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Cruciferous sprout is a new form of vegetable product rich in bioactive compounds, especially glucosinolates. Previous studies have focused on increasing the accumulation of glucosinolates in cruciferous sprouts by applying different chemical regulators, with a particular focus on their contribution to nutritional quality and health benefits. Nevertheless, the effects of melatonin and UV-B irradiation on glucosinolate biosynthesis remain unclear. In this study, it was found that changes in melatonin concentrations significantly affected the contents of individual as well as total aliphatic and indolic glucosinolates. The 5 μmol · L−1 melatonin was decided as the optimum concentration that could increase the content of beneficial glucosinolates including glucoraphanin and 4-methoxy glucobrassicin in Chinese kale sprouts. Notably, the enhancement of glucosinolate accumulation by melatonin treatment could be further amplified by UV-B irradiation. Furthermore, our results showed that R2R3-MYB transcription factor BoaMYB28 and BoaMYB51, which are central regulators of aliphatic and indolic glucosinolate biosynthesis respectively, were both involved in the regulation of glucosinolate biosynthesis by melatonin and UV-B irradiation. Additionally, the expression of glucosinolate biosynthetic genes, including BoaCYP79F1, BoaCYP83A1, BoaSUR1, BoaUGT74B1, BoaCYP79B2, BoaCYP79B3, and BoaCYP83B1 participated in the formation of core structures and BoaFMOGS-OX5, BoaAOP2, BoaCYP81F2, and BoaIGMT1 involved in the side-chain modification of aliphatic and indolic glucosinolate, was regulated by melatonin or UV-B irradiation. Taken together, these findings provide a potential strategy for improving the nutritional quality and resistance of Chinese kale sprouts.

Abstract. Background:. Psychological stress has been reported to be a potential risk factor for hypertension among females, but it remains unclear whether spousal chronic stress levels alter the risk of hypertension among women. We examined the associations between stress within the family and hypertension among married women. Methods:. Reproductive-aged women who were planning for pregnancy and their husbands were recruited from the National Free Pre-pregnancy Checkup Projects (NFPCP) across 31 provinces in China in 2016 and 2017. Perceived stress of wives or husbands was measured with a 5-point Likert-type scale, and assessed from three domains: work/life-related stress, economic stress, and overall stress. Multivariable-adjusted logistic regression models were used to assess the associations between stress status and the prevalence of hypertension. Results:. Of 10,027,644 couples, 261,098 (2.60%) women had hypertension. The results showed that higher stress levels among themselves or their husbands were associated with a higher prevalence of hypertension in women (Pfor trend

Background: Currently, awareness about platelet count (PC) and its consequences for perinatal outcome have increased, but there is little reliable evidence on fecundability. Methods: Based on the National Free Pre-conception Check-up Projects supported by the Chinese government, 5,524,886 couples met the inclusion criteria and were included in this cohort study. Cox regression models were adopted to estimate fecundability ratios (FRs) and their 95% confidence intervals (CIs) for pre-pregnancy PC quintiles. Restricted cubic splines were used to flexibly model and visualize the relationship of PC with FRs. Microsoft SQL server and R software were used for data management and analysis. Results: The median of pre-pregnancy PC among women was 221.00 × 109/L. The first (223.06 × 109/L) were associated with the risk of reduced female fecundability (P for non-linearity < 0.01). Conclusion: PC is associated with female fecundability. Further classification of PC levels may deepen our understanding of the early warnings and significance of female fecundability.

Abstract To address the issues of low accuracy and slow response speed in tea disease classification and identification, an improved YOLOv7 lightweight model was proposed in this study. The lightweight MobileNeXt was used as the backbone network to reduce computational load and enhance efficiency. Additionally, a dual-layer routing attention mechanism was introduced to enhance the model’s ability to capture crucial details and textures in disease images, thereby improving accuracy. The SIoU loss function was employed to mitigate missed and erroneous judgments, resulting in improved recognition amidst complex image backgrounds.The revised model achieved precision, recall, and average precision of 93.5%, 89.9%, and 92.1%, respectively, representing increases of 4.5%, 1.9%, and 2.6% over the original model. Furthermore, the model’s volum was reduced by 24.69M, the total param was reduced by 12.88M, while detection speed was increased by 24.41 frames per second. This enhanced model efficiently and accurately identifies tea disease types, offering the benefits of lower parameter count and faster detection, thereby establishing a robust foundation for tea disease monitoring and prevention efforts.

Abstract Background Folic acid supplementation is recommended for reducing the risk of birth defects. We aimed to assess the protective association of periconception folic acid supplements with birth defects in real-world setting. Methods This prospective, population-based cohort study utilized national preconception registered data of married Chinese couples planning a pregnancy within 6 months between 2010 and 2012 in Mainland China. Participated women are freely provided folic acid starting 3 months before conception till 3 months after conception. Birth defects were self-reported at 42 days postpartumn followup. R software (v4.0.2) was applied for statistical analyses. Results Complete data of 567,547 couples with pregnancy outcomes and folic acid supplementation were extracted for final analysis. A total of 74.7% women were with folic acid supplementation, and 599 birth defects were self-reported. The odd of birth defects was lower among women taking folic acid compared to their counterparts not taking (0.102% vs 0.116%, P

Post-fermented Pu-erh tea (PFPT) is a microbial fermented tea characterized by unique sensory attributes and multiple health benefits. Aspergillus niger is the dominant fungus involved in the fermentation process and plays a significant role in imparting the distinct characteristics of PFPT. To investigate the role of Aspergillus niger in the fermentation of Pu-erh tea, this study inoculated unsterilized sun-dried green tea with Aspergillus niger isolated from Pu-erh tea to enhance the fermentation process. Metabolites and microbial communities in sun-dried green tea (CK), fortified fermented tea (TF), and naturally fermented tea (NF) were analyzed using non-targeted metabolomics, 16S rDNA, and internal transcribed spacer sequencing. Non-targeted metabolomics revealed that Aspergillus niger significantly altered the metabolite profile of the tea samples, identifying a total of 200 different metabolites, with 95 showing significant increases and 105 significant decreases, predominantly enriched in metabolic pathways associated with amino acid biosynthesis and degradation. High-throughput sequencing revealed that although the relative abundance of the fungal community remained largely unchanged, the inoculation of Aspergillus niger significantly increased the abundance of Bacillales and Pseudomonas within the bacterial community, thereby influencing the dynamic balance of the microbial ecosystem. Collectively, the inoculation of Aspergillus niger altered the composition of the microbial community and metabolic activities, resulting in changes to the content of amino acid-dominated metabolites, thereby enhancing the flavor profile and overall quality of Pu-erh tea. These findings provide important insights for optimizing the production processes of Pu-erh tea and the application of microorganisms in other fermented foods.

Understanding the correlation between soil chemical properties and tea quality is essential for the comprehensive management of ancient tea gardens. However, the specific links between these factors in ancient tea gardens remain underexplored. This study analyzes the soil chemical properties of four distinct research regions in Nanhua County to explore their effects on key chemical components in ancient tea garden teas, providing a scientific basis for improving the quality of ancient tea garden teas through soil management. Employing high performance liquid chromatography (HPLC) and inductively coupled plasma mass spectrometry (ICP-MS), the chemical components of tea and the chemical properties of the soil were meticulously quantified. Following these measurements, the integrated fertility index (IFI) and the potential ecological risk index (PERI) were evaluated and correlation analysis was conducted. The results revealed that ancient tea garden tea quality is closely linked to soil chemical properties. Soil’s total nitrogen (TN), total sulfur (TS), and available potassium (AK) negatively correlate with tea’s catechin gallate (CG) component and AK also with polyphenols. Most other soil properties show positive correlations with tea components. The research also evaluated soil heavy metals’ IFI and PERI. IFI varied significantly among regions. Hg’s high pollution index indicates ecological risks; Cd in Xiaochun (XC) region poses a moderate risk. PERI suggests moderate risk for XC and Banpo (BP), with other areas classified as low risk. Implementing reasonable fertilization and soil amelioration measures to enhance soil fertility and ensure adequate supply of key nutrients will improve the quality of ancient tea gardens. At the same time, soil management measures should effectively control heavy metal pollution to ensure the quality and safety of tea products. Insights from this study are crucial for optimizing soil management in ancient tea gardens, potentially improving tea quality and sustainability.

Objective: To investigate the relationship between ambient fine particulate matter (PM2.5) exposure and fecundability. Methods: This study included 751,270 female residents from Henan Province who participated in the National Free Pre-conception Check-up Projects during 2015–2017. Ambient cycle-specific PM2.5 exposure was assessed at the county level for each participant using satellite-based PM2.5 concentration data at 1-km resolution. Cox proportional hazards models with time-varying exposure were used to estimate the association between fecundability and PM2.5 exposure, adjusted for potential individual risk factors. Results: During the study period, 568,713 participants were pregnant, monthly mean PM2.5 concentrations varied from 25.5 to 114.0 µg/m3 across study areas. For each 10 µg/m3 increase in cycle-specific PM2.5, the hazard ratio for fecundability was 0.951 (95 % confidence interval: 0.950–0.953). The association was more pronounced in women who were older, with urban household registration, history of pregnancy, higher body mass index (BMI), hypertension, without exposure to tobacco, or whose male partners were older, with higher BMI, or hypertension. Conclusion: In this population-based prospective cohort, ambient cycle-specific PM2.5 exposure was associated with reduced fecundability. These findings may support the adverse implications of severe air pollution on reproductive health.

Anthocyanin accumulation is acknowledged as a phenotypic indicator of phosphate (Pi) starvation. However, negative regulators of this process and their molecular mechanisms remain largely unexplored. In this study, we demonstrate that WRKY33 acts as a negative regulator of phosphorus-status-dependent anthocyanin biosynthesis. WRKY33 regulates the expression of the gene encoding dihydroflavonol 4-reductase (DFR), a rate-limiting enzyme in anthocyanin production, both directly and indirectly. WRKY33 binds directly to the DFR promoter to repress its expression and also interferes with the MBW complex through interacting with PAP1 to indirectly influence DFR transcriptional activation. Under −Pi conditions, PHR1 interacts with WRKY33, and the protein level of WRKY33 decreases; the repression of DFR expression by WRKY33 is thus attenuated, leading to anthocyanin accumulation in Arabidopsis. Further genetic and biochemical assays suggest that PHR1 is also involved in regulating factors that affect WRKY33 protein turnover. Taken together, our findings reveal that Pi starvation represses WRKY33, a repressor of anthocyanin biosynthesis, to finely tune anthocyanin biosynthesis. This “double-negative logic” regulation of phosphorus-status-dependent anthocyanin biosynthesis is required for the maintenance of plant metabolic homeostasis during acclimation to Pi starvation.

Abstract Background The postponement of parenthood is a global public health issue that has received attention of many public health experts. However, few studies have investigated the postponement in marriage age, marriage and conception interval, and pregnancy age in terms of demographic and regional heterogenicities. Methods This is a cross-sectional, registry-based study, and a total of 13 894 601 nulliparous couples who participated in the National Free Pre-Pregnancy Check-ups Project and became pregnant during 2013–2019 were included. We calculated annual percentage change and forest plots for marriage age, marriage and conception interval, and pregnancy age. Results Late marriage (marriage age ≥ 35 years), long marriage and conception interval (marriage and conception interval ≥ 2 years), and advanced pregnancy (pregnancy age ≥ 35 years) increased from 1.20%, 22.01%, and 1.88% in 2013 to 1.69%, 32.75%, and 2.79% in 2019, respectively. The corresponding annual percentage changes were 6.55%, 8.44%, and 8.17%. Participants without higher education had a higher annual percentage change, but comparable prevalence for long marriage and conception interval with participants with higher education. Participants residing in second- or new first-tier cities, and the northeast of China who had a higher prevalence of parenthood postponement also had higher corresponding annual percentage changes. Conclusions Structural postponement of parenthood with demographic and regional heterogenicities was observed among Chinese nulliparous couples with planned pregnancies during 2013–2019. Inclusive and comprehensive parenting support should be developed and implemented in mainland China to minimize the negative health effects arising from the postponement, especially for couples without higher education and living in new first/second-tier cities or the northeast China.

China’s Yunnan Province, known for its tea plantations, faces significant challenges in smart pest management due to its ecologically intricate environment. To enable the intelligent monitoring of pests within tea plantations, this study introduces a novel image recognition algorithm, designated as YOLOv8n-WSE-pest. Taking into account the pest image data collected from organic tea gardens in Yunnan, this study utilizes the YOLOv8n network as a foundation and optimizes the original loss function using WIoU-v3 to achieve dynamic gradient allocation and improve the prediction accuracy. The addition of the Spatial and Channel Reconstruction Convolution structure in the Backbone layer reduces redundant spatial and channel features, thereby reducing the model’s complexity. The integration of the Efficient Multi-Scale Attention Module with Cross-Spatial Learning enables the model to have more flexible global attention. The research results demonstrate that compared to the original YOLOv8n model, the improved YOLOv8n-WSE-pest model shows increases in the precision, recall, mAP50, and F1 score by 3.12%, 5.65%, 2.18%, and 4.43%, respectively. In external validation, the mAP of the model outperforms other deep learning networks such as Faster-RCNN, SSD, and the original YOLOv8n, with improvements of 14.34%, 8.85%, and 2.18%, respectively. In summary, the intelligent tea garden pest identification model proposed in this study excels at precise the detection of key pests in tea plantations, enhancing the efficiency and accuracy of pest management through the application of advanced techniques in applied science.

IntroductionIn order to solve the problem of precise identification and counting of tea pests, this study has proposed a novel tea pest identification method based on improved YOLOv7 network.MethodsThis method used MPDIoU to optimize the original loss function, which improved the convergence speed of the model and simplifies the calculation process. Replace part of the network structure of the original model using Spatial and Channel reconstruction Convolution to reduce redundant features, lower the complexity of the model, and reduce computational costs. The Vision Transformer with Bi-Level Routing Attention has been incorporated to enhance the flexibility of model calculation allocation and content perception.ResultsThe experimental results revealed that the enhanced YOLOv7 model significantly boosted Precision, Recall, F1, and mAP by 5.68%, 5.14%, 5.41%, and 2.58% respectively, compared to the original YOLOv7. Furthermore, when compared to deep learning networks such as SSD, Faster Region-based Convolutional Neural Network (RCNN), and the original YOLOv7, this method proves to be superior while being externally validated. It exhibited a noticeable improvement in the FPS rates, with increments of 5.75 HZ, 34.42 HZ, and 25.44 HZ respectively. Moreover, the mAP for actual detection experiences significant enhancements, with respective increases of 2.49%, 12.26%, and 7.26%. Additionally, the parameter size is reduced by 1.39 G relative to the original model.DiscussionThe improved model can not only identify and count tea pests efficiently and accurately, but also has the characteristics of high recognition rate, low parameters and high detection speed. It is of great significance to achieve realize the intelligent and precise prevention and control of tea pests.

Accurate grasp of chicken body temperature can effectively improve the success rate of caged chicken breeding, by monitoring the number of open-mouthed chickens as a percentage of the total number of chickens and can directly determine whether the chicken body temperature is appropriate. There is no relevant solution to this requirement at present, so this paper proposes a joint feature target detection algorithm based on YOLOv5 to detect the opening and closing state of the chicken mouth. The algorithm improves the YOLOv5 network in the following ways: 1) The improved ResC module is used to reconstruct the backbone network of YOLOv5, which diversifies feature scales and enhances the ability of target feature extraction; 2) Integrate the Transformer module with the four-layer feature pyramid to expand the range of feature fusion and improve the accuracy of feature extraction; 3) The joint feature verification(JFV) module is designed to improve the detection accuracy of small targets by adopting the idea of joint verification of small targets and large targets. Finally, the improved network is used to detect the opening and closing state of the chicken beak on the test set, which is derived from the actual cage chicken breeding environment. The results show that the average accuracy (mAP) of the improved RJ-YOLOv5 algorithm is 85.6%, and the detection accuracy is 7.1% higher than the YOLOv5 algorithm; The video detection frame rate reaches 69 FPS, which can meet the requirements of real-time monitoring of chicken farms.

Variations in the concentration of glucoraphanin (GRA) and glucoerucin (GER), as well as the corresponding breakdown products, isothiocyanates (ITCs) and nitriles, were investigated during the growth of broccoli sprouts. The concentrations of GRA and GER decreased sharply from 33.66 µmol/g to 11.48 µmol/g and 12.98 µmol/g to 8.23 µmol/g, respectively, after seed germination. From the third to the seventh day, both GRA and GER were maintained as relatively stable. The highest concentrations of sulforaphane (17.16 µmol/g) and erucin (12.26 µmol/g) were observed on the first day. Hereafter, the concentrations of nitrile hydrolyzed from GRA or GER were higher than those of the corresponding ITCs. Moreover, the ratio of sulforaphane to sulforaphane nitrile decreased from 1.35 to 0.164 from 1 d to 5 d, with a similar trend exhibited for erucin/erucin nitrile after 2 d. RNA-seq analysis showed that BolMYB28 and BolCYP83A1, involved in aliphatic glucosinolate (GSL) biosynthesis, remained largely unexpressed until the third day. In contrast, the genes operating within the GSL-myrosinase hydrolysis pathway were highly expressed right from the beginning, with their expression levels increasing significantly after the third day. Additionally, we identified two BolESPs and six BolNSPs that might play important roles in promoting the production of nitriles during the development of broccoli sprouts.

To investigate the variation in flavonoids content in ancient tree sun–dried green tea under abiotic stress environmental conditions, this study determined the flavonoids content in ancient tree sun−dried green tea and analyzed its correlation with corresponding factors such as the age, height, altitude, and soil composition of the tree. This study uses two machine−learning models, Least Absolute Shrinkage and Selection Operator (LASSO) regression and Cox regression, to build a predictive model based on the selection of effective variables. During the process, bootstrap was used to expand the dataset for single−factor and multi−factor comparative analyses, as well as for model validation, and the goodness−of−fit was assessed using the Akaike information criterion (AIC). The results showed that pH, total potassium, nitrate nitrogen, available phosphorus, hydrolytic nitrogen, and ammonium nitrogen have a high accuracy in predicting the flavonoids content of this model and have a synergistic effect on the production of flavonoids in the ancient tree tea. In this prediction model, when the flavonoids content was >6‰, the area under the curve of the training set and validation set were 0.8121 and 0.792 and, when the flavonoids content was >9‰, the area under the curve of the training set and validation set were 0.877 and 0.889, demonstrating good consistency. Compared to modeling with all significantly correlated factors (p < 0.05), the AIC decreased by 32.534%. Simultaneously, a visualization system for predicting flavonoids content in ancient tree sun−dried green tea was developed based on a nomogram model. The model was externally validated using actual measurement data and achieved an accuracy rate of 83.33%. Therefore, this study offers a scientific theoretical foundation for explaining the forecast and interference of the quality of ancient tree sun−dried green tea under abiotic stress.

Plain language summary Preterm birth (PTB) is the leading cause of neonatal morbidity and mortality, as well as other system immaturity problem. But as one of important environmental tobacco smoking sources of maternal secondhand smoking in the family, paternal smoking is worthy for deep exploring of its potential impacts on PTB. Moreover, evidence on the independent role of preconception paternal smoking is still lacking. Thus, we conducted a population-based retrospective cohort study to evaluate the association between paternal smoking and risk of PTB among among 5,298,043 reproductive-aged couples who participated in the National Free Pre-Pregnancy Checkups Project (NFPCP), regarding outcome events that occurred in 2010–2016. We found a significant association between paternal smoking and PTB. Smoking reduction should not only be advised to pregnant women but also to their partners to reduce PTB in their fetal. In addition, supporting patients to continue smoking reduction will be crucial when considering the adverse health outcome of smoking. Intervention of tobacco use before and during pregnancy, are critical for prevention of PTB. Avoiding both maternal and paternal smoking during pregnancy will benefit the developing fetus.

Plastids are a group of diverse organelles with conserved carotenoids synthesizing and sequestering functions in plants. They optimize the carotenoid composition and content in response to developmental transitions and environmental stimuli. In this review, we describe the turbulence and reforming of transcripts, proteins, and metabolic pathways for carotenoid metabolism and storage in various plastid types upon organogenesis and external influences, which have been studied using approaches including genomics, transcriptomics, proteomics, and metabonomics. Meanwhile, the coordination of plastid signaling and carotenoid metabolism including the effects of disturbed carotenoid biosynthesis on plastid morphology and function are also discussed. The “omics” insight extends our understanding of the interaction between plastids and carotenoids and provides significant implications for designing strategies for carotenoid-biofortified crops.

Abstract Crop yield is limited by water and nitrogen (N) availability. However, in Hexi Corridor of northwestern China, water scarcity and excessive fertilizer N in wheat (Triticum aestivum L.) production causes serious conflicts between water and N supply and crop demand. A field experiment was conducted from 2016 to 2018 to evaluate whether reducing of irrigation and fertilizer N will reduce grain yield of wheat. There were two irrigation quotas (192 and 240 mm) and three fertilizer N rates (135, 180, and 225 kg N ha−1). The results showed that reducing irrigation to 192 mm and N rate to 180 kg N ha−1 reduced water uptake, water uptake efficiency, and N uptake of spring wheat as compared to local practice (i.e., 240 mm irrigation and 225 kg N ha−1 fertilizer). Whereas, it improved water and N utilization efficiency, and water and N productivity. Consequently, the irrigation and N rate reduced treatment achieved the same quantity of grain yield as local practice. The path analysis showed that interaction effect between irrigation and N fertilization may attributable to the improvement of grain yield with lower irrigation and N rate. The enhanced water and N utilization allows us to conclude that irrigation quota at 192 mm coupled with fertilizer N rate at 180 kg N ha−1 can be used as an efficient practice for wheat production in arid irrigation areas.

Exposure to greenness may lead to a wide range of beneficial health outcomes. However, the effects of greenness on preterm birth (PTB) are inconsistent, and limited studies have focused on the subcategories of PTB. A total of 3,751,672 singleton births from a national birth cohort in mainland China were included in this study. Greenness was estimated using the satellite-based Normalized Difference Vegetation Index (NDVI) and Enhanced Vegetation Index with 500-m and 1,000-m buffers around participants’ addresses. The subcategories of PTB (20–36 weeks) included extremely PTB (EPTB, 20–27 weeks), very PTB (VPTB, 28–31 week), and moderate-to-late PTB (MPTB, 32–36 weeks). Gestational age (GA) was included as another birth outcome. We used logistic regression models and multiple linear regression models to analyze these associations throughout the entire pregnancy. We found inverse associations between greenness and PTB and positive associations between greenness and GA. Specifically, an increase of 0.1 NDVI exposure within a 500-m buffer throughout the entire pregnancy was significantly associated with decreases in PTB (odds ratio [OR], 0.930; 95% confidence interval [CI], 0.927–0.932), EPTB (OR, 0.820; 95% CI, 0.801–0.839), VPTB (OR, 0.913; 95% CI, 0.908–0.919), MPTB (OR, 0.934; 95% CI, 0.931–0.936), and an increase in GA (β = 0.050; 95% CI, 0.049–0.051 weeks). These results suggest the potential protective effects of greenness on PTB and its subcategories: MPTB, VPTB, and EPTB in China.

Abstract Background The universal two-child policy in China which was announced in October 2015 might affect the caesarean birth rate. Few studies reported the caesarean birth rate affected by the policy especially after the universal two-child policy period. This study aimed to demonstrate the caesarean birth rate changes before and after the universal two-child policy and further elaborate the underlying influencing factors. Methods This nationwide, retrospective, population-based study was based on National Free Pre-Pregnancy Check-ups Project (NFPCP). Couples planning to conceive in next 6 months were encouraged to participate in NFPCP. Baseline information was collected using a standardized questionnaire with a face-to-face interview, and medical examinations were conducted. Two rounds of follow-up interviews were then conducted by trained nurses to update pregnancy status and outcomes. NFPCP participants who completed deliveries before December 31, 2018, were included in the current study. We used marginal effect of year to examine the trend of caesarean birth rate over time and interrupted time series (ITS) analysis to determine impacts of the universal two-child policy on the trend of caesarean birth rate. Results A total of 9,398,045 participants were included in the final analysis. High-risk factors to increase caesarean birth rate were identified. In the current study, the standardized caesarean birth rate declined from 34.1% in 2013 to 31.8% in 2015 and increased to 35.6% in 2018. ITS analysis showed the caesarean birth rate decreased by 0.1% (95% CI 0.1–0.1) per month before the release of universal two-child policy, 1.3% (95% CI 0.6–2.1%) absolute drop during the policy release month, and increased by 0.2% (95% CI 0.1–0.2%) per month after the policy implementation. For the period after the policy release, the increasing trends were observed in rural participants and urban primiparas. The prevalence of caesarean birth rates within China varied regionally. Conclusions The decreasing trend of caesarean birth rate was reported after immediate release of the universal two-child policy. An increasing trend of caesarean birth rate was observed 2–3 years after the policy. It reminds us that the caesarean birth rate control is a long-lasting process and all the strategies need to be continually reinforced.

Transmission lines are most prone to faults in the transmission system, so high-precision fault diagnosis is very important for quick troubleshooting. There are some problems in current intelligent fault diagnosis research methods, such as difficulty in extracting fault features accurately, low fault recognition accuracy and poor fault tolerance. In order to solve these problems, this paper proposes an intelligent fault diagnosis method for high voltage direct current transmission lines (HVDC) based on Gramian angular difference field (GADF) domain and improved convolutional neural network (VGG16). This method first performs variational modal decomposition (VMD) on the original fault voltage signal, and then uses the correlation coefficient method to select the appropriate intrinsic mode function (IMF) component, and converts it into a two-dimensional image using the Gramian Angular Difference Field(GADF). Finally, the improved VGG16 network is used to extract and classify fault features adaptively to realize fault diagnosis. In order to improve the performance of the VGG16 fault diagnosis model, batch normalization, dense connection and global average pooling techniques are introduced. The comparative experimental results show that the model proposed in this paper can further identify fault features and has a high fault diagnosis accuracy. In addition, the method is not affected by fault type, transitional resistance and fault distance, has good anti-interference ability, strong fault tolerance, and has great potential in practical applications.

Chitosan oligosaccharide (COS), a degradation product of chitosan, is easily accessible, highly bioactive, non-toxic, and well-soluble in water. The effects of COS on the qualitative attributes of tomato fruits were investigated in the current study. COS was administered to tomato plants (Solanum lycopersicum cv. Ruixinghongniu) by foliar spray and root irrigation in alternate cycles at concentrations of 0.5 g·L−1 and 0.16 g·L−1, respectively. The experimental outcomes revealed that COS treatment promoted the coloring and softening of tomato fruits. Lycopene, vitamin C, fructose, and glucose levels increased by 49.0%, 25.4%, 30.2%, and 33.4%, respectively, in COS-treated ripe fruits compared to controls. The volatile metabolome showed that COS application also increased the release of ten volatiles correlated with consumer preference (1-penten-3-one, (E)-2-pentenal, (E)-3-hexen-1-ol, (E)-2-heptenal, 2-isobutylthiazole, phenylacetaldehyde, 2-phenylethanol, 6-methyl-5-hepten-2-one, 6-methyl-5-hepten-2-ol, and β-ionone), contributing to an improved tomato flavor. Moreover, increased transcript levels of genes participating in ethylene biosynthesis, perception, and response along with enhanced ethylene production were observed in COS-treated fruits, suggesting that COS may regulate tomato fruit quality via the ethylene pathway. Taken together, our results indicated that the pre-harvest application of COS could improve tomato fruit quality attributes.

Tea gray blight disease and its existing control measures have had a negative impact on the sustainable development of tea gardens. However, our knowledge of safe and effective biological control measures is limited. It is critical to explore beneficial microbial communities in the tea rhizosphere for the control of tea gray blight. In this study, we prepared conditioned soil by inoculating Pseudopestalotiopsis camelliae-sinensis on tea seedling leaves. Thereafter, we examined the growth performance and disease resistance of fresh tea seedlings grown in conditioned and control soils. Next, the rhizosphere microbial community and root exudates of tea seedlings infected by the pathogen were analyzed. In addition, we also evaluated the effects of the rhizosphere microbial community and root exudates induced by pathogens on the performance of tea seedlings. The results showed that tea seedlings grown in conditioned soil had lower disease index values and higher growth vigor. Soil microbiome analysis revealed that the fungal and bacterial communities of the rhizosphere were altered upon infection with Ps. camelliae-sinensis. Genus-level analysis showed that the abundance of the fungi Trichoderma, Penicillium, and Gliocladiopsis and the bacteria Pseudomonas, Streptomyces, Bacillus, and Burkholderia were significantly (p < 0.05) increased in the conditioned soil. Through isolation, culture, and inoculation tests, we found that most isolates from the induced microbial genera could inhibit the infection of tea gray blight pathogen and promote tea seedling growth. The results of root exudate analysis showed that infected tea seedlings exhibited significantly higher exudate levels of phenolic acids and flavonoids and lower exudate levels of amino acids and organic acids. Exogenously applied phenolic acids and flavonoids suppressed gray blight disease by regulating the rhizosphere microbial community. In summary, our findings suggest that tea plants with gray blight can recruit beneficial rhizosphere microorganisms by altering their root exudates, thereby improving the disease resistance of tea plants growing in the same soil.

Summary: Fruit softening indicated by firmness determines the texture, transportability, and shelf life of tomato products. However, the regulatory mechanism underlying firmness formation in tomato fruit is poorly understood. Here, we report the regulatory role of SlBES1, an essential component of brassinosteroid hormone signaling, in tomato fruit softening. We found that SlBES1 promotes fruit softening during tomato fruit ripening and postharvest storage. RNA-seq analysis suggested that PMEU1, which encodes a pectin methylesterase, might participate in SlBES1-mediated softening. Biochemical and immunofluorescence assays indicated that SlBES1 inhibited PMEU1-related pectin de-methylesterification. Further molecular and genetic evidence verified that SlBES1 directly binds to the E-box of PMEU1 to repress its expression, leading to fruits softening. Loss-of-function SlBES1 mutant generated by CRISPR-Cas9 showed firmer fruits and longer shelf life during postharvest storage without other quality alteration. Collectively, our results indicated the potential of manipulating SlBES1 to regulate firmness without negative consequence on visual and nutrition quality.

Background: Cytomegalovirus seroconversion during pregnancy is common and has a substantial risk of congenital infection with longterm sequale. Screening during pregnancy or vaccination have not been shown to be effective for eliminating congenital infections. Preconception screening policy has not been evaluated adequately in a large scale. This nationwide study aimed to investigate epidemiological features of cytomegalovirus seropositivity and its geographic variation among Chinese women planning a pregnancy to gather epidemiological evidence as an essential for developing novel prevention strategies.Method: This cross-sectional sero-epidemiological survey enrolled women intending to become pregnant within 6 months in mainland China during 2010–2012. The primary outcomes in this study were cytomegalovirus Immunoglobulin G and M seropositivity. Secondary outcomes were the associations between Immunoglobulin G and Immunoglobulin M, with socio-demographic characteristics, including age, occupation, education level, place of residence, and ethnicity. The overall seropositivity and regional disparity was analyzed on the individual and regional level, respectively.Results: This study included data from 1,564,649 women from 31 provinces in mainland China. Among participants, 38.6% (n = 603,511) were cytomegalovirus immunoglobulin G+, 0.4% (n = 6,747) were immunoglobulin M+, and 0.2% (n = 2,879) were immunoglobulin M+ and immunoglobulin G+. On individual level, participant's age, ethnicity, and residing region were significantly associated with IgG+, IgM+, and IgM+IgG+ (P < 0.001), while occupation, education level, and place of residence were not statistically significant (P > 0.05). On regional level, cytomegalovirus immunoglobulin G and immunoglobulin M seropositivity was highest in the eastern region (49.5 and 0.5%, respectively), and lowest in the western region (26.9 and 0.4%, respectively). This geographic variation was also noted at the provincial level, characterized by higher provincial immunoglobulin M+ and immunoglobulin G+ rates associated with higher immunoglobulin G seropositivity. In the subgroup analysis of immunoglobulin G seropositivity, areas of higher immunoglobulin G positivity had a higher rate of immunoglobulin M+, indicating an expected increased risk of reinfection and primary infection.Conclusions: A substantial proportion of women (>60%) were susceptible to cytomegalovirus in preconception period in China, and immunoglobulin G seropositivity was seen at a low-medium level with substantial geographic variation. Integration of cytomegalovirus antibody testing in preconception screening program based on regional immunoglobulin G seropositivity, should be considered to promote strategies directed toward preventing sero-conversion during pregnancy to reduce the risk of this congenital infection.

To avoid the impact of the transient saturation of a current transformer, a fast busbar protection method based on the initial traveling wave integrated active power differential principle is proposed in this paper. The Peterson equivalent model is applied to analyze the initial traveling wave distribution characteristics of the internal and external faults of the busbar. The initial traveling wave active powers of each sampling point on each transmission line connected to the busbar are calculated with the implementation of an S-transform. By introducing the integrated active power actuating quantity and active power braking quantity, a protection criterion with a characteristic braking ratio is proposed. The theoretical analysis and experimental simulation results show that the protection performance is sensitive and reliable, with a quick response and simple criterion, and is essentially not susceptible to the impacts of the initial fault angle, fault type, and fault resistance.

Background: To evaluate the associations of pre-conception body mass index (BMI), fasting plasma glucose (FPG) alone and their combination with pre-term birth (PTB) risk.Methods: We conducted a population-based retrospective cohort study with 4,987,129 reproductive-aged women, who participated in National Free Pre-Pregnancy Checkups Project in 2013–2016 and had a singleton delivery before December 2017 in China. All data analyses were conducted in 2018–2021.Results: A total of 339,662 (6.81%) women had pre-term deliveries. Compared with women with normal weight and normal glucose, underweight and normal weight were associated with PTB among hypoglycemia women, the adjusted odd ratios (aORs) were 1.24 (95% CI: 1.05–1.48) and 1.16 (95% CI: 1.07–1.25), respectively; underweight, overweight and obesity were associated with PTB among women with normal glucose, the aORs were 1.09 (95% CI: 1.08–1.10), 1.06 (95% CI: 1.05–1.07) and 1.08 (95% CI: 1.05–1.12), respectively; all the BMI groups were significantly associated with PTB among women with pre-diabetes or diabetes (P < 0.05). The dose-response relationships of BMI with PTB varied in different FPG level, with U-shaped curve in normal glucose and pre-diabetes women, J-shaped in diabetes women, L-shaped in hypoglycemia women. For FPG with PTB, the dose-response relationships were U-shaped in normal weight, overweight, and obesity women, and L-shaped in underweight women.Conclusion: We found that the associations of PTB with BMI varied with levels of FPG, and associations of PTB with FPG varied with levels of BMI. There was a synergistic effect on PTB risk due to abnormal weight and glycemia besides a conventional main effect derived from either of them. Achieving desirable weight and glucose control before conception should be advised.

Carotenoids are organic pigments that play an important role in both plant coloration and human health; they are a critical subject in molecular breeding due to growing demand for natural molecules in both food and medicine. In this study, we focus upon characterizing BoaCRTISO, the carotenoid isomerase gene before the branch of the carotenoid biosynthetic pathway, which is expressed in all organs and developmental stages of Chinese kale, and BoaCRTISO, which is located in the chloroplast. The expression of BoaCRTISO is induced by strong light, red and blue combined light, and gibberellic acid treatment, but it is suppressed by darkness and abscisic acid treatment. We obtained BoaCRTISO-silenced plants via virus-induced gene silencing technology, and the silence efficiencies ranged from 52 to 77%. The expressions of most carotenoid and chlorophyll biosynthetic genes in BoaCRTISO-silenced plants were downregulated, and the contents of carotenoids and chlorophyll were reduced. Meanwhile, BoaCRTISO-silenced plants exhibited phenotypes of yellowing leaves and inhibited growth. This functional characterization of BoaCRTISO provides insight for the biosynthesis and regulation of carotenoid in Chinese kale.

An unknown root-knot nematode was found at high density on grape roots collected from Yunnan Province. Morphometric traits and measurements, isozyme phenotypes, and molecular analysis clearly differentiated this nematode from previously described root-knot nematodes. This new species is described, illustrated and named Meloidogyne vitis sp. nov. The new species can be distinguished from other Meloidogyne spp. by a unique combination of characters. Females display a prominent neck, an excretory pore is located on the ventral region between 23rd and 25th annule behind lips, an EP/ST ratio of approximately 2.5 (1.98-2.96), a perineal pattern with two large and prominent phasmids, and a labial disc fused with the medial lips to form a dumbbell-shaped structure. Males display an obvious head region, a labial disc fused with the medial lips to form a dumbbell-shaped structure, no lateral lips, a prominent slit-like opening between the labial disc and medial lips, a distinct sunken appearance of the middle of the medial lips, and four incisures in the lateral field. Second-stage juveniles are characterized by a head region with slightly wrinkled mark, a labial disc fused with the medial lips to form a dumbbell-shaped structure, a slightly sunken appearance of the middle of the medial lips, a slit-like amphidial openings between the labial disc and lateral lips, and four incisures in the lateral field. The new species has rare Mdh (N3d) and Est phenotypes (VF1). Phylogenetic analysis based on ITS1-5.8S-ITS2, D2D3 fragments of rDNA, and coxI and coxII fragments of mtDNA sequences clearly separated the new species from other root-knot nematodes, and the closest relative was Meloidogyne mali. Meloidogyne mali was collected for amplifying these sequences as mentioned above, which were compared with the corresponding sequences of new species, the result showed that all of these sequences with highly base divergence (48-210 base divergence). Moreover, sequence characterized amplified region (SCAR) primers for rapid identification of this new species were designed.

The aroma profile of sun-dried black tea (SBT) was identified by headspace solid–phase microextraction (HS–SPME) coupled with gas chromatography–mass spectrometry (GC–MS) and gas chromatography–olfactometry (GC–O). A total of 37 scents were captured by using the GC–O technique, and 35 scents with odor intensities ranging from 1.09 ± 1.93 to 9.91 ± 0.29 were identified. Twenty-one compounds were further identified as key odor-active compounds with odor activity values (OAVs) greater than or equal to one. These key odor-active compounds were restructured with their detected concentrations, and the aroma profile of the selected SBT sample was successfully imitated to a certain extent. An omission test was performed by designing 25 models and confirmed that (E)-β-damascenone, β-ionone, dihydro-β-ionone, linalool, and geraniol were the key odor-active compounds for the aroma profile of SBT. Meanwhile, phenylethyl alcohol, (E)-2-decenal, hexanal, and methyl salicylate were also important to the aroma profile of SBT. This study can provide theoretical support for the improvement of the aroma quality of sun-dried black tea.

In order to remedy the current problem of having been buffeted by competing requirements for both protection sensitivity and quick reaction of High Voltage Direct Current (HVDC) transmission lines simultaneously, a new intelligent fault identification method based on Random Forests (RF) for HVDC transmission lines is proposed. S transform is implemented to extract fault current traveling wave of 8 frequencies and calculate the fluctuation index and energy sum ratio, in which the wave index is used to identify internal and external faults, and energy sum ratio is used to identify the positive and negative pole faults occurred on the transmission line. The intelligent fault identification model of RF is established, and the fault characteristic sample set of HVDC transmission lines is constructed by using multi-scale S transform fluctuation index and multi-scale S-transform energy sum ratio. Training and testing have been carried out to identify HVDC transmission line faults. According to theoretical researches and a large number of results of simulation experiments, the proposed intelligent fault identification method based on RF for HVDC transmission lines can effectively solve the problem of protection failure caused by inaccurate identification of traditional traveling wave wavefront or wavefront data loss. It can accurately and quickly realize the identification of internal and external faults and the selection of fault poles under different fault distances and transitional resistances, and has a strong ability to withstand transitional resistance and a strong ability to resist interference.

Tomato is susceptible to chilling injury during cold storage. In this study, we found that low temperature promoted the expression of brassinosteroid (BR) biosynthetic genes in tomato fruits. The overexpression of SlCYP90B3 (SlCYP90B3-OE), a key BR biosynthetic gene, alleviated the chilling injury with decreased electrical conductivity and malondialdehyde. In SlCYP90B3-OE tomato fruits, the activities of antioxidant enzymes, including ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), were markedly increased, while the activity of membranous lipolytic enzymes, lipoxygenase (LOX), and phospholipase D (PLD), were significantly decreased when compared with the wild-type in response to cold storage. Furthermore, the expression level of the cold-response-system component, SlCBF1, was higher in SlCYP90B3-OE fruits than in the wild-type fruits. These results indicated that SlCYP90B3 might be involved in the chilling tolerance of tomato fruits during cold storage, possibly by regulating the antioxidant enzyme system and SlCBF1 expression.

Background: Most evidences regarding ambient PM2.5 or PM10 (particulate matter of median aerodynamic diameter ≤2.5 μm or ≤10 μm) and preterm birth (PTB) come from western countries which has relatively low PM pollution exposure, and the results are still inconsistent. This study aims to examine whether exposure to high concentrations of PM2.5 or PM10 was associated with PTB (

ζ-Carotene desaturase (ZDS) is an important enzyme in carotenoid biosynthesis. Here, the Brassica oleracea var. alboglabra ZDS (BoaZDS) gene was cloned from Chinese kale via reverse transcription-polymerase chain reaction (RT-PCR) and deposited in GenBank (accession number KY662297). The BoaZDS gene contains an open reading frame of 1 686 bp that encodes a 561-amino acid protein. Sequence analysis indicates that the ZDS protein is apparently conserved during plant evolution and is most closely related to B. oleracea var. capitata and B. rapa. The promoter sequence of the BoaZDS gene was predicted to harbor several cis-acting elements that are related to light and phytohormone responses. Semi-quantitative RT-PCR analysis showed that BoaZDS expression varied among different developmental stages and organs. Relative ZDS expression remained stable during germination and seedling stages and rapidly increased at the mature leaf stage. The leaves showed the highest ZDS expression levels compared to the other organs. ZDS expression decreased in all flower tissues during blooming. The fused protein of BoaZDS was obtained by prokaryotic expression. Heterologous expression of BoaZDS in Escherichia coli confirmed that BoaZDS encodes a functional ζ-carotene desaturase that increases β-carotene accumulation in E. coli cells harboring a β-carotene-producing plasmid. The findings of the present study provide a molecular basis for the elucidation of ZDS gene function in Chinese kale. Keywords: Chinese kale, Brassica oleracea var. alboglabra Bailey, carotenoids, ζ-carotene desaturase (ZDS), cloning, gene expression, prokaryotic expression, heterologous expression

Abstract Background To assess socioeconomic inequality in periconceptional folic acid supplementation in China. Methods We used data of periconceptional folic acid (FA) supplementation of rural Chinese women from the National Free Preconception Health Examination Project from 2010 to 2012 and socioeconomic level data from the National Bureau of Statistics. We used logistic models to assess the associations between the prevalence of taking FA and the sociodemographic characteristics of the participants, the couples, and the socioeconomic levels of their region of residence. Results Of the 907,720 included women, 682,315 (75.62%) of the women reported taking FA. The prevalence of FA supplementation was significantly higher in participants aged 21–29 (75.87%) than in those women aged 40–49 (68.44%, p

Objectives: Infection with rubella virus during pregnancy can result in congenital defects and adverse pregnancy outcomes. The risk of rubella infection is greatly determined by the level of rubella antibodies in the serum. A survey of rubella antibody seronegativity rates was conducted in 780 000 women in rural China who were planning a pregnancy, in order to evaluate the herd susceptibility in different age groups and by high, middle, and low GDP per capita regions. Methods: In order to evaluate the herd susceptibility to rubella, a nationwide population-based study of rural Chinese women who were planning to have a baby and who were aged 21–49 years was instigated. As a part of the National Free Pre-conception Health Examination Project covering 29 provinces in 2012, a physical check-up program was provided to women who planned to become pregnant within the next 6 months. All medical data were from serological samples tested by ELISA, and the participants’ immunity status was categorized based on levels of rubella antibodies. Economic data were also collected to explore the association between herd susceptibility and socioeconomic characteristics in the women of childbearing age. Results: A total 264 306 of 782 293 recruited women preparing for pregnancy tested susceptible to rubella (33.79%). The seronegativity rate in women with a history of vaccination was significantly lower than that in women who had not received the vaccination or did not know their vaccination history (23.76%, 33.70%, and 35.68%, respectively). The seronegativity rates were 26.89%, 37.86%, and 32.61% in high, middle, and low GDP per capita areas, respectively. After stratified analysis and adjusting for other factors by multiple logistic regression, the lower seronegativity rates in women in high GDP per capita regions compared to women in middle and low GDP per capita regions remained in the different age groups and subgroups of immunization history. Conclusions: There is a clear difference in rubella-specific susceptibility among rural women preparing for pregnancy of different sociodemographic and economic backgrounds. The number of rubella-susceptible rural women preparing for pregnancy, especially in relatively low GDP per capita regions in China, was high. Offering rubella vaccination to women who are rubella-susceptible and who plan to become pregnant should become one of the priorities in the field of public health work in China.

Abstract Obesity has been increasing worldwide. Data on obesity status among men of reproductive age are scarce. This study aimed to assess the national prevalence and trend of overweight and obesity among reproductive-age men in rural China. In the nationwide population-based study, data were obtained from the National Free Preconception Health Examination Project in rural China from 2010 to 2014. Weight and height were measured in 16 161 982 men aged 15–49 years and their female partners (15 997 739 participants aged 15–49 years) before conception, and body mass index (BMI) was calculated. We found that the prevalence of combined overweight and obesity among men was 33.8% (33.7–33.9%) according to Chinese criteria (BMI ≥ 24.0 kg/m2), the corresponding rates of obesity were 6.3% (6.2–6.4%; BMI ≥ 28.0 kg/m2), respectively. The prevalence varied in different ethnic groups, geographic regions, age, education and GDP levels, and increasing trend was observed over the 5-year study period. In addition, 45.8% of couples had at least one of them as overweight or obesity. About one third of men aged 15–49 years before conception in rural China are overweight or obese. Overweight/obesity clusters in families, which highlights the importance of family involvement of early prevention of obesity in China.

Abstract We aimed to assess the prevalence of chronic kidney diseases (CKD) markers among the married residents with fertility desire in rural China. Demographic and clinical data were collected from the National Free Pre-Conception Health Examination Project. Estimated glomerular filtration rate (eGFR)

BACKGROUND:Diabetes mellitus (DM) increases the risk of adverse maternal and neonatal outcomes, and optimization of glycemic control during pregnancy can help mitigate risks associated with diabetes. However, studies seldom focus precisely on maternal blood glucose level prior to pregnancy. We aimed to evaluate the associations between preconception blood fasting plasma glucose (FPG) level and subsequent pregnancy outcomes. METHODS AND FINDINGS:We conducted a population-based retrospective cohort study among 6,447,339 women aged 20-49 years old who participated in National Free Pre-Pregnancy Checkups Project and completed pregnancy outcomes follow-up between 2010 and 2016 in China. During the preconception health examination, serum FPG concentration was measured, and self-reported history of DM was collected. Women were classified into three groups (normal FPG group: FPG < 5.6 mmol/L and no self-reported history of DM; impaired fasting glucose [IFG]: FPG 5.6-6.9 mmol/L and no self-reported history of DM; and DM: FPG ≥ 7.0 mmol/L or self-reported history of DM). The primary outcomes were adverse pregnancy outcomes, including spontaneous abortion, preterm birth (PTB), macrosomia, small for gestational age infant (SGA), birth defect, and perinatal infant death. Logistic regression model was used to calculate odds ratio (OR) and 95% confidence interval (CI) after adjusting for confounding variables. The mean age of women was 25.24 years, 91.47% were of Han nationality, and 92.85% were from rural areas. The incidence of DM and IFG was 1.18% (76,297) and 13.15% (847,737), respectively. Only 917 (1.20%) women reported a history of DM (awareness of their DM status), of whom 37.28% (337) had an elevated preconception FPG level (≥ 5.6 mmol/L), regarded as noncontrolled DM. A total of 1,005,568 (15.60%) women had adverse pregnancy outcomes. Compared with women with normal FPG, women with IFG had higher risks of spontaneous abortion (OR 1.08; 95% CI 1.06-1.09; P < 0.001), PTB (1.02; 1.01-1.03; P < 0.001), macrosomia (1.07; 1.06-1.08; P < 0.001), SGA (1.06; 1.02-1.10; P = 0.007), and perinatal infant death (1.08; 1.03-1.12; P < 0.001); the corresponding ORs for women with DM were 1.11 (95% CI 1.07-1.15; P < 0.001), 1.17 (1.14-1.20; P < 0.001), 1.13 (1.09-1.16; P < 0.001), 1.17 (1.04-1.32; P = 0.008), and 1.59 (1.44-1.76; P < 0.001). Women with DM also had a higher risk of birth defect (OR 1.42; 95% CI 1.15-1.91; P = 0.002). Among women without self-reported history of DM, there was a positive linear association between FPG levels and spontaneous abortion, PTB, macrosomia, SGA, and perinatal infant death (P for trend

Due to the characteristics of T-connection transmission lines, a new method for T-connection transmission lines fault identification based on current reverse travelling wave multi-scale S-transformation energy entropy and limit learning machine is proposed. S-transform are implemented on the faulty reverse traveling waves measured by each traveling wave protection unit of the T-connection transmission line, the reverse travelling wave energy entropies under eight different frequencies are respectively calculated, and a T-connection transmission line fault characteristic vector sample set are thus formed. Establish an intelligent fault identification model of extreme learning machines, and use the sample set for training and testing to identify the specific faulty branch of the T-connection transmission line. The simulation results show that the proposed algorithm can accurately and quickly identify the branch where the fault is located on the T-connection transmission line under various operation conditions.

To overcome the contradiction between speed and reliability in existing busbar protection schemes, a new busbar protection algorithm based on a polarity comparison of fundamental frequency currents is proposed. The algorithm extracts the fundamental frequency components of the fault reference current and the virtual current through a wavelet transform. The angle between the two currents is used to characterize the polarity relationship. The polarities of the virtual current and the reference current are the same when an internal fault occurs, and the angle will be small. The polarities of the two currents are opposite for an external fault, in which case the angle is larger. By analysing the variation characteristics of the angle between faults inside and outside busbar, a protection criterion is established, and the fault area is determined. In simulation results based on PSCAD/EMTDC, the algorithm can quickly and reliably identify the faults inside and outside the busbar area, and its performance is not affected by the initial fault angle, fault resistance, fault type or capacitor voltage transformer (CVT) transmission characteristics.

R2R3-MYB transcription factor MYB51 is known to control indole glucosinolate (indole GSL) biosynthesis in Arabidopsis. Here, two copies of BoaMYB51 have been isolated in Chinese kale (Brassica oleracea var. alboglabra Bailey), designated BoaMYB51.1 and BoaMYB51.2, which exhibit overlapping but distinct expression levels among different organs and respond to signaling molecules in a similar pattern. It has been demonstrated a structural and functional conservation between BoaMYB51s and AtMYB51 by phylogenetic analysis, complementation studies and transient expression assay. To further investigate the transcriptional mechanism, we identified the transcriptional activation domain (TAD) and putative interacting proteins of BoaMYB51s by means of yeast (Saccharomyces cerevisiae) two hybrid. Using tobacco (Nicotiana benthamiana) transient expression assay, we confirmed that the carboxy-end is required for transcriptional activation activity of BoaMYB51s. In addition, several BoaMYB51-interacting proteins have been identified by yeast two-hybrid screening. These results provide important insights into the molecular mechanisms by which MYB51 transcriptionally regulates indole GSL biosynthesis.

BackgroundEpidemiological evidence on the association between long‐term exposure to ambient fine particulate matter (with an aerodynamic diameter ≤2.5 μm; PM2.5) and hypertension is mixed. We investigated the long‐term association between ambient fine particles and hypertension in reproductive‐age adults. Methods and ResultsThis analysis included 39 348 119 reproductive‐age (20–49 years) participants from the National Free Preconception Health Examination Project from April 22, 2010 to December 31, 2015 across China. The estimation of annual average ambient PM2.5 concentrations for each community was realized through using satellite‐based spatial statistical models. Linear mixed models and 2‐level logistic regressions adjusted for potential confounders with natural cubic splines were used to investigate the shape of PM2.5–blood pressure and PM2.5‐hypertension, respectively. The effect modification by sex, obesity, smoking status, age, diabetes mellitus, urbanity, race, and region was also taken into account. The concentration‐response relationship between PM2.5 and hypertension was nonlinear, with a threshold concentration of 47.9 μg/m3. The odds ratio of hypertension related to a 10‐μg/m3 increase in PM2.5 above threshold was 1.010 (95% confidence interval, 1.007–1.012). A 10‐μg/m3 increase in PM2.5 above threshold corresponded to a 0.569 (95% confidence interval, 0.564–0.573) mm Hg elevation in systolic blood pressure and a 0.384 (95% confidence interval, 0.381–0.388) mm Hg elevation in diastolic blood pressure. There were 2.3% (95% confidence interval, 2.2%–2.4%) of the hypertension cases that could be attributed to PM2.5 exposures in reproductive‐age adult populations. ConclusionsLong‐term exposures to PM2.5 above certain levels might increase population risk for hypertension and might be responsible for China's avoidable hypertension burden in reproductive‐age adults.

Background: Preterm birth is the leading cause of child death in children younger than 5 years. Large cohort studies in developed countries have shown that maternal hepatitis B virus infection is associated with preterm birth, but there is little reliable evidence from China and other developing countries, where hepatitis B virus prevalence is intermediate or high. Hence, we designed this study to investigate the association between pre-pregnancy hepatitis B virus infection and risk of preterm and early preterm birth. Methods: Between Jan 1, 2010, and Dec 31, 2012, we did a population-based cohort study using data from 489 965 rural women aged 21–49 years who had singleton livebirths from 220 counties of China who participated in the National Free Preconception Health Examination Project. Participants were divided into three groups according to their pre-pregnancy status of hepatitis B virus infection: women uninfected with hepatitis B virus (control group), women who were HBsAg positive and HBeAg negative (exposure group 1), and women who were both HBsAg and HBeAg positive (exposure group 2). The primary outcome was preterm birth (gestation at less than 37 weeks). We used log-binomial regression to estimate adjusted risk ratios (aRR) of preterm birth for women with pre-pregnancy hepatitis B virus infection, and risk of early preterm birth (gestation less than 34 weeks). Findings: 489 965 women met inclusion criteria and were included in this study; of these, 20 827 (4·3%) were infected with hepatitis B virus. Compared with women who were not infected with hepatitis B virus, women who were HBsAg positive and HBeAg negative had a 26% higher risk of preterm birth (aRR 1·26, 95% CI 1·18–1·34) and women who were both HBsAg and HBeAg positive had a 20% higher risk of preterm birth (aRR 1·20, 1·08–1·32). Compared with women who were not infected with hepatitis B virus, women who were HBsAg positive and HBeAg negative manifested an 18% higher risk of early preterm birth (gestation less than 34 weeks; aRR 1·18, 1·04–1·34) and women who were both HBsAg and HBeAg positive had a 34% higher risk of early preterm birth (aRR 1·34, 1·10–1·61). Maternal pre-pregnancy hepatitis B virus infection was independently associated with higher risk of preterm birth and early preterm birth. These associations were similar in subgroups of participants as defined by baseline characteristics. Interpretation: Besides mother-to-child transmission, the risk of preterm birth in women infected with hepatitis B virus should not be neglected. Comprehensive programmes that focus on early detection of hepatitis B virus infection before pregnancy and provide appropriate medical intervention for women infected with hepatitis B virus before and during pregnancy would be helpful in improving maternal and neonatal outcomes and reducing child mortality. Funding: Chinese Association of Maternal and Child Health Studies.

Background The effect of second‐hand smoking, especially husband smoking, on wife's hypertension has not been well studied. The current study was aimed to assess the association of husband smoking with wife's hypertension among females aged 20 to 49 years. Methods and Results This study included 5 027 731 females along with their husbands from the National Free Pre‐pregnancy Checkup Projects conducted across 31 provinces in China in 2014. Smoking/passive smoking status was collected by a standard questionnaire and blood pressure was measured by an electronic device after 10 minutes rest. Odds ratios and their corresponding 95% CIs for female hypertension were estimated according to smoking status of husband and wife, husbands' smoking amount, and cumulative exposure to husband smoking. Compared with neither‐smoker group, the multivariable‐adjusted odds ratio for female hypertension was 1.28 (1.27–1.30), 1.53 (1.30–1.79), and 1.50 (1.36–1.67) in husband‐only, wife‐only, and mixed group, respectively. Furthermore, a higher risk of having hypertension was associated with amount and cumulative exposure of husband smoking. For example, compared with neither‐smoker, the multivariate‐adjusted odds ratio was 1.22 (1.19–1.25), 1.24 (1.21–1.26), 1.32 (1.26–1.37), 1.37 (1.34–1.41), and 1.75 (1.64–1.87) for females whose husband smoked 1 to 5, 6 to 10, 11 to 15, 16 to 20, and ≥21 cigarettes per day, respectively (Pfor trend

The effects of long-term freezer storage and different defrosting methods on the retention of glucosinolates, vitamin C, and total phenols in frozen broccoli florets were investigated in the present study. Frozen broccoli florets were stored in a freezer at −20 °C for 165 days or subjected to defrosting by three different house-hold thawing methods (water, air, and refrigerator defrosting). Results showed that all glucosinolates were well preserved, while vitamin C and total phenols were reduced by less than 12% and 19% of the control, respectively, during the storage. Besides, refrigerator and air defrosting were better than water defrosting in glucosinolates retention, and refrigerator defrosting was the best in vitamin C preservation. No difference was observed in reserving phenolic compounds among the three methods. In conclusion, long-term freezer storage is an excellent way to preserve broccoli florets, and refrigerator defrosting is the best way to maintain the nutritional compounds in frozen broccoli florets.