drink. Hence, this study aimed to address the detailed nutritional composition of the OFSP juice via analyses of proximate compositions, amino acids, mineral, carotenoids, and vitamins. Findings showed OFSP juice is proven to be high in beta-carotene with a value of 4916.06 μg/L which was almost 100 times the value of beta-carotene in orange juice, and also rich in vitamins, certain amino acids and minerals. Since these phytochemicals can aid in the reduction of anti-mutagenic, immune-enhancers, cancer, and free radical scavengers, this study has proven the potential of OFSP to be processed into a healthy juice based on its nutritional properties.

The interest in utilizing algae for wastewater treatment has been increased due to many advantages. Algae-wastewater treatment system offers a cost-efficient and environmentally friendly alternative to conventional treatment processes such as electrocoagulation and flocculation. In this biosystem, algae can assimilate nutrients in the wastewater for their growth and simultaneously capture the carbon dioxide from the atmosphere during photosynthesis resulting in a decrease in the greenhouse gaseousness. Furthermore, the algal biomass obtained from the treatment process could be further converted to produce high value-added products. However, the recovery of free suspended algae from the treated effluent is one of the most important challenges during the treatment process as the current methods such as centrifugation and filtration are faced with the high cost. Immobilization of algae is a suitable approach to overcome the harvesting issue. However, there are some drawbacks with the common immobilization carriers such as alginate and polyacrylamide related to low stability and toxicity, respectively. Hence, it is necessary to apply a new carrier without the mentioned problems. One of the carriers that can be a suitable candidate for the immobilization is zeolite. To date, various types of zeolite have been used for the immobilization of cells of bacteria and yeast. If there is any possibility to apply them for the immobilization of algae, it needs to be considered in further studies. This article reviews cell immobilization technique, biomass immobilization onto zeolites, and algal immobilization with their applications. Furthermore, the potential application of zeolite as an ideal carrier for algal immobilization has been discussed.

The main objective of this work was to investigate the effects of the controlled periodic addition of anaerobic sludge during composting to increase amount of microbial DNA, which appears to be correlated to soluble sugar content which may relate to rate of lignocellulosic degradation. In this study, the composting of pressed-shredded oil palm empty fruit bunch with the periodic addition of palm oil mill effluent anaerobic sludge for moisture control in a newly designed in-vessel type composter was carried out. A control experiment was also conducted over the same period but with the periodic addition of water for moisture control instead of the anaerobic sludge. The lignocellulosic composition and the reducing sugar content were determined via fibre analysis and the spectrophotometric method respectively. The bacterial profile throughout the composting process was quantified by using qPCR. The growth of bacteria reached its peak at 48°C and the degradation of lignocellulose was highest during the thermophilic stage. The highest content of reducing sugar coincided with the highest degradation rate of lignocellulose and the highest DNA copy number during the thermophilic stage. Under the controlled experimental condition of increasing the microbial community, the composting was accelerated to 2.07% OM degradation per day compared to the water addition control at 0.60% OM per day.

Crude palm oil (CPO) and palm-pressed mesocarp fibre oil (PPMFO) consist of minor components such as Vitamin E, phytosterols, squalene, and carotene that play numerous health function in human. However, limitations are present in current separation processes to separate or enrich minor components from plant sources. In this study, interest is focusing on the enrichment efficiency of the minor components from CPO and PPMFO through proposed sequential adsorption-desorption strategy. The strategy started with the adsorption-desorption properties evaluation of six adsorbents. Both Diaion HP20 and Sepabeads SP850 showed better adsorption-desorption properties than silica gel, Florisil, Diaion HP2MG and Amberlite XAD-7HP. Diaion HP20 was selected as the suitable adsorbent to perform a series of selective desorption process using three different solvents: methanol, IPA, and n-hexane in Soxhlet extraction. Vitamin E, phytosterols, and squalene from CPO were obtained in the 1st fraction using methanol solution and their concentration increased from the initial concentration with the enrichment factor (EF) of 3.4, 3.9, and 1.8, respectively, which slightly higher than those minor components obtained from PPMFO, 1.2, 1.8, and 1.4, respectively. The carotene from both CPO and PPMFO was enriched in the 3rd fraction by using n-hexane solution with an enrichment factor of 1.1 and 1.5, respectively. In conclusion, the obtained result revealed the efficiency of the proposed sequential adsorption-desorption strategy to enrich the minor components from CPO and PPMFO.

In recent years, downstream bioprocessing industries are venturing into less tedious, simple, and high-efficiency separation by implementing advanced purification and extraction methods. This review discusses the separation of proteins, with the main focus on amylase as an enzyme from agricultural waste using conventional and advanced techniques of extraction and purification via a liquid biphasic system (LBS). In comparison to other methods, such as membrane extraction, precipitation, ultrasonication, and chromatography, the LBS stands out as an efficient, cost-effective, and adaptable developing method for protein recovery. The two-phase separation method can be water-soluble polymers, or polymer and salt, or alcohol and salt, which is a simpler and lower-cost method that can be used at a larger purification scale. The comparison of different approaches in LBS for amylase purification from agricultural waste is also included. Current technology has evolved from a simple LBS into microwave-assisted LBS, liquid biphasic flotation (LBF), thermoseparation (TMP), three-phase partitioning (TPP), ultrasound-assisted LBS, and electrically assisted LBS. pH, time, temperature, and concentration are some of the significant research parameters considered in the review of advanced techniques.

Enzyme immobilization via covalent binding provides a strong interaction between enzyme and support material. In this study, the effect of different coupling agents (spacer arms and ligands) in cyclodextrin glucanotransferase (CGTase) immobilization on bleached kenaf microfiber as a support matrix was investigated. The immobilized CGTase properties such as storage stability, thermal stability and reusability were evaluated. Immobilized CGTases on microfiber resulted in 0.162–0.24 U/mg-fiber when 55.6 U/mL of CGTase activity was initially added during the immobilization. The highest storage stability (60 °C) was shown by CGTase that was immobilized with ethylenediamine and o-phthalaldehyde, whereby 60% of its activity remained after 15 days. Its high stability was also confirmed by the lowest deactivation constant, kd that was obtained at 25 °C (0.0161 day−1) and 60 °C (0.0361 day−1). The CGTase immobilized using ethylenediamine and glutaraldehyde has shown the best retention of enzyme activity up to 72.72% after 12 cycles of batch reaction. The results indicate that kenaf microfiber has potential to be applied as a support for enzyme immobilization and its enzymatic properties were affected by the coupling agents.

Cellulose nanofiber (CNF) from kenaf bast fiber has potential to be used in enzyme immobilization. This study discloses the preparation of CNF, immobilization of cyclodextrin glucanotranferase (CGTase) on CNF via chemical coupling and its application using ultrafiltration membrane. Morphological analysis shows the diameter distribution of CNF in nano-order scale (

The use of plastics and composite materials as the base structure for a floating solar photovoltaic system commonly known as floatovoltaic system can cause negative impact to the environment especially the aquatic life and human. Additive materials are infused to form the plastics by which giving adverse effect to our metabolism. Despite the fact that the plastics are durable, to create and recycle it will produce toxic materials of many kinds in addition to the higher expenses for floatovoltaic installation. The unique approach of floatovoltaic is further discussed withstanding the continuous constraint of limited and suitable land for solar PV farms. This paper presents field analysis of the development of coconut-trunk as the base structure for a floatovoltaic system with regards to the environmental impact. A framework based on the density and buoyancy of the coconut trunk material is considered for the design

A large volume of oil palm empty fruit bunch (EFB) is generated as waste feedstock around the globe. This abundant waste containing 0.75% oil on average could be a promising feedstock for biodiesel production if oil recovery could be accomplished in an economically-viable and environmentally-friendly manner. To achieve that, a new method called High Pressure Water Spray (HPWS) system was introduced and performed by spraying pressurized water (500 psi) at 30 °C, 60 °C, and 90 °C and combination of water-steam at 120 °C and 150 °C onto the surface of the oil palm empty fruit bunches (EFB). The results obtained indicated that, the highest oil removal yield of 94.41 ± 0.02 wt% was obtained at 150 °C. Moreover, bioprospection of biodiesel properties based on fatty acid methyl ester (FAME) profile revealed that the biodiesels produced from the fresh crude palm oil and residual oil were comparable and were in accordance with international standards. In addition to that, the HPWS process led to an enhanced quality of the remaining lignocellulosic materials for conversion into other value added bio-products such as ligno-ethanol by decreasing lignin content and increasing cellulose content. In view of environmental impact assessment, the HPWS system showed favorable impacts on all the end-point damage categories especially in resources damage category. Moreover, economic assessment showed that the recovered CPO could be generated at a low price of USD 0.41 vs. USD 0.66 for CPO. Overall, this process could drastically increase the market value of an abundant type of waste in many parts of the world, i.e., EFB leading to the generation of additional wealth for the palm oil industry.

Background Coconut sugar has a caramel color with a taste like brown sugar. It is commonly used as natural sweetener. However, coconut sugar has been produced from coconut sap using a traditional method that involves heating the sap at high temperature (>100 °C) in an open pan for a long period (3-5 h). This conventional method results in an over-cooked sugar, which leads to quality deterioration in terms of both its physical and chemical properties. The current study aimed to investigate the processing of coconut sap into sugar syrup using alternative processing techniques such as rotary vacuum evaporation (RE) and microwave evaporation (ME), comparing them with open-heat evaporation (OHE) technique. Results Coconut sugar syrup produced by rotary evaporation at 60 °C and 250 mbar vacuum (RE-60) required the shortest production time (12.2 min) and the lowest processing temperature (54.8 °C) when compared with ME (13 min and 103.2 °C) and OHE (46.8 min and 101.6 °C). It also had a light brownish color with a higher L* value (35.17) than the ME (29.84) and OHE (23.84) methods. It was found to contain higher amounts of monosaccharides (fructose and glucose) and lower amounts of disaccharides (sucrose). Furthermore, the amount of energy required for RE-60 (0.35 kWh) was much less than for OHE (0.83 kWh). Conclusion This study provided an alternative processing method for the sugar processing industry to produce coconut sugar using the rotary evaporation method at 60 °C under 250 mbar vacuum with better physicochemical qualities, shorter processing time, and minimum input energy. © 2020 Society of Chemical Industry.

The heterogeneous lignocellulosic contents of oil palm empty fruit bunch (OPEFB) fiber requires the use of chemical or biological pre-treatment for further value-added applications. An effective biological pre-treatment was preferred since it is mild, low energy, substrate specific and will not generate any inhibitory chemicals that interfere enzymatic activities and fermentation process. The biological pre-treatment of OPEFB fibers by local isolate Pycnoporus sanguineus was investigated in this work to improve its biodegradability through laccase production via solid-state fermentation approach. The effects of physical parameters (temperature, inducer concentrations, substrate loading) were explored to obtain maximum laccase production by P. sanguines. Results showed that the use of extractive-free OPEFB fiber at 30 °C supplemented with 4 mM of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 10 g of OPEFB loading yielded laccase production that was 2.1 fold higher compared to raw OPEFB. The changes of chemical and surface structures of raw, chemicals and laccase treated OPEFB were reported from Fourier-transform infrared spectroscopy (FTIR), thermogravimetric (TGA) and scanning electron microscopy (SEM) analyses. The simulated techno-economic analysis suggested economic feasibility of a batch bio-refinery for laccase production in an industrial scale feed at a maximum rate of 50 tons OPEFB/batch. From the simulated baseline study, the cost price forecast for laccase production was 14.26 US$/kg owing to the capital (65.87%) and raw materials costs (31.68%). This study reveals the potential of P. sanguineus to be used not only in biorefinery industry but also in biopulping, biobleaching (in textile and paper industries) and degrading phenolic waste (dye, colorant, pesticide waste).

In order to meet the growing demand for adsorbents to treat wastewater effectively, there has been increased interest in using sustainable biomass feedstocks. In this present study, the dermal tissue of oil palm frond was pyrolyzed with superheated steam at 500 °C to produce nanoporous biochar as bioadsorbent. The effect of operating conditions was investigated to understand the adsorption mechanism and to enhance the adsorption of phenol and tannic acid. The biochar had a microporous structure with a Brunauer-Emmett-Teller surface area of 422 m2/g containing low polar groups. The adsorption capacity of 62.89 mg/g for phenol and 67.41 mg/g for tannic acid were obtained using 3 g/L biochar dosage after 8 h of treatment at solution pH of 6.5 and temperature of 45 °C. The Freundlich model had the best fit to the isotherm data of phenol (R2 of 0.9863), while the Langmuir model best elucidated the isotherm data of tannic acid (R2 of 0.9632). These indicated that the biochar-phenol interface was associated with a heterogeneous multilayer sorption mechanism, while the biochar-tannic acid interface had a nonspecific monolayer sorption mechanism. The residual concentration of 26.3 mg/L phenol and 23.1 mg/L tannic acid was achieved when treated from 260 mg/L three times consecutively with 1 g/L biochar dosage, compared to a reduction to 72.3 mg/L phenol and 69.9 mg/L tannic acid using 3 g/L biochar dosage in a single treatment. The biochar exhibited effective adsorption of phenol and tannic acid, making it possible to treat effluents that contain varieties of phenolic compounds.

Xylitol is an extracellular sugar alcohol produced by the biological conversion of xylose through the fermentation process. The present study investigates the reaction kinetics of xylitol fermentation by considering the effect of substrate and product concentration on the microbial growth rate. A 3.5-L batch fermentation produced xylitol at different xylose concentrations and agitation speeds. The experimental data showed that the xylose concentration limit was less than 100 g/L and that increasing the xylose concentration reduced the xylitol and cell yields. The optimum agitation speed was 400 rpm with a kLa value of 32.6 h−1. The experimental data were used to estimate the unknown parameters with gPROMS software. The Monod model was modified to include the inhibitory effect of the substrate and the limitation effect of dissolved oxygen on cell growth.

This research mainly focuses on developing a natural cellulose nanofibre (CNF) from kenaf bast fibre and its potential for enzyme immobilization support. CNF was isolated by using a combination between chemical and mechanical treatments such as alkaline process and high-intensity ultrasonication process to increase the efficiency of hemicellulose and lignin removal, and to reduce its size into nano-order. The morphological study was carried out by using scanning electron microscope (SEM), indicating most of CNF diameter in range of 50-90 nm was obtained. The result of chemical analysis shows that cellulose content of raw bast fibre, bleached pulp fibre and CNF are 66.4 %, 83.7 % and 90.0 %, respectively. By decreasing the size of cellulose fibre, it increases the number of (O–H) group on the surface that plays as important role in enzyme immobilization. Covalent immobilization of cyclodextrin glucanotransferase (CGTase) onto CNF support resulted in about 95.0 % of protein loading with 69.48 % of enzyme activity, indicating high immobilization yield of enzyme. The enzymatic reaction of immobilized CGTase was able to produce more than 40 % yield of α-CD. Reusability profile of immobilized CGTase resulted in more than 60 % of retained activity up to 7 cycles. Therefore, the CNF is highly potential to be applied as enzyme immobilization support.

Advances in biochar production and modification have extended the applications of biochar to wastewater treatment. However, not all feedstocks produced porous biochar at a moderate temperature suitable for wastewater treatment. In this study, biochar was produced from oil palm frond using steam pyrolysis at 500 °C and pulverized to granular and micro-fine particles. Both biochar particles were characterized and applied as adsorbents for treating final discharge of palm oil mill effluent. The effluent was also filtered and treated to examine the effect of suspended solids on adsorption capacity. The biochar had Brunauer-Emmett-Teller surface area of 406.6 m2 g−1. Pulverization eliminated the residual macropores in granular biochar, created new external surface area, and exposed constricted nanopores, which resulted in increasing the surface area to 457.7 m2 g−1. The adsorption capacity decreased from 24.6 to 6.1 mg g−1 for chemical oxygen demand and 49.0 to 10.9 Pt–Co g−1 for color by increasing the dosage of micro-fine biochar from 5 to 30 g L−1. The total suspended solids affected the adsorption capacity of granular biochar by blocking residual macropores that provide access to adsorption sites in micropores and mesopores. At 30 g L−1, the micro-fine biochar exhibited an effective reduction of chemical oxygen demand from 224 to 41.6 mg g−1 and color from 344 to 15 Pt–Co g−1 making the wastewater suitable for reuse in palm oil mills and safe for discharge into the aquatic environment.