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7 results on '"Songquan Yang"'

1. A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification

Author

MingShu Wang, AnChun Cheng, Rui Yang, JinLong Yang, LiZhi Fu, SongQuan Yang, SuHui Zhang, Liu Yang, and ZhiYong Xu

Subjects
Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background Goose parvovirus (GPV) is a Dependovirus associated with latent infection and mortality in geese. Currently, in a worldwide scale, GPV severely affects geese production. The objective of this study is to develop a loop-mediated isothermal amplification (LAMP) method for the sensitive, rapid, and inexpensive detection of GPV in the field. Results A set of six specific primers was designed by targeting the GPV VP3 DNA. With Bst DNA polymerase large fragment, the target DNA could be amplified at 65°C as early as 20 min of incubation in a simple water bath. A positive reaction was identified through the detection of the LAMP product by color change visible to the naked eye. The detection limit of the assay was 28 copies/μl of plasmid pVP3, and with equal sensitivity and specificity to fluorescent quantitative real-time PCR (FQ-PCR). Conclusions The high sensitivity, specificity, and simplicity, as well as the high throughput, make this method suitable for specific detection of GPV infection in both field conditions and laboratory settings. The utilization of complicated equipment and conduct of technical training on the GPV LAMP were not necessary.

Published
2010
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2. A Highly Efficient Annealing Process With Supercritical N2O at 120 °C for SiO2/4H–SiC Interface

Author

Yang Mingchao, Yue Hao, Songquan Yang, Menghua Wang, Chuanyu Han, Li Geng, Jinwei Qi, and Weihua Liu

Subjects
010302 applied physics, Materials science, Annealing (metallurgy), Oxide, 01 natural sciences, Supercritical fluid, Electronic, Optical and Magnetic Materials, Carbide, Hysteresis, chemistry.chemical_compound, chemistry, Chemical engineering, Electric field, 0103 physical sciences, Silicon carbide, Field-effect transistor, Electrical and Electronic Engineering
Abstract

A novel post-oxidation annealing (POA) process with supercritical N2O (SCN2O) fluid is reported to be highly effective in improving the interface properties of the SiO2/4H–silicon carbide (SiC) (0001) systems. After SCN2O POA, the interface state density reduces to $2.8 \times 10^{{11}}$ eV−1cm−2, which is about 3.5 times lower than that after a traditional high-temperature N2O POA process. Meanwhile, the highest oxide critical electric field shows an increase of 18.19% and the near-interfacial oxide traps is reduced by 69.90% compared with that after N2O POA process. The process temperature is as low as 120 °C. The significantly reduced processing temperature avoids additional defect generation while the supercritical state provides a stronger nitridation effect. SCN2O annealing is a promising candidate for POA process toward high-performance SiC power metal-oxide-semiconductor field effect transistors (MOSFETs).

Published
2021

4. Interface optimization of 4H-SiC (0001) MOS structures with supercritical CO2 fluid

Author

Songquan Yang, Chuanyu Han, Yang Mingchao, Yue Hao, Weihua Liu, Menghua Wang, Liu Jiang, and Li Geng

Subjects
010302 applied physics, Materials science, Passivation, business.industry, Interface (computing), General Engineering, Oxide, General Physics and Astronomy, 02 engineering and technology, Semiconductor device, 021001 nanoscience & nanotechnology, 01 natural sciences, Supercritical fluid, chemistry.chemical_compound, chemistry, Electric field, Reaction model, State density, 0103 physical sciences, Optoelectronics, 0210 nano-technology, business
Abstract

Supercritical CO2 fluid is reported as an effective media in optimizing the SiO2/SiC interface in 4H-SiC(0001) MOS structures at a temperature as low as 150 °C. After SCCO2 treatment, the breakdown electric field is improved to 10.7 MV cm−1. The near-interfacial oxide traps is decreased from 1.62 × 1011 to 1.84 × 1010 cm−2. The interface state density at 0.2 eV below E C is reduced from 6 × 1012 to 2.5 × 1012 eV−1 cm−2. A dynamic reaction model is proposed to explain the defect passivation reaction. This technique can be effectively applied to interface semiconductor devices to enhance performance.

Published
2020

5. ISOLATION AND IDENTIFICATION OFMICROSPORUM CANISFROM ASIAN ELEPHANTS (ELEPHAS MAXIMUS) IN THE CHONGQING ZOO, CHINA

Author

Denghu Wu, Mi Benzhong, Hu Juan, Yang Yang, Qiao Xingfang, Li Wei, Chen Jiankang, and SongQuan Yang

Subjects
0301 basic medicine, China, Veterinary medicine, Elephants, Fungus, law.invention, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, Elephas, law, DNA, Ribosomal Spacer, Animals, Dermatomycoses, Microsporum, Microsporum canis, DNA, Fungal, Pathogen, Polymerase chain reaction, General Veterinary, biology, General Medicine, biology.organism_classification, Isolation (microbiology), 030104 developmental biology, Animals, Zoo, Animal Science and Zoology
Abstract

Skin diseases affect millions of people and animals worldwide, including Asian elephants. This study sought to determine the pathogen of skin diseases that occurred in Asian elephants in Chongqing Zoo, China. The isolated fungus was identified through its cultural characteristics, morphology, and polymerase chain reaction (PCR) amplification. The PCR amplification using common fungal primers (ITS1 and ITS4) determined that the pathogen was 99.7% homologous to Microsporum canis. This is the first report on elephants infected with Microsporum canis in China.

Published
2016

7. Polydopamine-coated paper-stack nanofibrous membranes enhancing adipose stem cells' adhesion and osteogenic differentiation.

Author

Liangpeng Ge, Qingtao Li, Yong Huang, Songquan Yang, Jun Ouyang, Shoushan Bu, Wen Zhong, Zuohua Liu, and Xing, Malcolm M. Q.

Abstract

In the fabrication of 3-D complex tissues for implantation, layer-by-layer (LBL) electrospun nanofibrous scaffolds have recently received intensive interest. However, poor cell adhesion and cell expansion between the layers in an LBL stack remain important issues. In this study, we report a mussel-inspired, biomimetic approach to functionalize the surface of PCL/gelatin nanofibrous membranes coated with poly (dopamine) (PDA). Our study demonstrates that a PDA coating on electrospun PCL/gelatin nanofibers leads to a significant change in their surface properties and a higher adhesion force. Furthermore, we found that PDA coating promotes the adhesion and growth of adipose stem cells (ADSCs). In 3-D LBL stacked scaffolds, more cells survived in a PAD-coated scaffold than in a non-coated one. The PDA coating was further demonstrated to promote the osteogenic differentiation of ADSCs in LBL paper-stacking membranes. Our study suggests that PDA-coated paper-stacking nanofiber membranes present a facile and economic method for the development of 3D tissue engineering. [ABSTRACT FROM AUTHOR]

Published
2014
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